Gucy1b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gucy1b2
Entrez Gene ID	25206
Full Name	guanylate cyclase 1 soluble subunit beta 2
Synonyms	Gucy1b2a,Gucy1b2b,SGC
General protein information	Preferred Names guanylate cyclase 1 soluble subunit beta 2 Names guanylate cyclase soluble subunit beta-2 GCS-beta-2 Guanylate cyclase, soluble, beta 2 (GTP pyrophosphate - lyase) guanylate cyclase 1, soluble, beta 2 soluble guanylate cyclase beta 2a soluble guanylate cyclase beta 2b
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	subunit of soluble guanylate cyclase, which catalyzes the conversion of GTP to cyclic GMP; may be a candidate gene for involvement in blood pressure regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599584.1	XP_017455073.1	guanylate cyclase soluble subunit beta-2 isoform X3
NM_012770.2	NP_036902.2	guanylate cyclase soluble subunit beta-2 isoform 2
XM_008770741.2	XP_008768963.1	guanylate cyclase soluble subunit beta-2 isoform X4
XM_008770740.2	XP_008768962.1	guanylate cyclase soluble subunit beta-2 isoform X3
XM_008770737.2	XP_008768959.1	guanylate cyclase soluble subunit beta-2 isoform X1
XM_008770738.2	XP_008768960.1	guanylate cyclase soluble subunit beta-2 isoform X2
XM_008770739.2	XP_008768961.1	guanylate cyclase soluble subunit beta-2 isoform X3
NM_001270711.1	NP_001257640.1	guanylate cyclase soluble subunit beta-2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gucy1b2	NP_001257640.1
Xenopus tropicalis (tropical clawed frog)	LOC100496697	XP_002937143.2
Canis lupus familiaris (dog)	LOC485435	XP_005634132.1
Danio rerio (zebrafish)	LOC557191	XP_685297.5
Pan troglodytes (chimpanzee)	GUCY1B2	XP_509785.2
Mus musculus (house mouse)	Gucy1b2	NP_766398.1
Gallus gallus (chicken)	GUCY1B2	XP_426684.4

Gene Ontology
Bibliography

Related articles in PubMed

Dissociation of nitric oxide from soluble guanylate cyclase and heme-nitric oxide/oxygen binding domain constructs.
Winger JA, Derbyshire ER, Marletta MA
The Journal of biological chemistry282(2)897-907(2007 Jan)

Characterization of functional heme domains from soluble guanylate cyclase.
Karow DS, Pan D, Davis JH, Behrends S, Mathies RA, Marletta MA
Biochemistry44(49)16266-74(2005 Dec)

Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii.
Lin LH, Talman WT
Journal of chemical neuroanatomy29(2)127-36(2005 Mar)

Molecular cloning of a novel variant of the rat soluble guanylate cyclase beta2 subunit.
Okamoto H
The international journal of biochemistry & cell biology36(3)472-80(2004 Mar)

The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.
Behrends S, Budaeus L, Kempfert J, Scholz H, Starbatty J, Vehse K
Naunyn-Schmiedeberg's archives of pharmacology364(6)573-6(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

for the function of sGC in vivo, where there is evidence for two nitric oxide-regulated activity states
Title: Dissociation of nitric oxide from soluble guanylate cyclase and heme-nitric oxide/oxygen binding domain constructs.

Results identify and characterize the minimum functional ligand-binding heme domain derived from soluble guanylate cyclase.
Title: Characterization of functional heme domains from soluble guanylate cyclase.

These data support our hypothesis and provide anatomical support for the suggestion that nitroxidergic activation of the NTS involves sGC.
Title: Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii.

Expression of the beta 2 subunit of soluble guanylyl cyclase is switched on at a time when the urinary concentrating capacity develops, between postnatal days 16 to 22, increasing strongly in intensity in adult animals.
Title: The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.

