Prdm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prdm4
Entrez Gene ID	170820
Full Name	PR/SET domain 4
Synonyms	SC-1
General protein information	Preferred Names PR/SET domain 4 Names PR domain zinc finger protein 4 PR domain 4 PR domain containing 4 PR domain-containing protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	binds the cytoplasmic domain of the p75 neurotrophin receptor; may play a role in growth arrest [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241160.3	XP_006241222.1	PR domain zinc finger protein 4 isoform X1
XM_006241161.2	XP_006241223.1	PR domain zinc finger protein 4 isoform X2
XM_008765213.2	XP_008763435.1	PR domain zinc finger protein 4 isoform X3
NM_133312.1	NP_579846.1	PR domain zinc finger protein 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prdm4	NP_579846.1
Danio rerio (zebrafish)	prdm4	NP_001116215.1
Pan troglodytes (chimpanzee)	PRDM4	XP_001162965.1
	PRDM4	XP_001101382.1
Canis lupus familiaris (dog)	PRDM4	XP_538412.2
Bos taurus (cattle)	PRDM4	XP_005206854.1
Xenopus tropicalis (tropical clawed frog)	prdm4	NP_001072257.1
Homo sapiens (human)	PRDM4	NP_036538.3
Mus musculus (house mouse)	Prdm4	NP_857633.2
Gallus gallus (chicken)	PRDM4	XP_416301.4

Gene Ontology
Bibliography

Related articles in PubMed

Zinc fingers 1, 2, 5 and 6 of transcriptional regulator, PRDM4, are required for its nuclear localisation.
Tunbak H, Georgiou C, Guan C, Richardson WD, Chittka A
Biochemical and biophysical research communications474(2)388-394(2016 05)

The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo.
Bogani D, Morgan MA, Nelson AC, Costello I, McGouran JF, Kessler BM, Robertson EJ, Bikoff EK
Molecular and cellular biology33(19)3936-50(2013 Oct)

Transcription factor positive regulatory domain 4 (PRDM4) recruits protein arginine methyltransferase 5 (PRMT5) to mediate histone arginine methylation and control neural stem cell proliferation and differentiation.
Chittka A, Nitarska J, Grazini U, Richardson WD
The Journal of biological chemistry287(51)42995-3006(2012 Dec)

The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E.
Chittka A, Arevalo JC, Rodriguez-Guzman M, P?rez P, Chao MV, Sendtner M
The Journal of cell biology164(7)985-96(2004 Mar)

Identification of a zinc finger protein whose subcellular distribution is regulated by serum and nerve growth factor.
Chittka A, Chao MV
Proceedings of the National Academy of Sciences of the United States of America96(19)10705-10(1999 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We investigated the potential mechanism of nuclear shuttling of PRDM4 via the importin alpha/beta-mediated pathway and find that PRDM4 nuclear targeting is independent of alpha/beta-mediated nuclear import.
Title: Zinc fingers 1, 2, 5 and 6 of transcriptional regulator, PRDM4, are required for its nuclear localisation.

SC1 and PRMT5 are components of an epigenetic regulatory complex that maintains the "stem-like" cellular state of the neural stem cells.
Title: Transcription factor positive regulatory domain 4 (PRDM4) recruits protein arginine methyltransferase 5 (PRMT5) to mediate histone arginine methylation and control neural stem cell proliferation and differentiation.

