Bhlha15 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bhlha15
Entrez Gene ID	25334
Full Name	basic helix-loop-helix family, member a15
Synonyms	Bhlhb8,Mist1,bHlH
General protein information	Preferred Names basic helix-loop-helix family, member a15 Names class A basic helix-loop-helix protein 15 Basic helix-loop-helix transcription factor (bHlH) MIST-1 basic helix-loop-helix domain containing, class B, 8 class B basic helix-loop-helix protein 8 muscle, intestine and stomach expression 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	basic helix-loop-helix transcription factor; may regulate gene expression in embryonic and postnatal cell lineages [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768941.2	XP_008767163.1	class A basic helix-loop-helix protein 15 isoform X1
NM_012863.1	NP_036995.1	class A basic helix-loop-helix protein 15

Pathways from BioSystems

Homologies

Homo sapiens (human)	BHLHA15	NP_803238.1
Canis lupus familiaris (dog)	BHLHA15	XP_005621166.1
Rattus norvegicus (Norway rat)	Bhlha15	NP_036995.1
Pan troglodytes (chimpanzee)	BHLHA15	XP_527829.1
Bos taurus (cattle)	BHLHA15	XP_005225330.1
Mus musculus (house mouse)	Bhlha15	NP_034930.1

Gene Ontology
Bibliography

Related articles in PubMed

XBP1 controls diverse cell type- and condition-specific transcriptional regulatory networks.
Acosta-Alvear D, Zhou Y, Blais A, Tsikitis M, Lents NH, Arias C, Lennon CJ, Kluger Y, Dynlacht BD
Molecular cell27(1)53-66(2007 Jul)

The bHLH domain of Mistl is sufficient to activate gene transcription.
Tran T, Jia D, Sun Y, Konieczny SF
Gene expression13(4-5)241-53(2007)

Altered Glut-2 accumulation and beta-cell function in mice lacking the exocrine-specific transcription factor, Mist1.
Fazio EN, Everest M, Colman R, Wang R, Pin CL
The Journal of endocrinology187(3)407-18(2005 Dec)

Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice.
Luo X, Shin DM, Wang X, Konieczny SF, Muallem S
The Journal of biological chemistry280(13)12668-75(2005 Apr)

Role of basic helix-loop-helix (bHLH) and CREB transcription factors in the regulation of Sertoli cell androgen-binding protein expression.
Saxlund MA, Sadler-Riggleman I, Skinner MK
Molecular reproduction and development68(3)269-78(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Mist1 activates exocrine-specific gene transcription through an acetylation mechanism.
Title: The bHLH domain of Mistl is sufficient to activate gene transcription.

bHLH regulate ABP in differentiated Sertoli cells.
Title: Role of basic helix-loop-helix (bHLH) and CREB transcription factors in the regulation of Sertoli cell androgen-binding protein expression.

The following Bhlha15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bhlha15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01151	NM_012863.1 Latest version!	Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa34596	XM_008768941.2 Latest version!	Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bhlha15 ( NM_012863.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036995.1 Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), mRNA.

