H1f4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1f4
Entrez Gene ID	201097
Full Name	histone cluster 1, H1d
Synonyms	H14,H1d,H1f4,Hist1h1c,Hist1h1e
General protein information	Preferred Names histone cluster 1, H1d Names histone H1.4 H1 histone family, member 4 Histone 1d histone H1.2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p11
Summary	facilitates the organization and condensation of polynucleosome filaments into chromatin fibers [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133285.1	NP_579819.1	histone H1.4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hist1h1d	NP_579819.1
Mus musculus (house mouse)	Hist1h1e	NP_056602.1

Gene Ontology
Bibliography

Related articles in PubMed

Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency.
Hayakawa K, Ohgane J, Tanaka S, Yagi S, Shiota K
Epigenetics7(9)1029-36(2012 Sep)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Histone H1 recruitment by CHD8 is essential for suppression of the Wnt-?-catenin signaling pathway.
Nishiyama M, Skoultchi AI, Nakayama KI
Molecular and cellular biology32(2)501-12(2012 Jan)

Reduction of Hox gene expression by histone H1 depletion.
Zhang Y, Liu Z, Medrzycki M, Cao K, Fan Y
PloS one7(6)e38829(2012)

Chromatin supraorganization, mitotic abnormalities and proliferation in cells with increased or down-regulated lox expression: Indirect evidence of a LOX-histone H1 interaction in vivo.
Mello ML, Alvarenga EM, Vidal Bde C, Di Donato A
Micron (Oxford, England : 1993)42(1)8-16(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data presented herein regarding chromatin remodeling indirectly support the hypothesis that LOX binds to histone H1 in vivo.
Title: Chromatin supraorganization, mitotic abnormalities and proliferation in cells with increased or down-regulated lox expression: Indirect evidence of a LOX-histone H1 interaction in vivo.

Prediction of an HMG-box fold in the C-terminal domain of histone H1d: insights into its role in DNA condensation
Title: Prediction of an HMG-box fold in the C-terminal domain of histone H1: insights into its role in DNA condensation.

A 34 amino acid stretch (residues 184-218) in the C-terminus of histone H1d has been identified as the DNA-condensing domain; when entirely deleted, there is nearly a 90% decrease in DNA condensation.
Title: Identification of a 34 amino acid stretch within the C-terminus of histone H1 as the DNA-condensing domain by site-directed mutagenesis.

The following H1f4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1f4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42663	NM_133285.1 Latest version!	Rattus norvegicus H1.4 linker histone, cluster member (H1f4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1f4 ( NM_133285.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_579819.1 Rattus norvegicus H1.4 linker histone, cluster member (H1f4), mRNA.

Price & Availability↑

CloneID	ORa42663
Clone ID Related Accession (Same CDS sequence)	NM_133285.1
Accession Version	NM_133285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	histone H1.4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X67320.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTCTGAGA CTGCCCCTGC TGCGCCCGCC GCACCGGCCC CCGCAGAGAA GACACCCATA 
AAGAAGAAGG CCCGCAAGGC CGCAGGTGGT GCGAAGCGCA AAGCGTCCGG ACCCCCGGTG 
TCCGAGCTCA TCACCAAGGC TGTGGCCGCC TCCAAGGAGC GCAGCGGCGT GTCTCTGGCC 
GCGCTCAAGA AAGCGCTGGC TGCCGCCGGC TACGATGTGG AGAAGAATAA CAGCCGCATC 
AAGCTCGGGC TGAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCCTCCGGCT CCTTCAAACT CAACAAGAAG GCGGCTTCCG GCGAAGCCAA GCCCAAAGCA 
AAGAAGGCAG GCGCGGCTAA GGCGAAGAAG CCTGCAGGCG CTGCCAAGAA GCCCAAGAAG 
GCTACGGGAA CAGCCACTCC CAAGAAGAGC ACTAAGAAGA CTCCAAAGAA AGCAAAGAAG 
CCGGCTGCAG CTGCAGGAGC CAAAAAAGCT AAAAGCCCGA AAAAAGCAAA AGCTACTAAG 
GCTAAGAAGG CCCCCAAGAG CCCTGCCAAG GCGAGAGCGG TAAAGCCCAA AGCGGCCAAA 
CCAAAAACCT CCAAGCCTAA AGCAGCTAAG CCAAAGAAAA CAGCAGCCAA GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579819.1
CDS	1..660
Translation	MSETAPAAPAAPAPAEKTPIKKKARKAAGGAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPKKATGTATPKKSTKKTPKKAKKPAAAAGAKKAKSPKKAKATKAKKAPKSPAKARAVKPKAAKPKTSKPKAAKPKKTAAKKK

Target ORF information:

RefSeq Version	NM_133285.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus H1.4 linker histone, cluster member (H1f4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133285.1

ORF Insert Sequence:

ATGTCTGAGA CTGCCCCTGC TGCGCCCGCC GCACCGGCCC CCGCAGAGAA GACACCCATA 
AAGAAGAAGG CCCGCAAGGC CGCAGGTGGT GCGAAGCGCA AAGCGTCCGG ACCCCCGGTG 
TCCGAGCTCA TCACCAAGGC TGTGGCCGCC TCCAAGGAGC GCAGCGGCGT GTCTCTGGCC 
GCGCTCAAGA AAGCGCTGGC TGCCGCCGGC TACGATGTGG AGAAGAATAA CAGCCGCATC 
AAGCTCGGGC TGAAGAGCCT GGTGAGCAAG GGCACCCTGG TGCAGACCAA GGGCACCGGC 
GCCTCCGGCT CCTTCAAACT CAACAAGAAG GCGGCTTCCG GCGAAGCCAA GCCCAAAGCA 
AAGAAGGCAG GCGCGGCTAA GGCGAAGAAG CCTGCAGGCG CTGCCAAGAA GCCCAAGAAG 
GCTACGGGAA CAGCCACTCC CAAGAAGAGC ACTAAGAAGA CTCCAAAGAA AGCAAAGAAG 
CCGGCTGCAG CTGCAGGAGC CAAAAAAGCT AAAAGCCCGA AAAAAGCAAA AGCTACTAAG 
GCTAAGAAGG CCCCCAAGAG CCCTGCCAAG GCGAGAGCGG TAAAGCCCAA AGCGGCCAAA 
CCAAAAACCT CCAAGCCTAA AGCAGCTAAG CCAAAGAAAA CAGCAGCCAA GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency.
Epigenetics7(9)1029-36(2012 Sep)
Hayakawa K,Ohgane J,Tanaka S,Yagi S,Shiota K

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Histone H1 recruitment by CHD8 is essential for suppression of the Wnt-?-catenin signaling pathway.
Molecular and cellular biology32(2)501-12(2012 Jan)
Nishiyama M,Skoultchi AI,Nakayama KI

Reduction of Hox gene expression by histone H1 depletion.
PloS one7(6)e38829(2012)
Zhang Y,Liu Z,Medrzycki M,Cao K,Fan Y

Chromatin supraorganization, mitotic abnormalities and proliferation in cells with increased or down-regulated lox expression: Indirect evidence of a LOX-histone H1 interaction in vivo.
Micron (Oxford, England : 1993)42(1)8-16(2011 Jan)
Mello ML,Alvarenga EM,Vidal Bde C,Di Donato A