The following Prdm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prdm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa44238	XM_008765213.2 Latest version!	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa00594	NM_133312.1 Latest version!	Rattus norvegicus PR/SET domain 4 (Prdm4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$468.30 $669.00
ORa30009	XM_006241160.3 Latest version!	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa30010	XM_006241161.2 Latest version!	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prdm4 ( XM_008765213.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763435.1 Rattus norvegicus PR domain 4 (Prdm4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44238
Clone ID Related Accession (Same CDS sequence)	XM_008765213.2
Accession Version	XM_008765213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	PR domain zinc finger protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGTTGGTG TCCTTCCGTT GATAGGCGTG TGGACTGCAG AGACCATTCC CGTGCGGACT 
TGCTTCGGGC CTCTAATTGG CCAGCAGAGT CACTCCATGG AAGTGGCGGA GTGGACAGAC 
AAGGCCGTTA GCCACGTCTG GAAGATATAC CACAATGGCG TCCTAGAATT CTGCATCATT 
ACAACCGATG AGAATGAGTG TAATTGGATG ATGTTTGTGC GCAAAGCCAG GAACCGTGAA 
GAACAGAATT TGGTGGCTTA TCCCCATGAT GGAAAAATCT ATTTCTGTAC CTCACAAGAC 
ATCCCTCCTG AAAACGAGCT GCTTTTCTAT TATAGCCGGG ATTATGCTCA ACAGATAGGT 
GTTCCTGAAC ACCCCGATGT GCACCTCTGT AACTGTGGCA AGGAGTGCAG TTCCTATTCA 
GAGTTCAAAG CTCACCTGAC CAGCCATATC CACAATCATC TCCCTAGCCA GGGCCACAGC 
AGCAGCCATG GGCCAAGCCA CAGCAAGGAA AGGAAGTGGA AGTGCTCCAT GTGCCCCCAG 
GCCTTTATTT CTCCTTCTAA ACTCCACGTT CACTTTATGG GTCATATGGG CATGAAGCCC 
CACAAGTGTG ATTTTTGTAG CAAGGCTTTT AGTGATCCAA GCAACCTACG GACACACCTC 
AAAATACATA CAGTGTGGTG TCCTGGAATG ATTTGCAGGT CAGAAGAATT ACAGGTGTAC 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763435.1
CDS	465..2453
Translation	MNDMNLSPVGMEQLSSSSVSNALPVSGSHLGLAASPSHSAIPAPGLPVAIPNLGPSLSSLPSALSLMLPMGIGDRGVMCGLPERNYTLPPPPYPHLESSYFRTILPGILSYLADRPPPQYIHPNSINVDGNTALSITNNPSALDPYQANGNVGLELGIVSIDSRSVNTHGAQSLHPNDGHEVALDTTITMENVSRVTSPISTDGMAEELTMDGVTGEHSQIPNGSRSHEPLSVDSVSNNLTADTVGHGGVIPIHGNGLELPVVMETDHIANRVNGISDSVLSDSIHTVAMSTNSVSVALSTSHNLASLESVSLQEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVPSSLQMEDSNSNKENMATLFTIWCTLCDRAYPSDCPDHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGTDVVGVLPLIGVWTAETIPVRTCFGPLIGQQSHSMEVAEWTDKAVSHVWKIYHNGVLEFCIITTDENECNWMMFVRKARNREEQNLVAYPHDGKIYFCTSQDIPPENELLFYYSRDYAQQIGVPEHPDVHLCNCGKECSSYSEFKAHLTSHIHNHLPSQGHSSSHGPSHSKERKWKCSMCPQAFISPSKLHVHFMGHMGMKPHKCDFCSKAFSDPSNLRTHLKIHTVWCPGMICRSEELQVYFV

Target ORF information:

RefSeq Version	XM_008765213.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765213.2

ORF Insert Sequence:

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGTTGGTG TCCTTCCGTT GATAGGCGTG TGGACTGCAG AGACCATTCC CGTGCGGACT 
TGCTTCGGGC CTCTAATTGG CCAGCAGAGT CACTCCATGG AAGTGGCGGA GTGGACAGAC 
AAGGCCGTTA GCCACGTCTG GAAGATATAC CACAATGGCG TCCTAGAATT CTGCATCATT 
ACAACCGATG AGAATGAGTG TAATTGGATG ATGTTTGTGC GCAAAGCCAG GAACCGTGAA 
GAACAGAATT TGGTGGCTTA TCCCCATGAT GGAAAAATCT ATTTCTGTAC CTCACAAGAC 
ATCCCTCCTG AAAACGAGCT GCTTTTCTAT TATAGCCGGG ATTATGCTCA ACAGATAGGT 
GTTCCTGAAC ACCCCGATGT GCACCTCTGT AACTGTGGCA AGGAGTGCAG TTCCTATTCA 
GAGTTCAAAG CTCACCTGAC CAGCCATATC CACAATCATC TCCCTAGCCA GGGCCACAGC 
AGCAGCCATG GGCCAAGCCA CAGCAAGGAA AGGAAGTGGA AGTGCTCCAT GTGCCCCCAG 
GCCTTTATTT CTCCTTCTAA ACTCCACGTT CACTTTATGG GTCATATGGG CATGAAGCCC 
CACAAGTGTG ATTTTTGTAG CAAGGCTTTT AGTGATCCAA GCAACCTACG GACACACCTC 
AAAATACATA CAGTGTGGTG TCCTGGAATG ATTTGCAGGT CAGAAGAATT ACAGGTGTAC 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prdm4 ( NM_133312.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_579846.1 Rattus norvegicus PR/SET domain 4 (Prdm4), mRNA.

