Agrp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agrp
Entrez Gene ID	25582
Full Name	agouti related neuropeptide
General protein information	Preferred Names agouti related neuropeptide Names agouti-related protein agouti related protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	may interact with melanocortin receptors; may play a role in appetite stimulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772474.2	XP_008770696.1	agouti-related protein isoform X1
NM_033650.1	NP_387499.1	agouti-related protein precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AGRP	XP_853932.1
Pan troglodytes (chimpanzee)	AGRP	XP_001164015.1
	AGRP	XP_001091740.1
Bos taurus (cattle)	AGRP	NP_776408.1
Mus musculus (house mouse)	Agrp	NP_001258735.1
Homo sapiens (human)	AGRP	NP_001129.1
Rattus norvegicus (Norway rat)	Agrp	NP_387499.1
Gallus gallus (chicken)	AGRP	NP_001026628.1
Gallus gallus (chicken)	LOC100859622	XP_004951049.1

Gene Ontology
Bibliography

Related articles in PubMed

Failure to upregulate Agrp and Orexin in response to activity based anorexia in weight loss vulnerable rats characterized by passive stress coping and prenatal stress experience.
Boersma GJ, Liang NC, Lee RS, Albertz JD, Kastelein A, Moody LA, Aryal S, Moran TH, Tamashiro KL
Psychoneuroendocrinology67171-81(2016 May)

[Participation of Agouti related peptide in machanisms of wakefulness-sleep cycle regulation].
Romanova IV, Mikhrina AL
Fiziologiia cheloveka39(6)24-30(2013 Nov-Dec)

Glucocorticoids are required for meal-induced changes in the expression of hypothalamic neuropeptides.
Uchoa ET, Silva LE, de Castro M, Antunes-Rodrigues J, Elias LL
Neuropeptides46(3)119-24(2012 Jun)

Short-term regulation of the hypothalamic melanocortinergic system under fasting and defined glucose-refeeding conditions in rats: a laser capture microdissection (LMD)-based study.
Landmann EM, Schellong K, Melchior K, Rodekamp E, Ziska T, Harder T, Plagemann A
Neuroscience letters515(1)87-91(2012 Apr)

Green tea polyphenols reduce body weight in rats by modulating obesity-related genes.
Lu C, Zhu W, Shen CL, Gao W
PloS one7(6)e38332(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study suggests that passive PNS rats have innate impairments in leptin and ghrelin responses to starvation combined with prenatal stress associated impairments in Agrp and orexin expression in response to starvation.
Title: Failure to upregulate Agrp and Orexin in response to activity based anorexia in weight loss vulnerable rats characterized by passive stress coping and prenatal stress experience.

The obtained data demonstrate the role ofAGRP as a modulator of the functional activity of the dopaminergic brain neurons.
Title: [Participation of Agouti related peptide in machanisms of wakefulness-sleep cycle regulation].

AGRP is up- and down-regulated in the hypothalamic arcuate nucleus depending on the general nutritional state.
Title: Short-term regulation of the hypothalamic melanocortinergic system under fasting and defined glucose-refeeding conditions in rats: a laser capture microdissection (LMD)-based study.

Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, Agrp, Ghrl, and Nr3c1, Apoa4, Cntf, Ghr, IL-1beta, Ins1, Lepr,Sort, Adcyap1r1 and Adrb1.
Title: Green tea polyphenols reduce body weight in rats by modulating obesity-related genes.

Mature neurons were obtained from differentiation of hypothalamic progenitor cells including NPY, AGRP, POMC, CART and Orexin-A positive neurons. mRNA expression of NPY, CART and Orexin-A increases after the differentiation of hypothalamic neurospheres
Title: Proliferative hypothalamic neurospheres express NPY, AGRP, POMC, CART and Orexin-A and differentiate to functional neurons.

The following Agrp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agrp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09131	NM_033650.1 Latest version!	Rattus norvegicus agouti related neuropeptide (Agrp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa09131	XM_008772474.2 Latest version!	Rattus norvegicus agouti related neuropeptide (Agrp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agrp ( NM_033650.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_387499.1 Rattus norvegicus agouti related neuropeptide (Agrp), mRNA.

Price & Availability↑

CloneID	ORa09131
Clone ID Related Accession (Same CDS sequence)	XM_008772474.2 , NM_033650.1
Accession Version	NM_033650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	agouti-related protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116220.6. On or before Dec 13, 2009 this sequence version replaced XM_001075738.1, XM_574228.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTGACTG CAATGTTGCT GAGCTGTGTC CTGCTGCTGG CACTGCCTCC CACATTGGGG 
GTCCATATGG GCGTGGCACC ACTGAAGGGC ATCAGAAGGT CTGACCAGGC CCTGTTCCCA 
GAGTTCTCAG GTCTAAGTCT GAAGAAGACA GCAGCAGACC GAGCAGAAGA TGTTCTGCTG 
CAGAAGGCAG AAGCTTTGGC AGAGGTGCTA GATCCACAGA ACCGCGAGTC TCGTTCTCCG 
CGTCGCTGTG TAAGGCTGCA CGAGTCCTGC TTGGGACAAC AGGTACCTTG CTGCGACCTG 
TGCGCCACGT GCTACTGCCG CTTCTTCAAT ACCTTTTGCT ACTGCCGCAA GCTAGGTACT 
GGCACCACGA ACCTCTGCAG CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_387499.1
CDS	208..597
Translation	MLTAMLLSCVLLLALPPTLGVHMGVAPLKGIRRSDQALFPEFSGLSLKKTAADRAEDVLLQKAEALAEVLDPQNRESRSPRRCVRLHESCLGQQVPCCDLCATCYCRFFNTFCYCRKLGTGTTNLCSRP