The following Gucy1b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gucy1b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00890	NM_012770.2 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa09692	NM_001270711.1 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa00890	XM_008770740.2 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa00890	XM_008770739.2 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa00890	XM_017599584.1 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43025	XM_008770738.2 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43025	XM_008770737.2 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43026	XM_008770741.2 Latest version!	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gucy1b2 ( NM_012770.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036902.2 Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa00890
Clone ID Related Accession (Same CDS sequence)	XM_017599584.1 , NM_012770.2 , XM_008770740.2 , XM_008770739.2
Accession Version	NM_012770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M57507.1 and AB109451.1. On Jul 27, 2012 this sequence version replaced NM_012770.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: M57507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357 [ECO:0000348] ##Evidence-Data-END##

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036902.2
CDS	72..2195
Translation	MTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	NM_012770.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012770.2

ORF Insert Sequence:

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( NM_001270711.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257640.1 Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa09692
Clone ID Related Accession (Same CDS sequence)	NM_001270711.1
Accession Version	NM_001270711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY004153.2, M57507.1 and AB109451.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY004153.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTATGGAT TCATCAACAC CTGCCTGCAG TCTCTTGTGA CAGAGAAATT TGGTGAGGAG 
ACATGGGAGA AGCTGAAGGC TTCTGCAGAA GTGCAAGATG TCTTCATGAC CTACACCGTG 
TATGATGACA TCATCACCAT TAAGCTCATC CAAGAAGCCT GCAAGGTTCT GGATGTGTCC 
ATGGAAGCCA TTCTGAAGCT CTTTGGCGAA TACTTCTTTA AGTTCTGTAA GATGTCTGGC 
TATGACAGGA TGCTGCGGAC ACTTGGAGGA AATCTCACCG AGTTTATTGA AAACCTAGAT 
GCACTCCACA GTTACCTGGC ACTGTCCTAT CAGGAAATGA ACGCACCATC CTTTCGAGTG 
