Hip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hip1
Entrez Gene ID	192154
Full Name	huntingtin interacting protein 1
General protein information	Preferred Names huntingtin interacting protein 1 Names huntingtin-interacting protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	human homolog binds clathrin and the clathrin adaptor AP2; may play a role in clathrin-mediated endocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249100.3	XP_006249162.1	huntingtin-interacting protein 1 isoform X4
NM_001100475.1	NP_001093945.1	huntingtin-interacting protein 1
XM_017598253.1	XP_017453742.1	huntingtin-interacting protein 1 isoform X1
XM_006249099.3	XP_006249161.1	huntingtin-interacting protein 1 isoform X3
XM_017598252.1	XP_017453741.1	huntingtin-interacting protein 1 isoform X1
XM_006249098.3	XP_006249160.1	huntingtin-interacting protein 1 isoform X2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02949	XP_003720758.1
Canis lupus familiaris (dog)	HIP1	XP_546932.2
Danio rerio (zebrafish)	hip1	NP_001159677.1
Caenorhabditis elegans (roundworm)	hipr-1	NP_498925.1
Pan troglodytes (chimpanzee)	HIP1	XP_003318566.1
	HIP1	XP_001109894.1
Mus musculus (house mouse)	Hip1	NP_666113.2
Rattus norvegicus (Norway rat)	Hip1	NP_001093945.1
Xenopus tropicalis (tropical clawed frog)	hip1	XP_002933593.2
Homo sapiens (human)	HIP1	NP_005329.3
Bos taurus (cattle)	HIP1	NP_001179007.1
Schizosaccharomyces pombe (fission yeast)	end4	NP_594069.2
Gallus gallus (chicken)	HIP1	XP_001232953.2
Neurospora crassa	NCU11202	XP_964240.1

Gene Ontology
Bibliography

Related articles in PubMed

The Sla2p/HIP1/HIP1R family: similar structure, similar function in endocytosis?
Gottfried I, Ehrlich M, Ashery U
Biochemical Society transactions38(Pt 1)187-91(2010 Feb)

Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain.
Wilbur JD, Chen CY, Manalo V, Hwang PK, Fletterick RJ, Brodsky FM
The Journal of biological chemistry283(47)32870-9(2008 Nov)

Partially overlapping distribution of epsin1 and HIP1 at the synapse: analysis by immunoelectron microscopy.
Yao PJ, Bushlin I, Petralia RS
The Journal of comparative neurology494(2)368-79(2006 Jan)

Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo.
Chen CY, Brodsky FM
The Journal of biological chemistry280(7)6109-17(2005 Feb)

HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains.
Hyun TS, Rao DS, Saint-Dic D, Michael LE, Kumar PD, Bradley SV, Mizukami IF, Oravecz-Wilson KI, Ross TS
The Journal of biological chemistry279(14)14294-306(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

epsin1 is located across both sides of the synapse, whereas HIP1 displays a preference for the postsynaptic compartment; results suggest a dual role for epsin1 and huntingtin-interacting protein 1 in the synapse
Title: Partially overlapping distribution of epsin1 and HIP1 at the synapse: analysis by immunoelectron microscopy.

HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain.
Title: HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain.

The following Hip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa57776	XM_017598253.1 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa57776	XM_017598252.1 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa11317	NM_001100475.1 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$755.30 $1079.00
ORa34730	XM_006249098.3 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa34731	XM_006249099.3 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa34732	XM_006249100.3 Latest version!	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hip1 ( XM_017598253.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453742.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57776
Clone ID Related Accession (Same CDS sequence)	XM_017598253.1 , XM_017598252.1
Accession Version	XM_017598253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCA GCAAAGCCTG CTCCCTTGGG ACAGCTTGGG ACAGCTGGTC TGGGAGTGCT 
GCCCACCTCT GCGCTACCCC CTCTCCCAAG CGAGGTACTC GAGAGGACCC AGAGGGCCCT 
TGGATGGTCT CTTGGCCCTC GTCAATACCA AGGACGGTCA GCGTCAATAA GGCCATTAAT 
ACGCAGGAAG TGGCCGTGAA GGAAAAACAT GCCAGGACGT GTATTCTGGG CACCCACCAT 
GAGAAAGGGG CGCAGACCTT CTGGTCTGTG GTCAACCGAC TGCCTCTCTC CAGCAACGCC 
ATGCTGTGCT GGAAGTTCTG CCACGTGTTC CACAAACTCC TTCGAGACGG ACATCCGAAC 
GTGCTGAAAG ACTCTCTCAG ATACAAAAAT GAGTTAAGCG ACATGAGCAG GATGTGGGGC 
CACCTGAGTG AGGGGTATGG ACAGCTCTGC AGCATCTACC TCAAACTGCT GAGAACAAGG 
ATGGAGTACC ACACCAAAAA TCCCAGGTTC CCAGGTAACC TCCAGATGAG CGACCGGCAG 
CTGGATGAAG CTGGGGAAAG TGATGTTAAC AACTTTTTCC AGCTCACAGT GGAGATGTTT 
GATTACCTGG AGTGCGAACT GAACCTCTTT CAAACTGTGT TCAACTCCTT GGACATGTCC 
CGCTCAGTGT CCGTGACCAC AGCTGGCCAG TGCCGCCTCG CCCCGCTCAT CCAGGTCATC 
CTGGACTGCA GTCACCTCTA TGACTACACT GTCAAGCTGC TCTTCAAGCT CCACTCCTGT 
CTTCCAGCTG ACACCCTGCA GGGCCACCGA GACCGCTTCA TGGAGCAGTT CACAAAGTTG 
AAAGATCTGT TTCAGCGCTC CAGCAACCTT CAGTACTTCA AGCGGCTCAT TCAGATCCCC 
CAGTTGCCTG AGAATCCACC CAACTTCCTA CGAGCCTCGG CCCTGTCCGA GCACATCAGT 
CCTGTGGTGG TGATCCCGGC TGAGGTGTCG TCTCCAGACA GTGAGCCCGT CCTGGAGAAG 
GATGACCTCA TGGACATGGA TGCCTCCCAG CAGAGTTTGT TTGACAACAA GTTTGATGAC 
ATCTTCGGCA GCTCGTTGAG CAGTGACCCC TTCAATTTCA ACAATCAAAA TGGCGTGAAC 
AAGGATGAGA AGGACCACTT GATTGAACGC CTCTACAGAG AGATCAGTGG ACTGACAGGA 
CAGCTGGACA ACGTGAAGAT TGAGAGCCAG CGGGCCATGC TGCAGCTGAA GGGTCGCGTG 
AGTGAGCTGG AAGCAGAGCT GGCCGAGCAG CAGCACCTGG GACGGCAGGC TACGGATGAC 
TGCGAGTTCC TGCGCACGGA GCTGGATGAA CTGAAGAGGC AGCGAGAGGA CACTGAGAAG 
GCGCAGCGCA GCCTGACTGA GATAGAAAAA AAGGCCCAGG CCAATGAACA GCGGTATAGC 
AAGTTAAAAG AGAAGTACAG TGAACTGGTG CAGAACCATG CTGATCTGCT GCGGAAGAAT 
GCAGAGGTGA CCAAGCAGGT GTCTGTGGCC CGGCAAGCCC AGGTGGATTT GGAAAGAGAG 
AAAAAAGAAC TAGCGGATTC CTTTGCACGT GTAAGCGAGC AGACCCAGCG GAAGACTCAA 
GAGCAACAGG ATGTTCTAGA GACCCTGAAG CACGAACTGG CCACCAGCAG GCAAGAGCTG 
CAGGTCCTCC ATGGCAACCT GGAAACCTCT GCCCAGTCTG AGGCGAAATG GCTAACGCAG 
ATCGCGGAGC TGGAGAAGGA ACAAGGCAGT TTGGCGACAG CCGCCGCTCA AAGAGAGGAA 
GAGTTCTCAG CCCTCCGAGA CCAACTGGAA AGCACCCAGC TCAAACTGGC TGGTGCCCAG 
GATTCCATGT GTCAGCAGAT AAAGGACCAG AGGAGAATCC TCTTGGCAGG GGCCAGGAAG 
GCTGCGGAGC TCGAGATACA GGAGGCCCTG GGCCAGCTTG AAGAGCCTGC CCTCCTCATC 
AGCTGTGCAG GAGCCACAGA TCATCTTCTC TCCAAAGTCA ACTCCGTTTC CAGCTGCCTC 
GAGCAACTGG AAAAGAGCCG CAGCCAGTAT CTGGCCTGCC CAGAAGAGAT CAGTGAGCTT 
CTGCACTCAA TAACCCTGCT TGGCCACTTG ACCAGTGACA CCATCATTCA GGGGACTGCC 
ACCAGCCTCC GGGCCCCACC AGAGTCAGCC GACTCTCTGA CGGAGGCCTG TAGGCAGTAT 
GGCAGAGAAA CTCTGGCCTA TCTGTCCTCC CTAGAGGAAG AGGGGGCCAT GGAAAAAGCT 
GACACCACAG CCATAGCGAA CTGCCTCGGC AAGATCAAGA CCATTGGCGA GGAACTGCTG 
CCCAGGGGCT TGGACATCAA GCAGGAAGAG CTGGGTGACC TGGTGGACAA GGAGATGGCA 
GCCACTTCAG CTGCCATTGA AGCTGCCACC ACCCGGATAG AGGAAATTCT CAGTAAGTCC 
CGAGCAGGAG ACACGGGAGT CAAGTTGGAG GTGAACGAGA GGATCCTTGG CTCCTGTACC 
AGCCTGATGC AAGCTATCAA GGTGCTCGTT GTGGCCTCCA AGGACCTCCA GAAGGAGATA 
GTGGAAAGCG GCAGGGGTAC CGCATCCCCT AAAGAATTTT ACGCCAAGAA CTCTCGGTGG 
ACAGAAGGGC TCATATCTGC CTCCAAAGCT GTTGGTTGGG GAGCTACCAT CATGGTGGAT 
GCTGCTGACC TTGTGGTCCA AGGCAAAGGG AAGTTCGAGG AGCTGATGGT GTGCTCGCAT 
GAGATTGCCG CTAGCACAGC CCAGCTTGTG GCTGCATCCA AGGTGAAAGC GAACAAGGGC 
AGCCTCAATC TGACCCAGCT GCAGCAGGCC TCTCGGGGAG TGAACCAGGC CACGGCCGCT 
GTGGTGGCCT CAACCATTTC CGGCAAATCT CAAATTGAGG AAACAGACAG TATGGACTTC 
TCAAGCATGA CACTGACCCA GATCAAGCGC CAGGAAATGG ATTCCCAGGT TAGGGTGCTG 
GAGCTAGAAA ACGACCTGCA GAAGGAGCGC CAGAAACTAG GAGAGCTTCG GAAAAAACAC 
TACGAGCTGG CTGGTGTTGC TGAGGGCTGG GAAGAAGGGA CAGAAGCATC ACCTTCTGCT 
GTTCAAGAAG CGGTGCCGGA CAAAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453742.1
CDS	773..3922
Translation	MPGSKACSLGTAWDSWSGSAAHLCATPSPKRGTREDPEGPWMVSWPSSIPRTVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARVSEQTQRKTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	XM_017598253.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598253.1

ORF Insert Sequence:

ATGCCAGGCA GCAAAGCCTG CTCCCTTGGG ACAGCTTGGG ACAGCTGGTC TGGGAGTGCT 
GCCCACCTCT GCGCTACCCC CTCTCCCAAG CGAGGTACTC GAGAGGACCC AGAGGGCCCT 
TGGATGGTCT CTTGGCCCTC GTCAATACCA AGGACGGTCA GCGTCAATAA GGCCATTAAT 
ACGCAGGAAG TGGCCGTGAA GGAAAAACAT GCCAGGACGT GTATTCTGGG CACCCACCAT 
GAGAAAGGGG CGCAGACCTT CTGGTCTGTG GTCAACCGAC TGCCTCTCTC CAGCAACGCC 
ATGCTGTGCT GGAAGTTCTG CCACGTGTTC CACAAACTCC TTCGAGACGG ACATCCGAAC 
GTGCTGAAAG ACTCTCTCAG ATACAAAAAT GAGTTAAGCG ACATGAGCAG GATGTGGGGC 
CACCTGAGTG AGGGGTATGG ACAGCTCTGC AGCATCTACC TCAAACTGCT GAGAACAAGG 
ATGGAGTACC ACACCAAAAA TCCCAGGTTC CCAGGTAACC TCCAGATGAG CGACCGGCAG 
CTGGATGAAG CTGGGGAAAG TGATGTTAAC AACTTTTTCC AGCTCACAGT GGAGATGTTT 
GATTACCTGG AGTGCGAACT GAACCTCTTT CAAACTGTGT TCAACTCCTT GGACATGTCC 
CGCTCAGTGT CCGTGACCAC AGCTGGCCAG TGCCGCCTCG CCCCGCTCAT CCAGGTCATC 
CTGGACTGCA GTCACCTCTA TGACTACACT GTCAAGCTGC TCTTCAAGCT CCACTCCTGT 
CTTCCAGCTG ACACCCTGCA GGGCCACCGA GACCGCTTCA TGGAGCAGTT CACAAAGTTG 
AAAGATCTGT TTCAGCGCTC CAGCAACCTT CAGTACTTCA AGCGGCTCAT TCAGATCCCC 
CAGTTGCCTG AGAATCCACC CAACTTCCTA CGAGCCTCGG CCCTGTCCGA GCACATCAGT 
CCTGTGGTGG TGATCCCGGC TGAGGTGTCG TCTCCAGACA GTGAGCCCGT CCTGGAGAAG 
GATGACCTCA TGGACATGGA TGCCTCCCAG CAGAGTTTGT TTGACAACAA GTTTGATGAC 
ATCTTCGGCA GCTCGTTGAG CAGTGACCCC TTCAATTTCA ACAATCAAAA TGGCGTGAAC 
AAGGATGAGA AGGACCACTT GATTGAACGC CTCTACAGAG AGATCAGTGG ACTGACAGGA 
CAGCTGGACA ACGTGAAGAT TGAGAGCCAG CGGGCCATGC TGCAGCTGAA GGGTCGCGTG 
AGTGAGCTGG AAGCAGAGCT GGCCGAGCAG CAGCACCTGG GACGGCAGGC TACGGATGAC 
TGCGAGTTCC TGCGCACGGA GCTGGATGAA CTGAAGAGGC AGCGAGAGGA CACTGAGAAG 
GCGCAGCGCA GCCTGACTGA GATAGAAAAA AAGGCCCAGG CCAATGAACA GCGGTATAGC 
AAGTTAAAAG AGAAGTACAG TGAACTGGTG CAGAACCATG CTGATCTGCT GCGGAAGAAT 
GCAGAGGTGA CCAAGCAGGT GTCTGTGGCC CGGCAAGCCC AGGTGGATTT GGAAAGAGAG 
AAAAAAGAAC TAGCGGATTC CTTTGCACGT GTAAGCGAGC AGACCCAGCG GAAGACTCAA 
GAGCAACAGG ATGTTCTAGA GACCCTGAAG CACGAACTGG CCACCAGCAG GCAAGAGCTG 
CAGGTCCTCC ATGGCAACCT GGAAACCTCT GCCCAGTCTG AGGCGAAATG GCTAACGCAG 
ATCGCGGAGC TGGAGAAGGA ACAAGGCAGT TTGGCGACAG CCGCCGCTCA AAGAGAGGAA 
GAGTTCTCAG CCCTCCGAGA CCAACTGGAA AGCACCCAGC TCAAACTGGC TGGTGCCCAG 
GATTCCATGT GTCAGCAGAT AAAGGACCAG AGGAGAATCC TCTTGGCAGG GGCCAGGAAG 
GCTGCGGAGC TCGAGATACA GGAGGCCCTG GGCCAGCTTG AAGAGCCTGC CCTCCTCATC 
AGCTGTGCAG GAGCCACAGA TCATCTTCTC TCCAAAGTCA ACTCCGTTTC CAGCTGCCTC 
GAGCAACTGG AAAAGAGCCG CAGCCAGTAT CTGGCCTGCC CAGAAGAGAT CAGTGAGCTT 
CTGCACTCAA TAACCCTGCT TGGCCACTTG ACCAGTGACA CCATCATTCA GGGGACTGCC 
ACCAGCCTCC GGGCCCCACC AGAGTCAGCC GACTCTCTGA CGGAGGCCTG TAGGCAGTAT 
GGCAGAGAAA CTCTGGCCTA TCTGTCCTCC CTAGAGGAAG AGGGGGCCAT GGAAAAAGCT 
GACACCACAG CCATAGCGAA CTGCCTCGGC AAGATCAAGA CCATTGGCGA GGAACTGCTG 
CCCAGGGGCT TGGACATCAA GCAGGAAGAG CTGGGTGACC TGGTGGACAA GGAGATGGCA 
GCCACTTCAG CTGCCATTGA AGCTGCCACC ACCCGGATAG AGGAAATTCT CAGTAAGTCC 
CGAGCAGGAG ACACGGGAGT CAAGTTGGAG GTGAACGAGA GGATCCTTGG CTCCTGTACC 
AGCCTGATGC AAGCTATCAA GGTGCTCGTT GTGGCCTCCA AGGACCTCCA GAAGGAGATA 
GTGGAAAGCG GCAGGGGTAC CGCATCCCCT AAAGAATTTT ACGCCAAGAA CTCTCGGTGG 
ACAGAAGGGC TCATATCTGC CTCCAAAGCT GTTGGTTGGG GAGCTACCAT CATGGTGGAT 
GCTGCTGACC TTGTGGTCCA AGGCAAAGGG AAGTTCGAGG AGCTGATGGT GTGCTCGCAT 
GAGATTGCCG CTAGCACAGC CCAGCTTGTG GCTGCATCCA AGGTGAAAGC GAACAAGGGC 
AGCCTCAATC TGACCCAGCT GCAGCAGGCC TCTCGGGGAG TGAACCAGGC CACGGCCGCT 
GTGGTGGCCT CAACCATTTC CGGCAAATCT CAAATTGAGG AAACAGACAG TATGGACTTC 
TCAAGCATGA CACTGACCCA GATCAAGCGC CAGGAAATGG ATTCCCAGGT TAGGGTGCTG 
GAGCTAGAAA ACGACCTGCA GAAGGAGCGC CAGAAACTAG GAGAGCTTCG GAAAAAACAC 
TACGAGCTGG CTGGTGTTGC TGAGGGCTGG GAAGAAGGGA CAGAAGCATC ACCTTCTGCT 
GTTCAAGAAG CGGTGCCGGA CAAAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hip1 ( XM_017598252.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453741.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57776
Clone ID Related Accession (Same CDS sequence)	XM_017598253.1 , XM_017598252.1
Accession Version	XM_017598252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCA GCAAAGCCTG CTCCCTTGGG ACAGCTTGGG ACAGCTGGTC TGGGAGTGCT 
GCCCACCTCT GCGCTACCCC CTCTCCCAAG CGAGGTACTC GAGAGGACCC AGAGGGCCCT 
TGGATGGTCT CTTGGCCCTC GTCAATACCA AGGACGGTCA GCGTCAATAA GGCCATTAAT 
ACGCAGGAAG TGGCCGTGAA GGAAAAACAT GCCAGGACGT GTATTCTGGG CACCCACCAT 
GAGAAAGGGG CGCAGACCTT CTGGTCTGTG GTCAACCGAC TGCCTCTCTC CAGCAACGCC 
ATGCTGTGCT GGAAGTTCTG CCACGTGTTC CACAAACTCC TTCGAGACGG ACATCCGAAC 
GTGCTGAAAG ACTCTCTCAG ATACAAAAAT GAGTTAAGCG ACATGAGCAG GATGTGGGGC 
CACCTGAGTG AGGGGTATGG ACAGCTCTGC AGCATCTACC TCAAACTGCT GAGAACAAGG 
ATGGAGTACC ACACCAAAAA TCCCAGGTTC CCAGGTAACC TCCAGATGAG CGACCGGCAG 
CTGGATGAAG CTGGGGAAAG TGATGTTAAC AACTTTTTCC AGCTCACAGT GGAGATGTTT 
GATTACCTGG AGTGCGAACT GAACCTCTTT CAAACTGTGT TCAACTCCTT GGACATGTCC 
CGCTCAGTGT CCGTGACCAC AGCTGGCCAG TGCCGCCTCG CCCCGCTCAT CCAGGTCATC 
CTGGACTGCA GTCACCTCTA TGACTACACT GTCAAGCTGC TCTTCAAGCT CCACTCCTGT 
CTTCCAGCTG ACACCCTGCA GGGCCACCGA GACCGCTTCA TGGAGCAGTT CACAAAGTTG 
AAAGATCTGT TTCAGCGCTC CAGCAACCTT CAGTACTTCA AGCGGCTCAT TCAGATCCCC 
CAGTTGCCTG AGAATCCACC CAACTTCCTA CGAGCCTCGG CCCTGTCCGA GCACATCAGT 
CCTGTGGTGG TGATCCCGGC TGAGGTGTCG TCTCCAGACA GTGAGCCCGT CCTGGAGAAG 
GATGACCTCA TGGACATGGA TGCCTCCCAG CAGAGTTTGT TTGACAACAA GTTTGATGAC 
ATCTTCGGCA GCTCGTTGAG CAGTGACCCC TTCAATTTCA ACAATCAAAA TGGCGTGAAC 
AAGGATGAGA AGGACCACTT GATTGAACGC CTCTACAGAG AGATCAGTGG ACTGACAGGA 
CAGCTGGACA ACGTGAAGAT TGAGAGCCAG CGGGCCATGC TGCAGCTGAA GGGTCGCGTG 
AGTGAGCTGG AAGCAGAGCT GGCCGAGCAG CAGCACCTGG GACGGCAGGC TACGGATGAC 
TGCGAGTTCC TGCGCACGGA GCTGGATGAA CTGAAGAGGC AGCGAGAGGA CACTGAGAAG 
GCGCAGCGCA GCCTGACTGA GATAGAAAAA AAGGCCCAGG CCAATGAACA GCGGTATAGC 
AAGTTAAAAG AGAAGTACAG TGAACTGGTG CAGAACCATG CTGATCTGCT GCGGAAGAAT 
GCAGAGGTGA CCAAGCAGGT GTCTGTGGCC CGGCAAGCCC AGGTGGATTT GGAAAGAGAG 
AAAAAAGAAC TAGCGGATTC CTTTGCACGT GTAAGCGAGC AGACCCAGCG GAAGACTCAA 
GAGCAACAGG ATGTTCTAGA GACCCTGAAG CACGAACTGG CCACCAGCAG GCAAGAGCTG 
CAGGTCCTCC ATGGCAACCT GGAAACCTCT GCCCAGTCTG AGGCGAAATG GCTAACGCAG 
ATCGCGGAGC TGGAGAAGGA ACAAGGCAGT TTGGCGACAG CCGCCGCTCA AAGAGAGGAA 
GAGTTCTCAG CCCTCCGAGA CCAACTGGAA AGCACCCAGC TCAAACTGGC TGGTGCCCAG 
GATTCCATGT GTCAGCAGAT AAAGGACCAG AGGAGAATCC TCTTGGCAGG GGCCAGGAAG 
GCTGCGGAGC TCGAGATACA GGAGGCCCTG GGCCAGCTTG AAGAGCCTGC CCTCCTCATC 
AGCTGTGCAG GAGCCACAGA TCATCTTCTC TCCAAAGTCA ACTCCGTTTC CAGCTGCCTC 
GAGCAACTGG AAAAGAGCCG CAGCCAGTAT CTGGCCTGCC CAGAAGAGAT CAGTGAGCTT 
CTGCACTCAA TAACCCTGCT TGGCCACTTG ACCAGTGACA CCATCATTCA GGGGACTGCC 
ACCAGCCTCC GGGCCCCACC AGAGTCAGCC GACTCTCTGA CGGAGGCCTG TAGGCAGTAT 
GGCAGAGAAA CTCTGGCCTA TCTGTCCTCC CTAGAGGAAG AGGGGGCCAT GGAAAAAGCT 
GACACCACAG CCATAGCGAA CTGCCTCGGC AAGATCAAGA CCATTGGCGA GGAACTGCTG 
CCCAGGGGCT TGGACATCAA GCAGGAAGAG CTGGGTGACC TGGTGGACAA GGAGATGGCA 
GCCACTTCAG CTGCCATTGA AGCTGCCACC ACCCGGATAG AGGAAATTCT CAGTAAGTCC 
CGAGCAGGAG ACACGGGAGT CAAGTTGGAG GTGAACGAGA GGATCCTTGG CTCCTGTACC 
AGCCTGATGC AAGCTATCAA GGTGCTCGTT GTGGCCTCCA AGGACCTCCA GAAGGAGATA 
GTGGAAAGCG GCAGGGGTAC CGCATCCCCT AAAGAATTTT ACGCCAAGAA CTCTCGGTGG 
ACAGAAGGGC TCATATCTGC CTCCAAAGCT GTTGGTTGGG GAGCTACCAT CATGGTGGAT 
GCTGCTGACC TTGTGGTCCA AGGCAAAGGG AAGTTCGAGG AGCTGATGGT GTGCTCGCAT 
GAGATTGCCG CTAGCACAGC CCAGCTTGTG GCTGCATCCA AGGTGAAAGC GAACAAGGGC 
AGCCTCAATC TGACCCAGCT GCAGCAGGCC TCTCGGGGAG TGAACCAGGC CACGGCCGCT 
GTGGTGGCCT CAACCATTTC CGGCAAATCT CAAATTGAGG AAACAGACAG TATGGACTTC 
TCAAGCATGA CACTGACCCA GATCAAGCGC CAGGAAATGG ATTCCCAGGT TAGGGTGCTG 
GAGCTAGAAA ACGACCTGCA GAAGGAGCGC CAGAAACTAG GAGAGCTTCG GAAAAAACAC 
TACGAGCTGG CTGGTGTTGC TGAGGGCTGG GAAGAAGGGA CAGAAGCATC ACCTTCTGCT 
GTTCAAGAAG CGGTGCCGGA CAAAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453741.1
CDS	773..3922
Translation	MPGSKACSLGTAWDSWSGSAAHLCATPSPKRGTREDPEGPWMVSWPSSIPRTVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARVSEQTQRKTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	XM_017598252.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598252.1

