Dpysl3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpysl3
Entrez Gene ID	25418
Full Name	dihydropyrimidinase-like 3
Synonyms	Crmp4,TUC-4b
General protein information	Preferred Names dihydropyrimidinase-like 3 Names dihydropyrimidinase-related protein 3 CRMP-4 DRP-3 TOAD-64/Ulip/CRMP protein 4b collapsin response mediator protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11-q11
Summary	protein expressed in the nervous system during development that may be involved in neuronal plasticity, neurite growth and extension, and may serve as a ligand of the extracellular matrix during brain development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012934.1	NP_037066.1	dihydropyrimidinase-related protein 3
XM_006254670.3	XP_006254732.1	dihydropyrimidinase-related protein 3 isoform X1
XM_006254671.3	XP_006254733.1	dihydropyrimidinase-related protein 3 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DPYSL3	NP_989824.1
Danio rerio (zebrafish)	dpysl3	NP_001018348.1
Mus musculus (house mouse)	Dpysl3	NP_033494.1
Xenopus tropicalis (tropical clawed frog)	dpysl3	NP_001005637.1
Homo sapiens (human)	DPYSL3	NP_001184223.1
Pan troglodytes (chimpanzee)	DPYSL3	XP_003310939.1
	DPYSL3	XP_001102251.1
Bos taurus (cattle)	DPYSL3	NP_001094538.1
Rattus norvegicus (Norway rat)	Dpysl3	NP_037066.1
Canis lupus familiaris (dog)	DPYSL3	XP_544332.3

Gene Ontology
Bibliography

Related articles in PubMed

CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons.
Cha C, Zhang J, Ji Z, Tan M, Li S, Wu F, Chen K, Gong S, Guo G, Lin H
Brain research bulletin124286-94(2016 Jun)

CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation.
Tan M, Cha C, Ye Y, Zhang J, Li S, Wu F, Gong S, Guo G
Neural plasticity2015947423(2015)

Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling.
Nakamura F, Kumeta K, Hida T, Isono T, Nakayama Y, Kuramata-Matsuoka E, Yamashita N, Uchida Y, Ogura K, Gengyo-Ando K, Mitani S, Ogino T, Goshima Y
Nature communications55325(2014 Oct)

Dihydropyrimidinase-like 3 regulates the inflammatory response of activated microglia.
Manivannan J, Tay SS, Ling EA, Dheen ST
Neuroscience25340-54(2013 Dec)

Injury-induced CRMP4 expression in adult sensory neurons; a possible target gene for ciliary neurotrophic factor.
Jang SY, Shin YK, Jung J, Lee SH, Seo SY, Suh DJ, Park HT
Neuroscience letters485(1)37-42(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study suggest that CRMP4 is involved in dendritic development via the interaction with actin cytoskeleton in hippocampal neurons.
Title: CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons.

Data suggest collapsin response mediator proteins (CRMP) CRMP2 and CRMP4 form complexes to bridge microtubules and actin and work cooperatively to regulate growth cone development and axonal elongation.
Title: CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation.

The results of this study suggested that knockdown of Dpysl3 can inhibit activation, migration and phagocytic capability of microglia and consequently reduce neuroinflammation.
Title: Dihydropyrimidinase-like 3 regulates the inflammatory response of activated microglia.

CRMP4 may be a target gene for CNTF in the regenerative axon growth of DRG neurons after injury.
Title: Injury-induced CRMP4 expression in adult sensory neurons; a possible target gene for ciliary neurotrophic factor.

The findings demonstrated that calpain-truncated CRMP-3 and -4 act as pro-apoptotic players when CGNs undergo apoptosis.
Title: Calpain-truncated CRMP-3 and -4 contribute to potassium deprivation-induced apoptosis of cerebellar granule neurons.

The following Dpysl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpysl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01366	NM_012934.1 Latest version!	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38353	XM_006254671.3 Latest version!	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38352	XM_006254670.3 Latest version!	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpysl3 ( NM_012934.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037066.1 Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), mRNA.

