Pde4d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde4d
Entrez Gene ID	24627
Full Name	phosphodiesterase 4D
Synonyms	DPDE3,PDE3/IVd
General protein information	Preferred Names phosphodiesterase 4D Names cAMP-specific 3',5'-cyclic phosphodiesterase 4D 3',5'-cyclic AMP phosphodiesterase cAMP-specific phosphodiesterase PDE4D cAMP-specific phosphodiesterase splice variant PDE4D8 phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q14
Summary	may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017032.1	NP_058728.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 5
NM_001113328.1	NP_001106799.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 1
XM_008760723.1	XP_008758945.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X3
NM_001113332.1	NP_001106803.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 3
XM_008760721.1	XP_008758943.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X1
XM_008760722.2	XP_008758944.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X2
NM_001113329.1	NP_001106800.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 2
NM_001113334.1	NP_001106805.1	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pde4d	NP_001106803.1
Danio rerio (zebrafish)	pde4d	XP_695374.5
Drosophila melanogaster (fruit fly)	dnc	NP_726858.2
Pan troglodytes (chimpanzee)	PDE4D	XP_001138610.1
	PDE4D	XP_002804419.1
Bos taurus (cattle)	PDE4D	XP_003587560.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000236	XP_003436946.1
Homo sapiens (human)	PDE4D	NP_001184147.1
Canis lupus familiaris (dog)	PDE4D	XP_003433851.1
Gallus gallus (chicken)	PDE4D	XP_003643033.2
Caenorhabditis elegans (roundworm)	pde-4	NP_871945.1
Xenopus tropicalis (tropical clawed frog)	pde4d	XP_002938226.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterologous desensitization of cardiac ?-adrenergic signal via hormone-induced ?AR/arrestin/PDE4 complexes.
Shi Q, Li M, Mika D, Fu Q, Kim S, Phan J, Shen A, Vandecasteele G, Xiang YK
Cardiovascular research113(6)656-670(2017 May)

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.
Kim MJ, Park SK, Lee JH, Jung CY, Sung DJ, Park JH, Yoon YS, Park J, Park KG, Song DK, Cho H, Kim ST, Koo SH
Diabetes64(9)3189-202(2015 Sep)

Heterozygous mutations in cyclic AMP phosphodiesterase-4D (PDE4D) and protein kinase A (PKA) provide new insights into the molecular pathology of acrodysostosis.
Kaname T, Ki CS, Niikawa N, Baillie GS, Day JP, Yamamura K, Ohta T, Nishimura G, Mastuura N, Kim OH, Sohn YB, Kim HW, Cho SY, Ko AR, Lee JY, Kim HW, Ryu SH, Rhee H, Yang KS, Joo K, Lee J, Kim CH, Cho KH, Kim D, Yanagi K, Naritomi K, Yoshiura K, Kondoh T, Nii E, Tonoki H, Houslay MD, Jin DK
Cellular signalling26(11)2446-59(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIK1 serves as a part of a self-regulatory circuit to modulate insulin secretion from pancreatic beta-cells by controlling cAMP concentration through modulation of PDE4D activity.
Title: Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.

specific inhibitory PDE4D mutations can lead to the molecular pathology of acrodysostosis without hormone resistance but that the pathological phenotype may well be dependent on an over-compensatory induction of other PDE4 isoforms
Title: Heterozygous mutations in cyclic AMP phosphodiesterase-4D (PDE4D) and protein kinase A (PKA) provide new insights into the molecular pathology of acrodysostosis.

PDE4D9 was found to locate primarily not only in the perinuclear region of Rat-1 cells but also at the cell margins.
Title: Mitotic activation of the DISC1-inducible cyclic AMP phosphodiesterase-4D9 (PDE4D9), through multi-site phosphorylation, influences cell cycle progression.

Inhibition of PDE4D activity decreases hepatic inflammation, injury and fibrosis.
Title: Rolipram attenuates bile duct ligation-induced liver injury in rats: a potential pathogenic role of PDE4.

PHD2 may directly interact with PDE4D to function as a novel regulator of the intracellular cAMP levels in cardiomyocytes.
Title: Prolyl hydroxylase domain protein 2 regulates the intracellular cyclic AMP level in cardiomyocytes through its interaction with phosphodiesterase 4D.

The following Pde4d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde4d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02569	NM_017032.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00
ORa42934	XM_008760722.2 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa42935	XM_008760723.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa00935	NM_001113329.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa00940	NM_001113334.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa39888	NM_001113328.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa42757	NM_001113332.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
ORa42933	XM_008760721.1 Latest version!	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde4d ( NM_017032.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058728.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 5, mRNA.

Price & Availability↑

CloneID	ORa02569
Clone ID Related Accession (Same CDS sequence)	NM_017032.1
Accession Version	NM_017032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U09457.1. Transcript Variant: This variant (5) has an alternate 5' coding exon, as compared to variant 1. The resulting isoform (5) has a shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATGCATG TGAATACCTT CCCTTTCAGA AGGCATTCCT GGATATGTTT CGATGTGGAC 
AATGGCACAT CAGCGGGACG GAGTCCCTTG GATCCCATGA CCAGCCCAGG GTCTGGGCTA 
ATTCTCCAAG CAAACTTTGT GCACAGTCAA CGCCGGGAGT CCTTCCTGTA TCGATCAGAC 
AGCGATTATG ACCTCTCTCC AAAGTCTATG TCCAGGAACT CCTCGATTGC CAGTGATATA 
CATGGAGATG ACTTGATTGT GACTCCATTT GCTCAGGTCT TGGCCAGCCT TCGAACTGTA 
AGAAACAACT TTGCTGCATT AACTAATTTG CAAGATCGCG CACCCAGCAA AAGATCACCC 
ATGTGCAACC AACCATCCAT CAACAAAGCC ACCATCACAG AGGAGGCCTA CCAGAAACTG 
GCCAGCGAGA CCCTGGAGGA GCTGGACTGG TGTCTGGACC AGCTAGAGAC CCTGCAGACC 
AGGCACTCCG TCAGTGAGAT GGCCTCCAAC AAGTTCAAGA GGATGCTTAA TCGGGAGCTC 
ACCCACCTCT CTGAAATGAG TCGGTCTGGC AACCAGGTGT CGGAGTACAT ATCAAACACG 
TTCTTAGATA AGCAACATGA AGTAGAAATT CCCTCTCCGA CTCAGAAGGA AAAAGAGAAG 
AAGAAAAGGC CGATGTCACA GATCAGTGGG GTCAAGAAGT TGATGCACAG CTCCAGCCTG 
ACCAATTCCT GCATTCCAAG ATTTGGGGTT AAAACAGAGC AGGAAGATGT CCTGGCCAAG 
GAACTAGAAG ACGTGAACAA GTGGGGCCTC CACGTTTTCC GAATAGCGGA GCTGTCTGGC 
AACCGGCCTC TGACTGTTAT CATGCACACC ATTTTTCAGG AACGAGATTT GTTAAAAACG 
TTTAAAATCC CAGTGGACAC TTTGATTACG TATCTTATGA CTCTAGAAGA CCATTACCAT 
GCTGACGTGG CCTATCACAA CAACATCCAT GCTGCAGATG TCGTCCAGTC AACTCATGTG 
CTGCTCTCTA CACCCGCTTT GGAGGCTGTT TTCACTGACT TGGAGATTCT CGCGGCCATT 
TTTGCCAGTG CAATACATGA TGTGGATCAT CCTGGTGTGT CAAATCAATT TCTGATCAAT 
ACAAACTCGG AACTTGCCTT GATGTACAAC GACTCCTCCG TCTTAGAGAA TCATCATTTG 
GCTGTGGGCT TTAAGTTGCT CCAGGAAGAA AACTGTGACA TTTTCCAGAA TCTGACCAAA 
AAGCAAAGAC AATCTTTAAG GAAAATGGCC ATTGACATTG TACTAGCGAC AGACATGTCA 
AAGCACATGA ATCTGCTGGC TGATCTGAAA ACAATGGTTG AAACGAAGAA GGTGACGAGC 
TCTGGCGTCC TCCTCCTTGA TAACTATTCT GACAGGATCC AGGTCCTCCA GAATATGGTG 
CACTGTGCAG ACCTGAGCAA CCCCACAAAG CCACTCCAGC TCTACCGCCA GTGGACGGAC 
CGGATAATGG AGGAGTTCTT CCGTCAGGGG GACCGGGAGC GTGAGCGTGG CATGGAGATA 
AGTCCCATGT GTGACAAGCA CAACGCCTCT GTGGAAAAAT CACAGGTGGG CTTCATAGAC 
TACATTGTTC ATCCACTCTG GGAGACATGG GCAGACCTCG TACATCCCGA CGCCCAGGAC 
ATTTTGGACA CTTTGGAGGA CAATCGTGAG TGGTACCAGA GCACAATCCC CCAGAGCCCC 
TCCCCTGCAC CTGATGACCA AGAGGACGGC CGTCAGGGAC AGACTGAAAA ATTCCAGTTC 
GAACTAACCT TAGAGGAAGA TGGCGAGTCA GACACTGAAA AGGACAGTGG AAGTCAAGTG 
GAGGAAGACA CTAGCTGCAG TGACTCTAAG ACTCTGTGCA CCCAAGACTC AGAGTCCACT 
GAAATTCCCC TTGACGAGCA GGTTGAAGAG GAGGCTGTAG CAGAAGAGGA AAGCCAGCCC 
CAAACTGGCG TCGCAGACGA TTGTTGTCCT GACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058728.1
CDS	1..2019
Translation	MMHVNTFPFRRHSWICFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	NM_017032.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017032.1

ORF Insert Sequence:

ATGATGCATG TGAATACCTT CCCTTTCAGA AGGCATTCCT GGATATGTTT CGATGTGGAC 
AATGGCACAT CAGCGGGACG GAGTCCCTTG GATCCCATGA CCAGCCCAGG GTCTGGGCTA 
ATTCTCCAAG CAAACTTTGT GCACAGTCAA CGCCGGGAGT CCTTCCTGTA TCGATCAGAC 
AGCGATTATG ACCTCTCTCC AAAGTCTATG TCCAGGAACT CCTCGATTGC CAGTGATATA 
CATGGAGATG ACTTGATTGT GACTCCATTT GCTCAGGTCT TGGCCAGCCT TCGAACTGTA 
AGAAACAACT TTGCTGCATT AACTAATTTG CAAGATCGCG CACCCAGCAA AAGATCACCC 
ATGTGCAACC AACCATCCAT CAACAAAGCC ACCATCACAG AGGAGGCCTA CCAGAAACTG 
GCCAGCGAGA CCCTGGAGGA GCTGGACTGG TGTCTGGACC AGCTAGAGAC CCTGCAGACC 
AGGCACTCCG TCAGTGAGAT GGCCTCCAAC AAGTTCAAGA GGATGCTTAA TCGGGAGCTC 
ACCCACCTCT CTGAAATGAG TCGGTCTGGC AACCAGGTGT CGGAGTACAT ATCAAACACG 
TTCTTAGATA AGCAACATGA AGTAGAAATT CCCTCTCCGA CTCAGAAGGA AAAAGAGAAG 
AAGAAAAGGC CGATGTCACA GATCAGTGGG GTCAAGAAGT TGATGCACAG CTCCAGCCTG 
ACCAATTCCT GCATTCCAAG ATTTGGGGTT AAAACAGAGC AGGAAGATGT CCTGGCCAAG 
GAACTAGAAG ACGTGAACAA GTGGGGCCTC CACGTTTTCC GAATAGCGGA GCTGTCTGGC 
AACCGGCCTC TGACTGTTAT CATGCACACC ATTTTTCAGG AACGAGATTT GTTAAAAACG 
TTTAAAATCC CAGTGGACAC TTTGATTACG TATCTTATGA CTCTAGAAGA CCATTACCAT 
GCTGACGTGG CCTATCACAA CAACATCCAT GCTGCAGATG TCGTCCAGTC AACTCATGTG 
CTGCTCTCTA CACCCGCTTT GGAGGCTGTT TTCACTGACT TGGAGATTCT CGCGGCCATT 
TTTGCCAGTG CAATACATGA TGTGGATCAT CCTGGTGTGT CAAATCAATT TCTGATCAAT 
ACAAACTCGG AACTTGCCTT GATGTACAAC GACTCCTCCG TCTTAGAGAA TCATCATTTG 
GCTGTGGGCT TTAAGTTGCT CCAGGAAGAA AACTGTGACA TTTTCCAGAA TCTGACCAAA 
AAGCAAAGAC AATCTTTAAG GAAAATGGCC ATTGACATTG TACTAGCGAC AGACATGTCA 
AAGCACATGA ATCTGCTGGC TGATCTGAAA ACAATGGTTG AAACGAAGAA GGTGACGAGC 
TCTGGCGTCC TCCTCCTTGA TAACTATTCT GACAGGATCC AGGTCCTCCA GAATATGGTG 
CACTGTGCAG ACCTGAGCAA CCCCACAAAG CCACTCCAGC TCTACCGCCA GTGGACGGAC 
CGGATAATGG AGGAGTTCTT CCGTCAGGGG GACCGGGAGC GTGAGCGTGG CATGGAGATA 
AGTCCCATGT GTGACAAGCA CAACGCCTCT GTGGAAAAAT CACAGGTGGG CTTCATAGAC 
TACATTGTTC ATCCACTCTG GGAGACATGG GCAGACCTCG TACATCCCGA CGCCCAGGAC 
ATTTTGGACA CTTTGGAGGA CAATCGTGAG TGGTACCAGA GCACAATCCC CCAGAGCCCC 
TCCCCTGCAC CTGATGACCA AGAGGACGGC CGTCAGGGAC AGACTGAAAA ATTCCAGTTC 
GAACTAACCT TAGAGGAAGA TGGCGAGTCA GACACTGAAA AGGACAGTGG AAGTCAAGTG 
GAGGAAGACA CTAGCTGCAG TGACTCTAAG ACTCTGTGCA CCCAAGACTC AGAGTCCACT 
GAAATTCCCC TTGACGAGCA GGTTGAAGAG GAGGCTGTAG CAGAAGAGGA AAGCCAGCCC 
CAAACTGGCG TCGCAGACGA TTGTTGTCCT GACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( XM_008760722.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758944.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa42934
Clone ID Related Accession (Same CDS sequence)	XM_008760722.2
Accession Version	XM_008760722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG CAAACTATTT ATTGTCAGTG TCTTGGGGCT ACATAAAGTT CAAGAGGATG 
CTTAATCGGG AGCTCACCCA CCTCTCTGAA ATGAGTCGGT CTGGCAACCA GGTGTCGGAG 
TACATATCAA ACACGTTCTT AGATAAGCAA CATGAAGTAG AAATTCCCTC TCCGACTCAG 
AAGGAAAAAG AGAAGAAGAA AAGGCCGATG TCACAGATCA GTGGGGTCAA GAAGTTGATG 
CACAGCTCCA GCCTGACCAA TTCCTGCATC CCAAGATTTG GGGTTAAAAC AGAGCAGGAA 
GATGTCCTGG CCAAGGAACT AGAAGACGTG AACAAGTGGG GCCTCCACGT TTTCCGAATA 
GCGGAGCTGT CTGGCAACCG GCCTCTGACT GTTATCATGC ACACCATTTT TCAGGAACGA 
GATTTGTTAA AAACGTTTAA AATCCCAGTG GACACTTTGA TTACGTATCT TATGACTCTA 
GAAGACCATT ACCATGCTGA CGTGGCCTAT CACAACAACA TCCATGCTGC AGATGTCGTC 
CAGTCAACTC ATGTGCTGCT CTCTACACCC GCTTTGGAGG CTGTTTTCAC TGACTTGGAG 
ATTCTCGCGG CCATTTTTGC CAGTGCAATA CATGATGTGG ATCATCCTGG TGTGTCAAAT 
CAATTTCTGA TCAATACAAA CTCGGAACTT GCCTTGATGT ACAACGACTC CTCCGTCTTA 
GAGAATCATC ATTTGGCTGT GGGCTTTAAG TTGCTCCAGG AAGAAAACTG TGACATTTTC 
CAGAATCTGA CCAAAAAGCA AAGACAATCT TTAAGGAAAA TGGCCATTGA CATTGTACTA 
GCGACAGACA TGTCAAAGCA CATGAATCTG CTGGCTGATC TGAAAACAAT GGTTGAAACG 
AAGAAGGTGA CGAGCTCTGG CGTCCTCCTC CTTGATAACT ATTCTGACAG GATCCAGGTC 
CTCCAGAATA TGGTGCACTG TGCAGACCTG AGCAACCCCA CAAAGCCACT CCAGCTCTAC 
CGCCAGTGGA CGGACCGGAT AATGGAGGAG TTCTTCCGTC AGGGGGACCG GGAGCGTGAG 
CGTGGCATGG AGATAAGTCC CATGTGTGAC AAGCACAACG CCTCTGTGGA AAAATCACAG 
GTGGGCTTCA TAGACTATAT TGTTCATCCA CTCTGGGAGA CATGGGCAGA CCTCGTACAT 
CCCGACGCCC AGGACATTTT GGACACTTTG GAGGACAATC GTGAGTGGTA CCAGAGCACA 
ATCCCCCAGA GCCCCTCCCC TGCACCTGAT GACCAAGAGG ACGGCCGTCA GGGACAGACT 
GAAAAATTCC AGTTCGAACT AACCTTAGAG GAAGATGGCG AGTCAGACAC TGAAAAGGAC 
AGTGGAAGTC AAGTGGAGGA AGACACTAGC TGCAGTGACT CTAAGACTCT GTGCACCCAA 
GACTCAGAGT CCACTGAAAT TCCCCTTGAC GAGCAGGTTG AAGAGGAGGC TGTAGCAGAA 
GAGGAAAGCC AGCCCCAAAC TGGCGTCGCA GACGATTGTT GTCCTGACAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758944.1
CDS	8296..9849
Translation	MPEANYLLSVSWGYIKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	XM_008760722.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760722.2

ORF Insert Sequence:

ATGCCTGAAG CAAACTATTT ATTGTCAGTG TCTTGGGGCT ACATAAAGTT CAAGAGGATG 
CTTAATCGGG AGCTCACCCA CCTCTCTGAA ATGAGTCGGT CTGGCAACCA GGTGTCGGAG 
TACATATCAA ACACGTTCTT AGATAAGCAA CATGAAGTAG AAATTCCCTC TCCGACTCAG 
AAGGAAAAAG AGAAGAAGAA AAGGCCGATG TCACAGATCA GTGGGGTCAA GAAGTTGATG 
CACAGCTCCA GCCTGACCAA TTCCTGCATC CCAAGATTTG GGGTTAAAAC AGAGCAGGAA 
GATGTCCTGG CCAAGGAACT AGAAGACGTG AACAAGTGGG GCCTCCACGT TTTCCGAATA 
GCGGAGCTGT CTGGCAACCG GCCTCTGACT GTTATCATGC ACACCATTTT TCAGGAACGA 
GATTTGTTAA AAACGTTTAA AATCCCAGTG GACACTTTGA TTACGTATCT TATGACTCTA 
GAAGACCATT ACCATGCTGA CGTGGCCTAT CACAACAACA TCCATGCTGC AGATGTCGTC 
CAGTCAACTC ATGTGCTGCT CTCTACACCC GCTTTGGAGG CTGTTTTCAC TGACTTGGAG 
ATTCTCGCGG CCATTTTTGC CAGTGCAATA CATGATGTGG ATCATCCTGG TGTGTCAAAT 
CAATTTCTGA TCAATACAAA CTCGGAACTT GCCTTGATGT ACAACGACTC CTCCGTCTTA 
GAGAATCATC ATTTGGCTGT GGGCTTTAAG TTGCTCCAGG AAGAAAACTG TGACATTTTC 
CAGAATCTGA CCAAAAAGCA AAGACAATCT TTAAGGAAAA TGGCCATTGA CATTGTACTA 
GCGACAGACA TGTCAAAGCA CATGAATCTG CTGGCTGATC TGAAAACAAT GGTTGAAACG 
AAGAAGGTGA CGAGCTCTGG CGTCCTCCTC CTTGATAACT ATTCTGACAG GATCCAGGTC 
CTCCAGAATA TGGTGCACTG TGCAGACCTG AGCAACCCCA CAAAGCCACT CCAGCTCTAC 
CGCCAGTGGA CGGACCGGAT AATGGAGGAG TTCTTCCGTC AGGGGGACCG GGAGCGTGAG 
CGTGGCATGG AGATAAGTCC CATGTGTGAC AAGCACAACG CCTCTGTGGA AAAATCACAG 
GTGGGCTTCA TAGACTATAT TGTTCATCCA CTCTGGGAGA CATGGGCAGA CCTCGTACAT 
CCCGACGCCC AGGACATTTT GGACACTTTG GAGGACAATC GTGAGTGGTA CCAGAGCACA 
ATCCCCCAGA GCCCCTCCCC TGCACCTGAT GACCAAGAGG ACGGCCGTCA GGGACAGACT 
GAAAAATTCC AGTTCGAACT AACCTTAGAG GAAGATGGCG AGTCAGACAC TGAAAAGGAC 
AGTGGAAGTC AAGTGGAGGA AGACACTAGC TGCAGTGACT CTAAGACTCT GTGCACCCAA 
GACTCAGAGT CCACTGAAAT TCCCCTTGAC GAGCAGGTTG AAGAGGAGGC TGTAGCAGAA 
GAGGAAAGCC AGCCCCAAAC TGGCGTCGCA GACGATTGTT GTCCTGACAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( XM_008760723.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758945.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X3, mRNA.