ORF Insert Sequence:

ATGCCAGGCA GCAAAGCCTG CTCCCTTGGG ACAGCTTGGG ACAGCTGGTC TGGGAGTGCT 
GCCCACCTCT GCGCTACCCC CTCTCCCAAG CGAGGTACTC GAGAGGACCC AGAGGGCCCT 
TGGATGGTCT CTTGGCCCTC GTCAATACCA AGGACGGTCA GCGTCAATAA GGCCATTAAT 
ACGCAGGAAG TGGCCGTGAA GGAAAAACAT GCCAGGACGT GTATTCTGGG CACCCACCAT 
GAGAAAGGGG CGCAGACCTT CTGGTCTGTG GTCAACCGAC TGCCTCTCTC CAGCAACGCC 
ATGCTGTGCT GGAAGTTCTG CCACGTGTTC CACAAACTCC TTCGAGACGG ACATCCGAAC 
GTGCTGAAAG ACTCTCTCAG ATACAAAAAT GAGTTAAGCG ACATGAGCAG GATGTGGGGC 
CACCTGAGTG AGGGGTATGG ACAGCTCTGC AGCATCTACC TCAAACTGCT GAGAACAAGG 
ATGGAGTACC ACACCAAAAA TCCCAGGTTC CCAGGTAACC TCCAGATGAG CGACCGGCAG 
CTGGATGAAG CTGGGGAAAG TGATGTTAAC AACTTTTTCC AGCTCACAGT GGAGATGTTT 
GATTACCTGG AGTGCGAACT GAACCTCTTT CAAACTGTGT TCAACTCCTT GGACATGTCC 
CGCTCAGTGT CCGTGACCAC AGCTGGCCAG TGCCGCCTCG CCCCGCTCAT CCAGGTCATC 
CTGGACTGCA GTCACCTCTA TGACTACACT GTCAAGCTGC TCTTCAAGCT CCACTCCTGT 
CTTCCAGCTG ACACCCTGCA GGGCCACCGA GACCGCTTCA TGGAGCAGTT CACAAAGTTG 
AAAGATCTGT TTCAGCGCTC CAGCAACCTT CAGTACTTCA AGCGGCTCAT TCAGATCCCC 
CAGTTGCCTG AGAATCCACC CAACTTCCTA CGAGCCTCGG CCCTGTCCGA GCACATCAGT 
CCTGTGGTGG TGATCCCGGC TGAGGTGTCG TCTCCAGACA GTGAGCCCGT CCTGGAGAAG 
GATGACCTCA TGGACATGGA TGCCTCCCAG CAGAGTTTGT TTGACAACAA GTTTGATGAC 
ATCTTCGGCA GCTCGTTGAG CAGTGACCCC TTCAATTTCA ACAATCAAAA TGGCGTGAAC 
AAGGATGAGA AGGACCACTT GATTGAACGC CTCTACAGAG AGATCAGTGG ACTGACAGGA 
CAGCTGGACA ACGTGAAGAT TGAGAGCCAG CGGGCCATGC TGCAGCTGAA GGGTCGCGTG 
AGTGAGCTGG AAGCAGAGCT GGCCGAGCAG CAGCACCTGG GACGGCAGGC TACGGATGAC 
TGCGAGTTCC TGCGCACGGA GCTGGATGAA CTGAAGAGGC AGCGAGAGGA CACTGAGAAG 
GCGCAGCGCA GCCTGACTGA GATAGAAAAA AAGGCCCAGG CCAATGAACA GCGGTATAGC 
AAGTTAAAAG AGAAGTACAG TGAACTGGTG CAGAACCATG CTGATCTGCT GCGGAAGAAT 
GCAGAGGTGA CCAAGCAGGT GTCTGTGGCC CGGCAAGCCC AGGTGGATTT GGAAAGAGAG 
AAAAAAGAAC TAGCGGATTC CTTTGCACGT GTAAGCGAGC AGACCCAGCG GAAGACTCAA 
GAGCAACAGG ATGTTCTAGA GACCCTGAAG CACGAACTGG CCACCAGCAG GCAAGAGCTG 
CAGGTCCTCC ATGGCAACCT GGAAACCTCT GCCCAGTCTG AGGCGAAATG GCTAACGCAG 
ATCGCGGAGC TGGAGAAGGA ACAAGGCAGT TTGGCGACAG CCGCCGCTCA AAGAGAGGAA 
GAGTTCTCAG CCCTCCGAGA CCAACTGGAA AGCACCCAGC TCAAACTGGC TGGTGCCCAG 
GATTCCATGT GTCAGCAGAT AAAGGACCAG AGGAGAATCC TCTTGGCAGG GGCCAGGAAG 
GCTGCGGAGC TCGAGATACA GGAGGCCCTG GGCCAGCTTG AAGAGCCTGC CCTCCTCATC 
AGCTGTGCAG GAGCCACAGA TCATCTTCTC TCCAAAGTCA ACTCCGTTTC CAGCTGCCTC 
GAGCAACTGG AAAAGAGCCG CAGCCAGTAT CTGGCCTGCC CAGAAGAGAT CAGTGAGCTT 
CTGCACTCAA TAACCCTGCT TGGCCACTTG ACCAGTGACA CCATCATTCA GGGGACTGCC 
ACCAGCCTCC GGGCCCCACC AGAGTCAGCC GACTCTCTGA CGGAGGCCTG TAGGCAGTAT 
GGCAGAGAAA CTCTGGCCTA TCTGTCCTCC CTAGAGGAAG AGGGGGCCAT GGAAAAAGCT 
GACACCACAG CCATAGCGAA CTGCCTCGGC AAGATCAAGA CCATTGGCGA GGAACTGCTG 
CCCAGGGGCT TGGACATCAA GCAGGAAGAG CTGGGTGACC TGGTGGACAA GGAGATGGCA 
GCCACTTCAG CTGCCATTGA AGCTGCCACC ACCCGGATAG AGGAAATTCT CAGTAAGTCC 
CGAGCAGGAG ACACGGGAGT CAAGTTGGAG GTGAACGAGA GGATCCTTGG CTCCTGTACC 
AGCCTGATGC AAGCTATCAA GGTGCTCGTT GTGGCCTCCA AGGACCTCCA GAAGGAGATA 
GTGGAAAGCG GCAGGGGTAC CGCATCCCCT AAAGAATTTT ACGCCAAGAA CTCTCGGTGG 
ACAGAAGGGC TCATATCTGC CTCCAAAGCT GTTGGTTGGG GAGCTACCAT CATGGTGGAT 
GCTGCTGACC TTGTGGTCCA AGGCAAAGGG AAGTTCGAGG AGCTGATGGT GTGCTCGCAT 
GAGATTGCCG CTAGCACAGC CCAGCTTGTG GCTGCATCCA AGGTGAAAGC GAACAAGGGC 
AGCCTCAATC TGACCCAGCT GCAGCAGGCC TCTCGGGGAG TGAACCAGGC CACGGCCGCT 
GTGGTGGCCT CAACCATTTC CGGCAAATCT CAAATTGAGG AAACAGACAG TATGGACTTC 
TCAAGCATGA CACTGACCCA GATCAAGCGC CAGGAAATGG ATTCCCAGGT TAGGGTGCTG 
GAGCTAGAAA ACGACCTGCA GAAGGAGCGC CAGAAACTAG GAGAGCTTCG GAAAAAACAC 
TACGAGCTGG CTGGTGTTGC TGAGGGCTGG GAAGAAGGGA CAGAAGCATC ACCTTCTGCT 
GTTCAAGAAG CGGTGCCGGA CAAAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hip1 ( NM_001100475.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001093945.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), mRNA.