Price & Availability↑

CloneID	ORa01366
Clone ID Related Accession (Same CDS sequence)	NM_012934.1
Accession Version	NM_012934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	dihydropyrimidinase-related protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF389425.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF389425.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTACC AGGGCAAGAA GAACATTCCT CGGATCACGA GTGACCGTCT TCTAATCAAG 
GGAGGGAGAA TCGTCAACGA TGATCAGTCC TTTTATGCTG ATATTTACAT GGAGGATGGC 
TTGATAAAGC AAATTGGAGA CAATCTGATT GTCCCTGGAG GTGTGAAGAC CATTGAGGCC 
AATGGGAAGA TGGTGATGCC TGGAGGCATT GATGTCCATA CCCACTTCCA GATGCCTTAC 
AAGGGGATGA CCACAGTGGA CGATTTCTTC CAAGGGACAA AGGCTGCCTT AGCGGGAGGA 
ACCACCATGA TCATTGACCA TGTGGTACCT GAACCTGAGT CTAGCCTGAC CGAGGCCTAT 
GAAAAGTGGC GTGAGTGGGC TGACGGGAAG AGCTGCTGTG ACTATGCTTT GCATGTGGAC 
ATCACCCACT GGAATGACAG CGTCAAGCAA GAGGTGCAGA ACCTCAGTAA GGAAAAAGGC 
GTTAACTCCT TCATGGTTTA CATGGCATAC AAGGATTTAT ATCAAGTGTC CAACACAGAG 
CTCTATGAGA TCTTCACCTG CCTGGGAGAA CTGGGGGCCA TTGCTCAAGT TCATGCCGAG 
AATGGAGACA TCATTGCCCA GGAGCAGGCA CGAATGCTGG AAATGGGAAT AACAGGCCCA 
GAAGGTCATG TTCTGAGCAG ACCGGAAGAG CTGGAAGCTG AGGCTGTGTT CCGTGCCATC 
ACCGTCGCCA GCCAGACCAA CTGCCCCCTT TATGTCACCA AGGTCATGAG CAAGAGCGCG 
GCTGATCTCA TCTCACAAGC CAGGAAGAAA GGAAATGTGG TCTTTGGCGA GCCCATCACT 
GCCAGCCTGG GAATAGATGG AACCCATTAC TGGAGTAAGA ACTGGGCCAA GGCAGCTGCA 
TTTGTGACAT CCCCACCTCT GAGCCCTGAC CCAACCACAC CTGACTACAT CAACTCCTTG 
CTGGCCAGTG GAGATCTGCA GCTCTCTGGA AGTGCCCACT GTACCTTCAG CACTGCCCAG 
AAAGCCATTG GGAAGGACAA CTTCACGGCT ATCCCTGAGG GCACCAATGG CGTGGAGGAG 
CGTATGTCTG TCATCTGGGA CAAGGCTGTG GCCACAGGGA AGATGGATGA AAACCAGTTT 
GTGGCTGTGA CAAGTACCAA CGCTGCCAAG ATATTCAACC TGTACCCTCG CAAGGGGAGA 
ATAGCTGTGG GTTCTGACAG CGACCTTGTC ATCTGGGATC CAGATGCCGT GAAGATCGTC 
TCTGCCAAGA ACCACCAGTC GGTTGCGGAA TACAACATCT TTGAAGGGAT GGAGCTGCGT 
GGGGCACCTC TGGTGGTTAT CTGCCAGGGC AAGATCATGC TGGAAGATGG TAACCTGCAC 
GTGACCCAGG GGGCCGGCCG CTTCATTCCC TGCAGCCCAT TCTCTGACTA TGTCTATAAG 
CGCATTAAAG CAAGGAGGAA GATGGCGGAC CTGCATGCAG TCCCAAGAGG CATGTATGAT 
GGACCAGTGT TTGACTTGAC CACCACCCCC AAGGGGGGCA CCCCAGCTGG CTCTACTCGA 
GGCTCTCCCA CTCGGCCAAA CCCACCAGTG AGGAACCTCC ATCAGTCGGG ATTTAGTCTG 
TCAGGCACCC AAGTGGATGA GGGTGTCCGC TCAGCCAGCA AACGCATTGT GGCGCCCCCT 
GGAGGCCGTT CTAACATCAC ATCCCTGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037066.1
CDS	80..1792
Translation	MSYQGKKNIPRITSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVMPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLSKEKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQARMLEMGITGPEGHVLSRPEELEAEAVFRAITVASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDSDLVIWDPDAVKIVSAKNHQSVAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSTRGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS

Target ORF information:

RefSeq Version	NM_012934.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012934.1

ORF Insert Sequence:

ATGTCCTACC AGGGCAAGAA GAACATTCCT CGGATCACGA GTGACCGTCT TCTAATCAAG 
GGAGGGAGAA TCGTCAACGA TGATCAGTCC TTTTATGCTG ATATTTACAT GGAGGATGGC 
TTGATAAAGC AAATTGGAGA CAATCTGATT GTCCCTGGAG GTGTGAAGAC CATTGAGGCC 
AATGGGAAGA TGGTGATGCC TGGAGGCATT GATGTCCATA CCCACTTCCA GATGCCTTAC 
AAGGGGATGA CCACAGTGGA CGATTTCTTC CAAGGGACAA AGGCTGCCTT AGCGGGAGGA 
ACCACCATGA TCATTGACCA TGTGGTACCT GAACCTGAGT CTAGCCTGAC CGAGGCCTAT 
GAAAAGTGGC GTGAGTGGGC TGACGGGAAG AGCTGCTGTG ACTATGCTTT GCATGTGGAC 
ATCACCCACT GGAATGACAG CGTCAAGCAA GAGGTGCAGA ACCTCAGTAA GGAAAAAGGC 
GTTAACTCCT TCATGGTTTA CATGGCATAC AAGGATTTAT ATCAAGTGTC CAACACAGAG 
CTCTATGAGA TCTTCACCTG CCTGGGAGAA CTGGGGGCCA TTGCTCAAGT TCATGCCGAG 
AATGGAGACA TCATTGCCCA GGAGCAGGCA CGAATGCTGG AAATGGGAAT AACAGGCCCA 
GAAGGTCATG TTCTGAGCAG ACCGGAAGAG CTGGAAGCTG AGGCTGTGTT CCGTGCCATC 
ACCGTCGCCA GCCAGACCAA CTGCCCCCTT TATGTCACCA AGGTCATGAG CAAGAGCGCG 
GCTGATCTCA TCTCACAAGC CAGGAAGAAA GGAAATGTGG TCTTTGGCGA GCCCATCACT 
GCCAGCCTGG GAATAGATGG AACCCATTAC TGGAGTAAGA ACTGGGCCAA GGCAGCTGCA 
TTTGTGACAT CCCCACCTCT GAGCCCTGAC CCAACCACAC CTGACTACAT CAACTCCTTG 
CTGGCCAGTG GAGATCTGCA GCTCTCTGGA AGTGCCCACT GTACCTTCAG CACTGCCCAG 
AAAGCCATTG GGAAGGACAA CTTCACGGCT ATCCCTGAGG GCACCAATGG CGTGGAGGAG 
CGTATGTCTG TCATCTGGGA CAAGGCTGTG GCCACAGGGA AGATGGATGA AAACCAGTTT 
GTGGCTGTGA CAAGTACCAA CGCTGCCAAG ATATTCAACC TGTACCCTCG CAAGGGGAGA 
ATAGCTGTGG GTTCTGACAG CGACCTTGTC ATCTGGGATC CAGATGCCGT GAAGATCGTC 
TCTGCCAAGA ACCACCAGTC GGTTGCGGAA TACAACATCT TTGAAGGGAT GGAGCTGCGT 
GGGGCACCTC TGGTGGTTAT CTGCCAGGGC AAGATCATGC TGGAAGATGG TAACCTGCAC 
GTGACCCAGG GGGCCGGCCG CTTCATTCCC TGCAGCCCAT TCTCTGACTA TGTCTATAAG 
CGCATTAAAG CAAGGAGGAA GATGGCGGAC CTGCATGCAG TCCCAAGAGG CATGTATGAT 
GGACCAGTGT TTGACTTGAC CACCACCCCC AAGGGGGGCA CCCCAGCTGG CTCTACTCGA 
GGCTCTCCCA CTCGGCCAAA CCCACCAGTG AGGAACCTCC ATCAGTCGGG ATTTAGTCTG 
TCAGGCACCC AAGTGGATGA GGGTGTCCGC TCAGCCAGCA AACGCATTGT GGCGCCCCCT 
GGAGGCCGTT CTAACATCAC ATCCCTGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpysl3 ( XM_006254671.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254733.1 Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38353
Clone ID Related Accession (Same CDS sequence)	XM_006254671.3
Accession Version	XM_006254671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dihydropyrimidinase-related protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTCAC CAGCAAGAAA CAAAGTCCGG CAGAGTGACC GTCTTCTAAT CAAGGGAGGG 
