Irf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irf1
Entrez Gene ID	24508
Full Name	interferon regulatory factor 1
General protein information	Preferred Names interferon regulatory factor 1 Names interferon regulatory factor 1 IRF-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	stimulates cell proliferation and regulates growth-inhibitory interferon genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012591.1	NP_036723.1	interferon regulatory factor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IRF1	XP_001150404.2
Canis lupus familiaris (dog)	IRF1	XP_003639416.2
Bos taurus (cattle)	IRF1	NP_001178190.1
Mus musculus (house mouse)	Irf1	NP_001152868.1
Homo sapiens (human)	IRF1	NP_002189.1
Rattus norvegicus (Norway rat)	Irf1	NP_036723.1
Danio rerio (zebrafish)	irf1b	NP_991310.1
	IRF1	XP_001104294.1
Gallus gallus (chicken)	IRF1	NP_990746.1
Xenopus tropicalis (tropical clawed frog)	irf1	NP_001006695.1

Gene Ontology
Bibliography

Related articles in PubMed

An Up-regulation of IRF-1 After a Spinal Cord Injury: Implications for Neuronal Apoptosis.
Zhao J, Chen C, Xiao JR, Wei HF, Zhou XH, Mao XX, Zhang WD, Qian R, Chen XL, He MQ, Yu XW, Zhao J
Journal of molecular neuroscience : MN57(4)595-604(2015 Dec)

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation.
Qiu W, Zhou J, Zhu G, Zhao D, He F, Zhang J, Lu Y, Yu T, Liu L, Wang Y
Cell death & disease5e1176(2014 Apr)

Elevation in type I interferons inhibits HCN1 and slows cortical neuronal oscillations.
Stadler K, Bierwirth C, Stoenica L, Battefeld A, Reetz O, Mix E, Schuchmann S, Velmans T, Rosenberger K, Br?uer AU, Lehnardt S, Nitsch R, Budt M, Wolff T, Kole MH, Strauss U
Cerebral cortex (New York, N.Y. : 1991)24(1)199-210(2014 Jan)

Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells.
Zhang X, Liu L, Chen C, Chi YL, Yang XQ, Xu Y, Li XT, Guo SL, Xiong SH, Shen MR, Sun Y, Zhang CS, Hu KM
Cardiovascular diabetology12147(2013 Oct)

Interferon regulatory factor-1 as a positive regulator for high glucose-induced proliferation of vascular smooth muscle cells.
Yu S, Xi Z, Hai-Yan C, Ya-Li C, Shao-Hu X, Chuan-Sen Z, Xiang-Qun Y, Jin-Ping G, Hai-Yan L, Lei D
Journal of cellular biochemistry113(8)2671-8(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IRF-1 protein levels gradually increased after a spinal cord injury. IRF-1 depletion decreases neuronal apoptosis.
Title: An Up-regulation of IRF-1 After a Spinal Cord Injury: Implications for Neuronal Apoptosis.

These data implicate that sublytic C5b-9 induces the expression of both p300 and IRF-1, as well as p300-dependent IRF-1 acetylation that may contribute to XAF1 gene activation and subsequent glomerular mesangial cells apoptosis.
Title: Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation.

these findings identify HCN1 channels as a novel neural target for type I IFNs providing the possibility to tune neural responses
Title: Elevation in type I interferons inhibits HCN1 and slows cortical neuronal oscillations.

The Irf-1 overexpression-induced proliferation of VSMCs, the up-regulation of cyclin E/CDK2 and the acceleration of cell cycle progression are associated with ROS/Erk1/2 signaling pathway under high glucose conditions.
Title: Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells.

Overexpression of Irf-1 accelerated the proliferation of VSMCs, and down-regulation of Irf-1 depressed the proliferative ability of VSMCs under high-glucose conditions, indicating that Irf-1 was a positive regulator for high glucose-induced proliferation.
Title: Interferon regulatory factor-1 as a positive regulator for high glucose-induced proliferation of vascular smooth muscle cells.

