F3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F3
Entrez Gene ID	25584
Full Name	coagulation factor III, tissue factor
General protein information	Preferred Names coagulation factor III, tissue factor Names tissue factor TF coagulation factor 3 coagulation factor III (thromboplastin, tissue factor)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q41
Summary	an initiation factor for blood coagulation; plays a critical role in hepatic ischemic reperfusion injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013057.2	NP_037189.2	tissue factor precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	f3	XP_002933837.1
Pan troglodytes (chimpanzee)	F3	XP_001156450.1
Homo sapiens (human)	F3	NP_001984.1
Canis lupus familiaris (dog)	F3	NP_001019811.1
Mus musculus (house mouse)	F3	NP_034301.3
Rattus norvegicus (Norway rat)	F3	NP_037189.2
Bos taurus (cattle)	F3	NP_776303.1
Gallus gallus (chicken)	F3	XP_426640.2
Danio rerio (zebrafish)	f3b	NP_001017728.1
Danio rerio (zebrafish)	f3a	NP_001232896.1

Gene Ontology
Bibliography

Related articles in PubMed

A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis.
Jin W, Yin Y, Zhang B, Mei H, Wang H, Guo T, Shi W, Hu Y
Journal of biomaterials applications32(3)342-348(2017 Sep)

Cytoplasmic domain of tissue factor promotes liver fibrosis in mice.
Knight V, Lourensz D, Tchongue J, Correia J, Tipping P, Sievert W
World journal of gastroenterology23(31)5692-5699(2017 Aug)

Investigation for role of tissue factor and blood coagulation system in severe acute pancreatitis and associated liver injury.
Ou ZB, Miao CM, Ye MX, Xing DP, He K, Li PZ, Zhu RT, Gong JP
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85380-388(2017 Jan)

Expression of tissue factor and forkhead box transcription factor O-1 in a rat model for chronic thromboembolic pulmonary hypertension.
Deng C, Wu D, Yang M, Chen Y, Wang C, Zhong Z, Lian N, Chen H, Wu S
Journal of thrombosis and thrombolysis42(4)520-8(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

rat model of chronic thromboembolic pulmonary hypertension can be successfully established by the injection of autologous blood clots into the pulmonary artery. TF and FoxO1 may play a key role in vascular remodeling during chronic thromboembolic pulmonary hypertension pathogenesis.
Title: Expression of tissue factor and forkhead box transcription factor O-1 in a rat model for chronic thromboembolic pulmonary hypertension.

Toll-like receptor 3 plays a key role of in tissue factor activation through extracellular signal regulated kinase 1/2 pathway in rat hypoxia.
Title: A key role of toll-like receptor 3 in tissue factor activation through extracellular signal regulated kinase 1/2 pathway in a murine hypoxia model.

Treatment with irbesartan plus carvedilol reduced the expression of TF and TFPI mRNA and protein after myocardial infarction in rats.
Title: Combined effects of irbesartan and carvedilol on expression of tissue factor and tissue factor pathway inhibitor in rats after myocardial infarction.

transferable factor(s) mediate the ischemia-reperfusion injury-induced decrease in NHE3 of the kidney.
Title: The reduction of Na/H exchanger-3 protein and transcript expression in acute ischemia-reperfusion injury is mediated by extractable tissue factor(s).

Facilitation of platelet aggregation by exogenous asymmetric dimethylarginine in hypertensive rats is related to upregulation of the level and activity of plasma tissue factor.
Title: Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor.

The following F3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01806	NM_013057.2 Latest version!	Rattus norvegicus coagulation factor III, tissue factor (F3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F3 ( NM_013057.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037189.2 Rattus norvegicus coagulation factor III, tissue factor (F3), mRNA.

