Cela1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cela1
Entrez Gene ID	24331
Full Name	chymotrypsin-like elastase family, member 1
Synonyms	ELAI1,Ela1,RATELAI1
General protein information	Preferred Names chymotrypsin-like elastase family, member 1 Names chymotrypsin-like elastase family member 1 elastase 1, pancreatic elastase-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	catalyzes the hydrolysis of elastin and other proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012552.4	NP_036684.1	chymotrypsin-like elastase family member 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CELA1	NP_001962.3
Canis lupus familiaris (dog)	CELA1	NP_001003007.1
Rattus norvegicus (Norway rat)	Cela1	NP_036684.1
Xenopus tropicalis (tropical clawed frog)	LOC496972	NP_001011481.1
Xenopus tropicalis (tropical clawed frog)	ela1	NP_001025669.1
	LOC695507	XP_001088682.1
Xenopus tropicalis (tropical clawed frog)	cela1	NP_001011493.1
Xenopus tropicalis (tropical clawed frog)	LOC101730694	XP_004911959.1
Xenopus tropicalis (tropical clawed frog)	cela1	XP_002933738.2
Pan troglodytes (chimpanzee)	CELA1	XP_001142473.2
Mus musculus (house mouse)	Cela1	NP_291090.2
Xenopus tropicalis (tropical clawed frog)	LOC100135144	XP_002933737.2
Bos taurus (cattle)	CELA1	NP_776473.1
Gallus gallus (chicken)	CELA1	NP_001096672.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
Liu S, Young SM, Varisco BM
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)

Wnt/beta-catenin signaling is required for development of the exocrine pancreas.
Wells JM, Esni F, Boivin GP, Aronow BJ, Stuart W, Combs C, Sklenka A, Leach SD, Lowy AM
BMC developmental biology74(2007 Jan)

Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes.
Hinojos CA, Boerwinkle E, Fornage M, Doris PA
Hypertension (Dallas, Tex. : 1979)45(4)698-704(2005 Apr)

Electron-histochemical location of cathepsin D and elastase in the uterus.
Ryvnyak VV, Gudumak VS, Dulgieru OF
Bulletin of experimental biology and medicine136(2)199-201(2003 Aug)

Elastase involvement in extracellular and intracellular collagen degradation during postpartum involution of the uterus.
Ryvnyak VV, Dulgieru OF
Bulletin of experimental biology and medicine136(2)206-8(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that both cathepsin D and elastase are present in lysosomes of smooth-muscle cells, macrophages, and fibroblasts of the myometrium.
Title: Electron-histochemical location of cathepsin D and elastase in the uterus.

Results describe the location of elastase in rat uterus during its postpartum involution.
Title: Elastase involvement in extracellular and intracellular collagen degradation during postpartum involution of the uterus.

The following Cela1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cela1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12379	NM_012552.4 Latest version!	Rattus norvegicus chymotrypsin like elastase 1 (Cela1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cela1 ( NM_012552.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036684.1 Rattus norvegicus chymotrypsin like elastase 1 (Cela1), mRNA.

