Syt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syt1
Entrez Gene ID	25716
Full Name	synaptotagmin 1
Synonyms	P65
General protein information	Preferred Names synaptotagmin 1 Names synaptotagmin-1 synaptotagmin I sytI
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q21
Summary	integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594679.1	XP_017450168.1	synaptotagmin-1 isoform X1
XM_008765353.2	XP_008763575.2	synaptotagmin-1 isoform X2
NM_001033680.2	NP_001028852.2	synaptotagmin-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYT1	NP_001129278.1
Pan troglodytes (chimpanzee)	SYT1	XP_001162865.1
Canis lupus familiaris (dog)	SYT1	XP_539696.2
Bos taurus (cattle)	SYT1	NP_776617.1
Rattus norvegicus (Norway rat)	Syt1	NP_001028852.2
Gallus gallus (chicken)	SYT1	NP_990502.1
Xenopus tropicalis (tropical clawed frog)	syt1	XP_004913017.1
	SYT1	XP_001084494.2
Caenorhabditis elegans (roundworm)	snt-1	NP_495394.3
Mus musculus (house mouse)	Syt1	NP_001239270.1
Drosophila melanogaster (fruit fly)	Syt1	NP_722839.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007942	XP_317535.4

Gene Ontology
Bibliography

Related articles in PubMed

The calcium sensor synaptotagmin 1 is expressed and regulated in hippocampal postsynaptic spines.
Hussain S, Egbenya DL, Lai YC, Dosa ZJ, S?rensen JB, Anderson AE, Davanger S
Hippocampus27(11)1168-1177(2017 Nov)

Membrane protein Nav1.7 contributes to the persistent post-surgical pain regulated by p-p65 in dorsal root ganglion (DRG) of SMIR rats model.
Li Z, Li Y, Cao J, Han X, Cai W, Zang W, Xu J, Zhang W
BMC anesthesiology17(1)150(2017 Nov)

G?? directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes.
Zurawski Z, Page B, Chicka MC, Brindley RL, Wells CA, Preininger AM, Hyde K, Gilbert JA, Cruz-Rodriguez O, Currie KPM, Chapman ER, Alford S, Hamm HE
The Journal of biological chemistry292(29)12165-12177(2017 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This work has shed new light on the molecular mechanism for Rab3 and synaptotamin regulation of neurotransmitter release.
Title: Rab3A Inhibition of Ca

Data suggest that GTP-binding protein beta/gamma (Gb/g) heterodimers interact specifically with lipid-embedded neuronal t-SNARE proteins syntaxin 1A and SNAP-25B (synaptosomal-associated protein 25B); this binding is competitive with synaptotagmin 1 for binding sites on the t-SNARE proteins; Gb/g inhibits Ca2+/synaptotagmin 1-dependent membrane fusion.
Title: G?? directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes.

Rab3A-binding site on C2A domain of synaptotagmin I
Title: Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism.

After single synaptic vesicle fusion, syt1 acted as an essential determinant of synaptic vesicle endocytosis time course by delaying the kinetics of vesicle retrieval in response to increasing Ca(2+) levels.
Title: Synaptotagmin-1- and Synaptotagmin-7-Dependent Fusion Mechanisms Target Synaptic Vesicles to Kinetically Distinct Endocytic Pathways.

The authors show that synaptotagmin-1 (from Rattus norvegicus and expressed in Escherichia coli) binds to phosphatidylinositol 4,5-diphosphate via a polybasic lysine patch in the C2B domain, which may promote the priming or docking of synaptic vesicles.
Title: PtdInsP

The following Syt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13122	NM_001033680.2 Latest version!	Rattus norvegicus synaptotagmin 1 (Syt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa13122	XM_017594679.1 Latest version!	Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa61909	XM_008765353.2 Latest version!	Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syt1 ( NM_001033680.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028852.2 Rattus norvegicus synaptotagmin 1 (Syt1), mRNA.