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CloneID	ORa42935
Clone ID Related Accession (Same CDS sequence)	XM_008760723.1
Accession Version	XM_008760723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCA ACAAGTTCAA GAGGATGCTT AATCGGGAGC TCACCCACCT CTCTGAAATG 
AGTCGGTCTG GCAACCAGGT GTCGGAGTAC ATATCAAACA CGTTCTTAGA TAAGCAACAT 
GAAGTAGAAA TTCCCTCTCC GACTCAGAAG GAAAAAGAGA AGAAGAAAAG GCCGATGTCA 
CAGATCAGTG GGGTCAAGAA GTTGATGCAC AGCTCCAGCC TGACCAATTC CTGCATCCCA 
AGATTTGGGG TTAAAACAGA GCAGGAAGAT GTCCTGGCCA AGGAACTAGA AGACGTGAAC 
AAGTGGGGCC TCCACGTTTT CCGAATAGCG GAGCTGTCTG GCAACCGGCC TCTGACTGTT 
ATCATGCACA CCATTTTTCA GGAACGAGAT TTGTTAAAAA CGTTTAAAAT CCCAGTGGAC 
ACTTTGATTA CGTATCTTAT GACTCTAGAA GACCATTACC ATGCTGACGT GGCCTATCAC 
AACAACATCC ATGCTGCAGA TGTCGTCCAG TCAACTCATG TGCTGCTCTC TACACCCGCT 
TTGGAGGCTG TTTTCACTGA CTTGGAGATT CTCGCGGCCA TTTTTGCCAG TGCAATACAT 
GATGTGGATC ATCCTGGTGT GTCAAATCAA TTTCTGATCA ATACAAACTC GGAACTTGCC 
TTGATGTACA ACGACTCCTC CGTCTTAGAG AATCATCATT TGGCTGTGGG CTTTAAGTTG 
CTCCAGGAAG AAAACTGTGA CATTTTCCAG AATCTGACCA AAAAGCAAAG ACAATCTTTA 
AGGAAAATGG CCATTGACAT TGTACTAGCG ACAGACATGT CAAAGCACAT GAATCTGCTG 
GCTGATCTGA AAACAATGGT TGAAACGAAG AAGGTGACGA GCTCTGGCGT CCTCCTCCTT 
GATAACTATT CTGACAGGAT CCAGGTCCTC CAGAATATGG TGCACTGTGC AGACCTGAGC 
AACCCCACAA AGCCACTCCA GCTCTACCGC CAGTGGACGG ACCGGATAAT GGAGGAGTTC 
TTCCGTCAGG GGGACCGGGA GCGTGAGCGT GGCATGGAGA TAAGTCCCAT GTGTGACAAG 
CACAACGCCT CTGTGGAAAA ATCACAGGTG GGCTTCATAG ACTATATTGT TCATCCACTC 
TGGGAGACAT GGGCAGACCT CGTACATCCC GACGCCCAGG ACATTTTGGA CACTTTGGAG 
GACAATCGTG AGTGGTACCA GAGCACAATC CCCCAGAGCC CCTCCCCTGC ACCTGATGAC 
CAAGAGGACG GCCGTCAGGG ACAGACTGAA AAATTCCAGT TCGAACTAAC CTTAGAGGAA 
GATGGCGAGT CAGACACTGA AAAGGACAGT GGAAGTCAAG TGGAGGAAGA CACTAGCTGC 
AGTGACTCTA AGACTCTGTG CACCCAAGAC TCAGAGTCCA CTGAAATTCC CCTTGACGAG 
CAGGTTGAAG AGGAGGCTGT AGCAGAAGAG GAAAGCCAGC CCCAAACTGG CGTCGCAGAC 
GATTGTTGTC CTGACACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758945.1
CDS	243..1763
Translation	MASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	XM_008760723.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760723.1

ORF Insert Sequence:

ATGGCCTCCA ACAAGTTCAA GAGGATGCTT AATCGGGAGC TCACCCACCT CTCTGAAATG 
AGTCGGTCTG GCAACCAGGT GTCGGAGTAC ATATCAAACA CGTTCTTAGA TAAGCAACAT 
GAAGTAGAAA TTCCCTCTCC GACTCAGAAG GAAAAAGAGA AGAAGAAAAG GCCGATGTCA 
CAGATCAGTG GGGTCAAGAA GTTGATGCAC AGCTCCAGCC TGACCAATTC CTGCATCCCA 
AGATTTGGGG TTAAAACAGA GCAGGAAGAT GTCCTGGCCA AGGAACTAGA AGACGTGAAC 
AAGTGGGGCC TCCACGTTTT CCGAATAGCG GAGCTGTCTG GCAACCGGCC TCTGACTGTT 
ATCATGCACA CCATTTTTCA GGAACGAGAT TTGTTAAAAA CGTTTAAAAT CCCAGTGGAC 
ACTTTGATTA CGTATCTTAT GACTCTAGAA GACCATTACC ATGCTGACGT GGCCTATCAC 
AACAACATCC ATGCTGCAGA TGTCGTCCAG TCAACTCATG TGCTGCTCTC TACACCCGCT 
TTGGAGGCTG TTTTCACTGA CTTGGAGATT CTCGCGGCCA TTTTTGCCAG TGCAATACAT 
GATGTGGATC ATCCTGGTGT GTCAAATCAA TTTCTGATCA ATACAAACTC GGAACTTGCC 
TTGATGTACA ACGACTCCTC CGTCTTAGAG AATCATCATT TGGCTGTGGG CTTTAAGTTG 
CTCCAGGAAG AAAACTGTGA CATTTTCCAG AATCTGACCA AAAAGCAAAG ACAATCTTTA 
AGGAAAATGG CCATTGACAT TGTACTAGCG ACAGACATGT CAAAGCACAT GAATCTGCTG 
GCTGATCTGA AAACAATGGT TGAAACGAAG AAGGTGACGA GCTCTGGCGT CCTCCTCCTT 
GATAACTATT CTGACAGGAT CCAGGTCCTC CAGAATATGG TGCACTGTGC AGACCTGAGC 
AACCCCACAA AGCCACTCCA GCTCTACCGC CAGTGGACGG ACCGGATAAT GGAGGAGTTC 
TTCCGTCAGG GGGACCGGGA GCGTGAGCGT GGCATGGAGA TAAGTCCCAT GTGTGACAAG 
CACAACGCCT CTGTGGAAAA ATCACAGGTG GGCTTCATAG ACTATATTGT TCATCCACTC 
TGGGAGACAT GGGCAGACCT CGTACATCCC GACGCCCAGG ACATTTTGGA CACTTTGGAG 
GACAATCGTG AGTGGTACCA GAGCACAATC CCCCAGAGCC CCTCCCCTGC ACCTGATGAC 
CAAGAGGACG GCCGTCAGGG ACAGACTGAA AAATTCCAGT TCGAACTAAC CTTAGAGGAA 
GATGGCGAGT CAGACACTGA AAAGGACAGT GGAAGTCAAG TGGAGGAAGA CACTAGCTGC 
AGTGACTCTA AGACTCTGTG CACCCAAGAC TCAGAGTCCA CTGAAATTCC CCTTGACGAG 
CAGGTTGAAG AGGAGGCTGT AGCAGAAGAG GAAAGCCAGC CCCAAACTGG CGTCGCAGAC 
GATTGTTGTC CTGACACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( NM_001113329.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001106800.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 2, mRNA.

