Vamp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vamp2
Entrez Gene ID	24803
Full Name	vesicle-associated membrane protein 2
Synonyms	RATVAMPB,RATVAMPIR,SYB,Syb2
General protein information	Preferred Names vesicle-associated membrane protein 2 Names vesicle-associated membrane protein 2 Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2) VAMP-2 Vesicle-associated membrane protein (synaptobrevin 2) synaptobrevin-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	plays a role in membrane fusion in neuronal exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246593.3	XP_006246655.1	vesicle-associated membrane protein 2 isoform X1
NM_012663.2	NP_036795.1	vesicle-associated membrane protein 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VAMP2	NP_776908.1
Drosophila melanogaster (fruit fly)	n-syb	NP_001261270.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005507	XP_001688670.1
Pan troglodytes (chimpanzee)	VAMP2	XP_001156324.1
Mus musculus (house mouse)	Vamp2	NP_033523.1
Danio rerio (zebrafish)	vamp2	NP_956299.1
	VAMP2	NP_001027992.1
Canis lupus familiaris (dog)	VAMP2	XP_005620068.1
Rattus norvegicus (Norway rat)	Vamp2	NP_036795.1
Gallus gallus (chicken)	VAMP2	XP_422640.2
Xenopus tropicalis (tropical clawed frog)	vamp2	NP_989031.1
Homo sapiens (human)	VAMP2	NP_055047.2

Gene Ontology
Bibliography

Related articles in PubMed

An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.
Jakhanwal S, Lee CT, Urlaub H, Jahn R
The EMBO journal36(12)1788-1802(2017 06)

A Central Small Amino Acid in the VAMP2 Transmembrane Domain Regulates the Fusion Pore in Exocytosis.
Hastoy B, Scotti PA, Milochau A, Fezoua-Boubegtiten Z, Rodas J, Megret R, Desbat B, Laguerre M, Castano S, Perrais D, Rorsman P, Oda R, Lang J
Scientific reports7(1)2835(2017 Jun)

Synaptobrevin transmembrane domain determines the structure and dynamics of the SNARE motif and the linker region.
Han J, Pluhackova K, Bruns D, B?ckmann RA
Biochimica et biophysica acta1858(4)855-65(2016 Apr)

Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.
Rajappa R, Gauthier-Kemper A, B?ning D, H?ve J, Klingauf J
Cell reports14(6)1369-1381(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ternary complex of syntaxin1:SNAP25:Munc18-1binds synaptobrevin with fast kinetics, resulting in the rapid formation of a fully zippered SNARE complex to which Munc18-1 remains tethered by the N-terminal domain of syntaxin1.
Title: An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.

These effects weaken the integrity of the outer membrane layer and are attributed mainly to the highly charged linker and juxtamembrane regions of sybII
Title: Synaptobrevin transmembrane domain determines the structure and dynamics of the SNARE motif and the linker region.

Syp1 clears Syb2 from the presynaptic active zone to prevent short-term depression.
Title: Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.

miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein.
Title: MicroRNA-206 regulates surfactant secretion by targeting VAMP-2.

In vivo silencing of VAMP2 but not VAMP3 in TALs blunted cAMP-stimulated steady-state surface NKCC2 expression and completely blocked cAMP-stimulated NKCC2 exocytic delivery
Title: Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs.

The following Vamp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vamp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00562	NM_012663.2 Latest version!	Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa33234	XM_006246593.3 Latest version!	Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vamp2 ( NM_012663.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036795.1 Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), mRNA.

Price & Availability↑

CloneID	ORa00562
Clone ID Related Accession (Same CDS sequence)	NM_012663.2
Accession Version	NM_012663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-associated membrane protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074003.1. On Jul 8, 2004 this sequence version replaced NM_012663.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC074003.1, M24105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGCTA CCGCTGCCAC CGTCCCGCCT GCCGCCCCGG CCGGCGAGGG TGGCCCCCCT 
GCACCTCCTC CAAATCTTAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAATGTG GACAAGGTCC TGGAGCGGGA CCAGAAGCTA 
TCGGAACTGG ATGATCGCGC AGATGCCCTC CAGGCAGGGG CCTCCCAGTT TGAAACAAGT 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATCGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036795.1
CDS	83..433
Translation	MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST

Target ORF information:

RefSeq Version	NM_012663.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012663.2

ORF Insert Sequence:

ATGTCGGCTA CCGCTGCCAC CGTCCCGCCT GCCGCCCCGG CCGGCGAGGG TGGCCCCCCT 
GCACCTCCTC CAAATCTTAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAATGTG GACAAGGTCC TGGAGCGGGA CCAGAAGCTA 
TCGGAACTGG ATGATCGCGC AGATGCCCTC CAGGCAGGGG CCTCCCAGTT TGAAACAAGT 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATCGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp2 ( XM_006246593.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246655.1 Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33234
Clone ID Related Accession (Same CDS sequence)	XM_006246593.3
Accession Version	XM_006246593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-associated membrane protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCTA CCGCTGCCAC CGTCCCGCCT GCCGCCCCGG CCGGCGAGGG TGGCCCCCCT 
GCACCTCCTC CAAATCTTAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAATGTG GACAAGGTCC TGGAGCGGGA CCAGAAGCTA 
TCGGAACTGG ATGATCGCGC AGATGCCCTC CAGGCAGGGG CCTCCCAGTT TGAAACAAGT 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATCGGTGAGT GGAGCAGGAG TGGCCAGGGC 
CCTTTCCCTG GAGAAGTAGA GGGTTTCCCT GTGGAGTCGG GGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246655.1
CDS	449..856
Translation	MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIGEWSRSGQGPFPGEVEGFPVESGL

Target ORF information:

RefSeq Version	XM_006246593.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vesicle-associated membrane protein 2 (Vamp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246593.3

ORF Insert Sequence:

ATGTCGGCTA CCGCTGCCAC CGTCCCGCCT GCCGCCCCGG CCGGCGAGGG TGGCCCCCCT 
GCACCTCCTC CAAATCTTAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAATGTG GACAAGGTCC TGGAGCGGGA CCAGAAGCTA 
TCGGAACTGG ATGATCGCGC AGATGCCCTC CAGGCAGGGG CCTCCCAGTT TGAAACAAGT 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATCGGTGAGT GGAGCAGGAG TGGCCAGGGC 
CCTTTCCCTG GAGAAGTAGA GGGTTTCCCT GTGGAGTCGG GGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.
The EMBO journal36(12)1788-1802(2017 06)
Jakhanwal S,Lee CT,Urlaub H,Jahn R

A Central Small Amino Acid in the VAMP2 Transmembrane Domain Regulates the Fusion Pore in Exocytosis.
Scientific reports7(1)2835(2017 Jun)
Hastoy B,Scotti PA,Milochau A,Fezoua-Boubegtiten Z,Rodas J,Megret R,Desbat B,Laguerre M,Castano S,Perrais D,Rorsman P,Oda R,Lang J

Synaptobrevin transmembrane domain determines the structure and dynamics of the SNARE motif and the linker region.
Biochimica et biophysica acta1858(4)855-65(2016 Apr)
Han J,Pluhackova K,Bruns D,B?ckmann RA

Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.
Cell reports14(6)1369-1381(2016 Feb)
Rajappa R,Gauthier-Kemper A,B?ning D,H?ve J,Klingauf J