Price & Availability↑

CloneID	ORa01151
Clone ID Related Accession (Same CDS sequence)	NM_012863.1
Accession Version	NM_012863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	class A basic helix-loop-helix protein 15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U58279.1. On Dec 17, 2003 this sequence version replaced XM_346425.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U58279.1, BC061868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACCA AGAACCGGCC CCCTCGGCGC CGGACACCCA TGCAGGACGC AGAAGCCACC 
CCAGGAGAGC AGACGCCTGA CAGATCCCAG TCAGGCTCAG GGGCATCAGA AGTGACCAAG 
GGTCTTCGGA GCAGGACAGC CCGTGCGAGC GGAACTCGGG CAGAGGTCAG CCGCCGGCGA 
CAGGGGTCCA GCAGCCGCAG GGAGAACAGC GTTCAGAGGC GGCTGGAGAG CAATGAGCGA 
GAAAGACAAC GGATGCACAA ACTGAACAAT GCTTTCCAGG CGCTGCGGGA GGTCATCCCG 
CATGTGCGGG CTGACAAGAA GCTCTCCAAG ATCGAGACCC TCACACTGGC CAAGAACTAT 
ATCAAGTCGC TGACAGCCAC CATACTTACT ATGTCCAGCA GCCGCCTCCC GGGGCTGGAG 
GCGCCAGGTC CTGCGCCAGG CCCTAAATTG TACCAGCATT ACCATCACCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGGT GGCTGGGGCC GTGCTCGGTG TCACCGAGGA CCAGCCTCAA 
GGCCACCTGC AACGATACTC TACACAGATC CACAGCTTCA GAGAGGGGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036995.1
CDS	125..718
Translation	MKTKNRPPRRRTPMQDAEATPGEQTPDRSQSGSGASEVTKGLRSRTARASGTRAEVSRRRQGSSSRRENSVQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEAPGPAPGPKLYQHYHHQQQQQQQQQQVAGAVLGVTEDQPQGHLQRYSTQIHSFREGS

Target ORF information:

RefSeq Version	NM_012863.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012863.1

ORF Insert Sequence:

ATGAAGACCA AGAACCGGCC CCCTCGGCGC CGGACACCCA TGCAGGACGC AGAAGCCACC 
CCAGGAGAGC AGACGCCTGA CAGATCCCAG TCAGGCTCAG GGGCATCAGA AGTGACCAAG 
GGTCTTCGGA GCAGGACAGC CCGTGCGAGC GGAACTCGGG CAGAGGTCAG CCGCCGGCGA 
CAGGGGTCCA GCAGCCGCAG GGAGAACAGC GTTCAGAGGC GGCTGGAGAG CAATGAGCGA 
GAAAGACAAC GGATGCACAA ACTGAACAAT GCTTTCCAGG CGCTGCGGGA GGTCATCCCG 
CATGTGCGGG CTGACAAGAA GCTCTCCAAG ATCGAGACCC TCACACTGGC CAAGAACTAT 
ATCAAGTCGC TGACAGCCAC CATACTTACT ATGTCCAGCA GCCGCCTCCC GGGGCTGGAG 
GCGCCAGGTC CTGCGCCAGG CCCTAAATTG TACCAGCATT ACCATCACCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGGT GGCTGGGGCC GTGCTCGGTG TCACCGAGGA CCAGCCTCAA 
GGCCACCTGC AACGATACTC TACACAGATC CACAGCTTCA GAGAGGGGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bhlha15 ( XM_008768941.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767163.1 Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34596
Clone ID Related Accession (Same CDS sequence)	XM_008768941.2
Accession Version	XM_008768941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	class A basic helix-loop-helix protein 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCCG CAGCCCCGCC TGTGGTGGCT AAAGCGACGT GTCCTTGTCC CCCGTGGGCA 
GCCTCTGCGC GTACGGCCTC GGATCCCCAG TCGGAAGGGC CTCTCCCAGC CACCACCTCC 
TGGGAAAAAC AGCCTAGAGC TATGAAGACC AAGAACCGGC CCCCTCGGCG CCGGACACCC 
ATGCAGGACG CAGAAGCCAC CCCAGGAGAG CAGACGCCTG ACAGATCCCA GTCAGGCTCA 
GGGGCATCAG AAGTGACCAA GGGTCTTCGG AGCAGGACAG CCCGTGCGAG CGGAACTCGG 
GCAGAGGTCA GCCGCCGGCG ACAGGGGTCC AGCAGCCGCA GGGAGAACAG CGTTCAGAGG 
CGGCTGGAGA GCAATGAGCG AGAAAGACAA CGGATGCACA AACTGAACAA TGCTTTCCAG 
GCGCTGCGGG AGGTCATCCC GCATGTGCGG GCTGACAAGA AGCTCTCCAA GATCGAGACC 
CTCACACTGG CCAAGAACTA TATCAAGTCG CTGACAGCCA CCATACTTAC TATGTCCAGC 
AGCCGCCTCC CGGGGCTGGA GGCGCCAGGT CCTGCGCCAG GCCCTAAATT GTACCAGCAT 
TACCATCACC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGG TGGCTGGGGC CGTGCTCGGT 
GTCACCGAGG ACCAGCCTCA AGGCCACCTG CAACGATACT CTACACAGAT CCACAGCTTC 
AGAGAGGGGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767163.1
CDS	1526..2260
Translation	MLPAAPPVVAKATCPCPPWAASARTASDPQSEGPLPATTSWEKQPRAMKTKNRPPRRRTPMQDAEATPGEQTPDRSQSGSGASEVTKGLRSRTARASGTRAEVSRRRQGSSSRRENSVQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEAPGPAPGPKLYQHYHHQQQQQQQQQQVAGAVLGVTEDQPQGHLQRYSTQIHSFREGS