Price & Availability↑

CloneID	ORa00594
Clone ID Related Accession (Same CDS sequence)	NM_133312.1
Accession Version	NM_133312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	PR domain zinc finger protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF176023.1. ##Evidence-Data-START## Transcript exon combination :: AF176023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACTTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCATAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGGCGTGT GGACTGCAGA GACCATTCCC GTGCGGACTT GCTTCGGGCC TCTAATTGGC 
CAGCAGAGTC ACTCCATGGA AGTGGCGGAG TGGACAGACA AGGCCGTTAG CCACGTCTGG 
AAGATATACC ACAATGGCGT CCTAGAATTC TGCATCATTA CAACCGATGA GAATGAGTGT 
AATTGGATGA TGTTTGTGCG CAAAGCCAGG AACCGTGAAG AACAGAATTT GGTGGCTTAT 
CCCCATGATG GAAAAATCTA TTTCTGTACC TCACAAGACA TCCCTCCTGA AAACGAGCTG 
CTTTTCTATT ATAGCCGGGA TTATGCTCAA CAGATAGGTG TTCCTGAACA CCCCGATGTG 
CACCTCTGTA ACTGTGGCAA GGAGTGCAGT TCCTATTCAG AGTTCAAAGC TCACCTGACC 
AGCCATATCC ACAATCATCT CCCTAGCCAG GGCCACAGCA GCAGCCATGG GCCAAGCCAC 
AGCAAGGAAA GGAAGTGGAA GTGCTCCATG TGCCCCCAGG CCTTTATTTC TCCTTCTAAA 
CTCCACGTTC ACTTTATGGG TCATATGGGC ATGAAGCCCC ACAAGTGTGA TTTTTGTAGC 
AAGGCTTTTA GTGATCCAAG CAACCTACGG ACACACCTCA AAATACATAC AGGTCAGAAG 
AATTACAGGT GTACTTTGTG TGACAAGTCT TTCACGCAGA AGGCTCACTT GGAGTCTCAC 
ATGGTCATCC ACACGGGTGA GAAGAATCTC AAGTGTGATT ACTGTGACAA GCTATTCATG 
CGGAGACAGG ACCTCAAGCA GCATGTGCTC ATCCACACAC AAGAACGCCA GATCAAGTGT 
CCGAAGTGTG ATAAACTGTT CCTGAGAACA AACCACTTGA AGAAGCATCT CAACTCTCAC 
GAGGGAAGAC GAGACTATGT CTGTGAGAAA TGCACAAAGG CTTACCTAAC CAAGTACCAC 
CTCACCCGCC ACCTGAAGGC CTGCAAGGAG CCCGCCTCCA GCTCCTCCGC CCAGGACGAT 
GAGGACGAGG ACGGGGACTC GGGGGAAGAC GGTCTCCCAG GCTCCATGAC GACAGAGGGC 
TGCAGGATGA GCAGTGCTGT TTACTCAGCA GATGAGTCTC TCTCCGCACA TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579846.1
CDS	1..2397
Translation	MNDMNLSPVGMEQLSSSSVSNALPVSGSHLGLAASPSHSAIPAPGLPVAIPNLGPSLSSLPSALSLMLPMGIGDRGVMCGLPERNYTLPPPPYPHLESSYFRTILPGILSYLADRPPPQYIHPNSINVDGNTALSITNNPSALDPYQANGNVGLELGIVSIDSRSVNTHGAQSLHPNDGHEVALDTTITMENVSRVTSPISTDGMAEELTMDGVTGEHSQIPNGSRSHEPLSVDSVSNNLTADTVGHGGVIPIHGNGLELPVVMETDHIANRVNGISDSVLSDSIHTVAMSTNSVSVALSTSHNFASLESVSLQEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVPSSLQMEDSNSNKENMATLFTIWCTLCDRAYPSDCPDHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGTDVGVWTAETIPVRTCFGPLIGQQSHSMEVAEWTDKAVSHVWKIYHNGVLEFCIITTDENECNWMMFVRKARNREEQNLVAYPHDGKIYFCTSQDIPPENELLFYYSRDYAQQIGVPEHPDVHLCNCGKECSSYSEFKAHLTSHIHNHLPSQGHSSSHGPSHSKERKWKCSMCPQAFISPSKLHVHFMGHMGMKPHKCDFCSKAFSDPSNLRTHLKIHTGQKNYRCTLCDKSFTQKAHLESHMVIHTGEKNLKCDYCDKLFMRRQDLKQHVLIHTQERQIKCPKCDKLFLRTNHLKKHLNSHEGRRDYVCEKCTKAYLTKYHLTRHLKACKEPASSSSAQDDEDEDGDSGEDGLPGSMTTEGCRMSSAVYSADESLSAHK

