Sftpd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sftpd
Entrez Gene ID	25350
Full Name	surfactant protein D
Synonyms	SP-D,SPD
General protein information	Preferred Names surfactant protein D Names pulmonary surfactant-associated protein D CP4 PSP-D lung surfactant protein D pulmonary surfactant protein D surfactant associated protein D surfactant, pulmonary-associated protein D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	binds carbohydrates; may play a role in defense response in the lung [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012878.2	NP_037010.1	pulmonary surfactant-associated protein D precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SFTPD	NP_003010.4
Bos taurus (cattle)	SFTPD	NP_851369.1
Rattus norvegicus (Norway rat)	Sftpd	NP_037010.1
Canis lupus familiaris (dog)	SFTPD	XP_546184.2
Bos taurus (cattle)	CGN1	NP_783630.2
Mus musculus (house mouse)	Sftpd	NP_033186.1
Xenopus tropicalis (tropical clawed frog)	LOC100135366	NP_001107510.1
Pan troglodytes (chimpanzee)	SFTPD	XP_003312701.1
	SFTPD	NP_001035283.1
Bos taurus (cattle)	CL46	NP_001001856.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.
Rynkiewicz MJ, Wu H, Cafarella TR, Nikolaidis NM, Head JF, Seaton BA, McCormack FX
Biochemistry56(31)4095-4105(2017 08)

Surfactant Protein A Inhibits Growth and Adherence of Uropathogenic
Hashimoto J, Takahashi M, Saito A, Murata M, Kurimura Y, Nishitani C, Takamiya R, Uehara Y, Hasegawa Y, Hiyama Y, Sawada N, Takahashi S, Masumori N, Kuroki Y, Ariki S
Journal of immunology (Baltimore, Md. : 1950)198(7)2898-2905(2017 Apr)

Surfactant protein D modulates levels of IL-10 and TNF-? in intrauterine compartments during lipopolysaccharide-induced preterm birth.
Salminen A, Vuolteenaho R, Paananen R, Ojaniemi M, Hallman M
Cytokine60(2)423-30(2012 Nov)

Regulation of surfactant protein D expression by glucocorticoids in vitro and in vivo.
Deterding RR, Shimizu H, Fisher JH, Shannon JM
American journal of respiratory cell and molecular biology10(1)30-7(1994 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPA binds dipalmitoyl-phosphatidylcholine, the major surfactant lipid component, but not phosphatidylinositol; SPD exhibits the opposite preference. Data suggest flexibility in a key surface loop supports distinctive lipid binding of SPA; quadruple mutant SPA (E171D/P175E/R197N/K203D) that introduces SPD-like loop-stabilizing Ca2+ binding site in carbohydrate recognition domain exhibits ligand-binding preferences of SPD.
Title: Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.

in acute lung injury, serum level increased from day 5, peaked on day 10, and then gradually decreased until day 28
Title: Surfactant protein D is a useful biomarker for monitoring acute lung injury in rats.

In rat SP-D overexpressed mice, the lipopolysaccharide-induced levels of TNF-alpha and IL-10 in amniotic fluid and fetal serum and the expression of IL-10 in placenta and fetal membranes were significantly different from wild-type mice.
Title: Surfactant protein D modulates levels of IL-10 and TNF-? in intrauterine compartments during lipopolysaccharide-induced preterm birth.

Surfactant protein D modulated subpollen particle uptake in a cell type specific way (e.g. greater number of macrophages and epithelial cells, which participated in allergen particle uptake) and led to a decreased secretion of pro-inflammatory cytokines.
Title: Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model.

An extended binding site for influenza A virus; calcium-dependent antiviral activity involves residues flanking the primary carbohydrate binding site as well as more remote residues displayed on the carbohydrate recognition domain surface.
Title: Monoclonal antibody-assisted structure-function analysis of the carbohydrate recognition domain of surfactant protein D.

