Chrna2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrna2
Entrez Gene ID	170945
Full Name	cholinergic receptor nicotinic alpha 2 subunit
Synonyms	ACHR
General protein information	Preferred Names cholinergic receptor nicotinic alpha 2 subunit Names neuronal acetylcholine receptor subunit alpha-2 cholinergic receptor, nicotinic, alpha 2 (neuronal) cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) nicotinic acetylcholine receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	agonist-binding subunit of neuronal nicotinic acetylcholine receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133420.1	NP_596911.1	neuronal acetylcholine receptor subunit alpha-2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRNA2	XP_005635946.1
Mus musculus (house mouse)	Chrna2	NP_659052.1
Rattus norvegicus (Norway rat)	Chrna2	NP_596911.1
Pan troglodytes (chimpanzee)	CHRNA2	NP_001029107.1
	CHRNA2	XP_001109335.1
Xenopus tropicalis (tropical clawed frog)	chrna2	XP_002941292.2
Homo sapiens (human)	CHRNA2	NP_000733.2
Gallus gallus (chicken)	CHRNA2	NP_990146.1
Bos taurus (cattle)	CHRNA2	NP_001179639.1
Drosophila melanogaster (fruit fly)	nAcRalpha-96Ab	NP_733001.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002972	XP_311921.3

Gene Ontology
Bibliography

Related articles in PubMed

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Richard AF, Demignon J, Sakakibara I, Pujol J, Favier M, Strochlic L, Le Grand F, Sgarioto N, Guernec A, Schmitt A, Cagnard N, Huang R, Legay C, Guillet-Deniau I, Maire P
Developmental biology359(2)303-20(2011 Nov)

Alpha2 nicotine receptors function as a molecular switch to continuously excite a subset of interneurons in rat hippocampal circuits.
Jia Y, Yamazaki Y, Nakauchi S, Sumikawa K
The European journal of neuroscience29(8)1588-603(2009 Apr)

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque EX, Pereira EF, Alkondon M, Rogers SW
Physiological reviews89(1)73-120(2009 Jan)

Postnatal expression of alpha2 nicotinic acetylcholine receptor subunit mRNA in developing cortex and hippocampus.
Son JH, Winzer-Serhan UH
Journal of chemical neuroanatomy32(2-4)179-90(2006 Dec)

Nicotinic cholinergic receptors in the rat retina: simple and mixed heteromeric subtypes.
Marritt AM, Cox BC, Yasuda RP, McIntosh JM, Xiao Y, Wolfe BB, Kellar KJ
Molecular pharmacology68(6)1656-68(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that nicotine alters the normal operation of hippocampal circuits by gating inhibitory circuits through activating a non-desensitizing alpha2 nAChR subtype on a distinct population of interneurons.
Title: Alpha2 nicotine receptors function as a molecular switch to continuously excite a subset of interneurons in rat hippocampal circuits.

Similar to other nAChR subunits, alpha2 mRNA expression is transiently upregulated during postnatal development and nAChRs containing alpha2 subunits could regulate GABAergic activity during a critical period of network formation.
Title: Postnatal expression of alpha2 nicotinic acetylcholine receptor subunit mRNA in developing cortex and hippocampus.

At least two different affinity sites for acetylcholine. Sensitivity to block of alpha4beta2 channels by dihydro-beta-erythroidine was higher than that for alpha2beta2. Current-voltage for alpha2beta2 more strongly rectified than for alpha4beta2.
Title: Rat nicotinic acetylcholine receptor alpha2beta2 channels: comparison of functional properties with alpha4beta2 channels in Xenopus oocytes.

The following Chrna2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrna2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00744	NM_133420.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic alpha 2 subunit (Chrna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrna2 ( NM_133420.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596911.1 Rattus norvegicus cholinergic receptor nicotinic alpha 2 subunit (Chrna2), mRNA.