GAGGAAGGAG CTGACGGGGC GATGCTTCTC CACTACTACT CAGACAGACA TGGTCTGTGT 
CACATTGTAC CAGGTATCAT TGAAGCTGTG GCCAAGGACT TCTTTGACAC TGATGTGGCC 
ATGAGTATCC TGGATATGAA CGAAGAGGTG GAAAGGACAG GGAAGAAAGA ACATGTTGTG 
TTTCTGGTCG TGCAGAAGGC TCACAGACAG ATAAGAGGAG CAAAGGCAAG CCGGCCACAA 
GGCAGTGAGG ACAGCCAGGC AGACCAGGAG GCTCTCCAGG GAACACTCCT TCGGATGAAG 
GAGAGATATT TAAACATCCC TGTTTGCCCT GGGGAGAAAT CTCACTCAAC TGCTGTGAGG 
GCATCGGTCC TTTTTGGAAA AGGGCCCCTC AGGGACACCT TCCAGCCCGT CTATCCTGAG 
AGACTATGGG TCGAAGAGGA GGTGTTCTGT GATGCTTTTC CTTTCCACAT TGTCTTTGAT 
GAAGCACTAA GGGTCAAGCA AGCTGGAGTG AATATTCAGA AGTATGTCCC TGGAATCTTA 
ACCCAGAAGT TTGCACTAGA TGAGTATTTT TCCATCATCC ACCCTCAAGT TACTTTCAAC 
ATCTCCAGCA TCTGCAAGTT CATTAACAGT CAGTTTGTCT TGAAGACAAG AAAAGAAATG 
ATGCCCAAAG CAAGGAAGAG CCAGCCGATG CTCAAACTCC GGGGTCAGAT GATCTGGATG 
GAGTCTCTGA GGTGCATGAT CTTCATGTGT TCCCCAAAGC TCCGCAGCCT GCAAGAGCTG 
GAAGAGAGCA AGATGCATCT TTCTGATATC GCTCCGCACG ACACGACCAG GGATCTCATC 
CTCCTCAACC AGCAGAGGCT GGCAGAGATG GAGCTGTCCT GCCAACTGGA AAAGAAGAAG 
GAGGAGTTGC GTGTCCTTTC CAATCACCTG GCCATCGAGA AGAAGAAGAC AGAGACCTTG 
CTGTATGCCA TGCTGCCTGA ACATGTGGCC AACCAACTCA AGGAGGGCAG AAAGGTGGCT 
GCAGGAGAAT TTGAAACATG TACAATCCTT TTCAGCGATG TTGTGACATT TACCAACATC 
TGTGCAGCCT GTGAACCTAT CCAAATCGTG AACATGCTGA ATTCAATGTA CTCCAAGTTT 
GACAGGTTAA CCAGTGTCCA TGATGTCTAC AAAGTAGAAA CAATAGGGGA TGCTTACATG 
GTGGTGGGTG GAGTACCAGT ACCCGTTGAA AGCCATGCTC AAAGAGTCGC CAATTTTGCT 
CTGGGGATGA GAATTTCTGC AAAAGAAGTG ATGAATCCTG TCACTGGGGA ACCTATCCAG 
ATCAGAGTGG GAATCCACAC TGGACCAGTC TTAGCAGGTG TTGTGGGAGA CAAGATGCCT 
CGGTACTGCT TGTTTGGTGA CACTGTAAAC ACAGCCTCTA GGATGGAAAG TCACGGGCTT 
CCCAGCAAAG TGCATCTGAG CCCCACAGCC CACAGAGCCC TGAAAAACAA AGGGTTTGAA 
ATTGTCAGGA GAGGCGAGAT CGAAGTGAAG GGGAAAGGAA AGATGACCAC ATACTTTCTG 
ATCCAGAACC TGAATGCCAC CGAGGATGAG ATAATGGGGC GACCTTCAGC CCCCGCTGAT 
GGGAAGGAAG TATGTACTCC CGGAAACCAA GTCAGGAAGT CCCCTGCTGT CCCGAGGAAC 
ACAGACCATC AGCAACAAGT CTACAAAGGA GACCCAGCAG ACGCTTCTAA TGAAGTCACA 
CTTGCTGGGA GCCCAGTGGC AGGGCGAAAC TCCACAGATG CAGTCAATAA CCAGCCATCA 
CCAGATGAGA CCAAGACAAG TGTCGTTGCT AGTGGCCCTG TGCTGTCTGC TTTCTGTGTT 
GTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257640.1
CDS	84..2312
Translation	MYGFINTCLQSLVTEKFGEETWEKLKASAEVQDVFMTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	NM_001270711.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit beta 2 (Gucy1b2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270711.1

