Cryba2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryba2
Entrez Gene ID	286925
Full Name	crystallin, beta A2
General protein information	Preferred Names crystallin, beta A2 Names beta-crystallin A2 betaA2-crystallin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33
Summary	human homolog is a structural constituent of the eye lens [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_173140.1	NP_775163.1	beta-crystallin A2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cryba2a	NP_001002049.1
Homo sapiens (human)	CRYBA2	NP_476435.1
	CRYBA2	XP_001096534.1
Mus musculus (house mouse)	Cryba2	NP_067516.1
Canis lupus familiaris (dog)	CRYBA2	XP_545651.3
Rattus norvegicus (Norway rat)	Cryba2	NP_775163.1
Danio rerio (zebrafish)	cryba2b	NP_001002584.1
Xenopus tropicalis (tropical clawed frog)	LOC100496418	XP_002933987.1
Pan troglodytes (chimpanzee)	CRYBA2	XP_001159761.1
Bos taurus (cattle)	CRYBA2	NP_776949.1
Gallus gallus (chicken)	CRYBA2	NP_989510.1

Gene Ontology
Bibliography

Related articles in PubMed

First mutation in the ?A2-crystallin encoding gene is associated with small lenses and age-related cataracts.
Puk O, Ahmad N, Wagner S, Hrab? de Angelis M, Graw J
Investigative ophthalmology & visual science52(5)2571-6(2011 Apr)

Energetics of domain-domain interactions and entropy driven association of beta-crystallins.
Sergeev YV, Hejtmancik JF, Wingfield PT
Biochemistry43(2)415-24(2004 Jan)

Identification of the human beta A2 crystallin gene (CRYBA2): localization of the gene on human chromosome 2 and of the homologous gene on mouse chromosome 1.
Hulsebos TJ, Cerosaletti KM, Fournier RE, Sinke RJ, Rocchi M, Marzella R, Jenkins NA, Gilbert DJ, Copeland NG
Genomics28(3)543-8(1995 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cryba2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryba2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03546	NM_173140.1 Latest version!	Rattus norvegicus crystallin, beta A2 (Cryba2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryba2 ( NM_173140.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775163.1 Rattus norvegicus crystallin, beta A2 (Cryba2), mRNA.

Price & Availability↑

CloneID	ORa03546
Clone ID Related Accession (Same CDS sequence)	NM_173140.1
Accession Version	NM_173140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	beta-crystallin A2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY158891.1. ##Evidence-Data-START## Transcript exon combination :: AY158891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGCG CCCCGGCACC GGGCCCGGCA CCCGCTTGCC TCACGCTCTG GGACGAGGAG 
GACTTCCAGG GTCGTCGCTG CCGGCTACTG AGCGACTGTG CCAACGTCTG TGAGCGGGGA 
GCCCTGCGGA GGGTGCGCTC AGTCAAGGTG GAAAACGGCG CATGGGTGGC CTTTGAGTAC 
CCCGACTTCC AGGGACAGCA GTTCATTCTA GAGAAGGGAG ACTATCCTTG CTGGAGTGCC 
TGGAGCGGTA GCAGCGGCCA CCACAGCAAC CAATTGCTGT CCTTCAGGCC AGTGCTCTGT 
GCGAACCACA GTGACAGCCG TGTGACCCTG TTTGAGGGGG AAAACTTCCA GGGCTGCAAG 
TTTGAACTCA GTGATGACTA CCCATCACTG CCTTCCATGG GCTGGACCAG CAAAGATGTG 
GGTTCCCTCA AAGTCAGCTC TGGAGCGTGG GTGGCCTACC AATACCCTGG CTACCGAGGC 
TACCAGTATG TCTTAGAACG GGACCGGCAC AGTGGGGAGT TCCGTACCTA CAATGACTTT 
GGCACACAGG CCCATACTGG ACAGCTGCAG TCCATTCGAA GAGTCCAGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775163.1
CDS	37..630
Translation	MSSAPAPGPAPACLTLWDEEDFQGRRCRLLSDCANVCERGALRRVRSVKVENGAWVAFEYPDFQGQQFILEKGDYPCWSAWSGSSGHHSNQLLSFRPVLCANHSDSRVTLFEGENFQGCKFELSDDYPSLPSMGWTSKDVGSLKVSSGAWVAYQYPGYRGYQYVLERDRHSGEFRTYNDFGTQAHTGQLQSIRRVQH

Target ORF information:

RefSeq Version	NM_173140.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, beta A2 (Cryba2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173140.1

ORF Insert Sequence:

ATGAGCAGCG CCCCGGCACC GGGCCCGGCA CCCGCTTGCC TCACGCTCTG GGACGAGGAG 
GACTTCCAGG GTCGTCGCTG CCGGCTACTG AGCGACTGTG CCAACGTCTG TGAGCGGGGA 
GCCCTGCGGA GGGTGCGCTC AGTCAAGGTG GAAAACGGCG CATGGGTGGC CTTTGAGTAC 
CCCGACTTCC AGGGACAGCA GTTCATTCTA GAGAAGGGAG ACTATCCTTG CTGGAGTGCC 
TGGAGCGGTA GCAGCGGCCA CCACAGCAAC CAATTGCTGT CCTTCAGGCC AGTGCTCTGT 
GCGAACCACA GTGACAGCCG TGTGACCCTG TTTGAGGGGG AAAACTTCCA GGGCTGCAAG 
TTTGAACTCA GTGATGACTA CCCATCACTG CCTTCCATGG GCTGGACCAG CAAAGATGTG 
GGTTCCCTCA AAGTCAGCTC TGGAGCGTGG GTGGCCTACC AATACCCTGG CTACCGAGGC 
TACCAGTATG TCTTAGAACG GGACCGGCAC AGTGGGGAGT TCCGTACCTA CAATGACTTT 
GGCACACAGG CCCATACTGG ACAGCTGCAG TCCATTCGAA GAGTCCAGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

First mutation in the ?A2-crystallin encoding gene is associated with small lenses and age-related cataracts.
Investigative ophthalmology & visual science52(5)2571-6(2011 Apr)
Puk O,Ahmad N,Wagner S,Hrab? de Angelis M,Graw J

Energetics of domain-domain interactions and entropy driven association of beta-crystallins.
Biochemistry43(2)415-24(2004 Jan)
Sergeev YV,Hejtmancik JF,Wingfield PT

Identification of the human beta A2 crystallin gene (CRYBA2): localization of the gene on human chromosome 2 and of the homologous gene on mouse chromosome 1.
Genomics28(3)543-8(1995 Aug)
Hulsebos TJ,Cerosaletti KM,Fournier RE,Sinke RJ,Rocchi M,Marzella R,Jenkins NA,Gilbert DJ,Copeland NG