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CloneID	ORa00935
Clone ID Related Accession (Same CDS sequence)	NM_001113329.1
Accession Version	NM_001113329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF536979.1 and AF031373.1. Transcript Variant: This variant (2) has an alternate 5' coding sequence including two exons, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGGA ACACTTGCGA TGTACTTTCT CGGAGCAAAA GTGCCTCTGA GGAAACATTG 
CACTCCTGTA ATGATGAAGA GGACCCCTTC CGGGGAATGG AGCCCTATCT CGTACGGAGA 
CTTTCATCCC GCAGCATCCA GCTTCCTCCT CTTGCCTTCA GACAGTTGGA GCAAACGGAC 
TTGCGAAGCG AATCAGAGAA CATTCCGCGG CCAACTAGCC TCCCCTTGAA AATCCTGCCG 
CTGATTGCTG TCACATCCGC AGACTCGACT GGTTTCGATG TGGACAATGG CACATCAGCG 
GGACGGAGTC CCTTGGATCC CATGACCAGC CCAGGGTCTG GGCTAATTCT CCAAGCAAAC 
TTTGTGCACA GTCAACGCCG GGAGTCCTTC CTGTATCGAT CAGACAGCGA TTATGACCTC 
TCTCCAAAGT CTATGTCCAG GAACTCCTCG ATTGCCAGTG ATATACATGG AGATGACTTG 
ATTGTGACTC CATTTGCTCA GGTCTTGGCC AGCCTTCGAA CTGTAAGAAA CAACTTTGCT 
GCATTAACTA ATTTGCAAGA TCGCGCACCC AGCAAAAGAT CACCCATGTG CAACCAACCA 
TCCATCAACA AAGCCACCAT CACAGAGGAG GCCTACCAGA AACTGGCCAG CGAGACCCTG 
GAGGAGCTGG ACTGGTGTCT GGACCAGCTA GAGACCCTGC AGACCAGGCA CTCCGTCAGT 
GAGATGGCCT CCAACAAGTT CAAGAGGATG CTTAATCGGG AGCTCACCCA CCTCTCTGAA 
ATGAGTCGGT CTGGCAACCA GGTGTCGGAG TACATATCAA ACACGTTCTT AGATAAGCAA 
CATGAAGTAG AAATTCCCTC TCCGACTCAG AAGGAAAAAG AGAAGAAGAA AAGGCCGATG 
TCACAGATCA GTGGGGTCAA GAAGTTGATG CACAGCTCCA GCCTGACCAA TTCCTGCATT 
CCAAGATTTG GGGTTAAAAC AGAGCAGGAA GATGTCCTGG CCAAGGAACT AGAAGACGTG 
AACAAGTGGG GCCTCCACGT TTTCCGAATA GCGGAGCTGT CTGGCAACCG GCCTCTGACT 
GTTATCATGC ACACCATTTT TCAGGAACGA GATTTGTTAA AAACGTTTAA AATCCCAGTG 
GACACTTTGA TTACGTATCT TATGACTCTA GAAGACCATT ACCATGCTGA CGTGGCCTAT 
CACAACAACA TCCATGCTGC AGATGTCGTC CAGTCAACTC ATGTGCTGCT CTCTACACCC 
GCTTTGGAGG CTGTTTTCAC TGACTTGGAG ATTCTCGCGG CCATTTTTGC CAGTGCAATA 
CATGATGTGG ATCATCCTGG TGTGTCAAAT CAATTTCTGA TCAATACAAA CTCGGAACTT 
GCCTTGATGT ACAACGACTC CTCCGTCTTA GAGAATCATC ATTTGGCTGT GGGCTTTAAG 
TTGCTCCAGG AAGAAAACTG TGACATTTTC CAGAATCTGA CCAAAAAGCA AAGACAATCT 
TTAAGGAAAA TGGCCATTGA CATTGTACTA GCGACAGACA TGTCAAAGCA CATGAATCTG 
CTGGCTGATC TGAAAACAAT GGTTGAAACG AAGAAGGTGA CGAGCTCTGG CGTCCTCCTC 
CTTGATAACT ATTCTGACAG GATCCAGGTC CTCCAGAATA TGGTGCACTG TGCAGACCTG 
AGCAACCCCA CAAAGCCACT CCAGCTCTAC CGCCAGTGGA CGGACCGGAT AATGGAGGAG 
TTCTTCCGTC AGGGGGACCG GGAGCGTGAG CGTGGCATGG AGATAAGTCC CATGTGTGAC 
AAGCACAACG CCTCTGTGGA AAAATCACAG GTGGGCTTCA TAGACTACAT TGTTCATCCA 
CTCTGGGAGA CATGGGCAGA CCTCGTACAT CCCGACGCCC AGGACATTTT GGACACTTTG 
GAGGACAATC GTGAGTGGTA CCAGAGCACA ATCCCCCAGA GCCCCTCCCC TGCACCTGAT 
GACCAAGAGG ACGGCCGTCA GGGACAGACT GAAAAATTCC AGTTCGAACT AACCTTAGAG 
GAAGATGGCG AGTCAGACAC TGAAAAGGAC AGTGGAAGTC AAGTGGAGGA AGACACTAGC 
TGCAGTGACT CTAAGACTCT GTGCACCCAA GACTCAGAGT CCACTGAAAT TCCCCTTGAC 
GAGCAGGTTG AAGAGGAGGC TGTAGCAGAA GAGGAAAGCC AGCCCCAAAC TGGCGTCGCA 
GACGATTGTT GTCCTGACAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106800.1
CDS	84..2327
Translation	MERNTCDVLSRSKSASEETLHSCNDEEDPFRGMEPYLVRRLSSRSIQLPPLAFRQLEQTDLRSESENIPRPTSLPLKILPLIAVTSADSTGFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	NM_001113329.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113329.1

ORF Insert Sequence:

ATGGAGAGGA ACACTTGCGA TGTACTTTCT CGGAGCAAAA GTGCCTCTGA GGAAACATTG 
CACTCCTGTA ATGATGAAGA GGACCCCTTC CGGGGAATGG AGCCCTATCT CGTACGGAGA 
CTTTCATCCC GCAGCATCCA GCTTCCTCCT CTTGCCTTCA GACAGTTGGA GCAAACGGAC 
TTGCGAAGCG AATCAGAGAA CATTCCGCGG CCAACTAGCC TCCCCTTGAA AATCCTGCCG 
CTGATTGCTG TCACATCCGC AGACTCGACT GGTTTCGATG TGGACAATGG CACATCAGCG 
GGACGGAGTC CCTTGGATCC CATGACCAGC CCAGGGTCTG GGCTAATTCT CCAAGCAAAC 
TTTGTGCACA GTCAACGCCG GGAGTCCTTC CTGTATCGAT CAGACAGCGA TTATGACCTC 
TCTCCAAAGT CTATGTCCAG GAACTCCTCG ATTGCCAGTG ATATACATGG AGATGACTTG 
ATTGTGACTC CATTTGCTCA GGTCTTGGCC AGCCTTCGAA CTGTAAGAAA CAACTTTGCT 
GCATTAACTA ATTTGCAAGA TCGCGCACCC AGCAAAAGAT CACCCATGTG CAACCAACCA 
TCCATCAACA AAGCCACCAT CACAGAGGAG GCCTACCAGA AACTGGCCAG CGAGACCCTG 
GAGGAGCTGG ACTGGTGTCT GGACCAGCTA GAGACCCTGC AGACCAGGCA CTCCGTCAGT 
GAGATGGCCT CCAACAAGTT CAAGAGGATG CTTAATCGGG AGCTCACCCA CCTCTCTGAA 
ATGAGTCGGT CTGGCAACCA GGTGTCGGAG TACATATCAA ACACGTTCTT AGATAAGCAA 
CATGAAGTAG AAATTCCCTC TCCGACTCAG AAGGAAAAAG AGAAGAAGAA AAGGCCGATG 
TCACAGATCA GTGGGGTCAA GAAGTTGATG CACAGCTCCA GCCTGACCAA TTCCTGCATT 
CCAAGATTTG GGGTTAAAAC AGAGCAGGAA GATGTCCTGG CCAAGGAACT AGAAGACGTG 
AACAAGTGGG GCCTCCACGT TTTCCGAATA GCGGAGCTGT CTGGCAACCG GCCTCTGACT 
GTTATCATGC ACACCATTTT TCAGGAACGA GATTTGTTAA AAACGTTTAA AATCCCAGTG 
GACACTTTGA TTACGTATCT TATGACTCTA GAAGACCATT ACCATGCTGA CGTGGCCTAT 
CACAACAACA TCCATGCTGC AGATGTCGTC CAGTCAACTC ATGTGCTGCT CTCTACACCC 
GCTTTGGAGG CTGTTTTCAC TGACTTGGAG ATTCTCGCGG CCATTTTTGC CAGTGCAATA 
CATGATGTGG ATCATCCTGG TGTGTCAAAT CAATTTCTGA TCAATACAAA CTCGGAACTT 
GCCTTGATGT ACAACGACTC CTCCGTCTTA GAGAATCATC ATTTGGCTGT GGGCTTTAAG 
TTGCTCCAGG AAGAAAACTG TGACATTTTC CAGAATCTGA CCAAAAAGCA AAGACAATCT 
TTAAGGAAAA TGGCCATTGA CATTGTACTA GCGACAGACA TGTCAAAGCA CATGAATCTG 
CTGGCTGATC TGAAAACAAT GGTTGAAACG AAGAAGGTGA CGAGCTCTGG CGTCCTCCTC 
CTTGATAACT ATTCTGACAG GATCCAGGTC CTCCAGAATA TGGTGCACTG TGCAGACCTG 
AGCAACCCCA CAAAGCCACT CCAGCTCTAC CGCCAGTGGA CGGACCGGAT AATGGAGGAG 
TTCTTCCGTC AGGGGGACCG GGAGCGTGAG CGTGGCATGG AGATAAGTCC CATGTGTGAC 
AAGCACAACG CCTCTGTGGA AAAATCACAG GTGGGCTTCA TAGACTACAT TGTTCATCCA 
CTCTGGGAGA CATGGGCAGA CCTCGTACAT CCCGACGCCC AGGACATTTT GGACACTTTG 
GAGGACAATC GTGAGTGGTA CCAGAGCACA ATCCCCCAGA GCCCCTCCCC TGCACCTGAT 
GACCAAGAGG ACGGCCGTCA GGGACAGACT GAAAAATTCC AGTTCGAACT AACCTTAGAG 
GAAGATGGCG AGTCAGACAC TGAAAAGGAC AGTGGAAGTC AAGTGGAGGA AGACACTAGC 
TGCAGTGACT CTAAGACTCT GTGCACCCAA GACTCAGAGT CCACTGAAAT TCCCCTTGAC 
GAGCAGGTTG AAGAGGAGGC TGTAGCAGAA GAGGAAAGCC AGCCCCAAAC TGGCGTCGCA 
GACGATTGTT GTCCTGACAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( NM_001113334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001106805.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 4, mRNA.

