Slc28a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc28a3
Entrez Gene ID	140944
Full Name	solute carrier family 28 member 3
Synonyms	Cnt3
General protein information	Preferred Names solute carrier family 28 member 3 Names solute carrier family 28 member 3 CNT 3 concentrative Na(+)-nucleoside cotransporter 3 rCNT3 solute carrier family 28 (concentrative nucleoside transporter), member 3 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	member of a family of sodium-dependent, concentrative nucleoside transporters; may transport both pyrimidine and purine nucleosides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008771422.2	XP_008769644.1	solute carrier family 28 member 3 isoform X1
NM_080908.1	NP_543184.1	solute carrier family 28 member 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC28A3	XP_528339.2
	SLC28A3	XP_001106570.1
Caenorhabditis elegans (roundworm)	F27E11.1	NP_503967.1
Gallus gallus (chicken)	SLC28A3	XP_004949268.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005989	XP_556667.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005988	XP_316031.4
Canis lupus familiaris (dog)	SLC28A3	XP_005615872.1
Rattus norvegicus (Norway rat)	Slc28a3	NP_543184.1
Neurospora crassa	NCU08148	XP_959559.1
Homo sapiens (human)	SLC28A3	NP_071410.1
Mus musculus (house mouse)	Slc28a3	NP_071712.3
Caenorhabditis elegans (roundworm)	F27E11.2	NP_503966.2
Magnaporthe oryzae (rice blast fungus)	MGG_05467	XP_003710333.1
Xenopus tropicalis (tropical clawed frog)	slc28a3	XP_002935023.2
Bos taurus (cattle)	SLC28A3	NP_001179096.1
Drosophila melanogaster (fruit fly)	CG8083	NP_610446.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional crosstalk between the adenosine transporter CNT3 and purinergic receptors in the biliary epithelia.
Godoy V, Banales JM, Medina JF, Pastor-Anglada M
Journal of hepatology61(6)1337-43(2014 Dec)

Functional and molecular characterization of adenosine transport at the rat inner blood-retinal barrier.
Nagase K, Tomi M, Tachikawa M, Hosoya K
Biochimica et biophysica acta1758(1)13-9(2006 Jan)

Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
Rodr?guez-Mulero S, Errasti-Murugarren E, Ballar?n J, Felipe A, Doucet A, Casado FJ, Pastor-Anglada M
Kidney international68(2)665-72(2005 Aug)

The concentrative nucleoside transporter family, SLC28.
Gray JH, Owen RP, Giacomini KM
Pflugers Archiv : European journal of physiology447(5)728-34(2004 Feb)

Molecular identification and characterization of novel human and mouse concentrative Na+-nucleoside cotransporter proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib).
Ritzel MW, Ng AM, Yao SY, Graham K, Loewen SK, Smith KM, Ritzel RG, Mowles DA, Carpenter P, Chen XZ, Karpinski E, Hyde RJ, Baldwin SA, Cass CE, Young JD
The Journal of biological chemistry276(4)2914-27(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There is functional crosstalk between the adenosine transporter CNT3 and purinergic receptors in the cholangiocytes in rats.
Title: Functional crosstalk between the adenosine transporter CNT3 and purinergic receptors in the biliary epithelia.

Diabetes increased CNT1 and CNT3 expression in the glomerulus and insulin treatment decreased it.
Title: Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.

The following Slc28a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc28a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00302	NM_080908.1 Latest version!	Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa44196	XM_008771422.2 Latest version!	Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc28a3 ( NM_080908.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_543184.1 Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), mRNA.

