Klhl12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klhl12
Entrez Gene ID	266772
Full Name	kelch-like family member 12
Synonyms	C3ip1
General protein information	Preferred Names kelch-like family member 12 Names kelch-like protein 12 CUL3-interacting protein 1 kelch-like 12 kelch-like protein C3IP1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13
Summary	contains several kelch domains [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249845.1	XP_006249907.1	kelch-like protein 12 isoform X2
XM_017598679.1	XP_017454168.1	kelch-like protein 12 isoform X2
NM_153730.2	NP_714952.1	kelch-like protein 12
XM_006249843.3	XP_006249905.1	kelch-like protein 12 isoform X1
XM_006249844.3	XP_006249906.1	kelch-like protein 12 isoform X1
XM_006249846.3	XP_006249908.1	kelch-like protein 12 isoform X3
XM_008769540.2	XP_008767762.1	kelch-like protein 12 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KLHL12	NP_067646.1
Canis lupus familiaris (dog)	KLHL12	XP_537119.2
Gallus gallus (chicken)	LOC101748977	XP_004934891.1
Pan troglodytes (chimpanzee)	KLHL12	XP_514106.3
Rattus norvegicus (Norway rat)	Klhl12	NP_714952.1
Danio rerio (zebrafish)	klhl12	NP_001007329.1
Bos taurus (cattle)	KLHL12	NP_001070593.1
	KLHL12	XP_002802018.1
Mus musculus (house mouse)	Klhl12	NP_694768.1
Xenopus tropicalis (tropical clawed frog)	klhl12	NP_001106569.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of the CUL3?Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
McGourty CA, Akopian D, Walsh C, Gorur A, Werner A, Schekman R, Bautista D, Rape M
Cell167(2)525-538.e14(2016 Oct)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report.
Butkinaree C, Guo L, Ramkhelawon B, Wanschel A, Brodsky JL, Moore KJ, Fisher EA
Arteriosclerosis, thrombosis, and vascular biology34(2)251-4(2014 Feb)

Ubiquitin-dependent regulation of COPII coat size and function.
Jin L, Pahuja KB, Wickliffe KE, Gorur A, Baumg?rtel C, Schekman R, Rape M
Nature482(7386)495-500(2012 Feb)

Nucleoredoxin sustains Wnt/?-catenin signaling by retaining a pool of inactive dishevelled protein.
Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H
Current biology : CB20(21)1945-52(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KLHL12 plays a critical role in facilitating the endoplasmic reticulum exit and secretion of apoB100-VLDL particles, suggesting that KLHL12 modulation would influence plasma lipid levels.
Title: A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report.

The following Klhl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klhl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12650	NM_153730.2 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa12650	XM_006249845.1 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa12650	XM_017598679.1 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa35351	XM_008769540.2 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa35351	XM_006249846.3 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa35350	XM_006249843.3 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa35350	XM_006249844.3 Latest version!	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klhl12 ( NM_153730.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_714952.1 Rattus norvegicus kelch-like family member 12 (Klhl12), mRNA.

Price & Availability↑

CloneID	ORa12650
Clone ID Related Accession (Same CDS sequence)	XM_006249845.1 , XM_017598679.1 , NM_153730.2
Accession Version	NM_153730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ133126.1. On Aug 30, 2012 this sequence version replaced NM_153730.1. ##Evidence-Data-START## Transcript exon combination :: AJ133126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_714952.1
CDS	8..1714
Translation	MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKGKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	NM_153730.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153730.2

ORF Insert Sequence:

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_006249845.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249907.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa12650
Clone ID Related Accession (Same CDS sequence)	XM_006249845.1 , XM_017598679.1 , NM_153730.2
Accession Version	XM_006249845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249907.1
CDS	137..1843
Translation	MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKGKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_006249845.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249845.1

ORF Insert Sequence:

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_017598679.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454168.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa12650
Clone ID Related Accession (Same CDS sequence)	XM_006249845.1 , XM_017598679.1 , NM_153730.2
Accession Version	XM_017598679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454168.1
CDS	275..1981
Translation	MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKGKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_017598679.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598679.1

ORF Insert Sequence:

