Mbl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mbl1
Entrez Gene ID	24548
Full Name	mannose-binding lectin (protein A) 1
Synonyms	Mbpa,Mlb1
General protein information	Preferred Names mannose-binding lectin (protein A) 1 Names mannose-binding protein A MBP-A Mannose binding protein A, serum mannan-binding protein mannose binding lectin (A) mannose binding lectin 1, protein A mannose binding lectin 2, protein C
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	N-terminal region has similarity to nonfibrillar collagens; C-terminal region, which includes the carbohydrate-binding domain, has similarity to the asialoglycoprotein receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012599.2	NP_036731.2	mannose-binding protein A precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mbl1	NP_036731.2
Pan troglodytes (chimpanzee)	LOC466126	XP_521528.2
	MBP-A	XP_001096399.1
Canis lupus familiaris (dog)	LOC479260	XP_536402.2
Mus musculus (house mouse)	Mbl1	NP_034905.1
Bos taurus (cattle)	MBL1	NP_001010994.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of mannose-binding lectin in the pathogenesis of Kawasaki disease-like murine vasculitis.
Nakamura A, Okigaki M, Miura N, Suzuki C, Ohno N, Kametani F, Hamaoka K
Clinical immunology (Orlando, Fla.)153(1)64-72(2014 Jul)

Targeting mannose-binding lectin confers long-lasting protection with a surprisingly wide therapeutic window in cerebral ischemia.
Orsini F, Villa P, Parrella S, Zangari R, Zanier ER, Gesuete R, Stravalaci M, Fumagalli S, Ottria R, Reina JJ, Paladini A, Micotti E, Ribeiro-Viana R, Rojo J, Pavlov VI, Stahl GL, Bernardi A, Gobbi M, De Simoni MG
Circulation126(12)1484-94(2012 Sep)

Near-planar solution structures of mannose-binding lectin oligomers provide insight on activation of lectin pathway of complement.
Miller A, Phillips A, Gor J, Wallis R, Perkins SJ
The Journal of biological chemistry287(6)3930-45(2012 Feb)

[Relationship between activation of mannan-binding lectin complement and NF-kappaB in diabetic nephropathy].
Yang YX, Huang SM, Yan XY, Wu WH
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition42(4)490-3(2011 Jul)

The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart.
La Bonte LR, Dokken B, Davis-Gorman G, Stahl GL, McDonagh PF
Diabetes & vascular disease research6(3)172-80(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data show an important role for MBL in the pathogenesis of brain ischemic injury and provide a strong support to the concept that MBL inhibition may be a relevant therapeutic target.
Title: Targeting mannose-binding lectin confers long-lasting protection with a surprisingly wide therapeutic window in cerebral ischemia.

The expression of MBL was positively correlated with NF-kappaB expression in diabetic nephropathy.
Title: [Relationship between activation of mannan-binding lectin complement and NF-kappaB in diabetic nephropathy].

MASP-1 probably enhances complement activation triggered by MBP.MASP-2 complexes, but it cannot initiate activation itself
Title: Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases.

that the triple helical structure of the collagen-like domain is largely intact in the mutant proteins, but it is more easily unfolded than in wild-type MBP
Title: Localization of the serine protease-binding sites in the collagen-like domain of mannose-binding protein: indirect effects of naturally occurring mutations on protease binding and activation.

The following Mbl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mbl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00426	NM_012599.2 Latest version!	Rattus norvegicus mannose-binding lectin (protein A) 1 (Mbl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mbl1 ( NM_012599.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036731.2 Rattus norvegicus mannose-binding lectin (protein A) 1 (Mbl1), mRNA.

