Ap3m1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap3m1
Entrez Gene ID	171126
Full Name	adaptor-related protein complex 3, mu 1 subunit
Synonyms	P47a
General protein information	Preferred Names adaptor-related protein complex 3, mu 1 subunit Names AP-3 complex subunit mu-1 AP-3 adapter complex mu3A subunit AP-3 adaptor complex mu3A subunit HA1 47 kDa subunit homolog 1 Mu-adaptin 3A adapter-related protein complex 3 mu-1 subunit adapter-related protein complex 3 subunit mu-1 adaptor-related protein complex 3 subunit mu-1 adaptor-related protein complex AP-3, mu 1 subunit clathrin assembly protein assembly protein complex 1 medium chain homolog 1 clathrin assembly protein assembly protein complex 3 mu-1 medium chain clathrin coat assembly protein AP47 homolog 1 clathrin coat-associated protein AP47 homolog 1 clathrin-associated adaptor protein homolog (p47A) golgi adaptor AP-1 47 kDa protein homolog 1 mu3A-adaptin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p16
Summary	may act as an adaptor complex subunit; may play a role in intracellular transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251629.2	XP_006251691.1	AP-3 complex subunit mu-1 isoform X1
NM_133593.2	NP_598277.2	AP-3 complex subunit mu-1
XM_006251630.3	XP_006251692.1	AP-3 complex subunit mu-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP3M1	XP_001147572.1
Mus musculus (house mouse)	Ap3m1	NP_061299.3
Danio rerio (zebrafish)	ap3m1	NP_958449.1
Caenorhabditis elegans (roundworm)	apm-3	NP_508184.2
Bos taurus (cattle)	AP3M1	NP_001069148.1
Gallus gallus (chicken)	AP3M1	NP_001026398.1
	AP3M1	XP_001098637.1
Rattus norvegicus (Norway rat)	Ap3m1	NP_598277.2
Homo sapiens (human)	AP3M1	NP_036227.1
Canis lupus familiaris (dog)	AP3M1	XP_003434525.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for the recognition of tyrosine-based sorting signals by the ?3A subunit of the AP-3 adaptor complex.
Mardones GA, Burgos PV, Lin Y, Kloer DP, Magad?n JG, Hurley JH, Bonifacino JS
The Journal of biological chemistry288(13)9563-71(2013 Mar)

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
Bultema JJ, Ambrosio AL, Burek CL, Di Pietro SM
The Journal of biological chemistry287(23)19550-63(2012 Jun)

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Larimore J, Tornieri K, Ryder PV, Gokhale A, Zlatic SA, Craige B, Lee JD, Talbot K, Pare JF, Smith Y, Faundez V
Molecular biology of the cell22(24)4854-67(2011 Dec)

Direct interaction of Dysbindin with the AP-3 complex via its mu subunit.
Taneichi-Kuroda S, Taya S, Hikita T, Fujino Y, Kaibuchi K
Neurochemistry international54(7)431-8(2009 Jun)

Integral and associated lysosomal membrane proteins.
Schr?der B, Wrocklage C, Pan C, J?ger R, K?sters B, Sch?fer H, Els?sser HP, Mann M, Hasilik A
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Structural basis for the recognition of tyrosine-based sorting signals by the mu3A subunit of the AP-3 adaptor complex
Title: Structural basis for the recognition of tyrosine-based sorting signals by the ?3A subunit of the AP-3 adaptor complex.

The following Ap3m1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap3m1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12817	NM_133593.2 Latest version!	Rattus norvegicus adaptor related protein complex 3 subunit mu 1 (Ap3m1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa12817	XM_006251630.3 Latest version!	Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa12817	XM_006251629.2 Latest version!	Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap3m1 ( NM_133593.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598277.2 Rattus norvegicus adaptor related protein complex 3 subunit mu 1 (Ap3m1), mRNA.

Price & Availability↑

CloneID	ORa12817
Clone ID Related Accession (Same CDS sequence)	XM_006251629.2 , NM_133593.2 , XM_006251630.3
Accession Version	NM_133593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	AP-3 complex subunit mu-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC070925.1. On Jun 20, 2004 this sequence version replaced NM_133593.1. ##Evidence-Data-START## Transcript exon combination :: BC070925.1, L07073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598277.2
CDS	31..1287
Translation	MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHNISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQKLPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYITKAGKFQVRT

Target ORF information:

RefSeq Version	NM_133593.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor related protein complex 3 subunit mu 1 (Ap3m1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133593.2

ORF Insert Sequence:

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3m1 ( XM_006251629.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251691.1 Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12817
Clone ID Related Accession (Same CDS sequence)	XM_006251629.2 , NM_133593.2 , XM_006251630.3
Accession Version	XM_006251629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	AP-3 complex subunit mu-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251691.1
CDS	558..1814
Translation	MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHNISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQKLPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYITKAGKFQVRT

Target ORF information:

RefSeq Version	XM_006251629.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251629.2

ORF Insert Sequence:

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3m1 ( XM_006251630.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251692.1 Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12817
Clone ID Related Accession (Same CDS sequence)	XM_006251629.2 , NM_133593.2 , XM_006251630.3
Accession Version	XM_006251630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	AP-3 complex subunit mu-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251692.1
CDS	79..1335
Translation	MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHNISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQKLPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYITKAGKFQVRT

Target ORF information:

RefSeq Version	XM_006251630.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251630.3

ORF Insert Sequence:

ATGATCCACA GTCTATTTCT CATCAACTGT TCTGGCGACA TATTTCTAGA AAAACACTGG 
AAGAGCGTTG TAAGCCAATC TGTGTGTGAC TATTTCTTTG AAGCTCAGGA GAAAGCTGCT 
GATGTTGAAA ATGTACCACC TGTCATTTCA ACACCTCACC ACTACCTCAT TAGTATCTAC 
CGGGATAAGC TCTTCTTTGT GTCTGTGATA CAGACTGAAG TGCCACCTCT CTTTGTAATT 
GAGTTTCTGC ATCGAGTTGC TGACACTTTT CAGGACTACT TTGGTGAGTG TTCAGAGGCT 
GCAATTAAGG ATAATGTGGT CATAGTGTAT GAGCTCTTGG AAGAAATGTT AGACAATGGA 
TTCCCACTGG CTACCGAATC TAACATTCTG AAAGAACTGA TTAAACCACC AACAATTCTA 
CGTTCTGTCG TCAATTCTAT TACAGGCAGT AGTAATGTTG GGGACACGCT CCCCACTGGG 
CAGCTGTCCA ACATCCCGTG GCGTCGAGCA GGTGTAAAGT ACACCAACAA TGAAGCCTAC 
TTTGATGTAG TCGAAGAGAT AGATGCGATT ATAGATAAAT CAGGATCTAC AGTCTTTGCA 
GAAATTCAAG GTGTCATTGA TGCTTGCATT AAGCTGTCTG GAATGCCTGA TCTCTCTCTC 
TCTTTCATGA ACCCAAGGCT TCTAGATGAT GTCAGCTTCC ACCCATGCAT CCGGTTCAAA 
CGCTGGGAAT CTGAGAGAGT TTTGTCATTC ATTCCTCCCG ATGGAAATTT CCGACTCATA 
TCATACCGCG TCAGCTCACA AAATCTAGTG GCAATCCCAG TGTATGTGAA ACATAATATC 
AGCTTTAAGG AAAACAGCTC TTGTGGTAGA TTTGATATAA CAATTGGACC AAAACAGAAT 
ATGGGAAAAA CGATTGAAGG AATCACAGTG ACTGTTCACA TGCCAAAAGT TGTGCTGAAT 
ATGAACCTGA CACCAACACA AGGCAGCTAT ACATTCGATC CAGTCACCAA GGTACTAGCA 
TGGGATGTGG GGAAAATTAC TCCACAAAAG CTCCCAAGTC TTAAAGGACT GGTAAATTTA 
CAGTCAGGAG CACCCAAGCC AGAAGAGAAC CCAAACCTCA ACATACAGTT CAAGATCCAG 
CAGCTTGCTA TTTCAGGCTT AAAAGTGAAC CGCTTGGACA TGTATGGTGA GAAGTATAAG 
CCATTTAAAG GAGTCAAGTA TATCACAAAG GCCGGAAAGT TCCAAGTGAG GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for the recognition of tyrosine-based sorting signals by the ?3A subunit of the AP-3 adaptor complex.
The Journal of biological chemistry288(13)9563-71(2013 Mar)
Mardones GA,Burgos PV,Lin Y,Kloer DP,Magad?n JG,Hurley JH,Bonifacino JS

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
The Journal of biological chemistry287(23)19550-63(2012 Jun)
Bultema JJ,Ambrosio AL,Burek CL,Di Pietro SM

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Molecular biology of the cell22(24)4854-67(2011 Dec)
Larimore J,Tornieri K,Ryder PV,Gokhale A,Zlatic SA,Craige B,Lee JD,Talbot K,Pare JF,Smith Y,Faundez V

Direct interaction of Dysbindin with the AP-3 complex via its mu subunit.
Neurochemistry international54(7)431-8(2009 Jun)
Taneichi-Kuroda S,Taya S,Hikita T,Fujino Y,Kaibuchi K

Integral and associated lysosomal membrane proteins.
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)
Schr?der B,Wrocklage C,Pan C,J?ger R,K?sters B,Sch?fer H,Els?sser HP,Mann M,Hasilik A

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Ap3m1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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