AGAATCGTCA ACGATGATCA GTCCTTTTAT GCTGATATTT ACATGGAGGA TGGCTTGATA 
AAGCAAATTG GAGACAATCT GATTGTCCCT GGAGGTGTGA AGACCATTGA GGCCAATGGG 
AAGATGGTGA TGCCTGGAGG CATTGATGTC CATACCCACT TCCAGATGCC TTACAAGGGG 
ATGACCACAG TGGACGATTT CTTCCAAGGG ACAAAGGCTG CCTTAGCGGG AGGAACCACC 
ATGATCATTG ACCATGTGGT ACCTGAACCT GAGTCTAGCC TGACCGAGGC CTATGAAAAG 
TGGCGTGAGT GGGCTGACGG GAAGAGCTGC TGTGACTATG CTTTGCATGT GGACATCACC 
CACTGGAATG ACAGCGTCAA GCAAGAGGTG CAGAACCTCA GTAAGGAAAA AGGCGTTAAC 
TCCTTCATGG TTTACATGGC ATACAAGGAT TTATATCAAG TGTCCAACAC AGAGCTCTAT 
GAGATCTTCA CCTGCCTGGG AGAACTGGGG GCCATTGCTC AAGTTCATGC CGAGAATGGA 
GACATCATTG CCCAGGAGCA GGCACGAATG CTGGAAATGG GAATAACAGG CCCAGAAGGT 
CATGTTCTGA GCAGACCGGA AGAGCTGGAA GCTGAGGCTG TGTTCCGTGC CATCACCGTC 
GCCAGCCAGA CCAACTGCCC CCTTTATGTC ACCAAGGTCA TGAGCAAGAG CGCGGCTGAT 
CTCATCTCAC AAGCCAGGAA GAAAGGAAAT GTGGTCTTTG GCGAGCCCAT CACTGCCAGC 
CTGGGAATAG ATGGAACCCA TTACTGGAGT AAGAACTGGG CCAAGGCAGC TGCATTTGTG 
ACATCCCCAC CTCTGAGCCC TGACCCAACC ACACCTGACT ACATCAACTC CTTGCTGGCC 
AGTGGAGATC TGCAGCTCTC TGGAAGTGCC CACTGTACCT TCAGCACTGC CCAGAAAGCC 
ATTGGGAAGG ACAACTTCAC GGCTATCCCT GAGGGCACCA ATGGCGTGGA GGAGCGTATG 
TCTGTCATCT GGGACAAGGC TGTGGCCACA GGGAAGATGG ATGAAAACCA GTTTGTGGCT 
GTGACAAGTA CCAACGCTGC CAAGATATTC AACCTGTACC CTCGCAAGGG GAGAATAGCT 
GTGGGTTCTG ACAGCGACCT TGTCATCTGG GATCCAGATG CCGTGAAGAT CGTCTCTGCC 
AAGAACCACC AGTCGGTTGC GGAATACAAC ATCTTTGAAG GGATGGAGCT GCGTGGGGCA 
CCTCTGGTGG TTATCTGCCA GGGCAAGATC ATGCTGGAAG ATGGTAACCT GCACGTGACC 
CAGGGGGCCG GCCGCTTCAT TCCCTGCAGC CCATTCTCTG ACTATGTCTA TAAGCGCATT 
AAAGCAAGGA GGAAGATGGC GGACCTGCAT GCAGTCCCAA GAGGCATGTA TGATGGACCA 
GTGTTTGACT TGACCACCAC CCCCAAGGGG GGCACCCCAG CTGGCTCTAC TCGAGGCTCT 
CCCACTCGGC CAAACCCACC AGTGAGGAAC CTCCATCAGT CGGGATTTAG TCTGTCAGGC 
ACCCAAGTGG ATGAGGGTGT CCGCTCAGCC AGCAAACGCA TTGTGGCGCC CCCTGGAGGC 
CGTTCTAACA TCACATCCCT GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254733.1
CDS	271..1977
Translation	MYSPARNKVRQSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVMPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLSKEKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQARMLEMGITGPEGHVLSRPEELEAEAVFRAITVASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDSDLVIWDPDAVKIVSAKNHQSVAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSTRGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS

Target ORF information:

RefSeq Version	XM_006254671.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254671.3

ORF Insert Sequence:

ATGTATTCAC CAGCAAGAAA CAAAGTCCGG CAGAGTGACC GTCTTCTAAT CAAGGGAGGG 
AGAATCGTCA ACGATGATCA GTCCTTTTAT GCTGATATTT ACATGGAGGA TGGCTTGATA 
AAGCAAATTG GAGACAATCT GATTGTCCCT GGAGGTGTGA AGACCATTGA GGCCAATGGG 
AAGATGGTGA TGCCTGGAGG CATTGATGTC CATACCCACT TCCAGATGCC TTACAAGGGG 
ATGACCACAG TGGACGATTT CTTCCAAGGG ACAAAGGCTG CCTTAGCGGG AGGAACCACC 
ATGATCATTG ACCATGTGGT ACCTGAACCT GAGTCTAGCC TGACCGAGGC CTATGAAAAG 
TGGCGTGAGT GGGCTGACGG GAAGAGCTGC TGTGACTATG CTTTGCATGT GGACATCACC 
CACTGGAATG ACAGCGTCAA GCAAGAGGTG CAGAACCTCA GTAAGGAAAA AGGCGTTAAC 
TCCTTCATGG TTTACATGGC ATACAAGGAT TTATATCAAG TGTCCAACAC AGAGCTCTAT 
GAGATCTTCA CCTGCCTGGG AGAACTGGGG GCCATTGCTC AAGTTCATGC CGAGAATGGA 
GACATCATTG CCCAGGAGCA GGCACGAATG CTGGAAATGG GAATAACAGG CCCAGAAGGT 
CATGTTCTGA GCAGACCGGA AGAGCTGGAA GCTGAGGCTG TGTTCCGTGC CATCACCGTC 
GCCAGCCAGA CCAACTGCCC CCTTTATGTC ACCAAGGTCA TGAGCAAGAG CGCGGCTGAT 
CTCATCTCAC AAGCCAGGAA GAAAGGAAAT GTGGTCTTTG GCGAGCCCAT CACTGCCAGC 
CTGGGAATAG ATGGAACCCA TTACTGGAGT AAGAACTGGG CCAAGGCAGC TGCATTTGTG 
ACATCCCCAC CTCTGAGCCC TGACCCAACC ACACCTGACT ACATCAACTC CTTGCTGGCC 
AGTGGAGATC TGCAGCTCTC TGGAAGTGCC CACTGTACCT TCAGCACTGC CCAGAAAGCC 
ATTGGGAAGG ACAACTTCAC GGCTATCCCT GAGGGCACCA ATGGCGTGGA GGAGCGTATG 
TCTGTCATCT GGGACAAGGC TGTGGCCACA GGGAAGATGG ATGAAAACCA GTTTGTGGCT 
GTGACAAGTA CCAACGCTGC CAAGATATTC AACCTGTACC CTCGCAAGGG GAGAATAGCT 
GTGGGTTCTG ACAGCGACCT TGTCATCTGG GATCCAGATG CCGTGAAGAT CGTCTCTGCC 
AAGAACCACC AGTCGGTTGC GGAATACAAC ATCTTTGAAG GGATGGAGCT GCGTGGGGCA 
CCTCTGGTGG TTATCTGCCA GGGCAAGATC ATGCTGGAAG ATGGTAACCT GCACGTGACC 
CAGGGGGCCG GCCGCTTCAT TCCCTGCAGC CCATTCTCTG ACTATGTCTA TAAGCGCATT 
AAAGCAAGGA GGAAGATGGC GGACCTGCAT GCAGTCCCAA GAGGCATGTA TGATGGACCA 
GTGTTTGACT TGACCACCAC CCCCAAGGGG GGCACCCCAG CTGGCTCTAC TCGAGGCTCT 
CCCACTCGGC CAAACCCACC AGTGAGGAAC CTCCATCAGT CGGGATTTAG TCTGTCAGGC 
ACCCAAGTGG ATGAGGGTGT CCGCTCAGCC AGCAAACGCA TTGTGGCGCC CCCTGGAGGC 
CGTTCTAACA TCACATCCCT GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpysl3 ( XM_006254670.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254732.1 Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38352
Clone ID Related Accession (Same CDS sequence)	XM_006254670.3
Accession Version	XM_006254670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dihydropyrimidinase-related protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCGAAGGGG TTGGGACAGC TCCCACGAGG ACGACCTGCC TGTGTACTTG 
GCCCGGCCGG GCACCACAGA TCAGGTCCCA CGGCAGAAGT ACGGTGGCAT GTTCTGCAAC 
GTGGAGGGCG CCTTCGAGAG CAAGACATTG GATTTTGATG CCCTGAGTGT GGGACAGCGC 
