Map3k2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k2
Entrez Gene ID	171492
Full Name	mitogen activated protein kinase kinase kinase 2
Synonyms	Mekk2
General protein information	Preferred Names mitogen activated protein kinase kinase kinase 2 Names mitogen-activated protein kinase kinase kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p12
Summary	activates MAPK/ERK kinase 1; may play a role in cell proliferation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138503.1	NP_612512.1	mitogen-activated protein kinase kinase kinase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP3K2	XP_001137344.1
Rattus norvegicus (Norway rat)	Map3k2	NP_612512.1
Gallus gallus (chicken)	MAP3K2	XP_004942945.1
Canis lupus familiaris (dog)	MAP3K2	XP_005631936.1
Mus musculus (house mouse)	Map3k2	NP_036076.2
Homo sapiens (human)	MAP3K2	NP_006600.3
Xenopus tropicalis (tropical clawed frog)	map3k2	XP_002942055.2
	MAP3K2	XP_002799467.1
Bos taurus (cattle)	MAP3K2	XP_002685278.2
Danio rerio (zebrafish)	map3k2	XP_686613.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the Bcl-2 protein BAD promotes prostate cancer growth.
Smith AJ, Karpova Y, D'Agostino R Jr, Willingham M, Kulik G
PloS one4(7)e6224(2009 Jul)

Negative regulation of MEKK1/2 signaling by serine-threonine kinase 38 (STK38).
Enomoto A, Kido N, Ito M, Morita A, Matsumoto Y, Takamatsu N, Hosoi Y, Miyagawa K
Oncogene27(13)1930-8(2008 Mar)

The c-jun kinase/stress-activated pathway: regulation, function and role in human disease.
Johnson GL, Nakamura K
Biochimica et biophysica acta1773(8)1341-8(2007 Aug)

Brain-derived neurotrophic factor activates ERK5 in cortical neurons via a Rap1-MEKK2 signaling cascade.
Wang Y, Su B, Xia Z
The Journal of biological chemistry281(47)35965-74(2006 Nov)

Regulation of cellular functions by the ERK5 signalling pathway.
Wang X, Tournier C
Cellular signalling18(6)753-60(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rap1 and MEKK2 are critical upstream signaling molecules mediating BDNF stimulation of ERK5 in central nervous system neurons
Title: Brain-derived neurotrophic factor activates ERK5 in cortical neurons via a Rap1-MEKK2 signaling cascade.

Unlike ERK5 and MEK5, their upstream activator MEKK2 is localized mainly in the cytosol of resting cells, and translocates into the nucleus upon EGF stimulation
Title: MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation.

The following Map3k2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01436	NM_138503.1 Latest version!	Rattus norvegicus mitogen activated protein kinase kinase kinase 2 (Map3k2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k2 ( NM_138503.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612512.1 Rattus norvegicus mitogen activated protein kinase kinase kinase 2 (Map3k2), mRNA.