ORF Insert Sequence:

ATGTATGGAT TCATCAACAC CTGCCTGCAG TCTCTTGTGA CAGAGAAATT TGGTGAGGAG 
ACATGGGAGA AGCTGAAGGC TTCTGCAGAA GTGCAAGATG TCTTCATGAC CTACACCGTG 
TATGATGACA TCATCACCAT TAAGCTCATC CAAGAAGCCT GCAAGGTTCT GGATGTGTCC 
ATGGAAGCCA TTCTGAAGCT CTTTGGCGAA TACTTCTTTA AGTTCTGTAA GATGTCTGGC 
TATGACAGGA TGCTGCGGAC ACTTGGAGGA AATCTCACCG AGTTTATTGA AAACCTAGAT 
GCACTCCACA GTTACCTGGC ACTGTCCTAT CAGGAAATGA ACGCACCATC CTTTCGAGTG 
GAGGAAGGAG CTGACGGGGC GATGCTTCTC CACTACTACT CAGACAGACA TGGTCTGTGT 
CACATTGTAC CAGGTATCAT TGAAGCTGTG GCCAAGGACT TCTTTGACAC TGATGTGGCC 
ATGAGTATCC TGGATATGAA CGAAGAGGTG GAAAGGACAG GGAAGAAAGA ACATGTTGTG 
TTTCTGGTCG TGCAGAAGGC TCACAGACAG ATAAGAGGAG CAAAGGCAAG CCGGCCACAA 
GGCAGTGAGG ACAGCCAGGC AGACCAGGAG GCTCTCCAGG GAACACTCCT TCGGATGAAG 
GAGAGATATT TAAACATCCC TGTTTGCCCT GGGGAGAAAT CTCACTCAAC TGCTGTGAGG 
GCATCGGTCC TTTTTGGAAA AGGGCCCCTC AGGGACACCT TCCAGCCCGT CTATCCTGAG 
AGACTATGGG TCGAAGAGGA GGTGTTCTGT GATGCTTTTC CTTTCCACAT TGTCTTTGAT 
GAAGCACTAA GGGTCAAGCA AGCTGGAGTG AATATTCAGA AGTATGTCCC TGGAATCTTA 
ACCCAGAAGT TTGCACTAGA TGAGTATTTT TCCATCATCC ACCCTCAAGT TACTTTCAAC 
ATCTCCAGCA TCTGCAAGTT CATTAACAGT CAGTTTGTCT TGAAGACAAG AAAAGAAATG 
ATGCCCAAAG CAAGGAAGAG CCAGCCGATG CTCAAACTCC GGGGTCAGAT GATCTGGATG 
GAGTCTCTGA GGTGCATGAT CTTCATGTGT TCCCCAAAGC TCCGCAGCCT GCAAGAGCTG 
GAAGAGAGCA AGATGCATCT TTCTGATATC GCTCCGCACG ACACGACCAG GGATCTCATC 
CTCCTCAACC AGCAGAGGCT GGCAGAGATG GAGCTGTCCT GCCAACTGGA AAAGAAGAAG 
GAGGAGTTGC GTGTCCTTTC CAATCACCTG GCCATCGAGA AGAAGAAGAC AGAGACCTTG 
CTGTATGCCA TGCTGCCTGA ACATGTGGCC AACCAACTCA AGGAGGGCAG AAAGGTGGCT 
GCAGGAGAAT TTGAAACATG TACAATCCTT TTCAGCGATG TTGTGACATT TACCAACATC 
TGTGCAGCCT GTGAACCTAT CCAAATCGTG AACATGCTGA ATTCAATGTA CTCCAAGTTT 
GACAGGTTAA CCAGTGTCCA TGATGTCTAC AAAGTAGAAA CAATAGGGGA TGCTTACATG 
GTGGTGGGTG GAGTACCAGT ACCCGTTGAA AGCCATGCTC AAAGAGTCGC CAATTTTGCT 
CTGGGGATGA GAATTTCTGC AAAAGAAGTG ATGAATCCTG TCACTGGGGA ACCTATCCAG 
ATCAGAGTGG GAATCCACAC TGGACCAGTC TTAGCAGGTG TTGTGGGAGA CAAGATGCCT 
CGGTACTGCT TGTTTGGTGA CACTGTAAAC ACAGCCTCTA GGATGGAAAG TCACGGGCTT 
CCCAGCAAAG TGCATCTGAG CCCCACAGCC CACAGAGCCC TGAAAAACAA AGGGTTTGAA 
ATTGTCAGGA GAGGCGAGAT CGAAGTGAAG GGGAAAGGAA AGATGACCAC ATACTTTCTG 
ATCCAGAACC TGAATGCCAC CGAGGATGAG ATAATGGGGC GACCTTCAGC CCCCGCTGAT 
GGGAAGGAAG TATGTACTCC CGGAAACCAA GTCAGGAAGT CCCCTGCTGT CCCGAGGAAC 
ACAGACCATC AGCAACAAGT CTACAAAGGA GACCCAGCAG ACGCTTCTAA TGAAGTCACA 
CTTGCTGGGA GCCCAGTGGC AGGGCGAAAC TCCACAGATG CAGTCAATAA CCAGCCATCA 
CCAGATGAGA CCAAGACAAG TGTCGTTGCT AGTGGCCCTG TGCTGTCTGC TTTCTGTGTT 
GTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_017599584.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455073.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa00890
Clone ID Related Accession (Same CDS sequence)	XM_017599584.1 , NM_012770.2 , XM_008770740.2 , XM_008770739.2
Accession Version	XM_017599584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455073.1
CDS	122..2245
Translation	MTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	XM_017599584.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599584.1

ORF Insert Sequence:

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_008770740.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768962.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa00890
Clone ID Related Accession (Same CDS sequence)	XM_017599584.1 , NM_012770.2 , XM_008770740.2 , XM_008770739.2
Accession Version	XM_008770740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768962.1
CDS	452..2575
Translation	MTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	XM_008770740.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770740.2

ORF Insert Sequence:

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_008770739.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768961.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa00890
Clone ID Related Accession (Same CDS sequence)	XM_017599584.1 , NM_012770.2 , XM_008770740.2 , XM_008770739.2
Accession Version	XM_008770739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768961.1
CDS	2774..4897
Translation	MTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	XM_008770739.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770739.2

ORF Insert Sequence:

ATGACCTACA CCGTGTATGA TGACATCATC ACCATTAAGC TCATCCAAGA AGCCTGCAAG 
GTTCTGGATG TGTCCATGGA AGCCATTCTG AAGCTCTTTG GCGAATACTT CTTTAAGTTC 
TGTAAGATGT CTGGCTATGA CAGGATGCTG CGGACACTTG GAGGAAATCT CACCGAGTTT 
ATTGAAAACC TAGATGCACT CCACAGTTAC CTGGCACTGT CCTATCAGGA AATGAACGCA 
CCATCCTTTC GAGTGGAGGA AGGAGCTGAC GGGGCGATGC TTCTCCACTA CTACTCAGAC 
AGACATGGTC TGTGTCACAT TGTACCAGGT ATCATTGAAG CTGTGGCCAA GGACTTCTTT 
GACACTGATG TGGCCATGAG TATCCTGGAT ATGAACGAAG AGGTGGAAAG GACAGGGAAG 
AAAGAACATG TTGTGTTTCT GGTCGTGCAG AAGGCTCACA GACAGATAAG AGGAGCAAAG 
GCAAGCCGGC CACAAGGCAG TGAGGACAGC CAGGCAGACC AGGAGGCTCT CCAGGGAACA 
CTCCTTCGGA TGAAGGAGAG ATATTTAAAC ATCCCTGTTT GCCCTGGGGA GAAATCTCAC 
TCAACTGCTG TGAGGGCATC GGTCCTTTTT GGAAAAGGGC CCCTCAGGGA CACCTTCCAG 
CCCGTCTATC CTGAGAGACT ATGGGTCGAA GAGGAGGTGT TCTGTGATGC TTTTCCTTTC 
CACATTGTCT TTGATGAAGC ACTAAGGGTC AAGCAAGCTG GAGTGAATAT TCAGAAGTAT 
GTCCCTGGAA TCTTAACCCA GAAGTTTGCA CTAGATGAGT ATTTTTCCAT CATCCACCCT 
CAAGTTACTT TCAACATCTC CAGCATCTGC AAGTTCATTA ACAGTCAGTT TGTCTTGAAG 
ACAAGAAAAG AAATGATGCC CAAAGCAAGG AAGAGCCAGC CGATGCTCAA ACTCCGGGGT 
CAGATGATCT GGATGGAGTC TCTGAGGTGC ATGATCTTCA TGTGTTCCCC AAAGCTCCGC 
AGCCTGCAAG AGCTGGAAGA GAGCAAGATG CATCTTTCTG ATATCGCTCC GCACGACACG 
ACCAGGGATC TCATCCTCCT CAACCAGCAG AGGCTGGCAG AGATGGAGCT GTCCTGCCAA 
CTGGAAAAGA AGAAGGAGGA GTTGCGTGTC CTTTCCAATC ACCTGGCCAT CGAGAAGAAG 
AAGACAGAGA CCTTGCTGTA TGCCATGCTG CCTGAACATG TGGCCAACCA ACTCAAGGAG 
GGCAGAAAGG TGGCTGCAGG AGAATTTGAA ACATGTACAA TCCTTTTCAG CGATGTTGTG 
ACATTTACCA ACATCTGTGC AGCCTGTGAA CCTATCCAAA TCGTGAACAT GCTGAATTCA 
ATGTACTCCA AGTTTGACAG GTTAACCAGT GTCCATGATG TCTACAAAGT AGAAACAATA 
GGGGATGCTT ACATGGTGGT GGGTGGAGTA CCAGTACCCG TTGAAAGCCA TGCTCAAAGA 
GTCGCCAATT TTGCTCTGGG GATGAGAATT TCTGCAAAAG AAGTGATGAA TCCTGTCACT 
GGGGAACCTA TCCAGATCAG AGTGGGAATC CACACTGGAC CAGTCTTAGC AGGTGTTGTG 
GGAGACAAGA TGCCTCGGTA CTGCTTGTTT GGTGACACTG TAAACACAGC CTCTAGGATG 
GAAAGTCACG GGCTTCCCAG CAAAGTGCAT CTGAGCCCCA CAGCCCACAG AGCCCTGAAA 
AACAAAGGGT TTGAAATTGT CAGGAGAGGC GAGATCGAAG TGAAGGGGAA AGGAAAGATG 
ACCACATACT TTCTGATCCA GAACCTGAAT GCCACCGAGG ATGAGATAAT GGGGCGACCT 
TCAGCCCCCG CTGATGGGAA GGAAGTATGT ACTCCCGGAA ACCAAGTCAG GAAGTCCCCT 
GCTGTCCCGA GGAACACAGA CCATCAGCAA CAAGTCTACA AAGGAGACCC AGCAGACGCT 
TCTAATGAAG TCACACTTGC TGGGAGCCCA GTGGCAGGGC GAAACTCCAC AGATGCAGTC 
AATAACCAGC CATCACCAGA TGAGACCAAG ACAAGTGTCG TTGCTAGTGG CCCTGTGCTG 
TCTGCTTTCT GTGTTGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_008770737.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768959.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43025
Clone ID Related Accession (Same CDS sequence)	XM_008770737.2 , XM_008770738.2
Accession Version	XM_008770737.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG CTTCTAATGA AGTCACACTT GCTGGGAGCC CAGTGGCAGG GCGAAACTCC 
ACAGATGCAG TCAATAACCA GCCATCACCA GATGAGACCA AGACAAGTGT CGTTGCTAGT 
GGCCCTGTGC TGTCTGCTTT CTGTGTTGTG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768959.1
CDS	551..2806
Translation	MCLFTSLWNLYGFINTCLQSLVTEKFGEETWEKLKASAEVQDVFMTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	XM_008770737.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770737.2

