B4galt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	B4galt1
Entrez Gene ID	24390
Full Name	beta-1,4-galactosyltransferase 1
Synonyms	Ggtb2
General protein information	Preferred Names beta-1,4-galactosyltransferase 1 Names beta-1,4-galactosyltransferase 1 Glycoprotein-4-beta-galactosyltransferase 2 UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 b4Gal-T1 beta-1,4-GalTase 1 beta4Gal-T1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	plays a role in N-linked oligosaccharide biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053287.1	NP_445739.1	beta-1,4-galactosyltransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B4GALT1	XP_003312085.1
Bos taurus (cattle)	B4GALT1	NP_803478.1
Rattus norvegicus (Norway rat)	B4galt1	NP_445739.1
Gallus gallus (chicken)	B4GALT1	NP_990533.1
Mus musculus (house mouse)	B4galt1	NP_071641.1
Danio rerio (zebrafish)	zgc:154116	NP_001070727.2
Xenopus tropicalis (tropical clawed frog)	b4galt1.2	NP_001016664.1
Homo sapiens (human)	B4GALT1	NP_001488.2
	B4GALT1	XP_001100938.1
Canis lupus familiaris (dog)	B4GALT1	XP_538701.3
Xenopus tropicalis (tropical clawed frog)	b4galt1.1	NP_001120432.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Upregulation of ?-1,4-galactosyltransferase I in rat spinal cord with experimental autoimmune encephalomyelitis.
Zhao J, Gao Y, Cheng C, Yan M, Wang J
Journal of molecular neuroscience : MN49(3)437-45(2013 Mar)

The functional interaction between CDK11p58 and ?-1,4-galactosyltransferase I involved in astrocyte activation caused by lipopolysaccharide.
Liu X, Cheng C, Shao B, Wu X, Ji Y, Lu X, Shen A
Inflammation35(4)1365-77(2012 Aug)

?-1,4-Galactosyltransferase I involved in Schwann cells proliferation and apoptosis induced by tumor necrosis factor-alpha via the activation of MAP kinases signal pathways.
Yuan Q, Yang H, Cheng C, Li C, Wu X, Huan W, Sun H, Zhou Z, Wang Y, Zhao Y, Lu X, Shen A
Molecular and cellular biochemistry365(1-2)149-58(2012 Jun)

[Regulation of human ?-1,4-galactosyltransferase V gene expression in cancer cells].
Sato T, Furukawa K
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan132(6)691-7(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta-1,4-GalT-I may be an inflammatory mediator regulating adhesion & migration of inflammatory cells in EAE by modifying galactosylated carbohydrate chains to modulate selectin-ligand biosynthesis & interaction with E-selectin.
Title: Upregulation of ?-1,4-galactosyltransferase I in rat spinal cord with experimental autoimmune encephalomyelitis.

These findings indicated that the functional interaction between CDK11(p58) and beta-1,4-GalT-I may play an important role during astrocyte activation after LPS administration.
Title: The functional interaction between CDK11p58 and ?-1,4-galactosyltransferase I involved in astrocyte activation caused by lipopolysaccharide.

B4galt1 may play an important role in Schwann cell proliferation and apoptosis induced by tumor necrosis factor-alpha via different signal pathways and tumor necrosis factor receptors.
Title: ?-1,4-Galactosyltransferase I involved in Schwann cells proliferation and apoptosis induced by tumor necrosis factor-alpha via the activation of MAP kinases signal pathways.

The expression of the beta-1,4-GalT V gene has been shown to be regulated by transcription factors Sp1 and Ets-1 in cancer cells.(review)
Title: [Regulation of human ?-1,4-galactosyltransferase V gene expression in cancer cells].

Data show that SSeCKS can regulate LPS-induced TNF-alpha secretion through beta-1,4-GalT-I in rat primary astrocytes.
Title: The relationship between Src-suppressed C kinase substrate and ?-1,4 galactosyltransferase-I in the process of lipopolysaccharide-induced TNF-? secretion in rat primary astrocytes.