Target ORF information:

RefSeq Version	NM_133312.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PR/SET domain 4 (Prdm4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133312.1

ORF Insert Sequence:

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACTTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCATAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGGCGTGT GGACTGCAGA GACCATTCCC GTGCGGACTT GCTTCGGGCC TCTAATTGGC 
CAGCAGAGTC ACTCCATGGA AGTGGCGGAG TGGACAGACA AGGCCGTTAG CCACGTCTGG 
AAGATATACC ACAATGGCGT CCTAGAATTC TGCATCATTA CAACCGATGA GAATGAGTGT 
AATTGGATGA TGTTTGTGCG CAAAGCCAGG AACCGTGAAG AACAGAATTT GGTGGCTTAT 
CCCCATGATG GAAAAATCTA TTTCTGTACC TCACAAGACA TCCCTCCTGA AAACGAGCTG 
CTTTTCTATT ATAGCCGGGA TTATGCTCAA CAGATAGGTG TTCCTGAACA CCCCGATGTG 
CACCTCTGTA ACTGTGGCAA GGAGTGCAGT TCCTATTCAG AGTTCAAAGC TCACCTGACC 
AGCCATATCC ACAATCATCT CCCTAGCCAG GGCCACAGCA GCAGCCATGG GCCAAGCCAC 
AGCAAGGAAA GGAAGTGGAA GTGCTCCATG TGCCCCCAGG CCTTTATTTC TCCTTCTAAA 
CTCCACGTTC ACTTTATGGG TCATATGGGC ATGAAGCCCC ACAAGTGTGA TTTTTGTAGC 
AAGGCTTTTA GTGATCCAAG CAACCTACGG ACACACCTCA AAATACATAC AGGTCAGAAG 
AATTACAGGT GTACTTTGTG TGACAAGTCT TTCACGCAGA AGGCTCACTT GGAGTCTCAC 
ATGGTCATCC ACACGGGTGA GAAGAATCTC AAGTGTGATT ACTGTGACAA GCTATTCATG 
CGGAGACAGG ACCTCAAGCA GCATGTGCTC ATCCACACAC AAGAACGCCA GATCAAGTGT 
CCGAAGTGTG ATAAACTGTT CCTGAGAACA AACCACTTGA AGAAGCATCT CAACTCTCAC 
GAGGGAAGAC GAGACTATGT CTGTGAGAAA TGCACAAAGG CTTACCTAAC CAAGTACCAC 
CTCACCCGCC ACCTGAAGGC CTGCAAGGAG CCCGCCTCCA GCTCCTCCGC CCAGGACGAT 
GAGGACGAGG ACGGGGACTC GGGGGAAGAC GGTCTCCCAG GCTCCATGAC GACAGAGGGC 
TGCAGGATGA GCAGTGCTGT TTACTCAGCA GATGAGTCTC TCTCCGCACA TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prdm4 ( XM_006241160.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241222.1 Rattus norvegicus PR domain 4 (Prdm4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30009
Clone ID Related Accession (Same CDS sequence)	XM_006241160.3
Accession Version	XM_006241160.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	PR domain zinc finger protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGTTGGTG TCCTTCCGTT GATAGGCGTG TGGACTGCAG AGACCATTCC CGTGCGGACT 