The following Irf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13403	NM_012591.1 Latest version!	Rattus norvegicus interferon regulatory factor 1 (Irf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irf1 ( NM_012591.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036723.1 Rattus norvegicus interferon regulatory factor 1 (Irf1), mRNA.

Price & Availability↑

CloneID	ORa13403
Clone ID Related Accession (Same CDS sequence)	NM_012591.1
Accession Version	NM_012591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	interferon regulatory factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M34253.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M34253.1, BC076382.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCTATCA CTCGGATGCG AATGAGACCC TGGCTAGAGA TGCAGATTAA TTCCAACCAA 
ATTCCAGGGC TGAGCTGGAT CAATAAAGAA GAGATGATCT TCCAGATCCC ATGGAAGCAT 
GCTGCCTTGC ACGGTTGGGA TATCAACAAG GATGCCTGTC TGTTCCGGAG CTGGGCCATT 
CACACAGGCC GATACAAAGC TGGGGAAAAA GAGCCAGATC CCAAGACTTG GAAGGCAAAC 
TTCCGGTGTG CCATGAACTC CCTACCAGAC ATCGAGGAAG TGAAGGACCA GAGCAGGAAC 
AAGGGCAGCT CTGCTGTACG CGTGTACCGG ATGCTGCCAC CCCTCACCAA GAACCAGAGG 
AAAGAGAGAA AGTCCAAGTC CAGCCGTGAC ACTAAGAGCA AAACCAAGAG GAAGCTGTGC 
GGAGATTCTA GCCCTGACAC CTTATCTGAC GGACTGAGCA GCTCTACTCT GCCTGATGAC 
CACAGCAGTT ACACAGCTCA GGGATACCTG GGTCAGGACT TGGACATGGA CAGGGACATT 
ACCCCAGCTC TGTCACCGTG CGTCGTCAGC AGCAGTCTCT CTGAGTGGCA TATGCAGATG 
GACATCATGC CAGACAGCAC CACTGATCTG TACAACTTGC AGGTGTCGCC CATGCCCTCC 
ACCTCTGAAG CTGCAACAGA TGAGGATGAG GAAGGGAAGT TACCTGAGGA CATCATGAAG 
CTCTTTGAAC AGTCTGAGTG GCAGCCGACG CACGTGGATG GCAAGGGATA CTTGCTCAAT 
GAACCAGGAG CCCAACTCTC TACTGTCTAT GGAGACTTCA GCTGCAAGGA GGAACCAGAG 
ATCGACAGCC CTGGAGGGGA CATCGAGATA GGCATACAGC GTGTCTTCAC AGAGATGAAG 
AATATGGACC CCGTCATGTG GATGGACACC CTGCTGGGCA ACTCTACCAG GCCGCCCTCC 
ATTCAGGCTA TTCCTTGTGC ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036723.1
CDS	198..1184
Translation	MPITRMRMRPWLEMQINSNQIPGLSWINKEEMIFQIPWKHAALHGWDINKDACLFRSWAIHTGRYKAGEKEPDPKTWKANFRCAMNSLPDIEEVKDQSRNKGSSAVRVYRMLPPLTKNQRKERKSKSSRDTKSKTKRKLCGDSSPDTLSDGLSSSTLPDDHSSYTAQGYLGQDLDMDRDITPALSPCVVSSSLSEWHMQMDIMPDSTTDLYNLQVSPMPSTSEAATDEDEEGKLPEDIMKLFEQSEWQPTHVDGKGYLLNEPGAQLSTVYGDFSCKEEPEIDSPGGDIEIGIQRVFTEMKNMDPVMWMDTLLGNSTRPPSIQAIPCAP

Target ORF information:

RefSeq Version	NM_012591.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interferon regulatory factor 1 (Irf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012591.1

ORF Insert Sequence:

ATGCCTATCA CTCGGATGCG AATGAGACCC TGGCTAGAGA TGCAGATTAA TTCCAACCAA 
ATTCCAGGGC TGAGCTGGAT CAATAAAGAA GAGATGATCT TCCAGATCCC ATGGAAGCAT 
GCTGCCTTGC ACGGTTGGGA TATCAACAAG GATGCCTGTC TGTTCCGGAG CTGGGCCATT 
CACACAGGCC GATACAAAGC TGGGGAAAAA GAGCCAGATC CCAAGACTTG GAAGGCAAAC 
TTCCGGTGTG CCATGAACTC CCTACCAGAC ATCGAGGAAG TGAAGGACCA GAGCAGGAAC 
AAGGGCAGCT CTGCTGTACG CGTGTACCGG ATGCTGCCAC CCCTCACCAA GAACCAGAGG 
AAAGAGAGAA AGTCCAAGTC CAGCCGTGAC ACTAAGAGCA AAACCAAGAG GAAGCTGTGC 
GGAGATTCTA GCCCTGACAC CTTATCTGAC GGACTGAGCA GCTCTACTCT GCCTGATGAC 
CACAGCAGTT ACACAGCTCA GGGATACCTG GGTCAGGACT TGGACATGGA CAGGGACATT 
ACCCCAGCTC TGTCACCGTG CGTCGTCAGC AGCAGTCTCT CTGAGTGGCA TATGCAGATG 
GACATCATGC CAGACAGCAC CACTGATCTG TACAACTTGC AGGTGTCGCC CATGCCCTCC 
ACCTCTGAAG CTGCAACAGA TGAGGATGAG GAAGGGAAGT TACCTGAGGA CATCATGAAG 
CTCTTTGAAC AGTCTGAGTG GCAGCCGACG CACGTGGATG GCAAGGGATA CTTGCTCAAT 
GAACCAGGAG CCCAACTCTC TACTGTCTAT GGAGACTTCA GCTGCAAGGA GGAACCAGAG 
ATCGACAGCC CTGGAGGGGA CATCGAGATA GGCATACAGC GTGTCTTCAC AGAGATGAAG 
AATATGGACC CCGTCATGTG GATGGACACC CTGCTGGGCA ACTCTACCAG GCCGCCCTCC 
ATTCAGGCTA TTCCTTGTGC ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Up-regulation of IRF-1 After a Spinal Cord Injury: Implications for Neuronal Apoptosis.
Journal of molecular neuroscience : MN57(4)595-604(2015 Dec)
Zhao J,Chen C,Xiao JR,Wei HF,Zhou XH,Mao XX,Zhang WD,Qian R,Chen XL,He MQ,Yu XW,Zhao J

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation.
Cell death & disease5e1176(2014 Apr)
Qiu W,Zhou J,Zhu G,Zhao D,He F,Zhang J,Lu Y,Yu T,Liu L,Wang Y

Elevation in type I interferons inhibits HCN1 and slows cortical neuronal oscillations.
Cerebral cortex (New York, N.Y. : 1991)24(1)199-210(2014 Jan)
Stadler K,Bierwirth C,Stoenica L,Battefeld A,Reetz O,Mix E,Schuchmann S,Velmans T,Rosenberger K,Br?uer AU,Lehnardt S,Nitsch R,Budt M,Wolff T,Kole MH,Strauss U

Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells.
Cardiovascular diabetology12147(2013 Oct)
Zhang X,Liu L,Chen C,Chi YL,Yang XQ,Xu Y,Li XT,Guo SL,Xiong SH,Shen MR,Sun Y,Zhang CS,Hu KM

Interferon regulatory factor-1 as a positive regulator for high glucose-induced proliferation of vascular smooth muscle cells.
Journal of cellular biochemistry113(8)2671-8(2012 Aug)
Yu S,Xi Z,Hai-Yan C,Ya-Li C,Shao-Hu X,Chuan-Sen Z,Xiang-Qun Y,Jin-Ping G,Hai-Yan L,Lei D