Target ORF information:

RefSeq Version	NM_033650.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agouti related neuropeptide (Agrp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033650.1

ORF Insert Sequence:

ATGCTGACTG CAATGTTGCT GAGCTGTGTC CTGCTGCTGG CACTGCCTCC CACATTGGGG 
GTCCATATGG GCGTGGCACC ACTGAAGGGC ATCAGAAGGT CTGACCAGGC CCTGTTCCCA 
GAGTTCTCAG GTCTAAGTCT GAAGAAGACA GCAGCAGACC GAGCAGAAGA TGTTCTGCTG 
CAGAAGGCAG AAGCTTTGGC AGAGGTGCTA GATCCACAGA ACCGCGAGTC TCGTTCTCCG 
CGTCGCTGTG TAAGGCTGCA CGAGTCCTGC TTGGGACAAC AGGTACCTTG CTGCGACCTG 
TGCGCCACGT GCTACTGCCG CTTCTTCAAT ACCTTTTGCT ACTGCCGCAA GCTAGGTACT 
GGCACCACGA ACCTCTGCAG CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrp ( XM_008772474.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770696.1 Rattus norvegicus agouti related neuropeptide (Agrp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa09131
Clone ID Related Accession (Same CDS sequence)	XM_008772474.2 , NM_033650.1
Accession Version	XM_008772474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	agouti-related protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACTG CAATGTTGCT GAGCTGTGTC CTGCTGCTGG CACTGCCTCC CACATTGGGG 
GTCCATATGG GCGTGGCACC ACTGAAGGGC ATCAGAAGGT CTGACCAGGC CCTGTTCCCA 
GAGTTCTCAG GTCTAAGTCT GAAGAAGACA GCAGCAGACC GAGCAGAAGA TGTTCTGCTG 
CAGAAGGCAG AAGCTTTGGC AGAGGTGCTA GATCCACAGA ACCGCGAGTC TCGTTCTCCG 
CGTCGCTGTG TAAGGCTGCA CGAGTCCTGC TTGGGACAAC AGGTACCTTG CTGCGACCTG 
TGCGCCACGT GCTACTGCCG CTTCTTCAAT ACCTTTTGCT ACTGCCGCAA GCTAGGTACT 
GGCACCACGA ACCTCTGCAG CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770696.1
CDS	352..741
Translation	MLTAMLLSCVLLLALPPTLGVHMGVAPLKGIRRSDQALFPEFSGLSLKKTAADRAEDVLLQKAEALAEVLDPQNRESRSPRRCVRLHESCLGQQVPCCDLCATCYCRFFNTFCYCRKLGTGTTNLCSRP

Target ORF information:

RefSeq Version	XM_008772474.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agouti related neuropeptide (Agrp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772474.2

ORF Insert Sequence:

ATGCTGACTG CAATGTTGCT GAGCTGTGTC CTGCTGCTGG CACTGCCTCC CACATTGGGG 
GTCCATATGG GCGTGGCACC ACTGAAGGGC ATCAGAAGGT CTGACCAGGC CCTGTTCCCA 
GAGTTCTCAG GTCTAAGTCT GAAGAAGACA GCAGCAGACC GAGCAGAAGA TGTTCTGCTG 
CAGAAGGCAG AAGCTTTGGC AGAGGTGCTA GATCCACAGA ACCGCGAGTC TCGTTCTCCG 
CGTCGCTGTG TAAGGCTGCA CGAGTCCTGC TTGGGACAAC AGGTACCTTG CTGCGACCTG 
TGCGCCACGT GCTACTGCCG CTTCTTCAAT ACCTTTTGCT ACTGCCGCAA GCTAGGTACT 
GGCACCACGA ACCTCTGCAG CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Failure to upregulate Agrp and Orexin in response to activity based anorexia in weight loss vulnerable rats characterized by passive stress coping and prenatal stress experience.
Psychoneuroendocrinology67171-81(2016 May)
Boersma GJ,Liang NC,Lee RS,Albertz JD,Kastelein A,Moody LA,Aryal S,Moran TH,Tamashiro KL

[Participation of Agouti related peptide in machanisms of wakefulness-sleep cycle regulation].
Fiziologiia cheloveka39(6)24-30(2013 Nov-Dec)
Romanova IV,Mikhrina AL

Glucocorticoids are required for meal-induced changes in the expression of hypothalamic neuropeptides.
Neuropeptides46(3)119-24(2012 Jun)
Uchoa ET,Silva LE,de Castro M,Antunes-Rodrigues J,Elias LL

Short-term regulation of the hypothalamic melanocortinergic system under fasting and defined glucose-refeeding conditions in rats: a laser capture microdissection (LMD)-based study.
Neuroscience letters515(1)87-91(2012 Apr)
Landmann EM,Schellong K,Melchior K,Rodekamp E,Ziska T,Harder T,Plagemann A

Green tea polyphenols reduce body weight in rats by modulating obesity-related genes.
PloS one7(6)e38332(2012)
Lu C,Zhu W,Shen CL,Gao W