Price & Availability↑

CloneID	ORa00302
Clone ID Related Accession (Same CDS sequence)	NM_080908.1
Accession Version	NM_080908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-09
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 28 member 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY059414.1. ##Evidence-Data-START## Transcript exon combination :: AY059414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAGGT CAGACCCAGA CCCGGGAAAG AATTCGGAGC CCAGTAAGAG CAAGATGAGC 
CTGGAGCTGA GGCCAACAGC CCCCAGCGAC CAGGGCCGCC TCAACGAAGC CTTCCAGGAT 
GAAGACCTGG AAGAGCAGAA CGCTCCAGGA AACAGCACAG TCAGGAGCAG AGTTGTACAG 
AGTGGGGAGC AAGGACGGGC CAAACAGGAC GACAGGCAGA TCACGATTGA GCAGGAGCCT 
CTAGGACCCA AAGAAGGCAC AGAGGAAGAG AGCGAAGATG AACGTCAGAA AGGGTTCTTG 
GAAAGGAAGT ATGATACGGT TTGTGAGTTT TGTAGAAAGC ACAGAGTCAT TCTCCAGCAC 
ACCATCTGGG CAGTTTTACT GACAGGTTTC CTGGCCCTGG TGATTGCAGC CTGTGCACTA 
AACTTCCACC GAGCCCTTCC CCTTTTTGTG ATCACCTTGG TGACCATCTT CTTCGTCGTC 
TGGGATCGCT TGATGGCCAA ATACGAACAG CGAATCGATG ATGTCCTGTC TCCCGGCAAA 
AGGCTGCTTG AGAGGCATTG GTTCTGGCTG AAGTGGGTGG TCTGGTGCTC ACTGATCTTG 
GCGGTCATTC TCTGGCTGGC TCTGGACACG GCCAGGCTGG GCCAACAGCA GCTGATATCC 
TTTGGTGGGC TCGTCATGTA TATTGTTCTG CTATTCCTGT TTTCCAAGCA TCCGACCAGA 
GTTTACTGGA GGCCTGTCTT TTGGGGAATT GGACTGCAGT TTCTTCTTGG GCTCTTAATT 
CTAAGGACTA GGCCTGGATT TGTTGCTTTT GATTGGATGG GGAAACAAGT TCAGACTTTT 
CTGGGGTACA CTGACGCTGG AGCCCAGTTT GTCTTTGGAG AAAAGTATAC AGACCACTTC 
TTTGCATTTA AGATCCTTCC CATTGTGGTT TTCTTCAGCA CCGTGATGTC TATGCTCTAT 
TATCTGGGAC TGATGCAGTG GATTATCAGA AAGGTTGGGT GGCTAATGCT TGTCACTATG 
GGGTCATCTC CCATTGAGTC CGTAGTTGCT GCTGGCAACA TATTTGTTGG GCAAACAGAG 
TCTCCACTAC TGGTTCAACC ATATCTACCA CACGTCACCA AGTCAGAACT GCACACCATC 
ATGACAGCTG GTTTTGCCAC GATTGCTGGG AGCGTGTTGG GCGCGTACAT CTCTTTTGGG 
GTGTCATCTA CACACTTGCT CACCGCTTCT GTCATGTCGG CGCCTGCAGC CCTGGCTGTG 
GCCAAACTCT TTTGGCCTGA AACAGAGAAA CCCAAAATAA CCCTCAAGAA TGCCATGAAA 
ATGGAAAATG GTGATTCCCG GAATCTCCTG GAGGCGGCCA CGCAGGGTGC ATCTTCGTCC 
ATCCCCCTGG TGGCAAACAT CGCTGCAAAT CTGATCGCCT TCCTGGCCTT GCTTTCCTTT 
GTGAACTCCG CTCTGTCTTG GTTTGGAAGC ATGTTCGACT ACCCACAGCT GAGTTTCGAG 
CTCATTTGTT CTTACATCTT CATGCCTTTC TCCTTCATGA TGGGAGTCGA CTGGCAAGAC 
AGATTTATGG TCGCCAAACT CATAGGCTAC AAGACGTTCT TCAATGAATT TGTGGCTTAT 
GAACACCTCT CAAAATTCAT CAATTTGAGG AAAGCGGCTG GACCCAAATT TGTGAATGGT 
GTGCAGCAAT ATATGTCAAT TCGCTCGGAG ACCATCGCAA CTTACGCCCT CTGTGGTTTT 
GCCAATTTTG GTTCCCTAGG AATAGTGATC GGTGGACTTA CGTCTATAGC TCCGTCCAGA 
AAGCGAGACA TTGCCTCGGG AGCCATGAGA GCCCTGATTG CTGGGACCAT TGCCTGCTTC 
ATGACAGCCT GCATCGCTGG CATGCTCTCT GACACCCCGG TGGCTATCAA CTGCCACCAC 
GTTCTAGAGA GCAGCAAAGT ACTCAGCAAC ACAACTGAAG TGGCGTCTTG CTGTCAAGGC 
CTGTTCAACA GCACTGTTGC CAGGGGACCA AATGACGTCC TCCCAGGAGG AAACTTCAGC 
CTCTACACTT TGAAGAGCTG CTGCAACCTG CTGAAACCAC CAACGCTGAA CTGTGGTTGG 
ATCCCTAACA TACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543184.1
CDS	1..2118
Translation	MSRSDPDPGKNSEPSKSKMSLELRPTAPSDQGRLNEAFQDEDLEEQNAPGNSTVRSRVVQSGEQGRAKQDDRQITIEQEPLGPKEGTEEESEDERQKGFLERKYDTVCEFCRKHRVILQHTIWAVLLTGFLALVIAACALNFHRALPLFVITLVTIFFVVWDRLMAKYEQRIDDVLSPGKRLLERHWFWLKWVVWCSLILAVILWLALDTARLGQQQLISFGGLVMYIVLLFLFSKHPTRVYWRPVFWGIGLQFLLGLLILRTRPGFVAFDWMGKQVQTFLGYTDAGAQFVFGEKYTDHFFAFKILPIVVFFSTVMSMLYYLGLMQWIIRKVGWLMLVTMGSSPIESVVAAGNIFVGQTESPLLVQPYLPHVTKSELHTIMTAGFATIAGSVLGAYISFGVSSTHLLTASVMSAPAALAVAKLFWPETEKPKITLKNAMKMENGDSRNLLEAATQGASSSIPLVANIAANLIAFLALLSFVNSALSWFGSMFDYPQLSFELICSYIFMPFSFMMGVDWQDRFMVAKLIGYKTFFNEFVAYEHLSKFINLRKAAGPKFVNGVQQYMSIRSETIATYALCGFANFGSLGIVIGGLTSIAPSRKRDIASGAMRALIAGTIACFMTACIAGMLSDTPVAINCHHVLESSKVLSNTTEVASCCQGLFNSTVARGPNDVLPGGNFSLYTLKSCCNLLKPPTLNCGWIPNIP