Price & Availability↑

CloneID	ORa13122
Clone ID Related Accession (Same CDS sequence)	XM_017594679.1 , NM_001033680.2
Accession Version	NM_001033680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Rattus norvegicus(Norway rat)
Product	synaptotagmin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ617621.1, AA924659.1, CB731032.1, CO398869.1 and CB615786.1. On May 26, 2007 this sequence version replaced NM_001033680.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGC CCTTAAGGAT 
GACGATGCTG AAACCGGACT GACTGATGGA GAAGAAAAGG AAGAGCCCAA GGAAGAGGAG 
AAACTGGGAA AGCTCCAATA TTCACTGGAC TATGACTTCC AGAATAACCA GCTGTTGGTG 
GGAATCATCC AGGCTGCTGA ACTGCCCGCC CTGGACATGG GGGGTACATC CGATCCATAC 
GTCAAAGTCT TCCTGCTGCC TGACAAAAAG AAGAAATTTG AGACTAAAGT CCACCGGAAA 
ACCCTCAATC CAGTCTTCAA TGAACAATTT ACTTTCAAGG TGCCCTACTC GGAATTAGGT 
GGCAAAACCC TGGTGATGGC TGTGTATGAC TTTGATCGCT TCTCCAAGCA TGACATCATC 
GGAGAGTTCA AAGTTCCTAT GAACACCGTG GATTTTGGCC ATGTGACCGA GGAGTGGCGT 
GATCTCCAGA GCGCTGAGAA AGAAGAGCAA GAGAAACTGG GTGACATCTG CTTCTCCCTC 
CGCTACGTCC CTACTGCCGG CAAACTGACT GTTGTCATTC TGGAAGCCAA GAACCTGAAG 
AAGATGGATG TGGGTGGCTT ATCTGATCCC TACGTGAAGA TTCACCTGAT GCAGAACGGT 
AAGAGGCTGA AGAAGAAAAA GACGACGATT AAGAAGAACA CACTCAACCC CTACTACAAC 
GAGTCCTTCA GCTTTGAAGT TCCGTTCGAG CAAATCCAGA AAGTGCAAGT GGTGGTAACT 
GTTTTGGACT ATGACAAGAT TGGCAAGAAC GACGCCATCG GCAAAGTCTT CGTTGGTTAC 
AACAGCACTG GGGCGGAGCT GCGACACTGG TCAGACATGC TGGCCAACCC CCGGCGACCC 
ATCGCACAGT GGCACACTCT GCAGGTAGAG GAGGAGGTTG ATGCCATGCT GGCTGTCAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028852.2
CDS	343..1608
Translation	MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

Target ORF information:

RefSeq Version	NM_001033680.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus synaptotagmin 1 (Syt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033680.2

ORF Insert Sequence:

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGC CCTTAAGGAT 
GACGATGCTG AAACCGGACT GACTGATGGA GAAGAAAAGG AAGAGCCCAA GGAAGAGGAG 
AAACTGGGAA AGCTCCAATA TTCACTGGAC TATGACTTCC AGAATAACCA GCTGTTGGTG 
GGAATCATCC AGGCTGCTGA ACTGCCCGCC CTGGACATGG GGGGTACATC CGATCCATAC 
GTCAAAGTCT TCCTGCTGCC TGACAAAAAG AAGAAATTTG AGACTAAAGT CCACCGGAAA 
ACCCTCAATC CAGTCTTCAA TGAACAATTT ACTTTCAAGG TGCCCTACTC GGAATTAGGT 
GGCAAAACCC TGGTGATGGC TGTGTATGAC TTTGATCGCT TCTCCAAGCA TGACATCATC 
GGAGAGTTCA AAGTTCCTAT GAACACCGTG GATTTTGGCC ATGTGACCGA GGAGTGGCGT 
GATCTCCAGA GCGCTGAGAA AGAAGAGCAA GAGAAACTGG GTGACATCTG CTTCTCCCTC 
CGCTACGTCC CTACTGCCGG CAAACTGACT GTTGTCATTC TGGAAGCCAA GAACCTGAAG 
AAGATGGATG TGGGTGGCTT ATCTGATCCC TACGTGAAGA TTCACCTGAT GCAGAACGGT 
AAGAGGCTGA AGAAGAAAAA GACGACGATT AAGAAGAACA CACTCAACCC CTACTACAAC 
GAGTCCTTCA GCTTTGAAGT TCCGTTCGAG CAAATCCAGA AAGTGCAAGT GGTGGTAACT 
GTTTTGGACT ATGACAAGAT TGGCAAGAAC GACGCCATCG GCAAAGTCTT CGTTGGTTAC 
AACAGCACTG GGGCGGAGCT GCGACACTGG TCAGACATGC TGGCCAACCC CCGGCGACCC 
ATCGCACAGT GGCACACTCT GCAGGTAGAG GAGGAGGTTG ATGCCATGCT GGCTGTCAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syt1 ( XM_017594679.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450168.1 Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13122
Clone ID Related Accession (Same CDS sequence)	XM_017594679.1 , NM_001033680.2
Accession Version	XM_017594679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synaptotagmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGC CCTTAAGGAT 
GACGATGCTG AAACCGGACT GACTGATGGA GAAGAAAAGG AAGAGCCCAA GGAAGAGGAG 
AAACTGGGAA AGCTCCAATA TTCACTGGAC TATGACTTCC AGAATAACCA GCTGTTGGTG 
GGAATCATCC AGGCTGCTGA ACTGCCCGCC CTGGACATGG GGGGTACATC CGATCCATAC 
GTCAAAGTCT TCCTGCTGCC TGACAAAAAG AAGAAATTTG AGACTAAAGT CCACCGGAAA 
ACCCTCAATC CAGTCTTCAA TGAACAATTT ACTTTCAAGG TGCCCTACTC GGAATTAGGT 
GGCAAAACCC TGGTGATGGC TGTGTATGAC TTTGATCGCT TCTCCAAGCA TGACATCATC 
GGAGAGTTCA AAGTTCCTAT GAACACCGTG GATTTTGGCC ATGTGACCGA GGAGTGGCGT 
GATCTCCAGA GCGCTGAGAA AGAAGAGCAA GAGAAACTGG GTGACATCTG CTTCTCCCTC 
CGCTACGTCC CTACTGCCGG CAAACTGACT GTTGTCATTC TGGAAGCCAA GAACCTGAAG 
AAGATGGATG TGGGTGGCTT ATCTGATCCC TACGTGAAGA TTCACCTGAT GCAGAACGGT 
AAGAGGCTGA AGAAGAAAAA GACGACGATT AAGAAGAACA CACTCAACCC CTACTACAAC 
GAGTCCTTCA GCTTTGAAGT TCCGTTCGAG CAAATCCAGA AAGTGCAAGT GGTGGTAACT 
GTTTTGGACT ATGACAAGAT TGGCAAGAAC GACGCCATCG GCAAAGTCTT CGTTGGTTAC 
AACAGCACTG GGGCGGAGCT GCGACACTGG TCAGACATGC TGGCCAACCC CCGGCGACCC 
ATCGCACAGT GGCACACTCT GCAGGTAGAG GAGGAGGTTG ATGCCATGCT GGCTGTCAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450168.1
CDS	440..1705
Translation	MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

Target ORF information:

RefSeq Version	XM_017594679.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594679.1

ORF Insert Sequence:

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGC CCTTAAGGAT 
GACGATGCTG AAACCGGACT GACTGATGGA GAAGAAAAGG AAGAGCCCAA GGAAGAGGAG 
AAACTGGGAA AGCTCCAATA TTCACTGGAC TATGACTTCC AGAATAACCA GCTGTTGGTG 
GGAATCATCC AGGCTGCTGA ACTGCCCGCC CTGGACATGG GGGGTACATC CGATCCATAC 
GTCAAAGTCT TCCTGCTGCC TGACAAAAAG AAGAAATTTG AGACTAAAGT CCACCGGAAA 
ACCCTCAATC CAGTCTTCAA TGAACAATTT ACTTTCAAGG TGCCCTACTC GGAATTAGGT 
GGCAAAACCC TGGTGATGGC TGTGTATGAC TTTGATCGCT TCTCCAAGCA TGACATCATC 
GGAGAGTTCA AAGTTCCTAT GAACACCGTG GATTTTGGCC ATGTGACCGA GGAGTGGCGT 
GATCTCCAGA GCGCTGAGAA AGAAGAGCAA GAGAAACTGG GTGACATCTG CTTCTCCCTC 
CGCTACGTCC CTACTGCCGG CAAACTGACT GTTGTCATTC TGGAAGCCAA GAACCTGAAG 
AAGATGGATG TGGGTGGCTT ATCTGATCCC TACGTGAAGA TTCACCTGAT GCAGAACGGT 
AAGAGGCTGA AGAAGAAAAA GACGACGATT AAGAAGAACA CACTCAACCC CTACTACAAC 
GAGTCCTTCA GCTTTGAAGT TCCGTTCGAG CAAATCCAGA AAGTGCAAGT GGTGGTAACT 
GTTTTGGACT ATGACAAGAT TGGCAAGAAC GACGCCATCG GCAAAGTCTT CGTTGGTTAC 
AACAGCACTG GGGCGGAGCT GCGACACTGG TCAGACATGC TGGCCAACCC CCGGCGACCC 
ATCGCACAGT GGCACACTCT GCAGGTAGAG GAGGAGGTTG ATGCCATGCT GGCTGTCAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syt1 ( XM_008765353.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763575.2 Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61909
Clone ID Related Accession (Same CDS sequence)	XM_008765353.2
Accession Version	XM_008765353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synaptotagmin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGA TGACGATGCT 
GAAACCGGAC TGACTGATGG AGAAGAAAAG GAAGAGCCCA AGGAAGAGGA GAAACTGGGA 
AAGCTCCAAT ATTCACTGGA CTATGACTTC CAGAATAACC AGCTGTTGGT GGGAATCATC 
CAGGCTGCTG AACTGCCCGC CCTGGACATG GGGGGTACAT CCGATCCATA CGTCAAAGTC 
TTCCTGCTGC CTGACAAAAA GAAGAAATTT GAGACTAAAG TCCACCGGAA AACCCTCAAT 
CCAGTCTTCA ATGAACAATT TACTTTCAAG GTGCCCTACT CGGAATTAGG TGGCAAAACC 
CTGGTGATGG CTGTGTATGA CTTTGATCGC TTCTCCAAGC ATGACATCAT CGGAGAGTTC 
AAAGTTCCTA TGAACACCGT GGATTTTGGC CATGTGACCG AGGAGTGGCG TGATCTCCAG 
AGCGCTGAGA AAGAAGAGCA AGAGAAACTG GGTGACATCT GCTTCTCCCT CCGCTACGTC 
CCTACTGCCG GCAAACTGAC TGTTGTCATT CTGGAAGCCA AGAACCTGAA GAAGATGGAT 
GTGGGTGGCT TATCTGATCC CTACGTGAAG ATTCACCTGA TGCAGAACGG TAAGAGGCTG 
AAGAAGAAAA AGACGACGAT TAAGAAGAAC ACACTCAACC CCTACTACAA CGAGTCCTTC 
AGCTTTGAAG TTCCGTTCGA GCAAATCCAG AAAGTGCAAG TGGTGGTAAC TGTTTTGGAC 
TATGACAAGA TTGGCAAGAA CGACGCCATC GGCAAAGTCT TCGTTGGTTA CAACAGCACT 
GGGGCGGAGC TGCGACACTG GTCAGACATG CTGGCCAACC CCCGGCGACC CATCGCACAG 
TGGCACACTC TGCAGGTAGA GGAGGAGGTT GATGCCATGC TGGCTGTCAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763575.2
CDS	541..1797
Translation	MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

Target ORF information:

RefSeq Version	XM_008765353.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus synaptotagmin 1 (Syt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765353.2