ORF Insert Sequence:

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG CTTCTAATGA AGTCACACTT GCTGGGAGCC CAGTGGCAGG GCGAAACTCC 
ACAGATGCAG TCAATAACCA GCCATCACCA GATGAGACCA AGACAAGTGT CGTTGCTAGT 
GGCCCTGTGC TGTCTGCTTT CTGTGTTGTG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_008770738.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768960.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43025
Clone ID Related Accession (Same CDS sequence)	XM_008770737.2 , XM_008770738.2
Accession Version	XM_008770738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG CTTCTAATGA AGTCACACTT GCTGGGAGCC CAGTGGCAGG GCGAAACTCC 
ACAGATGCAG TCAATAACCA GCCATCACCA GATGAGACCA AGACAAGTGT CGTTGCTAGT 
GGCCCTGTGC TGTCTGCTTT CTGTGTTGTG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768960.1
CDS	551..2806
Translation	MCLFTSLWNLYGFINTCLQSLVTEKFGEETWEKLKASAEVQDVFMTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADASNEVTLAGSPVAGRNSTDAVNNQPSPDETKTSVVASGPVLSAFCVVL

Target ORF information:

RefSeq Version	XM_008770738.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770738.2

ORF Insert Sequence:

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG CTTCTAATGA AGTCACACTT GCTGGGAGCC CAGTGGCAGG GCGAAACTCC 
ACAGATGCAG TCAATAACCA GCCATCACCA GATGAGACCA AGACAAGTGT CGTTGCTAGT 
GGCCCTGTGC TGTCTGCTTT CTGTGTTGTG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1b2 ( XM_008770741.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768963.1 Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X6, mRNA.

