Rab4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab4a
Entrez Gene ID	25532
Full Name	RAB4A, member RAS oncogene family
Synonyms	Rab4
General protein information	Preferred Names RAB4A, member RAS oncogene family Names ras-related protein Rab-4A Ras-related small GTP binding protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	may play a role in intracellular recruitment of the glucose transporter GLUT4 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772600.2	XP_008770822.1	ras-related protein Rab-4A isoform X1
NM_013019.2	NP_037151.2	ras-related protein Rab-4A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RAB4A	NP_001033774.1
Schizosaccharomyces pombe (fission yeast)	ypt4	NP_594796.1
	RAB4A	XP_001082985.1
Canis lupus familiaris (dog)	RAB4A	XP_005618898.1
Rattus norvegicus (Norway rat)	Rab4a	NP_037151.2
Danio rerio (zebrafish)	rab4a	NP_001119853.1
Magnaporthe oryzae (rice blast fungus)	MGG_01179	XP_003714063.1
Mus musculus (house mouse)	Rab4a	NP_033029.2
Gallus gallus (chicken)	RAB4A	XP_419573.1
Neurospora crassa	NCU00889	XP_964805.2
Homo sapiens (human)	RAB4A	NP_004569.2

Gene Ontology
Bibliography

Related articles in PubMed

Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons.
Jackson TC, Kotermanski SE, Kochanek PM
Neuroscience36193-107(2017 Oct)

Neovascularization in the pulmonary endothelium is regulated by the endosome: Rab4-mediated trafficking and p18-dependent signaling.
Chichger H, Braza J, Duong H, Stark M, Harrington EO
American journal of physiology. Lung cellular and molecular physiology309(7)L700-9(2015 Oct)

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.
Kuijl C, Pilli M, Alahari SK, Janssen H, Khoo PS, Ervin KE, Calero M, Jonnalagadda S, Scheller RH, Neefjes J, Junutula JR
The EMBO journal32(5)713-27(2013 Mar)

Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.
Peng HY, Chen GD, Hsieh MC, Lai CY, Huang YP, Lin TB
Pain153(12)2380-92(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study shows for the first time that neovascularization within the pulmonary vasculature is dependent on the prorecycling endocytic proteins Rab4 and p18.
Title: Neovascularization in the pulmonary endothelium is regulated by the endosome: Rab4-mediated trafficking and p18-dependent signaling.

Our results suggest that spinal SGK1 phosphorylation, which subsequently triggers the GRASP-1/Rab4 cascade, plays a pivotal role in CFA-induced inflammatory pain by regulating GluR1-containing AMPAR recycling in the dorsal horn.
Title: Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.

The reduction of vesicles exhibiting directional mobility induced by Rab4 mutants indicates that GTP-GDP switching is required for maintaining directional mobility.
Title: Rab4 and Rab5 GTPase are required for directional mobility of endocytic vesicles in astrocytes.

Reduced Akt2 leads to up-regulation of Rab4a expression in cardiomyocytes in a cell-autonomous fashion that may involve activation of PPARalpha.
Title: Akt2 deficiency promotes cardiac induction of Rab4a and myocardial ?-adrenergic hypersensitivity.

corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane
Title: The stress hormone corticosterone increases synaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors via serum- and glucocorticoid-inducible kinase (SGK) regulation of the GDI-Rab4 complex.

The following Rab4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01666	NM_013019.2 Latest version!	Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa43134	XM_008772600.2 Latest version!	Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab4a ( NM_013019.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037151.2 Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), mRNA.

Price & Availability↑

CloneID	ORa01666
Clone ID Related Accession (Same CDS sequence)	NM_013019.2
Accession Version	NM_013019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Rab-4A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062016.1. On Oct 8, 2005 this sequence version replaced NM_013019.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGA CCGCCATGTC CGAGACTTAC GATTTCTTGT TTAAGTTCTT GGTCATTGGA 
AATGCGGGAA CTGGCAAATC CTGCTTGCTC CATCAGTTCA TTGAGAAGAA ATTCAAAGAT 
GACTCAAATC ATACCATAGG AGTGGAATTC GGCTCAAAGA TAATAAATGT TGGTGGTAAA 
TATGTGAAGT TACAGATATG GGACACGGCT GGACAGGAGC GGTTCAGGTC TGTGACGAGA 
AGCTACTACA GAGGTGCGGC TGGGGCACTC CTCGTCTATG ACATCACCAG CCGAGAAACC 
TACAATGCGC TTACTAATTG GTTAACAGAT GCCAGAATGC TGGCGAGCCA GAACATCGTC 
ATCATTCTCT GCGGGAACAA GAAGGACCTG GATGCCGACC GGGAAGTCAC CTTCCTTGAA 
GCCTCCAGGT TCGCACAAGA GAATGAGCTC ATGTTCCTGG AAACCAGTGC ACTGACTGGC 
GAGAACGTCG AAGAGGCTTT CATGCAGTGC GCAAGGAAGA TACTTAACAA AATTGAATCA 
GGTGAGCTGG ACCCCGAGAG GATGGGCTCT GGTATCCAGT ATGGAGACGC CGCCTTGAGA 
CAGCTACGGT CACCCCGACG TACACAGGCT CCAAGTGCAC AGGAGTGTGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037151.2
CDS	123..779
Translation	MAQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFMQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRTQAPSAQECGC

Target ORF information:

RefSeq Version	NM_013019.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013019.2

ORF Insert Sequence:

ATGGCGCAGA CCGCCATGTC CGAGACTTAC GATTTCTTGT TTAAGTTCTT GGTCATTGGA 
AATGCGGGAA CTGGCAAATC CTGCTTGCTC CATCAGTTCA TTGAGAAGAA ATTCAAAGAT 
GACTCAAATC ATACCATAGG AGTGGAATTC GGCTCAAAGA TAATAAATGT TGGTGGTAAA 
TATGTGAAGT TACAGATATG GGACACGGCT GGACAGGAGC GGTTCAGGTC TGTGACGAGA 
AGCTACTACA GAGGTGCGGC TGGGGCACTC CTCGTCTATG ACATCACCAG CCGAGAAACC 
TACAATGCGC TTACTAATTG GTTAACAGAT GCCAGAATGC TGGCGAGCCA GAACATCGTC 
ATCATTCTCT GCGGGAACAA GAAGGACCTG GATGCCGACC GGGAAGTCAC CTTCCTTGAA 
GCCTCCAGGT TCGCACAAGA GAATGAGCTC ATGTTCCTGG AAACCAGTGC ACTGACTGGC 
GAGAACGTCG AAGAGGCTTT CATGCAGTGC GCAAGGAAGA TACTTAACAA AATTGAATCA 
GGTGAGCTGG ACCCCGAGAG GATGGGCTCT GGTATCCAGT ATGGAGACGC CGCCTTGAGA 
CAGCTACGGT CACCCCGACG TACACAGGCT CCAAGTGCAC AGGAGTGTGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab4a ( XM_008772600.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770822.1 Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43134
Clone ID Related Accession (Same CDS sequence)	XM_008772600.2
Accession Version	XM_008772600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Rab-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAC CAGATGCCAG AATGCTGGCG AGCCAGAACA TCGTCATCAT TCTCTGCGGG 
AACAAGAAGG ACCTGGATGC CGACCGGGAA GTCACCTTCC TTGAAGCCTC CAGGTTCGCA 
CAAGAGAATG AGCTCATGTT CCTGGAAACC AGTGCACTGA CTGGCGAGAA CGTCGAAGAG 
GCTTTCATGC AGTGCGCAAG GAAGATACTT AACAAAATTG AATCAGGTGA GCTGGACCCC 
GAGAGGATGG GCTCTGGTAT CCAGTATGGA GACGCCGCCT TGAGACAGCT ACGGTCACCC 
CGACGTACAC AGGCTCCAAG TGCACAGGAG TGTGGCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770822.1
CDS	569..910
Translation	MTSPDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFMQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRTQAPSAQECGC

Target ORF information:

RefSeq Version	XM_008772600.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB4A, member RAS oncogene family (Rab4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772600.2

ORF Insert Sequence:

ATGACATCAC CAGATGCCAG AATGCTGGCG AGCCAGAACA TCGTCATCAT TCTCTGCGGG 
AACAAGAAGG ACCTGGATGC CGACCGGGAA GTCACCTTCC TTGAAGCCTC CAGGTTCGCA 
CAAGAGAATG AGCTCATGTT CCTGGAAACC AGTGCACTGA CTGGCGAGAA CGTCGAAGAG 
GCTTTCATGC AGTGCGCAAG GAAGATACTT AACAAAATTG AATCAGGTGA GCTGGACCCC 
GAGAGGATGG GCTCTGGTAT CCAGTATGGA GACGCCGCCT TGAGACAGCT ACGGTCACCC 
CGACGTACAC AGGCTCCAAG TGCACAGGAG TGTGGCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons.
Neuroscience36193-107(2017 Oct)
Jackson TC,Kotermanski SE,Kochanek PM

Neovascularization in the pulmonary endothelium is regulated by the endosome: Rab4-mediated trafficking and p18-dependent signaling.
American journal of physiology. Lung cellular and molecular physiology309(7)L700-9(2015 Oct)
Chichger H,Braza J,Duong H,Stark M,Harrington EO

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.
The EMBO journal32(5)713-27(2013 Mar)
Kuijl C,Pilli M,Alahari SK,Janssen H,Khoo PS,Ervin KE,Calero M,Jonnalagadda S,Scheller RH,Neefjes J,Junutula JR

Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.
Pain153(12)2380-92(2012 Dec)
Peng HY,Chen GD,Hsieh MC,Lai CY,Huang YP,Lin TB