Tpm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpm4
Entrez Gene ID	24852
Full Name	tropomyosin 4
Synonyms	Tpm4.2,Tpm4.2cy
General protein information	Preferred Names tropomyosin 4 Names tropomyosin alpha-4 chain TM-4 tropomycin 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	binds actin filaments; important for calcium-dependent regulation of striated muscle contraction; may be important for stabilizing actin filaments in the cytoskeleton [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012678.2	NP_036810.1	tropomyosin alpha-4 chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100612873	XP_003316217.1
	LOC703057	XP_001092183.1
Mus musculus (house mouse)	Tpm4	NP_001001491.1
Rattus norvegicus (Norway rat)	Tpm4	NP_036810.1
Danio rerio (zebrafish)	tpm4b	NP_955969.1

Gene Ontology
Bibliography

Related articles in PubMed

A systematic nomenclature for mammalian tropomyosin isoforms.
Geeves MA, Hitchcock-DeGregori SE, Gunning PW
Journal of muscle research and cell motility36(2)147-53(2015 Apr)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Tropomyosin isoforms localize to distinct microfilament populations in osteoclasts.
McMichael BK, Kotadiya P, Singh T, Holliday LS, Lee BS
Bone39(4)694-705(2006 Oct)

h2-Calponin is regulated by mechanical tension and modifies the function of actin cytoskeleton.
Hossain MM, Crish JF, Eckert RL, Lin JJ, Jin JP
The Journal of biological chemistry280(51)42442-53(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tpm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00596	NM_012678.2 Latest version!	Rattus norvegicus tropomyosin 4 (Tpm4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpm4 ( NM_012678.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036810.1 Rattus norvegicus tropomyosin 4 (Tpm4), mRNA.

Price & Availability↑

CloneID	ORa00596
Clone ID Related Accession (Same CDS sequence)	NM_012678.2
Accession Version	NM_012678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	tropomyosin alpha-4 chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02780.1. On Aug 31, 2012 this sequence version replaced NM_012678.1. ##Evidence-Data-START## Transcript exon combination :: J02780.1, FQ225054.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCC TCAACTCACT GGAGGCGGTG AAGCGCAAGA TCCAGGCCCT GCAGCAGCAG 
GCGGACGACG CAGAGGACCG TGCGCAGGGC CTGCAGCGCG AGCTGGATGG CGAGCGCGAA 
CGGCGCGAGA AAGCTGAAGG AGATGCGGCC GCTCTCAACC GTCGCATCCA GCTGGTGGAG 
GAAGAGCTGG ACCGGGCTCA GGAGCGACTG GCCACAGCCC TGCAGAAGCT GGAGGAGGCA 
GAGAAGGCTG CTGACGAGAG TGAGAGAGGC ATGAAGGTGA TAGAGAACCG AGCCATGAAA 
GACGAGGAGA AGATGGAGAT CCAGGAGATG CAGCTCAAAG AAGCCAAGCA CATCGCTGAG 
GAGGCTGACC GGAAGTATGA GGAGGTTGCT CGTAAGTTGG TCATCCTGGA GGGTGAGCTG 
GAGAGAGCAG AGGAGCGGGC GGAGGTGTCT GAACTAAAGA GTAGCGACCT GGAAGAGGAG 
CTCAAGAACG TAACTAACAA TCTGAAATCA CTGGAGGCTG CTTCTGAAAA GTACTCTGAA 
AAGGAGGATA AATATGAAGA AGAAATCAAG CTTCTGTCTG ACAAACTGAA AGAGGCTGAG 
ACCCGAGCTG AGTTTGCGGA AAGGACAGTT TCTAAACTGG AGAAGACAAT CGATGACCTG 
GAAGAAAAAC TTGCCCAGGC CAAAGAAGAG AACGTGGGCT TGCATCAGAC ACTGGACCAG 
ACACTAAACG AACTTAACTG TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036810.1
CDS	24..770
Translation	MAGLNSLEAVKRKIQALQQQADDAEDRAQGLQRELDGERERREKAEGDAAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKSSDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVSKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI

Target ORF information:

RefSeq Version	NM_012678.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tropomyosin 4 (Tpm4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012678.2

ORF Insert Sequence:

ATGGCCGGCC TCAACTCACT GGAGGCGGTG AAGCGCAAGA TCCAGGCCCT GCAGCAGCAG 
GCGGACGACG CAGAGGACCG TGCGCAGGGC CTGCAGCGCG AGCTGGATGG CGAGCGCGAA 
CGGCGCGAGA AAGCTGAAGG AGATGCGGCC GCTCTCAACC GTCGCATCCA GCTGGTGGAG 
GAAGAGCTGG ACCGGGCTCA GGAGCGACTG GCCACAGCCC TGCAGAAGCT GGAGGAGGCA 
GAGAAGGCTG CTGACGAGAG TGAGAGAGGC ATGAAGGTGA TAGAGAACCG AGCCATGAAA 
GACGAGGAGA AGATGGAGAT CCAGGAGATG CAGCTCAAAG AAGCCAAGCA CATCGCTGAG 
GAGGCTGACC GGAAGTATGA GGAGGTTGCT CGTAAGTTGG TCATCCTGGA GGGTGAGCTG 
GAGAGAGCAG AGGAGCGGGC GGAGGTGTCT GAACTAAAGA GTAGCGACCT GGAAGAGGAG 
CTCAAGAACG TAACTAACAA TCTGAAATCA CTGGAGGCTG CTTCTGAAAA GTACTCTGAA 
AAGGAGGATA AATATGAAGA AGAAATCAAG CTTCTGTCTG ACAAACTGAA AGAGGCTGAG 
ACCCGAGCTG AGTTTGCGGA AAGGACAGTT TCTAAACTGG AGAAGACAAT CGATGACCTG 
GAAGAAAAAC TTGCCCAGGC CAAAGAAGAG AACGTGGGCT TGCATCAGAC ACTGGACCAG 
ACACTAAACG AACTTAACTG TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systematic nomenclature for mammalian tropomyosin isoforms.
Journal of muscle research and cell motility36(2)147-53(2015 Apr)
Geeves MA,Hitchcock-DeGregori SE,Gunning PW

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Tropomyosin isoforms localize to distinct microfilament populations in osteoclasts.
Bone39(4)694-705(2006 Oct)
McMichael BK,Kotadiya P,Singh T,Holliday LS,Lee BS

h2-Calponin is regulated by mechanical tension and modifies the function of actin cytoskeleton.
The Journal of biological chemistry280(51)42442-53(2005 Dec)
Hossain MM,Crish JF,Eckert RL,Lin JJ,Jin JP