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CloneID	ORa00940
Clone ID Related Accession (Same CDS sequence)	NM_001113334.1
Accession Version	NM_001113334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EF121818.1 and AF031373.1. Transcript Variant: This variant (4) has an alternate 5' coding exon, as compared to variant 1. The resulting isoform (4) has a shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF121818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTCG TTTGGGATCC TCTGGGAGTC ACAGTGCCAG GTCCATCTCC AAGAACTAGG 
ACAAGATTGC GTTTCTCAAA GTCCTACAGT TTCGATGTGG ACAATGGCAC ATCAGCGGGA 
CGGAGTCCCT TGGATCCCAT GACCAGCCCA GGGTCTGGGC TAATTCTCCA AGCAAACTTT 
GTGCACAGTC AACGCCGGGA GTCCTTCCTG TATCGATCAG ACAGCGATTA TGACCTCTCT 
CCAAAGTCTA TGTCCAGGAA CTCCTCGATT GCCAGTGATA TACATGGAGA TGACTTGATT 
GTGACTCCAT TTGCTCAGGT CTTGGCCAGC CTTCGAACTG TAAGAAACAA CTTTGCTGCA 
TTAACTAATT TGCAAGATCG CGCACCCAGC AAAAGATCAC CCATGTGCAA CCAACCATCC 
ATCAACAAAG CCACCATCAC AGAGGAGGCC TACCAGAAAC TGGCCAGCGA GACCCTGGAG 
GAGCTGGACT GGTGTCTGGA CCAGCTAGAG ACCCTGCAGA CCAGGCACTC CGTCAGTGAG 
ATGGCCTCCA ACAAGTTCAA GAGGATGCTT AATCGGGAGC TCACCCACCT CTCTGAAATG 
AGTCGGTCTG GCAACCAGGT GTCGGAGTAC ATATCAAACA CGTTCTTAGA TAAGCAACAT 
GAAGTAGAAA TTCCCTCTCC GACTCAGAAG GAAAAAGAGA AGAAGAAAAG GCCGATGTCA 
CAGATCAGTG GGGTCAAGAA GTTGATGCAC AGCTCCAGCC TGACCAATTC CTGCATTCCA 
AGATTTGGGG TTAAAACAGA GCAGGAAGAT GTCCTGGCCA AGGAACTAGA AGACGTGAAC 
AAGTGGGGCC TCCACGTTTT CCGAATAGCG GAGCTGTCTG GCAACCGGCC TCTGACTGTT 
ATCATGCACA CCATTTTTCA GGAACGAGAT TTGTTAAAAA CGTTTAAAAT CCCAGTGGAC 
ACTTTGATTA CGTATCTTAT GACTCTAGAA GACCATTACC ATGCTGACGT GGCCTATCAC 
AACAACATCC ATGCTGCAGA TGTCGTCCAG TCAACTCATG TGCTGCTCTC TACACCCGCT 
TTGGAGGCTG TTTTCACTGA CTTGGAGATT CTCGCGGCCA TTTTTGCCAG TGCAATACAT 
GATGTGGATC ATCCTGGTGT GTCAAATCAA TTTCTGATCA ATACAAACTC GGAACTTGCC 
TTGATGTACA ACGACTCCTC CGTCTTAGAG AATCATCATT TGGCTGTGGG CTTTAAGTTG 
CTCCAGGAAG AAAACTGTGA CATTTTCCAG AATCTGACCA AAAAGCAAAG ACAATCTTTA 
AGGAAAATGG CCATTGACAT TGTACTAGCG ACAGACATGT CAAAGCACAT GAATCTGCTG 
GCTGATCTGA AAACAATGGT TGAAACGAAG AAGGTGACGA GCTCTGGCGT CCTCCTCCTT 
GATAACTATT CTGACAGGAT CCAGGTCCTC CAGAATATGG TGCACTGTGC AGACCTGAGC 
AACCCCACAA AGCCACTCCA GCTCTACCGC CAGTGGACGG ACCGGATAAT GGAGGAGTTC 
TTCCGTCAGG GGGACCGGGA GCGTGAGCGT GGCATGGAGA TAAGTCCCAT GTGTGACAAG 
CACAACGCCT CTGTGGAAAA ATCACAGGTG GGCTTCATAG ACTACATTGT TCATCCACTC 
TGGGAGACAT GGGCAGACCT CGTACATCCC GACGCCCAGG ACATTTTGGA CACTTTGGAG 
GACAATCGTG AGTGGTACCA GAGCACAATC CCCCAGAGCC CCTCCCCTGC ACCTGATGAC 
CAAGAGGACG GCCGTCAGGG ACAGACTGAA AAATTCCAGT TCGAACTAAC CTTAGAGGAA 
GATGGCGAGT CAGACACTGA AAAGGACAGT GGAAGTCAAG TGGAGGAAGA CACTAGCTGC 
AGTGACTCTA AGACTCTGTG CACCCAAGAC TCAGAGTCCA CTGAAATTCC CCTTGACGAG 
CAGGTTGAAG AGGAGGCTGT AGCAGAAGAG GAAAGCCAGC CCCAAACTGG CGTCGCAGAC 
GATTGTTGTC CTGACACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106805.1
CDS	41..2101
Translation	MAFVWDPLGVTVPGPSPRTRTRLRFSKSYSFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	NM_001113334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113334.1

ORF Insert Sequence:

ATGGCTTTCG TTTGGGATCC TCTGGGAGTC ACAGTGCCAG GTCCATCTCC AAGAACTAGG 
ACAAGATTGC GTTTCTCAAA GTCCTACAGT TTCGATGTGG ACAATGGCAC ATCAGCGGGA 
CGGAGTCCCT TGGATCCCAT GACCAGCCCA GGGTCTGGGC TAATTCTCCA AGCAAACTTT 
GTGCACAGTC AACGCCGGGA GTCCTTCCTG TATCGATCAG ACAGCGATTA TGACCTCTCT 
CCAAAGTCTA TGTCCAGGAA CTCCTCGATT GCCAGTGATA TACATGGAGA TGACTTGATT 
GTGACTCCAT TTGCTCAGGT CTTGGCCAGC CTTCGAACTG TAAGAAACAA CTTTGCTGCA 
TTAACTAATT TGCAAGATCG CGCACCCAGC AAAAGATCAC CCATGTGCAA CCAACCATCC 
ATCAACAAAG CCACCATCAC AGAGGAGGCC TACCAGAAAC TGGCCAGCGA GACCCTGGAG 
GAGCTGGACT GGTGTCTGGA CCAGCTAGAG ACCCTGCAGA CCAGGCACTC CGTCAGTGAG 
ATGGCCTCCA ACAAGTTCAA GAGGATGCTT AATCGGGAGC TCACCCACCT CTCTGAAATG 
AGTCGGTCTG GCAACCAGGT GTCGGAGTAC ATATCAAACA CGTTCTTAGA TAAGCAACAT 
GAAGTAGAAA TTCCCTCTCC GACTCAGAAG GAAAAAGAGA AGAAGAAAAG GCCGATGTCA 
CAGATCAGTG GGGTCAAGAA GTTGATGCAC AGCTCCAGCC TGACCAATTC CTGCATTCCA 
AGATTTGGGG TTAAAACAGA GCAGGAAGAT GTCCTGGCCA AGGAACTAGA AGACGTGAAC 
AAGTGGGGCC TCCACGTTTT CCGAATAGCG GAGCTGTCTG GCAACCGGCC TCTGACTGTT 
ATCATGCACA CCATTTTTCA GGAACGAGAT TTGTTAAAAA CGTTTAAAAT CCCAGTGGAC 
ACTTTGATTA CGTATCTTAT GACTCTAGAA GACCATTACC ATGCTGACGT GGCCTATCAC 
AACAACATCC ATGCTGCAGA TGTCGTCCAG TCAACTCATG TGCTGCTCTC TACACCCGCT 
TTGGAGGCTG TTTTCACTGA CTTGGAGATT CTCGCGGCCA TTTTTGCCAG TGCAATACAT 
GATGTGGATC ATCCTGGTGT GTCAAATCAA TTTCTGATCA ATACAAACTC GGAACTTGCC 
TTGATGTACA ACGACTCCTC CGTCTTAGAG AATCATCATT TGGCTGTGGG CTTTAAGTTG 
CTCCAGGAAG AAAACTGTGA CATTTTCCAG AATCTGACCA AAAAGCAAAG ACAATCTTTA 
AGGAAAATGG CCATTGACAT TGTACTAGCG ACAGACATGT CAAAGCACAT GAATCTGCTG 
GCTGATCTGA AAACAATGGT TGAAACGAAG AAGGTGACGA GCTCTGGCGT CCTCCTCCTT 
GATAACTATT CTGACAGGAT CCAGGTCCTC CAGAATATGG TGCACTGTGC AGACCTGAGC 
AACCCCACAA AGCCACTCCA GCTCTACCGC CAGTGGACGG ACCGGATAAT GGAGGAGTTC 
TTCCGTCAGG GGGACCGGGA GCGTGAGCGT GGCATGGAGA TAAGTCCCAT GTGTGACAAG 
CACAACGCCT CTGTGGAAAA ATCACAGGTG GGCTTCATAG ACTACATTGT TCATCCACTC 
TGGGAGACAT GGGCAGACCT CGTACATCCC GACGCCCAGG ACATTTTGGA CACTTTGGAG 
GACAATCGTG AGTGGTACCA GAGCACAATC CCCCAGAGCC CCTCCCCTGC ACCTGATGAC 
CAAGAGGACG GCCGTCAGGG ACAGACTGAA AAATTCCAGT TCGAACTAAC CTTAGAGGAA 
GATGGCGAGT CAGACACTGA AAAGGACAGT GGAAGTCAAG TGGAGGAAGA CACTAGCTGC 
AGTGACTCTA AGACTCTGTG CACCCAAGAC TCAGAGTCCA CTGAAATTCC CCTTGACGAG 
CAGGTTGAAG AGGAGGCTGT AGCAGAAGAG GAAAGCCAGC CCCAAACTGG CGTCGCAGAC 
GATTGTTGTC CTGACACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( NM_001113328.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001106799.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa39888
Clone ID Related Accession (Same CDS sequence)	NM_001113328.1
Accession Version	NM_001113328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07008053.1 and AF031373.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF031373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCAG AGGGCAGCAG CGTGCCGGCC CGGGCGGGCA GCGACGAGGG CAGCGACAGC 
TCTGGAGGGG CCGCGCTCAA AGCCCCCAAG CATCTCTGGA GGCACGAGCA GCACCACCAG 
TACCCGCTCC GGCAGCCCCA GTTCCGCCTC TTGCACCCCC ATCACCACCT GCCCCCGCCG 
CCGCCACCCT CGCCCCAGCC CCAGCTGCAG CCACCACCAC CGCCCCCACT GCCACCACCC 
CCGCCACCGC CCGGAGCTAC CCGTGGTCGC TACGCCTCAA GCGGGGCTAG CCGCGTCCGG 
CACCGCGGCT ACTCGGACAC GGAGCGCTAC CTGTACTGCC GTGCCATGGA CCGCACCTCC 
TACGCGGTGG AGACTGGTCA CCGGCCAGGC CTGAAGAAAT CTAGGATGTC CTGGCCCTCG 
TCTTTCCAGG GACTCAGACG TTTCGATGTG GACAATGGCA CATCAGCGGG ACGGAGTCCC 
TTGGATCCCA TGACCAGCCC AGGGTCTGGG CTAATTCTCC AAGCAAACTT TGTGCACAGT 
CAACGCCGGG AGTCCTTCCT GTATCGATCA GACAGCGATT ATGACCTCTC TCCAAAGTCT 
ATGTCCAGGA ACTCCTCGAT TGCCAGTGAT ATACATGGAG ATGACTTGAT TGTGACTCCA 
TTTGCTCAGG TCTTGGCCAG CCTTCGAACT GTAAGAAACA ACTTTGCTGC ATTAACTAAT 
TTGCAAGATC GCGCACCCAG CAAAAGATCA CCCATGTGCA ACCAACCATC CATCAACAAA 
GCCACCATCA CAGAGGAGGC CTACCAGAAA CTGGCCAGCG AGACCCTGGA GGAGCTGGAC 
TGGTGTCTGG ACCAGCTAGA GACCCTGCAG ACCAGGCACT CCGTCAGTGA GATGGCCTCC 
AACAAGTTCA AGAGGATGCT TAATCGGGAG CTCACCCACC TCTCTGAAAT GAGTCGGTCT 
GGCAACCAGG TGTCGGAGTA CATATCAAAC ACGTTCTTAG ATAAGCAACA TGAAGTAGAA 
ATTCCCTCTC CGACTCAGAA GGAAAAAGAG AAGAAGAAAA GGCCGATGTC ACAGATCAGT 
GGGGTCAAGA AGTTGATGCA CAGCTCCAGC CTGACCAATT CCTGCATTCC AAGATTTGGG 
GTTAAAACAG AGCAGGAAGA TGTCCTGGCC AAGGAACTAG AAGACGTGAA CAAGTGGGGC 
CTCCACGTTT TCCGAATAGC GGAGCTGTCT GGCAACCGGC CTCTGACTGT TATCATGCAC 
ACCATTTTTC AGGAACGAGA TTTGTTAAAA ACGTTTAAAA TCCCAGTGGA CACTTTGATT 
ACGTATCTTA TGACTCTAGA AGACCATTAC CATGCTGACG TGGCCTATCA CAACAACATC 
CATGCTGCAG ATGTCGTCCA GTCAACTCAT GTGCTGCTCT CTACACCCGC TTTGGAGGCT 
GTTTTCACTG ACTTGGAGAT TCTCGCGGCC ATTTTTGCCA GTGCAATACA TGATGTGGAT 
CATCCTGGTG TGTCAAATCA ATTTCTGATC AATACAAACT CGGAACTTGC CTTGATGTAC 
AACGACTCCT CCGTCTTAGA GAATCATCAT TTGGCTGTGG GCTTTAAGTT GCTCCAGGAA 
GAAAACTGTG ACATTTTCCA GAATCTGACC AAAAAGCAAA GACAATCTTT AAGGAAAATG 
GCCATTGACA TTGTACTAGC GACAGACATG TCAAAGCACA TGAATCTGCT GGCTGATCTG 
AAAACAATGG TTGAAACGAA GAAGGTGACG AGCTCTGGCG TCCTCCTCCT TGATAACTAT 
TCTGACAGGA TCCAGGTCCT CCAGAATATG GTGCACTGTG CAGACCTGAG CAACCCCACA 
AAGCCACTCC AGCTCTACCG CCAGTGGACG GACCGGATAA TGGAGGAGTT CTTCCGTCAG 
GGGGACCGGG AGCGTGAGCG TGGCATGGAG ATAAGTCCCA TGTGTGACAA GCACAACGCC 
TCTGTGGAAA AATCACAGGT GGGCTTCATA GACTACATTG TTCATCCACT CTGGGAGACA 
TGGGCAGACC TCGTACATCC CGACGCCCAG GACATTTTGG ACACTTTGGA GGACAATCGT 
GAGTGGTACC AGAGCACAAT CCCCCAGAGC CCCTCCCCTG CACCTGATGA CCAAGAGGAC 
GGCCGTCAGG GACAGACTGA AAAATTCCAG TTCGAACTAA CCTTAGAGGA AGATGGCGAG 
TCAGACACTG AAAAGGACAG TGGAAGTCAA GTGGAGGAAG ACACTAGCTG CAGTGACTCT 
AAGACTCTGT GCACCCAAGA CTCAGAGTCC ACTGAAATTC CCCTTGACGA GCAGGTTGAA 
GAGGAGGCTG TAGCAGAAGA GGAAAGCCAG CCCCAAACTG GCGTCGCAGA CGATTGTTGT 
CCTGACACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106799.1
CDS	1..2412
Translation	MEAEGSSVPARAGSDEGSDSSGGAALKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQLQPPPPPPLPPPPPPPGATRGRYASSGASRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	NM_001113328.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113328.1