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGCTTTC AGAGAAGGGG AAGCCATATG TTGACATTCA AGGTTTAACT 
GCTTCTACAA TGGAAATCCT GTTGGACTTC GTGTATACAG AAACAGTACA TGTGACAGTG 
GAGAATGTTC AAGAACTGCT CCCTGCAGCC TGTCTTCTCC AGTTGAAAGG TGTAAAACAA 
GCCTGCTGTG AGTTCTTAGA AAGTCAGTTG GATCCATCTA ATTGCCTGGG TATCAGGGAT 
TTTGCTGAAA CTCACAATTG CGTTGACCTG ATGCAAGCAG CTGAGGTTTT TAGCCAGAAG 
CATTTTCCTG AAGTGGTACA GCACGAAGAG TTCATTCTTC TGAGTCAAGG AGAGGTGGAG 
AAGCTAATCA AGTGCGATGA GATTCAGGTG GATTCTGAAG AGCCAGTCTT TGAGGCTGTC 
ATCAACTGGG TGAAGCATGC CAAGAAGGAG CGAGAGGAGT CTTTGCCTGA CCTCCTGCAG 
TATGTTCGGA TGCCCCTGCT GACCCCTAGG TACATTACAG ATGTGATTGA TGCTGAGCCT 
TTTATCCGCT GTAGTTTACA GTGCAGGGAC CTAGTTGATG AAGCCAAGAA GTTTCACCTG 
AGGCCTGAAC TTCGGAGTCA GATGCAAGGA CCCAGGACAA GAGCCCGACT AGGAGCCAAT 
GAAGTGCTTC TGGTGGTTGG GGGCTTCGGA AGCCAGCAGT CTCCTATTGA TGTGGTAGAG 
AAATATGACC CCAAGACACA GGAGTGGAGC TTTTTGCCAA GTATCACTCG CAAGAGACGG 
TATGTGGCCT CGGTTTCCTT ACATGATCGG ATCTATGTGA TTGGTGGCTA TGATGGCCGT 
TCCCGCCTCA GTTCAGTGGA GTGTCTAGAC TACACAGCAG ATGAAGACGG AGTGTGGTAC 
TCTGTGGCCC CTATGAATGT GCGGCGAGGC CTTGCTGGAG CCACCACACT GGGAGATATG 
ATTTACGTTT CTGGAGGCTT TGATGGAAGC AGGCGTCATA CAAGTATGGA GCGGTATGAC 
CCAAACATTG ACCAGTGGAG TATGCTGGGA GATATGCAGA CAGCTCGAGA GGGCGCGGGA 
CTAGTGGTGG CCAGCGGCAT AATCTACTGT CTAGGAGGAT ATGATGGCTT GAATATATTA 
AATTCAGTTG AGAAATATGA TCCCCATACA GGACACTGGA CTAATGTTAC GCCTATGGCC 
ACCAAGCGTT CTGGTGCTGG AGTAGCCCTA CTGAATGACC ATATTTATGT GGTGGGAGGG 
TTCGATGGTA CAGCCCACCT TTCTTCTGTT GAAGCTTATA ACATTCGCAC GGATTCCTGG 
ACAACTGTCA CAAGTATGAC CACACCACGA TGCTATGTAG GGGCCACAGT GCTTCGAGGG 
AGACTCTATG CAATCGCAGG ATACGATGGG AATTCTCTGC TGAGCAGCAT TGAGTGTTAT 
GACCCTATCA TCGACAGCTG GGAAGTAGTG GCCTCCATGG GAACCCAGCG TTGTGACGCT 
GGTGTGTGTG TTCTCCGAGA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_006249846.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249908.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa35351
Clone ID Related Accession (Same CDS sequence)	XM_006249846.3 , XM_008769540.2
