The following Capn8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa44235 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006250363.3 | |
| Accession Version | XM_006250363.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | calpain-8 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCCCTTT GGAACAGAGC CTCCCTCAGG TGGAAAGTCT ACATGCCCCA CCTCCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006250425.2 |
| CDS | 326..1621 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006250363.3 |
1 | ATGTCCCTTT GGAACAGAGC CTCCCTCAGG TGGAAAGTCT ACATGCCCCA CCTCCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa00590 | |
| Clone ID Related Accession (Same CDS sequence) | NM_133309.2 | |
| Accession Version | NM_133309.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | calpain-8 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D14479.1. On Mar 5, 2003 this sequence version replaced NM_133309.1. ##Evidence-Data-START## Transcript exon combination :: D14479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCAGCTC TGGCAGCGGG AGTATCCAAG CAACGTGCAG TTGCTGAGGG GCTTGGCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_579843.2 |
| CDS | 51..2162 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_133309.2 |
1 | ATGGCAGCTC TGGCAGCGGG AGTATCCAAG CAACGTGCAG TTGCTGAGGG GCTTGGCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa57989 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017598654.1 | |
| Accession Version | XM_017598654.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | calpain-8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCCCTTT GGAACAGAGC CTCCCTCAGG TGGAAAGTCT ACATGCCCCA CCTCCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017454143.1 |
| CDS | 326..2029 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017598654.1 |
1 | ATGTCCCTTT GGAACAGAGC CTCCCTCAGG TGGAAAGTCT ACATGCCCCA CCTCCCTGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains. |
Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP. |
Estrogen regulates a tissue-specific calpain in the anterior pituitary. |
A novel tissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca(2+)-binding domain. |