The following B4galt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B4galt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09183	NM_053287.1 Latest version!	Rattus norvegicus beta-1,4-galactosyltransferase 1 (B4galt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B4galt1 ( NM_053287.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445739.1 Rattus norvegicus beta-1,4-galactosyltransferase 1 (B4galt1), mRNA.

Price & Availability↑

CloneID	ORa09183
Clone ID Related Accession (Same CDS sequence)	NM_053287.1
Accession Version	NM_053287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-galactosyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA818683.1, AA956551.1, AI709691.1, CB741712.1, CB736416.1 and CK364907.1. On or before Oct 11, 2007 this sequence version replaced XM_342820.3, XM_001058948.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTTTC GTGAGCCGTT CCTGGGCGGC AGCGCCGCGA TGCCGGGCGC GACCCTGCAG 
CGGGCCTGCC GCCTGCTCGT GGCGGTCTGC GCGCTGCACC TTGGCGTCAC CCTGGTCTAT 
TACCTCTCCG GTCGCGATCT GAGCCGCCTG CCCCAACTGG TCGGAGTCTC CTCTTCACTG 
CAAGGCGGCA CGAACGGCGC CGCCGCCAGC AAGCAGCCCT CGGGAGAGCT CCGGCCCCGG 
GGCGCGCGGC CGCCGCCTCC TTTAGGCGTC TCCCCGAAGC CTCGCCCGGG TTCTGACTCC 
AGCCCTGATG CGGCTTCTGG CCCCGGCCTG AAGAGCAACT TGACTTCGGT GCCAATGCCC 
ACCAGCACTG GATTGTTGAC TCTGCCTGCT TGCCCTGAGG AGTCCCCGCT GCTCGTTGGC 
CCCATGGTGA TTGACTTTAA TATTCCTGTG GATCTGGAGC TTTTGGCAAA GAAGAACCCA 
GAGATAAAGA TGGGCGGCCG TTACTTCCCC AAGGACTGTA TCTCCCCTCA CAAGGTGGCC 
ATCATTATCC CATTCCGTAA CCGGCAGGAG CACCTCAAAT ACTGGCTGTA TTATTTGCAT 
CCAGTCCTTC AGCGCCAGCA ACTCGACTAT GGCATCTACG TCATCAATCA GGCTGGAGAC 
ACCATGTTTA ATCGAGCTAA GCTGCTCAAC GTTGGCTTTC AAGAGGCCTT GAAAGACTAT 
GACTACAACT GCTTTGTGTT CAGTGATGTG GACCTCATTC CAATGGATGA CCATAATGCC 
TACAGGTGCT TTTCACAGCC ACGGCATATT TCTGTCGCAA TGGACAAGTT CGGGTTTAGC 
CTGCCTTACG TTCAGTATTT TGGAGGTGTC TCCGCTCTCA GTAAACAACA GTTCCTTACC 
ATCAATGGAT TTCCTAATAA TTACTGGGGC TGGGGAGGAG AAGATGATGA CATTTTTAAC 
AGATTAGTTC ATAAAGGCAT GTCTATATCA CGCCCAAATG CTGTGGTAGG CAGGTGTCGC 
ATGATCCGGC ACTCAAGAGA CAAGAAAAAT GAGCCCAACC CTCAGAGGTT TGACCGGATC 
GCACATACAA AGGAAACGAT GCGCCTTGAT GGTTTGAACT CACTTACCTA CCAGGTGTTG 
GACATACAGA GATACCCGTT ATATACCAAA ATCACAGTGG ACATCGGGAC ACCAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445739.1
CDS	219..1418
Translation	MRFREPFLGGSAAMPGATLQRACRLLVAVCALHLGVTLVYYLSGRDLSRLPQLVGVSSSLQGGTNGAAASKQPSGELRPRGARPPPPLGVSPKPRPGSDSSPDAASGPGLKSNLTSVPMPTSTGLLTLPACPEESPLLVGPMVIDFNIPVDLELLAKKNPEIKMGGRYFPKDCISPHKVAIIIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTMFNRAKLLNVGFQEALKDYDYNCFVFSDVDLIPMDDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTINGFPNNYWGWGGEDDDIFNRLVHKGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIAHTKETMRLDGLNSLTYQVLDIQRYPLYTKITVDIGTPR

Target ORF information:

RefSeq Version	NM_053287.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-galactosyltransferase 1 (B4galt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053287.1

ORF Insert Sequence:

ATGAGGTTTC GTGAGCCGTT CCTGGGCGGC AGCGCCGCGA TGCCGGGCGC GACCCTGCAG 
CGGGCCTGCC GCCTGCTCGT GGCGGTCTGC GCGCTGCACC TTGGCGTCAC CCTGGTCTAT 
TACCTCTCCG GTCGCGATCT GAGCCGCCTG CCCCAACTGG TCGGAGTCTC CTCTTCACTG 
CAAGGCGGCA CGAACGGCGC CGCCGCCAGC AAGCAGCCCT CGGGAGAGCT CCGGCCCCGG 
GGCGCGCGGC CGCCGCCTCC TTTAGGCGTC TCCCCGAAGC CTCGCCCGGG TTCTGACTCC 
AGCCCTGATG CGGCTTCTGG CCCCGGCCTG AAGAGCAACT TGACTTCGGT GCCAATGCCC 
ACCAGCACTG GATTGTTGAC TCTGCCTGCT TGCCCTGAGG AGTCCCCGCT GCTCGTTGGC 
CCCATGGTGA TTGACTTTAA TATTCCTGTG GATCTGGAGC TTTTGGCAAA GAAGAACCCA 
GAGATAAAGA TGGGCGGCCG TTACTTCCCC AAGGACTGTA TCTCCCCTCA CAAGGTGGCC 
ATCATTATCC CATTCCGTAA CCGGCAGGAG CACCTCAAAT ACTGGCTGTA TTATTTGCAT 
CCAGTCCTTC AGCGCCAGCA ACTCGACTAT GGCATCTACG TCATCAATCA GGCTGGAGAC 
ACCATGTTTA ATCGAGCTAA GCTGCTCAAC GTTGGCTTTC AAGAGGCCTT GAAAGACTAT 
GACTACAACT GCTTTGTGTT CAGTGATGTG GACCTCATTC CAATGGATGA CCATAATGCC 
TACAGGTGCT TTTCACAGCC ACGGCATATT TCTGTCGCAA TGGACAAGTT CGGGTTTAGC 
CTGCCTTACG TTCAGTATTT TGGAGGTGTC TCCGCTCTCA GTAAACAACA GTTCCTTACC 
ATCAATGGAT TTCCTAATAA TTACTGGGGC TGGGGAGGAG AAGATGATGA CATTTTTAAC 
AGATTAGTTC ATAAAGGCAT GTCTATATCA CGCCCAAATG CTGTGGTAGG CAGGTGTCGC 
ATGATCCGGC ACTCAAGAGA CAAGAAAAAT GAGCCCAACC CTCAGAGGTT TGACCGGATC 
GCACATACAA AGGAAACGAT GCGCCTTGAT GGTTTGAACT CACTTACCTA CCAGGTGTTG 
GACATACAGA GATACCCGTT ATATACCAAA ATCACAGTGG ACATCGGGAC ACCAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Upregulation of ?-1,4-galactosyltransferase I in rat spinal cord with experimental autoimmune encephalomyelitis.
Journal of molecular neuroscience : MN49(3)437-45(2013 Mar)
Zhao J,Gao Y,Cheng C,Yan M,Wang J

The functional interaction between CDK11p58 and ?-1,4-galactosyltransferase I involved in astrocyte activation caused by lipopolysaccharide.
Inflammation35(4)1365-77(2012 Aug)
Liu X,Cheng C,Shao B,Wu X,Ji Y,Lu X,Shen A

?-1,4-Galactosyltransferase I involved in Schwann cells proliferation and apoptosis induced by tumor necrosis factor-alpha via the activation of MAP kinases signal pathways.
Molecular and cellular biochemistry365(1-2)149-58(2012 Jun)
Yuan Q,Yang H,Cheng C,Li C,Wu X,Huan W,Sun H,Zhou Z,Wang Y,Zhao Y,Lu X,Shen A

[Regulation of human ?-1,4-galactosyltransferase V gene expression in cancer cells].
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan132(6)691-7(2012)
Sato T,Furukawa K