Target ORF information:

RefSeq Version	XM_008768941.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basic helix-loop-helix family, member a15 (Bhlha15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768941.2

ORF Insert Sequence:

ATGCTTCCCG CAGCCCCGCC TGTGGTGGCT AAAGCGACGT GTCCTTGTCC CCCGTGGGCA 
GCCTCTGCGC GTACGGCCTC GGATCCCCAG TCGGAAGGGC CTCTCCCAGC CACCACCTCC 
TGGGAAAAAC AGCCTAGAGC TATGAAGACC AAGAACCGGC CCCCTCGGCG CCGGACACCC 
ATGCAGGACG CAGAAGCCAC CCCAGGAGAG CAGACGCCTG ACAGATCCCA GTCAGGCTCA 
GGGGCATCAG AAGTGACCAA GGGTCTTCGG AGCAGGACAG CCCGTGCGAG CGGAACTCGG 
GCAGAGGTCA GCCGCCGGCG ACAGGGGTCC AGCAGCCGCA GGGAGAACAG CGTTCAGAGG 
CGGCTGGAGA GCAATGAGCG AGAAAGACAA CGGATGCACA AACTGAACAA TGCTTTCCAG 
GCGCTGCGGG AGGTCATCCC GCATGTGCGG GCTGACAAGA AGCTCTCCAA GATCGAGACC 
CTCACACTGG CCAAGAACTA TATCAAGTCG CTGACAGCCA CCATACTTAC TATGTCCAGC 
AGCCGCCTCC CGGGGCTGGA GGCGCCAGGT CCTGCGCCAG GCCCTAAATT GTACCAGCAT 
TACCATCACC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGG TGGCTGGGGC CGTGCTCGGT 
GTCACCGAGG ACCAGCCTCA AGGCCACCTG CAACGATACT CTACACAGAT CCACAGCTTC 
AGAGAGGGGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

XBP1 controls diverse cell type- and condition-specific transcriptional regulatory networks.
Molecular cell27(1)53-66(2007 Jul)
Acosta-Alvear D,Zhou Y,Blais A,Tsikitis M,Lents NH,Arias C,Lennon CJ,Kluger Y,Dynlacht BD

The bHLH domain of Mistl is sufficient to activate gene transcription.
Gene expression13(4-5)241-53(2007)
Tran T,Jia D,Sun Y,Konieczny SF

Altered Glut-2 accumulation and beta-cell function in mice lacking the exocrine-specific transcription factor, Mist1.
The Journal of endocrinology187(3)407-18(2005 Dec)
Fazio EN,Everest M,Colman R,Wang R,Pin CL

Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice.
The Journal of biological chemistry280(13)12668-75(2005 Apr)
Luo X,Shin DM,Wang X,Konieczny SF,Muallem S

Role of basic helix-loop-helix (bHLH) and CREB transcription factors in the regulation of Sertoli cell androgen-binding protein expression.
Molecular reproduction and development68(3)269-78(2004 Jul)
Saxlund MA,Sadler-Riggleman I,Skinner MK