Fev cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fev
Entrez Gene ID	246271
Full Name	FEV, ETS transcription factor
Synonyms	Pet1
General protein information	Preferred Names FEV, ETS transcription factor Names protein FEV ETS domain transcription factor Pet-1 FEV (ETS oncogene family) PC12 ETS domain-containing transcription factor 1 PC12 ETS factor 1 pet-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33
Summary	transcriptional regulator of downstream target genes involved in cholinergic neurotransmission [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_144753.2	NP_653354.2	protein FEV

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FEV	XP_003309530.1
	FEV	XP_001095962.1
Bos taurus (cattle)	FEV	NP_001179532.1
Danio rerio (zebrafish)	fev	NP_001074126.1
Xenopus tropicalis (tropical clawed frog)	fev	XP_002934005.1
Rattus norvegicus (Norway rat)	Fev	NP_653354.2
Homo sapiens (human)	FEV	NP_059991.1
Mus musculus (house mouse)	Fev	NP_694751.1
Gallus gallus (chicken)	LOC100857480	XP_003641694.1

Gene Ontology
Bibliography

Related articles in PubMed

Pet-1 is required across different stages of life to regulate serotonergic function.
Liu C, Maejima T, Wyler SC, Casadesus G, Herlitze S, Deneris ES
Nature neuroscience13(10)1190-8(2010 Oct)

Estradiol increases Pet-1 and serotonin transporter mRNA in the midbrain raphe nuclei of ovariectomized rats.
Rivera HM, Oberbeck DR, Kwon B, Houpt TA, Eckel LA
Brain research125951-8(2009 Mar)

mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons.
Pfaar H, von Holst A, Vogt Weisenhorn DM, Brodski C, Guimera J, Wurst W
Development genes and evolution212(1)43-6(2002 Feb)

Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription.
Fyodorov D, Nelson T, Deneris E
Journal of neurobiology34(2)151-63(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that a physiologically relevant regimen of estradiol treatment in ovariectomized rats increases Pet-1 and 5-HTT mRNA levels in the midbrain raphe nuclei at a time when the anorexigenic effect of estradiol is apparent.
Title: Estradiol increases Pet-1 and serotonin transporter mRNA in the midbrain raphe nuclei of ovariectomized rats.

The following Fev gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fev cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02496	NM_144753.2 Latest version!	Rattus norvegicus FEV transcription factor, ETS family member (Fev), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fev ( NM_144753.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_653354.2 Rattus norvegicus FEV transcription factor, ETS family member (Fev), mRNA.

Price & Availability↑

CloneID	ORa02496
Clone ID Related Accession (Same CDS sequence)	NM_144753.2
Accession Version	NM_144753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	protein FEV
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U91679.1. On Jan 26, 2008 this sequence version replaced NM_144753.1. Sequence Note: The sequence U91679.1 is from a chimeric clone. Only the Fev sequence region was propagated into this RefSeq record. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906348, SAMN01906349 [ECO:0000350] ##Evidence-Data-END##

