Ap1g1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap1g1
Entrez Gene ID	171494
Full Name	adaptor-related protein complex 1, gamma 1 subunit
General protein information	Preferred Names adaptor-related protein complex 1, gamma 1 subunit Names AP-1 complex subunit gamma-1 adaptor protein complex AP-1, gamma 1 subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	may play a role in directing proteins to clathrin-coated vesicles [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_134460.1	NP_604455.1	AP-1 complex subunit gamma-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP1G1	NP_001178365.1
Homo sapiens (human)	AP1G1	NP_001025178.1
Canis lupus familiaris (dog)	AP1G1	XP_003434755.1
Mus musculus (house mouse)	Ap1g1	NP_033807.2
Rattus norvegicus (Norway rat)	Ap1g1	NP_604455.1
Gallus gallus (chicken)	AP1G1	NP_001006132.1
Drosophila melanogaster (fruit fly)	AP-1gamma	NP_788890.2
Caenorhabditis elegans (roundworm)	apg-1	NP_740937.1
Neurospora crassa	NCU04121	XP_961277.2
Xenopus tropicalis (tropical clawed frog)	ap1g1	XP_002933729.2
Pan troglodytes (chimpanzee)	AP1G1	XP_001171470.1
Schizosaccharomyces pombe (fission yeast)	apl4	NP_588559.1
Oryza sativa (rice)	Os06g0167100	NP_001056918.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008251	XP_317218.3
Magnaporthe oryzae (rice blast fungus)	MGG_11801	XP_003709615.1
Arabidopsis thaliana (thale cress)	GAMMA-ADAPTIN 1	NP_173802.1
Arabidopsis thaliana (thale cress)	AT1G60070	NP_001185270.1
Danio rerio (zebrafish)	ap1g1	NP_955976.1

Gene Ontology
Bibliography

Related articles in PubMed

LAMP1/CD107a is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity.
Krzewski K, Gil-Krzewska A, Nguyen V, Peruzzi G, Coligan JE
Blood121(23)4672-83(2013 Jun)

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
Bultema JJ, Ambrosio AL, Burek CL, Di Pietro SM
The Journal of biological chemistry287(23)19550-63(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis.
Delevoye C, Hurbain I, Tenza D, Sibarita JB, Uzan-Gafsou S, Ohno H, Geerts WJ, Verkleij AJ, Salamero J, Marks MS, Raposo G
The Journal of cell biology187(2)247-64(2009 Oct)

Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.
Lee I, Drake MT, Traub LM, Kornfeld S
Archives of biochemistry and biophysics479(1)63-8(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cytosolic AP-1 is recruited onto the trans-Golgi network via binding to Arf-1.GTP, cargo-sorting signals and phophoinositides, where it orchestrates the assembly of clathrin-coated vesicular carriers that transport cargo molecules to endosomes.
Title: Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.

Synaptophysin and gamma-adaptin occurred in the perinuclear region of hippocampal neurons, suggesting that synaptophysin serves as a docking site for AP-1 during clathrin-dependent vesicle budding and/or kinesin-based transport reactions.
Title: Interaction of synaptophysin with the AP-1 adaptor protein gamma-adaptin.

The following Ap1g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap1g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01438	NM_134460.1 Latest version!	Rattus norvegicus adaptor related protein complex 1 subunit gamma 1 (Ap1g1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap1g1 ( NM_134460.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_604455.1 Rattus norvegicus adaptor related protein complex 1 subunit gamma 1 (Ap1g1), mRNA.