ORF Insert Sequence:

ATGGAGGCAG AGGGCAGCAG CGTGCCGGCC CGGGCGGGCA GCGACGAGGG CAGCGACAGC 
TCTGGAGGGG CCGCGCTCAA AGCCCCCAAG CATCTCTGGA GGCACGAGCA GCACCACCAG 
TACCCGCTCC GGCAGCCCCA GTTCCGCCTC TTGCACCCCC ATCACCACCT GCCCCCGCCG 
CCGCCACCCT CGCCCCAGCC CCAGCTGCAG CCACCACCAC CGCCCCCACT GCCACCACCC 
CCGCCACCGC CCGGAGCTAC CCGTGGTCGC TACGCCTCAA GCGGGGCTAG CCGCGTCCGG 
CACCGCGGCT ACTCGGACAC GGAGCGCTAC CTGTACTGCC GTGCCATGGA CCGCACCTCC 
TACGCGGTGG AGACTGGTCA CCGGCCAGGC CTGAAGAAAT CTAGGATGTC CTGGCCCTCG 
TCTTTCCAGG GACTCAGACG TTTCGATGTG GACAATGGCA CATCAGCGGG ACGGAGTCCC 
TTGGATCCCA TGACCAGCCC AGGGTCTGGG CTAATTCTCC AAGCAAACTT TGTGCACAGT 
CAACGCCGGG AGTCCTTCCT GTATCGATCA GACAGCGATT ATGACCTCTC TCCAAAGTCT 
ATGTCCAGGA ACTCCTCGAT TGCCAGTGAT ATACATGGAG ATGACTTGAT TGTGACTCCA 
TTTGCTCAGG TCTTGGCCAG CCTTCGAACT GTAAGAAACA ACTTTGCTGC ATTAACTAAT 
TTGCAAGATC GCGCACCCAG CAAAAGATCA CCCATGTGCA ACCAACCATC CATCAACAAA 
GCCACCATCA CAGAGGAGGC CTACCAGAAA CTGGCCAGCG AGACCCTGGA GGAGCTGGAC 
TGGTGTCTGG ACCAGCTAGA GACCCTGCAG ACCAGGCACT CCGTCAGTGA GATGGCCTCC 
AACAAGTTCA AGAGGATGCT TAATCGGGAG CTCACCCACC TCTCTGAAAT GAGTCGGTCT 
GGCAACCAGG TGTCGGAGTA CATATCAAAC ACGTTCTTAG ATAAGCAACA TGAAGTAGAA 
ATTCCCTCTC CGACTCAGAA GGAAAAAGAG AAGAAGAAAA GGCCGATGTC ACAGATCAGT 
GGGGTCAAGA AGTTGATGCA CAGCTCCAGC CTGACCAATT CCTGCATTCC AAGATTTGGG 
GTTAAAACAG AGCAGGAAGA TGTCCTGGCC AAGGAACTAG AAGACGTGAA CAAGTGGGGC 
CTCCACGTTT TCCGAATAGC GGAGCTGTCT GGCAACCGGC CTCTGACTGT TATCATGCAC 
ACCATTTTTC AGGAACGAGA TTTGTTAAAA ACGTTTAAAA TCCCAGTGGA CACTTTGATT 
ACGTATCTTA TGACTCTAGA AGACCATTAC CATGCTGACG TGGCCTATCA CAACAACATC 
CATGCTGCAG ATGTCGTCCA GTCAACTCAT GTGCTGCTCT CTACACCCGC TTTGGAGGCT 
GTTTTCACTG ACTTGGAGAT TCTCGCGGCC ATTTTTGCCA GTGCAATACA TGATGTGGAT 
CATCCTGGTG TGTCAAATCA ATTTCTGATC AATACAAACT CGGAACTTGC CTTGATGTAC 
AACGACTCCT CCGTCTTAGA GAATCATCAT TTGGCTGTGG GCTTTAAGTT GCTCCAGGAA 
GAAAACTGTG ACATTTTCCA GAATCTGACC AAAAAGCAAA GACAATCTTT AAGGAAAATG 
GCCATTGACA TTGTACTAGC GACAGACATG TCAAAGCACA TGAATCTGCT GGCTGATCTG 
AAAACAATGG TTGAAACGAA GAAGGTGACG AGCTCTGGCG TCCTCCTCCT TGATAACTAT 
TCTGACAGGA TCCAGGTCCT CCAGAATATG GTGCACTGTG CAGACCTGAG CAACCCCACA 
AAGCCACTCC AGCTCTACCG CCAGTGGACG GACCGGATAA TGGAGGAGTT CTTCCGTCAG 
GGGGACCGGG AGCGTGAGCG TGGCATGGAG ATAAGTCCCA TGTGTGACAA GCACAACGCC 
TCTGTGGAAA AATCACAGGT GGGCTTCATA GACTACATTG TTCATCCACT CTGGGAGACA 
TGGGCAGACC TCGTACATCC CGACGCCCAG GACATTTTGG ACACTTTGGA GGACAATCGT 
GAGTGGTACC AGAGCACAAT CCCCCAGAGC CCCTCCCCTG CACCTGATGA CCAAGAGGAC 
GGCCGTCAGG GACAGACTGA AAAATTCCAG TTCGAACTAA CCTTAGAGGA AGATGGCGAG 
TCAGACACTG AAAAGGACAG TGGAAGTCAA GTGGAGGAAG ACACTAGCTG CAGTGACTCT 
AAGACTCTGT GCACCCAAGA CTCAGAGTCC ACTGAAATTC CCCTTGACGA GCAGGTTGAA 
GAGGAGGCTG TAGCAGAAGA GGAAAGCCAG CCCCAAACTG GCGTCGCAGA CGATTGTTGT 
CCTGACACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( NM_001113332.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001106803.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa42757
Clone ID Related Accession (Same CDS sequence)	NM_001113332.1
Accession Version	NM_001113332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EF102484.1 and AF031373.1. Transcript Variant: This variant (3) has an alternate 5' coding exon, as compared to variant 1. The resulting isoform (3) has a shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF102484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGC AGACGACAAG CCCGGACACC CTTACAGTCC CTGAAGTGGA TAATCCGCAT 
GTTCCAAACC CATGGCTCAA TGAGGATCTT GTGAAATCCC TGCGGGAAAA CCTGTTGCAG 
CACGAAAAGT CAAAGACAGC GAGGAAATCC GTTTCTCCCA AGCTCTCCCC GGTGATTTCT 
CCCAGGAATT CCCCCAGGCT CCTGCGCAGG ATGCTTCTGA GTAGCAACAT CCCCAAGCAG 
CGTCGCTTCA CAGTGGCACA TACATGTTTC GATGTGGACA ATGGCACATC AGCGGGACGG 
AGTCCCTTGG ATCCCATGAC CAGCCCAGGG TCTGGGCTAA TTCTCCAAGC AAACTTTGTG 
CACAGTCAAC GCCGGGAGTC CTTCCTGTAT CGATCAGACA GCGATTATGA CCTCTCTCCA 
AAGTCTATGT CCAGGAACTC CTCGATTGCC AGTGATATAC ATGGAGATGA CTTGATTGTG 
ACTCCATTTG CTCAGGTCTT GGCCAGCCTT CGAACTGTAA GAAACAACTT TGCTGCATTA 
ACTAATTTGC AAGATCGCGC ACCCAGCAAA AGATCACCCA TGTGCAACCA ACCATCCATC 
AACAAAGCCA CCATCACAGA GGAGGCCTAC CAGAAACTGG CCAGCGAGAC CCTGGAGGAG 
CTGGACTGGT GTCTGGACCA GCTAGAGACC CTGCAGACCA GGCACTCCGT CAGTGAGATG 
GCCTCCAACA AGTTCAAGAG GATGCTTAAT CGGGAGCTCA CCCACCTCTC TGAAATGAGT 
CGGTCTGGCA ACCAGGTGTC GGAGTACATA TCAAACACGT TCTTAGATAA GCAACATGAA 
GTAGAAATTC CCTCTCCGAC TCAGAAGGAA AAAGAGAAGA AGAAAAGGCC GATGTCACAG 
ATCAGTGGGG TCAAGAAGTT GATGCACAGC TCCAGCCTGA CCAATTCCTG CATTCCAAGA 
TTTGGGGTTA AAACAGAGCA GGAAGATGTC CTGGCCAAGG AACTAGAAGA CGTGAACAAG 
TGGGGCCTCC ACGTTTTCCG AATAGCGGAG CTGTCTGGCA ACCGGCCTCT GACTGTTATC 
ATGCACACCA TTTTTCAGGA ACGAGATTTG TTAAAAACGT TTAAAATCCC AGTGGACACT 
TTGATTACGT ATCTTATGAC TCTAGAAGAC CATTACCATG CTGACGTGGC CTATCACAAC 
AACATCCATG CTGCAGATGT CGTCCAGTCA ACTCATGTGC TGCTCTCTAC ACCCGCTTTG 
GAGGCTGTTT TCACTGACTT GGAGATTCTC GCGGCCATTT TTGCCAGTGC AATACATGAT 
GTGGATCATC CTGGTGTGTC AAATCAATTT CTGATCAATA CAAACTCGGA ACTTGCCTTG 
ATGTACAACG ACTCCTCCGT CTTAGAGAAT CATCATTTGG CTGTGGGCTT TAAGTTGCTC 
CAGGAAGAAA ACTGTGACAT TTTCCAGAAT CTGACCAAAA AGCAAAGACA ATCTTTAAGG 
AAAATGGCCA TTGACATTGT ACTAGCGACA GACATGTCAA AGCACATGAA TCTGCTGGCT 
GATCTGAAAA CAATGGTTGA AACGAAGAAG GTGACGAGCT CTGGCGTCCT CCTCCTTGAT 
AACTATTCTG ACAGGATCCA GGTCCTCCAG AATATGGTGC ACTGTGCAGA CCTGAGCAAC 
CCCACAAAGC CACTCCAGCT CTACCGCCAG TGGACGGACC GGATAATGGA GGAGTTCTTC 
CGTCAGGGGG ACCGGGAGCG TGAGCGTGGC ATGGAGATAA GTCCCATGTG TGACAAGCAC 
AACGCCTCTG TGGAAAAATC ACAGGTGGGC TTCATAGACT ACATTGTTCA TCCACTCTGG 
GAGACATGGG CAGACCTCGT ACATCCCGAC GCCCAGGACA TTTTGGACAC TTTGGAGGAC 
AATCGTGAGT GGTACCAGAG CACAATCCCC CAGAGCCCCT CCCCTGCACC TGATGACCAA 
GAGGACGGCC GTCAGGGACA GACTGAAAAA TTCCAGTTCG AACTAACCTT AGAGGAAGAT 
GGCGAGTCAG ACACTGAAAA GGACAGTGGA AGTCAAGTGG AGGAAGACAC TAGCTGCAGT 
GACTCTAAGA CTCTGTGCAC CCAAGACTCA GAGTCCACTG AAATTCCCCT TGACGAGCAG 
GTTGAAGAGG AGGCTGTAGC AGAAGAGGAA AGCCAGCCCC AAACTGGCGT CGCAGACGAT 
TGTTGTCCTG ACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106803.1
CDS	27..2264
Translation	MAQQTTSPDTLTVPEVDNPHVPNPWLNEDLVKSLRENLLQHEKSKTARKSVSPKLSPVISPRNSPRLLRRMLLSSNIPKQRRFTVAHTCFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	NM_001113332.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113332.1

