Il3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il3
Entrez Gene ID	24495
Full Name	interleukin 3
General protein information	Preferred Names interleukin 3 Names interleukin-3 IL-3 MCGF P-cell-stimulating factor hematopoietic growth factor mast cell growth factor multipotential colony-stimulating factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	cytokine that functions as a multi-lineage haemopoietic growth regulator [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246226.3	XP_006246288.1	interleukin-3 isoform X1
NM_031513.1	NP_113701.1	interleukin-3 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Il3	NP_113701.1
Mus musculus (house mouse)	Il3	NP_034686.2

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-29b inhibits migration and proliferation of vascular smooth muscle cells in neointimal formation.
Lee J, Lim S, Song BW, Cha MJ, Ham O, Lee SY, Lee C, Park JH, Bae Y, Seo HH, Seung M, Choi E, Hwang KC
Journal of cellular biochemistry116(4)598-608(2015 Apr)

IL3 rs40401 polymorphism and interaction with smoking in risk of asthma in Japanese women: the Kyushu Okinawa Maternal and Child Health study.
Miyake Y, Tanaka K, Arakawa M
Scandinavian journal of immunology79(6)410-4(2014 Jun)

IL3 SNP rs40401 variant is a risk factor for rhinoconjunctivitis in Japanese women: the Kyushu Okinawa maternal and child health study.
Miyake Y, Tanaka K, Arakawa M
Cytokine64(1)86-9(2013 Oct)

A dual role for the immune response in a mouse model of inflammation-associated lung cancer.
Dougan M, Li D, Neuberg D, Mihm M, Googe P, Wong KK, Dranoff G
The Journal of clinical investigation121(6)2436-46(2011 Jun)

Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats.
Nishihara T, Ochi M, Sugimoto K, Takahashi H, Yano H, Kumon Y, Ohnishi T, Tanaka J
Experimental neurology229(2)507-16(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings demonstrated that miR-29b successfully suppressed the proliferation and migration of smooth muscle cells induced by IL-3 stimulation, probably through targeting of Mcl-1 and MMP2.
Title: MicroRNA-29b inhibits migration and proliferation of vascular smooth muscle cells in neointimal formation.

Strategies aimed at blocking signaling through the CC chemokine receptor 3 (CCR3) and/or the common beta chain of the IL-3, IL-5 and GM-CSF receptor (betac) efficiently reduced eosinophilic inflammation in both animal models and in asthmatic patients
Title: Spatial and temporal expression of CCR3 and the common beta chain of the IL-3, IL-5 and GM-CSF receptor in the nasal epithelium and lymphoid tissues in a rat model of allergic rhinitis.

The following Il3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42689	NM_031513.1 Latest version!	Rattus norvegicus interleukin 3 (Il3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33017	XM_006246226.3 Latest version!	Rattus norvegicus interleukin 3 (Il3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il3 ( NM_031513.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113701.1 Rattus norvegicus interleukin 3 (Il3), mRNA.

Price & Availability↑

CloneID	ORa42689
Clone ID Related Accession (Same CDS sequence)	NM_031513.1
Accession Version	NM_031513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U81492.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U81492.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN02642678, SAMN02642682 [ECO:0000350] ##Evidence-Data-END##

ATGGTTCTTG CCAGCTCTAC CACCAGCATC CTCTGTATGC TGCTCCCGCT CCTGATGCTC 
TTCCACCAGG GACTCCAGAT TTCAGACAGG GGCTCAGATG CCCACCATTT ACTCAGGACG 
TTGGATTGCA GGACTATTGC CTTGGAGATT TTGGTGAAGC TCCCATATCC TCAGGTATCT 
GGACTCAATA ATAGTGACGA CAAAGCCAAT CTGAGGAATA GTACCTTGCG GAGAGTAAAC 
CTGGACGAAT TCCTAAAAAG CCAAGAGGAG TTTGATTCTC AGGACACAAC GGACATCAAG 
TCCAAACTTC AGAAACTTAA GTGTTGTATT CCTGCAGCTG CGAGCGACTC TGTGTTGCCA 
GGTGTCTACA ATAAAGATCT GGATGACTTT AAGAAGAAAC TGAGATTCTA CGTGATCCAT 
CTTAAGGACC TGCAGCCAGT GTCAGTCTCT AGACCACCTC AGCCCACATC TAGCTCTGAC 
AACTTTCGCC CTATGACCGT GGAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113701.1
CDS	24..533
Translation	MVLASSTTSILCMLLPLLMLFHQGLQISDRGSDAHHLLRTLDCRTIALEILVKLPYPQVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTTDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC

Target ORF information:

RefSeq Version	NM_031513.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 3 (Il3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031513.1

ORF Insert Sequence:

ATGGTTCTTG CCAGCTCTAC CACCAGCATC CTCTGTATGC TGCTCCCGCT CCTGATGCTC 
TTCCACCAGG GACTCCAGAT TTCAGACAGG GGCTCAGATG CCCACCATTT ACTCAGGACG 
TTGGATTGCA GGACTATTGC CTTGGAGATT TTGGTGAAGC TCCCATATCC TCAGGTATCT 
GGACTCAATA ATAGTGACGA CAAAGCCAAT CTGAGGAATA GTACCTTGCG GAGAGTAAAC 
CTGGACGAAT TCCTAAAAAG CCAAGAGGAG TTTGATTCTC AGGACACAAC GGACATCAAG 
TCCAAACTTC AGAAACTTAA GTGTTGTATT CCTGCAGCTG CGAGCGACTC TGTGTTGCCA 
GGTGTCTACA ATAAAGATCT GGATGACTTT AAGAAGAAAC TGAGATTCTA CGTGATCCAT 
CTTAAGGACC TGCAGCCAGT GTCAGTCTCT AGACCACCTC AGCCCACATC TAGCTCTGAC 
AACTTTCGCC CTATGACCGT GGAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il3 ( XM_006246226.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246288.1 Rattus norvegicus interleukin 3 (Il3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33017
Clone ID Related Accession (Same CDS sequence)	XM_006246226.3
Accession Version	XM_006246226.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTTG CCAGCTCTAC CACCAGCATC CTCTGCATGC TGCTTCCGCT CCTGATGCTC 
TTCCACCAGG GACTCCAGTT TTCAGGCAGT GCCCACCGTT TACTCAGGAC GTTGGATTGC 
AGGACTATTG CCTTGGAGAT TTTGGTGAAG CTCCCAGTAT CTGGACTCAA TAATAGTGAT 
GACAAAGCCA ATCTGAGGAA TAGTACCTTG CGGAGAGTAA ACCTGGACGA ATTCCTAAAA 
AGCCAAGAGG AGTTTGATTC TCAGGACACA ATGGACATCA AGTCCAAACT TCAGAAACTT 
AAGTGTTGTA TTCCTGCAGC TGCGAGCGAC TCTGTGCTGC CAGGTGTCTA CAATAAAGAT 
CTGGATGACT TTAAGAAGAA ACTGAGATTC TACGTGATCC ATCTTAAGGA CCTGCAGCCA 
GTGTCAGTCT CTAGACCACC TCAGCCCACA TCTAGCTCTG ACAACTTCCG CCCTATGACC 
GTGGAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246288.1
CDS	7..498
Translation	MVLASSTTSILCMLLPLLMLFHQGLQFSGSAHRLLRTLDCRTIALEILVKLPVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTMDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC

Target ORF information:

RefSeq Version	XM_006246226.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 3 (Il3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246226.3

ORF Insert Sequence:

ATGGTTCTTG CCAGCTCTAC CACCAGCATC CTCTGCATGC TGCTTCCGCT CCTGATGCTC 
TTCCACCAGG GACTCCAGTT TTCAGGCAGT GCCCACCGTT TACTCAGGAC GTTGGATTGC 
AGGACTATTG CCTTGGAGAT TTTGGTGAAG CTCCCAGTAT CTGGACTCAA TAATAGTGAT 
GACAAAGCCA ATCTGAGGAA TAGTACCTTG CGGAGAGTAA ACCTGGACGA ATTCCTAAAA 
AGCCAAGAGG AGTTTGATTC TCAGGACACA ATGGACATCA AGTCCAAACT TCAGAAACTT 
AAGTGTTGTA TTCCTGCAGC TGCGAGCGAC TCTGTGCTGC CAGGTGTCTA CAATAAAGAT 
CTGGATGACT TTAAGAAGAA ACTGAGATTC TACGTGATCC ATCTTAAGGA CCTGCAGCCA 
GTGTCAGTCT CTAGACCACC TCAGCCCACA TCTAGCTCTG ACAACTTCCG CCCTATGACC 
GTGGAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-29b inhibits migration and proliferation of vascular smooth muscle cells in neointimal formation.
Journal of cellular biochemistry116(4)598-608(2015 Apr)
Lee J,Lim S,Song BW,Cha MJ,Ham O,Lee SY,Lee C,Park JH,Bae Y,Seo HH,Seung M,Choi E,Hwang KC

IL3 rs40401 polymorphism and interaction with smoking in risk of asthma in Japanese women: the Kyushu Okinawa Maternal and Child Health study.
Scandinavian journal of immunology79(6)410-4(2014 Jun)
Miyake Y,Tanaka K,Arakawa M

IL3 SNP rs40401 variant is a risk factor for rhinoconjunctivitis in Japanese women: the Kyushu Okinawa maternal and child health study.
Cytokine64(1)86-9(2013 Oct)
Miyake Y,Tanaka K,Arakawa M

A dual role for the immune response in a mouse model of inflammation-associated lung cancer.
The Journal of clinical investigation121(6)2436-46(2011 Jun)
Dougan M,Li D,Neuberg D,Mihm M,Googe P,Wong KK,Dranoff G

Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats.
Experimental neurology229(2)507-16(2011 Jun)
Nishihara T,Ochi M,Sugimoto K,Takahashi H,Yano H,Kumon Y,Ohnishi T,Tanaka J