Gls2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gls2
Entrez Gene ID	192268
Full Name	glutaminase 2
Synonyms	Ga
General protein information	Preferred Names glutaminase 2 Names glutaminase liver isoform, mitochondrial GLS L-glutaminase L-glutamine amidohydrolase glutaminase 2 (liver, mitochondrial) glutaminase variant 2 glutaminase variant 3 glutaminase variant 4 glutaminase variant 5 glutaminase variant 6 glutaminase variant 8 liver mitochondrial glutaminase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	first enzyme of glutamine catabolism in liver [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594653.1	XP_017450142.1	glutaminase liver isoform, mitochondrial isoform X3
NM_138904.2	NP_620259.2	glutaminase liver isoform, mitochondrial isoform 3
XM_006240728.3	XP_006240790.1	glutaminase liver isoform, mitochondrial isoform X1
XM_017594654.1	XP_017450143.1	glutaminase liver isoform, mitochondrial isoform X5
NM_001270786.1	NP_001257715.1	glutaminase liver isoform, mitochondrial isoform 1
XM_017594652.1	XP_017450141.1	glutaminase liver isoform, mitochondrial isoform X2
NM_001270787.1	NP_001257716.1	glutaminase liver isoform, mitochondrial isoform 2
XM_006240730.2	XP_006240792.1	glutaminase liver isoform, mitochondrial isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLS2	NP_037399.2
Pan troglodytes (chimpanzee)	GLS2	XP_509148.2
Rattus norvegicus (Norway rat)	Gls2	NP_001257715.1
Danio rerio (zebrafish)	gls2	XP_001345099.5
Xenopus tropicalis (tropical clawed frog)	gls2	XP_002934555.2
Bos taurus (cattle)	GLS2	NP_001179203.1
	GLS2	XP_001115245.1
Danio rerio (zebrafish)	si:dkey-102c8.6	NP_001077294.1
Canis lupus familiaris (dog)	GLS2	XP_003431516.1
Mus musculus (house mouse)	Gls2	NP_001028436.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Gls and Gls2 glutaminase isoforms in astrocytes.
Cardona C, S?nchez-Mej?as E, D?vila JC, Mart?n-Rufi?n M, Campos-Sandoval JA, Vitorica J, Alonso FJ, Mat?s JM, Segura JA, Norenberg MD, Rama Rao KV, Jayakumar AR, Guti?rrez A, M?rquez J
Glia63(3)365-82(2015 Mar)

Phosphate-dependent glutaminase response to liver injury and hyperbaric oxygenation.
Savilov PN, Yakovlev VN
Bulletin of experimental biology and medicine157(3)299-301(2014 Jul)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.
Mart?n-Rufi?n M, Tosina M, Campos-Sandoval JA, Manzanares E, Lobo C, Segura JA, Alonso FJ, Mat?s JM, M?rquez J
PloS one7(6)e38380(2012)

Phosphate-activated glutaminase (GLS2), a p53-inducible regulator of glutamine metabolism and reactive oxygen species.
Suzuki S, Tanaka T, Poyurovsky MV, Nagano H, Mayama T, Ohkubo S, Lokshin M, Hosokawa H, Nakayama T, Suzuki Y, Sugano S, Sato E, Nagao T, Yokote K, Tatsuno I, Prives C
Proceedings of the National Academy of Sciences of the United States of America107(16)7461-6(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GABAergic neurons and astrocytes express Gls and Gls2 isoenzymes in nucleus and mitochondria, in addition to glutamatergic neurons
Title: Expression of Gls and Gls2 glutaminase isoforms in astrocytes.

In animals with chronic hepatitis; hyperbaric oxygenation restricted the stimulating effect of hepatic resection on phosphate-dependent glutaminase activity.
Title: Phosphate-dependent glutaminase response to liver injury and hyperbaric oxygenation.

Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented.
Title: Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.

The following Gls2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gls2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12172	NM_001270786.1 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa12180	NM_138904.2 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa12324	NM_001270787.1 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa12180	XM_006240730.2 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa29729	XM_006240728.3 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa61893	XM_017594653.1 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa61894	XM_017594654.1 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa61892	XM_017594652.1 Latest version!	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gls2 ( NM_001270786.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257715.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12172
Clone ID Related Accession (Same CDS sequence)	NM_001270786.1
Accession Version	NM_001270786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109891.5 and BC089776.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC089776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CATGATGCCT CGAACAGTGG CATGTTGCCT CGACTTGGTG ACCTGCTTTT CTACACCATT 
GCAGAGGGGC AGGAACGCAT CCCCATCCAT AAGTTCACCA CGGCCCTGAA GGCCACTGGA 
CTGCAGACGT CAGACCCACG GCTCCAGGAC TGCATGAGCA AGATGCAGCG CATGGTCCAA 
GAGTCCAGCA GTGGTGGCCT CTTGGACCGA GAACTCTTCC AAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACTCAGGC ATTCCGGAAG AAGTTTGTCA TTCCTGACTT TGAGGAGTTC 
ACAGGCCATG TGGATCGTAT ATTTGAGGAT GCCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCACCTGGC CAAGTCAAAC CCAGATCTTT GGGGCGTCTC CCTGTGCACT 
GTGGATGGGC AGCGACACTC TGTGGGCCAC ACGAAGATCC CTTTCTGCCT ACAGTCCTGT 
GTCAAGCCAC TCACTTACGC CATCTCCGTG AGCACCTTAG GCACCGACTA CGTGCACAAG 
TTCGTGGGCA AGGAACCCAG TGGTCTGCGC TATAACAAAC TCTCCCTCAA CGAGGAAGGA 
ATCCCTCATA ACCCCATGGT CAATGCTGGT GCTATTGTGG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGACTTT GTGTTACAGT ATCTGAACAA GATGGCTGGG 
AATGAATTCA TGGGGTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAGAC TGGGGATCGG 
AATTACGCCA TTGGCTATTA CCTCAAGGAG AAGAAGTGCT TCCCTAAGGG AGTGGACATG 
ATGGCTGCCC TTGATCTCTA TTTCCAGCTG TGCTCTGTGG AGGTTACCTG TGAGTCAGGC 
AGTGTCATGG CGGCCACTCT TGCCAATGGC GGCATCTGCC CCATCACAGG GGAGAGCGTG 
CTGAGTGCGG AAGCCGTGCG CAACACCCTC AGCCTCATGC ACTCCTGTGG CATGTATGAC 
TTCTCGGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT GTCGGGAGCC 
ATCCTCCTGG TTGTACCCAA TGTCATGGGG ATGATGTGTC TGTCACCCCC GTTAGACAAG 
CTGGGGAACA GCCACAGGGG CATCAGCTTC TGCCAGAAGT TGGTGTCTCT GTTTAACTTC 
CACAACTACG ACAACCTGCG GCACTGTGCT CGGAAGTTAG ACCCACGGAG GGAAGGGGGG 
GAAGTTCGGA ACAAGACCGT GGTGAACCTG TTATTTGCTG CATATAGTGG AGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCTGCCGTG GATATGGAGC AGAAGGACTA TGATTCCCGC 
ACAGCCCTAC ATGTGGCGGC AGCGGAAGGA CACATTGACG TTGTCAAGTT TCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGTCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCC 
GTGCAGTTCA ATCACCTGGA GGTGGTCAAA CTGCTTCAGG ATTACCATGA CTCCTACATG 
CTGTCTGAGA CTCAAGCTGA GGTAGCAGCT GAGACTCTGT CAAAAGAGAA CTTAGAGAGC 
ATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257715.1
CDS	237..2045
Translation	MRSMRALQNALSRAGSHGQRGGWGHPSRGPLLGGGVRYYFGEAAAQGRGTPHSHQPQHSDHDASNSGMLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	NM_001270786.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270786.1

ORF Insert Sequence:

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CATGATGCCT CGAACAGTGG CATGTTGCCT CGACTTGGTG ACCTGCTTTT CTACACCATT 
GCAGAGGGGC AGGAACGCAT CCCCATCCAT AAGTTCACCA CGGCCCTGAA GGCCACTGGA 
CTGCAGACGT CAGACCCACG GCTCCAGGAC TGCATGAGCA AGATGCAGCG CATGGTCCAA 
GAGTCCAGCA GTGGTGGCCT CTTGGACCGA GAACTCTTCC AAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACTCAGGC ATTCCGGAAG AAGTTTGTCA TTCCTGACTT TGAGGAGTTC 
ACAGGCCATG TGGATCGTAT ATTTGAGGAT GCCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCACCTGGC CAAGTCAAAC CCAGATCTTT GGGGCGTCTC CCTGTGCACT 
GTGGATGGGC AGCGACACTC TGTGGGCCAC ACGAAGATCC CTTTCTGCCT ACAGTCCTGT 
GTCAAGCCAC TCACTTACGC CATCTCCGTG AGCACCTTAG GCACCGACTA CGTGCACAAG 
TTCGTGGGCA AGGAACCCAG TGGTCTGCGC TATAACAAAC TCTCCCTCAA CGAGGAAGGA 
ATCCCTCATA ACCCCATGGT CAATGCTGGT GCTATTGTGG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGACTTT GTGTTACAGT ATCTGAACAA GATGGCTGGG 
AATGAATTCA TGGGGTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAGAC TGGGGATCGG 
AATTACGCCA TTGGCTATTA CCTCAAGGAG AAGAAGTGCT TCCCTAAGGG AGTGGACATG 
ATGGCTGCCC TTGATCTCTA TTTCCAGCTG TGCTCTGTGG AGGTTACCTG TGAGTCAGGC 
AGTGTCATGG CGGCCACTCT TGCCAATGGC GGCATCTGCC CCATCACAGG GGAGAGCGTG 
CTGAGTGCGG AAGCCGTGCG CAACACCCTC AGCCTCATGC ACTCCTGTGG CATGTATGAC 
TTCTCGGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT GTCGGGAGCC 
ATCCTCCTGG TTGTACCCAA TGTCATGGGG ATGATGTGTC TGTCACCCCC GTTAGACAAG 
CTGGGGAACA GCCACAGGGG CATCAGCTTC TGCCAGAAGT TGGTGTCTCT GTTTAACTTC 
CACAACTACG ACAACCTGCG GCACTGTGCT CGGAAGTTAG ACCCACGGAG GGAAGGGGGG 
GAAGTTCGGA ACAAGACCGT GGTGAACCTG TTATTTGCTG CATATAGTGG AGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCTGCCGTG GATATGGAGC AGAAGGACTA TGATTCCCGC 
ACAGCCCTAC ATGTGGCGGC AGCGGAAGGA CACATTGACG TTGTCAAGTT TCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGTCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCC 
GTGCAGTTCA ATCACCTGGA GGTGGTCAAA CTGCTTCAGG ATTACCATGA CTCCTACATG 
CTGTCTGAGA CTCAAGCTGA GGTAGCAGCT GAGACTCTGT CAAAAGAGAA CTTAGAGAGC 
ATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( NM_138904.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620259.2 Rattus norvegicus glutaminase 2 (Gls2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa12180
Clone ID Related Accession (Same CDS sequence)	NM_138904.2 , XM_006240730.2
Accession Version	NM_138904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109891.5 and BC089776.1. On Aug 1, 2012 this sequence version replaced NM_138904.1. Transcript Variant: This variant (3) lacks the first exon and extends 5' into intronic sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: J05499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGCCTC GACTTGGTGA CCTGCTTTTC TACACCATTG CAGAGGGGCA GGAACGCATC 
CCCATCCATA AGTTCACCAC GGCCCTGAAG GCCACTGGAC TGCAGACGTC AGACCCACGG 
CTCCAGGACT GCATGAGCAA GATGCAGCGC ATGGTCCAAG AGTCCAGCAG TGGTGGCCTC 
TTGGACCGAG AACTCTTCCA AAAGTGTGTG AGCAGCAACA TTGTGCTCCT GACTCAGGCA 
TTCCGGAAGA AGTTTGTCAT TCCTGACTTT GAGGAGTTCA CAGGCCATGT GGATCGTATA 
TTTGAGGATG CCAAAGAGCT CACTGGAGGC AAAGTGGCAG CCTACATCCC TCACCTGGCC 
AAGTCAAACC CAGATCTTTG GGGCGTCTCC CTGTGCACTG TGGATGGGCA GCGACACTCT 
GTGGGCCACA CGAAGATCCC TTTCTGCCTA CAGTCCTGTG TCAAGCCACT CACTTACGCC 
ATCTCCGTGA GCACCTTAGG CACCGACTAC GTGCACAAGT TCGTGGGCAA GGAACCCAGT 
GGTCTGCGCT ATAACAAACT CTCCCTCAAC GAGGAAGGAA TCCCTCATAA CCCCATGGTC 
AATGCTGGTG CTATTGTGGT CAGCTCCCTG ATCAAGATGG ACTGTAACAA AGCAGAGAAG 
TTTGACTTTG TGTTACAGTA TCTGAACAAG ATGGCTGGGA ATGAATTCAT GGGGTTCAGC 
AATGCCACAT TCCAGTCAGA GAAGGAGACT GGGGATCGGA ATTACGCCAT TGGCTATTAC 
CTCAAGGAGA AGAAGTGCTT CCCTAAGGGA GTGGACATGA TGGCTGCCCT TGATCTCTAT 
TTCCAGCTGT GCTCTGTGGA GGTTACCTGT GAGTCAGGCA GTGTCATGGC GGCCACTCTT 
GCCAATGGCG GCATCTGCCC CATCACAGGG GAGAGCGTGC TGAGTGCGGA AGCCGTGCGC 
AACACCCTCA GCCTCATGCA CTCCTGTGGC ATGTATGACT TCTCGGGCCA GTTTGCCTTC 
CACGTGGGCC TGCCAGCCAA GTCAGCTGTG TCGGGAGCCA TCCTCCTGGT TGTACCCAAT 
GTCATGGGGA TGATGTGTCT GTCACCCCCG TTAGACAAGC TGGGGAACAG CCACAGGGGC 
ATCAGCTTCT GCCAGAAGTT GGTGTCTCTG TTTAACTTCC ACAACTACGA CAACCTGCGG 
CACTGTGCTC GGAAGTTAGA CCCACGGAGG GAAGGGGGGG AAGTTCGGAA CAAGACCGTG 
GTGAACCTGT TATTTGCTGC ATATAGTGGA GATGTCTCAG CTCTTCGAAG GTTTGCCTTG 
TCTGCCGTGG ATATGGAGCA GAAGGACTAT GATTCCCGCA CAGCCCTACA TGTGGCGGCA 
GCGGAAGGAC ACATTGACGT TGTCAAGTTT CTGATCGAGG CTTGCAAAGT GAATCCTTTT 
GTCAAGGACA GGTGGGGCAA CATTCCCCTG GATGATGCCG TGCAGTTCAA TCACCTGGAG 
GTGGTCAAAC TGCTTCAGGA TTACCATGAC TCCTACATGC TGTCTGAGAC TCAAGCTGAG 
GTAGCAGCTG AGACTCTGTC AAAAGAGAAC TTAGAGAGCA TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620259.2
CDS	133..1740
Translation	MLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	NM_138904.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138904.2

ORF Insert Sequence:

ATGTTGCCTC GACTTGGTGA CCTGCTTTTC TACACCATTG CAGAGGGGCA GGAACGCATC 
CCCATCCATA AGTTCACCAC GGCCCTGAAG GCCACTGGAC TGCAGACGTC AGACCCACGG 
CTCCAGGACT GCATGAGCAA GATGCAGCGC ATGGTCCAAG AGTCCAGCAG TGGTGGCCTC 
TTGGACCGAG AACTCTTCCA AAAGTGTGTG AGCAGCAACA TTGTGCTCCT GACTCAGGCA 
TTCCGGAAGA AGTTTGTCAT TCCTGACTTT GAGGAGTTCA CAGGCCATGT GGATCGTATA 
TTTGAGGATG CCAAAGAGCT CACTGGAGGC AAAGTGGCAG CCTACATCCC TCACCTGGCC 
AAGTCAAACC CAGATCTTTG GGGCGTCTCC CTGTGCACTG TGGATGGGCA GCGACACTCT 
GTGGGCCACA CGAAGATCCC TTTCTGCCTA CAGTCCTGTG TCAAGCCACT CACTTACGCC 
ATCTCCGTGA GCACCTTAGG CACCGACTAC GTGCACAAGT TCGTGGGCAA GGAACCCAGT 
GGTCTGCGCT ATAACAAACT CTCCCTCAAC GAGGAAGGAA TCCCTCATAA CCCCATGGTC 
AATGCTGGTG CTATTGTGGT CAGCTCCCTG ATCAAGATGG ACTGTAACAA AGCAGAGAAG 
TTTGACTTTG TGTTACAGTA TCTGAACAAG ATGGCTGGGA ATGAATTCAT GGGGTTCAGC 
AATGCCACAT TCCAGTCAGA GAAGGAGACT GGGGATCGGA ATTACGCCAT TGGCTATTAC 
CTCAAGGAGA AGAAGTGCTT CCCTAAGGGA GTGGACATGA TGGCTGCCCT TGATCTCTAT 
TTCCAGCTGT GCTCTGTGGA GGTTACCTGT GAGTCAGGCA GTGTCATGGC GGCCACTCTT 
GCCAATGGCG GCATCTGCCC CATCACAGGG GAGAGCGTGC TGAGTGCGGA AGCCGTGCGC 
AACACCCTCA GCCTCATGCA CTCCTGTGGC ATGTATGACT TCTCGGGCCA GTTTGCCTTC 
CACGTGGGCC TGCCAGCCAA GTCAGCTGTG TCGGGAGCCA TCCTCCTGGT TGTACCCAAT 
GTCATGGGGA TGATGTGTCT GTCACCCCCG TTAGACAAGC TGGGGAACAG CCACAGGGGC 
ATCAGCTTCT GCCAGAAGTT GGTGTCTCTG TTTAACTTCC ACAACTACGA CAACCTGCGG 
CACTGTGCTC GGAAGTTAGA CCCACGGAGG GAAGGGGGGG AAGTTCGGAA CAAGACCGTG 
GTGAACCTGT TATTTGCTGC ATATAGTGGA GATGTCTCAG CTCTTCGAAG GTTTGCCTTG 
TCTGCCGTGG ATATGGAGCA GAAGGACTAT GATTCCCGCA CAGCCCTACA TGTGGCGGCA 
GCGGAAGGAC ACATTGACGT TGTCAAGTTT CTGATCGAGG CTTGCAAAGT GAATCCTTTT 
GTCAAGGACA GGTGGGGCAA CATTCCCCTG GATGATGCCG TGCAGTTCAA TCACCTGGAG 
GTGGTCAAAC TGCTTCAGGA TTACCATGAC TCCTACATGC TGTCTGAGAC TCAAGCTGAG 
GTAGCAGCTG AGACTCTGTC AAAAGAGAAC TTAGAGAGCA TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( NM_001270787.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257716.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12324
Clone ID Related Accession (Same CDS sequence)	NM_001270787.1
Accession Version	NM_001270787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109891.5 and BC089776.1. Transcript Variant: This variant (2) uses an alternate splice site at the 3' end of the first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FQ211735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTTTTC CTTTAGATGT GGGGCTTTGG GTCCGCCGCG CTCGGGCGGT CAGGTGCTGT 
CCGCCCGGGA GGGCGTTTGG GCAGGTCGGT CTTCGAGATG ATGCCTGTCC AAGTGATGCC 
TCGAACAGTG GCATGTTGCC TCGACTTGGT GACCTGCTTT TCTACACCAT TGCAGAGGGG 
CAGGAACGCA TCCCCATCCA TAAGTTCACC ACGGCCCTGA AGGCCACTGG ACTGCAGACG 
TCAGACCCAC GGCTCCAGGA CTGCATGAGC AAGATGCAGC GCATGGTCCA AGAGTCCAGC 
AGTGGTGGCC TCTTGGACCG AGAACTCTTC CAAAAGTGTG TGAGCAGCAA CATTGTGCTC 
CTGACTCAGG CATTCCGGAA GAAGTTTGTC ATTCCTGACT TTGAGGAGTT CACAGGCCAT 
GTGGATCGTA TATTTGAGGA TGCCAAAGAG CTCACTGGAG GCAAAGTGGC AGCCTACATC 
CCTCACCTGG CCAAGTCAAA CCCAGATCTT TGGGGCGTCT CCCTGTGCAC TGTGGATGGG 
CAGCGACACT CTGTGGGCCA CACGAAGATC CCTTTCTGCC TACAGTCCTG TGTCAAGCCA 
CTCACTTACG CCATCTCCGT GAGCACCTTA GGCACCGACT ACGTGCACAA GTTCGTGGGC 
AAGGAACCCA GTGGTCTGCG CTATAACAAA CTCTCCCTCA ACGAGGAAGG AATCCCTCAT 
AACCCCATGG TCAATGCTGG TGCTATTGTG GTCAGCTCCC TGATCAAGAT GGACTGTAAC 
AAAGCAGAGA AGTTTGACTT TGTGTTACAG TATCTGAACA AGATGGCTGG GAATGAATTC 
ATGGGGTTCA GCAATGCCAC ATTCCAGTCA GAGAAGGAGA CTGGGGATCG GAATTACGCC 
ATTGGCTATT ACCTCAAGGA GAAGAAGTGC TTCCCTAAGG GAGTGGACAT GATGGCTGCC 
CTTGATCTCT ATTTCCAGCT GTGCTCTGTG GAGGTTACCT GTGAGTCAGG CAGTGTCATG 
GCGGCCACTC TTGCCAATGG CGGCATCTGC CCCATCACAG GGGAGAGCGT GCTGAGTGCG 
GAAGCCGTGC GCAACACCCT CAGCCTCATG CACTCCTGTG GCATGTATGA CTTCTCGGGC 
CAGTTTGCCT TCCACGTGGG CCTGCCAGCC AAGTCAGCTG TGTCGGGAGC CATCCTCCTG 
GTTGTACCCA ATGTCATGGG GATGATGTGT CTGTCACCCC CGTTAGACAA GCTGGGGAAC 
AGCCACAGGG GCATCAGCTT CTGCCAGAAG TTGGTGTCTC TGTTTAACTT CCACAACTAC 
GACAACCTGC GGCACTGTGC TCGGAAGTTA GACCCACGGA GGGAAGGGGG GGAAGTTCGG 
AACAAGACCG TGGTGAACCT GTTATTTGCT GCATATAGTG GAGATGTCTC AGCTCTTCGA 
AGGTTTGCCT TGTCTGCCGT GGATATGGAG CAGAAGGACT ATGATTCCCG CACAGCCCTA 
CATGTGGCGG CAGCGGAAGG ACACATTGAC GTTGTCAAGT TTCTGATCGA GGCTTGCAAA 
GTGAATCCTT TTGTCAAGGA CAGGTGGGGC AACATTCCCC TGGATGATGC CGTGCAGTTC 
AATCACCTGG AGGTGGTCAA ACTGCTTCAG GATTACCATG ACTCCTACAT GCTGTCTGAG 
ACTCAAGCTG AGGTAGCAGC TGAGACTCTG TCAAAAGAGA ACTTAGAGAG CATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257716.1
CDS	552..2291
Translation	MPFPLDVGLWVRRARAVRCCPPGRAFGQVGLRDDACPSDASNSGMLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	NM_001270787.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270787.1

ORF Insert Sequence:

ATGCCTTTTC CTTTAGATGT GGGGCTTTGG GTCCGCCGCG CTCGGGCGGT CAGGTGCTGT 
CCGCCCGGGA GGGCGTTTGG GCAGGTCGGT CTTCGAGATG ATGCCTGTCC AAGTGATGCC 
TCGAACAGTG GCATGTTGCC TCGACTTGGT GACCTGCTTT TCTACACCAT TGCAGAGGGG 
CAGGAACGCA TCCCCATCCA TAAGTTCACC ACGGCCCTGA AGGCCACTGG ACTGCAGACG 
TCAGACCCAC GGCTCCAGGA CTGCATGAGC AAGATGCAGC GCATGGTCCA AGAGTCCAGC 
AGTGGTGGCC TCTTGGACCG AGAACTCTTC CAAAAGTGTG TGAGCAGCAA CATTGTGCTC 
CTGACTCAGG CATTCCGGAA GAAGTTTGTC ATTCCTGACT TTGAGGAGTT CACAGGCCAT 
GTGGATCGTA TATTTGAGGA TGCCAAAGAG CTCACTGGAG GCAAAGTGGC AGCCTACATC 
CCTCACCTGG CCAAGTCAAA CCCAGATCTT TGGGGCGTCT CCCTGTGCAC TGTGGATGGG 
CAGCGACACT CTGTGGGCCA CACGAAGATC CCTTTCTGCC TACAGTCCTG TGTCAAGCCA 
CTCACTTACG CCATCTCCGT GAGCACCTTA GGCACCGACT ACGTGCACAA GTTCGTGGGC 
AAGGAACCCA GTGGTCTGCG CTATAACAAA CTCTCCCTCA ACGAGGAAGG AATCCCTCAT 
AACCCCATGG TCAATGCTGG TGCTATTGTG GTCAGCTCCC TGATCAAGAT GGACTGTAAC 
AAAGCAGAGA AGTTTGACTT TGTGTTACAG TATCTGAACA AGATGGCTGG GAATGAATTC 
ATGGGGTTCA GCAATGCCAC ATTCCAGTCA GAGAAGGAGA CTGGGGATCG GAATTACGCC 
ATTGGCTATT ACCTCAAGGA GAAGAAGTGC TTCCCTAAGG GAGTGGACAT GATGGCTGCC 
CTTGATCTCT ATTTCCAGCT GTGCTCTGTG GAGGTTACCT GTGAGTCAGG CAGTGTCATG 
GCGGCCACTC TTGCCAATGG CGGCATCTGC CCCATCACAG GGGAGAGCGT GCTGAGTGCG 
GAAGCCGTGC GCAACACCCT CAGCCTCATG CACTCCTGTG GCATGTATGA CTTCTCGGGC 
CAGTTTGCCT TCCACGTGGG CCTGCCAGCC AAGTCAGCTG TGTCGGGAGC CATCCTCCTG 
GTTGTACCCA ATGTCATGGG GATGATGTGT CTGTCACCCC CGTTAGACAA GCTGGGGAAC 
AGCCACAGGG GCATCAGCTT CTGCCAGAAG TTGGTGTCTC TGTTTAACTT CCACAACTAC 
GACAACCTGC GGCACTGTGC TCGGAAGTTA GACCCACGGA GGGAAGGGGG GGAAGTTCGG 
AACAAGACCG TGGTGAACCT GTTATTTGCT GCATATAGTG GAGATGTCTC AGCTCTTCGA 
AGGTTTGCCT TGTCTGCCGT GGATATGGAG CAGAAGGACT ATGATTCCCG CACAGCCCTA 
CATGTGGCGG CAGCGGAAGG ACACATTGAC GTTGTCAAGT TTCTGATCGA GGCTTGCAAA 
GTGAATCCTT TTGTCAAGGA CAGGTGGGGC AACATTCCCC TGGATGATGC CGTGCAGTTC 
AATCACCTGG AGGTGGTCAA ACTGCTTCAG GATTACCATG ACTCCTACAT GCTGTCTGAG 
ACTCAAGCTG AGGTAGCAGC TGAGACTCTG TCAAAAGAGA ACTTAGAGAG CATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( XM_006240730.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240792.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant X4, mRNA.

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CloneID	ORa12180
Clone ID Related Accession (Same CDS sequence)	NM_138904.2 , XM_006240730.2
Accession Version	XM_006240730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCTC GACTTGGTGA CCTGCTTTTC TACACCATTG CAGAGGGGCA GGAACGCATC 
CCCATCCATA AGTTCACCAC GGCCCTGAAG GCCACTGGAC TGCAGACGTC AGACCCACGG 
CTCCAGGACT GCATGAGCAA GATGCAGCGC ATGGTCCAAG AGTCCAGCAG TGGTGGCCTC 
TTGGACCGAG AACTCTTCCA AAAGTGTGTG AGCAGCAACA TTGTGCTCCT GACTCAGGCA 
TTCCGGAAGA AGTTTGTCAT TCCTGACTTT GAGGAGTTCA CAGGCCATGT GGATCGTATA 
TTTGAGGATG CCAAAGAGCT CACTGGAGGC AAAGTGGCAG CCTACATCCC TCACCTGGCC 
AAGTCAAACC CAGATCTTTG GGGCGTCTCC CTGTGCACTG TGGATGGGCA GCGACACTCT 
GTGGGCCACA CGAAGATCCC TTTCTGCCTA CAGTCCTGTG TCAAGCCACT CACTTACGCC 
ATCTCCGTGA GCACCTTAGG CACCGACTAC GTGCACAAGT TCGTGGGCAA GGAACCCAGT 
GGTCTGCGCT ATAACAAACT CTCCCTCAAC GAGGAAGGAA TCCCTCATAA CCCCATGGTC 
AATGCTGGTG CTATTGTGGT CAGCTCCCTG ATCAAGATGG ACTGTAACAA AGCAGAGAAG 
TTTGACTTTG TGTTACAGTA TCTGAACAAG ATGGCTGGGA ATGAATTCAT GGGGTTCAGC 
AATGCCACAT TCCAGTCAGA GAAGGAGACT GGGGATCGGA ATTACGCCAT TGGCTATTAC 
CTCAAGGAGA AGAAGTGCTT CCCTAAGGGA GTGGACATGA TGGCTGCCCT TGATCTCTAT 
TTCCAGCTGT GCTCTGTGGA GGTTACCTGT GAGTCAGGCA GTGTCATGGC GGCCACTCTT 
GCCAATGGCG GCATCTGCCC CATCACAGGG GAGAGCGTGC TGAGTGCGGA AGCCGTGCGC 
AACACCCTCA GCCTCATGCA CTCCTGTGGC ATGTATGACT TCTCGGGCCA GTTTGCCTTC 
CACGTGGGCC TGCCAGCCAA GTCAGCTGTG TCGGGAGCCA TCCTCCTGGT TGTACCCAAT 
GTCATGGGGA TGATGTGTCT GTCACCCCCG TTAGACAAGC TGGGGAACAG CCACAGGGGC 
ATCAGCTTCT GCCAGAAGTT GGTGTCTCTG TTTAACTTCC ACAACTACGA CAACCTGCGG 
CACTGTGCTC GGAAGTTAGA CCCACGGAGG GAAGGGGGGG AAGTTCGGAA CAAGACCGTG 
GTGAACCTGT TATTTGCTGC ATATAGTGGA GATGTCTCAG CTCTTCGAAG GTTTGCCTTG 
TCTGCCGTGG ATATGGAGCA GAAGGACTAT GATTCCCGCA CAGCCCTACA TGTGGCGGCA 
GCGGAAGGAC ACATTGACGT TGTCAAGTTT CTGATCGAGG CTTGCAAAGT GAATCCTTTT 
GTCAAGGACA GGTGGGGCAA CATTCCCCTG GATGATGCCG TGCAGTTCAA TCACCTGGAG 
GTGGTCAAAC TGCTTCAGGA TTACCATGAC TCCTACATGC TGTCTGAGAC TCAAGCTGAG 
GTAGCAGCTG AGACTCTGTC AAAAGAGAAC TTAGAGAGCA TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240792.1
CDS	263..1870
Translation	MLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	XM_006240730.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240730.2

ORF Insert Sequence:

ATGTTGCCTC GACTTGGTGA CCTGCTTTTC TACACCATTG CAGAGGGGCA GGAACGCATC 
CCCATCCATA AGTTCACCAC GGCCCTGAAG GCCACTGGAC TGCAGACGTC AGACCCACGG 
CTCCAGGACT GCATGAGCAA GATGCAGCGC ATGGTCCAAG AGTCCAGCAG TGGTGGCCTC 
TTGGACCGAG AACTCTTCCA AAAGTGTGTG AGCAGCAACA TTGTGCTCCT GACTCAGGCA 
TTCCGGAAGA AGTTTGTCAT TCCTGACTTT GAGGAGTTCA CAGGCCATGT GGATCGTATA 
TTTGAGGATG CCAAAGAGCT CACTGGAGGC AAAGTGGCAG CCTACATCCC TCACCTGGCC 
AAGTCAAACC CAGATCTTTG GGGCGTCTCC CTGTGCACTG TGGATGGGCA GCGACACTCT 
GTGGGCCACA CGAAGATCCC TTTCTGCCTA CAGTCCTGTG TCAAGCCACT CACTTACGCC 
ATCTCCGTGA GCACCTTAGG CACCGACTAC GTGCACAAGT TCGTGGGCAA GGAACCCAGT 
GGTCTGCGCT ATAACAAACT CTCCCTCAAC GAGGAAGGAA TCCCTCATAA CCCCATGGTC 
AATGCTGGTG CTATTGTGGT CAGCTCCCTG ATCAAGATGG ACTGTAACAA AGCAGAGAAG 
TTTGACTTTG TGTTACAGTA TCTGAACAAG ATGGCTGGGA ATGAATTCAT GGGGTTCAGC 
AATGCCACAT TCCAGTCAGA GAAGGAGACT GGGGATCGGA ATTACGCCAT TGGCTATTAC 
CTCAAGGAGA AGAAGTGCTT CCCTAAGGGA GTGGACATGA TGGCTGCCCT TGATCTCTAT 
TTCCAGCTGT GCTCTGTGGA GGTTACCTGT GAGTCAGGCA GTGTCATGGC GGCCACTCTT 
GCCAATGGCG GCATCTGCCC CATCACAGGG GAGAGCGTGC TGAGTGCGGA AGCCGTGCGC 
AACACCCTCA GCCTCATGCA CTCCTGTGGC ATGTATGACT TCTCGGGCCA GTTTGCCTTC 
CACGTGGGCC TGCCAGCCAA GTCAGCTGTG TCGGGAGCCA TCCTCCTGGT TGTACCCAAT 
GTCATGGGGA TGATGTGTCT GTCACCCCCG TTAGACAAGC TGGGGAACAG CCACAGGGGC 
ATCAGCTTCT GCCAGAAGTT GGTGTCTCTG TTTAACTTCC ACAACTACGA CAACCTGCGG 
CACTGTGCTC GGAAGTTAGA CCCACGGAGG GAAGGGGGGG AAGTTCGGAA CAAGACCGTG 
GTGAACCTGT TATTTGCTGC ATATAGTGGA GATGTCTCAG CTCTTCGAAG GTTTGCCTTG 
TCTGCCGTGG ATATGGAGCA GAAGGACTAT GATTCCCGCA CAGCCCTACA TGTGGCGGCA 
GCGGAAGGAC ACATTGACGT TGTCAAGTTT CTGATCGAGG CTTGCAAAGT GAATCCTTTT 
GTCAAGGACA GGTGGGGCAA CATTCCCCTG GATGATGCCG TGCAGTTCAA TCACCTGGAG 
GTGGTCAAAC TGCTTCAGGA TTACCATGAC TCCTACATGC TGTCTGAGAC TCAAGCTGAG 
GTAGCAGCTG AGACTCTGTC AAAAGAGAAC TTAGAGAGCA TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( XM_006240728.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240790.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant X1, mRNA.

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CloneID	ORa29729
Clone ID Related Accession (Same CDS sequence)	XM_006240728.3
Accession Version	XM_006240728.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CATGATGCCT CGAACAGTGG CATGTTGCCT CGACTTGGTG ACCTGCTTTT CTACACCATT 
GCAGAGGGGC AGGAACGCAT CCCCATCCAT AAGTTCACCA CGGCCCTGAA GGCCACTGGA 
CTGCAGACGT CAGACCCACG GCTCCAGGAC TGCATGAGCA AGATGCAGCG CATGGTCCAA 
GAGTCCAGCA GTGGTGGCCT CTTGGACCGA GAACTCTTCC AAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACTCAGGC ATTCCGGAAG AAGTTTGTCA TTCCTGACTT TGAGGAGTTC 
ACAGGCCATG TGGATCGTAT ATTTGAGGAT GCCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCACCTGGC CAAGTCAAAC CCAGATCTTT GGGGCGTCTC CCTGTGCACT 
GTGGATGGGC AGCGACACTC TGTGGGCCAC ACGAAGATCC CTTTCTGCCT ACAGTCCTGT 
GTCAAGCCAC TCACTTACGC CATCTCCGTG AGCACCTTAG GCACCGACTA CGTGCACAAG 
TTCGTGGGCA AGGAACCCAG TGGTCTGCGC TATAACAAAC TCTCCCTCAA CGAGGAAGGA 
ATCCCTCATA ACCCCATGGT CAATGCTGGT GCTATTGTGG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGACTTT GTGTTACAGT ATCTGAACAA GATGGCTGGG 
AATGAATTCA TGGGGTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAGAC TGGGGATCGG 
AATTACGCCA TTGGCTATTA CCTCAAGGAG AAGAAGTGCT TCCCTAAGGG AGTGGACATG 
ATGGCTGCCC TTGATCTCTA TTTCCAGCTG TGCTCTGTGG AGGTTACCTG TGAGTCAGGC 
AGTGTCATGG CGGCCACTCT TGCCAATGGC GGCATCTGCC CCATCACAGG GGAGAGCGTG 
CTGAGTGCGG AAGCCGTGCG CAACACCCTC AGCCTCATGC ACTCCTGTGG CATGTATGAC 
TTCTCGGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT GTCGGGAGCC 
ATCCTCCTGG TTGTACCCAA TGTCATGGGG ATGATGTGTC TGTCACCCCC GTTAGACAAG 
CTGGGGAACA GCCACAGGGG CATCAGCTTC TGCCAGAACA AGACCGTGGT GAACCTGTTA 
TTTGCTGCAT ATAGTGGAGA TGTCTCAGCT CTTCGAAGGT TTGCCTTGTC TGCCGTGGAT 
ATGGAGCAGA AGGACTATGA TTCCCGCACA GCCCTACATG TGGCGGCAGC GGAAGGACAC 
ATTGACGTTG TCAAGTTTCT GATCGAGGCT TGCAAAGTGA ATCCTTTTGT CAAGGACAGG 
TGGGGCAACA TTCCCCTGGA TGATGCCGTG CAGTTCAATC ACCTGGAGGT GGTCAAACTG 
CTTCAGGATT ACCATGACTC CTACATGCTG TCTGAGACTC AAGCTGAGGT AGCAGCTGAG 
ACTCTGTCAA AAGAGAACTT AGAGAGCATG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240790.1
CDS	277..1992
Translation	MRSMRALQNALSRAGSHGQRGGWGHPSRGPLLGGGVRYYFGEAAAQGRGTPHSHQPQHSDHDASNSGMLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	XM_006240728.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240728.3

ORF Insert Sequence:

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CATGATGCCT CGAACAGTGG CATGTTGCCT CGACTTGGTG ACCTGCTTTT CTACACCATT 
GCAGAGGGGC AGGAACGCAT CCCCATCCAT AAGTTCACCA CGGCCCTGAA GGCCACTGGA 
CTGCAGACGT CAGACCCACG GCTCCAGGAC TGCATGAGCA AGATGCAGCG CATGGTCCAA 
GAGTCCAGCA GTGGTGGCCT CTTGGACCGA GAACTCTTCC AAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACTCAGGC ATTCCGGAAG AAGTTTGTCA TTCCTGACTT TGAGGAGTTC 
ACAGGCCATG TGGATCGTAT ATTTGAGGAT GCCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCACCTGGC CAAGTCAAAC CCAGATCTTT GGGGCGTCTC CCTGTGCACT 
GTGGATGGGC AGCGACACTC TGTGGGCCAC ACGAAGATCC CTTTCTGCCT ACAGTCCTGT 
GTCAAGCCAC TCACTTACGC CATCTCCGTG AGCACCTTAG GCACCGACTA CGTGCACAAG 
TTCGTGGGCA AGGAACCCAG TGGTCTGCGC TATAACAAAC TCTCCCTCAA CGAGGAAGGA 
ATCCCTCATA ACCCCATGGT CAATGCTGGT GCTATTGTGG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGACTTT GTGTTACAGT ATCTGAACAA GATGGCTGGG 
AATGAATTCA TGGGGTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAGAC TGGGGATCGG 
AATTACGCCA TTGGCTATTA CCTCAAGGAG AAGAAGTGCT TCCCTAAGGG AGTGGACATG 
ATGGCTGCCC TTGATCTCTA TTTCCAGCTG TGCTCTGTGG AGGTTACCTG TGAGTCAGGC 
AGTGTCATGG CGGCCACTCT TGCCAATGGC GGCATCTGCC CCATCACAGG GGAGAGCGTG 
CTGAGTGCGG AAGCCGTGCG CAACACCCTC AGCCTCATGC ACTCCTGTGG CATGTATGAC 
TTCTCGGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT GTCGGGAGCC 
ATCCTCCTGG TTGTACCCAA TGTCATGGGG ATGATGTGTC TGTCACCCCC GTTAGACAAG 
CTGGGGAACA GCCACAGGGG CATCAGCTTC TGCCAGAACA AGACCGTGGT GAACCTGTTA 
TTTGCTGCAT ATAGTGGAGA TGTCTCAGCT CTTCGAAGGT TTGCCTTGTC TGCCGTGGAT 
ATGGAGCAGA AGGACTATGA TTCCCGCACA GCCCTACATG TGGCGGCAGC GGAAGGACAC 
ATTGACGTTG TCAAGTTTCT GATCGAGGCT TGCAAAGTGA ATCCTTTTGT CAAGGACAGG 
TGGGGCAACA TTCCCCTGGA TGATGCCGTG CAGTTCAATC ACCTGGAGGT GGTCAAACTG 
CTTCAGGATT ACCATGACTC CTACATGCTG TCTGAGACTC AAGCTGAGGT AGCAGCTGAG 
ACTCTGTCAA AAGAGAACTT AGAGAGCATG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( XM_017594653.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450142.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa61893
Clone ID Related Accession (Same CDS sequence)	XM_017594653.1
Accession Version	XM_017594653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAGA TGCAGCGCAT GGTCCAAGAG TCCAGCAGTG GTGGCCTCTT GGACCGAGAA 
CTCTTCCAAA AGTGTGTGAG CAGCAACATT GTGCTCCTGA CTCAGGCATT CCGGAAGAAG 
TTTGTCATTC CTGACTTTGA GGAGTTCACA GGCCATGTGG ATCGTATATT TGAGGATGCC 
AAAGAGCTCA CTGGAGGCAA AGTGGCAGCC TACATCCCTC ACCTGGCCAA GTCAAACCCA 
GATCTTTGGG GCGTCTCCCT GTGCACTGTG GATGGGCAGC GACACTCTGT GGGCCACACG 
AAGATCCCTT TCTGCCTACA GTCCTGTGTC AAGCCACTCA CTTACGCCAT CTCCGTGAGC 
ACCTTAGGCA CCGACTACGT GCACAAGTTC GTGGGCAAGG AACCCAGTGG TCTGCGCTAT 
AACAAACTCT CCCTCAACGA GGAAGGAATC CCTCATAACC CCATGGTCAA TGCTGGTGCT 
ATTGTGGTCA GCTCCCTGAT CAAGATGGAC TGTAACAAAG CAGAGAAGTT TGACTTTGTG 
TTACAGTATC TGAACAAGAT GGCTGGGAAT GAATTCATGG GGTTCAGCAA TGCCACATTC 
CAGTCAGAGA AGGAGACTGG GGATCGGAAT TACGCCATTG GCTATTACCT CAAGGAGAAG 
AAGTGCTTCC CTAAGGGAGT GGACATGATG GCTGCCCTTG ATCTCTATTT CCAGCTGTGC 
TCTGTGGAGG TTACCTGTGA GTCAGGCAGT GTCATGGCGG CCACTCTTGC CAATGGCGGC 
ATCTGCCCCA TCACAGGGGA GAGCGTGCTG AGTGCGGAAG CCGTGCGCAA CACCCTCAGC 
CTCATGCACT CCTGTGGCAT GTATGACTTC TCGGGCCAGT TTGCCTTCCA CGTGGGCCTG 
CCAGCCAAGT CAGCTGTGTC GGGAGCCATC CTCCTGGTTG TACCCAATGT CATGGGGATG 
ATGTGTCTGT CACCCCCGTT AGACAAGCTG GGGAACAGCC ACAGGGGCAT CAGCTTCTGC 
CAGAAGTTGG TGTCTCTGTT TAACTTCCAC AACTACGACA ACCTGCGGCA CTGTGCTCGG 
AAGTTAGACC CACGGAGGGA AGGGGGGGAA GTTCGGAACA AGACCGTGGT GAACCTGTTA 
TTTGCTGCAT ATAGTGGAGA TGTCTCAGCT CTTCGAAGGT TTGCCTTGTC TGCCGTGGAT 
ATGGAGCAGA AGGACTATGA TTCCCGCACA GCCCTACATG TGGCGGCAGC GGAAGGACAC 
ATTGACGTTG TCAAGTTTCT GATCGAGGCT TGCAAAGTGA ATCCTTTTGT CAAGGACAGG 
TGGGGCAACA TTCCCCTGGA TGATGCCGTG CAGTTCAATC ACCTGGAGGT GGTCAAACTG 
CTTCAGGATT ACCATGACTC CTACATGCTG TCTGAGACTC AAGCTGAGGT AGCAGCTGAG 
ACTCTGTCAA AAGAGAACTT AGAGAGCATG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450142.1
CDS	136..1611
Translation	MSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	XM_017594653.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594653.1

ORF Insert Sequence:

ATGAGCAAGA TGCAGCGCAT GGTCCAAGAG TCCAGCAGTG GTGGCCTCTT GGACCGAGAA 
CTCTTCCAAA AGTGTGTGAG CAGCAACATT GTGCTCCTGA CTCAGGCATT CCGGAAGAAG 
TTTGTCATTC CTGACTTTGA GGAGTTCACA GGCCATGTGG ATCGTATATT TGAGGATGCC 
AAAGAGCTCA CTGGAGGCAA AGTGGCAGCC TACATCCCTC ACCTGGCCAA GTCAAACCCA 
GATCTTTGGG GCGTCTCCCT GTGCACTGTG GATGGGCAGC GACACTCTGT GGGCCACACG 
AAGATCCCTT TCTGCCTACA GTCCTGTGTC AAGCCACTCA CTTACGCCAT CTCCGTGAGC 
ACCTTAGGCA CCGACTACGT GCACAAGTTC GTGGGCAAGG AACCCAGTGG TCTGCGCTAT 
AACAAACTCT CCCTCAACGA GGAAGGAATC CCTCATAACC CCATGGTCAA TGCTGGTGCT 
ATTGTGGTCA GCTCCCTGAT CAAGATGGAC TGTAACAAAG CAGAGAAGTT TGACTTTGTG 
TTACAGTATC TGAACAAGAT GGCTGGGAAT GAATTCATGG GGTTCAGCAA TGCCACATTC 
CAGTCAGAGA AGGAGACTGG GGATCGGAAT TACGCCATTG GCTATTACCT CAAGGAGAAG 
AAGTGCTTCC CTAAGGGAGT GGACATGATG GCTGCCCTTG ATCTCTATTT CCAGCTGTGC 
TCTGTGGAGG TTACCTGTGA GTCAGGCAGT GTCATGGCGG CCACTCTTGC CAATGGCGGC 
ATCTGCCCCA TCACAGGGGA GAGCGTGCTG AGTGCGGAAG CCGTGCGCAA CACCCTCAGC 
CTCATGCACT CCTGTGGCAT GTATGACTTC TCGGGCCAGT TTGCCTTCCA CGTGGGCCTG 
CCAGCCAAGT CAGCTGTGTC GGGAGCCATC CTCCTGGTTG TACCCAATGT CATGGGGATG 
ATGTGTCTGT CACCCCCGTT AGACAAGCTG GGGAACAGCC ACAGGGGCAT CAGCTTCTGC 
CAGAAGTTGG TGTCTCTGTT TAACTTCCAC AACTACGACA ACCTGCGGCA CTGTGCTCGG 
AAGTTAGACC CACGGAGGGA AGGGGGGGAA GTTCGGAACA AGACCGTGGT GAACCTGTTA 
TTTGCTGCAT ATAGTGGAGA TGTCTCAGCT CTTCGAAGGT TTGCCTTGTC TGCCGTGGAT 
ATGGAGCAGA AGGACTATGA TTCCCGCACA GCCCTACATG TGGCGGCAGC GGAAGGACAC 
ATTGACGTTG TCAAGTTTCT GATCGAGGCT TGCAAAGTGA ATCCTTTTGT CAAGGACAGG 
TGGGGCAACA TTCCCCTGGA TGATGCCGTG CAGTTCAATC ACCTGGAGGT GGTCAAACTG 
CTTCAGGATT ACCATGACTC CTACATGCTG TCTGAGACTC AAGCTGAGGT AGCAGCTGAG 
ACTCTGTCAA AAGAGAACTT AGAGAGCATG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( XM_017594654.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450143.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa61894
Clone ID Related Accession (Same CDS sequence)	XM_017594654.1
Accession Version	XM_017594654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCG TATATTTGAG GATGCCAAAG AGCTCACTGG AGGCAAAACA CTCTGTGGGC 
CACACGAAGA TCCCTTTCTG CCTACAGTCC TGTGTCAAGC CACTCACTTA CGCCATCTCC 
GTGAGCACCT TAGGCACCGA CTACGTGCAC AAGTTCGTGG GCAAGGAACC CAGTGGTCTG 
CGCTATAACA AACTCTCCCT CAACGAGGAA GGAATCCCTC ATAACCCCAT GGTCAATGCT 
GGTGCTATTG TGGTCAGCTC CCTGATCAAG ATGGACTGTA ACAAAGCAGA GAAGTTTGAC 
TTTGTGTTAC AGTATCTGAA CAAGATGGCT GGGAATGAAT TCATGGGGTT CAGCAATGCC 
ACATTCCAGT CAGAGAAGGA GACTGGGGAT CGGAATTACG CCATTGGCTA TTACCTCAAG 
GAGAAGAAGT GCTTCCCTAA GGGAGTGGAC ATGATGGCTG CCCTTGATCT CTATTTCCAG 
CTGTGCTCTG TGGAGGTTAC CTGTGAGTCA GGCAGTGTCA TGGCGGCCAC TCTTGCCAAT 
GGCGGCATCT GCCCCATCAC AGGGGAGAGC GTGCTGAGTG CGGAAGCCGT GCGCAACACC 
CTCAGCCTCA TGCACTCCTG TGGCATGTAT GACTTCTCGG GCCAGTTTGC CTTCCACGTG 
GGCCTGCCAG CCAAGTCAGC TGTGTCGGGA GCCATCCTCC TGGTTGTACC CAATGTCATG 
GGGATGATGT GTCTGTCACC CCCGTTAGAC AAGCTGGGGA ACAGCCACAG GGGCATCAGC 
TTCTGCCAGA AGTTGGTGTC TCTGTTTAAC TTCCACAACT ACGACAACCT GCGGCACTGT 
GCTCGGAAGT TAGACCCACG GAGGGAAGGG GGGGAAGTTC GGAACAAGAC CGTGGTGAAC 
CTGTTATTTG CTGCATATAG TGGAGATGTC TCAGCTCTTC GAAGGTTTGC CTTGTCTGCC 
GTGGATATGG AGCAGAAGGA CTATGATTCC CGCACAGCCC TACATGTGGC GGCAGCGGAA 
GGACACATTG ACGTTGTCAA GTTTCTGATC GAGGCTTGCA AAGTGAATCC TTTTGTCAAG 
GACAGGTGGG GCAACATTCC CCTGGATGAT GCCGTGCAGT TCAATCACCT GGAGGTGGTC 
AAACTGCTTC AGGATTACCA TGACTCCTAC ATGCTGTCTG AGACTCAAGC TGAGGTAGCA 
GCTGAGACTC TGTCAAAAGA GAACTTAGAG AGCATGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450143.1
CDS	82..1323
Translation	MWIVYLRMPKSSLEAKHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	XM_017594654.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594654.1

ORF Insert Sequence:

ATGTGGATCG TATATTTGAG GATGCCAAAG AGCTCACTGG AGGCAAAACA CTCTGTGGGC 
CACACGAAGA TCCCTTTCTG CCTACAGTCC TGTGTCAAGC CACTCACTTA CGCCATCTCC 
GTGAGCACCT TAGGCACCGA CTACGTGCAC AAGTTCGTGG GCAAGGAACC CAGTGGTCTG 
CGCTATAACA AACTCTCCCT CAACGAGGAA GGAATCCCTC ATAACCCCAT GGTCAATGCT 
GGTGCTATTG TGGTCAGCTC CCTGATCAAG ATGGACTGTA ACAAAGCAGA GAAGTTTGAC 
TTTGTGTTAC AGTATCTGAA CAAGATGGCT GGGAATGAAT TCATGGGGTT CAGCAATGCC 
ACATTCCAGT CAGAGAAGGA GACTGGGGAT CGGAATTACG CCATTGGCTA TTACCTCAAG 
GAGAAGAAGT GCTTCCCTAA GGGAGTGGAC ATGATGGCTG CCCTTGATCT CTATTTCCAG 
CTGTGCTCTG TGGAGGTTAC CTGTGAGTCA GGCAGTGTCA TGGCGGCCAC TCTTGCCAAT 
GGCGGCATCT GCCCCATCAC AGGGGAGAGC GTGCTGAGTG CGGAAGCCGT GCGCAACACC 
CTCAGCCTCA TGCACTCCTG TGGCATGTAT GACTTCTCGG GCCAGTTTGC CTTCCACGTG 
GGCCTGCCAG CCAAGTCAGC TGTGTCGGGA GCCATCCTCC TGGTTGTACC CAATGTCATG 
GGGATGATGT GTCTGTCACC CCCGTTAGAC AAGCTGGGGA ACAGCCACAG GGGCATCAGC 
TTCTGCCAGA AGTTGGTGTC TCTGTTTAAC TTCCACAACT ACGACAACCT GCGGCACTGT 
GCTCGGAAGT TAGACCCACG GAGGGAAGGG GGGGAAGTTC GGAACAAGAC CGTGGTGAAC 
CTGTTATTTG CTGCATATAG TGGAGATGTC TCAGCTCTTC GAAGGTTTGC CTTGTCTGCC 
GTGGATATGG AGCAGAAGGA CTATGATTCC CGCACAGCCC TACATGTGGC GGCAGCGGAA 
GGACACATTG ACGTTGTCAA GTTTCTGATC GAGGCTTGCA AAGTGAATCC TTTTGTCAAG 
GACAGGTGGG GCAACATTCC CCTGGATGAT GCCGTGCAGT TCAATCACCT GGAGGTGGTC 
AAACTGCTTC AGGATTACCA TGACTCCTAC ATGCTGTCTG AGACTCAAGC TGAGGTAGCA 
GCTGAGACTC TGTCAAAAGA GAACTTAGAG AGCATGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gls2 ( XM_017594652.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450141.1 Rattus norvegicus glutaminase 2 (Gls2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61892
Clone ID Related Accession (Same CDS sequence)	XM_017594652.1
Accession Version	XM_017594652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutaminase liver isoform, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CAGTGTGTGA GCAGCAACAT TGTGCTCCTG ACTCAGGCAT TCCGGAAGAA GTTTGTCATT 
CCTGACTTTG AGGAGTTCAC AGGCCATGTG GATCGTATAT TTGAGGATGC CAAAGAGCTC 
ACTGGAGGCA AAGTGGCAGC CTACATCCCT CACCTGGCCA AGTCAAACCC AGATCTTTGG 
GGCGTCTCCC TGTGCACTGT GGATGGGCAG CGACACTCTG TGGGCCACAC GAAGATCCCT 
TTCTGCCTAC AGTCCTGTGT CAAGCCACTC ACTTACGCCA TCTCCGTGAG CACCTTAGGC 
ACCGACTACG TGCACAAGTT CGTGGGCAAG GAACCCAGTG GTCTGCGCTA TAACAAACTC 
TCCCTCAACG AGGAAGGAAT CCCTCATAAC CCCATGGTCA ATGCTGGTGC TATTGTGGTC 
AGCTCCCTGA TCAAGATGGA CTGTAACAAA GCAGAGAAGT TTGACTTTGT GTTACAGTAT 
CTGAACAAGA TGGCTGGGAA TGAATTCATG GGGTTCAGCA ATGCCACATT CCAGTCAGAG 
AAGGAGACTG GGGATCGGAA TTACGCCATT GGCTATTACC TCAAGGAGAA GAAGTGCTTC 
CCTAAGGGAG TGGACATGAT GGCTGCCCTT GATCTCTATT TCCAGCTGTG CTCTGTGGAG 
GTTACCTGTG AGTCAGGCAG TGTCATGGCG GCCACTCTTG CCAATGGCGG CATCTGCCCC 
ATCACAGGGG AGAGCGTGCT GAGTGCGGAA GCCGTGCGCA ACACCCTCAG CCTCATGCAC 
TCCTGTGGCA TGTATGACTT CTCGGGCCAG TTTGCCTTCC ACGTGGGCCT GCCAGCCAAG 
TCAGCTGTGT CGGGAGCCAT CCTCCTGGTT GTACCCAATG TCATGGGGAT GATGTGTCTG 
TCACCCCCGT TAGACAAGCT GGGGAACAGC CACAGGGGCA TCAGCTTCTG CCAGAAGTTG 
GTGTCTCTGT TTAACTTCCA CAACTACGAC AACCTGCGGC ACTGTGCTCG GAAGTTAGAC 
CCACGGAGGG AAGGGGGGGA AGTTCGGAAC AAGACCGTGG TGAACCTGTT ATTTGCTGCA 
TATAGTGGAG ATGTCTCAGC TCTTCGAAGG TTTGCCTTGT CTGCCGTGGA TATGGAGCAG 
AAGGACTATG ATTCCCGCAC AGCCCTACAT GTGGCGGCAG CGGAAGGACA CATTGACGTT 
GTCAAGTTTC TGATCGAGGC TTGCAAAGTG AATCCTTTTG TCAAGGACAG GTGGGGCAAC 
ATTCCCCTGG ATGATGCCGT GCAGTTCAAT CACCTGGAGG TGGTCAAACT GCTTCAGGAT 
TACCATGACT CCTACATGCT GTCTGAGACT CAAGCTGAGG TAGCAGCTGA GACTCTGTCA 
AAAGAGAACT TAGAGAGCAT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450141.1
CDS	277..1863
Translation	MRSMRALQNALSRAGSHGQRGGWGHPSRGPLLGGGVRYYFGEAAAQGRGTPHSHQPQHSDQCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV

Target ORF information:

RefSeq Version	XM_017594652.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutaminase 2 (Gls2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594652.1

ORF Insert Sequence:

ATGCGCTCCA TGAGGGCTCT GCAGAACGCG CTGAGCCGCG CGGGCAGCCA CGGCCAGAGG 
GGAGGCTGGG GTCACCCGAG CCGGGGCCCG CTCCTGGGCG GGGGTGTCCG GTACTACTTC 
GGTGAGGCCG CGGCGCAGGG CAGGGGGACG CCGCACAGCC ACCAGCCGCA GCACTCGGAC 
CAGTGTGTGA GCAGCAACAT TGTGCTCCTG ACTCAGGCAT TCCGGAAGAA GTTTGTCATT 
CCTGACTTTG AGGAGTTCAC AGGCCATGTG GATCGTATAT TTGAGGATGC CAAAGAGCTC 
ACTGGAGGCA AAGTGGCAGC CTACATCCCT CACCTGGCCA AGTCAAACCC AGATCTTTGG 
GGCGTCTCCC TGTGCACTGT GGATGGGCAG CGACACTCTG TGGGCCACAC GAAGATCCCT 
TTCTGCCTAC AGTCCTGTGT CAAGCCACTC ACTTACGCCA TCTCCGTGAG CACCTTAGGC 
ACCGACTACG TGCACAAGTT CGTGGGCAAG GAACCCAGTG GTCTGCGCTA TAACAAACTC 
TCCCTCAACG AGGAAGGAAT CCCTCATAAC CCCATGGTCA ATGCTGGTGC TATTGTGGTC 
AGCTCCCTGA TCAAGATGGA CTGTAACAAA GCAGAGAAGT TTGACTTTGT GTTACAGTAT 
CTGAACAAGA TGGCTGGGAA TGAATTCATG GGGTTCAGCA ATGCCACATT CCAGTCAGAG 
AAGGAGACTG GGGATCGGAA TTACGCCATT GGCTATTACC TCAAGGAGAA GAAGTGCTTC 
CCTAAGGGAG TGGACATGAT GGCTGCCCTT GATCTCTATT TCCAGCTGTG CTCTGTGGAG 
GTTACCTGTG AGTCAGGCAG TGTCATGGCG GCCACTCTTG CCAATGGCGG CATCTGCCCC 
ATCACAGGGG AGAGCGTGCT GAGTGCGGAA GCCGTGCGCA ACACCCTCAG CCTCATGCAC 
TCCTGTGGCA TGTATGACTT CTCGGGCCAG TTTGCCTTCC ACGTGGGCCT GCCAGCCAAG 
TCAGCTGTGT CGGGAGCCAT CCTCCTGGTT GTACCCAATG TCATGGGGAT GATGTGTCTG 
TCACCCCCGT TAGACAAGCT GGGGAACAGC CACAGGGGCA TCAGCTTCTG CCAGAAGTTG 
GTGTCTCTGT TTAACTTCCA CAACTACGAC AACCTGCGGC ACTGTGCTCG GAAGTTAGAC 
CCACGGAGGG AAGGGGGGGA AGTTCGGAAC AAGACCGTGG TGAACCTGTT ATTTGCTGCA 
TATAGTGGAG ATGTCTCAGC TCTTCGAAGG TTTGCCTTGT CTGCCGTGGA TATGGAGCAG 
AAGGACTATG ATTCCCGCAC AGCCCTACAT GTGGCGGCAG CGGAAGGACA CATTGACGTT 
GTCAAGTTTC TGATCGAGGC TTGCAAAGTG AATCCTTTTG TCAAGGACAG GTGGGGCAAC 
ATTCCCCTGG ATGATGCCGT GCAGTTCAAT CACCTGGAGG TGGTCAAACT GCTTCAGGAT 
TACCATGACT CCTACATGCT GTCTGAGACT CAAGCTGAGG TAGCAGCTGA GACTCTGTCA 
AAAGAGAACT TAGAGAGCAT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Gls and Gls2 glutaminase isoforms in astrocytes.
Glia63(3)365-82(2015 Mar)
Cardona C,S?nchez-Mej?as E,D?vila JC,Mart?n-Rufi?n M,Campos-Sandoval JA,Vitorica J,Alonso FJ,Mat?s JM,Segura JA,Norenberg MD,Rama Rao KV,Jayakumar AR,Guti?rrez A,M?rquez J

Phosphate-dependent glutaminase response to liver injury and hyperbaric oxygenation.
Bulletin of experimental biology and medicine157(3)299-301(2014 Jul)
Savilov PN,Yakovlev VN

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.
PloS one7(6)e38380(2012)
Mart?n-Rufi?n M,Tosina M,Campos-Sandoval JA,Manzanares E,Lobo C,Segura JA,Alonso FJ,Mat?s JM,M?rquez J

Phosphate-activated glutaminase (GLS2), a p53-inducible regulator of glutamine metabolism and reactive oxygen species.
Proceedings of the National Academy of Sciences of the United States of America107(16)7461-6(2010 Apr)
Suzuki S,Tanaka T,Poyurovsky MV,Nagano H,Mayama T,Ohkubo S,Lokshin M,Hosokawa H,Nakayama T,Suzuki Y,Sugano S,Sato E,Nagao T,Yokote K,Tatsuno I,Prives C

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Gls2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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