TGCTTCGGGC CTCTAATTGG CCAGCAGAGT CACTCCATGG AAGTGGCGGA GTGGACAGAC 
AAGGCCGTTA GCCACGTCTG GAAGATATAC CACAATGGCG TCCTAGAATT CTGCATCATT 
ACAACCGATG AGAATGAGTG TAATTGGATG ATGTTTGTGC GCAAAGCCAG GAACCGTGAA 
GAACAGAATT TGGTGGCTTA TCCCCATGAT GGAAAAATCT ATTTCTGTAC CTCACAAGAC 
ATCCCTCCTG AAAACGAGCT GCTTTTCTAT TATAGCCGGG ATTATGCTCA ACAGATAGGT 
GTTCCTGAAC ACCCCGATGT GCACCTCTGT AACTGTGGCA AGGAGTGCAG TTCCTATTCA 
GAGTTCAAAG CTCACCTGAC CAGCCATATC CACAATCATC TCCCTAGCCA GGGCCACAGC 
AGCAGCCATG GGCCAAGCCA CAGCAAGGAA AGGAAGTGGA AGTGCTCCAT GTGCCCCCAG 
GCCTTTATTT CTCCTTCTAA ACTCCACGTT CACTTTATGG GTCATATGGG CATGAAGCCC 
CACAAGTGTG ATTTTTGTAG CAAGGCTTTT AGTGATCCAA GCAACCTACG GACACACCTC 
AAAATACATA CAGGTCAGAA GAATTACAGG TGTACTTTGT GTGACAAGTC TTTCACGCAG 
AAGGCTCACT TGGAATCTCA CATGGTCATC CACACGGGTG AGAAGAATCT CAAGTGTGAT 
TACTGTGACA AGCTATTCAT GCGGAGACAG GACCTCAAGC AGCATGTGCT CATCCACACA 
CAAGAACGCC AGATCAAGTG TCCGAAGTGT GATAAACTGT TCCTGAGAAC AAACCACTTG 
AAGAAGCATC TCAACTCTCA CGAGGGAAGA CGAGACTATG TCTGTGAGAA ATGCACAAAG 
GCTTACCTAA CCAAGTACCA CCTCACCCGC CACCTGAAGG CCTGCAAGGA GCCCGCCTCC 
AGCTCCTCCG CCCAGGACGA TGAGGACGAG GATGGGGACT CGGGGGAAGA CGGTCTCCCA 
GGCTCCATGA CGACAGAGGG CTGCAGGATG AGCAGTGCTG TTTACTCAGC AGATGAGTCT 
CTCTCCGCAC ATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241222.1
CDS	462..2879
Translation	MNDMNLSPVGMEQLSSSSVSNALPVSGSHLGLAASPSHSAIPAPGLPVAIPNLGPSLSSLPSALSLMLPMGIGDRGVMCGLPERNYTLPPPPYPHLESSYFRTILPGILSYLADRPPPQYIHPNSINVDGNTALSITNNPSALDPYQANGNVGLELGIVSIDSRSVNTHGAQSLHPNDGHEVALDTTITMENVSRVTSPISTDGMAEELTMDGVTGEHSQIPNGSRSHEPLSVDSVSNNLTADTVGHGGVIPIHGNGLELPVVMETDHIANRVNGISDSVLSDSIHTVAMSTNSVSVALSTSHNLASLESVSLQEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVPSSLQMEDSNSNKENMATLFTIWCTLCDRAYPSDCPDHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGTDVVGVLPLIGVWTAETIPVRTCFGPLIGQQSHSMEVAEWTDKAVSHVWKIYHNGVLEFCIITTDENECNWMMFVRKARNREEQNLVAYPHDGKIYFCTSQDIPPENELLFYYSRDYAQQIGVPEHPDVHLCNCGKECSSYSEFKAHLTSHIHNHLPSQGHSSSHGPSHSKERKWKCSMCPQAFISPSKLHVHFMGHMGMKPHKCDFCSKAFSDPSNLRTHLKIHTGQKNYRCTLCDKSFTQKAHLESHMVIHTGEKNLKCDYCDKLFMRRQDLKQHVLIHTQERQIKCPKCDKLFLRTNHLKKHLNSHEGRRDYVCEKCTKAYLTKYHLTRHLKACKEPASSSSAQDDEDEDGDSGEDGLPGSMTTEGCRMSSAVYSADESLSAHK