ATGAGACAGA GCGGCACCTC CCAGCCCCTG CTGATCAACA TGTACCTACC AGATCCCGTC 
GGAGATGGTC TTTTTAAGGA AGGGAAGAGC CCGAGCTGGG GGCCGCTGAG CCCTGCGGTA 
CAGAAAGGCA GCGGGCAGAT CCAGTTGTGG CAGTTTCTAC TGGAGCTGCT GGCAGACCGC 
GCGAACGCCG GCTGCATCGC GTGGGAGGGC GGCCACGGCG AGTTCAAGCT CACCGACCCC 
GACGAGGTGG CGCGACGCTG GGGCGAGCGC AAGAGCAAGC CCAATATGAA CTACGACAAG 
CTAAGTCGAG CACTGCGCTA CTACTACGAC AAAAACATCA TGAGCAAGGT GCACGGCAAG 
CGCTACGCCT ACCGCTTTGA CTTCCAGGGC CTGGCACAGG CTTGCCAGCC ACCACCCGCG 
CACGCCCACG CCGCCGCTGC CGCCGCCGCA GCGGCAGCCG CCGCCCAGGA TGGCGCACTT 
TACAAGCTCC CGGCTGGTCT GGCTCCACTG CCCTTCCCCG GCCTCTCCAA ACTCAACCTT 
ATGGCAGCCT CGGCCGGCGT GGCGCCCGCT GGCTTCTCTT ACTGGCCTGG TCCCAACGCC 
ACCGCCGCTG CCGCCGCCAC CGCTGCGCTC TACCCAACCC CGGGCTTGCA GCCCCCTCCC 
GGGCCCTTTG GCGCGGTGGC CGCCGCTTCG CACTTGGGGG GTCATTATCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653354.2
CDS	184..897
Translation	MRQSGTSQPLLINMYLPDPVGDGLFKEGKSPSWGPLSPAVQKGSGQIQLWQFLLELLADRANAGCIAWEGGHGEFKLTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMSKVHGKRYAYRFDFQGLAQACQPPPAHAHAAAAAAAAAAAAQDGALYKLPAGLAPLPFPGLSKLNLMAASAGVAPAGFSYWPGPNATAAAAATAALYPTPGLQPPPGPFGAVAAASHLGGHYH

Target ORF information:

RefSeq Version	NM_144753.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FEV transcription factor, ETS family member (Fev), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144753.2

ORF Insert Sequence:

ATGAGACAGA GCGGCACCTC CCAGCCCCTG CTGATCAACA TGTACCTACC AGATCCCGTC 
GGAGATGGTC TTTTTAAGGA AGGGAAGAGC CCGAGCTGGG GGCCGCTGAG CCCTGCGGTA 
CAGAAAGGCA GCGGGCAGAT CCAGTTGTGG CAGTTTCTAC TGGAGCTGCT GGCAGACCGC 
GCGAACGCCG GCTGCATCGC GTGGGAGGGC GGCCACGGCG AGTTCAAGCT CACCGACCCC 
GACGAGGTGG CGCGACGCTG GGGCGAGCGC AAGAGCAAGC CCAATATGAA CTACGACAAG 
CTAAGTCGAG CACTGCGCTA CTACTACGAC AAAAACATCA TGAGCAAGGT GCACGGCAAG 
CGCTACGCCT ACCGCTTTGA CTTCCAGGGC CTGGCACAGG CTTGCCAGCC ACCACCCGCG 
CACGCCCACG CCGCCGCTGC CGCCGCCGCA GCGGCAGCCG CCGCCCAGGA TGGCGCACTT 
TACAAGCTCC CGGCTGGTCT GGCTCCACTG CCCTTCCCCG GCCTCTCCAA ACTCAACCTT 
ATGGCAGCCT CGGCCGGCGT GGCGCCCGCT GGCTTCTCTT ACTGGCCTGG TCCCAACGCC 
ACCGCCGCTG CCGCCGCCAC CGCTGCGCTC TACCCAACCC CGGGCTTGCA GCCCCCTCCC 
GGGCCCTTTG GCGCGGTGGC CGCCGCTTCG CACTTGGGGG GTCATTATCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pet-1 is required across different stages of life to regulate serotonergic function.
Nature neuroscience13(10)1190-8(2010 Oct)
Liu C,Maejima T,Wyler SC,Casadesus G,Herlitze S,Deneris ES

Estradiol increases Pet-1 and serotonin transporter mRNA in the midbrain raphe nuclei of ovariectomized rats.
Brain research125951-8(2009 Mar)
Rivera HM,Oberbeck DR,Kwon B,Houpt TA,Eckel LA

mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons.
Development genes and evolution212(1)43-6(2002 Feb)
Pfaar H,von Holst A,Vogt Weisenhorn DM,Brodski C,Guimera J,Wurst W

Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription.
Journal of neurobiology34(2)151-63(1998 Feb)
Fyodorov D,Nelson T,Deneris E