Stat5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stat5b
Entrez Gene ID	25126
Full Name	signal transducer and activator of transcription 5B
General protein information	Preferred Names signal transducer and activator of transcription 5B Names signal transducer and activator of transcription 5B Signal transducer and activator of transcription 5a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	member of the STAT family of transcription factors; may mediate biological actions of several interleukins and growth hormones; may be associated with mammary gland development and lactation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022380.1	NP_071775.1	signal transducer and activator of transcription 5B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	STAT5B	NP_777042.2
Gallus gallus (chicken)	STAT5B	NP_990110.1
	STAT5B	XP_001109317.2
Danio rerio (zebrafish)	stat5.2	NP_001003984.1
Homo sapiens (human)	STAT5B	NP_036580.2
Mus musculus (house mouse)	Stat5b	NP_035619.3
Caenorhabditis elegans (roundworm)	sta-1	NP_502974.2
Pan troglodytes (chimpanzee)	STAT5B	XP_001166251.1
Canis lupus familiaris (dog)	STAT5B	XP_548092.1
Rattus norvegicus (Norway rat)	Stat5b	NP_071775.1
Danio rerio (zebrafish)	stat5.1	NP_919368.1
Xenopus tropicalis (tropical clawed frog)	stat5b	XP_002943467.1

Gene Ontology
Bibliography

Related articles in PubMed

Genotype-dependent effects of TGF-?1 on mast cell function: targeting the Stat5 pathway.
Fernando J, Faber TW, Pullen NA, Falanga YT, Kolawole EM, Oskeritzian CA, Barnstein BO, Bandara G, Li G, Schwartz LB, Spiegel S, Straus DB, Conrad DH, Bunting KD, Ryan JJ
Journal of immunology (Baltimore, Md. : 1950)191(9)4505-13(2013 Nov)

Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3.
Fernandez DJ, Tuma DJ, Tuma PL
American journal of physiology. Gastrointestinal and liver physiology303(12)G1402-15(2012 Dec)

The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.
Krebs CJ, Schultz DC, Robins DM
Molecular and cellular biology32(18)3732-42(2012 Sep)

Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription.
Varco-Merth B, Mirza K, Alzhanov DT, Chia DJ, Rotwein P
PloS one7(11)e50278(2012)

MSM enhances GH signaling via the Jak2/STAT5b pathway in osteoblast-like cells and osteoblast differentiation through the activation of STAT5b in MSCs.
Joung YH, Lim EJ, Darvin P, Chung SC, Jang JW, Do Park K, Lee HK, Kim HS, Park T, Yang YM
PloS one7(10)e47477(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus the alcohol-induced impairment of STAT nuclear translocation can be explained by increased microtubule acetylation and stability.
Title: Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3.

Combinatorial interplay among multiple Stat5b-binding response elements with distinguishable biochemical properties is responsible for highly regulated control of IGF-I gene activity by growth hormone.
Title: Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription.

STAT5b plays an essential role in growth hormone signaling activation and osteoblast differentiation.
Title: MSM enhances GH signaling via the Jak2/STAT5b pathway in osteoblast-like cells and osteoblast differentiation through the activation of STAT5b in MSCs.

the inhibition of Stat5b expression in chondrocytes prevented the GH promoting effects on NF-kappaB-DNA binding, whereas the inhibition of NF-kappaB p65 expression or activity prevented the GH-dependent activation of IGF-1 and bmp2 expression.
Title: Nuclear factor-kappaB (NF-kappaB) p65 interacts with Stat5b in growth plate chondrocytes and mediates the effects of growth hormone on chondrogenesis and on the expression of insulin-like growth factor-1 and bone morphogenetic protein-2.

GH stimulates recruitment of Stat5b to multiple dispersed regions within the IGFI locus
Title: Dispersed Chromosomal Stat5b-binding elements mediate growth hormone-activated insulin-like growth factor-I gene transcription.

The following Stat5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stat5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05680	NM_022380.1 Latest version!	Rattus norvegicus signal transducer and activator of transcription 5B (Stat5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$461.30 $659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stat5b ( NM_022380.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071775.1 Rattus norvegicus signal transducer and activator of transcription 5B (Stat5b), mRNA.

