Hbegf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hbegf
Entrez Gene ID	25433
Full Name	heparin-binding EGF-like growth factor
Synonyms	Dtr,GFHB,Hb-egf,Hegfl
General protein information	Preferred Names heparin-binding EGF-like growth factor Names proheparin-binding EGF-like growth factor Diphtheria toxin receptor (heparin binding epidermal growth factor - like growth factor) heparin binding epidermal growth factor-like growth factor heparin-binding epidermal -growth - like growth factor heparin-binding epidermal -growth factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11
Summary	activates epidermal growth factor receptor mediated signaling; contributes to neuronal survival; may play a role in neurogenesis during ventral midbrain development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012945.1	NP_037077.1	proheparin-binding EGF-like growth factor precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HBEGF	XP_001137119.1
	HBEGF	XP_001085848.1
Canis lupus familiaris (dog)	HBEGF	XP_005617336.1
Homo sapiens (human)	HBEGF	NP_001936.1
Bos taurus (cattle)	HBEGF	NP_001137562.1
Rattus norvegicus (Norway rat)	Hbegf	NP_037077.1
Gallus gallus (chicken)	HBEGF	NP_990180.1
Mus musculus (house mouse)	Hbegf	NP_034545.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterotrimeric G proteins directly regulate MMP14/membrane type-1 matrix metalloprotease: a novel mechanism for GPCR-EGFR transactivation.
Overland AC, Insel PA
The Journal of biological chemistry290(16)9941-7(2015 Apr)

Heparin-binding EGF-like growth factor (HB-EGF) promotes cell migration and adhesion via focal adhesion kinase.
Su Y, Besner GE
The Journal of surgical research189(2)222-31(2014 Jun)

Heparin-binding epidermal growth factor-like growth factor: a hepatic stellate cell proliferation inducer via ErbB receptors.
Zhang D, Zhang J, Jiang X, Li X, Wang Y, Ma J, Jiang H
Journal of gastroenterology and hepatology29(3)623-32(2014 Mar)

CAP37 activation of PKC promotes human corneal epithelial cell chemotaxis.
Griffith GL, Russell RA, Kasus-Jacobi A, Thavathiru E, Gonzalez ML, Logan S, Pereira HA
Investigative ophthalmology & visual science54(10)6712-23(2013 Oct)

Heparin-binding epidermal growth factor-like growth factor protects rat intestine after portal triad clamping.
Liao X, Chen L, Fu W, Zhou J
Growth factors (Chur, Switzerland)31(2)74-80(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Heterotrimeric G proteins directly regulate membrane-localized MMP14/MT1-MMP, resulting in HB-EGF release and EGFR transactivation.
Title: Heterotrimeric G proteins directly regulate MMP14/membrane type-1 matrix metalloprotease: a novel mechanism for GPCR-EGFR transactivation.

FAK phosphorylation and FAK-mediated signal transduction play essential roles in HB-EGF-mediated intestinal epithelial cell migration and adhesion.
Title: Heparin-binding EGF-like growth factor (HB-EGF) promotes cell migration and adhesion via focal adhesion kinase.

HB-EGF promotes hepatic stellate cell proliferation via activation of the EGFR and ErbB4 receptors
Title: Heparin-binding epidermal growth factor-like growth factor: a hepatic stellate cell proliferation inducer via ErbB receptors.

Intraluminal administration of HB-EGF reduces the severity of intestinal congestion/repurfusion(C/R) injury in rats.
Title: Heparin-binding epidermal growth factor-like growth factor protects rat intestine after portal triad clamping.

HB-EGF induces cardiomyocyte hypertrophy through an EGFR-ERK5-MEF2A-COX-2 pathway.
Title: HB-EGF induces cardiomyocyte hypertrophy via an ERK5-MEF2A-COX2 signaling pathway.

The following Hbegf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hbegf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01402	NM_012945.1 Latest version!	Rattus norvegicus heparin-binding EGF-like growth factor (Hbegf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hbegf ( NM_012945.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037077.1 Rattus norvegicus heparin-binding EGF-like growth factor (Hbegf), mRNA.

