Ptpn11 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptpn11
Entrez Gene ID	25622
Full Name	protein tyrosine phosphatase, non-receptor type 11
Synonyms	Shp2
General protein information	Preferred Names protein tyrosine phosphatase, non-receptor type 11 Names tyrosine-protein phosphatase non-receptor type 11 PTP-1D SH-PTP2 protein tyrosine phosphatase, non-receptor type 11 SHP-2 encodes catalytic domain protein tyrosine phosphatase SH-PTP2 protein-tyrosine phosphatase 1D protein-tyrosine phosphatase SYP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	catalyzes the tyrosine dephosphorylation of para-nitrophenylphosphate, nicotinic acetylcholine receptor, myelin basic protein, and other proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013088.2	NP_037220.2	tyrosine-protein phosphatase non-receptor type 11 isoform 2
NM_001177593.1	NP_001171064.1	tyrosine-protein phosphatase non-receptor type 11 isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PTPN11	NP_990299.1
Danio rerio (zebrafish)	ptpn11a	NP_956140.1
	PTPN11	XP_001110117.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002438	XP_563746.4
Bos taurus (cattle)	PTPN11	XP_002694636.2
Rattus norvegicus (Norway rat)	Ptpn11	NP_001171064.1
Drosophila melanogaster (fruit fly)	csw	NP_001259174.1
Pan troglodytes (chimpanzee)	PTPN11	XP_522535.3
Canis lupus familiaris (dog)	PTPN11	XP_005636307.1
Homo sapiens (human)	PTPN11	NP_002825.3
Mus musculus (house mouse)	Ptpn11	NP_035332.1

Gene Ontology
Bibliography

Related articles in PubMed

Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.
Ganesan R, Rasool M
Molecular immunology91134-144(2017 11)

Src homology 2 domain-containing phosphotyrosine phosphatase 2 (Shp2) controls surface GluA1 protein in synaptic homeostasis.
Zhang B, Lu W
The Journal of biological chemistry292(37)15481-15488(2017 09)

Noonan syndrome-associated SHP2 mutation differentially modulates the expression of postsynaptic receptors according to developmental maturation.
Oh JY, Rhee S, Silva AJ, Lee YS, Kim HK
Neuroscience letters64941-47(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate that IL-17 regulates SHP-2 expression and IL-17RA/STAT-3 dependent production of Cyr61, IL-23, GM-CSF and RANKL in AA-FLS and may reveal a new insight into the pathogenesis of RA.
Title: Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.

Data suggest that phosphorylation of Shp2/Ptpn11 at Tyr542 and its translocation to postsynaptic compartment are integral processes in synaptic scaling/homeostasis; Shp2 phosphatase activity is critical to regulation of Ser(P)845 GluA1 and surface expression of GluA1 during synaptic scaling. (Shp2/Ptpn11 = protein tyrosine phosphatase non-receptor type 11; GluA1 = glutamate receptor ionotropic Ampa1 [alpha 1])
Title: Src homology 2 domain-containing phosphotyrosine phosphatase 2 (Shp2) controls surface GluA1 protein in synaptic homeostasis.

results have revealed a regulatory mechanism of Shp2 underlying LTP and memory, broadening our understanding of Shp2 in cognitive function.
Title: Increased Activity of Src Homology 2 Domain Containing Phosphotyrosine Phosphatase 2 (Shp2) Regulates Activity-dependent AMPA Receptor Trafficking.

results revealed a new role for CryAB, as a modulator of Shp2 phosphatase activity during a functionally relevant stimulus in cardiomyocytes.
Title: ?B-Crystallin interacts and attenuates the tyrosine phosphatase activity of Shp2 in cardiomyocytes under mechanical stress.

PKA phosphorylation of Shp2 protein inhibits its phosphatase activity and modulates ligand specificity
Title: Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its Phosphatase Activity and Modulates Ligand Specificity.