ORF Insert Sequence:

ATGGCTCAGC AGACGACAAG CCCGGACACC CTTACAGTCC CTGAAGTGGA TAATCCGCAT 
GTTCCAAACC CATGGCTCAA TGAGGATCTT GTGAAATCCC TGCGGGAAAA CCTGTTGCAG 
CACGAAAAGT CAAAGACAGC GAGGAAATCC GTTTCTCCCA AGCTCTCCCC GGTGATTTCT 
CCCAGGAATT CCCCCAGGCT CCTGCGCAGG ATGCTTCTGA GTAGCAACAT CCCCAAGCAG 
CGTCGCTTCA CAGTGGCACA TACATGTTTC GATGTGGACA ATGGCACATC AGCGGGACGG 
AGTCCCTTGG ATCCCATGAC CAGCCCAGGG TCTGGGCTAA TTCTCCAAGC AAACTTTGTG 
CACAGTCAAC GCCGGGAGTC CTTCCTGTAT CGATCAGACA GCGATTATGA CCTCTCTCCA 
AAGTCTATGT CCAGGAACTC CTCGATTGCC AGTGATATAC ATGGAGATGA CTTGATTGTG 
ACTCCATTTG CTCAGGTCTT GGCCAGCCTT CGAACTGTAA GAAACAACTT TGCTGCATTA 
ACTAATTTGC AAGATCGCGC ACCCAGCAAA AGATCACCCA TGTGCAACCA ACCATCCATC 
AACAAAGCCA CCATCACAGA GGAGGCCTAC CAGAAACTGG CCAGCGAGAC CCTGGAGGAG 
CTGGACTGGT GTCTGGACCA GCTAGAGACC CTGCAGACCA GGCACTCCGT CAGTGAGATG 
GCCTCCAACA AGTTCAAGAG GATGCTTAAT CGGGAGCTCA CCCACCTCTC TGAAATGAGT 
CGGTCTGGCA ACCAGGTGTC GGAGTACATA TCAAACACGT TCTTAGATAA GCAACATGAA 
GTAGAAATTC CCTCTCCGAC TCAGAAGGAA AAAGAGAAGA AGAAAAGGCC GATGTCACAG 
ATCAGTGGGG TCAAGAAGTT GATGCACAGC TCCAGCCTGA CCAATTCCTG CATTCCAAGA 
TTTGGGGTTA AAACAGAGCA GGAAGATGTC CTGGCCAAGG AACTAGAAGA CGTGAACAAG 
TGGGGCCTCC ACGTTTTCCG AATAGCGGAG CTGTCTGGCA ACCGGCCTCT GACTGTTATC 
ATGCACACCA TTTTTCAGGA ACGAGATTTG TTAAAAACGT TTAAAATCCC AGTGGACACT 
TTGATTACGT ATCTTATGAC TCTAGAAGAC CATTACCATG CTGACGTGGC CTATCACAAC 
AACATCCATG CTGCAGATGT CGTCCAGTCA ACTCATGTGC TGCTCTCTAC ACCCGCTTTG 
GAGGCTGTTT TCACTGACTT GGAGATTCTC GCGGCCATTT TTGCCAGTGC AATACATGAT 
GTGGATCATC CTGGTGTGTC AAATCAATTT CTGATCAATA CAAACTCGGA ACTTGCCTTG 
ATGTACAACG ACTCCTCCGT CTTAGAGAAT CATCATTTGG CTGTGGGCTT TAAGTTGCTC 
CAGGAAGAAA ACTGTGACAT TTTCCAGAAT CTGACCAAAA AGCAAAGACA ATCTTTAAGG 
AAAATGGCCA TTGACATTGT ACTAGCGACA GACATGTCAA AGCACATGAA TCTGCTGGCT 
GATCTGAAAA CAATGGTTGA AACGAAGAAG GTGACGAGCT CTGGCGTCCT CCTCCTTGAT 
AACTATTCTG ACAGGATCCA GGTCCTCCAG AATATGGTGC ACTGTGCAGA CCTGAGCAAC 
CCCACAAAGC CACTCCAGCT CTACCGCCAG TGGACGGACC GGATAATGGA GGAGTTCTTC 
CGTCAGGGGG ACCGGGAGCG TGAGCGTGGC ATGGAGATAA GTCCCATGTG TGACAAGCAC 
AACGCCTCTG TGGAAAAATC ACAGGTGGGC TTCATAGACT ACATTGTTCA TCCACTCTGG 
GAGACATGGG CAGACCTCGT ACATCCCGAC GCCCAGGACA TTTTGGACAC TTTGGAGGAC 
AATCGTGAGT GGTACCAGAG CACAATCCCC CAGAGCCCCT CCCCTGCACC TGATGACCAA 
GAGGACGGCC GTCAGGGACA GACTGAAAAA TTCCAGTTCG AACTAACCTT AGAGGAAGAT 
GGCGAGTCAG ACACTGAAAA GGACAGTGGA AGTCAAGTGG AGGAAGACAC TAGCTGCAGT 
GACTCTAAGA CTCTGTGCAC CCAAGACTCA GAGTCCACTG AAATTCCCCT TGACGAGCAG 
GTTGAAGAGG AGGCTGTAGC AGAAGAGGAA AGCCAGCCCC AAACTGGCGT CGCAGACGAT 
TGTTGTCCTG ACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde4d ( XM_008760721.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758943.1 Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X1, mRNA.