Price & Availability↑

CloneID	ORa01436
Clone ID Related Accession (Same CDS sequence)	NM_138503.1
Accession Version	NM_138503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC112062.5 and AC126902.5. On or before Dec 19, 2009 this sequence version replaced XM_001059840.1, XM_001067270.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATC AGCAAGCTTT GAATTCAATC ATGCAAGATT TGGCTGTCCT TCATAAGGCC 
AGTCGGCCAG CATTATCTTT ACAAGAAACC AGAAAAGCAA AACCCTCTTC ACCAAAAAAA 
CAGAATGATG TCCGAGTCAA ATTTGAACAT AGAGGAGAAA AAAGAATCTT GCAGGTTACT 
AGACCAGTTA AACTAGAAGA TCTGAGGTCT AAGTCTAAAA TTGCCTTTGG ACAGTCTATG 
GATCTACACT ATACCAACAA CGAGTTGGTA ATTCCGTTAA CTACCCAAGA TGACTTGGAC 
AAAGCTGTGG AACTGCTGGA TCGCAGTATC CACATGAAGA GTCTCAAGAT ATTACTTGTT 
CTAAATGGGA GTACACAGGC TACTAATTTA GAGCCGTCAC CATCACCAGA CGATCTGAAT 
AATACACCAC TCGGTGCGGA GAGGAAAAAG CGGCTATCTG TAGTAGGTCC CCCTAATAGG 
GATAGAAGTT CTCCACCCCC AGGATACATT CCAGATGAAC TGCACCAGAT TGCCCGGAAC 
GGGTCTTTCA CCAGCATCAA CAGTGAAGGA GAGTTCATCC CAGAGAGCAT GGACCAAATG 
CTGGATCCAC TGTCTTTAAG CAGCCCTGAA AACTCTGGCT CAGGAAGCTG CCCGTCACTG 
GATAGTCCTT TGGATGGAGA AAGCTACCCA AAATCACGGA TGCCTAGGGC ACAGAGCTAC 
CCAGATAATC ATCAGGAGTT TTCAGACTAT GATAACCCCA TTTTTGAGAA ATTTGGAAAA 
GGAGGAACAT ATCCAAGAAG GTATCATGTT TCATACCATC ATCAAGAGTA TAATGATGGT 
CGGAAGACTT TTCCAAGAGC CAGAAGGACC CAGGGCACCA GTTTCCGGTC TCCTGTGAGT 
TTCAGTCCTA CTGATCACTC CTTAAGCACT AGTAGTGGGA GCAGCATCTT TACCCCAGAG 
TATGAAGACA GTCGGATAAG AAGACGGGGG AGTGACATAG ACAATCCTAC TTTGACTGTC 
ACAGACATCA GCCCACCCAG CCGTTCACCT CGAGCTCCAA CCAACTGGAG ATTGGGCAAA 
CTGCTTGGCC AAGGAGCTTT TGGAAGGGTC TACCTCTGTT ATGATGTTGA TACCGGACGA 
GAACTGGCTG TTAAACAAGT TCAGTTTAAC CCTGACAGCC CCGAGACCAG CAAGGAAGTA 
AATGCACTTG AGTGTGAAAT TCAGTTGTTG AAAAACTTGT TGCATGAGCG AATTGTTCAG 
TATTATGGCT GTCTGAGGGA TCCTCAGGAG AAAACACTTT CCATCTTTAT GGAGTACATG 
CCAGGGGGCT CAATTAAGGA CCAACTCAAA GCATATGGAG CCCTTACTGA GAATGTGACG 
AGGAAATACA CCCGTCAGAT TCTGGAGGGG GTCCATTACC TGCATAGTAA TATGATTGTC 
CATAGAGACA TCAAAGGAGC GAATATCTTA AGGGATTCCA CGGGCAATAT CAAGCTAGGA 
GACTTTGGAG CTAGCAAACG ACTTCAGACC ATCTGTCTCT CAGGCACAGG GATGAAGTCT 
GTCACAGGCA CGCCATATTG GATGAGTCCT GAGGTCATTA GTGGAGAAGG CTATGGCAGA 
AAAGCAGATA TCTGGAGCGT AGCATGTACG GTGGTGGAGA TGCTAACTGA AAAGCCACCT 
TGGGCTGAAT TTGAAGCAAT GGCTGCCATC TTTAAGATCG CCACTCAGCC AACAAACCCA 
AAGCTGCCAC CTCATGTCTC AGACTATACT CGGGACTTTC TCAAACGGAT TTTTGTAGAG 
GCCAAACTCC GACCTTCAGC CGAGGAGCTC CTGCGGCACC TGTTCGTGCA TTGCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612512.1
CDS	509..2368
Translation	MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKPSSPKKQNDVRVKFEHRGEKRILQVTRPVKLEDLRSKSKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVLNGSTQATNLEPSPSPDDLNNTPLGAERKKRLSVVGPPNRDRSSPPPGYIPDELHQIARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSFRSPVSFSPTDHSLSTSSGSSIFTPEYEDSRIRRRGSDIDNPTLTVTDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFNPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSAEELLRHLFVHCH

Target ORF information:

RefSeq Version	NM_138503.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen activated protein kinase kinase kinase 2 (Map3k2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138503.1

ORF Insert Sequence:

ATGGATGATC AGCAAGCTTT GAATTCAATC ATGCAAGATT TGGCTGTCCT TCATAAGGCC 
AGTCGGCCAG CATTATCTTT ACAAGAAACC AGAAAAGCAA AACCCTCTTC ACCAAAAAAA 
CAGAATGATG TCCGAGTCAA ATTTGAACAT AGAGGAGAAA AAAGAATCTT GCAGGTTACT 
AGACCAGTTA AACTAGAAGA TCTGAGGTCT AAGTCTAAAA TTGCCTTTGG ACAGTCTATG 
GATCTACACT ATACCAACAA CGAGTTGGTA ATTCCGTTAA CTACCCAAGA TGACTTGGAC 
AAAGCTGTGG AACTGCTGGA TCGCAGTATC CACATGAAGA GTCTCAAGAT ATTACTTGTT 
CTAAATGGGA GTACACAGGC TACTAATTTA GAGCCGTCAC CATCACCAGA CGATCTGAAT 
AATACACCAC TCGGTGCGGA GAGGAAAAAG CGGCTATCTG TAGTAGGTCC CCCTAATAGG 
GATAGAAGTT CTCCACCCCC AGGATACATT CCAGATGAAC TGCACCAGAT TGCCCGGAAC 
GGGTCTTTCA CCAGCATCAA CAGTGAAGGA GAGTTCATCC CAGAGAGCAT GGACCAAATG 
CTGGATCCAC TGTCTTTAAG CAGCCCTGAA AACTCTGGCT CAGGAAGCTG CCCGTCACTG 
GATAGTCCTT TGGATGGAGA AAGCTACCCA AAATCACGGA TGCCTAGGGC ACAGAGCTAC 
CCAGATAATC ATCAGGAGTT TTCAGACTAT GATAACCCCA TTTTTGAGAA ATTTGGAAAA 
GGAGGAACAT ATCCAAGAAG GTATCATGTT TCATACCATC ATCAAGAGTA TAATGATGGT 
CGGAAGACTT TTCCAAGAGC CAGAAGGACC CAGGGCACCA GTTTCCGGTC TCCTGTGAGT 
TTCAGTCCTA CTGATCACTC CTTAAGCACT AGTAGTGGGA GCAGCATCTT TACCCCAGAG 
TATGAAGACA GTCGGATAAG AAGACGGGGG AGTGACATAG ACAATCCTAC TTTGACTGTC 
ACAGACATCA GCCCACCCAG CCGTTCACCT CGAGCTCCAA CCAACTGGAG ATTGGGCAAA 
CTGCTTGGCC AAGGAGCTTT TGGAAGGGTC TACCTCTGTT ATGATGTTGA TACCGGACGA 
GAACTGGCTG TTAAACAAGT TCAGTTTAAC CCTGACAGCC CCGAGACCAG CAAGGAAGTA 
AATGCACTTG AGTGTGAAAT TCAGTTGTTG AAAAACTTGT TGCATGAGCG AATTGTTCAG 
TATTATGGCT GTCTGAGGGA TCCTCAGGAG AAAACACTTT CCATCTTTAT GGAGTACATG 
CCAGGGGGCT CAATTAAGGA CCAACTCAAA GCATATGGAG CCCTTACTGA GAATGTGACG 
AGGAAATACA CCCGTCAGAT TCTGGAGGGG GTCCATTACC TGCATAGTAA TATGATTGTC 
CATAGAGACA TCAAAGGAGC GAATATCTTA AGGGATTCCA CGGGCAATAT CAAGCTAGGA 
GACTTTGGAG CTAGCAAACG ACTTCAGACC ATCTGTCTCT CAGGCACAGG GATGAAGTCT 
GTCACAGGCA CGCCATATTG GATGAGTCCT GAGGTCATTA GTGGAGAAGG CTATGGCAGA 
AAAGCAGATA TCTGGAGCGT AGCATGTACG GTGGTGGAGA TGCTAACTGA AAAGCCACCT 
TGGGCTGAAT TTGAAGCAAT GGCTGCCATC TTTAAGATCG CCACTCAGCC AACAAACCCA 
AAGCTGCCAC CTCATGTCTC AGACTATACT CGGGACTTTC TCAAACGGAT TTTTGTAGAG 
GCCAAACTCC GACCTTCAGC CGAGGAGCTC CTGCGGCACC TGTTCGTGCA TTGCCACTAG