Price & Availability↑

CloneID	ORa01806
Clone ID Related Accession (Same CDS sequence)	NM_013057.2
Accession Version	NM_013057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	tissue factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081846.1. On Sep 16, 2004 this sequence version replaced NM_013057.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081846.1, U07619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTATCC CCATGCGCCC GCGCCTCCTA GCGGCCCTCG CGCCCACCTT TCTCGGCTTC 
CTTCTCCTTC AGGTGGCCGC TGGTGCAGGC ACTCCTCCAG GGAAAGCGTT TAATTTAACT 
TGGATATCAA CTGATTTCAA GACAATCTTG GAGTGGCAAC CGAAACCCAC CAACTATACC 
TACACTGTTC AGATAAGCGA TAGATCTAGA AACTGGAAAT ACAAATGCAC TGGAACCACA 
GACACTGAGT GTGACCTCAC CGACGAGATT GTGAAGGATG TGAACTGGAC CTATGAAGCA 
AGGGTCCTAT CTGTCCCATG GAGGAACTCA ACTCATGGAA AGGAAACACT CTTTGGAACT 
CATGGGGAGG AACCGCCATT TACAAATGCC CGGAAGTTTT TACCTTACCG AGACACAAAA 
ATTGGACAGC CAGTAATTCA GAAGTATGAA CAAGACGGTA CGAAACTGAA GGTGACTGTA 
AAAGACTCAT TTACATTAGT CAGAAAGAAT GGTACATTCC TCACTCTGCG GCAAGTTTTT 
GGCAATGACT TGGGTTATAT TCTTACGTAT CGGAAAGACT CAAGCACAGG AAGGAAAACA 
AACACTACAC ATACCAATGA ATTCTTGATT GATGTGGAAA AGGGGGTAAG CTACTGCTTC 
TTCGTACAAG CCGTGATTTT CTCCAGGAAA ACTAACCACA AGAGCCCAGA AAGCATCACC 
AAGTGCACTG AGCAATGGAA GAGTGTCCTG GGAGAAACAC TCATCATTGT GGGAGCAGTG 
GTGTTCCTGG TCACTGTCTT TATCATCCTG CTGACCATAT CTCTGTGCAA GCGCAGAAAG 
AACAGAGCAG GACAGAAAAG GAAGAACACC CCGTCGCGCT TGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037189.2
CDS	97..984
Translation	MAIPMRPRLLAALAPTFLGFLLLQVAAGAGTPPGKAFNLTWISTDFKTILEWQPKPTNYTYTVQISDRSRNWKYKCTGTTDTECDLTDEIVKDVNWTYEARVLSVPWRNSTHGKETLFGTHGEEPPFTNARKFLPYRDTKIGQPVIQKYEQDGTKLKVTVKDSFTLVRKNGTFLTLRQVFGNDLGYILTYRKDSSTGRKTNTTHTNEFLIDVEKGVSYCFFVQAVIFSRKTNHKSPESITKCTEQWKSVLGETLIIVGAVVFLVTVFIILLTISLCKRRKNRAGQKRKNTPSRLA

Target ORF information:

RefSeq Version	NM_013057.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coagulation factor III, tissue factor (F3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013057.2

ORF Insert Sequence:

ATGGCTATCC CCATGCGCCC GCGCCTCCTA GCGGCCCTCG CGCCCACCTT TCTCGGCTTC 
CTTCTCCTTC AGGTGGCCGC TGGTGCAGGC ACTCCTCCAG GGAAAGCGTT TAATTTAACT 
TGGATATCAA CTGATTTCAA GACAATCTTG GAGTGGCAAC CGAAACCCAC CAACTATACC 
TACACTGTTC AGATAAGCGA TAGATCTAGA AACTGGAAAT ACAAATGCAC TGGAACCACA 
GACACTGAGT GTGACCTCAC CGACGAGATT GTGAAGGATG TGAACTGGAC CTATGAAGCA 
AGGGTCCTAT CTGTCCCATG GAGGAACTCA ACTCATGGAA AGGAAACACT CTTTGGAACT 
CATGGGGAGG AACCGCCATT TACAAATGCC CGGAAGTTTT TACCTTACCG AGACACAAAA 
ATTGGACAGC CAGTAATTCA GAAGTATGAA CAAGACGGTA CGAAACTGAA GGTGACTGTA 
AAAGACTCAT TTACATTAGT CAGAAAGAAT GGTACATTCC TCACTCTGCG GCAAGTTTTT 
GGCAATGACT TGGGTTATAT TCTTACGTAT CGGAAAGACT CAAGCACAGG AAGGAAAACA 
AACACTACAC ATACCAATGA ATTCTTGATT GATGTGGAAA AGGGGGTAAG CTACTGCTTC 
TTCGTACAAG CCGTGATTTT CTCCAGGAAA ACTAACCACA AGAGCCCAGA AAGCATCACC 
AAGTGCACTG AGCAATGGAA GAGTGTCCTG GGAGAAACAC TCATCATTGT GGGAGCAGTG 
GTGTTCCTGG TCACTGTCTT TATCATCCTG CTGACCATAT CTCTGTGCAA GCGCAGAAAG 
AACAGAGCAG GACAGAAAAG GAAGAACACC CCGTCGCGCT TGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis.
Journal of biomaterials applications32(3)342-348(2017 Sep)
Jin W,Yin Y,Zhang B,Mei H,Wang H,Guo T,Shi W,Hu Y

Cytoplasmic domain of tissue factor promotes liver fibrosis in mice.
World journal of gastroenterology23(31)5692-5699(2017 Aug)
Knight V,Lourensz D,Tchongue J,Correia J,Tipping P,Sievert W

Investigation for role of tissue factor and blood coagulation system in severe acute pancreatitis and associated liver injury.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85380-388(2017 Jan)
Ou ZB,Miao CM,Ye MX,Xing DP,He K,Li PZ,Zhu RT,Gong JP

Expression of tissue factor and forkhead box transcription factor O-1 in a rat model for chronic thromboembolic pulmonary hypertension.
Journal of thrombosis and thrombolysis42(4)520-8(2016 Nov)
Deng C,Wu D,Yang M,Chen Y,Wang C,Zhong Z,Lian N,Chen H,Wu S