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CloneID	ORa42933
Clone ID Related Accession (Same CDS sequence)	XM_008760721.1
Accession Version	XM_008760721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTA TTATGAAGCC GAGATCCAGG TCCACAAGTT CCCTGAGGAC AACGGAGGCA 
GTTTGTTTCG ATGTGGACAA TGGCACATCA GCGGGACGGA GTCCCTTGGA TCCCATGACC 
AGCCCAGGGT CTGGGCTAAT TCTCCAAGCA AACTTTGTGC ACAGTCAACG CCGGGAGTCC 
TTCCTGTATC GATCAGACAG CGATTATGAC CTCTCTCCAA AGTCTATGTC CAGGAACTCC 
TCGATTGCCA GTGATATACA TGGAGATGAC TTGATTGTGA CTCCATTTGC TCAGGTCTTG 
GCCAGCCTTC GAACTGTAAG AAACAACTTT GCTGCATTAA CTAATTTGCA AGATCGCGCA 
CCCAGCAAAA GATCACCCAT GTGCAACCAA CCATCCATCA ACAAAGCCAC CATCACAGAG 
GAGGCCTACC AGAAACTGGC CAGCGAGACC CTGGAGGAGC TGGACTGGTG TCTGGACCAG 
CTAGAGACCC TGCAGACCAG GCACTCCGTC AGTGAGATGG CCTCCAACAA GTTCAAGAGG 
ATGCTTAATC GGGAGCTCAC CCACCTCTCT GAAATGAGTC GGTCTGGCAA CCAGGTGTCG 
GAGTACATAT CAAACACGTT CTTAGATAAG CAACATGAAG TAGAAATTCC CTCTCCGACT 
CAGAAGGAAA AAGAGAAGAA GAAAAGGCCG ATGTCACAGA TCAGTGGGGT CAAGAAGTTG 
ATGCACAGCT CCAGCCTGAC CAATTCCTGC ATCCCAAGAT TTGGGGTTAA AACAGAGCAG 
GAAGATGTCC TGGCCAAGGA ACTAGAAGAC GTGAACAAGT GGGGCCTCCA CGTTTTCCGA 
ATAGCGGAGC TGTCTGGCAA CCGGCCTCTG ACTGTTATCA TGCACACCAT TTTTCAGGAA 
CGAGATTTGT TAAAAACGTT TAAAATCCCA GTGGACACTT TGATTACGTA TCTTATGACT 
CTAGAAGACC ATTACCATGC TGACGTGGCC TATCACAACA ACATCCATGC TGCAGATGTC 
GTCCAGTCAA CTCATGTGCT GCTCTCTACA CCCGCTTTGG AGGCTGTTTT CACTGACTTG 
GAGATTCTCG CGGCCATTTT TGCCAGTGCA ATACATGATG TGGATCATCC TGGTGTGTCA 
AATCAATTTC TGATCAATAC AAACTCGGAA CTTGCCTTGA TGTACAACGA CTCCTCCGTC 
TTAGAGAATC ATCATTTGGC TGTGGGCTTT AAGTTGCTCC AGGAAGAAAA CTGTGACATT 
TTCCAGAATC TGACCAAAAA GCAAAGACAA TCTTTAAGGA AAATGGCCAT TGACATTGTA 
CTAGCGACAG ACATGTCAAA GCACATGAAT CTGCTGGCTG ATCTGAAAAC AATGGTTGAA 
ACGAAGAAGG TGACGAGCTC TGGCGTCCTC CTCCTTGATA ACTATTCTGA CAGGATCCAG 
GTCCTCCAGA ATATGGTGCA CTGTGCAGAC CTGAGCAACC CCACAAAGCC ACTCCAGCTC 
TACCGCCAGT GGACGGACCG GATAATGGAG GAGTTCTTCC GTCAGGGGGA CCGGGAGCGT 
GAGCGTGGCA TGGAGATAAG TCCCATGTGT GACAAGCACA ACGCCTCTGT GGAAAAATCA 
CAGGTGGGCT TCATAGACTA TATTGTTCAT CCACTCTGGG AGACATGGGC AGACCTCGTA 
CATCCCGACG CCCAGGACAT TTTGGACACT TTGGAGGACA ATCGTGAGTG GTACCAGAGC 
ACAATCCCCC AGAGCCCCTC CCCTGCACCT GATGACCAAG AGGACGGCCG TCAGGGACAG 
ACTGAAAAAT TCCAGTTCGA ACTAACCTTA GAGGAAGATG GCGAGTCAGA CACTGAAAAG 
GACAGTGGAA GTCAAGTGGA GGAAGACACT AGCTGCAGTG ACTCTAAGAC TCTGTGCACC 
CAAGACTCAG AGTCCACTGA AATTCCCCTT GACGAGCAGG TTGAAGAGGA GGCTGTAGCA 
GAAGAGGAAA GCCAGCCCCA AACTGGCGTC GCAGACGATT GTTGTCCTGA CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758943.1
CDS	1384..3420
Translation	MSIIMKPRSRSTSSLRTTEAVCFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT

Target ORF information:

RefSeq Version	XM_008760721.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4D (Pde4d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760721.1

ORF Insert Sequence:

ATGAGCATTA TTATGAAGCC GAGATCCAGG TCCACAAGTT CCCTGAGGAC AACGGAGGCA 
GTTTGTTTCG ATGTGGACAA TGGCACATCA GCGGGACGGA GTCCCTTGGA TCCCATGACC 
AGCCCAGGGT CTGGGCTAAT TCTCCAAGCA AACTTTGTGC ACAGTCAACG CCGGGAGTCC 
TTCCTGTATC GATCAGACAG CGATTATGAC CTCTCTCCAA AGTCTATGTC CAGGAACTCC 
TCGATTGCCA GTGATATACA TGGAGATGAC TTGATTGTGA CTCCATTTGC TCAGGTCTTG 
GCCAGCCTTC GAACTGTAAG AAACAACTTT GCTGCATTAA CTAATTTGCA AGATCGCGCA 
CCCAGCAAAA GATCACCCAT GTGCAACCAA CCATCCATCA ACAAAGCCAC CATCACAGAG 
GAGGCCTACC AGAAACTGGC CAGCGAGACC CTGGAGGAGC TGGACTGGTG TCTGGACCAG 
CTAGAGACCC TGCAGACCAG GCACTCCGTC AGTGAGATGG CCTCCAACAA GTTCAAGAGG 
ATGCTTAATC GGGAGCTCAC CCACCTCTCT GAAATGAGTC GGTCTGGCAA CCAGGTGTCG 
GAGTACATAT CAAACACGTT CTTAGATAAG CAACATGAAG TAGAAATTCC CTCTCCGACT 
CAGAAGGAAA AAGAGAAGAA GAAAAGGCCG ATGTCACAGA TCAGTGGGGT CAAGAAGTTG 
ATGCACAGCT CCAGCCTGAC CAATTCCTGC ATCCCAAGAT TTGGGGTTAA AACAGAGCAG 
GAAGATGTCC TGGCCAAGGA ACTAGAAGAC GTGAACAAGT GGGGCCTCCA CGTTTTCCGA 
ATAGCGGAGC TGTCTGGCAA CCGGCCTCTG ACTGTTATCA TGCACACCAT TTTTCAGGAA 
CGAGATTTGT TAAAAACGTT TAAAATCCCA GTGGACACTT TGATTACGTA TCTTATGACT 
CTAGAAGACC ATTACCATGC TGACGTGGCC TATCACAACA ACATCCATGC TGCAGATGTC 
GTCCAGTCAA CTCATGTGCT GCTCTCTACA CCCGCTTTGG AGGCTGTTTT CACTGACTTG 
GAGATTCTCG CGGCCATTTT TGCCAGTGCA ATACATGATG TGGATCATCC TGGTGTGTCA 
AATCAATTTC TGATCAATAC AAACTCGGAA CTTGCCTTGA TGTACAACGA CTCCTCCGTC 
TTAGAGAATC ATCATTTGGC TGTGGGCTTT AAGTTGCTCC AGGAAGAAAA CTGTGACATT 
TTCCAGAATC TGACCAAAAA GCAAAGACAA TCTTTAAGGA AAATGGCCAT TGACATTGTA 
CTAGCGACAG ACATGTCAAA GCACATGAAT CTGCTGGCTG ATCTGAAAAC AATGGTTGAA 
ACGAAGAAGG TGACGAGCTC TGGCGTCCTC CTCCTTGATA ACTATTCTGA CAGGATCCAG 
GTCCTCCAGA ATATGGTGCA CTGTGCAGAC CTGAGCAACC CCACAAAGCC ACTCCAGCTC 
TACCGCCAGT GGACGGACCG GATAATGGAG GAGTTCTTCC GTCAGGGGGA CCGGGAGCGT 
GAGCGTGGCA TGGAGATAAG TCCCATGTGT GACAAGCACA ACGCCTCTGT GGAAAAATCA 
CAGGTGGGCT TCATAGACTA TATTGTTCAT CCACTCTGGG AGACATGGGC AGACCTCGTA 
CATCCCGACG CCCAGGACAT TTTGGACACT TTGGAGGACA ATCGTGAGTG GTACCAGAGC 
ACAATCCCCC AGAGCCCCTC CCCTGCACCT GATGACCAAG AGGACGGCCG TCAGGGACAG 
ACTGAAAAAT TCCAGTTCGA ACTAACCTTA GAGGAAGATG GCGAGTCAGA CACTGAAAAG 
GACAGTGGAA GTCAAGTGGA GGAAGACACT AGCTGCAGTG ACTCTAAGAC TCTGTGCACC 
CAAGACTCAG AGTCCACTGA AATTCCCCTT GACGAGCAGG TTGAAGAGGA GGCTGTAGCA 
GAAGAGGAAA GCCAGCCCCA AACTGGCGTC GCAGACGATT GTTGTCCTGA CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterologous desensitization of cardiac ?-adrenergic signal via hormone-induced ?AR/arrestin/PDE4 complexes.
Cardiovascular research113(6)656-670(2017 May)
Shi Q,Li M,Mika D,Fu Q,Kim S,Phan J,Shen A,Vandecasteele G,Xiang YK

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.
Diabetes64(9)3189-202(2015 Sep)
Kim MJ,Park SK,Lee JH,Jung CY,Sung DJ,Park JH,Yoon YS,Park J,Park KG,Song DK,Cho H,Kim ST,Koo SH

Heterozygous mutations in cyclic AMP phosphodiesterase-4D (PDE4D) and protein kinase A (PKA) provide new insights into the molecular pathology of acrodysostosis.
Cellular signalling26(11)2446-59(2014 Nov)
Kaname T,Ki CS,Niikawa N,Baillie GS,Day JP,Yamamura K,Ohta T,Nishimura G,Mastuura N,Kim OH,Sohn YB,Kim HW,Cho SY,Ko AR,Lee JY,Kim HW,Ryu SH,Rhee H,Yang KS,Joo K,Lee J,Kim CH,Cho KH,Kim D,Yanagi K,Naritomi K,Yoshiura K,Kondoh T,Nii E,Tonoki H,Houslay MD,Jin DK

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Pde4d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

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