Acaa2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acaa2
Entrez Gene ID	170465
Full Name	acetyl-CoA acyltransferase 2
General protein information	Preferred Names acetyl-CoA acyltransferase 2 Names 3-ketoacyl-CoA thiolase, mitochondrial acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) beta-ketothiolase mitochondrial 3-oxoacyl-CoA thiolase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.2
Summary	mitochondrial matrix protein with acetyl-Coenzyme A acyltransferase 2 activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130433.1	NP_569117.1	3-ketoacyl-CoA thiolase, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACAA2	NP_006102.2
Bos taurus (cattle)	ACAA2	NP_001030419.1
Rattus norvegicus (Norway rat)	Acaa2	NP_569117.1
Pan troglodytes (chimpanzee)	ACAA2	XP_512127.3
Danio rerio (zebrafish)	acaa2	NP_998217.1
Mus musculus (house mouse)	Acaa2	NP_803421.1
Gallus gallus (chicken)	ACAA2	NP_001006571.1
Caenorhabditis elegans (roundworm)	F53A2.7	NP_499752.2
Xenopus tropicalis (tropical clawed frog)	acaa2	NP_001072185.1
	ACAA2	XP_001097873.2
Drosophila melanogaster (fruit fly)	yip2	NP_523528.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006821	XP_308924.2

Gene Ontology
Bibliography

Related articles in PubMed

The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities.
Kiema TR, Harijan RK, Strozyk M, Fukao T, Alexson SE, Wierenga RK
Acta crystallographica. Section D, Biological crystallography70(Pt 12)3212-25(2014 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Acetyl-Coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines.
Cao W, Liu N, Tang S, Bao L, Shen L, Yuan H, Zhao X, Lu H
Biochimica et biophysica acta1780(6)873-80(2008 Jun)

Acetate generation in rat liver mitochondria; acetyl-CoA hydrolase activity is demonstrated by 3-ketoacyl-CoA thiolase.
Yamashita H, Itsuki A, Kimoto M, Hiemori M, Tsuji H
Biochimica et biophysica acta1761(1)17-23(2006 Jan)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Acaa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acaa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12446	NM_130433.1 Latest version!	Rattus norvegicus acetyl-CoA acyltransferase 2 (Acaa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acaa2 ( NM_130433.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_569117.1 Rattus norvegicus acetyl-CoA acyltransferase 2 (Acaa2), mRNA.

Price & Availability↑

CloneID	ORa12446
Clone ID Related Accession (Same CDS sequence)	NM_130433.1
Accession Version	NM_130433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	3-ketoacyl-CoA thiolase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X05341.1. ##Evidence-Data-START## Transcript exon combination :: X05341.1, FQ228855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCTGC TACGAGGTGT GTTTATCGTT GCTGCGAAGC GAACACCCTT TGGAGCTTAT 
GGGGGTCTTC TCAAGGACTT CACTGCCACT GACTTAACTG AATTTGCTGC CAGGGCTGCC 
CTGTCTGCTG GCAAAGTTCC ACCGGAAACC ATCGATAGTG TCATCGTGGG CAATGTCATG 
CAGAGCTCTT CAGATGCGGC GTACCTGGCA AGGCATGTGG GTTTACGTGT GGGAGTCCCG 
ACGGAGACTG GGGCCCTCAC CCTCAACAGA CTCTGTGGCT CTGGTTTCCA GTCCATCGTG 
AGCGGATGTC AGGAAATCTG CTCGAAAGAC GCTGAGGTCG TCTTATGTGG AGGAACCGAG 
AGCATGAGCC AGTCCCCCTA CTCTGTCAGA AATGTGCGCT TCGGAACCAA ATTTGGGTTA 
GATCTCAAGC TGGAAGATAC TTTGTGGGCA GGATTAACGG ATCAACACGT GAAGCTCCCC 
ATGGGGATGA CTGCAGAGAA CCTGGCTGCA AAATACAACA TAAGCAGAGA AGACTGCGAC 
AGATACGCCC TGCAGTCCCA GCAGAGGTGG AAAGCCGCTA ACGAGGCTGG CTACTTTAAT 
GAGGAGATGG CCCCCATTGA GGTGAAGACC AAGAAGGGCA AACAGACCAT GCAAGTGGAT 
GAGCACGCCC GGCCCCAAAC GACCCTGGAG CAGCTGCAGA ACCTCCCGCC AGTGTTCAAG 
AAAGAGGGGA CGGTCACAGC AGGGAACGCC TCGGGCATGT CTGACGGTGC TGGGGTCGTC 
ATCATAGCCA GCGAAGATGC TGTCAAAAAA CATAACTTCA CACCACTGGC CAGAGTCGTG 
GGCTACTTTG TGTCTGGATG TGACCCTGCT ATCATGGGGA TCGGTCCAGT CCCTGCCATC 
ACTGGAGCAT TGAAGAAAGC TGGGCTGAGC CTTAAGGACA TGGATTTGAT AGACGTGAAT 
GAAGCATTTG CTCCTCAGTT CTTGGCTGTT CAGAAGAGCT TGGATCTCGA CCCCAGTAAA 
ACCAACGTGA GTGGAGGTGC CATAGCCCTG GGTCACCCGC TGGGAGGATC TGGATCCAGA 
ATCACCGCAC ACCTGGTTCA CGAGTTAAGG CGTCGAGGTG GAAAATACGC AGTGGGATCA 
GCTTGCATTG GAGGTGGCCA AGGCATCTCC CTGATCATCC AGAACACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569117.1
CDS	165..1358
Translation	MALLRGVFIVAAKRTPFGAYGGLLKDFTATDLTEFAARAALSAGKVPPETIDSVIVGNVMQSSSDAAYLARHVGLRVGVPTETGALTLNRLCGSGFQSIVSGCQEICSKDAEVVLCGGTESMSQSPYSVRNVRFGTKFGLDLKLEDTLWAGLTDQHVKLPMGMTAENLAAKYNISREDCDRYALQSQQRWKAANEAGYFNEEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQNLPPVFKKEGTVTAGNASGMSDGAGVVIIASEDAVKKHNFTPLARVVGYFVSGCDPAIMGIGPVPAITGALKKAGLSLKDMDLIDVNEAFAPQFLAVQKSLDLDPSKTNVSGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGISLIIQNTA

Target ORF information:

RefSeq Version	NM_130433.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acetyl-CoA acyltransferase 2 (Acaa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130433.1

ORF Insert Sequence:

ATGGCGCTGC TACGAGGTGT GTTTATCGTT GCTGCGAAGC GAACACCCTT TGGAGCTTAT 
GGGGGTCTTC TCAAGGACTT CACTGCCACT GACTTAACTG AATTTGCTGC CAGGGCTGCC 
CTGTCTGCTG GCAAAGTTCC ACCGGAAACC ATCGATAGTG TCATCGTGGG CAATGTCATG 
CAGAGCTCTT CAGATGCGGC GTACCTGGCA AGGCATGTGG GTTTACGTGT GGGAGTCCCG 
ACGGAGACTG GGGCCCTCAC CCTCAACAGA CTCTGTGGCT CTGGTTTCCA GTCCATCGTG 
AGCGGATGTC AGGAAATCTG CTCGAAAGAC GCTGAGGTCG TCTTATGTGG AGGAACCGAG 
AGCATGAGCC AGTCCCCCTA CTCTGTCAGA AATGTGCGCT TCGGAACCAA ATTTGGGTTA 
GATCTCAAGC TGGAAGATAC TTTGTGGGCA GGATTAACGG ATCAACACGT GAAGCTCCCC 
ATGGGGATGA CTGCAGAGAA CCTGGCTGCA AAATACAACA TAAGCAGAGA AGACTGCGAC 
AGATACGCCC TGCAGTCCCA GCAGAGGTGG AAAGCCGCTA ACGAGGCTGG CTACTTTAAT 
GAGGAGATGG CCCCCATTGA GGTGAAGACC AAGAAGGGCA AACAGACCAT GCAAGTGGAT 
GAGCACGCCC GGCCCCAAAC GACCCTGGAG CAGCTGCAGA ACCTCCCGCC AGTGTTCAAG 
AAAGAGGGGA CGGTCACAGC AGGGAACGCC TCGGGCATGT CTGACGGTGC TGGGGTCGTC 
ATCATAGCCA GCGAAGATGC TGTCAAAAAA CATAACTTCA CACCACTGGC CAGAGTCGTG 
GGCTACTTTG TGTCTGGATG TGACCCTGCT ATCATGGGGA TCGGTCCAGT CCCTGCCATC 
ACTGGAGCAT TGAAGAAAGC TGGGCTGAGC CTTAAGGACA TGGATTTGAT AGACGTGAAT 
GAAGCATTTG CTCCTCAGTT CTTGGCTGTT CAGAAGAGCT TGGATCTCGA CCCCAGTAAA 
ACCAACGTGA GTGGAGGTGC CATAGCCCTG GGTCACCCGC TGGGAGGATC TGGATCCAGA 
ATCACCGCAC ACCTGGTTCA CGAGTTAAGG CGTCGAGGTG GAAAATACGC AGTGGGATCA 
GCTTGCATTG GAGGTGGCCA AGGCATCTCC CTGATCATCC AGAACACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities.
Acta crystallographica. Section D, Biological crystallography70(Pt 12)3212-25(2014 Dec)
Kiema TR,Harijan RK,Strozyk M,Fukao T,Alexson SE,Wierenga RK

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Acetyl-Coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines.
Biochimica et biophysica acta1780(6)873-80(2008 Jun)
Cao W,Liu N,Tang S,Bao L,Shen L,Yuan H,Zhao X,Lu H

Acetate generation in rat liver mitochondria; acetyl-CoA hydrolase activity is demonstrated by 3-ketoacyl-CoA thiolase.
Biochimica et biophysica acta1761(1)17-23(2006 Jan)
Yamashita H,Itsuki A,Kimoto M,Hiemori M,Tsuji H

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Acaa2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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