Target ORF information:

RefSeq Version	XM_006241160.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241160.3

ORF Insert Sequence:

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGTTGGTG TCCTTCCGTT GATAGGCGTG TGGACTGCAG AGACCATTCC CGTGCGGACT 
TGCTTCGGGC CTCTAATTGG CCAGCAGAGT CACTCCATGG AAGTGGCGGA GTGGACAGAC 
AAGGCCGTTA GCCACGTCTG GAAGATATAC CACAATGGCG TCCTAGAATT CTGCATCATT 
ACAACCGATG AGAATGAGTG TAATTGGATG ATGTTTGTGC GCAAAGCCAG GAACCGTGAA 
GAACAGAATT TGGTGGCTTA TCCCCATGAT GGAAAAATCT ATTTCTGTAC CTCACAAGAC 
ATCCCTCCTG AAAACGAGCT GCTTTTCTAT TATAGCCGGG ATTATGCTCA ACAGATAGGT 
GTTCCTGAAC ACCCCGATGT GCACCTCTGT AACTGTGGCA AGGAGTGCAG TTCCTATTCA 
GAGTTCAAAG CTCACCTGAC CAGCCATATC CACAATCATC TCCCTAGCCA GGGCCACAGC 
AGCAGCCATG GGCCAAGCCA CAGCAAGGAA AGGAAGTGGA AGTGCTCCAT GTGCCCCCAG 
GCCTTTATTT CTCCTTCTAA ACTCCACGTT CACTTTATGG GTCATATGGG CATGAAGCCC 
CACAAGTGTG ATTTTTGTAG CAAGGCTTTT AGTGATCCAA GCAACCTACG GACACACCTC 
AAAATACATA CAGGTCAGAA GAATTACAGG TGTACTTTGT GTGACAAGTC TTTCACGCAG 
AAGGCTCACT TGGAATCTCA CATGGTCATC CACACGGGTG AGAAGAATCT CAAGTGTGAT 
TACTGTGACA AGCTATTCAT GCGGAGACAG GACCTCAAGC AGCATGTGCT CATCCACACA 
CAAGAACGCC AGATCAAGTG TCCGAAGTGT GATAAACTGT TCCTGAGAAC AAACCACTTG 
AAGAAGCATC TCAACTCTCA CGAGGGAAGA CGAGACTATG TCTGTGAGAA ATGCACAAAG 
GCTTACCTAA CCAAGTACCA CCTCACCCGC CACCTGAAGG CCTGCAAGGA GCCCGCCTCC 
AGCTCCTCCG CCCAGGACGA TGAGGACGAG GATGGGGACT CGGGGGAAGA CGGTCTCCCA 
GGCTCCATGA CGACAGAGGG CTGCAGGATG AGCAGTGCTG TTTACTCAGC AGATGAGTCT 
CTCTCCGCAC ATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prdm4 ( XM_006241161.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241223.1 Rattus norvegicus PR domain 4 (Prdm4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30010
Clone ID Related Accession (Same CDS sequence)	XM_006241161.2
Accession Version	XM_006241161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	PR domain zinc finger protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGGCGTGT GGACTGCAGA GACCATTCCC GTGCGGACTT GCTTCGGGCC TCTAATTGGC 
CAGCAGAGTC ACTCCATGGA AGTGGCGGAG TGGACAGACA AGGCCGTTAG CCACGTCTGG 