GGCGCCAAAA CTCCCCGGAG CAGCCAGGGC AGCGGCCGCG GCGCGGGGAA CCGGCCCGGG 
GTGGAAGGGG ACACGCGCAG GGGCCCGGGC CGGGAGGAGA GCAGGGAGCC CGTGCCTGAG 
TCGCCCAAGC CCGCCGGGGT AGAGATCCGG AGCGCCACTG GCAAGGAGGT CTTGCAGAAC 
CTCGGTCCCA AGGACAAGAG TGACCGTCTT CTAATCAAGG GAGGGAGAAT CGTCAACGAT 
GATCAGTCCT TTTATGCTGA TATTTACATG GAGGATGGCT TGATAAAGCA AATTGGAGAC 
AATCTGATTG TCCCTGGAGG TGTGAAGACC ATTGAGGCCA ATGGGAAGAT GGTGATGCCT 
GGAGGCATTG ATGTCCATAC CCACTTCCAG ATGCCTTACA AGGGGATGAC CACAGTGGAC 
GATTTCTTCC AAGGGACAAA GGCTGCCTTA GCGGGAGGAA CCACCATGAT CATTGACCAT 
GTGGTACCTG AACCTGAGTC TAGCCTGACC GAGGCCTATG AAAAGTGGCG TGAGTGGGCT 
GACGGGAAGA GCTGCTGTGA CTATGCTTTG CATGTGGACA TCACCCACTG GAATGACAGC 
GTCAAGCAAG AGGTGCAGAA CCTCAGTAAG GAAAAAGGCG TTAACTCCTT CATGGTTTAC 
ATGGCATACA AGGATTTATA TCAAGTGTCC AACACAGAGC TCTATGAGAT CTTCACCTGC 
CTGGGAGAAC TGGGGGCCAT TGCTCAAGTT CATGCCGAGA ATGGAGACAT CATTGCCCAG 
GAGCAGGCAC GAATGCTGGA AATGGGAATA ACAGGCCCAG AAGGTCATGT TCTGAGCAGA 
CCGGAAGAGC TGGAAGCTGA GGCTGTGTTC CGTGCCATCA CCGTCGCCAG CCAGACCAAC 
TGCCCCCTTT ATGTCACCAA GGTCATGAGC AAGAGCGCGG CTGATCTCAT CTCACAAGCC 
AGGAAGAAAG GAAATGTGGT CTTTGGCGAG CCCATCACTG CCAGCCTGGG AATAGATGGA 
ACCCATTACT GGAGTAAGAA CTGGGCCAAG GCAGCTGCAT TTGTGACATC CCCACCTCTG 
AGCCCTGACC CAACCACACC TGACTACATC AACTCCTTGC TGGCCAGTGG AGATCTGCAG 
CTCTCTGGAA GTGCCCACTG TACCTTCAGC ACTGCCCAGA AAGCCATTGG GAAGGACAAC 
TTCACGGCTA TCCCTGAGGG CACCAATGGC GTGGAGGAGC GTATGTCTGT CATCTGGGAC 
AAGGCTGTGG CCACAGGGAA GATGGATGAA AACCAGTTTG TGGCTGTGAC AAGTACCAAC 
GCTGCCAAGA TATTCAACCT GTACCCTCGC AAGGGGAGAA TAGCTGTGGG TTCTGACAGC 
GACCTTGTCA TCTGGGATCC AGATGCCGTG AAGATCGTCT CTGCCAAGAA CCACCAGTCG 
GTTGCGGAAT ACAACATCTT TGAAGGGATG GAGCTGCGTG GGGCACCTCT GGTGGTTATC 
TGCCAGGGCA AGATCATGCT GGAAGATGGT AACCTGCACG TGACCCAGGG GGCCGGCCGC 
TTCATTCCCT GCAGCCCATT CTCTGACTAT GTCTATAAGC GCATTAAAGC AAGGAGGAAG 
ATGGCGGACC TGCATGCAGT CCCAAGAGGC ATGTATGATG GACCAGTGTT TGACTTGACC 
ACCACCCCCA AGGGGGGCAC CCCAGCTGGC TCTACTCGAG GCTCTCCCAC TCGGCCAAAC 
CCACCAGTGA GGAACCTCCA TCAGTCGGGA TTTAGTCTGT CAGGCACCCA AGTGGATGAG 
GGTGTCCGCT CAGCCAGCAA ACGCATTGTG GCGCCCCCTG GAGGCCGTTC TAACATCACA 
TCCCTGAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254732.1
CDS	321..2372
Translation	MASGRRGWDSSHEDDLPVYLARPGTTDQVPRQKYGGMFCNVEGAFESKTLDFDALSVGQRGAKTPRSSQGSGRGAGNRPGVEGDTRRGPGREESREPVPESPKPAGVEIRSATGKEVLQNLGPKDKSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVMPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLSKEKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQARMLEMGITGPEGHVLSRPEELEAEAVFRAITVASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDSDLVIWDPDAVKIVSAKNHQSVAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSTRGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS

Target ORF information:

RefSeq Version	XM_006254670.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dihydropyrimidinase-like 3 (Dpysl3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254670.3

ORF Insert Sequence:

ATGGCTTCGG GCCGAAGGGG TTGGGACAGC TCCCACGAGG ACGACCTGCC TGTGTACTTG 
GCCCGGCCGG GCACCACAGA TCAGGTCCCA CGGCAGAAGT ACGGTGGCAT GTTCTGCAAC 
GTGGAGGGCG CCTTCGAGAG CAAGACATTG GATTTTGATG CCCTGAGTGT GGGACAGCGC 
GGCGCCAAAA CTCCCCGGAG CAGCCAGGGC AGCGGCCGCG GCGCGGGGAA CCGGCCCGGG 
GTGGAAGGGG ACACGCGCAG GGGCCCGGGC CGGGAGGAGA GCAGGGAGCC CGTGCCTGAG 
TCGCCCAAGC CCGCCGGGGT AGAGATCCGG AGCGCCACTG GCAAGGAGGT CTTGCAGAAC 
CTCGGTCCCA AGGACAAGAG TGACCGTCTT CTAATCAAGG GAGGGAGAAT CGTCAACGAT 
GATCAGTCCT TTTATGCTGA TATTTACATG GAGGATGGCT TGATAAAGCA AATTGGAGAC 
AATCTGATTG TCCCTGGAGG TGTGAAGACC ATTGAGGCCA ATGGGAAGAT GGTGATGCCT 
GGAGGCATTG ATGTCCATAC CCACTTCCAG ATGCCTTACA AGGGGATGAC CACAGTGGAC 
GATTTCTTCC AAGGGACAAA GGCTGCCTTA GCGGGAGGAA CCACCATGAT CATTGACCAT 
GTGGTACCTG AACCTGAGTC TAGCCTGACC GAGGCCTATG AAAAGTGGCG TGAGTGGGCT 
GACGGGAAGA GCTGCTGTGA CTATGCTTTG CATGTGGACA TCACCCACTG GAATGACAGC 
GTCAAGCAAG AGGTGCAGAA CCTCAGTAAG GAAAAAGGCG TTAACTCCTT CATGGTTTAC 
ATGGCATACA AGGATTTATA TCAAGTGTCC AACACAGAGC TCTATGAGAT CTTCACCTGC 
CTGGGAGAAC TGGGGGCCAT TGCTCAAGTT CATGCCGAGA ATGGAGACAT CATTGCCCAG 
GAGCAGGCAC GAATGCTGGA AATGGGAATA ACAGGCCCAG AAGGTCATGT TCTGAGCAGA 
CCGGAAGAGC TGGAAGCTGA GGCTGTGTTC CGTGCCATCA CCGTCGCCAG CCAGACCAAC 
TGCCCCCTTT ATGTCACCAA GGTCATGAGC AAGAGCGCGG CTGATCTCAT CTCACAAGCC 
AGGAAGAAAG GAAATGTGGT CTTTGGCGAG CCCATCACTG CCAGCCTGGG AATAGATGGA 
ACCCATTACT GGAGTAAGAA CTGGGCCAAG GCAGCTGCAT TTGTGACATC CCCACCTCTG 
AGCCCTGACC CAACCACACC TGACTACATC AACTCCTTGC TGGCCAGTGG AGATCTGCAG 