The following Ptpn11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptpn11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12946	NM_001177593.1 Latest version!	Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa12958	NM_013088.2 Latest version!	Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptpn11 ( NM_001177593.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001171064.1 Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12946
Clone ID Related Accession (Same CDS sequence)	NM_001177593.1
Accession Version	NM_001177593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type 11 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07036375.1 and AABR07036376.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U05963.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACATCCC GGAGATGGTT TCACCCCAAT ATCACTGGTG TGGAGGCAGA GAATCTCCTG 
CTGACCCGAG GAGTCGATGG CAGTTTCTTA GCGAGGCCCA GCAAGAGTAA CCCTGGAGAC 
TTCACTCTGT CTGTTAGAAG AAATGGAGCC GTTACCCACA TCAAGATTCA GAACACTGGG 
GACTACTATG ACCTCTATGG CGGGGAAAAG TTTGCCACCT TGGCTGAACT GGTCCAGTAT 
TACATGGAGC ATCACGGGCA GCTGAAAGAG AAGAATGGAG ATGTTATTGA GCTCAAGTAC 
CCACTGAACT GTGCAGACCC TACCTCTGAA AGGTGGTTCC ACGGTCACTT GTCTGGAAAA 
GAAGCAGAGA AGCTGCTGAC AGAGAAGGGG AAGCATGGCA GTTTCCTCGT CCGGGAGAGC 
CAGAGCCACC CTGGGGACTT CGTCCTCTCC GTCCGCACTG GTGATGACAA AGGGGAGAGC 
AATGACAGCA AGTCCAAAGT GACCCATGTC ATGATCCGCT GTCAGGAGCT GAAATATGAT 
GTTGGTGGAG GAGAGCGCTT TGACTCTTTG ACAGACCTGG TGGAGCATTA CAAGAAGAAC 
CCCATGGTGG AGACACTGGG CACAGTCCTG CAGCTCAAAC AGCCCCTCAA CACAACTCGT 
ATTAATGCCG CTGAAATCGA AAGCCGGGTT CGGGAGTTAA GCAAGCTAGC CGAGACCACA 
GATAAAGTCA AACAGGGCTT TTGGGAAGAA TTTGAGACTC TACAGCAACA GGAATGCAAA 
CTTCTCTACA GCCGAAAAGA AGGACAGAGA CAAGAAAATA AAAACAAAAA TAGATACAAA 
AACATCCTGC CCTTTGATCA TACCAGGGTT GTCCTGCACG ATGGGGATCC CAACGAGCCA 
GTTTCTGATT ACATCAATGC CAACATCATC ATGCCTGAAT TTGAAACCAA GTGCAACAAT 
TCAAAACCCA AAAAGAGTTA CATTGCCACT CAAGGCTGCC TGCAGAACAC GGTGAATGAC 
TTCTGGCGGA TGGTGTTCCA GGAGAACTCT CGAGTCATTG TCATGACCAC AAAGGAAGTG 
GAGAGAGGGA AGAGCAAGTG TGTCAAGTAC TGGCCTGATG AGTGTGCACT CAAAGAGTAT 
GGCGTCATGC GTGTGAGGAA CGTCAGAGAA AGTGCTGCGC ATGACTACAC CTTACGAGAA 
CTCAAACTCT CTAAGGTCGG ACAAGCTCTA CTCCAGGGAA ACACAGAGAG AACCGTCTGG 
CAGTACCACT TTCGGACCTG GCCAGACCAC GGTGTGCCTA GTGACCCTGG AGGTGTGCTG 
GACTTCCTGG AGGAGGTCCA CCACAAGCAG GAGAGCATCG TGGATGCAGG CCCTGTCGTG 
GTTCACTGCA GTGCTGGGAT TGGCCGGACA GGAACGTTCA TTGTGATTGA TATCCTTATT 
GACATCATCC GAGAGAAAGG TGTGGACTGT GACATCGACG TTCCTAAAAC CATTCAGATG 
GTACGGTCCC AGAGGTCAGG GATGGTCCAG ACAGAAGCAC AGTACCGGTT CATCTACATG 
GCCGTCCAGC ACTACATAGA GACGCTGCAA CGCAGGATCG AGGAGGAGCA GAAAAGCAAA 
AGGAAAGGAC ATGAATATAC CAATATTAAG TATTCCCTGG TGGACCAGAC AAGTGGCGAT 
CAGAGTCCCC TGCCACCTTG CACCCCAACG CCACCCTGTG CGGAAATGCG GGAGGACAGC 
GCTCGAGTCT ATGAGAACGT GGGCCTCATG CAGCAGCAGA GGAGTTTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171064.1
CDS	177..1970
Translation	MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDSKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDECALKEYGVMRVRNVRESAAHDYTLRELKLSKVGQALLQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR

Target ORF information:

RefSeq Version	NM_001177593.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177593.1

ORF Insert Sequence:

ATGACATCCC GGAGATGGTT TCACCCCAAT ATCACTGGTG TGGAGGCAGA GAATCTCCTG 
CTGACCCGAG GAGTCGATGG CAGTTTCTTA GCGAGGCCCA GCAAGAGTAA CCCTGGAGAC 
TTCACTCTGT CTGTTAGAAG AAATGGAGCC GTTACCCACA TCAAGATTCA GAACACTGGG 
GACTACTATG ACCTCTATGG CGGGGAAAAG TTTGCCACCT TGGCTGAACT GGTCCAGTAT 
TACATGGAGC ATCACGGGCA GCTGAAAGAG AAGAATGGAG ATGTTATTGA GCTCAAGTAC 
CCACTGAACT GTGCAGACCC TACCTCTGAA AGGTGGTTCC ACGGTCACTT GTCTGGAAAA 
GAAGCAGAGA AGCTGCTGAC AGAGAAGGGG AAGCATGGCA GTTTCCTCGT CCGGGAGAGC 
CAGAGCCACC CTGGGGACTT CGTCCTCTCC GTCCGCACTG GTGATGACAA AGGGGAGAGC 
AATGACAGCA AGTCCAAAGT GACCCATGTC ATGATCCGCT GTCAGGAGCT GAAATATGAT 
GTTGGTGGAG GAGAGCGCTT TGACTCTTTG ACAGACCTGG TGGAGCATTA CAAGAAGAAC 
CCCATGGTGG AGACACTGGG CACAGTCCTG CAGCTCAAAC AGCCCCTCAA CACAACTCGT 
ATTAATGCCG CTGAAATCGA AAGCCGGGTT CGGGAGTTAA GCAAGCTAGC CGAGACCACA 
GATAAAGTCA AACAGGGCTT TTGGGAAGAA TTTGAGACTC TACAGCAACA GGAATGCAAA 
CTTCTCTACA GCCGAAAAGA AGGACAGAGA CAAGAAAATA AAAACAAAAA TAGATACAAA 
AACATCCTGC CCTTTGATCA TACCAGGGTT GTCCTGCACG ATGGGGATCC CAACGAGCCA 
GTTTCTGATT ACATCAATGC CAACATCATC ATGCCTGAAT TTGAAACCAA GTGCAACAAT 
TCAAAACCCA AAAAGAGTTA CATTGCCACT CAAGGCTGCC TGCAGAACAC GGTGAATGAC 
TTCTGGCGGA TGGTGTTCCA GGAGAACTCT CGAGTCATTG TCATGACCAC AAAGGAAGTG 
GAGAGAGGGA AGAGCAAGTG TGTCAAGTAC TGGCCTGATG AGTGTGCACT CAAAGAGTAT 
GGCGTCATGC GTGTGAGGAA CGTCAGAGAA AGTGCTGCGC ATGACTACAC CTTACGAGAA 
CTCAAACTCT CTAAGGTCGG ACAAGCTCTA CTCCAGGGAA ACACAGAGAG AACCGTCTGG 
CAGTACCACT TTCGGACCTG GCCAGACCAC GGTGTGCCTA GTGACCCTGG AGGTGTGCTG 
GACTTCCTGG AGGAGGTCCA CCACAAGCAG GAGAGCATCG TGGATGCAGG CCCTGTCGTG 
GTTCACTGCA GTGCTGGGAT TGGCCGGACA GGAACGTTCA TTGTGATTGA TATCCTTATT 
GACATCATCC GAGAGAAAGG TGTGGACTGT GACATCGACG TTCCTAAAAC CATTCAGATG 
GTACGGTCCC AGAGGTCAGG GATGGTCCAG ACAGAAGCAC AGTACCGGTT CATCTACATG 
GCCGTCCAGC ACTACATAGA GACGCTGCAA CGCAGGATCG AGGAGGAGCA GAAAAGCAAA 
AGGAAAGGAC ATGAATATAC CAATATTAAG TATTCCCTGG TGGACCAGAC AAGTGGCGAT 
CAGAGTCCCC TGCCACCTTG CACCCCAACG CCACCCTGTG CGGAAATGCG GGAGGACAGC 
GCTCGAGTCT ATGAGAACGT GGGCCTCATG CAGCAGCAGA GGAGTTTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptpn11 ( NM_013088.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037220.2 Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12958
Clone ID Related Accession (Same CDS sequence)	NM_013088.2