AAGATATACC ACAATGGCGT CCTAGAATTC TGCATCATTA CAACCGATGA GAATGAGTGT 
AATTGGATGA TGTTTGTGCG CAAAGCCAGG AACCGTGAAG AACAGAATTT GGTGGCTTAT 
CCCCATGATG GAAAAATCTA TTTCTGTACC TCACAAGACA TCCCTCCTGA AAACGAGCTG 
CTTTTCTATT ATAGCCGGGA TTATGCTCAA CAGATAGGTG TTCCTGAACA CCCCGATGTG 
CACCTCTGTA ACTGTGGCAA GGAGTGCAGT TCCTATTCAG AGTTCAAAGC TCACCTGACC 
AGCCATATCC ACAATCATCT CCCTAGCCAG GGCCACAGCA GCAGCCATGG GCCAAGCCAC 
AGCAAGGAAA GGAAGTGGAA GTGCTCCATG TGCCCCCAGG CCTTTATTTC TCCTTCTAAA 
CTCCACGTTC ACTTTATGGG TCATATGGGC ATGAAGCCCC ACAAGTGTGA TTTTTGTAGC 
AAGGCTTTTA GTGATCCAAG CAACCTACGG ACACACCTCA AAATACATAC AGGTCAGAAG 
AATTACAGGT GTACTTTGTG TGACAAGTCT TTCACGCAGA AGGCTCACTT GGAATCTCAC 
ATGGTCATCC ACACGGGTGA GAAGAATCTC AAGTGTGATT ACTGTGACAA GCTATTCATG 
CGGAGACAGG ACCTCAAGCA GCATGTGCTC ATCCACACAC AAGAACGCCA GATCAAGTGT 
CCGAAGTGTG ATAAACTGTT CCTGAGAACA AACCACTTGA AGAAGCATCT CAACTCTCAC 
GAGGGAAGAC GAGACTATGT CTGTGAGAAA TGCACAAAGG CTTACCTAAC CAAGTACCAC 
CTCACCCGCC ACCTGAAGGC CTGCAAGGAG CCCGCCTCCA GCTCCTCCGC CCAGGACGAT 
GAGGACGAGG ATGGGGACTC GGGGGAAGAC GGTCTCCCAG GCTCCATGAC GACAGAGGGC 
TGCAGGATGA GCAGTGCTGT TTACTCAGCA GATGAGTCTC TCTCCGCACA TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241223.1
CDS	462..2858
Translation	MNDMNLSPVGMEQLSSSSVSNALPVSGSHLGLAASPSHSAIPAPGLPVAIPNLGPSLSSLPSALSLMLPMGIGDRGVMCGLPERNYTLPPPPYPHLESSYFRTILPGILSYLADRPPPQYIHPNSINVDGNTALSITNNPSALDPYQANGNVGLELGIVSIDSRSVNTHGAQSLHPNDGHEVALDTTITMENVSRVTSPISTDGMAEELTMDGVTGEHSQIPNGSRSHEPLSVDSVSNNLTADTVGHGGVIPIHGNGLELPVVMETDHIANRVNGISDSVLSDSIHTVAMSTNSVSVALSTSHNLASLESVSLQEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVPSSLQMEDSNSNKENMATLFTIWCTLCDRAYPSDCPDHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGTDVGVWTAETIPVRTCFGPLIGQQSHSMEVAEWTDKAVSHVWKIYHNGVLEFCIITTDENECNWMMFVRKARNREEQNLVAYPHDGKIYFCTSQDIPPENELLFYYSRDYAQQIGVPEHPDVHLCNCGKECSSYSEFKAHLTSHIHNHLPSQGHSSSHGPSHSKERKWKCSMCPQAFISPSKLHVHFMGHMGMKPHKCDFCSKAFSDPSNLRTHLKIHTGQKNYRCTLCDKSFTQKAHLESHMVIHTGEKNLKCDYCDKLFMRRQDLKQHVLIHTQERQIKCPKCDKLFLRTNHLKKHLNSHEGRRDYVCEKCTKAYLTKYHLTRHLKACKEPASSSSAQDDEDEDGDSGEDGLPGSMTTEGCRMSSAVYSADESLSAHK