Price & Availability↑

CloneID	ORa00744
Clone ID Related Accession (Same CDS sequence)	NM_133420.1
Accession Version	NM_133420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal acetylcholine receptor subunit alpha-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L10077.1. ##Evidence-Data-START## Transcript exon combination :: L10077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTTT CCCATTCTGC TCTCCAGTTC TGGACACATC TTTATCTCTG GTGTCTCCTT 
CTGGTGCCAG CAGTGTTGAC CCAGCAAGGC TCACACACCC ATGCTGAGGA CCGCCTGTTC 
AAACACCTGT TTGGAGGCTA CAATCGCTGG GCACGGCCAG TGCCCAACAC TTCTGATGTG 
GTCATCGTGC GCTTTGGATT ATCCATTGCT CAGCTCATAG ATGTGGATGA GAAGAATCAA 
ATGATGACCA CCAATGTCTG GCTAAAGCAG GAATGGAATG ACTACAAGCT GCGCTGGGAC 
CCGGCTGAGT TTGGCAATGT CACCTCCCTG CGCGTCCCTT CAGAGATGAT CTGGATCCCA 
GACATTGTCC TCTACAACAA TGCAGATGGG GAGTTTGCGG TGACCCACAT GACCAAGGCT 
CACCTCTTCT TCACGGGCAC TGTGCACTGG GTGCCCCCAG CCATCTACAA GAGCTCCTGC 
AGCATCGATG TGACCTTCTT CCCCTTCGAC CAGCAGAACT GCAAGATGAA GTTTGGCTCC 
TGGACATATG ACAAGGCCAA GATCGATCTG GAGCAGATGG AGAGGACAGT GGACCTGAAG 
GACTACTGGG AGAGTGGCGA GTGGGCCATT ATCAATGCCA CCGGAACCTA TAACAGTAAG 
AAGTACGACT GCTGCGCGGA GATCTACCCC GATGTCACCT ACTACTTTGT GATCCGCCGG 
CTGCCGCTGT TCTATACCAT CAACCTCATC ATCCCATGCC TGCTCATCTC CTGCCTCACT 
GTGCTCGTGT TCTACCTGCC TTCCGAGTGT GGAGAGAAGA TCACGCTGTG CATCTCGGTG 
CTGCTATCTC TCACTGTCTT CCTGCTGCTC ATCACGGAGA TCATCCCGTC CACCTCGCTG 
GTCATCCCAC TCATCGGCGA GTACCTGCTC TTCACCATGA TCTTTGTCAC CCTCTCTATC 
GTTATCACAG TCTTCGTGCT CAATGTACAC CACCGCTCCC CCAGCACCCA CAACATGCCC 
AACTGGGTAA GGGTAGCCCT GCTAGGCCGG GTGCCCAGGT GGCTGATGAT GAACCGGCCC 
CTGCCACCTA TGGAGCTCCA TGGCTCCCCG GATCTGAAGC TCAGCCCCTC ATACCATTGG 
CTAGAGACTA ACATGGATGC TGGAGAAAGG GAGGAGACAG AGGAAGAGGA GGAAGAAGAA 
GATGAAAACA TATGTGTGTG TGCAGGCCTT CCAGACTCTT CGATGGGTGT CCTCTATGGC 
CATGGCGGCC TGCATCTGAG AGCCATGGAG CCTGAGACCA AGACTCCATC CCAGGCTAGC 
GAGATTCTGC TGTCACCTCA AATACAGAAA GCACTAGAAG GTGTACACTA CATTGCTGAC 
CGTCTGAGGT CTGAGGATGC TGACTCTTCG GTGAAGGAAG ACTGGAAGTA TGTGGCCATG 
GTGGTAGACC GGATATTCCT CTGGCTGTTC ATTATCGTCT GCTTCCTGGG GACCATCGGA 
CTCTTCCTTC CTCCATTCCT GGCTGGAATG ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596911.1
CDS	305..1840
Translation	MTLSHSALQFWTHLYLWCLLLVPAVLTQQGSHTHAEDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNVTSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAIINATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGRVPRWLMMNRPLPPMELHGSPDLKLSPSYHWLETNMDAGEREETEEEEEEEDENICVCAGLPDSSMGVLYGHGGLHLRAMEPETKTPSQASEILLSPQIQKALEGVHYIADRLRSEDADSSVKEDWKYVAMVVDRIFLWLFIIVCFLGTIGLFLPPFLAGMI

Target ORF information:

RefSeq Version	NM_133420.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic alpha 2 subunit (Chrna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133420.1

ORF Insert Sequence:

ATGACCCTTT CCCATTCTGC TCTCCAGTTC TGGACACATC TTTATCTCTG GTGTCTCCTT 
CTGGTGCCAG CAGTGTTGAC CCAGCAAGGC TCACACACCC ATGCTGAGGA CCGCCTGTTC 
AAACACCTGT TTGGAGGCTA CAATCGCTGG GCACGGCCAG TGCCCAACAC TTCTGATGTG 
GTCATCGTGC GCTTTGGATT ATCCATTGCT CAGCTCATAG ATGTGGATGA GAAGAATCAA 
ATGATGACCA CCAATGTCTG GCTAAAGCAG GAATGGAATG ACTACAAGCT GCGCTGGGAC 
CCGGCTGAGT TTGGCAATGT CACCTCCCTG CGCGTCCCTT CAGAGATGAT CTGGATCCCA 
GACATTGTCC TCTACAACAA TGCAGATGGG GAGTTTGCGG TGACCCACAT GACCAAGGCT 
CACCTCTTCT TCACGGGCAC TGTGCACTGG GTGCCCCCAG CCATCTACAA GAGCTCCTGC 
AGCATCGATG TGACCTTCTT CCCCTTCGAC CAGCAGAACT GCAAGATGAA GTTTGGCTCC 
TGGACATATG ACAAGGCCAA GATCGATCTG GAGCAGATGG AGAGGACAGT GGACCTGAAG 
GACTACTGGG AGAGTGGCGA GTGGGCCATT ATCAATGCCA CCGGAACCTA TAACAGTAAG 
AAGTACGACT GCTGCGCGGA GATCTACCCC GATGTCACCT ACTACTTTGT GATCCGCCGG 
CTGCCGCTGT TCTATACCAT CAACCTCATC ATCCCATGCC TGCTCATCTC CTGCCTCACT 
GTGCTCGTGT TCTACCTGCC TTCCGAGTGT GGAGAGAAGA TCACGCTGTG CATCTCGGTG 
CTGCTATCTC TCACTGTCTT CCTGCTGCTC ATCACGGAGA TCATCCCGTC CACCTCGCTG 
GTCATCCCAC TCATCGGCGA GTACCTGCTC TTCACCATGA TCTTTGTCAC CCTCTCTATC 
GTTATCACAG TCTTCGTGCT CAATGTACAC CACCGCTCCC CCAGCACCCA CAACATGCCC 
AACTGGGTAA GGGTAGCCCT GCTAGGCCGG GTGCCCAGGT GGCTGATGAT GAACCGGCCC 
CTGCCACCTA TGGAGCTCCA TGGCTCCCCG GATCTGAAGC TCAGCCCCTC ATACCATTGG 
CTAGAGACTA ACATGGATGC TGGAGAAAGG GAGGAGACAG AGGAAGAGGA GGAAGAAGAA 
GATGAAAACA TATGTGTGTG TGCAGGCCTT CCAGACTCTT CGATGGGTGT CCTCTATGGC 
CATGGCGGCC TGCATCTGAG AGCCATGGAG CCTGAGACCA AGACTCCATC CCAGGCTAGC 
GAGATTCTGC TGTCACCTCA AATACAGAAA GCACTAGAAG GTGTACACTA CATTGCTGAC 
CGTCTGAGGT CTGAGGATGC TGACTCTTCG GTGAAGGAAG ACTGGAAGTA TGTGGCCATG 
GTGGTAGACC GGATATTCCT CTGGCTGTTC ATTATCGTCT GCTTCCTGGG GACCATCGGA 
CTCTTCCTTC CTCCATTCCT GGCTGGAATG ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Developmental biology359(2)303-20(2011 Nov)
Richard AF,Demignon J,Sakakibara I,Pujol J,Favier M,Strochlic L,Le Grand F,Sgarioto N,Guernec A,Schmitt A,Cagnard N,Huang R,Legay C,Guillet-Deniau I,Maire P

Alpha2 nicotine receptors function as a molecular switch to continuously excite a subset of interneurons in rat hippocampal circuits.
The European journal of neuroscience29(8)1588-603(2009 Apr)
Jia Y,Yamazaki Y,Nakauchi S,Sumikawa K

Mammalian nicotinic acetylcholine receptors: from structure to function.
Physiological reviews89(1)73-120(2009 Jan)
Albuquerque EX,Pereira EF,Alkondon M,Rogers SW

Postnatal expression of alpha2 nicotinic acetylcholine receptor subunit mRNA in developing cortex and hippocampus.
Journal of chemical neuroanatomy32(2-4)179-90(2006 Dec)
Son JH,Winzer-Serhan UH

Nicotinic cholinergic receptors in the rat retina: simple and mixed heteromeric subtypes.
Molecular pharmacology68(6)1656-68(2005 Dec)
Marritt AM,Cox BC,Yasuda RP,McIntosh JM,Xiao Y,Wolfe BB,Kellar KJ