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CloneID	ORa11317
Clone ID Related Accession (Same CDS sequence)	NM_001100475.1
Accession Version	NM_001100475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473973.1. On or before Nov 28, 2008 this sequence version replaced XM_347168.3, XM_001061381.1, XM_001071106.1, XM_001071073.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACCGGA TGGCCAGCTC CATGAAGCAG GTACCCAACC CGCTGCCCAA AGTGTTGAGC 
CGGCGCGGGG TTGGTGCTGG GATGGAGGCT GCGGAGCGCG AGAGCTTCGA GCGGACTCAG 
ACGGTCAGCG TCAATAAGGC CATTAATACG CAGGAAGTGG CCGTGAAGGA AAAACATGCC 
AGGACGTGTA TTCTGGGCAC CCACCATGAG AAAGGGGCGC AGACCTTCTG GTCTGTGGTC 
AACCGACTGC CTCTCTCCAG CAACGCCATG CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC 
AAACTCCTTC GAGACGGACA TCCGAACGTG CTGAAAGACT CTCTCAGATA CAAAAATGAG 
TTAAGCGACA TGAGCAGGAT GTGGGGCCAC CTGAGTGAGG GGTATGGACA GCTCTGCAGC 
ATCTACCTCA AACTGCTGAG AACAAGGATG GAGTACCACA CCAAAAATCC CAGGTTCCCA 
GGTAACCTCC AGATGAGCGA CCGGCAGCTG GATGAAGCTG GGGAAAGTGA TGTTAACAAC 
TTTTTCCAGC TCACAGTGGA GATGTTTGAT TACCTGGAGT GCGAACTGAA CCTCTTTCAA 
ACTGTGTTCA ACTCCTTGGA CATGTCCCGC TCAGTGTCCG TGACCACAGC TGGCCAGTGC 
CGCCTCGCCC CGCTCATCCA GGTCATCCTG GACTGCAGTC ACCTCTATGA CTACACTGTC 
AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT CCAGCTGACA CCCTGCAGGG CCACCGAGAC 
CGCTTCATGG AGCAGTTCAC AAAGTTGAAA GATCTGTTTC AGCGCTCCAG CAACCTTCAG 
TACTTCAAGC GGCTCATTCA GATCCCCCAG TTGCCTGAGA ATCCACCCAA CTTCCTACGA 
GCCTCGGCCC TGTCCGAGCA CATCAGTCCT GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT 
CCAGACAGTG AGCCCGTCCT GGAGAAGGAT GACCTCATGG ACATGGATGC CTCCCAGCAG 
AGTTTGTTTG ACAACAAGTT TGATGACATC TTCGGCAGCT CGTTGAGCAG TGACCCCTTC 
AATTTCAACA ATCAAAATGG CGTGAACAAG GATGAGAAGG ACCACTTGAT TGAACGCCTC 
TACAGAGAGA TCAGTGGACT GACAGGACAG CTGGACAACG TGAAGATTGA GAGCCAGCGG 
GCCATGCTGC AGCTGAAGGG TCGCGTGAGT GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG 
CACCTGGGAC GGCAGGCTAC GGATGACTGC GAGTTCCTGC GCACGGAGCT GGATGAACTG 
AAGAGGCAGC GAGAGGACAC TGAGAAGGCG CAGCGCAGCC TGACTGAGAT AGAAAAAAAG 
GCCCAGGCCA ATGAACAGCG GTATAGCAAG TTAAAAGAGA AGTACAGTGA ACTGGTGCAG 
AACCATGCTG ATCTGCTGCG GAAGAATGCA GAGGTGACCA AGCAGGTGTC TGTGGCCCGG 
CAAGCCCAGG TGGATTTGGA AAGAGAGAAA AAAGAACTAG CGGATTCCTT TGCACGTACT 
CAAGAGCAAC AGGATGTTCT AGAGACCCTG AAGCACGAAC TGGCCACCAG CAGGCAAGAG 
CTGCAGGTCC TCCATGGCAA CCTGGAAACC TCTGCCCAGT CTGAGGCGAA ATGGCTAACG 
CAGATCGCGG AGCTGGAGAA GGAACAAGGC AGTTTGGCGA CAGCCGCCGC TCAAAGAGAG 
GAAGAGTTCT CAGCCCTCCG AGACCAACTG GAAAGCACCC AGCTCAAACT GGCTGGTGCC 
CAGGATTCCA TGTGTCAGCA GATAAAGGAC CAGAGGAGAA TCCTCTTGGC AGGGGCCAGG 
AAGGCTGCGG AGCTCGAGAT ACAGGAGGCC CTGGGCCAGC TTGAAGAGCC TGCCCTCCTC 
ATCAGCTGTG CAGGAGCCAC AGATCATCTT CTCTCCAAAG TCAACTCCGT TTCCAGCTGC 
CTCGAGCAAC TGGAAAAGAG CCGCAGCCAG TATCTGGCCT GCCCAGAAGA GATCAGTGAG 
CTTCTGCACT CAATAACCCT GCTTGGCCAC TTGACCAGTG ACACCATCAT TCAGGGGACT 
GCCACCAGCC TCCGGGCCCC ACCAGAGTCA GCCGACTCTC TGACGGAGGC CTGTAGGCAG 
TATGGCAGAG AAACTCTGGC CTATCTGTCC TCCCTAGAGG AAGAGGGGGC CATGGAAAAA 
GCTGACACCA CAGCCATAGC GAACTGCCTC GGCAAGATCA AGACCATTGG CGAGGAACTG 
CTGCCCAGGG GCTTGGACAT CAAGCAGGAA GAGCTGGGTG ACCTGGTGGA CAAGGAGATG 
GCAGCCACTT CAGCTGCCAT TGAAGCTGCC ACCACCCGGA TAGAGGAAAT TCTCAGTAAG 
TCCCGAGCAG GAGACACGGG AGTCAAGTTG GAGGTGAACG AGAGGATCCT TGGCTCCTGT 
ACCAGCCTGA TGCAAGCTAT CAAGGTGCTC GTTGTGGCCT CCAAGGACCT CCAGAAGGAG 
ATAGTGGAAA GCGGCAGGGG TACCGCATCC CCTAAAGAAT TTTACGCCAA GAACTCTCGG 
TGGACAGAAG GGCTCATATC TGCCTCCAAA GCTGTTGGTT GGGGAGCTAC CATCATGGTG 
GATGCTGCTG ACCTTGTGGT CCAAGGCAAA GGGAAGTTCG AGGAGCTGAT GGTGTGCTCG 
CATGAGATTG CCGCTAGCAC AGCCCAGCTT GTGGCTGCAT CCAAGGTGAA AGCGAACAAG 
GGCAGCCTCA ATCTGACCCA GCTGCAGCAG GCCTCTCGGG GAGTGAACCA GGCCACGGCC 
GCTGTGGTGG CCTCAACCAT TTCCGGCAAA TCTCAAATTG AGGAAACAGA CAGTATGGAC 
TTCTCAAGCA TGACACTGAC CCAGATCAAG CGCCAGGAAA TGGATTCCCA GGTTAGGGTG 
CTGGAGCTAG AAAACGACCT GCAGAAGGAG CGCCAGAAAC TAGGAGAGCT TCGGAAAAAA 
CACTACGAGC TGGCTGGTGT TGCTGAGGGC TGGGAAGAAG GGACAGAAGC ATCACCTTCT 
GCTGTTCAAG AAGCGGTGCC GGACAAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093945.1
CDS	65..3157
Translation	MDRMASSMKQVPNPLPKVLSRRGVGAGMEAAERESFERTQTVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	NM_001100475.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100475.1

ORF Insert Sequence:

ATGGACCGGA TGGCCAGCTC CATGAAGCAG GTACCCAACC CGCTGCCCAA AGTGTTGAGC 
CGGCGCGGGG TTGGTGCTGG GATGGAGGCT GCGGAGCGCG AGAGCTTCGA GCGGACTCAG 
ACGGTCAGCG TCAATAAGGC CATTAATACG CAGGAAGTGG CCGTGAAGGA AAAACATGCC 
AGGACGTGTA TTCTGGGCAC CCACCATGAG AAAGGGGCGC AGACCTTCTG GTCTGTGGTC 
AACCGACTGC CTCTCTCCAG CAACGCCATG CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC 
AAACTCCTTC GAGACGGACA TCCGAACGTG CTGAAAGACT CTCTCAGATA CAAAAATGAG 
TTAAGCGACA TGAGCAGGAT GTGGGGCCAC CTGAGTGAGG GGTATGGACA GCTCTGCAGC 
ATCTACCTCA AACTGCTGAG AACAAGGATG GAGTACCACA CCAAAAATCC CAGGTTCCCA 
GGTAACCTCC AGATGAGCGA CCGGCAGCTG GATGAAGCTG GGGAAAGTGA TGTTAACAAC 
TTTTTCCAGC TCACAGTGGA GATGTTTGAT TACCTGGAGT GCGAACTGAA CCTCTTTCAA 
ACTGTGTTCA ACTCCTTGGA CATGTCCCGC TCAGTGTCCG TGACCACAGC TGGCCAGTGC 
CGCCTCGCCC CGCTCATCCA GGTCATCCTG GACTGCAGTC ACCTCTATGA CTACACTGTC 
AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT CCAGCTGACA CCCTGCAGGG CCACCGAGAC 
CGCTTCATGG AGCAGTTCAC AAAGTTGAAA GATCTGTTTC AGCGCTCCAG CAACCTTCAG 
TACTTCAAGC GGCTCATTCA GATCCCCCAG TTGCCTGAGA ATCCACCCAA CTTCCTACGA 
GCCTCGGCCC TGTCCGAGCA CATCAGTCCT GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT 
CCAGACAGTG AGCCCGTCCT GGAGAAGGAT GACCTCATGG ACATGGATGC CTCCCAGCAG 
AGTTTGTTTG ACAACAAGTT TGATGACATC TTCGGCAGCT CGTTGAGCAG TGACCCCTTC 
AATTTCAACA ATCAAAATGG CGTGAACAAG GATGAGAAGG ACCACTTGAT TGAACGCCTC 
TACAGAGAGA TCAGTGGACT GACAGGACAG CTGGACAACG TGAAGATTGA GAGCCAGCGG 
GCCATGCTGC AGCTGAAGGG TCGCGTGAGT GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG 
CACCTGGGAC GGCAGGCTAC GGATGACTGC GAGTTCCTGC GCACGGAGCT GGATGAACTG 
AAGAGGCAGC GAGAGGACAC TGAGAAGGCG CAGCGCAGCC TGACTGAGAT AGAAAAAAAG 
GCCCAGGCCA ATGAACAGCG GTATAGCAAG TTAAAAGAGA AGTACAGTGA ACTGGTGCAG 
AACCATGCTG ATCTGCTGCG GAAGAATGCA GAGGTGACCA AGCAGGTGTC TGTGGCCCGG 
CAAGCCCAGG TGGATTTGGA AAGAGAGAAA AAAGAACTAG CGGATTCCTT TGCACGTACT 
CAAGAGCAAC AGGATGTTCT AGAGACCCTG AAGCACGAAC TGGCCACCAG CAGGCAAGAG 
CTGCAGGTCC TCCATGGCAA CCTGGAAACC TCTGCCCAGT CTGAGGCGAA ATGGCTAACG 
CAGATCGCGG AGCTGGAGAA GGAACAAGGC AGTTTGGCGA CAGCCGCCGC TCAAAGAGAG 
GAAGAGTTCT CAGCCCTCCG AGACCAACTG GAAAGCACCC AGCTCAAACT GGCTGGTGCC 
CAGGATTCCA TGTGTCAGCA GATAAAGGAC CAGAGGAGAA TCCTCTTGGC AGGGGCCAGG 
AAGGCTGCGG AGCTCGAGAT ACAGGAGGCC CTGGGCCAGC TTGAAGAGCC TGCCCTCCTC 
ATCAGCTGTG CAGGAGCCAC AGATCATCTT CTCTCCAAAG TCAACTCCGT TTCCAGCTGC 
CTCGAGCAAC TGGAAAAGAG CCGCAGCCAG TATCTGGCCT GCCCAGAAGA GATCAGTGAG 
CTTCTGCACT CAATAACCCT GCTTGGCCAC TTGACCAGTG ACACCATCAT TCAGGGGACT 
GCCACCAGCC TCCGGGCCCC ACCAGAGTCA GCCGACTCTC TGACGGAGGC CTGTAGGCAG 
TATGGCAGAG AAACTCTGGC CTATCTGTCC TCCCTAGAGG AAGAGGGGGC CATGGAAAAA 
GCTGACACCA CAGCCATAGC GAACTGCCTC GGCAAGATCA AGACCATTGG CGAGGAACTG 
CTGCCCAGGG GCTTGGACAT CAAGCAGGAA GAGCTGGGTG ACCTGGTGGA CAAGGAGATG 
GCAGCCACTT CAGCTGCCAT TGAAGCTGCC ACCACCCGGA TAGAGGAAAT TCTCAGTAAG 
TCCCGAGCAG GAGACACGGG AGTCAAGTTG GAGGTGAACG AGAGGATCCT TGGCTCCTGT 
ACCAGCCTGA TGCAAGCTAT CAAGGTGCTC GTTGTGGCCT CCAAGGACCT CCAGAAGGAG 
ATAGTGGAAA GCGGCAGGGG TACCGCATCC CCTAAAGAAT TTTACGCCAA GAACTCTCGG 
TGGACAGAAG GGCTCATATC TGCCTCCAAA GCTGTTGGTT GGGGAGCTAC CATCATGGTG 
GATGCTGCTG ACCTTGTGGT CCAAGGCAAA GGGAAGTTCG AGGAGCTGAT GGTGTGCTCG 
CATGAGATTG CCGCTAGCAC AGCCCAGCTT GTGGCTGCAT CCAAGGTGAA AGCGAACAAG 
GGCAGCCTCA ATCTGACCCA GCTGCAGCAG GCCTCTCGGG GAGTGAACCA GGCCACGGCC 
GCTGTGGTGG CCTCAACCAT TTCCGGCAAA TCTCAAATTG AGGAAACAGA CAGTATGGAC 
TTCTCAAGCA TGACACTGAC CCAGATCAAG CGCCAGGAAA TGGATTCCCA GGTTAGGGTG 
CTGGAGCTAG AAAACGACCT GCAGAAGGAG CGCCAGAAAC TAGGAGAGCT TCGGAAAAAA 
CACTACGAGC TGGCTGGTGT TGCTGAGGGC TGGGAAGAAG GGACAGAAGC ATCACCTTCT 
GCTGTTCAAG AAGCGGTGCC GGACAAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hip1 ( XM_006249098.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249160.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X3, mRNA.