Target ORF information:

RefSeq Version	XM_006241161.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PR domain 4 (Prdm4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241161.2

ORF Insert Sequence:

ATGAATGACA TGAACTTGAG CCCAGTGGGG ATGGAGCAGC TGTCTTCATC CTCTGTGAGC 
AATGCCCTGC CAGTCTCAGG AAGTCACCTG GGTCTGGCTG CCTCTCCCTC TCACAGTGCC 
ATCCCTGCCC CAGGACTTCC AGTGGCAATT CCAAACCTCG GTCCCTCCCT GAGCTCTCTG 
CCTTCTGCCT TGTCTCTGAT GCTCCCCATG GGCATTGGAG ATCGAGGAGT AATGTGTGGG 
TTACCTGAAA GAAACTATAC CCTACCTCCA CCACCTTACC CTCACCTAGA GAGCAGTTAC 
TTCAGAACCA TTCTACCTGG CATTTTATCT TATTTAGCTG ACAGACCACC TCCTCAGTAT 
ATCCACCCCA ACTCTATAAA TGTTGATGGA AATACAGCAT TATCTATCAC CAACAACCCT 
TCGGCACTAG ATCCCTATCA GGCCAATGGA AATGTTGGAT TAGAACTAGG CATTGTTTCA 
ATAGACTCTC GCTCTGTAAA CACTCATGGT GCCCAAAGTC TTCATCCTAA TGATGGCCAC 
GAAGTAGCAT TGGACACAAC AATCACTATG GAGAATGTCT CTAGGGTTAC CAGCCCAATC 
TCCACAGACG GAATGGCAGA GGAACTTACA ATGGATGGAG TTACAGGAGA ACATTCCCAG 
ATCCCAAATG GCTCCAGAAG TCATGAACCC CTCTCTGTGG ATTCTGTGAG CAACAACCTT 
ACAGCTGACA CTGTAGGACA TGGTGGTGTG ATACCCATTC ATGGGAATGG TCTGGAGCTT 
CCAGTGGTCA TGGAGACAGA CCACATTGCA AACCGGGTCA ATGGGATATC TGACAGCGTC 
CTCAGTGATT CCATCCACAC CGTGGCCATG AGCACCAACT CTGTAAGCGT GGCACTTTCT 
ACCTCACACA ACCTCGCCTC CCTAGAATCT GTTTCCCTCC AGGAAGTTGG CCTTAGCCTA 
GAGCCTGTGG CTGTCTCTTC CATCACCCAG GAAGTTGCCA TGGGGACAGG TCATGTAGAT 
GTATCCTCAG ACAGCTTGTC TTTTGTACCA TCTTCACTGC AAATGGAAGA CTCCAATTCA 
AACAAGGAAA ACATGGCAAC CTTGTTTACA ATTTGGTGCA CGCTTTGTGA CCGAGCCTAC 
CCCTCAGATT GCCCTGATCA TGGACCAGTG ACTTTTGTTC CTGATACACC AATAGAGAGC 
AGAGCAAGAC TATCCCTCCC GAAGCAGCTT GTTCTCCGCC AGTCCATTGT GGGGACAGAT 
GTTGGCGTGT GGACTGCAGA GACCATTCCC GTGCGGACTT GCTTCGGGCC TCTAATTGGC 
CAGCAGAGTC ACTCCATGGA AGTGGCGGAG TGGACAGACA AGGCCGTTAG CCACGTCTGG 
AAGATATACC ACAATGGCGT CCTAGAATTC TGCATCATTA CAACCGATGA GAATGAGTGT 
AATTGGATGA TGTTTGTGCG CAAAGCCAGG AACCGTGAAG AACAGAATTT GGTGGCTTAT 
CCCCATGATG GAAAAATCTA TTTCTGTACC TCACAAGACA TCCCTCCTGA AAACGAGCTG 
CTTTTCTATT ATAGCCGGGA TTATGCTCAA CAGATAGGTG TTCCTGAACA CCCCGATGTG 
CACCTCTGTA ACTGTGGCAA GGAGTGCAGT TCCTATTCAG AGTTCAAAGC TCACCTGACC 
AGCCATATCC ACAATCATCT CCCTAGCCAG GGCCACAGCA GCAGCCATGG GCCAAGCCAC 
AGCAAGGAAA GGAAGTGGAA GTGCTCCATG TGCCCCCAGG CCTTTATTTC TCCTTCTAAA 
CTCCACGTTC ACTTTATGGG TCATATGGGC ATGAAGCCCC ACAAGTGTGA TTTTTGTAGC 
AAGGCTTTTA GTGATCCAAG CAACCTACGG ACACACCTCA AAATACATAC AGGTCAGAAG 
AATTACAGGT GTACTTTGTG TGACAAGTCT TTCACGCAGA AGGCTCACTT GGAATCTCAC 
ATGGTCATCC ACACGGGTGA GAAGAATCTC AAGTGTGATT ACTGTGACAA GCTATTCATG 
CGGAGACAGG ACCTCAAGCA GCATGTGCTC ATCCACACAC AAGAACGCCA GATCAAGTGT 
CCGAAGTGTG ATAAACTGTT CCTGAGAACA AACCACTTGA AGAAGCATCT CAACTCTCAC 
GAGGGAAGAC GAGACTATGT CTGTGAGAAA TGCACAAAGG CTTACCTAAC CAAGTACCAC 
CTCACCCGCC ACCTGAAGGC CTGCAAGGAG CCCGCCTCCA GCTCCTCCGC CCAGGACGAT 
GAGGACGAGG ATGGGGACTC GGGGGAAGAC GGTCTCCCAG GCTCCATGAC GACAGAGGGC 
TGCAGGATGA GCAGTGCTGT TTACTCAGCA GATGAGTCTC TCTCCGCACA TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zinc fingers 1, 2, 5 and 6 of transcriptional regulator, PRDM4, are required for its nuclear localisation.
Biochemical and biophysical research communications474(2)388-394(2016 05)
Tunbak H,Georgiou C,Guan C,Richardson WD,Chittka A

The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo.
Molecular and cellular biology33(19)3936-50(2013 Oct)
Bogani D,Morgan MA,Nelson AC,Costello I,McGouran JF,Kessler BM,Robertson EJ,Bikoff EK

Transcription factor positive regulatory domain 4 (PRDM4) recruits protein arginine methyltransferase 5 (PRMT5) to mediate histone arginine methylation and control neural stem cell proliferation and differentiation.
The Journal of biological chemistry287(51)42995-3006(2012 Dec)
Chittka A,Nitarska J,Grazini U,Richardson WD

The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E.
The Journal of cell biology164(7)985-96(2004 Mar)
Chittka A,Arevalo JC,Rodriguez-Guzman M,P?rez P,Chao MV,Sendtner M

Identification of a zinc finger protein whose subcellular distribution is regulated by serum and nerve growth factor.
Proceedings of the National Academy of Sciences of the United States of America96(19)10705-10(1999 Sep)
Chittka A,Chao MV

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Prdm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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