Accession Version	XM_006249846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249846.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCC TGTTGGACTT CGTGTATACA GAAACAGTAC ATGTGACAGT GGAGAATGTT 
CAAGAACTGC TCCCTGCAGC CTGTCTTCTC CAGTTGAAAG GTGTAAAACA AGCCTGCTGT 
GAGTTCTTAG AAAGTCAGTT GGATCCATCT AATTGCCTGG GTATCAGGGA TTTTGCTGAA 
ACTCACAATT GCGTTGACCT GATGCAAGCA GCTGAGGTTT TTAGCCAGAA GCATTTTCCT 
GAAGTGGTAC AGCACGAAGA GTTCATTCTT CTGAGTCAAG GAGAGGTGGA GAAGCTAATC 
AAGTGCGATG AGATTCAGGT GGATTCTGAA GAGCCAGTCT TTGAGGCTGT CATCAACTGG 
GTGAAGCATG CCAAGAAGGA GCGAGAGGAG TCTTTGCCTG ACCTCCTGCA GTATGTTCGG 
ATGCCCCTGC TGACCCCTAG GTACATTACA GATGTGATTG ATGCTGAGCC TTTTATCCGC 
TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT GAAGCCAAGA AGTTTCACCT GAGGCCTGAA 
CTTCGGAGTC AGATGCAAGG ACCCAGGACA AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT 
CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG TCTCCTATTG ATGTGGTAGA GAAATATGAC 
CCCAAGACAC AGGAGTGGAG CTTTTTGCCA AGTATCACTC GCAAGAGACG GTATGTGGCC 
TCGGTTTCCT TACATGATCG GATCTATGTG ATTGGTGGCT ATGATGGCCG TTCCCGCCTC 
AGTTCAGTGG AGTGTCTAGA CTACACAGCA GATGAAGACG GAGTGTGGTA CTCTGTGGCC 
CCTATGAATG TGCGGCGAGG CCTTGCTGGA GCCACCACAC TGGGAGATAT GATTTACGTT 
TCTGGAGGCT TTGATGGAAG CAGGCGTCAT ACAAGTATGG AGCGGTATGA CCCAAACATT 
GACCAGTGGA GTATGCTGGG AGATATGCAG ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG 
GCCAGCGGCA TAATCTACTG TCTAGGAGGA TATGATGGCT TGAATATATT AAATTCAGTT 
GAGAAATATG ATCCCCATAC AGGACACTGG ACTAATGTTA CGCCTATGGC CACCAAGCGT 
TCTGGTGCTG GAGTAGCCCT ACTGAATGAC CATATTTATG TGGTGGGAGG GTTCGATGGT 
ACAGCCCACC TTTCTTCTGT TGAAGCTTAT AACATTCGCA CGGATTCCTG GACAACTGTC 
ACAAGTATGA CCACACCACG ATGCTATGTA GGGGCCACAG TGCTTCGAGG GAGACTCTAT 
GCAATCGCAG GATACGATGG GAATTCTCTG CTGAGCAGCA TTGAGTGTTA TGACCCTATC 
ATCGACAGCT GGGAAGTAGT GGCCTCCATG GGAACCCAGC GTTGTGACGC TGGTGTGTGT 
GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249908.1
CDS	465..1922
Translation	MEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_006249846.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249846.3