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CloneID	ORa34730
Clone ID Related Accession (Same CDS sequence)	XM_006249098.3
Accession Version	XM_006249098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGA TGGCCAGCTC CATGAAGCAG GTACCCAACC CGCTGCCCAA AGTGTTGAGC 
CGGCGCGGGG TTGGTGCTGG GATGGAGGCT GCGGAGCGCG AGAGCTTCGA GCGGACTCAG 
ACGGTCAGCG TCAATAAGGC CATTAATACG CAGGAAGTGG CCGTGAAGGA AAAACATGCC 
AGGACGTGTA TTCTGGGCAC CCACCATGAG AAAGGGGCGC AGACCTTCTG GTCTGTGGTC 
AACCGACTGC CTCTCTCCAG CAACGCCATG CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC 
AAACTCCTTC GAGACGGACA TCCGAACGTG CTGAAAGACT CTCTCAGATA CAAAAATGAG 
TTAAGCGACA TGAGCAGGAT GTGGGGCCAC CTGAGTGAGG GGTATGGACA GCTCTGCAGC 
ATCTACCTCA AACTGCTGAG AACAAGGATG GAGTACCACA CCAAAAATCC CAGGTTCCCA 
GGTAACCTCC AGATGAGCGA CCGGCAGCTG GATGAAGCTG GGGAAAGTGA TGTTAACAAC 
TTTTTCCAGC TCACAGTGGA GATGTTTGAT TACCTGGAGT GCGAACTGAA CCTCTTTCAA 
ACTGTGTTCA ACTCCTTGGA CATGTCCCGC TCAGTGTCCG TGACCACAGC TGGCCAGTGC 
CGCCTCGCCC CGCTCATCCA GGTCATCCTG GACTGCAGTC ACCTCTATGA CTACACTGTC 
AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT CCAGCTGACA CCCTGCAGGG CCACCGAGAC 
CGCTTCATGG AGCAGTTCAC AAAGTTGAAA GATCTGTTTC AGCGCTCCAG CAACCTTCAG 
TACTTCAAGC GGCTCATTCA GATCCCCCAG TTGCCTGAGA ATCCACCCAA CTTCCTACGA 
GCCTCGGCCC TGTCCGAGCA CATCAGTCCT GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT 
CCAGACAGTG AGCCCGTCCT GGAGAAGGAT GACCTCATGG ACATGGATGC CTCCCAGCAG 
AGTTTGTTTG ACAACAAGTT TGATGACATC TTCGGCAGCT CGTTGAGCAG TGACCCCTTC 
AATTTCAACA ATCAAAATGG CGTGAACAAG GATGAGAAGG ACCACTTGAT TGAACGCCTC 
TACAGAGAGA TCAGTGGACT GACAGGACAG CTGGACAACG TGAAGATTGA GAGCCAGCGG 
GCCATGCTGC AGCTGAAGGG TCGCGTGAGT GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG 
CACCTGGGAC GGCAGGCTAC GGATGACTGC GAGTTCCTGC GCACGGAGCT GGATGAACTG 
AAGAGGCAGC GAGAGGACAC TGAGAAGGCG CAGCGCAGCC TGACTGAGAT AGAAAAAAAG 
GCCCAGGCCA ATGAACAGCG GTATAGCAAG TTAAAAGAGA AGTACAGTGA ACTGGTGCAG 
AACCATGCTG ATCTGCTGCG GAAGAATGCA GAGGTGACCA AGCAGGTGTC TGTGGCCCGG 
CAAGCCCAGG TGGATTTGGA AAGAGAGAAA AAAGAACTAG CGGATTCCTT TGCACGTGTA 
AGCGAGCAGA CCCAGCGGAA GACTCAAGAG CAACAGGATG TTCTAGAGAC CCTGAAGCAC 
GAACTGGCCA CCAGCAGGCA AGAGCTGCAG GTCCTCCATG GCAACCTGGA AACCTCTGCC 
CAGTCTGAGG CGAAATGGCT AACGCAGATC GCGGAGCTGG AGAAGGAACA AGGCAGTTTG 
GCGACAGCCG CCGCTCAAAG AGAGGAAGAG TTCTCAGCCC TCCGAGACCA ACTGGAAAGC 
ACCCAGCTCA AACTGGCTGG TGCCCAGGAT TCCATGTGTC AGCAGATAAA GGACCAGAGG 
AGAATCCTCT TGGCAGGGGC CAGGAAGGCT GCGGAGCTCG AGATACAGGA GGCCCTGGGC 
CAGCTTGAAG AGCCTGCCCT CCTCATCAGC TGTGCAGGAG CCACAGATCA TCTTCTCTCC 
AAAGTCAACT CCGTTTCCAG CTGCCTCGAG CAACTGGAAA AGAGCCGCAG CCAGTATCTG 
GCCTGCCCAG AAGAGATCAG TGAGCTTCTG CACTCAATAA CCCTGCTTGG CCACTTGACC 
AGTGACACCA TCATTCAGGG GACTGCCACC AGCCTCCGGG CCCCACCAGA GTCAGCCGAC 
TCTCTGACGG AGGCCTGTAG GCAGTATGGC AGAGAAACTC TGGCCTATCT GTCCTCCCTA 
GAGGAAGAGG GGGCCATGGA AAAAGCTGAC ACCACAGCCA TAGCGAACTG CCTCGGCAAG 
ATCAAGACCA TTGGCGAGGA ACTGCTGCCC AGGGGCTTGG ACATCAAGCA GGAAGAGCTG 
GGTGACCTGG TGGACAAGGA GATGGCAGCC ACTTCAGCTG CCATTGAAGC TGCCACCACC 
CGGATAGAGG AAATTCTCAG TAAGTCCCGA GCAGGAGACA CGGGAGTCAA GTTGGAGGTG 
AACGAGAGGA TCCTTGGCTC CTGTACCAGC CTGATGCAAG CTATCAAGGT GCTCGTTGTG 
GCCTCCAAGG ACCTCCAGAA GGAGATAGTG GAAAGCGGCA GGGGTACCGC ATCCCCTAAA 
GAATTTTACG CCAAGAACTC TCGGTGGACA GAAGGGCTCA TATCTGCCTC CAAAGCTGTT 
GGTTGGGGAG CTACCATCAT GGTGGATGCT GCTGACCTTG TGGTCCAAGG CAAAGGGAAG 
TTCGAGGAGC TGATGGTGTG CTCGCATGAG ATTGCCGCTA GCACAGCCCA GCTTGTGGCT 
GCATCCAAGG TGAAAGCGAA CAAGGGCAGC CTCAATCTGA CCCAGCTGCA GCAGGCCTCT 
CGGGGAGTGA ACCAGGCCAC GGCCGCTGTG GTGGCCTCAA CCATTTCCGG CAAATCTCAA 
ATTGAGGAAA CAGACAGTAT GGACTTCTCA AGCATGACAC TGACCCAGAT CAAGCGCCAG 
GAAATGGATT CCCAGGTTAG GGTGCTGGAG CTAGAAAACG ACCTGCAGAA GGAGCGCCAG 
AAACTAGGAG AGCTTCGGAA AAAACACTAC GAGCTGGCTG GTGTTGCTGA GGGCTGGGAA 
GAAGGGACAG AAGCATCACC TTCTGCTGTT CAAGAAGCGG TGCCGGACAA AGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249160.1
CDS	207..3323
Translation	MDRMASSMKQVPNPLPKVLSRRGVGAGMEAAERESFERTQTVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARVSEQTQRKTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	XM_006249098.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249098.3

ORF Insert Sequence:

ATGGACCGGA TGGCCAGCTC CATGAAGCAG GTACCCAACC CGCTGCCCAA AGTGTTGAGC 
CGGCGCGGGG TTGGTGCTGG GATGGAGGCT GCGGAGCGCG AGAGCTTCGA GCGGACTCAG 
ACGGTCAGCG TCAATAAGGC CATTAATACG CAGGAAGTGG CCGTGAAGGA AAAACATGCC 
AGGACGTGTA TTCTGGGCAC CCACCATGAG AAAGGGGCGC AGACCTTCTG GTCTGTGGTC 
AACCGACTGC CTCTCTCCAG CAACGCCATG CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC 
AAACTCCTTC GAGACGGACA TCCGAACGTG CTGAAAGACT CTCTCAGATA CAAAAATGAG 
TTAAGCGACA TGAGCAGGAT GTGGGGCCAC CTGAGTGAGG GGTATGGACA GCTCTGCAGC 
ATCTACCTCA AACTGCTGAG AACAAGGATG GAGTACCACA CCAAAAATCC CAGGTTCCCA 
GGTAACCTCC AGATGAGCGA CCGGCAGCTG GATGAAGCTG GGGAAAGTGA TGTTAACAAC 
TTTTTCCAGC TCACAGTGGA GATGTTTGAT TACCTGGAGT GCGAACTGAA CCTCTTTCAA 
ACTGTGTTCA ACTCCTTGGA CATGTCCCGC TCAGTGTCCG TGACCACAGC TGGCCAGTGC 
CGCCTCGCCC CGCTCATCCA GGTCATCCTG GACTGCAGTC ACCTCTATGA CTACACTGTC 
AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT CCAGCTGACA CCCTGCAGGG CCACCGAGAC 
CGCTTCATGG AGCAGTTCAC AAAGTTGAAA GATCTGTTTC AGCGCTCCAG CAACCTTCAG 
TACTTCAAGC GGCTCATTCA GATCCCCCAG TTGCCTGAGA ATCCACCCAA CTTCCTACGA 
GCCTCGGCCC TGTCCGAGCA CATCAGTCCT GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT 
CCAGACAGTG AGCCCGTCCT GGAGAAGGAT GACCTCATGG ACATGGATGC CTCCCAGCAG 
AGTTTGTTTG ACAACAAGTT TGATGACATC TTCGGCAGCT CGTTGAGCAG TGACCCCTTC 
AATTTCAACA ATCAAAATGG CGTGAACAAG GATGAGAAGG ACCACTTGAT TGAACGCCTC 
TACAGAGAGA TCAGTGGACT GACAGGACAG CTGGACAACG TGAAGATTGA GAGCCAGCGG 
GCCATGCTGC AGCTGAAGGG TCGCGTGAGT GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG 
CACCTGGGAC GGCAGGCTAC GGATGACTGC GAGTTCCTGC GCACGGAGCT GGATGAACTG 
AAGAGGCAGC GAGAGGACAC TGAGAAGGCG CAGCGCAGCC TGACTGAGAT AGAAAAAAAG 
GCCCAGGCCA ATGAACAGCG GTATAGCAAG TTAAAAGAGA AGTACAGTGA ACTGGTGCAG 
AACCATGCTG ATCTGCTGCG GAAGAATGCA GAGGTGACCA AGCAGGTGTC TGTGGCCCGG 
CAAGCCCAGG TGGATTTGGA AAGAGAGAAA AAAGAACTAG CGGATTCCTT TGCACGTGTA 
AGCGAGCAGA CCCAGCGGAA GACTCAAGAG CAACAGGATG TTCTAGAGAC CCTGAAGCAC 
GAACTGGCCA CCAGCAGGCA AGAGCTGCAG GTCCTCCATG GCAACCTGGA AACCTCTGCC 
CAGTCTGAGG CGAAATGGCT AACGCAGATC GCGGAGCTGG AGAAGGAACA AGGCAGTTTG 
GCGACAGCCG CCGCTCAAAG AGAGGAAGAG TTCTCAGCCC TCCGAGACCA ACTGGAAAGC 
ACCCAGCTCA AACTGGCTGG TGCCCAGGAT TCCATGTGTC AGCAGATAAA GGACCAGAGG 
AGAATCCTCT TGGCAGGGGC CAGGAAGGCT GCGGAGCTCG AGATACAGGA GGCCCTGGGC 
CAGCTTGAAG AGCCTGCCCT CCTCATCAGC TGTGCAGGAG CCACAGATCA TCTTCTCTCC 
AAAGTCAACT CCGTTTCCAG CTGCCTCGAG CAACTGGAAA AGAGCCGCAG CCAGTATCTG 
GCCTGCCCAG AAGAGATCAG TGAGCTTCTG CACTCAATAA CCCTGCTTGG CCACTTGACC 
AGTGACACCA TCATTCAGGG GACTGCCACC AGCCTCCGGG CCCCACCAGA GTCAGCCGAC 
TCTCTGACGG AGGCCTGTAG GCAGTATGGC AGAGAAACTC TGGCCTATCT GTCCTCCCTA 
GAGGAAGAGG GGGCCATGGA AAAAGCTGAC ACCACAGCCA TAGCGAACTG CCTCGGCAAG 
ATCAAGACCA TTGGCGAGGA ACTGCTGCCC AGGGGCTTGG ACATCAAGCA GGAAGAGCTG 
GGTGACCTGG TGGACAAGGA GATGGCAGCC ACTTCAGCTG CCATTGAAGC TGCCACCACC 
CGGATAGAGG AAATTCTCAG TAAGTCCCGA GCAGGAGACA CGGGAGTCAA GTTGGAGGTG 
AACGAGAGGA TCCTTGGCTC CTGTACCAGC CTGATGCAAG CTATCAAGGT GCTCGTTGTG 
GCCTCCAAGG ACCTCCAGAA GGAGATAGTG GAAAGCGGCA GGGGTACCGC ATCCCCTAAA 
GAATTTTACG CCAAGAACTC TCGGTGGACA GAAGGGCTCA TATCTGCCTC CAAAGCTGTT 
GGTTGGGGAG CTACCATCAT GGTGGATGCT GCTGACCTTG TGGTCCAAGG CAAAGGGAAG 
TTCGAGGAGC TGATGGTGTG CTCGCATGAG ATTGCCGCTA GCACAGCCCA GCTTGTGGCT 
GCATCCAAGG TGAAAGCGAA CAAGGGCAGC CTCAATCTGA CCCAGCTGCA GCAGGCCTCT 
CGGGGAGTGA ACCAGGCCAC GGCCGCTGTG GTGGCCTCAA CCATTTCCGG CAAATCTCAA 
ATTGAGGAAA CAGACAGTAT GGACTTCTCA AGCATGACAC TGACCCAGAT CAAGCGCCAG 
GAAATGGATT CCCAGGTTAG GGTGCTGGAG CTAGAAAACG ACCTGCAGAA GGAGCGCCAG 
AAACTAGGAG AGCTTCGGAA AAAACACTAC GAGCTGGCTG GTGTTGCTGA GGGCTGGGAA 
GAAGGGACAG AAGCATCACC TTCTGCTGTT CAAGAAGCGG TGCCGGACAA AGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hip1 ( XM_006249099.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249161.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X4, mRNA.

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CloneID	ORa34731
Clone ID Related Accession (Same CDS sequence)	XM_006249099.3
Accession Version	XM_006249099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGA ACCTAGACCC CCCTCCAGAG ACGGTCAGCG TCAATAAGGC CATTAATACG 
CAGGAAGTGG CCGTGAAGGA AAAACATGCC AGGACGTGTA TTCTGGGCAC CCACCATGAG 
AAAGGGGCGC AGACCTTCTG GTCTGTGGTC AACCGACTGC CTCTCTCCAG CAACGCCATG 
CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC AAACTCCTTC GAGACGGACA TCCGAACGTG 
CTGAAAGACT CTCTCAGATA CAAAAATGAG TTAAGCGACA TGAGCAGGAT GTGGGGCCAC 
CTGAGTGAGG GGTATGGACA GCTCTGCAGC ATCTACCTCA AACTGCTGAG AACAAGGATG 
GAGTACCACA CCAAAAATCC CAGGTTCCCA GGTAACCTCC AGATGAGCGA CCGGCAGCTG 
GATGAAGCTG GGGAAAGTGA TGTTAACAAC TTTTTCCAGC TCACAGTGGA GATGTTTGAT 
TACCTGGAGT GCGAACTGAA CCTCTTTCAA ACTGTGTTCA ACTCCTTGGA CATGTCCCGC 
TCAGTGTCCG TGACCACAGC TGGCCAGTGC CGCCTCGCCC CGCTCATCCA GGTCATCCTG 
GACTGCAGTC ACCTCTATGA CTACACTGTC AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT 
CCAGCTGACA CCCTGCAGGG CCACCGAGAC CGCTTCATGG AGCAGTTCAC AAAGTTGAAA 
GATCTGTTTC AGCGCTCCAG CAACCTTCAG TACTTCAAGC GGCTCATTCA GATCCCCCAG 
TTGCCTGAGA ATCCACCCAA CTTCCTACGA GCCTCGGCCC TGTCCGAGCA CATCAGTCCT 
GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT CCAGACAGTG AGCCCGTCCT GGAGAAGGAT 
GACCTCATGG ACATGGATGC CTCCCAGCAG AGTTTGTTTG ACAACAAGTT TGATGACATC 
TTCGGCAGCT CGTTGAGCAG TGACCCCTTC AATTTCAACA ATCAAAATGG CGTGAACAAG 
GATGAGAAGG ACCACTTGAT TGAACGCCTC TACAGAGAGA TCAGTGGACT GACAGGACAG 
CTGGACAACG TGAAGATTGA GAGCCAGCGG GCCATGCTGC AGCTGAAGGG TCGCGTGAGT 
GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG CACCTGGGAC GGCAGGCTAC GGATGACTGC 
GAGTTCCTGC GCACGGAGCT GGATGAACTG AAGAGGCAGC GAGAGGACAC TGAGAAGGCG 
CAGCGCAGCC TGACTGAGAT AGAAAAAAAG GCCCAGGCCA ATGAACAGCG GTATAGCAAG 
TTAAAAGAGA AGTACAGTGA ACTGGTGCAG AACCATGCTG ATCTGCTGCG GAAGAATGCA 
GAGGTGACCA AGCAGGTGTC TGTGGCCCGG CAAGCCCAGG TGGATTTGGA AAGAGAGAAA 
AAAGAACTAG CGGATTCCTT TGCACGTGTA AGCGAGCAGA CCCAGCGGAA GACTCAAGAG 
CAACAGGATG TTCTAGAGAC CCTGAAGCAC GAACTGGCCA CCAGCAGGCA AGAGCTGCAG 
GTCCTCCATG GCAACCTGGA AACCTCTGCC CAGTCTGAGG CGAAATGGCT AACGCAGATC 
GCGGAGCTGG AGAAGGAACA AGGCAGTTTG GCGACAGCCG CCGCTCAAAG AGAGGAAGAG 
TTCTCAGCCC TCCGAGACCA ACTGGAAAGC ACCCAGCTCA AACTGGCTGG TGCCCAGGAT 
TCCATGTGTC AGCAGATAAA GGACCAGAGG AGAATCCTCT TGGCAGGGGC CAGGAAGGCT 
GCGGAGCTCG AGATACAGGA GGCCCTGGGC CAGCTTGAAG AGCCTGCCCT CCTCATCAGC 
TGTGCAGGAG CCACAGATCA TCTTCTCTCC AAAGTCAACT CCGTTTCCAG CTGCCTCGAG 
CAACTGGAAA AGAGCCGCAG CCAGTATCTG GCCTGCCCAG AAGAGATCAG TGAGCTTCTG 
CACTCAATAA CCCTGCTTGG CCACTTGACC AGTGACACCA TCATTCAGGG GACTGCCACC 
AGCCTCCGGG CCCCACCAGA GTCAGCCGAC TCTCTGACGG AGGCCTGTAG GCAGTATGGC 
AGAGAAACTC TGGCCTATCT GTCCTCCCTA GAGGAAGAGG GGGCCATGGA AAAAGCTGAC 
ACCACAGCCA TAGCGAACTG CCTCGGCAAG ATCAAGACCA TTGGCGAGGA ACTGCTGCCC 
AGGGGCTTGG ACATCAAGCA GGAAGAGCTG GGTGACCTGG TGGACAAGGA GATGGCAGCC 
ACTTCAGCTG CCATTGAAGC TGCCACCACC CGGATAGAGG AAATTCTCAG TAAGTCCCGA 
GCAGGAGACA CGGGAGTCAA GTTGGAGGTG AACGAGAGGA TCCTTGGCTC CTGTACCAGC 
CTGATGCAAG CTATCAAGGT GCTCGTTGTG GCCTCCAAGG ACCTCCAGAA GGAGATAGTG 
GAAAGCGGCA GGGGTACCGC ATCCCCTAAA GAATTTTACG CCAAGAACTC TCGGTGGACA 
GAAGGGCTCA TATCTGCCTC CAAAGCTGTT GGTTGGGGAG CTACCATCAT GGTGGATGCT 
GCTGACCTTG TGGTCCAAGG CAAAGGGAAG TTCGAGGAGC TGATGGTGTG CTCGCATGAG 
ATTGCCGCTA GCACAGCCCA GCTTGTGGCT GCATCCAAGG TGAAAGCGAA CAAGGGCAGC 
CTCAATCTGA CCCAGCTGCA GCAGGCCTCT CGGGGAGTGA ACCAGGCCAC GGCCGCTGTG 
GTGGCCTCAA CCATTTCCGG CAAATCTCAA ATTGAGGAAA CAGACAGTAT GGACTTCTCA 
AGCATGACAC TGACCCAGAT CAAGCGCCAG GAAATGGATT CCCAGGTTAG GGTGCTGGAG 
CTAGAAAACG ACCTGCAGAA GGAGCGCCAG AAACTAGGAG AGCTTCGGAA AAAACACTAC 
GAGCTGGCTG GTGTTGCTGA GGGCTGGGAA GAAGGGACAG AAGCATCACC TTCTGCTGTT 
CAAGAAGCGG TGCCGGACAA AGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249161.1
CDS	292..3318
Translation	MFSNLDPPPETVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARVSEQTQRKTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	XM_006249099.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249099.3

