Ascl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ascl2
Entrez Gene ID	24209
Full Name	achaete-scute family bHLH transcription factor 2
General protein information	Preferred Names achaete-scute family bHLH transcription factor 2 Names achaete-scute homolog 2 achaete-scute complex homolog 2 achaete-scute complex homolog-like 2 mash2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	may play a role in neural crest cell development and neuronal determination [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031503.2	NP_113691.1	achaete-scute homolog 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ascl2	NP_113691.1
Gallus gallus (chicken)	ASCL2	XP_003641403.1
Pan troglodytes (chimpanzee)	ASCL2	XP_521719.2
Canis lupus familiaris (dog)	ASCL2	XP_005631403.1
Mus musculus (house mouse)	Ascl2	NP_032580.2
Homo sapiens (human)	ASCL2	NP_005161.1
	LOC721175	XP_001117240.2
Bos taurus (cattle)	ASCL2	NP_001035697.1

Gene Ontology
Bibliography

Related articles in PubMed

Atad3 function is essential for early post-implantation development in the mouse.
Goller T, Seibold UK, Kremmer E, Voos W, Kolanus W
PloS one8(1)e54799(2013)

Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction.
Oh-McGinnis R, Bogutz AB, Lefebvre L
Developmental biology351(2)277-86(2011 Mar)

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS
Cell stem cell5(4)420-33(2009 Oct)

Transcription factor achaete scute-like 2 controls intestinal stem cell fate.
van der Flier LG, van Gijn ME, Hatzis P, Kujala P, Haegebarth A, Stange DE, Begthel H, van den Born M, Guryev V, Oving I, van Es JH, Barker N, Peters PJ, van de Wetering M, Clevers H
Cell136(5)903-12(2009 Mar)

The expression of proprotein convertase PACE4 is highly regulated by Hash-2 in placenta: possible role of placenta-specific basic helix-loop-helix transcription factor, human achaete-scute homologue-2.
Koide S, Yoshida I, Tsuji A, Matsuda Y
Journal of biochemistry134(3)433-40(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ascl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ascl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13428	NM_031503.2 Latest version!	Rattus norvegicus achaete-scute family bHLH transcription factor 2 (Ascl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ascl2 ( NM_031503.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113691.1 Rattus norvegicus achaete-scute family bHLH transcription factor 2 (Ascl2), mRNA.

Price & Availability↑

CloneID	ORa13428
Clone ID Related Accession (Same CDS sequence)	NM_031503.2
Accession Version	NM_031503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	achaete-scute homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095135.11. On May 15, 2014 this sequence version replaced NM_031503.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906350 [ECO:0000348] ##Evidence-Data-END##

ATGGAATCGC ACTTTAACTG GTACGGGGTC CCAAGGCTCC AGAAGGCTAG CGACGCGTGC 
CCTAGGGAAT CCTGCAGCAG TGCCCTGCCT GAGGCCCGTG AAGGTGCGAA CGTCCACTTC 
CCACCGCACC CGGTTCCTCG CGAGCACTTT TCCTGTGGCG CACCGAAACC CGTAGCGGGG 
GCCCCGGCGC TGAATGCAAG CTTGATGGAC GGCGGCGCGC TGCCCAGACT CGTGCCCACC 
TCGTCTGGAG TCGCTGGAGC CTGCACTGCT CGGCGGAGAC CCCCGTCCCC GGAACTGCTT 
CGCTGCAGCC GACGGCGGCG ATCGGGAGCA ACGGAGGCCA GCAGCAGCTC GGCGGCCGTG 
GCACGCCGCA ATGAGCGTGA GCGCAACCGC GTAAAGCTGG TAAACTTGGG CTTCCAGGCG 
CTGCGGCAGC ACGTGCCGCA CGGCGGCGCC AACAAGAAGC TGAGTAAGGT GGAGACGCTG 
CGCTCCGCGG TAGAGTACAT CCGTGCGCTG CAGCGGCTGC TAGCAGAGCA CGACGCGGTG 
CGTGCTGCGC TCTCTGGGGG TCTATTAACA CCCGCTACTC GGCCGTCCGA TGTGTGCACG 
CAGCCCTCCG CCTCCCCTGC CAGCGCGTCT CTGTCCTGCA CCTCTACATC CCCAGACCGC 
CTAGGCTGCT CCGAGCCTGC CTCTCCGCGC TCCGCCTACT CGTCGGAGGA CAGCAGCTGC 
GAGGGAGAGA CTTACCCGAT GGGGCAGATG TTTGACTTTT CCAATTGGTT AGGGGGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113691.1
CDS	261..1043
Translation	MESHFNWYGVPRLQKASDACPRESCSSALPEAREGANVHFPPHPVPREHFSCGAPKPVAGAPALNASLMDGGALPRLVPTSSGVAGACTARRRPPSPELLRCSRRRRSGATEASSSSAAVARRNERERNRVKLVNLGFQALRQHVPHGGANKKLSKVETLRSAVEYIRALQRLLAEHDAVRAALSGGLLTPATRPSDVCTQPSASPASASLSCTSTSPDRLGCSEPASPRSAYSSEDSSCEGETYPMGQMFDFSNWLGGY