ORF Insert Sequence:

ATGGAAATCC TGTTGGACTT CGTGTATACA GAAACAGTAC ATGTGACAGT GGAGAATGTT 
CAAGAACTGC TCCCTGCAGC CTGTCTTCTC CAGTTGAAAG GTGTAAAACA AGCCTGCTGT 
GAGTTCTTAG AAAGTCAGTT GGATCCATCT AATTGCCTGG GTATCAGGGA TTTTGCTGAA 
ACTCACAATT GCGTTGACCT GATGCAAGCA GCTGAGGTTT TTAGCCAGAA GCATTTTCCT 
GAAGTGGTAC AGCACGAAGA GTTCATTCTT CTGAGTCAAG GAGAGGTGGA GAAGCTAATC 
AAGTGCGATG AGATTCAGGT GGATTCTGAA GAGCCAGTCT TTGAGGCTGT CATCAACTGG 
GTGAAGCATG CCAAGAAGGA GCGAGAGGAG TCTTTGCCTG ACCTCCTGCA GTATGTTCGG 
ATGCCCCTGC TGACCCCTAG GTACATTACA GATGTGATTG ATGCTGAGCC TTTTATCCGC 
TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT GAAGCCAAGA AGTTTCACCT GAGGCCTGAA 
CTTCGGAGTC AGATGCAAGG ACCCAGGACA AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT 
CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG TCTCCTATTG ATGTGGTAGA GAAATATGAC 
CCCAAGACAC AGGAGTGGAG CTTTTTGCCA AGTATCACTC GCAAGAGACG GTATGTGGCC 
TCGGTTTCCT TACATGATCG GATCTATGTG ATTGGTGGCT ATGATGGCCG TTCCCGCCTC 
AGTTCAGTGG AGTGTCTAGA CTACACAGCA GATGAAGACG GAGTGTGGTA CTCTGTGGCC 
CCTATGAATG TGCGGCGAGG CCTTGCTGGA GCCACCACAC TGGGAGATAT GATTTACGTT 
TCTGGAGGCT TTGATGGAAG CAGGCGTCAT ACAAGTATGG AGCGGTATGA CCCAAACATT 
GACCAGTGGA GTATGCTGGG AGATATGCAG ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG 
GCCAGCGGCA TAATCTACTG TCTAGGAGGA TATGATGGCT TGAATATATT AAATTCAGTT 
GAGAAATATG ATCCCCATAC AGGACACTGG ACTAATGTTA CGCCTATGGC CACCAAGCGT 
TCTGGTGCTG GAGTAGCCCT ACTGAATGAC CATATTTATG TGGTGGGAGG GTTCGATGGT 
ACAGCCCACC TTTCTTCTGT TGAAGCTTAT AACATTCGCA CGGATTCCTG GACAACTGTC 
ACAAGTATGA CCACACCACG ATGCTATGTA GGGGCCACAG TGCTTCGAGG GAGACTCTAT 
GCAATCGCAG GATACGATGG GAATTCTCTG CTGAGCAGCA TTGAGTGTTA TGACCCTATC 
ATCGACAGCT GGGAAGTAGT GGCCTCCATG GGAACCCAGC GTTGTGACGC TGGTGTGTGT 
GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_008769540.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767762.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa35351
Clone ID Related Accession (Same CDS sequence)	XM_006249846.3 , XM_008769540.2
Accession Version	XM_008769540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCC TGTTGGACTT CGTGTATACA GAAACAGTAC ATGTGACAGT GGAGAATGTT 
CAAGAACTGC TCCCTGCAGC CTGTCTTCTC CAGTTGAAAG GTGTAAAACA AGCCTGCTGT 
GAGTTCTTAG AAAGTCAGTT GGATCCATCT AATTGCCTGG GTATCAGGGA TTTTGCTGAA 
ACTCACAATT GCGTTGACCT GATGCAAGCA GCTGAGGTTT TTAGCCAGAA GCATTTTCCT 
GAAGTGGTAC AGCACGAAGA GTTCATTCTT CTGAGTCAAG GAGAGGTGGA GAAGCTAATC 
AAGTGCGATG AGATTCAGGT GGATTCTGAA GAGCCAGTCT TTGAGGCTGT CATCAACTGG 
GTGAAGCATG CCAAGAAGGA GCGAGAGGAG TCTTTGCCTG ACCTCCTGCA GTATGTTCGG 
ATGCCCCTGC TGACCCCTAG GTACATTACA GATGTGATTG ATGCTGAGCC TTTTATCCGC 
TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT GAAGCCAAGA AGTTTCACCT GAGGCCTGAA 
CTTCGGAGTC AGATGCAAGG ACCCAGGACA AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT 
CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG TCTCCTATTG ATGTGGTAGA GAAATATGAC 
CCCAAGACAC AGGAGTGGAG CTTTTTGCCA AGTATCACTC GCAAGAGACG GTATGTGGCC 
TCGGTTTCCT TACATGATCG GATCTATGTG ATTGGTGGCT ATGATGGCCG TTCCCGCCTC 
AGTTCAGTGG AGTGTCTAGA CTACACAGCA GATGAAGACG GAGTGTGGTA CTCTGTGGCC 
CCTATGAATG TGCGGCGAGG CCTTGCTGGA GCCACCACAC TGGGAGATAT GATTTACGTT 
TCTGGAGGCT TTGATGGAAG CAGGCGTCAT ACAAGTATGG AGCGGTATGA CCCAAACATT 
GACCAGTGGA GTATGCTGGG AGATATGCAG ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG 
GCCAGCGGCA TAATCTACTG TCTAGGAGGA TATGATGGCT TGAATATATT AAATTCAGTT 
GAGAAATATG ATCCCCATAC AGGACACTGG ACTAATGTTA CGCCTATGGC CACCAAGCGT 
TCTGGTGCTG GAGTAGCCCT ACTGAATGAC CATATTTATG TGGTGGGAGG GTTCGATGGT 
ACAGCCCACC TTTCTTCTGT TGAAGCTTAT AACATTCGCA CGGATTCCTG GACAACTGTC 
ACAAGTATGA CCACACCACG ATGCTATGTA GGGGCCACAG TGCTTCGAGG GAGACTCTAT 
GCAATCGCAG GATACGATGG GAATTCTCTG CTGAGCAGCA TTGAGTGTTA TGACCCTATC 
ATCGACAGCT GGGAAGTAGT GGCCTCCATG GGAACCCAGC GTTGTGACGC TGGTGTGTGT 
GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767762.1
CDS	820..2277
Translation	MEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_008769540.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769540.2

ORF Insert Sequence:

ATGGAAATCC TGTTGGACTT CGTGTATACA GAAACAGTAC ATGTGACAGT GGAGAATGTT 
CAAGAACTGC TCCCTGCAGC CTGTCTTCTC CAGTTGAAAG GTGTAAAACA AGCCTGCTGT 
GAGTTCTTAG AAAGTCAGTT GGATCCATCT AATTGCCTGG GTATCAGGGA TTTTGCTGAA 
ACTCACAATT GCGTTGACCT GATGCAAGCA GCTGAGGTTT TTAGCCAGAA GCATTTTCCT 
GAAGTGGTAC AGCACGAAGA GTTCATTCTT CTGAGTCAAG GAGAGGTGGA GAAGCTAATC 
AAGTGCGATG AGATTCAGGT GGATTCTGAA GAGCCAGTCT TTGAGGCTGT CATCAACTGG 
GTGAAGCATG CCAAGAAGGA GCGAGAGGAG TCTTTGCCTG ACCTCCTGCA GTATGTTCGG 
ATGCCCCTGC TGACCCCTAG GTACATTACA GATGTGATTG ATGCTGAGCC TTTTATCCGC 
TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT GAAGCCAAGA AGTTTCACCT GAGGCCTGAA 
CTTCGGAGTC AGATGCAAGG ACCCAGGACA AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT 
CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG TCTCCTATTG ATGTGGTAGA GAAATATGAC 
CCCAAGACAC AGGAGTGGAG CTTTTTGCCA AGTATCACTC GCAAGAGACG GTATGTGGCC 
TCGGTTTCCT TACATGATCG GATCTATGTG ATTGGTGGCT ATGATGGCCG TTCCCGCCTC 
AGTTCAGTGG AGTGTCTAGA CTACACAGCA GATGAAGACG GAGTGTGGTA CTCTGTGGCC 
CCTATGAATG TGCGGCGAGG CCTTGCTGGA GCCACCACAC TGGGAGATAT GATTTACGTT 
TCTGGAGGCT TTGATGGAAG CAGGCGTCAT ACAAGTATGG AGCGGTATGA CCCAAACATT 
GACCAGTGGA GTATGCTGGG AGATATGCAG ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG 
GCCAGCGGCA TAATCTACTG TCTAGGAGGA TATGATGGCT TGAATATATT AAATTCAGTT 
GAGAAATATG ATCCCCATAC AGGACACTGG ACTAATGTTA CGCCTATGGC CACCAAGCGT 
TCTGGTGCTG GAGTAGCCCT ACTGAATGAC CATATTTATG TGGTGGGAGG GTTCGATGGT 
ACAGCCCACC TTTCTTCTGT TGAAGCTTAT AACATTCGCA CGGATTCCTG GACAACTGTC 
ACAAGTATGA CCACACCACG ATGCTATGTA GGGGCCACAG TGCTTCGAGG GAGACTCTAT 
GCAATCGCAG GATACGATGG GAATTCTCTG CTGAGCAGCA TTGAGTGTTA TGACCCTATC 
ATCGACAGCT GGGAAGTAGT GGCCTCCATG GGAACCCAGC GTTGTGACGC TGGTGTGTGT 
GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_006249843.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249905.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35350
Clone ID Related Accession (Same CDS sequence)	XM_006249843.3 , XM_006249844.3
Accession Version	XM_006249843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGGTAAA TGCGAGTCTT TGCTATCTTT CAGAGAAGGG GAAGCCATAT 
GTTGACATTC AAGGTTTAAC TGCTTCTACA ATGGAAATCC TGTTGGACTT CGTGTATACA 
GAAACAGTAC ATGTGACAGT GGAGAATGTT CAAGAACTGC TCCCTGCAGC CTGTCTTCTC 
CAGTTGAAAG GTGTAAAACA AGCCTGCTGT GAGTTCTTAG AAAGTCAGTT GGATCCATCT 
AATTGCCTGG GTATCAGGGA TTTTGCTGAA ACTCACAATT GCGTTGACCT GATGCAAGCA 
GCTGAGGTTT TTAGCCAGAA GCATTTTCCT GAAGTGGTAC AGCACGAAGA GTTCATTCTT 
CTGAGTCAAG GAGAGGTGGA GAAGCTAATC AAGTGCGATG AGATTCAGGT GGATTCTGAA 
GAGCCAGTCT TTGAGGCTGT CATCAACTGG GTGAAGCATG CCAAGAAGGA GCGAGAGGAG 
TCTTTGCCTG ACCTCCTGCA GTATGTTCGG ATGCCCCTGC TGACCCCTAG GTACATTACA 
GATGTGATTG ATGCTGAGCC TTTTATCCGC TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT 
GAAGCCAAGA AGTTTCACCT GAGGCCTGAA CTTCGGAGTC AGATGCAAGG ACCCAGGACA 
AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG 
TCTCCTATTG ATGTGGTAGA GAAATATGAC CCCAAGACAC AGGAGTGGAG CTTTTTGCCA 
AGTATCACTC GCAAGAGACG GTATGTGGCC TCGGTTTCCT TACATGATCG GATCTATGTG 
ATTGGTGGCT ATGATGGCCG TTCCCGCCTC AGTTCAGTGG AGTGTCTAGA CTACACAGCA 
GATGAAGACG GAGTGTGGTA CTCTGTGGCC CCTATGAATG TGCGGCGAGG CCTTGCTGGA 
GCCACCACAC TGGGAGATAT GATTTACGTT TCTGGAGGCT TTGATGGAAG CAGGCGTCAT 
ACAAGTATGG AGCGGTATGA CCCAAACATT GACCAGTGGA GTATGCTGGG AGATATGCAG 
ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG GCCAGCGGCA TAATCTACTG TCTAGGAGGA 
TATGATGGCT TGAATATATT AAATTCAGTT GAGAAATATG ATCCCCATAC AGGACACTGG 
ACTAATGTTA CGCCTATGGC CACCAAGCGT TCTGGTGCTG GAGTAGCCCT ACTGAATGAC 
CATATTTATG TGGTGGGAGG GTTCGATGGT ACAGCCCACC TTTCTTCTGT TGAAGCTTAT 
AACATTCGCA CGGATTCCTG GACAACTGTC ACAAGTATGA CCACACCACG ATGCTATGTA 
GGGGCCACAG TGCTTCGAGG GAGACTCTAT GCAATCGCAG GATACGATGG GAATTCTCTG 
CTGAGCAGCA TTGAGTGTTA TGACCCTATC ATCGACAGCT GGGAAGTAGT GGCCTCCATG 
GGAACCCAGC GTTGTGACGC TGGTGTGTGT GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249905.1
CDS	132..1859
Translation	MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSEVNASLCYLSEKGKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_006249843.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249843.3