ORF Insert Sequence:

ATGTTCTCGA ACCTAGACCC CCCTCCAGAG ACGGTCAGCG TCAATAAGGC CATTAATACG 
CAGGAAGTGG CCGTGAAGGA AAAACATGCC AGGACGTGTA TTCTGGGCAC CCACCATGAG 
AAAGGGGCGC AGACCTTCTG GTCTGTGGTC AACCGACTGC CTCTCTCCAG CAACGCCATG 
CTGTGCTGGA AGTTCTGCCA CGTGTTCCAC AAACTCCTTC GAGACGGACA TCCGAACGTG 
CTGAAAGACT CTCTCAGATA CAAAAATGAG TTAAGCGACA TGAGCAGGAT GTGGGGCCAC 
CTGAGTGAGG GGTATGGACA GCTCTGCAGC ATCTACCTCA AACTGCTGAG AACAAGGATG 
GAGTACCACA CCAAAAATCC CAGGTTCCCA GGTAACCTCC AGATGAGCGA CCGGCAGCTG 
GATGAAGCTG GGGAAAGTGA TGTTAACAAC TTTTTCCAGC TCACAGTGGA GATGTTTGAT 
TACCTGGAGT GCGAACTGAA CCTCTTTCAA ACTGTGTTCA ACTCCTTGGA CATGTCCCGC 
TCAGTGTCCG TGACCACAGC TGGCCAGTGC CGCCTCGCCC CGCTCATCCA GGTCATCCTG 
GACTGCAGTC ACCTCTATGA CTACACTGTC AAGCTGCTCT TCAAGCTCCA CTCCTGTCTT 
CCAGCTGACA CCCTGCAGGG CCACCGAGAC CGCTTCATGG AGCAGTTCAC AAAGTTGAAA 
GATCTGTTTC AGCGCTCCAG CAACCTTCAG TACTTCAAGC GGCTCATTCA GATCCCCCAG 
TTGCCTGAGA ATCCACCCAA CTTCCTACGA GCCTCGGCCC TGTCCGAGCA CATCAGTCCT 
GTGGTGGTGA TCCCGGCTGA GGTGTCGTCT CCAGACAGTG AGCCCGTCCT GGAGAAGGAT 
GACCTCATGG ACATGGATGC CTCCCAGCAG AGTTTGTTTG ACAACAAGTT TGATGACATC 
TTCGGCAGCT CGTTGAGCAG TGACCCCTTC AATTTCAACA ATCAAAATGG CGTGAACAAG 
GATGAGAAGG ACCACTTGAT TGAACGCCTC TACAGAGAGA TCAGTGGACT GACAGGACAG 
CTGGACAACG TGAAGATTGA GAGCCAGCGG GCCATGCTGC AGCTGAAGGG TCGCGTGAGT 
GAGCTGGAAG CAGAGCTGGC CGAGCAGCAG CACCTGGGAC GGCAGGCTAC GGATGACTGC 
GAGTTCCTGC GCACGGAGCT GGATGAACTG AAGAGGCAGC GAGAGGACAC TGAGAAGGCG 
CAGCGCAGCC TGACTGAGAT AGAAAAAAAG GCCCAGGCCA ATGAACAGCG GTATAGCAAG 
TTAAAAGAGA AGTACAGTGA ACTGGTGCAG AACCATGCTG ATCTGCTGCG GAAGAATGCA 
GAGGTGACCA AGCAGGTGTC TGTGGCCCGG CAAGCCCAGG TGGATTTGGA AAGAGAGAAA 
AAAGAACTAG CGGATTCCTT TGCACGTGTA AGCGAGCAGA CCCAGCGGAA GACTCAAGAG 
CAACAGGATG TTCTAGAGAC CCTGAAGCAC GAACTGGCCA CCAGCAGGCA AGAGCTGCAG 
GTCCTCCATG GCAACCTGGA AACCTCTGCC CAGTCTGAGG CGAAATGGCT AACGCAGATC 
GCGGAGCTGG AGAAGGAACA AGGCAGTTTG GCGACAGCCG CCGCTCAAAG AGAGGAAGAG 
TTCTCAGCCC TCCGAGACCA ACTGGAAAGC ACCCAGCTCA AACTGGCTGG TGCCCAGGAT 
TCCATGTGTC AGCAGATAAA GGACCAGAGG AGAATCCTCT TGGCAGGGGC CAGGAAGGCT 
GCGGAGCTCG AGATACAGGA GGCCCTGGGC CAGCTTGAAG AGCCTGCCCT CCTCATCAGC 
TGTGCAGGAG CCACAGATCA TCTTCTCTCC AAAGTCAACT CCGTTTCCAG CTGCCTCGAG 
CAACTGGAAA AGAGCCGCAG CCAGTATCTG GCCTGCCCAG AAGAGATCAG TGAGCTTCTG 
CACTCAATAA CCCTGCTTGG CCACTTGACC AGTGACACCA TCATTCAGGG GACTGCCACC 
AGCCTCCGGG CCCCACCAGA GTCAGCCGAC TCTCTGACGG AGGCCTGTAG GCAGTATGGC 
AGAGAAACTC TGGCCTATCT GTCCTCCCTA GAGGAAGAGG GGGCCATGGA AAAAGCTGAC 
ACCACAGCCA TAGCGAACTG CCTCGGCAAG ATCAAGACCA TTGGCGAGGA ACTGCTGCCC 
AGGGGCTTGG ACATCAAGCA GGAAGAGCTG GGTGACCTGG TGGACAAGGA GATGGCAGCC 
ACTTCAGCTG CCATTGAAGC TGCCACCACC CGGATAGAGG AAATTCTCAG TAAGTCCCGA 
GCAGGAGACA CGGGAGTCAA GTTGGAGGTG AACGAGAGGA TCCTTGGCTC CTGTACCAGC 
CTGATGCAAG CTATCAAGGT GCTCGTTGTG GCCTCCAAGG ACCTCCAGAA GGAGATAGTG 
GAAAGCGGCA GGGGTACCGC ATCCCCTAAA GAATTTTACG CCAAGAACTC TCGGTGGACA 
GAAGGGCTCA TATCTGCCTC CAAAGCTGTT GGTTGGGGAG CTACCATCAT GGTGGATGCT 
GCTGACCTTG TGGTCCAAGG CAAAGGGAAG TTCGAGGAGC TGATGGTGTG CTCGCATGAG 
ATTGCCGCTA GCACAGCCCA GCTTGTGGCT GCATCCAAGG TGAAAGCGAA CAAGGGCAGC 
CTCAATCTGA CCCAGCTGCA GCAGGCCTCT CGGGGAGTGA ACCAGGCCAC GGCCGCTGTG 
GTGGCCTCAA CCATTTCCGG CAAATCTCAA ATTGAGGAAA CAGACAGTAT GGACTTCTCA 
AGCATGACAC TGACCCAGAT CAAGCGCCAG GAAATGGATT CCCAGGTTAG GGTGCTGGAG 
CTAGAAAACG ACCTGCAGAA GGAGCGCCAG AAACTAGGAG AGCTTCGGAA AAAACACTAC 
GAGCTGGCTG GTGTTGCTGA GGGCTGGGAA GAAGGGACAG AAGCATCACC TTCTGCTGTT 
CAAGAAGCGG TGCCGGACAA AGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hip1 ( XM_006249100.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249162.1 Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa34732
Clone ID Related Accession (Same CDS sequence)	XM_006249100.3
Accession Version	XM_006249100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	huntingtin-interacting protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGA ACAAGATGAC GGTCAGCGTC AATAAGGCCA TTAATACGCA GGAAGTGGCC 
GTGAAGGAAA AACATGCCAG GACGTGTATT CTGGGCACCC ACCATGAGAA AGGGGCGCAG 
ACCTTCTGGT CTGTGGTCAA CCGACTGCCT CTCTCCAGCA ACGCCATGCT GTGCTGGAAG 
TTCTGCCACG TGTTCCACAA ACTCCTTCGA GACGGACATC CGAACGTGCT GAAAGACTCT 
CTCAGATACA AAAATGAGTT AAGCGACATG AGCAGGATGT GGGGCCACCT GAGTGAGGGG 
TATGGACAGC TCTGCAGCAT CTACCTCAAA CTGCTGAGAA CAAGGATGGA GTACCACACC 
AAAAATCCCA GGTTCCCAGG TAACCTCCAG ATGAGCGACC GGCAGCTGGA TGAAGCTGGG 
GAAAGTGATG TTAACAACTT TTTCCAGCTC ACAGTGGAGA TGTTTGATTA CCTGGAGTGC 
GAACTGAACC TCTTTCAAAC TGTGTTCAAC TCCTTGGACA TGTCCCGCTC AGTGTCCGTG 
ACCACAGCTG GCCAGTGCCG CCTCGCCCCG CTCATCCAGG TCATCCTGGA CTGCAGTCAC 
CTCTATGACT ACACTGTCAA GCTGCTCTTC AAGCTCCACT CCTGTCTTCC AGCTGACACC 
CTGCAGGGCC ACCGAGACCG CTTCATGGAG CAGTTCACAA AGTTGAAAGA TCTGTTTCAG 
CGCTCCAGCA ACCTTCAGTA CTTCAAGCGG CTCATTCAGA TCCCCCAGTT GCCTGAGAAT 
CCACCCAACT TCCTACGAGC CTCGGCCCTG TCCGAGCACA TCAGTCCTGT GGTGGTGATC 
CCGGCTGAGG TGTCGTCTCC AGACAGTGAG CCCGTCCTGG AGAAGGATGA CCTCATGGAC 
ATGGATGCCT CCCAGCAGAG TTTGTTTGAC AACAAGTTTG ATGACATCTT CGGCAGCTCG 
TTGAGCAGTG ACCCCTTCAA TTTCAACAAT CAAAATGGCG TGAACAAGGA TGAGAAGGAC 
CACTTGATTG AACGCCTCTA CAGAGAGATC AGTGGACTGA CAGGACAGCT GGACAACGTG 
AAGATTGAGA GCCAGCGGGC CATGCTGCAG CTGAAGGGTC GCGTGAGTGA GCTGGAAGCA 
GAGCTGGCCG AGCAGCAGCA CCTGGGACGG CAGGCTACGG ATGACTGCGA GTTCCTGCGC 
ACGGAGCTGG ATGAACTGAA GAGGCAGCGA GAGGACACTG AGAAGGCGCA GCGCAGCCTG 
ACTGAGATAG AAAAAAAGGC CCAGGCCAAT GAACAGCGGT ATAGCAAGTT AAAAGAGAAG 
TACAGTGAAC TGGTGCAGAA CCATGCTGAT CTGCTGCGGA AGAATGCAGA GGTGACCAAG 
CAGGTGTCTG TGGCCCGGCA AGCCCAGGTG GATTTGGAAA GAGAGAAAAA AGAACTAGCG 
GATTCCTTTG CACGTGTAAG CGAGCAGACC CAGCGGAAGA CTCAAGAGCA ACAGGATGTT 
CTAGAGACCC TGAAGCACGA ACTGGCCACC AGCAGGCAAG AGCTGCAGGT CCTCCATGGC 
AACCTGGAAA CCTCTGCCCA GTCTGAGGCG AAATGGCTAA CGCAGATCGC GGAGCTGGAG 
AAGGAACAAG GCAGTTTGGC GACAGCCGCC GCTCAAAGAG AGGAAGAGTT CTCAGCCCTC 
CGAGACCAAC TGGAAAGCAC CCAGCTCAAA CTGGCTGGTG CCCAGGATTC CATGTGTCAG 
CAGATAAAGG ACCAGAGGAG AATCCTCTTG GCAGGGGCCA GGAAGGCTGC GGAGCTCGAG 
ATACAGGAGG CCCTGGGCCA GCTTGAAGAG CCTGCCCTCC TCATCAGCTG TGCAGGAGCC 
ACAGATCATC TTCTCTCCAA AGTCAACTCC GTTTCCAGCT GCCTCGAGCA ACTGGAAAAG 
AGCCGCAGCC AGTATCTGGC CTGCCCAGAA GAGATCAGTG AGCTTCTGCA CTCAATAACC 
CTGCTTGGCC ACTTGACCAG TGACACCATC ATTCAGGGGA CTGCCACCAG CCTCCGGGCC 
CCACCAGAGT CAGCCGACTC TCTGACGGAG GCCTGTAGGC AGTATGGCAG AGAAACTCTG 
GCCTATCTGT CCTCCCTAGA GGAAGAGGGG GCCATGGAAA AAGCTGACAC CACAGCCATA 
GCGAACTGCC TCGGCAAGAT CAAGACCATT GGCGAGGAAC TGCTGCCCAG GGGCTTGGAC 
ATCAAGCAGG AAGAGCTGGG TGACCTGGTG GACAAGGAGA TGGCAGCCAC TTCAGCTGCC 
ATTGAAGCTG CCACCACCCG GATAGAGGAA ATTCTCAGTA AGTCCCGAGC AGGAGACACG 
GGAGTCAAGT TGGAGGTGAA CGAGAGGATC CTTGGCTCCT GTACCAGCCT GATGCAAGCT 
ATCAAGGTGC TCGTTGTGGC CTCCAAGGAC CTCCAGAAGG AGATAGTGGA AAGCGGCAGG 
GGTACCGCAT CCCCTAAAGA ATTTTACGCC AAGAACTCTC GGTGGACAGA AGGGCTCATA 
TCTGCCTCCA AAGCTGTTGG TTGGGGAGCT ACCATCATGG TGGATGCTGC TGACCTTGTG 
GTCCAAGGCA AAGGGAAGTT CGAGGAGCTG ATGGTGTGCT CGCATGAGAT TGCCGCTAGC 
ACAGCCCAGC TTGTGGCTGC ATCCAAGGTG AAAGCGAACA AGGGCAGCCT CAATCTGACC 
CAGCTGCAGC AGGCCTCTCG GGGAGTGAAC CAGGCCACGG CCGCTGTGGT GGCCTCAACC 
ATTTCCGGCA AATCTCAAAT TGAGGAAACA GACAGTATGG ACTTCTCAAG CATGACACTG 
ACCCAGATCA AGCGCCAGGA AATGGATTCC CAGGTTAGGG TGCTGGAGCT AGAAAACGAC 
CTGCAGAAGG AGCGCCAGAA ACTAGGAGAG CTTCGGAAAA AACACTACGA GCTGGCTGGT 
GTTGCTGAGG GCTGGGAAGA AGGGACAGAA GCATCACCTT CTGCTGTTCA AGAAGCGGTG 
CCGGACAAAG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249162.1
CDS	179..3193
Translation	MDVNKMTVSVNKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCSIYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDIFGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQHLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSVARQAQVDLEREKKELADSFARVSEQTQRKTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLTQIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEALGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGTATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQEELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQLVAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVLELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE

Target ORF information:

RefSeq Version	XM_006249100.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus huntingtin interacting protein 1 (Hip1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249100.3

ORF Insert Sequence:

ATGGATGTGA ACAAGATGAC GGTCAGCGTC AATAAGGCCA TTAATACGCA GGAAGTGGCC 
GTGAAGGAAA AACATGCCAG GACGTGTATT CTGGGCACCC ACCATGAGAA AGGGGCGCAG 
ACCTTCTGGT CTGTGGTCAA CCGACTGCCT CTCTCCAGCA ACGCCATGCT GTGCTGGAAG 
TTCTGCCACG TGTTCCACAA ACTCCTTCGA GACGGACATC CGAACGTGCT GAAAGACTCT 
CTCAGATACA AAAATGAGTT AAGCGACATG AGCAGGATGT GGGGCCACCT GAGTGAGGGG 
TATGGACAGC TCTGCAGCAT CTACCTCAAA CTGCTGAGAA CAAGGATGGA GTACCACACC 
AAAAATCCCA GGTTCCCAGG TAACCTCCAG ATGAGCGACC GGCAGCTGGA TGAAGCTGGG 
GAAAGTGATG TTAACAACTT TTTCCAGCTC ACAGTGGAGA TGTTTGATTA CCTGGAGTGC 
GAACTGAACC TCTTTCAAAC TGTGTTCAAC TCCTTGGACA TGTCCCGCTC AGTGTCCGTG 
ACCACAGCTG GCCAGTGCCG CCTCGCCCCG CTCATCCAGG TCATCCTGGA CTGCAGTCAC 
CTCTATGACT ACACTGTCAA GCTGCTCTTC AAGCTCCACT CCTGTCTTCC AGCTGACACC 
CTGCAGGGCC ACCGAGACCG CTTCATGGAG CAGTTCACAA AGTTGAAAGA TCTGTTTCAG 
CGCTCCAGCA ACCTTCAGTA CTTCAAGCGG CTCATTCAGA TCCCCCAGTT GCCTGAGAAT 
CCACCCAACT TCCTACGAGC CTCGGCCCTG TCCGAGCACA TCAGTCCTGT GGTGGTGATC 
CCGGCTGAGG TGTCGTCTCC AGACAGTGAG CCCGTCCTGG AGAAGGATGA CCTCATGGAC 
ATGGATGCCT CCCAGCAGAG TTTGTTTGAC AACAAGTTTG ATGACATCTT CGGCAGCTCG 
TTGAGCAGTG ACCCCTTCAA TTTCAACAAT CAAAATGGCG TGAACAAGGA TGAGAAGGAC 
CACTTGATTG AACGCCTCTA CAGAGAGATC AGTGGACTGA CAGGACAGCT GGACAACGTG 
AAGATTGAGA GCCAGCGGGC CATGCTGCAG CTGAAGGGTC GCGTGAGTGA GCTGGAAGCA 
GAGCTGGCCG AGCAGCAGCA CCTGGGACGG CAGGCTACGG ATGACTGCGA GTTCCTGCGC 
ACGGAGCTGG ATGAACTGAA GAGGCAGCGA GAGGACACTG AGAAGGCGCA GCGCAGCCTG 
ACTGAGATAG AAAAAAAGGC CCAGGCCAAT GAACAGCGGT ATAGCAAGTT AAAAGAGAAG 
TACAGTGAAC TGGTGCAGAA CCATGCTGAT CTGCTGCGGA AGAATGCAGA GGTGACCAAG 
CAGGTGTCTG TGGCCCGGCA AGCCCAGGTG GATTTGGAAA GAGAGAAAAA AGAACTAGCG 
GATTCCTTTG CACGTGTAAG CGAGCAGACC CAGCGGAAGA CTCAAGAGCA ACAGGATGTT 
CTAGAGACCC TGAAGCACGA ACTGGCCACC AGCAGGCAAG AGCTGCAGGT CCTCCATGGC 
AACCTGGAAA CCTCTGCCCA GTCTGAGGCG AAATGGCTAA CGCAGATCGC GGAGCTGGAG 
AAGGAACAAG GCAGTTTGGC GACAGCCGCC GCTCAAAGAG AGGAAGAGTT CTCAGCCCTC 
CGAGACCAAC TGGAAAGCAC CCAGCTCAAA CTGGCTGGTG CCCAGGATTC CATGTGTCAG 
CAGATAAAGG ACCAGAGGAG AATCCTCTTG GCAGGGGCCA GGAAGGCTGC GGAGCTCGAG 
ATACAGGAGG CCCTGGGCCA GCTTGAAGAG CCTGCCCTCC TCATCAGCTG TGCAGGAGCC 
ACAGATCATC TTCTCTCCAA AGTCAACTCC GTTTCCAGCT GCCTCGAGCA ACTGGAAAAG 
AGCCGCAGCC AGTATCTGGC CTGCCCAGAA GAGATCAGTG AGCTTCTGCA CTCAATAACC 
CTGCTTGGCC ACTTGACCAG TGACACCATC ATTCAGGGGA CTGCCACCAG CCTCCGGGCC 
CCACCAGAGT CAGCCGACTC TCTGACGGAG GCCTGTAGGC AGTATGGCAG AGAAACTCTG 
GCCTATCTGT CCTCCCTAGA GGAAGAGGGG GCCATGGAAA AAGCTGACAC CACAGCCATA 
GCGAACTGCC TCGGCAAGAT CAAGACCATT GGCGAGGAAC TGCTGCCCAG GGGCTTGGAC 
ATCAAGCAGG AAGAGCTGGG TGACCTGGTG GACAAGGAGA TGGCAGCCAC TTCAGCTGCC 
ATTGAAGCTG CCACCACCCG GATAGAGGAA ATTCTCAGTA AGTCCCGAGC AGGAGACACG 
GGAGTCAAGT TGGAGGTGAA CGAGAGGATC CTTGGCTCCT GTACCAGCCT GATGCAAGCT 
ATCAAGGTGC TCGTTGTGGC CTCCAAGGAC CTCCAGAAGG AGATAGTGGA AAGCGGCAGG 
GGTACCGCAT CCCCTAAAGA ATTTTACGCC AAGAACTCTC GGTGGACAGA AGGGCTCATA 
TCTGCCTCCA AAGCTGTTGG TTGGGGAGCT ACCATCATGG TGGATGCTGC TGACCTTGTG 
GTCCAAGGCA AAGGGAAGTT CGAGGAGCTG ATGGTGTGCT CGCATGAGAT TGCCGCTAGC 
ACAGCCCAGC TTGTGGCTGC ATCCAAGGTG AAAGCGAACA AGGGCAGCCT CAATCTGACC 
CAGCTGCAGC AGGCCTCTCG GGGAGTGAAC CAGGCCACGG CCGCTGTGGT GGCCTCAACC 
ATTTCCGGCA AATCTCAAAT TGAGGAAACA GACAGTATGG ACTTCTCAAG CATGACACTG 
ACCCAGATCA AGCGCCAGGA AATGGATTCC CAGGTTAGGG TGCTGGAGCT AGAAAACGAC 
CTGCAGAAGG AGCGCCAGAA ACTAGGAGAG CTTCGGAAAA AACACTACGA GCTGGCTGGT 
GTTGCTGAGG GCTGGGAAGA AGGGACAGAA GCATCACCTT CTGCTGTTCA AGAAGCGGTG 
CCGGACAAAG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sla2p/HIP1/HIP1R family: similar structure, similar function in endocytosis?
Biochemical Society transactions38(Pt 1)187-91(2010 Feb)
Gottfried I,Ehrlich M,Ashery U

Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain.
The Journal of biological chemistry283(47)32870-9(2008 Nov)
Wilbur JD,Chen CY,Manalo V,Hwang PK,Fletterick RJ,Brodsky FM

Partially overlapping distribution of epsin1 and HIP1 at the synapse: analysis by immunoelectron microscopy.
The Journal of comparative neurology494(2)368-79(2006 Jan)
Yao PJ,Bushlin I,Petralia RS

Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo.
The Journal of biological chemistry280(7)6109-17(2005 Feb)
Chen CY,Brodsky FM

HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains.
The Journal of biological chemistry279(14)14294-306(2004 Apr)
Hyun TS,Rao DS,Saint-Dic D,Michael LE,Kumar PD,Bradley SV,Mizukami IF,Oravecz-Wilson KI,Ross TS

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Hip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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