Obp1f cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Obp1f
Entrez Gene ID	192267
Full Name	odorant binding protein I f
Synonyms	Obp-1f
General protein information	Preferred Names odorant binding protein I f Names odorant-binding protein OBP odorant-binding protein 1F
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq31
Summary	selectively binds odorants and may be involved in carrying odorants within the nasal epithelium to putative olfactory receptors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138903.1	NP_620258.1	odorant-binding protein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	OBP	XP_002700515.3
Canis lupus familiaris (dog)	LOC100684897	XP_003435497.1
Canis lupus familiaris (dog)	LOC100688842	XP_003435564.2
Rattus norvegicus (Norway rat)	Obp1f	NP_620258.1
Canis lupus familiaris (dog)	OBP	NP_001177855.1
Bos taurus (cattle)	LOC505052	XP_002700514.1
Mus musculus (house mouse)	Gm14744	NP_001079013.1
Bos taurus (cattle)	LOC101904044	XP_005228600.1
Mus musculus (house mouse)	5430402E10Rik	NP_082044.1
Rattus norvegicus (Norway rat)	LOC100362409	XP_006257256.1

Gene Ontology
Bibliography

Related articles in PubMed

Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals.
Borysik AJ, Briand L, Taylor AJ, Scott DJ
Journal of molecular biology404(3)372-80(2010 Dec)

Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?
Badonnel K, Durieux D, Monnerie R, Gr?bert D, Salesse R, Caillol M, Baly C
Cell and tissue research338(1)53-66(2009 Oct)

Structure of rat odorant-binding protein OBP1 at 1.6 A resolution.
White SA, Briand L, Scott DJ, Borysik AJ
Acta crystallographica. Section D, Biological crystallography65(Pt 5)403-10(2009 May)

Transcription profile analysis reveals that OBP-1F mRNA is downregulated in the olfactory mucosa following food deprivation.
Badonnel K, Denis JB, Caillol M, Monnerie R, Piumi F, Potier MC, Salesse R, Baly C
Chemical senses32(7)697-710(2007 Sep)

Odorant binding and conformational dynamics in the odorant-binding protein.
Hajjar E, Perahia D, D?bat H, Nespoulous C, Robert CH
The Journal of biological chemistry281(40)29929-37(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

measured the effect of odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds
Title: Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals.

Results identify a novel population of odorant-binding protein-1f-secreting cells displaying morphological and functional characteristics similar to those of epithelial mucous cells.
Title: Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?

Comparisons are made between this 1.6A resolution structure and a modelled OBP1 structure based on aphrodisin, and with OPB2 and OBP3. It is monomeric.
Title: Structure of rat odorant-binding protein OBP1 at 1.6 A resolution.

The fine transcriptional control of OBP-1F in the olfactory mucosa following food deprivation could be efficient only at the local level, close to its site of secretion to participate in the perireceptor events of the olfactory signal reception.
Title: Transcription profile analysis reveals that OBP-1F mRNA is downregulated in the olfactory mucosa following food deprivation.

Lipocalin strand pair opening in the presence of ligand is observed, consistent with potential role in olfactive receptor recognition.
Title: Odorant binding and conformational dynamics in the odorant-binding protein.

The following Obp1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Obp1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42672	NM_138903.1 Latest version!	Rattus norvegicus odorant binding protein I f (Obp1f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Obp1f ( NM_138903.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620258.1 Rattus norvegicus odorant binding protein I f (Obp1f), mRNA.