Accession Version	NM_013088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type 11 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07036375.1 and AABR07036376.1. On Apr 20, 2010 this sequence version replaced NM_013088.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal 4-aa segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACATCCC GGAGATGGTT TCACCCCAAT ATCACTGGTG TGGAGGCAGA GAATCTCCTG 
CTGACCCGAG GAGTCGATGG CAGTTTCTTA GCGAGGCCCA GCAAGAGTAA CCCTGGAGAC 
TTCACTCTGT CTGTTAGAAG AAATGGAGCC GTTACCCACA TCAAGATTCA GAACACTGGG 
GACTACTATG ACCTCTATGG CGGGGAAAAG TTTGCCACCT TGGCTGAACT GGTCCAGTAT 
TACATGGAGC ATCACGGGCA GCTGAAAGAG AAGAATGGAG ATGTTATTGA GCTCAAGTAC 
CCACTGAACT GTGCAGACCC TACCTCTGAA AGGTGGTTCC ACGGTCACTT GTCTGGAAAA 
GAAGCAGAGA AGCTGCTGAC AGAGAAGGGG AAGCATGGCA GTTTCCTCGT CCGGGAGAGC 
CAGAGCCACC CTGGGGACTT CGTCCTCTCC GTCCGCACTG GTGATGACAA AGGGGAGAGC 
AATGACAGCA AGTCCAAAGT GACCCATGTC ATGATCCGCT GTCAGGAGCT GAAATATGAT 
GTTGGTGGAG GAGAGCGCTT TGACTCTTTG ACAGACCTGG TGGAGCATTA CAAGAAGAAC 
CCCATGGTGG AGACACTGGG CACAGTCCTG CAGCTCAAAC AGCCCCTCAA CACAACTCGT 
ATTAATGCCG CTGAAATCGA AAGCCGGGTT CGGGAGTTAA GCAAGCTAGC CGAGACCACA 
GATAAAGTCA AACAGGGCTT TTGGGAAGAA TTTGAGACTC TACAGCAACA GGAATGCAAA 
CTTCTCTACA GCCGAAAAGA AGGACAGAGA CAAGAAAATA AAAACAAAAA TAGATACAAA 
AACATCCTGC CCTTTGATCA TACCAGGGTT GTCCTGCACG ATGGGGATCC CAACGAGCCA 
GTTTCTGATT ACATCAATGC CAACATCATC ATGCCTGAAT TTGAAACCAA GTGCAACAAT 
TCAAAACCCA AAAAGAGTTA CATTGCCACT CAAGGCTGCC TGCAGAACAC GGTGAATGAC 
TTCTGGCGGA TGGTGTTCCA GGAGAACTCT CGAGTCATTG TCATGACCAC AAAGGAAGTG 
GAGAGAGGGA AGAGCAAGTG TGTCAAGTAC TGGCCTGATG AGTGTGCACT CAAAGAGTAT 
GGCGTCATGC GTGTGAGGAA CGTCAGAGAA AGTGCTGCGC ATGACTACAC CTTACGAGAA 
CTCAAACTCT CTAAGGTCGG ACAAGGAAAC ACAGAGAGAA CCGTCTGGCA GTACCACTTT 
CGGACCTGGC CAGACCACGG TGTGCCTAGT GACCCTGGAG GTGTGCTGGA CTTCCTGGAG 
GAGGTCCACC ACAAGCAGGA GAGCATCGTG GATGCAGGCC CTGTCGTGGT TCACTGCAGT 
GCTGGGATTG GCCGGACAGG AACGTTCATT GTGATTGATA TCCTTATTGA CATCATCCGA 
GAGAAAGGTG TGGACTGTGA CATCGACGTT CCTAAAACCA TTCAGATGGT ACGGTCCCAG 
AGGTCAGGGA TGGTCCAGAC AGAAGCACAG TACCGGTTCA TCTACATGGC CGTCCAGCAC 
TACATAGAGA CGCTGCAACG CAGGATCGAG GAGGAGCAGA AAAGCAAAAG GAAAGGACAT 
GAATATACCA ATATTAAGTA TTCCCTGGTG GACCAGACAA GTGGCGATCA GAGTCCCCTG 
CCACCTTGCA CCCCAACGCC ACCCTGTGCG GAAATGCGGG AGGACAGCGC TCGAGTCTAT 
GAGAACGTGG GCCTCATGCA GCAGCAGAGG AGTTTCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037220.2
CDS	177..1958
Translation	MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDSKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDECALKEYGVMRVRNVRESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR

Target ORF information:

RefSeq Version	NM_013088.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine phosphatase, non-receptor type 11 (Ptpn11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013088.2

ORF Insert Sequence:

ATGACATCCC GGAGATGGTT TCACCCCAAT ATCACTGGTG TGGAGGCAGA GAATCTCCTG 
CTGACCCGAG GAGTCGATGG CAGTTTCTTA GCGAGGCCCA GCAAGAGTAA CCCTGGAGAC 
TTCACTCTGT CTGTTAGAAG AAATGGAGCC GTTACCCACA TCAAGATTCA GAACACTGGG 
GACTACTATG ACCTCTATGG CGGGGAAAAG TTTGCCACCT TGGCTGAACT GGTCCAGTAT 
TACATGGAGC ATCACGGGCA GCTGAAAGAG AAGAATGGAG ATGTTATTGA GCTCAAGTAC 
CCACTGAACT GTGCAGACCC TACCTCTGAA AGGTGGTTCC ACGGTCACTT GTCTGGAAAA 
GAAGCAGAGA AGCTGCTGAC AGAGAAGGGG AAGCATGGCA GTTTCCTCGT CCGGGAGAGC 
CAGAGCCACC CTGGGGACTT CGTCCTCTCC GTCCGCACTG GTGATGACAA AGGGGAGAGC 
AATGACAGCA AGTCCAAAGT GACCCATGTC ATGATCCGCT GTCAGGAGCT GAAATATGAT 
GTTGGTGGAG GAGAGCGCTT TGACTCTTTG ACAGACCTGG TGGAGCATTA CAAGAAGAAC 
CCCATGGTGG AGACACTGGG CACAGTCCTG CAGCTCAAAC AGCCCCTCAA CACAACTCGT 
ATTAATGCCG CTGAAATCGA AAGCCGGGTT CGGGAGTTAA GCAAGCTAGC CGAGACCACA 
GATAAAGTCA AACAGGGCTT TTGGGAAGAA TTTGAGACTC TACAGCAACA GGAATGCAAA 
CTTCTCTACA GCCGAAAAGA AGGACAGAGA CAAGAAAATA AAAACAAAAA TAGATACAAA 
AACATCCTGC CCTTTGATCA TACCAGGGTT GTCCTGCACG ATGGGGATCC CAACGAGCCA 
GTTTCTGATT ACATCAATGC CAACATCATC ATGCCTGAAT TTGAAACCAA GTGCAACAAT 
TCAAAACCCA AAAAGAGTTA CATTGCCACT CAAGGCTGCC TGCAGAACAC GGTGAATGAC 
TTCTGGCGGA TGGTGTTCCA GGAGAACTCT CGAGTCATTG TCATGACCAC AAAGGAAGTG 
GAGAGAGGGA AGAGCAAGTG TGTCAAGTAC TGGCCTGATG AGTGTGCACT CAAAGAGTAT 
GGCGTCATGC GTGTGAGGAA CGTCAGAGAA AGTGCTGCGC ATGACTACAC CTTACGAGAA 
CTCAAACTCT CTAAGGTCGG ACAAGGAAAC ACAGAGAGAA CCGTCTGGCA GTACCACTTT 
CGGACCTGGC CAGACCACGG TGTGCCTAGT GACCCTGGAG GTGTGCTGGA CTTCCTGGAG 
GAGGTCCACC ACAAGCAGGA GAGCATCGTG GATGCAGGCC CTGTCGTGGT TCACTGCAGT 
GCTGGGATTG GCCGGACAGG AACGTTCATT GTGATTGATA TCCTTATTGA CATCATCCGA 
GAGAAAGGTG TGGACTGTGA CATCGACGTT CCTAAAACCA TTCAGATGGT ACGGTCCCAG 
AGGTCAGGGA TGGTCCAGAC AGAAGCACAG TACCGGTTCA TCTACATGGC CGTCCAGCAC 
TACATAGAGA CGCTGCAACG CAGGATCGAG GAGGAGCAGA AAAGCAAAAG GAAAGGACAT 
GAATATACCA ATATTAAGTA TTCCCTGGTG GACCAGACAA GTGGCGATCA GAGTCCCCTG 
CCACCTTGCA CCCCAACGCC ACCCTGTGCG GAAATGCGGG AGGACAGCGC TCGAGTCTAT 
GAGAACGTGG GCCTCATGCA GCAGCAGAGG AGTTTCAGAT GA