Price & Availability↑

CloneID	ORa12379
Clone ID Related Accession (Same CDS sequence)	NM_012552.4
Accession Version	NM_012552.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	chymotrypsin-like elastase family member 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07058908.1 and AABR07058907.1. On Apr 13, 2016 this sequence version replaced NM_012552.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ227752.1, V01234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCGCT TCCTGGTGTT CGCTTCCCTG GTCCTGTATG GACACAGTAC CCAGGACTTT 
CCGGAAACTA ATGCCCGCGT GGTTGGAGGG GCTGAAGCCC GGAGGAACTC CTGGCCATCT 
CAGATTTCCC TCCAGTACCT GTCCGGAGGA TCATGGTACC ACACCTGTGG AGGGACCCTC 
ATCCGACGCA ACTGGGTGAT GACCGCTGCC CACTGTGTGA GCAGCCAGAT GACTTTCCGA 
GTGGTCGTCG GAGACCACAA CCTGAGTCAG AATGACGGCA CGGAGCAGTA CGTGAGCGTG 
CAGAAGATCG TGGTGCACCC CAACTGGAAC AGCAACAATG TGGCTGCAGG CTATGACATC 
GCCCTATTGC GCTTGGCCCA GAGTGTCACA CTCAATAACT ACGTCCAGCT GGCTGTTTTG 
CCCCAGGAGG GAACCATCCT GGCTAACAAC AATCCCTGCT ATATCACAGG CTGGGGGAGA 
ACCAGAACCA ATGGGCAGCT GTCTCAGACC CTGCAGCAGG CGTACCTGCC CAGCGTGGAC 
TACAGCATCT GCTCCAGCTC CTCTTACTGG GGCTCCACGG TGAAGACGAC CATGGTGTGT 
GCTGGTGGAG ACGGAGTCCG CTCTGGGTGC CAGGGTGATT CTGGGGGACC CCTCCATTGC 
TTGGTGAACG GCCAGTATTC TGTCCACGGA GTGACCAGCT TTGTGTCCAG TATGGGCTGT 
AATGTCTCCA GGAAGCCCAC AGTCTTCACC CGAGTCTCTG CTTACATTTC CTGGATGAAC 
AATGTCATTG CCTACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036684.1
CDS	23..823
Translation	MLRFLVFASLVLYGHSTQDFPETNARVVGGAEARRNSWPSQISLQYLSGGSWYHTCGGTLIRRNWVMTAAHCVSSQMTFRVVVGDHNLSQNDGTEQYVSVQKIVVHPNWNSNNVAAGYDIALLRLAQSVTLNNYVQLAVLPQEGTILANNNPCYITGWGRTRTNGQLSQTLQQAYLPSVDYSICSSSSYWGSTVKTTMVCAGGDGVRSGCQGDSGGPLHCLVNGQYSVHGVTSFVSSMGCNVSRKPTVFTRVSAYISWMNNVIAYN

Target ORF information:

RefSeq Version	NM_012552.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chymotrypsin like elastase 1 (Cela1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012552.4

ORF Insert Sequence:

ATGCTGCGCT TCCTGGTGTT CGCTTCCCTG GTCCTGTATG GACACAGTAC CCAGGACTTT 
CCGGAAACTA ATGCCCGCGT GGTTGGAGGG GCTGAAGCCC GGAGGAACTC CTGGCCATCT 
CAGATTTCCC TCCAGTACCT GTCCGGAGGA TCATGGTACC ACACCTGTGG AGGGACCCTC 
ATCCGACGCA ACTGGGTGAT GACCGCTGCC CACTGTGTGA GCAGCCAGAT GACTTTCCGA 
GTGGTCGTCG GAGACCACAA CCTGAGTCAG AATGACGGCA CGGAGCAGTA CGTGAGCGTG 
CAGAAGATCG TGGTGCACCC CAACTGGAAC AGCAACAATG TGGCTGCAGG CTATGACATC 
GCCCTATTGC GCTTGGCCCA GAGTGTCACA CTCAATAACT ACGTCCAGCT GGCTGTTTTG 
CCCCAGGAGG GAACCATCCT GGCTAACAAC AATCCCTGCT ATATCACAGG CTGGGGGAGA 
ACCAGAACCA ATGGGCAGCT GTCTCAGACC CTGCAGCAGG CGTACCTGCC CAGCGTGGAC 
TACAGCATCT GCTCCAGCTC CTCTTACTGG GGCTCCACGG TGAAGACGAC CATGGTGTGT 
GCTGGTGGAG ACGGAGTCCG CTCTGGGTGC CAGGGTGATT CTGGGGGACC CCTCCATTGC 
TTGGTGAACG GCCAGTATTC TGTCCACGGA GTGACCAGCT TTGTGTCCAG TATGGGCTGT 
AATGTCTCCA GGAAGCCCAC AGTCTTCACC CGAGTCTCTG CTTACATTTC CTGGATGAAC 
AATGTCATTG CCTACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)
Liu S,Young SM,Varisco BM

Wnt/beta-catenin signaling is required for development of the exocrine pancreas.
BMC developmental biology74(2007 Jan)
Wells JM,Esni F,Boivin GP,Aronow BJ,Stuart W,Combs C,Sklenka A,Leach SD,Lowy AM

Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes.
Hypertension (Dallas, Tex. : 1979)45(4)698-704(2005 Apr)
Hinojos CA,Boerwinkle E,Fornage M,Doris PA

Electron-histochemical location of cathepsin D and elastase in the uterus.
Bulletin of experimental biology and medicine136(2)199-201(2003 Aug)
Ryvnyak VV,Gudumak VS,Dulgieru OF

Elastase involvement in extracellular and intracellular collagen degradation during postpartum involution of the uterus.
Bulletin of experimental biology and medicine136(2)206-8(2003 Aug)
Ryvnyak VV,Dulgieru OF