Ilk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ilk
Entrez Gene ID	170922
Full Name	integrin-linked kinase
General protein information	Preferred Names integrin-linked kinase Names integrin-linked protein kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	serine/threonine kinase involved in intracellular signal transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133409.2	NP_596900.1	integrin-linked protein kinase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ILK	XP_534040.1
Rattus norvegicus (Norway rat)	Ilk	NP_596900.1
	ILK	XP_001109041.1
Gallus gallus (chicken)	ILK	NP_989525.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006573	XP_316598.2
Pan troglodytes (chimpanzee)	ILK	XP_003312944.1
Mus musculus (house mouse)	Ilk	NP_001155196.1
Xenopus tropicalis (tropical clawed frog)	ilk	NP_001120512.1
Danio rerio (zebrafish)	ilk	NP_956865.1
Homo sapiens (human)	ILK	NP_004508.1
Bos taurus (cattle)	ILK	NP_001029865.1
Drosophila melanogaster (fruit fly)	Ilk	NP_525001.2
Caenorhabditis elegans (roundworm)	pat-4	NP_497139.1

Gene Ontology
Bibliography

Related articles in PubMed

ILK-PI3K/AKT pathway participates in cutaneous wound contraction by regulating fibroblast migration and differentiation to myofibroblast.
Li G, Li YY, Sun JE, Lin WH, Zhou RX
Laboratory investigation; a journal of technical methods and pathology96(7)741-51(2016 Jul)

Hyperphosphatemia induces cellular senescence in human aorta smooth muscle cells through integrin linked kinase (ILK) up-regulation.
Troyano N, Nogal MD, Mora I, Diaz-Naves M, Lopez-Carrillo N, Sosa P, Rodriguez-Puyol D, Olmos G, Ruiz-Torres MP
Mechanisms of ageing and development15243-55(2015 Dec)

Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells.
Moraes JA, Frony AC, Dias AM, Renovato-Martins M, Rodrigues G, Marcinkiewicz C, Assreuy J, Barja-Fidalgo C
Atherosclerosis243(2)477-85(2015 Dec)

Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury.
Yan G, Wang Q, Hu S, Wang D, Qiao Y, Ma G, Tang C, Gu Y
International journal of molecular medicine36(4)1001-11(2015 Oct)

Impaired ILK Function Is Associated with Deficits in Hippocampal Based Memory and Synaptic Plasticity in a FASD Rat Model.
Bhattacharya D, Dunaway EP, Bhattacharya S, Bloemer J, Buabeid M, Escobar M, Suppiramaniam V, Dhanasekaran M
PloS one10(8)e0135700(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Investigated the role of ILK in cutaneous wound contraction. We found that ILK was involved in cutaneous wound healing, and ILK and PI3K/AKT inhibitors inhibited wound contraction and re-epithelialization, consequently delaying wound healing in vivo.
Title: ILK-PI3K/AKT pathway participates in cutaneous wound contraction by regulating fibroblast migration and differentiation to myofibroblast.

high extracellular concentration of phosphate induced senescence in cultured smooth muscle through the activation of IGF-1 receptor and ILK overexpression
Title: Hyperphosphatemia induces cellular senescence in human aorta smooth muscle cells through integrin linked kinase (ILK) up-regulation.

Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects on vascular smooth muscle cell proliferation/migration.
Title: Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells.

These effects of digoxin were shown to be mediated, at least in part, through an increase in ILK/Akt signaling and a decrease in GSK-3beta signaling in PDGFBB-stimulated VSMCs
Title: Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury.

The ILK pathway appears to play a significant role in memory and synaptic plasticity impairments in FASD rats. These impairments appear to be mediated by reduced GSK3beta regulation
Title: Impaired ILK Function Is Associated with Deficits in Hippocampal Based Memory and Synaptic Plasticity in a FASD Rat Model.

The following Ilk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ilk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00710	NM_133409.2 Latest version!	Rattus norvegicus integrin-linked kinase (Ilk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ilk ( NM_133409.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596900.1 Rattus norvegicus integrin-linked kinase (Ilk), mRNA.

