Pnoc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnoc
Entrez Gene ID	25516
Full Name	prepronociceptin
Synonyms	N23K,Npnc1
General protein information	Preferred Names prepronociceptin Names prepronociceptin Prepronociceptin (neuropeptide nociceptin) (N23K) neuropeptide nociceptin 1 nociceptin orphanin FQ
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p11
Summary	endogenous ligand of the opioid-like orphan receptor; reduces neuronal excitability and induces an increase in inward rectifying K+ conductance in thalamic neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006252199.3	XP_006252261.1	prepronociceptin isoform X2
NM_013007.1	NP_037139.1	prepronociceptin precursor
XM_006252196.3	XP_006252258.1	prepronociceptin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PNOC	NP_006219.1
Canis lupus familiaris (dog)	PNOC	XP_005635726.1
Bos taurus (cattle)	PNOC	NP_776575.1
Mus musculus (house mouse)	Pnoc	NP_001192004.1
Rattus norvegicus (Norway rat)	Pnoc	NP_037139.1
Pan troglodytes (chimpanzee)	PNOC	XP_519684.3
Xenopus tropicalis (tropical clawed frog)	pnoc	NP_001106534.1
	PNOC	XP_001110309.1
Danio rerio (zebrafish)	pnoca	XP_003200377.2

Gene Ontology
Bibliography

Related articles in PubMed

Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system.
Thomas RC, Bath MF, Stover CM, Lambert DG, Thompson JP
Peptides6156-60(2014 Nov)

Nociceptin/orphanin FQ-induced inhibition of delayed rectifier potassium currents by calcium/calmodulin-dependent protein kinase type II.
Wang Y, Yue W, Wang W, Yu J, Liu R, Wang P, Cao Y, Zhang Q, Wang X, Qu L
Neuroreport25(15)1227-31(2014 Oct)

Estradiol negatively modulates the pleiotropic actions of orphanin FQ/nociceptin at proopiomelanocortin synapses.
Borgquist A, Kachani M, Tavitian N, Sinchak K, Wagner EJ
Neuroendocrinology98(1)60-72(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LPS-inoculation in rodents is an unsuitable model for studying possible changes in the NOP-N/OFQ system in sepsis.
Title: Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system.

Results suggest that CaMKII is involved in the inhibitory effect of N/OFQ on delayed rectifier potassium currents in acutely dissociated rat cerebral parietal cortical neurons
Title: Nociceptin/orphanin FQ-induced inhibition of delayed rectifier potassium currents by calcium/calmodulin-dependent protein kinase type II.

Estradiol negatively modulates the pleiotropic inhibitory actions of nociceptin.
Title: Estradiol negatively modulates the pleiotropic actions of orphanin FQ/nociceptin at proopiomelanocortin synapses.

These results suggest that N/OFQ can act directly in the nucleus accumbens shell to block cocaine-induced increases in extracellular dopamine levels
Title: Retrodialysis of N/OFQ into the nucleus accumbens shell blocks cocaine-induced increases in extracellular dopamine and locomotor activity.

The aim of the study was to determine by immunochemistry the expression of leptin, orexin A and orphanin FQ in the major salivary glands (parotid, submandibular and sublingual) of rat, sheep and cow.
Title: Immunohistochemical expression and distribution of orexin, orphanin and leptin in the major salivary glands of some mammals.

The following Pnoc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnoc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12556	NM_013007.1 Latest version!	Rattus norvegicus prepronociceptin (Pnoc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12556	XM_006252199.3 Latest version!	Rattus norvegicus prepronociceptin (Pnoc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa36804	XM_006252196.3 Latest version!	Rattus norvegicus prepronociceptin (Pnoc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnoc ( NM_013007.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037139.1 Rattus norvegicus prepronociceptin (Pnoc), mRNA.

