Prkar1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkar1a
Entrez Gene ID	25725
Full Name	protein kinase cAMP-dependent type 1 regulatory subunit alpha
Synonyms	RIIA
General protein information	Preferred Names protein kinase cAMP-dependent type 1 regulatory subunit alpha Names cAMP-dependent protein kinase type I-alpha regulatory subunit protein kinase, cAMP-dependent, regulatory subunit type I alpha protein kinase, cAMP-dependent, regulatory, type I, alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	regulatory subunit of cAMP-dependent protein kinase (PKA); negatively regulates meiosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013181.1	NP_037313.1	cAMP-dependent protein kinase type I-alpha regulatory subunit
XM_008768311.1	XP_008766533.1	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
XM_017597047.1	XP_017452536.1	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006448	XP_001688871.1
Danio rerio (zebrafish)	prkar1ab	NP_001017732.1
Drosophila melanogaster (fruit fly)	Pka-R1	NP_730573.2
Homo sapiens (human)	PRKAR1A	NP_997637.1
Pan troglodytes (chimpanzee)	PRKAR1A	XP_003315746.1
	LOC719021	XP_001112547.2
Canis lupus familiaris (dog)	PRKAR1A	XP_005624335.1
Bos taurus (cattle)	PRKAR1A	NP_001069826.1
Gallus gallus (chicken)	PRKAR1A	NP_001007846.1
Danio rerio (zebrafish)	prkar1aa	NP_001009989.1
Mus musculus (house mouse)	Prkar1a	NP_068680.1
Rattus norvegicus (Norway rat)	Prkar1a	NP_037313.1
Xenopus tropicalis (tropical clawed frog)	prkar1a	NP_001025530.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.
Gilbert ML, Yang L, Su T, McKnight GS
American journal of physiology. Renal physiology308(6)F627-38(2015 Mar)

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Burgers PP, van der Heyden MA, Kok B, Heck AJ, Scholten A
Biochemistry54(1)11-21(2015 Jan)

Cytoplasmic anchoring of cAMP-dependent protein kinase (PKA) by A-kinase anchor proteins (AKAPs) is required for meiotic arrest of porcine full-grown and growing oocytes.
Nishimura T, Fujii W, Sugiura K, Naito K
Biology of reproduction90(3)58(2014 Mar)

Interactions between the regulatory subunit of type I protein kinase A and p90 ribosomal S6 kinase1 regulate cardiomyocyte apoptosis.
Gao X, Lin B, Sadayappan S, Patel TB
Molecular pharmacology85(2)357-67(2014 Feb)

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Gr?nborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, Jahn O, Brose N, Herzog E
The EMBO journal33(2)157-70(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

role for PKA signaling in both short- and long-term regulation of AQP2, characterizing a novel mouse model of diabetes insipidus
Title: Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.

hypoxia/reoxygenation (H/R)-mediated decrease in PKARIalpha protein levels leads to activation of RSK1, which via phosphorylation of NHE1 induces cardiomyocyte apoptosis.
Title: Interactions between the regulatory subunit of type I protein kinase A and p90 ribosomal S6 kinase1 regulate cardiomyocyte apoptosis.

a key role for AKAP-targeted PKA in control of heart rate and contractile function
Title: Disruption of protein kinase A localization using a trans-activator of transcription (TAT)-conjugated A-kinase-anchoring peptide reduces cardiac function.

EPAC & PKA modulate both distinct & common exocytic steps to potentiate insulin exocytosis. EPAC mobilizes SGs to fuse at the PM. PKA & EPAC synergistically modulate SG-SG fusions underlying compound & sequential exocytoses.
Title: Activation of exchange protein directly activated by cyclic adenosine monophosphate and protein kinase A regulate common and distinct steps in promoting plasma membrane exocytic and granule-to-granule fusions in rat islet beta cells.

dystrobrevin is a scaffold protein that may play a role in different cellular processes involving PKA signaling
Title: Association of dystrobrevin and regulatory subunit of protein kinase A: a new role for dystrobrevin as a scaffold for signaling proteins.

The following Prkar1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkar1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02237	NM_013181.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit alpha (Prkar1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa02237	XM_017597047.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa02237	XM_008768311.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkar1a ( NM_013181.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037313.1 Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit alpha (Prkar1a), mRNA.