Price & Availability↑

CloneID	ORa05680
Clone ID Related Accession (Same CDS sequence)	NM_022380.1
Accession Version	NM_022380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Rattus norvegicus(Norway rat)
Product	signal transducer and activator of transcription 5B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X97541.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X97541.1, X91988.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAATGT GGATACAGGC TCAGCAGCTC CAGGGCGATG CCCTTCATCA GATGCAGGCC 
TTGTACGGCC AGCATTTCCC CATCGAGGTG CGACATTATT TATCCCAGTG GATCGAAAGT 
CAAGCCTGGG ACTCAATAGA TCTTGATAAT CCACAGGAGA ACATTAAGGC CACCCAGCTC 
CTGGAGGGCC TGGTGCAGGA GCTGCAGAAG AAGGCAGAAC ACCAGGTGGG GGAAGATGGG 
TTTTTGCTGA AGATCAAGCT GGGGCACTAT GCCACGCAGC TCCAGAACAC GTATGACCGC 
TGCCCCATGG AGCTGGTTCG CTGTATCCGG CACATTCTAT ACAATGAACA GAGGCTGGTC 
CGAGAAGCCA ACAACGGAAG CTCTCCGGCT GGAAGTCTTG CTGACGCCAT GTCCCAGAAG 
CACCTTCAGA TCAACCAAAC GTTTGAGGAG CTGCGTCTGA TCACACAGGA CACGGAGAGC 
GAGCTGAAGA AGCTGCAGCA GACCCAAGAG TACTTCATCA TCCAGTACCA GGAGAGCCTA 
CGGATCCAAG CTCAGTTTGC CCAGCTGGCC CAACTGAACC CCCAGGAGCG CATGAGCAGG 
GAGACAGCCC TCCAGCAGAA GCAAGTGTCC CTGGAGACCT GGCTGCAGCG AGAGGCACAG 
ACACTGCAGC AGTACCGCGT GGAGCTGGCT GAAAAGCACC AGAAGACCCT GCAGCTGCTG 
AGGAAGCAGC AGACCATCAT CCTGGACGAC GAGCTGATCC AGTGGAAGCG GCGACAGCAA 
CTGGCCGGGA ATGGGGGTCC TCCTGAGGGC AGCCTGGATG TGCTGCAGTC CTGGTGTGAG 
AAGCTGGCCG AGATCATCTG GCAGAACCGG CAGCAGATCC GCAGGGCTGA GCACCTGTGC 
CAGCAGCTGC CCATCCCAGG CCCCGTGGAG GAGATGCTGG CCGAGGTCAA CGCCACCATC 
ACAGACATCA TCTCAGCCCT GGTCACCAGC ACGTTCATCA TCGAGAAGCA GCCTCCTCAG 
GTCCTGAAGA CCCAGACCAA GTTCGCGGCC ACCGTGCGCC TGCTGGTGGG CGGGAAGCTG 
AACGTGCACA TGAACCCTCC GCAGGTGAAG GCAACCATCA TCAGCGAGCA GCAGGCCAAG 
TCCCTGCTCA AGAACGAGAA CACCCGCAAT GATTATAGCG GCGAGATCCT GAACAACTGC 
TGCGTCATGG AGTACCACCA GGCCACAGGC ACCCTCAGCG CCCACTTCAG AAACATGTCC 
CTGAAACGAA TCAAGAGGTC TGACCGCCGT GGTGCAGAGT CGGTCACGGA AGAGAAGTTC 
ACAATCTTGT TTGACTCACA GTTCAGTGTT GGTGGAAATG AGCTGGTCTT TCAAGTCAAG 
ACCTTGTCGC TCCCGGTGGT GGTGATCGTT CACGGCAGCC AGGACAACAA TGCGACGGCT 
ACCGTCCTCT GGGACAACGC CTTTGCAGAG CCTGGCAGGG TGCCATTTGC CGTGCCTGAC 
AAGGTGCTGT GGCCGCAGCT GTGTGAGGCG CTCAACATGA AGTTCAAGGC TGAGGTGCAG 
AGCAACCGGG GCTTGACCAA GGAGAACCTC GTGTTCCTGG CACAGAAACT GTTCAACAGC 
AGCAGCAACC ACCTCGAGGA CTACAACAGC ATGTCCGTGT CCTGGTCCCA GTTCAACCGG 
GAGAATCTGC CAGGACGGAA TTACACTTTC TGGCAGTGGT TTGACGGTGT GATGGAGGTG 
TTAAAGAAAC ATCTCAAGCC TCACTGGAAC GATGGGGCCA TCTTGGGTTT CGTGAACAAG 
CAGCAGGCCC ACGACCTGCT CATCAACAAG CCAGATGGGA CCTTCCTGCT GCGCTTCAGC 
GACTCGGAAA TCGGGGGCAT CACCATTGCT TGGAAGTTTG ACTCCCAGGA GAGAATGTTT 
TGGAATCTGA TGCCTTTTAC CACAAGAGAC TTCTCTATCC GGTCCCTCGC TGACCGCCTG 
GGGGACCTGA ATTACCTCAT ATACGTGTTT CCTGATCGGC CAAAGGATGA AGTATATTCT 
AAGTACTACA CACCAGTCCC GTGTGAGCCC GCCACCGCGA AAGCAGCTGA CGGATACGTG 
AAGCCGCAGA TCAAGCAAGT GGTCCCCGAG TTTGTCAATG CTTCTGCAGA TGCAGGGAGC 
GGCGCCACCT ACATGGACCA GGCTCCTTCC CCAGTGGTGT GCCCTCAGGC TCACTATAAC 
ATGTACCCAC AGAATCCCGA CTCTGTCCTC GATACCGATG GGGATTTCGA TCTGGAAGAC 
ACGATGGACG TGGCACGGCG TGTGGAAGAG CTCTTAGGCC GGCCCATGGA CAGTCAGTGG 
ATCCCTCACG CGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071775.1
CDS	111..2471
Translation	MAMWIQAQQLQGDALHQMQALYGQHFPIEVRHYLSQWIESQAWDSIDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLITQDTESELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLNPQERMSRETALQQKQVSLETWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFDSQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNSSSNHLEDYNSMSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCEPATAKAADGYVKPQIKQVVPEFVNASADAGSGATYMDQAPSPVVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS

Target ORF information:

RefSeq Version	NM_022380.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal transducer and activator of transcription 5B (Stat5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022380.1

ORF Insert Sequence:

ATGGCAATGT GGATACAGGC TCAGCAGCTC CAGGGCGATG CCCTTCATCA GATGCAGGCC 
TTGTACGGCC AGCATTTCCC CATCGAGGTG CGACATTATT TATCCCAGTG GATCGAAAGT 
CAAGCCTGGG ACTCAATAGA TCTTGATAAT CCACAGGAGA ACATTAAGGC CACCCAGCTC 
CTGGAGGGCC TGGTGCAGGA GCTGCAGAAG AAGGCAGAAC ACCAGGTGGG GGAAGATGGG 
TTTTTGCTGA AGATCAAGCT GGGGCACTAT GCCACGCAGC TCCAGAACAC GTATGACCGC 
TGCCCCATGG AGCTGGTTCG CTGTATCCGG CACATTCTAT ACAATGAACA GAGGCTGGTC 
CGAGAAGCCA ACAACGGAAG CTCTCCGGCT GGAAGTCTTG CTGACGCCAT GTCCCAGAAG 
CACCTTCAGA TCAACCAAAC GTTTGAGGAG CTGCGTCTGA TCACACAGGA CACGGAGAGC 
GAGCTGAAGA AGCTGCAGCA GACCCAAGAG TACTTCATCA TCCAGTACCA GGAGAGCCTA 
CGGATCCAAG CTCAGTTTGC CCAGCTGGCC CAACTGAACC CCCAGGAGCG CATGAGCAGG 
GAGACAGCCC TCCAGCAGAA GCAAGTGTCC CTGGAGACCT GGCTGCAGCG AGAGGCACAG 
ACACTGCAGC AGTACCGCGT GGAGCTGGCT GAAAAGCACC AGAAGACCCT GCAGCTGCTG 
AGGAAGCAGC AGACCATCAT CCTGGACGAC GAGCTGATCC AGTGGAAGCG GCGACAGCAA 
CTGGCCGGGA ATGGGGGTCC TCCTGAGGGC AGCCTGGATG TGCTGCAGTC CTGGTGTGAG 
AAGCTGGCCG AGATCATCTG GCAGAACCGG CAGCAGATCC GCAGGGCTGA GCACCTGTGC 
CAGCAGCTGC CCATCCCAGG CCCCGTGGAG GAGATGCTGG CCGAGGTCAA CGCCACCATC 
ACAGACATCA TCTCAGCCCT GGTCACCAGC ACGTTCATCA TCGAGAAGCA GCCTCCTCAG 
GTCCTGAAGA CCCAGACCAA GTTCGCGGCC ACCGTGCGCC TGCTGGTGGG CGGGAAGCTG 
AACGTGCACA TGAACCCTCC GCAGGTGAAG GCAACCATCA TCAGCGAGCA GCAGGCCAAG 
TCCCTGCTCA AGAACGAGAA CACCCGCAAT GATTATAGCG GCGAGATCCT GAACAACTGC 
TGCGTCATGG AGTACCACCA GGCCACAGGC ACCCTCAGCG CCCACTTCAG AAACATGTCC 
CTGAAACGAA TCAAGAGGTC TGACCGCCGT GGTGCAGAGT CGGTCACGGA AGAGAAGTTC 
ACAATCTTGT TTGACTCACA GTTCAGTGTT GGTGGAAATG AGCTGGTCTT TCAAGTCAAG 
ACCTTGTCGC TCCCGGTGGT GGTGATCGTT CACGGCAGCC AGGACAACAA TGCGACGGCT 
ACCGTCCTCT GGGACAACGC CTTTGCAGAG CCTGGCAGGG TGCCATTTGC CGTGCCTGAC 
AAGGTGCTGT GGCCGCAGCT GTGTGAGGCG CTCAACATGA AGTTCAAGGC TGAGGTGCAG 