ORF Insert Sequence:

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGGTAAA TGCGAGTCTT TGCTATCTTT CAGAGAAGGG GAAGCCATAT 
GTTGACATTC AAGGTTTAAC TGCTTCTACA ATGGAAATCC TGTTGGACTT CGTGTATACA 
GAAACAGTAC ATGTGACAGT GGAGAATGTT CAAGAACTGC TCCCTGCAGC CTGTCTTCTC 
CAGTTGAAAG GTGTAAAACA AGCCTGCTGT GAGTTCTTAG AAAGTCAGTT GGATCCATCT 
AATTGCCTGG GTATCAGGGA TTTTGCTGAA ACTCACAATT GCGTTGACCT GATGCAAGCA 
GCTGAGGTTT TTAGCCAGAA GCATTTTCCT GAAGTGGTAC AGCACGAAGA GTTCATTCTT 
CTGAGTCAAG GAGAGGTGGA GAAGCTAATC AAGTGCGATG AGATTCAGGT GGATTCTGAA 
GAGCCAGTCT TTGAGGCTGT CATCAACTGG GTGAAGCATG CCAAGAAGGA GCGAGAGGAG 
TCTTTGCCTG ACCTCCTGCA GTATGTTCGG ATGCCCCTGC TGACCCCTAG GTACATTACA 
GATGTGATTG ATGCTGAGCC TTTTATCCGC TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT 
GAAGCCAAGA AGTTTCACCT GAGGCCTGAA CTTCGGAGTC AGATGCAAGG ACCCAGGACA 
AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG 
TCTCCTATTG ATGTGGTAGA GAAATATGAC CCCAAGACAC AGGAGTGGAG CTTTTTGCCA 
AGTATCACTC GCAAGAGACG GTATGTGGCC TCGGTTTCCT TACATGATCG GATCTATGTG 
ATTGGTGGCT ATGATGGCCG TTCCCGCCTC AGTTCAGTGG AGTGTCTAGA CTACACAGCA 
GATGAAGACG GAGTGTGGTA CTCTGTGGCC CCTATGAATG TGCGGCGAGG CCTTGCTGGA 
GCCACCACAC TGGGAGATAT GATTTACGTT TCTGGAGGCT TTGATGGAAG CAGGCGTCAT 
ACAAGTATGG AGCGGTATGA CCCAAACATT GACCAGTGGA GTATGCTGGG AGATATGCAG 
ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG GCCAGCGGCA TAATCTACTG TCTAGGAGGA 
TATGATGGCT TGAATATATT AAATTCAGTT GAGAAATATG ATCCCCATAC AGGACACTGG 
ACTAATGTTA CGCCTATGGC CACCAAGCGT TCTGGTGCTG GAGTAGCCCT ACTGAATGAC 
CATATTTATG TGGTGGGAGG GTTCGATGGT ACAGCCCACC TTTCTTCTGT TGAAGCTTAT 
AACATTCGCA CGGATTCCTG GACAACTGTC ACAAGTATGA CCACACCACG ATGCTATGTA 
GGGGCCACAG TGCTTCGAGG GAGACTCTAT GCAATCGCAG GATACGATGG GAATTCTCTG 
CTGAGCAGCA TTGAGTGTTA TGACCCTATC ATCGACAGCT GGGAAGTAGT GGCCTCCATG 
GGAACCCAGC GTTGTGACGC TGGTGTGTGT GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl12 ( XM_006249844.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249906.1 Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X2, mRNA.

