Dmgdh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dmgdh
Entrez Gene ID	245961
Full Name	dimethylglycine dehydrogenase
Synonyms	Me2GlyDH
General protein information	Preferred Names dimethylglycine dehydrogenase Names dimethylglycine dehydrogenase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	Enzyme involved in choline catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760630.1	XP_008758852.1	dimethylglycine dehydrogenase, mitochondrial isoform X2
NM_139102.2	NP_620802.2	dimethylglycine dehydrogenase, mitochondrial precursor
XM_008760629.2	XP_008758851.1	dimethylglycine dehydrogenase, mitochondrial isoform X1
XM_008760632.1	XP_008758854.1	dimethylglycine dehydrogenase, mitochondrial isoform X4
XM_008760631.2	XP_008758853.1	dimethylglycine dehydrogenase, mitochondrial isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dmgdh	NP_083048.1
Danio rerio (zebrafish)	dmgdh	XP_002665871.2
Pan troglodytes (chimpanzee)	DMGDH	XP_526883.2
Canis lupus familiaris (dog)	DMGDH	XP_005618235.1
Gallus gallus (chicken)	DMGDH	XP_003643002.1
Caenorhabditis elegans (roundworm)	Y37E3.17	NP_001021746.1
Xenopus tropicalis (tropical clawed frog)	dmgdh	XP_002940239.2
Homo sapiens (human)	DMGDH	NP_037523.2
Rattus norvegicus (Norway rat)	Dmgdh	NP_620802.2
Bos taurus (cattle)	DMGDH	NP_001192474.1

Gene Ontology
Bibliography

Related articles in PubMed

Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate.
Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C
Biochemical and biophysical research communications449(4)392-8(2014 Jul)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Compartmentalization of Mammalian folate-mediated one-carbon metabolism.
Tibbetts AS, Appling DR
Annual review of nutrition3057-81(2010 Aug)

The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction.
Brizio C, Brandsch R, Douka M, Wait R, Barile M
International journal of biological macromolecules42(5)455-62(2008 Jun)

Over-expression in Escherichia coli, functional characterization and refolding of rat dimethylglycine dehydrogenase.
Brizio C, Brandsch R, Bufano D, Pochini L, Indiveri C, Barile M
Protein expression and purification37(2)434-42(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

crystal structures of mature and precursor forms of DMGDH in presence of tetrahydrofolate; folate binding site is connected with enzyme active center via an intramolecular channel suggesting possible transfer of the intermediate imine of dimethylglycine from the active center to the bound THF where they could react producing a 5,10-methylenetetrahydrofolate
Title: Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate.

covalent attachment of FAD to dimethylglycine dehydrogenase apoenzyme can proceed in vitro autocatalytically
Title: The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction.

characterization and folding of rat dimethylglycine dehydrogenase
Title: Over-expression in Escherichia coli, functional characterization and refolding of rat dimethylglycine dehydrogenase.

The following Dmgdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dmgdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02172	NM_139102.2 Latest version!	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa44397	XM_008760629.2 Latest version!	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44398	XM_008760630.1 Latest version!	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44399	XM_008760631.2 Latest version!	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44400	XM_008760632.1 Latest version!	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dmgdh ( NM_139102.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620802.2 Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), mRNA.

Price & Availability↑

CloneID	ORa02172
Clone ID Related Accession (Same CDS sequence)	NM_139102.2
Accession Version	NM_139102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	dimethylglycine dehydrogenase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085697.1. On or before Jan 12, 2005 this sequence version replaced XM_346609.1, NM_139102.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085697.1, X55995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CCGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGC CAGCCAACTT CACAGGGAAG CAAGCGCTGA AGCAGATTAA AGCCAAGGGG 
CTGAAGCGGA GACTGGTGTG CCTCACCTTG GCAACAGACA ATGTGGATCC AGAGGGAAAT 
GAAAGCGTTT GGTACAAGGG CAAGGTGATT GGCAACACAA CATCTGGAAG CTACAGTTAC 
AGCATCCAGA AGAGCCTGGC TTTTGCTTAT GTCCCAGTTG AGCTCAGTGA AGTGGGACAA 
CAAGTGGAGG TTGAACTACT GGGTAAAAAT TACCCAGCAA CCATTATACA GGAACCATTG 
GTACTCACAG AACCTACCAG AACCAGGCTT CAGAAAGATG GTAGGAAATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620802.2
CDS	33..2606
Translation	MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKPANFTGKQALKQIKAKGLKRRLVCLTLATDNVDPEGNESVWYKGKVIGNTTSGSYSYSIQKSLAFAYVPVELSEVGQQVEVELLGKNYPATIIQEPLVLTEPTRTRLQKDGRKS

Target ORF information:

RefSeq Version	NM_139102.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139102.2

ORF Insert Sequence:

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CCGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGC CAGCCAACTT CACAGGGAAG CAAGCGCTGA AGCAGATTAA AGCCAAGGGG 
CTGAAGCGGA GACTGGTGTG CCTCACCTTG GCAACAGACA ATGTGGATCC AGAGGGAAAT 
GAAAGCGTTT GGTACAAGGG CAAGGTGATT GGCAACACAA CATCTGGAAG CTACAGTTAC 
AGCATCCAGA AGAGCCTGGC TTTTGCTTAT GTCCCAGTTG AGCTCAGTGA AGTGGGACAA 
CAAGTGGAGG TTGAACTACT GGGTAAAAAT TACCCAGCAA CCATTATACA GGAACCATTG 
GTACTCACAG AACCTACCAG AACCAGGCTT CAGAAAGATG GTAGGAAATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmgdh ( XM_008760629.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758851.1 Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44397
Clone ID Related Accession (Same CDS sequence)	XM_008760629.2
Accession Version	XM_008760629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGA GAGATGAACA GTCCAGCCAT GCCTGGACAC TGAAGCCGCA GCCCCTCCAG 
CATACAGAAA TCTCCACGTT CTGGGGAGAA GCACAGCATT TGCTTCTGAG CCTTCCAAAC 
CTTCCTGTGC GTTTCATAAG CGCGGAAATC GTAGCACAAC ACAAAGGCAC ATGGGGCCAT 
CTTGGAGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758851.1
CDS	48..2459
Translation	MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKRDEQSSHAWTLKPQPLQHTEISTFWGEAQHLLLSLPNLPVRFISAEIVAQHKGTWGHLGV

Target ORF information:

RefSeq Version	XM_008760629.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760629.2

ORF Insert Sequence:

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGA GAGATGAACA GTCCAGCCAT GCCTGGACAC TGAAGCCGCA GCCCCTCCAG 
CATACAGAAA TCTCCACGTT CTGGGGAGAA GCACAGCATT TGCTTCTGAG CCTTCCAAAC 
CTTCCTGTGC GTTTCATAAG CGCGGAAATC GTAGCACAAC ACAAAGGCAC ATGGGGCCAT 
CTTGGAGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmgdh ( XM_008760630.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758852.1 Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44398
Clone ID Related Accession (Same CDS sequence)	XM_008760630.1
Accession Version	XM_008760630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGC ATCTGGGTCA CGCCGTGAAG GGAGAGAGAC CTGGACTTTG GTTTTGTACA 
GTATTTAAAT CCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758852.1
CDS	48..2345
Translation	MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKHLGHAVKGERPGLWFCTVFKSC

Target ORF information:

RefSeq Version	XM_008760630.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760630.1

ORF Insert Sequence:

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGC ATCTGGGTCA CGCCGTGAAG GGAGAGAGAC CTGGACTTTG GTTTTGTACA 
GTATTTAAAT CCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmgdh ( XM_008760631.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758853.1 Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44399
Clone ID Related Accession (Same CDS sequence)	XM_008760631.2
Accession Version	XM_008760631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGA TTTTCGACAC AGACAACCAC GTTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758853.1
CDS	48..2306
Translation	MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKIFDTDNHVV

Target ORF information:

RefSeq Version	XM_008760631.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760631.2

ORF Insert Sequence:

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGA TTTTCGACAC AGACAACCAC GTTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmgdh ( XM_008760632.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758854.1 Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa44400
Clone ID Related Accession (Same CDS sequence)	XM_008760632.1
Accession Version	XM_008760632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dimethylglycine dehydrogenase, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGT TCCTCCCTCG GCTTAACCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758854.1
CDS	48..2303
Translation	MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKFLPRLNPP

Target ORF information:

RefSeq Version	XM_008760632.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760632.1

ORF Insert Sequence:

ATGCTCCGCC TGGGCGCGCT CAGGCTGCGG GGCCTCGCGC TCCGGAGCTC CCAGGGGCGC 
CCAAGCTCCG CCGGCCTCCG GGAAGGACAG GAAAGCCCAC CCTCACCCCC AGAATGGAAG 
GACAGAGCAG AGACTGTGAT CATTGGAGGT GGCTGCGTGG GCGTGAGTCT GGCTTACCAT 
CTGGCCAAGG CAGGGATGAG AGACGTGGTC CTGTTGGAGA AGTCGGAACT CACGGCTGGC 
TCTACCTGGC ACGCAGCAGG TTTAACAACT TACTTTCACC CCGGGATAAA CTTGAAGAAG 
ATACATTATG ACAGCATCAA ACTTTACGAG AGGCTGGAAG AAGAAACTGG ACAGGTGGTG 
GGGTTCCATC AACCGGGTAG TATCAGGCTT GCGACAACTC CTGAAAGGGT GGACGAATTT 
AAATACCAAA TGACGCGAAC CAACTGGCAT GCCACCGAGC AGTATATTAT TGAGCCCGAA 
AAAATCCACG AGCTGTTCCC TTTACTCAAC ATGGACAAGA TTTTAGCCGG ACTGTATAAC 
CCGGGAGATG GCCACATCGA TCCATATTCT CTGACGATGG CCCTGGCTAC TGGAGCTAGG 
AAATATGGGG CCCTTCTAAA GTATCCTGCA CCAGTGACAT CTCTGAAGCC CAGGCCAGAT 
GGGACATGGG ACGTGGAGAC TCCGCAAGGA TCTGTGAGAG CAAATCGAAT TGTGAATGCT 
GCGGGATTTT GGGCTCGTGA AGTGGGTAAA ATGATTGGAC TAGACCATCC CCTCATCCCT 
GTGCAACATC AATATGTCAT CACATCAACA ATCCCAGAAG TGAAGGCTTT GAAACGAGAA 
CTCCCAGTCC TTCGTGACTT GGAAGGATCC TACTACCTCC GACAGGAAAG GGACGGGCTT 
TTGTTTGGTC CCTATGAAAG TCAGGAGAAG ATGAAACTTC AAGCCTCCTG GGTCGCTCAT 
GGAGTCCCTC CAGGCTTTGG GAAGGAACTT TTTGAGTCTG ACTTAGACCG AATCACAGAG 
CACGTGGAAG CTGCCATGGA AATGGTTCCT GTTCTGAAAA AGGCGGACAT CATCAACATC 
GTCAACGGTC CTATCACCTA CTCTCCTGAC ATTCTGCCTA TGGTGGGGCC CCATCAGGGG 
GTCAGGAACT ACTGGGTGGC CATTGGCTTT GGATATGGCA TAATCCACGC CGGTGGGGTC 
GGGACGTACC TCAGCGACTG GATCCTGCAC GGAGAACCTC CCTTTGACCT GATAGAACTG 
GATCCCAACC GCTACGGCAA GTGGACGACG ACGCAGTACA CCGAGGCCAA AGCCCGAGAA 
TCATACGGGT TCAACAACAT TGTTGGTTAT CCTAAAGAGG AACGGTTTGC TGGGAGGCCA 
ACCCAGAGAG TCAGTGGGCT CTACAAAATC CTGGAGTCCA AGTGTTCCAT GGGTTTCCAT 
GCAGGCTGGG AACAGCCACA CTGGTTCTAC AAGCCCGGCC AGGACACTCA GTATAGGCCA 
AGTTTCCGCC GCACAAACTG GTTTGAGCCG GTGGGCTCTG AGTACAAACA GGTTATGCAA 
AGAGTTGGTG TGATTGACCT GTCTCCGTTT GGCAAGTTCA ACATCAAAGG CCAAGATTCC 
ACCCAGCTTC TGGATCATCT CTGTGCAAAT GTCATTCCCA AGGTGGGTTT TACAAACATA 
AGTCACATGT TGACGCCCAG AGGTCGAGTG TACGCTGAGC TGACTGTTTC CCACCAATCT 
CCTGGGGAGT TCCTGTTGAT TACTGGCTCT GGGTCGGAAC TTCACGATCT TAGATGGATT 
GAAGAAGCAG CCGTCAGAGG TGGCTATGAT GTGGAAATTC GAAACATAAC CGATGAGCTT 
GGGGTCCTGG GAGTAGCAGG GCCATATGCA AGGAGAGTCC TTCAGAAGCT GACCTCAGAA 
GATCTCAGTG ATGATGTCTT CAAGTTTCTT CAAACCAAGT CTTTAAAGAT CTCTGACATT 
CCTGTCACTG CTATCAGGAT CTCCTATACT GGTGAGCTGG GATGGGAGCT GTACCACAGA 
CGAGAAGATT CTGCAGCCCT TTATGAGCGG ATCATGAATG CGGGCCAGGA GGAGGGCATC 
GACAATTTTG GAACATATGC CCTGAATGCC TTAAGACTGG AGAAGGCTTT CAGGGCCTGG 
GGGTCAGAGA TGAACTGTGA CACAAACCCT TTGGAAGCTG GGCTGGACTA TTTCATCAAG 
CTGAACAAGT TCCTCCCTCG GCTTAACCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate.
Biochemical and biophysical research communications449(4)392-8(2014 Jul)
Luka Z,Pakhomova S,Loukachevitch LV,Newcomer ME,Wagner C

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Compartmentalization of Mammalian folate-mediated one-carbon metabolism.
Annual review of nutrition3057-81(2010 Aug)
Tibbetts AS,Appling DR

The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction.
International journal of biological macromolecules42(5)455-62(2008 Jun)
Brizio C,Brandsch R,Douka M,Wait R,Barile M

Over-expression in Escherichia coli, functional characterization and refolding of rat dimethylglycine dehydrogenase.
Protein expression and purification37(2)434-42(2004 Oct)
Brizio C,Brandsch R,Bufano D,Pochini L,Indiveri C,Barile M