Price & Availability↑

CloneID	ORa01438
Clone ID Related Accession (Same CDS sequence)	NM_134460.1
Accession Version	NM_134460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-08
Organism	Rattus norvegicus(Norway rat)
Product	AP-1 complex subunit gamma-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166845.1. On or before Jun 8, 2008 this sequence version replaced XM_341686.3, XM_001072391.1, XM_001075400.1, XM_001075367.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906348, SAMN01906349 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCGA TCCGGTCATC ATTCAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAGTTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTGTTATTAG ATGAAAGACA AGACGTACAT 
CTTCTCATGA CCAATTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGA 
CTAGCACTTT GTACCCTTGG CTGCATGGGC TCCTCAGAGA TGTGCAGAGA TCTTGCAGGA 
GAGGTAGAGA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGCTTGTGCC CCAATTAGTT 
CGTATTCTAA AGAACCTCAT CATGTCCGGA TATTCCCCAG AACATGATGT TTCTGGTATC 
AGTGACCCCT TTTTGCAGGT ACGAATTTTG CGGTTATTAA GAATTTTAGG ACGGAATGAT 
GATGATTCAA GCGAAGCTAT GAATGATATA TTAGCACAGG TCGCCACTAA TACAGAGACC 
AGTAAGAATG TAGGGAATGC TATTCTGTAT GAAACGGTTT TGACTATCAT GGATATTAAA 
TCAGAGAGTG GATTGCGTGT TCTAGCCATA AATATTCTTG GTCGATTCTT ATTGAACAAT 
GACAAAAATA TTAGATATGT TGCTCTGACA TCTCTGTTGA AGACTGTGCA GACAGATCAT 
AATGCTGTAC AGAGGCATAG AAGCACAATT GTGGACTGTC TAAAAGATCT GGATGTCTCC 
ATAAAAAGAC GTGCAATGGA ATTGAGTTTT GCCCTGGTGA ATGGGAATAA TATCCGAGGC 
ATGATGAAGG AGTTACTTTA TTTTCTGGAT TCTTGTGAAC CAGAATTTAA AGCGGATTGT 
GCATCTGGAG TCTTCCTTGC TGCAGAAAAG TACGCACCTT CCAAACGGTG GCATATAGAT 
ACAATTATGC GTGTCTTGAC AACGGCAGGA AGTTATGTTC GTGATGATGC AGTTCCCAAC 
TTGATTCAGT TAATAACCAA CAGTGTGGAG ATGCACGCTT ACACCGTCCA GCGCCTGTAC 
AAGGCAATTC TCGGTGATTA TTCTCAACAA CCCTTGGTAC AGGTGGCCGC ATGGTGTATA 
GGTGAATATG GTGATCTCCT TGTGTCTGGC CAGTGTGAAG AGGAAGAGCC TATTCAGGTG 
ACAGAAGATG AAGTGCTGGA TATTTTAGAA AGTGTCCTGA TCTCTAATAT GTCTACCTCT 
GTGACAAGAG GTTATGCTCT CACTGCCATT ATGAAGCTGT CTACTCGATT CACCTGTACT 
GTAAACCGAA TTAAGAAAGT AGTTTCCATC TATGGAAGCA GCATCGACGT AGAGCTCCAA 
CAGAGGGCAG TAGAATACAA TGCACTTTTT AAGAAGTATG ACCACATGAG GTCTGCCCTA 
CTTGAAAGAA TGCCTGTCAT GGAAAAAGTG ACCACAAATG GCCCTTCTGA GATTGTGCAG 
ACAAATGGAG AGACAGAACC AGCTCCACTA GAGACTAAAC CACCACCCTC TGGGCCACAA 
CCCACGAGCC AGGCCAATGA TCTATTGGAC CTGTTGGGAG GAAATGACAT AACACCTGTT 
ATTCCAACTG CACCTACAAG CAAACCAGCG TCTGCTGGTG GGGAGCTTCT TGACTTGCTG 
GGAGACATCA CCCTAACAGG TGCTCCAGCT GCTGCTCCCA CCCCTGCCTC AGTGCCACAG 
ATAACCCAGC CCCCATTCTT GTTGGATGGG CTTTCGTCAC AGCCTCTCTT CAATGACATC 
GCTCCAGGCA TCCCCTCCAT CACAGCGTAC AGTAAGAACG GCTTGAAGAT AGAATTCACC 
TTTGAGCGGT CAAACACCAA CCCCAGTGTA ACAGTGATAA CGATACAGGC CTCTAACAGC 
ACAGAGCTAG ACATGACGGA CTTTGTTTTC CAGGCTGCAG TACCAAAGAC ATTCCAGCTG 
CAGCTTCTGT CTCCTAGCAG CAGCGTCGTC CCAGCCTTTA ATACAGGGAC CATCACACAA 
GTCATTAAAG TTCTGAACCC ACAGAAGCAA CAGCTGCGAA TGCGGATCAA GCTCACATAC 
AATCACAAGG GCTCAGCAAT GCAGGATCTC GCAGAAGTGA ACAATTTCCC CCCTCAGTCC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_604455.1
CDS	18..2486
Translation	MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGVFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQITQPPFLLDGLSSQPLFNDIAPGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ

Target ORF information:

RefSeq Version	NM_134460.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor related protein complex 1 subunit gamma 1 (Ap1g1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134460.1

ORF Insert Sequence:

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCGA TCCGGTCATC ATTCAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAGTTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTGTTATTAG ATGAAAGACA AGACGTACAT 
CTTCTCATGA CCAATTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGA 
CTAGCACTTT GTACCCTTGG CTGCATGGGC TCCTCAGAGA TGTGCAGAGA TCTTGCAGGA 
GAGGTAGAGA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGCTTGTGCC CCAATTAGTT 
CGTATTCTAA AGAACCTCAT CATGTCCGGA TATTCCCCAG AACATGATGT TTCTGGTATC 
AGTGACCCCT TTTTGCAGGT ACGAATTTTG CGGTTATTAA GAATTTTAGG ACGGAATGAT 
GATGATTCAA GCGAAGCTAT GAATGATATA TTAGCACAGG TCGCCACTAA TACAGAGACC 
AGTAAGAATG TAGGGAATGC TATTCTGTAT GAAACGGTTT TGACTATCAT GGATATTAAA 
TCAGAGAGTG GATTGCGTGT TCTAGCCATA AATATTCTTG GTCGATTCTT ATTGAACAAT 
GACAAAAATA TTAGATATGT TGCTCTGACA TCTCTGTTGA AGACTGTGCA GACAGATCAT 
AATGCTGTAC AGAGGCATAG AAGCACAATT GTGGACTGTC TAAAAGATCT GGATGTCTCC 
ATAAAAAGAC GTGCAATGGA ATTGAGTTTT GCCCTGGTGA ATGGGAATAA TATCCGAGGC 
ATGATGAAGG AGTTACTTTA TTTTCTGGAT TCTTGTGAAC CAGAATTTAA AGCGGATTGT 
GCATCTGGAG TCTTCCTTGC TGCAGAAAAG TACGCACCTT CCAAACGGTG GCATATAGAT 
ACAATTATGC GTGTCTTGAC AACGGCAGGA AGTTATGTTC GTGATGATGC AGTTCCCAAC 
TTGATTCAGT TAATAACCAA CAGTGTGGAG ATGCACGCTT ACACCGTCCA GCGCCTGTAC 
AAGGCAATTC TCGGTGATTA TTCTCAACAA CCCTTGGTAC AGGTGGCCGC ATGGTGTATA 
GGTGAATATG GTGATCTCCT TGTGTCTGGC CAGTGTGAAG AGGAAGAGCC TATTCAGGTG 
ACAGAAGATG AAGTGCTGGA TATTTTAGAA AGTGTCCTGA TCTCTAATAT GTCTACCTCT 
GTGACAAGAG GTTATGCTCT CACTGCCATT ATGAAGCTGT CTACTCGATT CACCTGTACT 
GTAAACCGAA TTAAGAAAGT AGTTTCCATC TATGGAAGCA GCATCGACGT AGAGCTCCAA 
CAGAGGGCAG TAGAATACAA TGCACTTTTT AAGAAGTATG ACCACATGAG GTCTGCCCTA 
CTTGAAAGAA TGCCTGTCAT GGAAAAAGTG ACCACAAATG GCCCTTCTGA GATTGTGCAG 
ACAAATGGAG AGACAGAACC AGCTCCACTA GAGACTAAAC CACCACCCTC TGGGCCACAA 
CCCACGAGCC AGGCCAATGA TCTATTGGAC CTGTTGGGAG GAAATGACAT AACACCTGTT 
ATTCCAACTG CACCTACAAG CAAACCAGCG TCTGCTGGTG GGGAGCTTCT TGACTTGCTG 
GGAGACATCA CCCTAACAGG TGCTCCAGCT GCTGCTCCCA CCCCTGCCTC AGTGCCACAG 
ATAACCCAGC CCCCATTCTT GTTGGATGGG CTTTCGTCAC AGCCTCTCTT CAATGACATC 
GCTCCAGGCA TCCCCTCCAT CACAGCGTAC AGTAAGAACG GCTTGAAGAT AGAATTCACC 
TTTGAGCGGT CAAACACCAA CCCCAGTGTA ACAGTGATAA CGATACAGGC CTCTAACAGC 
ACAGAGCTAG ACATGACGGA CTTTGTTTTC CAGGCTGCAG TACCAAAGAC ATTCCAGCTG 
CAGCTTCTGT CTCCTAGCAG CAGCGTCGTC CCAGCCTTTA ATACAGGGAC CATCACACAA 
GTCATTAAAG TTCTGAACCC ACAGAAGCAA CAGCTGCGAA TGCGGATCAA GCTCACATAC 
AATCACAAGG GCTCAGCAAT GCAGGATCTC GCAGAAGTGA ACAATTTCCC CCCTCAGTCC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LAMP1/CD107a is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity.
Blood121(23)4672-83(2013 Jun)
Krzewski K,Gil-Krzewska A,Nguyen V,Peruzzi G,Coligan JE

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
The Journal of biological chemistry287(23)19550-63(2012 Jun)
Bultema JJ,Ambrosio AL,Burek CL,Di Pietro SM

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis.
The Journal of cell biology187(2)247-64(2009 Oct)
Delevoye C,Hurbain I,Tenza D,Sibarita JB,Uzan-Gafsou S,Ohno H,Geerts WJ,Verkleij AJ,Salamero J,Marks MS,Raposo G

Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.
Archives of biochemistry and biophysics479(1)63-8(2008 Nov)
Lee I,Drake MT,Traub LM,Kornfeld S