Price & Availability↑

CloneID	ORa00426
Clone ID Related Accession (Same CDS sequence)	NM_012599.2
Accession Version	NM_012599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	mannose-binding protein A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088159.1. On Mar 13, 2005 this sequence version replaced NM_012599.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088159.1, FQ218582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCTGC TTCCACTGCT CGTCCTTCTC TGTGTAGTGA GCGTGTCCTC ATCAGGGTCA 
CAAACCTGTG AGGAAACCCT GAAGACTTGC TCTGTGATAG CCTGCGGCAG AGACGGGAGA 
GATGGGCCCA AAGGGGAGAA GGGAGAACCA GGTCAAGGGC TCAGGGGCTT GCAGGGCCCT 
CCAGGGAAAC TGGGGCCTCC AGGAAGTGTA GGAGCCCCTG GAAGTCAAGG ACCAAAAGGC 
CAAAAAGGGG ATCGTGGAGA CAGCAGAGCC ATTGAGGTGA AGCTGGCAAA TATGGAGGCA 
GAGATAAACA CCCTGAAGTC AAAACTGGAG CTAACCAACA AGTTGCATGC CTTCTCCATG 
GGTAAAAAGT CTGGGAAGAA GTTCTTTGTG ACCAACCATG AAAGGATGCC CTTTTCCAAA 
GTCAAGGCCC TGTGCTCAGA GCTCCGAGGC ACTGTGGCTA TCCCCAGGAA TGCTGAGGAG 
AACAAGGCCA TCCAAGAAGT GGCTAAAACC TCTGCCTTCC TAGGCATCAC GGACGAGGTG 
ACTGAAGGCC AATTCATGTA TGTGACAGGG GGGAGGCTCA CCTACAGCAA CTGGAAAAAG 
GATGAGCCCA ATGACCATGG CTCTGGGGAA GACTGTGTCA CTATAGTAGA CAACGGTCTG 
TGGAATGACA TCTCCTGCCA AGCTTCCCAC ACGGCTGTCT GCGAGTTCCC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036731.2
CDS	73..789
Translation	MLLLPLLVLLCVVSVSSSGSQTCEETLKTCSVIACGRDGRDGPKGEKGEPGQGLRGLQGPPGKLGPPGSVGAPGSQGPKGQKGDRGDSRAIEVKLANMEAEINTLKSKLELTNKLHAFSMGKKSGKKFFVTNHERMPFSKVKALCSELRGTVAIPRNAEENKAIQEVAKTSAFLGITDEVTEGQFMYVTGGRLTYSNWKKDEPNDHGSGEDCVTIVDNGLWNDISCQASHTAVCEFPA

Target ORF information:

RefSeq Version	NM_012599.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannose-binding lectin (protein A) 1 (Mbl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012599.2

ORF Insert Sequence:

ATGCTCCTGC TTCCACTGCT CGTCCTTCTC TGTGTAGTGA GCGTGTCCTC ATCAGGGTCA 
CAAACCTGTG AGGAAACCCT GAAGACTTGC TCTGTGATAG CCTGCGGCAG AGACGGGAGA 
GATGGGCCCA AAGGGGAGAA GGGAGAACCA GGTCAAGGGC TCAGGGGCTT GCAGGGCCCT 
CCAGGGAAAC TGGGGCCTCC AGGAAGTGTA GGAGCCCCTG GAAGTCAAGG ACCAAAAGGC 
CAAAAAGGGG ATCGTGGAGA CAGCAGAGCC ATTGAGGTGA AGCTGGCAAA TATGGAGGCA 
GAGATAAACA CCCTGAAGTC AAAACTGGAG CTAACCAACA AGTTGCATGC CTTCTCCATG 
GGTAAAAAGT CTGGGAAGAA GTTCTTTGTG ACCAACCATG AAAGGATGCC CTTTTCCAAA 
GTCAAGGCCC TGTGCTCAGA GCTCCGAGGC ACTGTGGCTA TCCCCAGGAA TGCTGAGGAG 
AACAAGGCCA TCCAAGAAGT GGCTAAAACC TCTGCCTTCC TAGGCATCAC GGACGAGGTG 
ACTGAAGGCC AATTCATGTA TGTGACAGGG GGGAGGCTCA CCTACAGCAA CTGGAAAAAG 
GATGAGCCCA ATGACCATGG CTCTGGGGAA GACTGTGTCA CTATAGTAGA CAACGGTCTG 
TGGAATGACA TCTCCTGCCA AGCTTCCCAC ACGGCTGTCT GCGAGTTCCC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of mannose-binding lectin in the pathogenesis of Kawasaki disease-like murine vasculitis.
Clinical immunology (Orlando, Fla.)153(1)64-72(2014 Jul)
Nakamura A,Okigaki M,Miura N,Suzuki C,Ohno N,Kametani F,Hamaoka K

Targeting mannose-binding lectin confers long-lasting protection with a surprisingly wide therapeutic window in cerebral ischemia.
Circulation126(12)1484-94(2012 Sep)
Orsini F,Villa P,Parrella S,Zangari R,Zanier ER,Gesuete R,Stravalaci M,Fumagalli S,Ottria R,Reina JJ,Paladini A,Micotti E,Ribeiro-Viana R,Rojo J,Pavlov VI,Stahl GL,Bernardi A,Gobbi M,De Simoni MG

Near-planar solution structures of mannose-binding lectin oligomers provide insight on activation of lectin pathway of complement.
The Journal of biological chemistry287(6)3930-45(2012 Feb)
Miller A,Phillips A,Gor J,Wallis R,Perkins SJ

[Relationship between activation of mannan-binding lectin complement and NF-kappaB in diabetic nephropathy].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition42(4)490-3(2011 Jul)
Yang YX,Huang SM,Yan XY,Wu WH

The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart.
Diabetes & vascular disease research6(3)172-80(2009 Jul)
La Bonte LR,Dokken B,Davis-Gorman G,Stahl GL,McDonagh PF