Price & Availability↑

CloneID	ORa42672
Clone ID Related Accession (Same CDS sequence)	NM_138903.1
Accession Version	NM_138903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	odorant-binding protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03093.1. ##Evidence-Data-START## Transcript exon combination :: J03093.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGAAGT TTCTGCTGAT TGTTCTTGCA TTAGGTGTAT CCTGTGCACA TCATGAAAAT 
CTTGATATCA GTCCCTCAGA GGTTAATGGG GACTGGCGCA CCCTTTACAT AGTTGCAGAT 
AATGTGGAGA AGGTAGCAGA AGGTGGATCC CTGAGAGCTT ACTTTCAGCA CATGGAATGT 
GGTGATGAAT GCCAGGAACT CAAAATCATA TTCAATGTCA AGTTGGACAG TGAATGTCAG 
ACACACACTG TTGTGGGACA AAAACATGAA GATGGGCGGT ACACTACTGA CTACTCTGGT 
AGAAATTACT TCCATGTTTT GAAGAAGACA GATGACATTA TTTTCTTTCA CAACGTTAAT 
GTCGATGAGA GTGGAAGGAG ACAATGTGAT TTAGTTGCTG GGAAAAGAGA GGACCTGAAC 
AAAGCACAGA AGCAGGAGCT TAGGAAGCTG GCTGAGGAGT ATAATATTCC AAATGAGAAT 
ACCCAGCACT TGGTGCCCAC AGACACTTGT AACCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620258.1
CDS	17..535
Translation	MVKFLLIVLALGVSCAHHENLDISPSEVNGDWRTLYIVADNVEKVAEGGSLRAYFQHMECGDECQELKIIFNVKLDSECQTHTVVGQKHEDGRYTTDYSGRNYFHVLKKTDDIIFFHNVNVDESGRRQCDLVAGKREDLNKAQKQELRKLAEEYNIPNENTQHLVPTDTCNQ

Target ORF information:

RefSeq Version	NM_138903.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus odorant binding protein I f (Obp1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138903.1

ORF Insert Sequence:

ATGGTGAAGT TTCTGCTGAT TGTTCTTGCA TTAGGTGTAT CCTGTGCACA TCATGAAAAT 
CTTGATATCA GTCCCTCAGA GGTTAATGGG GACTGGCGCA CCCTTTACAT AGTTGCAGAT 
AATGTGGAGA AGGTAGCAGA AGGTGGATCC CTGAGAGCTT ACTTTCAGCA CATGGAATGT 
GGTGATGAAT GCCAGGAACT CAAAATCATA TTCAATGTCA AGTTGGACAG TGAATGTCAG 
ACACACACTG TTGTGGGACA AAAACATGAA GATGGGCGGT ACACTACTGA CTACTCTGGT 
AGAAATTACT TCCATGTTTT GAAGAAGACA GATGACATTA TTTTCTTTCA CAACGTTAAT 
GTCGATGAGA GTGGAAGGAG ACAATGTGAT TTAGTTGCTG GGAAAAGAGA GGACCTGAAC 
AAAGCACAGA AGCAGGAGCT TAGGAAGCTG GCTGAGGAGT ATAATATTCC AAATGAGAAT 
ACCCAGCACT TGGTGCCCAC AGACACTTGT AACCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals.
Journal of molecular biology404(3)372-80(2010 Dec)
Borysik AJ,Briand L,Taylor AJ,Scott DJ

Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?
Cell and tissue research338(1)53-66(2009 Oct)
Badonnel K,Durieux D,Monnerie R,Gr?bert D,Salesse R,Caillol M,Baly C

Structure of rat odorant-binding protein OBP1 at 1.6 A resolution.
Acta crystallographica. Section D, Biological crystallography65(Pt 5)403-10(2009 May)
White SA,Briand L,Scott DJ,Borysik AJ

Transcription profile analysis reveals that OBP-1F mRNA is downregulated in the olfactory mucosa following food deprivation.
Chemical senses32(7)697-710(2007 Sep)
Badonnel K,Denis JB,Caillol M,Monnerie R,Piumi F,Potier MC,Salesse R,Baly C

Odorant binding and conformational dynamics in the odorant-binding protein.
The Journal of biological chemistry281(40)29929-37(2006 Oct)
Hajjar E,Perahia D,D?bat H,Nespoulous C,Robert CH