Price & Availability↑

CloneID	ORa02237
Clone ID Related Accession (Same CDS sequence)	NM_013181.1 , XM_008768311.1 , XM_017597047.1
Accession Version	NM_013181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M17086.1. On Apr 19, 2005 this sequence version replaced XM_579380.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M17086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037313.1
CDS	96..1241
Translation	MASGSMAASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEARQIQSLQKSGIRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGTAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	NM_013181.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit alpha (Prkar1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013181.1

ORF Insert Sequence:

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkar1a ( XM_008768311.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766533.1 Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa02237
Clone ID Related Accession (Same CDS sequence)	NM_013181.1 , XM_008768311.1 , XM_017597047.1
Accession Version	XM_008768311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766533.1
CDS	152..1297
Translation	MASGSMAASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEARQIQSLQKSGIRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGTAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_008768311.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768311.1

ORF Insert Sequence:

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkar1a ( XM_017597047.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452536.1 Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02237
Clone ID Related Accession (Same CDS sequence)	NM_013181.1 , XM_008768311.1 , XM_017597047.1
Accession Version	XM_017597047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452536.1
CDS	30..1175
Translation	MASGSMAASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEARQIQSLQKSGIRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGTAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_017597047.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit alpha (Prkar1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597047.1

ORF Insert Sequence:

ATGGCGTCTG GCAGTATGGC AGCCAGTGAG GAAGAGCGGA GTCTCCGGGA ATGCGAGCTC 
TATGTGCAGA AGCACAACAT CCAGGCCCTG CTGAAGGACT CCATCGTGCA GCTGTGCACT 
GCTCGGCCCG AGAGGCCCAT GGCATTCCTT CGGGAATACT TTGAGAGGCT GGAGAAGGAG 
GAGGCAAGAC AGATTCAGAG CCTACAGAAA TCCGGCATTC GTACAGACTC GAGGGAGGAT 
GAGATCTCTC CTCCACCCCC AAATCCGGTG GTGAAGGGCC GTCGGCGCCG CGGTGCTATC 
AGCGCTGAGG TTTACACTGA GGAAGATGCT GCGTCCTACG TTAGAAAGGT TATACCAAAA 
GATTATAAGA CAATGGCTGC TTTAGCCAAG GCCATCGAAA AGAACGTGTT ATTTTCACAC 
CTTGATGACA ATGAGAGAAG TGACATTTTT GATGCAATGT TTCCAGTCTC CTTTATTGCT 
GGAGAGACAG TTATTCAGCA AGGGGATGAA GGGGATAACT TCTATGTGAT TGATCAAGGA 
GAAATGGATG TGTATGTCAA TAATGAATGG GCAACCAGTG TTGGGGAAGG AGGGAGCTTT 
GGAGAGCTGG CTTTGATTTA CGGAACACCC AGAGCAGCCA CTGTCAAAGC AAAGACAAAC 
GTGAAACTGT GGGGCATTGA CCGAGACAGC TACCGGAGAA TCCTCATGGG AAGCACTCTG 
CGAAAGAGGA AGATGTATGA AGAATTTCTT AGTAAAGTGT CTATTTTAGA GTCTCTGGAC 
AAGTGGGAGC GTCTCACAGT AGCTGATGCA CTGGAGCCTG TCCAGTTTGA AGATGGACAG 
AAGATTGTGG TGCAAGGAGA ACCGGGGGAT GAGTTCTTCA TTATTTTAGA GGGCACGGCC 
GCTGTGCTGC AGCGTCGGTC AGAAAACGAA GAGTTTGTTG AAGTGGGACG CCTGGGGCCT 
TCTGACTATT TTGGTGAAAT TGCCCTGCTG ATGAATCGTC CTCGGGCTGC CACTGTGGTT 
GCGCGTGGCC CTTTGAAGTG CGTTAAGTTG GACCGACCTC GGTTTGAACG CGTCCTTGGC 
CCATGCTCAG ATATCCTCAA GCGAAACATC CAGCAGTACA ACAGCTTTGT GTCACTGTCC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.
American journal of physiology. Renal physiology308(6)F627-38(2015 Mar)
Gilbert ML,Yang L,Su T,McKnight GS

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Biochemistry54(1)11-21(2015 Jan)
Burgers PP,van der Heyden MA,Kok B,Heck AJ,Scholten A

Cytoplasmic anchoring of cAMP-dependent protein kinase (PKA) by A-kinase anchor proteins (AKAPs) is required for meiotic arrest of porcine full-grown and growing oocytes.
Biology of reproduction90(3)58(2014 Mar)
Nishimura T,Fujii W,Sugiura K,Naito K

Interactions between the regulatory subunit of type I protein kinase A and p90 ribosomal S6 kinase1 regulate cardiomyocyte apoptosis.
Molecular pharmacology85(2)357-67(2014 Feb)
Gao X,Lin B,Sadayappan S,Patel TB

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
The EMBO journal33(2)157-70(2014 Jan)
Biesemann C,Gr?nborg M,Luquet E,Wichert SP,Bernard V,Bungers SR,Cooper B,Varoqueaux F,Li L,Byrne JA,Urlaub H,Jahn O,Brose N,Herzog E

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Prkar1a cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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