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CloneID	ORa43026
Clone ID Related Accession (Same CDS sequence)	XM_008770741.2
Accession Version	XM_008770741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit beta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG GAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768963.1
CDS	551..2668
Translation	MCLFTSLWNLYGFINTCLQSLVTEKFGEETWEKLKASAEVQDVFMTYTVYDDIITIKLIQEACKVLDVSMEAILKLFGEYFFKFCKMSGYDRMLRTLGGNLTEFIENLDALHSYLALSYQEMNAPSFRVEEGADGAMLLHYYSDRHGLCHIVPGIIEAVAKDFFDTDVAMSILDMNEEVERTGKKEHVVFLVVQKAHRQIRGAKASRPQGSEDSQADQEALQGTLLRMKERYLNIPVCPGEKSHSTAVRASVLFGKGPLRDTFQPVYPERLWVEEEVFCDAFPFHIVFDEALRVKQAGVNIQKYVPGILTQKFALDEYFSIIHPQVTFNISSICKFINSQFVLKTRKEMMPKARKSQPMLKLRGQMIWMESLRCMIFMCSPKLRSLQELEESKMHLSDIAPHDTTRDLILLNQQRLAEMELSCQLEKKKEELRVLSNHLAIEKKKTETLLYAMLPEHVANQLKEGRKVAAGEFETCTILFSDVVTFTNICAACEPIQIVNMLNSMYSKFDRLTSVHDVYKVETIGDAYMVVGGVPVPVESHAQRVANFALGMRISAKEVMNPVTGEPIQIRVGIHTGPVLAGVVGDKMPRYCLFGDTVNTASRMESHGLPSKVHLSPTAHRALKNKGFEIVRRGEIEVKGKGKMTTYFLIQNLNATEDEIMGRPSAPADGKEVCTPGNQVRKSPAVPRNTDHQQQVYKGDPADGT

Target ORF information:

RefSeq Version	XM_008770741.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1, soluble, beta 2 (Gucy1b2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770741.2

ORF Insert Sequence:

ATGTGTCTTT TCACTTCTCT TTGGAACCTG TATGGATTCA TCAACACCTG CCTGCAGTCT 
CTTGTGACAG AGAAATTTGG TGAGGAGACA TGGGAGAAGC TGAAGGCTTC TGCAGAAGTG 
CAAGATGTCT TCATGACCTA CACCGTGTAT GATGACATCA TCACCATTAA GCTCATCCAA 
GAAGCCTGCA AGGTTCTGGA TGTGTCCATG GAAGCCATTC TGAAGCTCTT TGGCGAATAC 
TTCTTTAAGT TCTGTAAGAT GTCTGGCTAT GACAGGATGC TGCGGACACT TGGAGGAAAT 
CTCACCGAGT TTATTGAAAA CCTAGATGCA CTCCACAGTT ACCTGGCACT GTCCTATCAG 
GAAATGAACG CACCATCCTT TCGAGTGGAG GAAGGAGCTG ACGGGGCGAT GCTTCTCCAC 
TACTACTCAG ACAGACATGG TCTGTGTCAC ATTGTACCAG GTATCATTGA AGCTGTGGCC 
AAGGACTTCT TTGACACTGA TGTGGCCATG AGTATCCTGG ATATGAACGA AGAGGTGGAA 
AGGACAGGGA AGAAAGAACA TGTTGTGTTT CTGGTCGTGC AGAAGGCTCA CAGACAGATA 
AGAGGAGCAA AGGCAAGCCG GCCACAAGGC AGTGAGGACA GCCAGGCAGA CCAGGAGGCT 
CTCCAGGGAA CACTCCTTCG GATGAAGGAG AGATATTTAA ACATCCCTGT TTGCCCTGGG 
GAGAAATCTC ACTCAACTGC TGTGAGGGCA TCGGTCCTTT TTGGAAAAGG GCCCCTCAGG 
GACACCTTCC AGCCCGTCTA TCCTGAGAGA CTATGGGTCG AAGAGGAGGT GTTCTGTGAT 
GCTTTTCCTT TCCACATTGT CTTTGATGAA GCACTAAGGG TCAAGCAAGC TGGAGTGAAT 
ATTCAGAAGT ATGTCCCTGG AATCTTAACC CAGAAGTTTG CACTAGATGA GTATTTTTCC 
ATCATCCACC CTCAAGTTAC TTTCAACATC TCCAGCATCT GCAAGTTCAT TAACAGTCAG 
TTTGTCTTGA AGACAAGAAA AGAAATGATG CCCAAAGCAA GGAAGAGCCA GCCGATGCTC 
AAACTCCGGG GTCAGATGAT CTGGATGGAG TCTCTGAGGT GCATGATCTT CATGTGTTCC 
CCAAAGCTCC GCAGCCTGCA AGAGCTGGAA GAGAGCAAGA TGCATCTTTC TGATATCGCT 
CCGCACGACA CGACCAGGGA TCTCATCCTC CTCAACCAGC AGAGGCTGGC AGAGATGGAG 
CTGTCCTGCC AACTGGAAAA GAAGAAGGAG GAGTTGCGTG TCCTTTCCAA TCACCTGGCC 
ATCGAGAAGA AGAAGACAGA GACCTTGCTG TATGCCATGC TGCCTGAACA TGTGGCCAAC 
CAACTCAAGG AGGGCAGAAA GGTGGCTGCA GGAGAATTTG AAACATGTAC AATCCTTTTC 
AGCGATGTTG TGACATTTAC CAACATCTGT GCAGCCTGTG AACCTATCCA AATCGTGAAC 
ATGCTGAATT CAATGTACTC CAAGTTTGAC AGGTTAACCA GTGTCCATGA TGTCTACAAA 
GTAGAAACAA TAGGGGATGC TTACATGGTG GTGGGTGGAG TACCAGTACC CGTTGAAAGC 
CATGCTCAAA GAGTCGCCAA TTTTGCTCTG GGGATGAGAA TTTCTGCAAA AGAAGTGATG 
AATCCTGTCA CTGGGGAACC TATCCAGATC AGAGTGGGAA TCCACACTGG ACCAGTCTTA 
GCAGGTGTTG TGGGAGACAA GATGCCTCGG TACTGCTTGT TTGGTGACAC TGTAAACACA 
GCCTCTAGGA TGGAAAGTCA CGGGCTTCCC AGCAAAGTGC ATCTGAGCCC CACAGCCCAC 
AGAGCCCTGA AAAACAAAGG GTTTGAAATT GTCAGGAGAG GCGAGATCGA AGTGAAGGGG 
AAAGGAAAGA TGACCACATA CTTTCTGATC CAGAACCTGA ATGCCACCGA GGATGAGATA 
ATGGGGCGAC CTTCAGCCCC CGCTGATGGG AAGGAAGTAT GTACTCCCGG AAACCAAGTC 
AGGAAGTCCC CTGCTGTCCC GAGGAACACA GACCATCAGC AACAAGTCTA CAAAGGAGAC 
CCAGCAGACG GAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissociation of nitric oxide from soluble guanylate cyclase and heme-nitric oxide/oxygen binding domain constructs.
The Journal of biological chemistry282(2)897-907(2007 Jan)
Winger JA,Derbyshire ER,Marletta MA

Characterization of functional heme domains from soluble guanylate cyclase.
Biochemistry44(49)16266-74(2005 Dec)
Karow DS,Pan D,Davis JH,Behrends S,Mathies RA,Marletta MA

Soluble guanylate cyclase and neuronal nitric oxide synthase colocalize in rat nucleus tractus solitarii.
Journal of chemical neuroanatomy29(2)127-36(2005 Mar)
Lin LH,Talman WT

Molecular cloning of a novel variant of the rat soluble guanylate cyclase beta2 subunit.
The international journal of biochemistry & cell biology36(3)472-80(2004 Mar)
Okamoto H

The beta 2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney.
Naunyn-Schmiedeberg's archives of pharmacology364(6)573-6(2001 Dec)
Behrends S,Budaeus L,Kempfert J,Scholz H,Starbatty J,Vehse K

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Gucy1b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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