The following Sftpd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sftpd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42816	NM_012878.2 Latest version!	Rattus norvegicus surfactant protein D (Sftpd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sftpd ( NM_012878.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037010.1 Rattus norvegicus surfactant protein D (Sftpd), mRNA.

Price & Availability↑

CloneID	ORa42816
Clone ID Related Accession (Same CDS sequence)	NM_012878.2
Accession Version	NM_012878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein D precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M81231.1. On Mar 17, 2011 this sequence version replaced NM_012878.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M81231.1, BC070507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCACT TTCTCTCCAT GCTTGTCCTG CTTGTACAGC CCCTGGGCGA TCTGGGAGCA 
GAAATGAAGA CCCTTTCGCA GAGATCAATA ACCAACACGT GCACCCTAGT CTTGTGTAGT 
CCAACAGAGA ATGGCCTGCC TGGTCGTGAC GGACGGGATG GCAGAGAGGG TCCACGGGGC 
GAGAAGGGTG ATCCAGGTTT GCCAGGACCT ATGGGACTCT CAGGGTTGCC TGGCCCTAGA 
GGTCCAGTTG GGCCCAAAGG AGAGAATGGC TCTGCTGGAG AACCTGGACC AAAGGGAGAA 
CGTGGACTAG TTGGACCTCC AGGATCTCCA GGTATTTCTG GTCCGGCTGG AAAAGAAGGC 
CCCTCTGGGA AGCAGGGAAA CATAGGACCC CAAGGCAAAC CAGGTCCTAA AGGAGAGGCT 
GGGCCGAAAG GAGAAGTAGG TGCTCCCGGC ATGCAAGGAT CTGCAGGGGC AAAAGGCCCT 
GCTGGCCCCA AAGGAGAAAG AGGTGCCCCT GGTGAGCAAG GAGCCCCTGG GAATGCTGGG 
GCAGCAGGAC CTGCCGGACC TGCGGGTCCA CAGGGAGCTC CAGGTTCCAG GGGGCCTCCA 
GGACTCAAGG GGGACAGAGG TGCTCCTGGA GACAGAGGAA TCAAAGGCGA AAGTGGACTT 
CCAGACAGTG CTGCTCTGAG GCAGCAGATG GAGGCTTTGA ATGGAAAACT TCAGCGTCTA 
GAGGCTGCCT TTTCTCGCTA TAAGAAAGCC GCGCTCTTCC CTGATGGCCA AAGTGTTGGA 
GACAAAATCT TCAGGGCGGC AAACTCGGAA GAGCCTTTTG AGGATGCCAA GGAGATGTGC 
AGGCAGGCCG GAGGGCAGCT GGCCTCCCCA CGTTCTGCTA CTGAGAATGC TGCAGTACAG 
CAGCTCGTCA CAGCCCACAG CAAAGCTGCT TTCCTGAGTA TGACAGATGT GGGCACGGAG 
GGCAAGTTCA CTTACCCCAC AGGAGAGGCC CTGGTCTATT CTAACTGGGC TCCAGGGGAG 
CCCAACAACA ATGGAGGGGC AGAGAACTGT GTGGAGATCT TCACCAATGG GCAGTGGAAT 
GACAAGGCTT GTGGAGAGCA GCGCCTGGTT ATCTGTGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037010.1
CDS	19..1143
Translation	MLHFLSMLVLLVQPLGDLGAEMKTLSQRSITNTCTLVLCSPTENGLPGRDGRDGREGPRGEKGDPGLPGPMGLSGLPGPRGPVGPKGENGSAGEPGPKGERGLVGPPGSPGISGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGAKGPAGPKGERGAPGEQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGAPGDRGIKGESGLPDSAALRQQMEALNGKLQRLEAAFSRYKKAALFPDGQSVGDKIFRAANSEEPFEDAKEMCRQAGGQLASPRSATENAAVQQLVTAHSKAAFLSMTDVGTEGKFTYPTGEALVYSNWAPGEPNNNGGAENCVEIFTNGQWNDKACGEQRLVICEF

Target ORF information:

RefSeq Version	NM_012878.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein D (Sftpd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012878.2