CTCTCTGGAA GTGCCCACTG TACCTTCAGC ACTGCCCAGA AAGCCATTGG GAAGGACAAC 
TTCACGGCTA TCCCTGAGGG CACCAATGGC GTGGAGGAGC GTATGTCTGT CATCTGGGAC 
AAGGCTGTGG CCACAGGGAA GATGGATGAA AACCAGTTTG TGGCTGTGAC AAGTACCAAC 
GCTGCCAAGA TATTCAACCT GTACCCTCGC AAGGGGAGAA TAGCTGTGGG TTCTGACAGC 
GACCTTGTCA TCTGGGATCC AGATGCCGTG AAGATCGTCT CTGCCAAGAA CCACCAGTCG 
GTTGCGGAAT ACAACATCTT TGAAGGGATG GAGCTGCGTG GGGCACCTCT GGTGGTTATC 
TGCCAGGGCA AGATCATGCT GGAAGATGGT AACCTGCACG TGACCCAGGG GGCCGGCCGC 
TTCATTCCCT GCAGCCCATT CTCTGACTAT GTCTATAAGC GCATTAAAGC AAGGAGGAAG 
ATGGCGGACC TGCATGCAGT CCCAAGAGGC ATGTATGATG GACCAGTGTT TGACTTGACC 
ACCACCCCCA AGGGGGGCAC CCCAGCTGGC TCTACTCGAG GCTCTCCCAC TCGGCCAAAC 
CCACCAGTGA GGAACCTCCA TCAGTCGGGA TTTAGTCTGT CAGGCACCCA AGTGGATGAG 
GGTGTCCGCT CAGCCAGCAA ACGCATTGTG GCGCCCCCTG GAGGCCGTTC TAACATCACA 
TCCCTGAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons.
Brain research bulletin124286-94(2016 Jun)
Cha C,Zhang J,Ji Z,Tan M,Li S,Wu F,Chen K,Gong S,Guo G,Lin H

CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation.
Neural plasticity2015947423(2015)
Tan M,Cha C,Ye Y,Zhang J,Li S,Wu F,Gong S,Guo G

Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling.
Nature communications55325(2014 Oct)
Nakamura F,Kumeta K,Hida T,Isono T,Nakayama Y,Kuramata-Matsuoka E,Yamashita N,Uchida Y,Ogura K,Gengyo-Ando K,Mitani S,Ogino T,Goshima Y

Dihydropyrimidinase-like 3 regulates the inflammatory response of activated microglia.
Neuroscience25340-54(2013 Dec)
Manivannan J,Tay SS,Ling EA,Dheen ST

Injury-induced CRMP4 expression in adult sensory neurons; a possible target gene for ciliary neurotrophic factor.
Neuroscience letters485(1)37-42(2010 Nov)
Jang SY,Shin YK,Jung J,Lee SH,Seo SY,Suh DJ,Park HT