Price & Availability↑

CloneID	ORa12556
Clone ID Related Accession (Same CDS sequence)	XM_006252199.3 , NM_013007.1
Accession Version	NM_013007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	prepronociceptin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X97375.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X97375.1, U48262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGTAAT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037139.1
CDS	25..570
Translation	MKILFCDVLLLSLLSSVFSSCPEDCLTCQERLHPAPGSFNLKLCILQCEEKVFPRPLWTLCTKAMASDSEQLSPADPELTSAALYQSKASEMQHLKRMPRVRSVVQARDAEPEADAEPVADEADEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGNV

Target ORF information:

RefSeq Version	NM_013007.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prepronociceptin (Pnoc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013007.1

ORF Insert Sequence:

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGTAAT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( XM_006252199.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252261.1 Rattus norvegicus prepronociceptin (Pnoc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12556
Clone ID Related Accession (Same CDS sequence)	XM_006252199.3 , NM_013007.1
Accession Version	XM_006252199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prepronociceptin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGTAAT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252261.1
CDS	201..746
Translation	MKILFCDVLLLSLLSSVFSSCPEDCLTCQERLHPAPGSFNLKLCILQCEEKVFPRPLWTLCTKAMASDSEQLSPADPELTSAALYQSKASEMQHLKRMPRVRSVVQARDAEPEADAEPVADEADEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGNV

Target ORF information:

RefSeq Version	XM_006252199.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prepronociceptin (Pnoc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252199.3

ORF Insert Sequence:

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGTAAT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( XM_006252196.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252258.1 Rattus norvegicus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36804
Clone ID Related Accession (Same CDS sequence)	XM_006252196.3
Accession Version	XM_006252196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prepronociceptin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGCATC 
CAGGTGAGCC CCCGTACAGC ATGTGTCCAC ACCAAGACCT GCAGGCCGGG AGTCAGGATT 
CCTCCTTCCC TGAGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252258.1
CDS	205..825
Translation	MKILFCDVLLLSLLSSVFSSCPEDCLTCQERLHPAPGSFNLKLCILQCEEKVFPRPLWTLCTKAMASDSEQLSPADPELTSAALYQSKASEMQHLKRMPRVRSVVQARDAEPEADAEPVADEADEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGIQVSPRTACVHTKTCRPGVRIPPSLRH

Target ORF information:

RefSeq Version	XM_006252196.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252196.3

ORF Insert Sequence:

ATGAAAATCC TGTTTTGTGA TGTCCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCGAGG ACTGCCTCAC CTGCCAGGAG AGGCTCCACC CGGCTCCGGG CAGCTTCAAC 
CTGAAGCTGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTCTT 
TGCACCAAAG CCATGGCCAG TGACTCTGAG CAGCTCAGCC CTGCTGATCC AGAGCTCACG 
TCCGCTGCTC TTTACCAGTC GAAAGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCAGGAGTG TGGTGCAAGC CCGAGACGCA GAGCCTGAGG CAGATGCAGA GCCTGTCGCA 
GATGAGGCCG ATGAGGTGGA GCAGAAGCAG CTGCAGAAAA GGTTTGGGGG CTTCACTGGG 
GCCCGGAAGT CAGCCCGGAA GTTGGCCAAC CAGAAGCGGT TCAGTGAGTT TATGAGGCAG 
TACCTGGTCC TGAGCATGCA GTCAAGCCAA CGCCGGCGCA CTCTGCACCA GAATGGCATC 
CAGGTGAGCC CCCGTACAGC ATGTGTCCAC ACCAAGACCT GCAGGCCGGG AGTCAGGATT 
CCTCCTTCCC TGAGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system.
Peptides6156-60(2014 Nov)
Thomas RC,Bath MF,Stover CM,Lambert DG,Thompson JP

Nociceptin/orphanin FQ-induced inhibition of delayed rectifier potassium currents by calcium/calmodulin-dependent protein kinase type II.
Neuroreport25(15)1227-31(2014 Oct)
Wang Y,Yue W,Wang W,Yu J,Liu R,Wang P,Cao Y,Zhang Q,Wang X,Qu L

Estradiol negatively modulates the pleiotropic actions of orphanin FQ/nociceptin at proopiomelanocortin synapses.
Neuroendocrinology98(1)60-72(2013)
Borgquist A,Kachani M,Tavitian N,Sinchak K,Wagner EJ