Price & Availability↑

CloneID	ORa01402
Clone ID Related Accession (Same CDS sequence)	NM_012945.1
Accession Version	NM_012945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	proheparin-binding EGF-like growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L05489.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L05489.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGC TGCCGTCGGT GGTGCTGAAG CTCTTTCTGG CCGCAGTGTT GTCCGCGTTG 
GTGACCGGTG AGAGTCTGGA GCGGCTTCGG AGAGGTCTGG CGGCAGCAAC CAGCAACCCT 
GACCCTCCCA CTGGAACCAC AAACCAGCTG CTACCCACGG GAGCTGATCG CGCTCAGGAG 
GTCCAGGACT TGGAAGGGAC CGATCTGGAC CTTTTCAAAG TTGCTTTCTC CTCCAAGCCA 
CAAGCCCTGG CCACCCCAGG AAAAGAAAAG AACGGGAAAA AGAAGAGGAA AGGCAAGGGG 
TTAGGAAAGA AGAGAGATCC ATGCCTTAAG AAATACAAGG ACTACTGCAT CCACGGAGAG 
TGCAGATACC TGAAGGAGCT CCGTATTCCC TCGTGCCACT GCCTCCCTGG TTACCATGGA 
CAGAGGTGTC ATGGGCTGAC CCTACCGGTA GAGAACCCCC TGTACACATA TGACCACACT 
ACCGTCTTGG CTGTGGTGGC TGTAGTACTG TCATCTGTCT GTCTTCTTGT CATCGTGGGA 
CTTCTCATGT TCAGGTACCA TAGGCGAGGA GGTTATGACT TGGAAAGTGA GGAGAAAGTG 
AAGTTGGGCA TGGCTAGCTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037077.1
CDS	32..658
Translation	MKLLPSVVLKLFLAAVLSALVTGESLERLRRGLAAATSNPDPPTGTTNQLLPTGADRAQEVQDLEGTDLDLFKVAFSSKPQALATPGKEKNGKKKRKGKGLGKKRDPCLKKYKDYCIHGECRYLKELRIPSCHCLPGYHGQRCHGLTLPVENPLYTYDHTTVLAVVAVVLSSVCLLVIVGLLMFRYHRRGGYDLESEEKVKLGMASSH

Target ORF information:

RefSeq Version	NM_012945.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heparin-binding EGF-like growth factor (Hbegf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012945.1

ORF Insert Sequence:

ATGAAGCTGC TGCCGTCGGT GGTGCTGAAG CTCTTTCTGG CCGCAGTGTT GTCCGCGTTG 
GTGACCGGTG AGAGTCTGGA GCGGCTTCGG AGAGGTCTGG CGGCAGCAAC CAGCAACCCT 
GACCCTCCCA CTGGAACCAC AAACCAGCTG CTACCCACGG GAGCTGATCG CGCTCAGGAG 
GTCCAGGACT TGGAAGGGAC CGATCTGGAC CTTTTCAAAG TTGCTTTCTC CTCCAAGCCA 
CAAGCCCTGG CCACCCCAGG AAAAGAAAAG AACGGGAAAA AGAAGAGGAA AGGCAAGGGG 
TTAGGAAAGA AGAGAGATCC ATGCCTTAAG AAATACAAGG ACTACTGCAT CCACGGAGAG 
TGCAGATACC TGAAGGAGCT CCGTATTCCC TCGTGCCACT GCCTCCCTGG TTACCATGGA 
CAGAGGTGTC ATGGGCTGAC CCTACCGGTA GAGAACCCCC TGTACACATA TGACCACACT 
ACCGTCTTGG CTGTGGTGGC TGTAGTACTG TCATCTGTCT GTCTTCTTGT CATCGTGGGA 
CTTCTCATGT TCAGGTACCA TAGGCGAGGA GGTTATGACT TGGAAAGTGA GGAGAAAGTG 
AAGTTGGGCA TGGCTAGCTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterotrimeric G proteins directly regulate MMP14/membrane type-1 matrix metalloprotease: a novel mechanism for GPCR-EGFR transactivation.
The Journal of biological chemistry290(16)9941-7(2015 Apr)
Overland AC,Insel PA

Heparin-binding EGF-like growth factor (HB-EGF) promotes cell migration and adhesion via focal adhesion kinase.
The Journal of surgical research189(2)222-31(2014 Jun)
Su Y,Besner GE

Heparin-binding epidermal growth factor-like growth factor: a hepatic stellate cell proliferation inducer via ErbB receptors.
Journal of gastroenterology and hepatology29(3)623-32(2014 Mar)
Zhang D,Zhang J,Jiang X,Li X,Wang Y,Ma J,Jiang H

CAP37 activation of PKC promotes human corneal epithelial cell chemotaxis.
Investigative ophthalmology & visual science54(10)6712-23(2013 Oct)
Griffith GL,Russell RA,Kasus-Jacobi A,Thavathiru E,Gonzalez ML,Logan S,Pereira HA

Heparin-binding epidermal growth factor-like growth factor protects rat intestine after portal triad clamping.
Growth factors (Chur, Switzerland)31(2)74-80(2013 Apr)
Liao X,Chen L,Fu W,Zhou J