ORF Insert Sequence:

ATGGTGAGTG CCAGTCATCC TGAGGCCCTG GCCGCCCCTG TCACCACTGT TGCGACCCTT 
GTCCCACACA ATGCCACTGA GCCAGCCAGT CCTGGGGAAG GGAAGGAAGA TGCCTTTTCC 
AAGCTGAAGC AGAAGTTTAT GAATGAGCTG CATAAAATTC CATTGCCACC GTGGGCCTTA 
ATAGCCATAG CCATAGTTGC GGTCCTTTTA GTCGTAACCT GCTGCTTTTG TGTCTGTAAG 
AAATGTTTGT TCAAAAAGAA AAACAAGAAG AAGGGGAAGG AAAAGGGAGG AAAGAACGCC 
ATTAACATGA AAGACGTGAA AGACTTAGGG AAGACCATGA AGGATCAGGA TGACGATGCT 
GAAACCGGAC TGACTGATGG AGAAGAAAAG GAAGAGCCCA AGGAAGAGGA GAAACTGGGA 
AAGCTCCAAT ATTCACTGGA CTATGACTTC CAGAATAACC AGCTGTTGGT GGGAATCATC 
CAGGCTGCTG AACTGCCCGC CCTGGACATG GGGGGTACAT CCGATCCATA CGTCAAAGTC 
TTCCTGCTGC CTGACAAAAA GAAGAAATTT GAGACTAAAG TCCACCGGAA AACCCTCAAT 
CCAGTCTTCA ATGAACAATT TACTTTCAAG GTGCCCTACT CGGAATTAGG TGGCAAAACC 
CTGGTGATGG CTGTGTATGA CTTTGATCGC TTCTCCAAGC ATGACATCAT CGGAGAGTTC 
AAAGTTCCTA TGAACACCGT GGATTTTGGC CATGTGACCG AGGAGTGGCG TGATCTCCAG 
AGCGCTGAGA AAGAAGAGCA AGAGAAACTG GGTGACATCT GCTTCTCCCT CCGCTACGTC 
CCTACTGCCG GCAAACTGAC TGTTGTCATT CTGGAAGCCA AGAACCTGAA GAAGATGGAT 
GTGGGTGGCT TATCTGATCC CTACGTGAAG ATTCACCTGA TGCAGAACGG TAAGAGGCTG 
AAGAAGAAAA AGACGACGAT TAAGAAGAAC ACACTCAACC CCTACTACAA CGAGTCCTTC 
AGCTTTGAAG TTCCGTTCGA GCAAATCCAG AAAGTGCAAG TGGTGGTAAC TGTTTTGGAC 
TATGACAAGA TTGGCAAGAA CGACGCCATC GGCAAAGTCT TCGTTGGTTA CAACAGCACT 
GGGGCGGAGC TGCGACACTG GTCAGACATG CTGGCCAACC CCCGGCGACC CATCGCACAG 
TGGCACACTC TGCAGGTAGA GGAGGAGGTT GATGCCATGC TGGCTGTCAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The calcium sensor synaptotagmin 1 is expressed and regulated in hippocampal postsynaptic spines.
Hippocampus27(11)1168-1177(2017 Nov)
Hussain S,Egbenya DL,Lai YC,Dosa ZJ,S?rensen JB,Anderson AE,Davanger S

Membrane protein Nav1.7 contributes to the persistent post-surgical pain regulated by p-p65 in dorsal root ganglion (DRG) of SMIR rats model.
BMC anesthesiology17(1)150(2017 Nov)
Li Z,Li Y,Cao J,Han X,Cai W,Zang W,Xu J,Zhang W

G?? directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes.
The Journal of biological chemistry292(29)12165-12177(2017 07)
Zurawski Z,Page B,Chicka MC,Brindley RL,Wells CA,Preininger AM,Hyde K,Gilbert JA,Cruz-Rodriguez O,Currie KPM,Chapman ER,Alford S,Hamm HE

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Syt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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