ORF Insert Sequence:

ATGCTTCACT TTCTCTCCAT GCTTGTCCTG CTTGTACAGC CCCTGGGCGA TCTGGGAGCA 
GAAATGAAGA CCCTTTCGCA GAGATCAATA ACCAACACGT GCACCCTAGT CTTGTGTAGT 
CCAACAGAGA ATGGCCTGCC TGGTCGTGAC GGACGGGATG GCAGAGAGGG TCCACGGGGC 
GAGAAGGGTG ATCCAGGTTT GCCAGGACCT ATGGGACTCT CAGGGTTGCC TGGCCCTAGA 
GGTCCAGTTG GGCCCAAAGG AGAGAATGGC TCTGCTGGAG AACCTGGACC AAAGGGAGAA 
CGTGGACTAG TTGGACCTCC AGGATCTCCA GGTATTTCTG GTCCGGCTGG AAAAGAAGGC 
CCCTCTGGGA AGCAGGGAAA CATAGGACCC CAAGGCAAAC CAGGTCCTAA AGGAGAGGCT 
GGGCCGAAAG GAGAAGTAGG TGCTCCCGGC ATGCAAGGAT CTGCAGGGGC AAAAGGCCCT 
GCTGGCCCCA AAGGAGAAAG AGGTGCCCCT GGTGAGCAAG GAGCCCCTGG GAATGCTGGG 
GCAGCAGGAC CTGCCGGACC TGCGGGTCCA CAGGGAGCTC CAGGTTCCAG GGGGCCTCCA 
GGACTCAAGG GGGACAGAGG TGCTCCTGGA GACAGAGGAA TCAAAGGCGA AAGTGGACTT 
CCAGACAGTG CTGCTCTGAG GCAGCAGATG GAGGCTTTGA ATGGAAAACT TCAGCGTCTA 
GAGGCTGCCT TTTCTCGCTA TAAGAAAGCC GCGCTCTTCC CTGATGGCCA AAGTGTTGGA 
GACAAAATCT TCAGGGCGGC AAACTCGGAA GAGCCTTTTG AGGATGCCAA GGAGATGTGC 
AGGCAGGCCG GAGGGCAGCT GGCCTCCCCA CGTTCTGCTA CTGAGAATGC TGCAGTACAG 
CAGCTCGTCA CAGCCCACAG CAAAGCTGCT TTCCTGAGTA TGACAGATGT GGGCACGGAG 
GGCAAGTTCA CTTACCCCAC AGGAGAGGCC CTGGTCTATT CTAACTGGGC TCCAGGGGAG 
CCCAACAACA ATGGAGGGGC AGAGAACTGT GTGGAGATCT TCACCAATGG GCAGTGGAAT 
GACAAGGCTT GTGGAGAGCA GCGCCTGGTT ATCTGTGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.
Biochemistry56(31)4095-4105(2017 08)
Rynkiewicz MJ,Wu H,Cafarella TR,Nikolaidis NM,Head JF,Seaton BA,McCormack FX

Surfactant Protein A Inhibits Growth and Adherence of Uropathogenic
Journal of immunology (Baltimore, Md. : 1950)198(7)2898-2905(2017 Apr)
Hashimoto J,Takahashi M,Saito A,Murata M,Kurimura Y,Nishitani C,Takamiya R,Uehara Y,Hasegawa Y,Hiyama Y,Sawada N,Takahashi S,Masumori N,Kuroki Y,Ariki S

Surfactant protein D modulates levels of IL-10 and TNF-? in intrauterine compartments during lipopolysaccharide-induced preterm birth.
Cytokine60(2)423-30(2012 Nov)
Salminen A,Vuolteenaho R,Paananen R,Ojaniemi M,Hallman M

Regulation of surfactant protein D expression by glucocorticoids in vitro and in vivo.
American journal of respiratory cell and molecular biology10(1)30-7(1994 Jan)
Deterding RR,Shimizu H,Fisher JH,Shannon JM