AGCAACCGGG GCTTGACCAA GGAGAACCTC GTGTTCCTGG CACAGAAACT GTTCAACAGC 
AGCAGCAACC ACCTCGAGGA CTACAACAGC ATGTCCGTGT CCTGGTCCCA GTTCAACCGG 
GAGAATCTGC CAGGACGGAA TTACACTTTC TGGCAGTGGT TTGACGGTGT GATGGAGGTG 
TTAAAGAAAC ATCTCAAGCC TCACTGGAAC GATGGGGCCA TCTTGGGTTT CGTGAACAAG 
CAGCAGGCCC ACGACCTGCT CATCAACAAG CCAGATGGGA CCTTCCTGCT GCGCTTCAGC 
GACTCGGAAA TCGGGGGCAT CACCATTGCT TGGAAGTTTG ACTCCCAGGA GAGAATGTTT 
TGGAATCTGA TGCCTTTTAC CACAAGAGAC TTCTCTATCC GGTCCCTCGC TGACCGCCTG 
GGGGACCTGA ATTACCTCAT ATACGTGTTT CCTGATCGGC CAAAGGATGA AGTATATTCT 
AAGTACTACA CACCAGTCCC GTGTGAGCCC GCCACCGCGA AAGCAGCTGA CGGATACGTG 
AAGCCGCAGA TCAAGCAAGT GGTCCCCGAG TTTGTCAATG CTTCTGCAGA TGCAGGGAGC 
GGCGCCACCT ACATGGACCA GGCTCCTTCC CCAGTGGTGT GCCCTCAGGC TCACTATAAC 
ATGTACCCAC AGAATCCCGA CTCTGTCCTC GATACCGATG GGGATTTCGA TCTGGAAGAC 
ACGATGGACG TGGCACGGCG TGTGGAAGAG CTCTTAGGCC GGCCCATGGA CAGTCAGTGG 
ATCCCTCACG CGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genotype-dependent effects of TGF-?1 on mast cell function: targeting the Stat5 pathway.
Journal of immunology (Baltimore, Md. : 1950)191(9)4505-13(2013 Nov)
Fernando J,Faber TW,Pullen NA,Falanga YT,Kolawole EM,Oskeritzian CA,Barnstein BO,Bandara G,Li G,Schwartz LB,Spiegel S,Straus DB,Conrad DH,Bunting KD,Ryan JJ

Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3.
American journal of physiology. Gastrointestinal and liver physiology303(12)G1402-15(2012 Dec)
Fernandez DJ,Tuma DJ,Tuma PL

The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.
Molecular and cellular biology32(18)3732-42(2012 Sep)
Krebs CJ,Schultz DC,Robins DM

Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription.
PloS one7(11)e50278(2012)
Varco-Merth B,Mirza K,Alzhanov DT,Chia DJ,Rotwein P

MSM enhances GH signaling via the Jak2/STAT5b pathway in osteoblast-like cells and osteoblast differentiation through the activation of STAT5b in MSCs.
PloS one7(10)e47477(2012)
Joung YH,Lim EJ,Darvin P,Chung SC,Jang JW,Do Park K,Lee HK,Kim HS,Park T,Yang YM