Target ORF information:

RefSeq Version	NM_031503.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus achaete-scute family bHLH transcription factor 2 (Ascl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031503.2

ORF Insert Sequence:

ATGGAATCGC ACTTTAACTG GTACGGGGTC CCAAGGCTCC AGAAGGCTAG CGACGCGTGC 
CCTAGGGAAT CCTGCAGCAG TGCCCTGCCT GAGGCCCGTG AAGGTGCGAA CGTCCACTTC 
CCACCGCACC CGGTTCCTCG CGAGCACTTT TCCTGTGGCG CACCGAAACC CGTAGCGGGG 
GCCCCGGCGC TGAATGCAAG CTTGATGGAC GGCGGCGCGC TGCCCAGACT CGTGCCCACC 
TCGTCTGGAG TCGCTGGAGC CTGCACTGCT CGGCGGAGAC CCCCGTCCCC GGAACTGCTT 
CGCTGCAGCC GACGGCGGCG ATCGGGAGCA ACGGAGGCCA GCAGCAGCTC GGCGGCCGTG 
GCACGCCGCA ATGAGCGTGA GCGCAACCGC GTAAAGCTGG TAAACTTGGG CTTCCAGGCG 
CTGCGGCAGC ACGTGCCGCA CGGCGGCGCC AACAAGAAGC TGAGTAAGGT GGAGACGCTG 
CGCTCCGCGG TAGAGTACAT CCGTGCGCTG CAGCGGCTGC TAGCAGAGCA CGACGCGGTG 
CGTGCTGCGC TCTCTGGGGG TCTATTAACA CCCGCTACTC GGCCGTCCGA TGTGTGCACG 
CAGCCCTCCG CCTCCCCTGC CAGCGCGTCT CTGTCCTGCA CCTCTACATC CCCAGACCGC 
CTAGGCTGCT CCGAGCCTGC CTCTCCGCGC TCCGCCTACT CGTCGGAGGA CAGCAGCTGC 
GAGGGAGAGA CTTACCCGAT GGGGCAGATG TTTGACTTTT CCAATTGGTT AGGGGGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Atad3 function is essential for early post-implantation development in the mouse.
PloS one8(1)e54799(2013)
Goller T,Seibold UK,Kremmer E,Voos W,Kolanus W

Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction.
Developmental biology351(2)277-86(2011 Mar)
Oh-McGinnis R,Bogutz AB,Lefebvre L

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Cell stem cell5(4)420-33(2009 Oct)
Nishiyama A,Xin L,Sharov AA,Thomas M,Mowrer G,Meyers E,Piao Y,Mehta S,Yee S,Nakatake Y,Stagg C,Sharova L,Correa-Cerro LS,Bassey U,Hoang H,Kim E,Tapnio R,Qian Y,Dudekula D,Zalzman M,Li M,Falco G,Yang HT,Lee SL,Monti M,Stanghellini I,Islam MN,Nagaraja R,Goldberg I,Wang W,Longo DL,Schlessinger D,Ko MS

Transcription factor achaete scute-like 2 controls intestinal stem cell fate.
Cell136(5)903-12(2009 Mar)
van der Flier LG,van Gijn ME,Hatzis P,Kujala P,Haegebarth A,Stange DE,Begthel H,van den Born M,Guryev V,Oving I,van Es JH,Barker N,Peters PJ,van de Wetering M,Clevers H

The expression of proprotein convertase PACE4 is highly regulated by Hash-2 in placenta: possible role of placenta-specific basic helix-loop-helix transcription factor, human achaete-scute homologue-2.
Journal of biochemistry134(3)433-40(2003 Sep)
Koide S,Yoshida I,Tsuji A,Matsuda Y