Price & Availability↑

CloneID	ORa00710
Clone ID Related Accession (Same CDS sequence)	NM_133409.2
Accession Version	NM_133409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	integrin-linked protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062406.1. On Dec 20, 2003 this sequence version replaced NM_133409.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACGACA TTTTCACTCA GTGCCGGGAG GGCAACGCGG TGGCGGTGCG CTTGTGGCTG 
GACAACACAG AGAACGACCT AAATCAGGGG GATGATCATG GCTTCTCCCC TTTGCACTGG 
GCCTGCCGAG AAGGCCGATC TGCAGTGGTT GAAATGCTGA TCATGCGGGG AGCACGGATC 
AATGTGATGA ATCGTGGGGA TGATACCCCC CTGCACTTGG CAGCTAGTCA CGGACACCGT 
GACATTGTAC AGAAGCTGTT GCAATACAAG GCTGACATCA ATGCAGTGAA TGAACATGGG 
AATGTGCCCC TTCACTATGC ATGTTTCTGG GGCCAAGACC AGGTGGCAGA GGACCTAGTA 
GCTAACGGGG CTCTTGTGAG CATCTGTAAC AAGTATGGAG AGATGCCTGT GGACAAAGCC 
AAGGCACCCC TTCGAGAGCT TCTCCGAGAA CGGGCAGAGA AGATGGGCCA GAATCTTAAT 
CGTATTCCAT ACAAGGACAC ATTCTGGAAG GGGACCACTC GCACAAGGCC CCGAAATGGG 
ACCCTGAACA AACACTCTGG TATTGACTTC AAACAGCTCA ACTTTCTGGC AAAGCTCAAC 
GAGAATCATT CTGGAGAGCT ATGGAAAGGC CGCTGGCAGG GCAATGACAT TGTTGTGAAG 
GTGCTGAAGG TTCGAGACTG GAGTACAAGG AAAAGCAGGG ACTTCAATGA GGAATGTCCC 
CGGCTCAGGA TTTTCTCTCA TCCTAACGTG CTTCCGGTAC TAGGTGCTTG CCAGGCTCCC 
CCAGCTCCCC ACCCAACCCT CATCACACAC TGGATGCCAT ATGGATCTCT CTACAATGTT 
CTACATGAAG GCACCAATTT CGTTGTGGAC CAGAGCCAAG CTGTAAAGTT TGCTTTGGAC 
ATGGCAAGAG GCATGGCTTT TCTTCATACA CTAGAGCCTC TCATACCTCG GCACGCACTC 
AATAGCCGTA GTGTAATGAT CGATGAAGAT ATGACTGCCC GAATCAGCAT GGCTGATGTA 
AAGTTTTCTT TCCAGTGCCC TGGGCGCATG TATGCACCTG CCTGGGTGGC CCCTGAAGCC 
CTGCAAAAGA AGCCTGAAGA TACAAACAGA CGCTCAGCAG ACATGTGGAG TTTTGCCGTG 
CTTCTGTGGG AACTGGTGAC ACGAGAGGTG CCCTTTGCTG ACCTTTCTAA TATGGAGATT 
GGAATGAAGG TGGCACTGGA AGGCCTTCGG CCTACTATCC CACCAGGTAT TTCCCCCCAC 
GTGTGTAAGC TCATGAAGAT TTGCATGAAT GAAGACCCTG CAAAGCGACC CAAGTTTGAC 
ATGATTGTAC CTATCTTGGA GAAGATGCAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596900.1
CDS	64..1422
Translation	MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLAKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQAPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK

Target ORF information:

RefSeq Version	NM_133409.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus integrin-linked kinase (Ilk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133409.2

ORF Insert Sequence:

ATGGACGACA TTTTCACTCA GTGCCGGGAG GGCAACGCGG TGGCGGTGCG CTTGTGGCTG 
GACAACACAG AGAACGACCT AAATCAGGGG GATGATCATG GCTTCTCCCC TTTGCACTGG 
GCCTGCCGAG AAGGCCGATC TGCAGTGGTT GAAATGCTGA TCATGCGGGG AGCACGGATC 
AATGTGATGA ATCGTGGGGA TGATACCCCC CTGCACTTGG CAGCTAGTCA CGGACACCGT 
GACATTGTAC AGAAGCTGTT GCAATACAAG GCTGACATCA ATGCAGTGAA TGAACATGGG 
AATGTGCCCC TTCACTATGC ATGTTTCTGG GGCCAAGACC AGGTGGCAGA GGACCTAGTA 
GCTAACGGGG CTCTTGTGAG CATCTGTAAC AAGTATGGAG AGATGCCTGT GGACAAAGCC 
AAGGCACCCC TTCGAGAGCT TCTCCGAGAA CGGGCAGAGA AGATGGGCCA GAATCTTAAT 
CGTATTCCAT ACAAGGACAC ATTCTGGAAG GGGACCACTC GCACAAGGCC CCGAAATGGG 
ACCCTGAACA AACACTCTGG TATTGACTTC AAACAGCTCA ACTTTCTGGC AAAGCTCAAC 
GAGAATCATT CTGGAGAGCT ATGGAAAGGC CGCTGGCAGG GCAATGACAT TGTTGTGAAG 
GTGCTGAAGG TTCGAGACTG GAGTACAAGG AAAAGCAGGG ACTTCAATGA GGAATGTCCC 
CGGCTCAGGA TTTTCTCTCA TCCTAACGTG CTTCCGGTAC TAGGTGCTTG CCAGGCTCCC 
CCAGCTCCCC ACCCAACCCT CATCACACAC TGGATGCCAT ATGGATCTCT CTACAATGTT 
CTACATGAAG GCACCAATTT CGTTGTGGAC CAGAGCCAAG CTGTAAAGTT TGCTTTGGAC 
ATGGCAAGAG GCATGGCTTT TCTTCATACA CTAGAGCCTC TCATACCTCG GCACGCACTC 
AATAGCCGTA GTGTAATGAT CGATGAAGAT ATGACTGCCC GAATCAGCAT GGCTGATGTA 
AAGTTTTCTT TCCAGTGCCC TGGGCGCATG TATGCACCTG CCTGGGTGGC CCCTGAAGCC 
CTGCAAAAGA AGCCTGAAGA TACAAACAGA CGCTCAGCAG ACATGTGGAG TTTTGCCGTG 
CTTCTGTGGG AACTGGTGAC ACGAGAGGTG CCCTTTGCTG ACCTTTCTAA TATGGAGATT 
GGAATGAAGG TGGCACTGGA AGGCCTTCGG CCTACTATCC CACCAGGTAT TTCCCCCCAC 
GTGTGTAAGC TCATGAAGAT TTGCATGAAT GAAGACCCTG CAAAGCGACC CAAGTTTGAC 
ATGATTGTAC CTATCTTGGA GAAGATGCAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ILK-PI3K/AKT pathway participates in cutaneous wound contraction by regulating fibroblast migration and differentiation to myofibroblast.
Laboratory investigation; a journal of technical methods and pathology96(7)741-51(2016 Jul)
Li G,Li YY,Sun JE,Lin WH,Zhou RX

Hyperphosphatemia induces cellular senescence in human aorta smooth muscle cells through integrin linked kinase (ILK) up-regulation.
Mechanisms of ageing and development15243-55(2015 Dec)
Troyano N,Nogal MD,Mora I,Diaz-Naves M,Lopez-Carrillo N,Sosa P,Rodriguez-Puyol D,Olmos G,Ruiz-Torres MP

Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells.
Atherosclerosis243(2)477-85(2015 Dec)
Moraes JA,Frony AC,Dias AM,Renovato-Martins M,Rodrigues G,Marcinkiewicz C,Assreuy J,Barja-Fidalgo C

Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury.
International journal of molecular medicine36(4)1001-11(2015 Oct)
Yan G,Wang Q,Hu S,Wang D,Qiao Y,Ma G,Tang C,Gu Y

Impaired ILK Function Is Associated with Deficits in Hippocampal Based Memory and Synaptic Plasticity in a FASD Rat Model.
PloS one10(8)e0135700(2015)
Bhattacharya D,Dunaway EP,Bhattacharya S,Bloemer J,Buabeid M,Escobar M,Suppiramaniam V,Dhanasekaran M

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Ilk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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