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CloneID	ORa35350
Clone ID Related Accession (Same CDS sequence)	XM_006249843.3 , XM_006249844.3
Accession Version	XM_006249844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kelch-like protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGGTAAA TGCGAGTCTT TGCTATCTTT CAGAGAAGGG GAAGCCATAT 
GTTGACATTC AAGGTTTAAC TGCTTCTACA ATGGAAATCC TGTTGGACTT CGTGTATACA 
GAAACAGTAC ATGTGACAGT GGAGAATGTT CAAGAACTGC TCCCTGCAGC CTGTCTTCTC 
CAGTTGAAAG GTGTAAAACA AGCCTGCTGT GAGTTCTTAG AAAGTCAGTT GGATCCATCT 
AATTGCCTGG GTATCAGGGA TTTTGCTGAA ACTCACAATT GCGTTGACCT GATGCAAGCA 
GCTGAGGTTT TTAGCCAGAA GCATTTTCCT GAAGTGGTAC AGCACGAAGA GTTCATTCTT 
CTGAGTCAAG GAGAGGTGGA GAAGCTAATC AAGTGCGATG AGATTCAGGT GGATTCTGAA 
GAGCCAGTCT TTGAGGCTGT CATCAACTGG GTGAAGCATG CCAAGAAGGA GCGAGAGGAG 
TCTTTGCCTG ACCTCCTGCA GTATGTTCGG ATGCCCCTGC TGACCCCTAG GTACATTACA 
GATGTGATTG ATGCTGAGCC TTTTATCCGC TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT 
GAAGCCAAGA AGTTTCACCT GAGGCCTGAA CTTCGGAGTC AGATGCAAGG ACCCAGGACA 
AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG 
TCTCCTATTG ATGTGGTAGA GAAATATGAC CCCAAGACAC AGGAGTGGAG CTTTTTGCCA 
AGTATCACTC GCAAGAGACG GTATGTGGCC TCGGTTTCCT TACATGATCG GATCTATGTG 
ATTGGTGGCT ATGATGGCCG TTCCCGCCTC AGTTCAGTGG AGTGTCTAGA CTACACAGCA 
GATGAAGACG GAGTGTGGTA CTCTGTGGCC CCTATGAATG TGCGGCGAGG CCTTGCTGGA 
GCCACCACAC TGGGAGATAT GATTTACGTT TCTGGAGGCT TTGATGGAAG CAGGCGTCAT 
ACAAGTATGG AGCGGTATGA CCCAAACATT GACCAGTGGA GTATGCTGGG AGATATGCAG 
ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG GCCAGCGGCA TAATCTACTG TCTAGGAGGA 
TATGATGGCT TGAATATATT AAATTCAGTT GAGAAATATG ATCCCCATAC AGGACACTGG 
ACTAATGTTA CGCCTATGGC CACCAAGCGT TCTGGTGCTG GAGTAGCCCT ACTGAATGAC 
CATATTTATG TGGTGGGAGG GTTCGATGGT ACAGCCCACC TTTCTTCTGT TGAAGCTTAT 
AACATTCGCA CGGATTCCTG GACAACTGTC ACAAGTATGA CCACACCACG ATGCTATGTA 
GGGGCCACAG TGCTTCGAGG GAGACTCTAT GCAATCGCAG GATACGATGG GAATTCTCTG 
CTGAGCAGCA TTGAGTGTTA TGACCCTATC ATCGACAGCT GGGAAGTAGT GGCCTCCATG 
GGAACCCAGC GTTGTGACGC TGGTGTGTGT GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249906.1
CDS	406..2133
Translation	MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSEVNASLCYLSEKGKPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKEREESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK

Target ORF information:

RefSeq Version	XM_006249844.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kelch-like family member 12 (Klhl12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249844.3

ORF Insert Sequence:

ATGGGCGGCA TTATGGCCCC TAAAGACATA ATGACAAATA CTCACGCCAA GTCCATCCTC 
AACTCAATGA ACTCCCTCAG GAAGAGCAAT ACCCTCTGTG ACGTGACCTT GAGAGTGGAG 
CAGAAAGACT TCCCTGCCCA CCGGATTGTG CTGGCTGCCT GCAGCGATTA TTTCTGTGCC 
ATGTTCACTA GTGAGGTAAA TGCGAGTCTT TGCTATCTTT CAGAGAAGGG GAAGCCATAT 
GTTGACATTC AAGGTTTAAC TGCTTCTACA ATGGAAATCC TGTTGGACTT CGTGTATACA 
GAAACAGTAC ATGTGACAGT GGAGAATGTT CAAGAACTGC TCCCTGCAGC CTGTCTTCTC 
CAGTTGAAAG GTGTAAAACA AGCCTGCTGT GAGTTCTTAG AAAGTCAGTT GGATCCATCT 
AATTGCCTGG GTATCAGGGA TTTTGCTGAA ACTCACAATT GCGTTGACCT GATGCAAGCA 
GCTGAGGTTT TTAGCCAGAA GCATTTTCCT GAAGTGGTAC AGCACGAAGA GTTCATTCTT 
CTGAGTCAAG GAGAGGTGGA GAAGCTAATC AAGTGCGATG AGATTCAGGT GGATTCTGAA 
GAGCCAGTCT TTGAGGCTGT CATCAACTGG GTGAAGCATG CCAAGAAGGA GCGAGAGGAG 
TCTTTGCCTG ACCTCCTGCA GTATGTTCGG ATGCCCCTGC TGACCCCTAG GTACATTACA 
GATGTGATTG ATGCTGAGCC TTTTATCCGC TGTAGTTTAC AGTGCAGGGA CCTAGTTGAT 
GAAGCCAAGA AGTTTCACCT GAGGCCTGAA CTTCGGAGTC AGATGCAAGG ACCCAGGACA 
AGAGCCCGAC TAGGAGCCAA TGAAGTGCTT CTGGTGGTTG GGGGCTTCGG AAGCCAGCAG 
TCTCCTATTG ATGTGGTAGA GAAATATGAC CCCAAGACAC AGGAGTGGAG CTTTTTGCCA 
AGTATCACTC GCAAGAGACG GTATGTGGCC TCGGTTTCCT TACATGATCG GATCTATGTG 
ATTGGTGGCT ATGATGGCCG TTCCCGCCTC AGTTCAGTGG AGTGTCTAGA CTACACAGCA 
GATGAAGACG GAGTGTGGTA CTCTGTGGCC CCTATGAATG TGCGGCGAGG CCTTGCTGGA 
GCCACCACAC TGGGAGATAT GATTTACGTT TCTGGAGGCT TTGATGGAAG CAGGCGTCAT 
ACAAGTATGG AGCGGTATGA CCCAAACATT GACCAGTGGA GTATGCTGGG AGATATGCAG 
ACAGCTCGAG AGGGCGCGGG ACTAGTGGTG GCCAGCGGCA TAATCTACTG TCTAGGAGGA 
TATGATGGCT TGAATATATT AAATTCAGTT GAGAAATATG ATCCCCATAC AGGACACTGG 
ACTAATGTTA CGCCTATGGC CACCAAGCGT TCTGGTGCTG GAGTAGCCCT ACTGAATGAC 
CATATTTATG TGGTGGGAGG GTTCGATGGT ACAGCCCACC TTTCTTCTGT TGAAGCTTAT 
AACATTCGCA CGGATTCCTG GACAACTGTC ACAAGTATGA CCACACCACG ATGCTATGTA 
GGGGCCACAG TGCTTCGAGG GAGACTCTAT GCAATCGCAG GATACGATGG GAATTCTCTG 
CTGAGCAGCA TTGAGTGTTA TGACCCTATC ATCGACAGCT GGGAAGTAGT GGCCTCCATG 
GGAACCCAGC GTTGTGACGC TGGTGTGTGT GTTCTCCGAG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of the CUL3?Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
Cell167(2)525-538.e14(2016 Oct)
McGourty CA,Akopian D,Walsh C,Gorur A,Werner A,Schekman R,Bautista D,Rape M

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report.
Arteriosclerosis, thrombosis, and vascular biology34(2)251-4(2014 Feb)
Butkinaree C,Guo L,Ramkhelawon B,Wanschel A,Brodsky JL,Moore KJ,Fisher EA

Ubiquitin-dependent regulation of COPII coat size and function.
Nature482(7386)495-500(2012 Feb)
Jin L,Pahuja KB,Wickliffe KE,Gorur A,Baumg?rtel C,Schekman R,Rape M

Nucleoredoxin sustains Wnt/?-catenin signaling by retaining a pool of inactive dishevelled protein.
Current biology : CB20(21)1945-52(2010 Nov)
Funato Y,Terabayashi T,Sakamoto R,Okuzaki D,Ichise H,Nojima H,Yoshida N,Miki H

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Klhl12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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