Target ORF information:

RefSeq Version	NM_080908.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080908.1

ORF Insert Sequence:

ATGTCCAGGT CAGACCCAGA CCCGGGAAAG AATTCGGAGC CCAGTAAGAG CAAGATGAGC 
CTGGAGCTGA GGCCAACAGC CCCCAGCGAC CAGGGCCGCC TCAACGAAGC CTTCCAGGAT 
GAAGACCTGG AAGAGCAGAA CGCTCCAGGA AACAGCACAG TCAGGAGCAG AGTTGTACAG 
AGTGGGGAGC AAGGACGGGC CAAACAGGAC GACAGGCAGA TCACGATTGA GCAGGAGCCT 
CTAGGACCCA AAGAAGGCAC AGAGGAAGAG AGCGAAGATG AACGTCAGAA AGGGTTCTTG 
GAAAGGAAGT ATGATACGGT TTGTGAGTTT TGTAGAAAGC ACAGAGTCAT TCTCCAGCAC 
ACCATCTGGG CAGTTTTACT GACAGGTTTC CTGGCCCTGG TGATTGCAGC CTGTGCACTA 
AACTTCCACC GAGCCCTTCC CCTTTTTGTG ATCACCTTGG TGACCATCTT CTTCGTCGTC 
TGGGATCGCT TGATGGCCAA ATACGAACAG CGAATCGATG ATGTCCTGTC TCCCGGCAAA 
AGGCTGCTTG AGAGGCATTG GTTCTGGCTG AAGTGGGTGG TCTGGTGCTC ACTGATCTTG 
GCGGTCATTC TCTGGCTGGC TCTGGACACG GCCAGGCTGG GCCAACAGCA GCTGATATCC 
TTTGGTGGGC TCGTCATGTA TATTGTTCTG CTATTCCTGT TTTCCAAGCA TCCGACCAGA 
GTTTACTGGA GGCCTGTCTT TTGGGGAATT GGACTGCAGT TTCTTCTTGG GCTCTTAATT 
CTAAGGACTA GGCCTGGATT TGTTGCTTTT GATTGGATGG GGAAACAAGT TCAGACTTTT 
CTGGGGTACA CTGACGCTGG AGCCCAGTTT GTCTTTGGAG AAAAGTATAC AGACCACTTC 
TTTGCATTTA AGATCCTTCC CATTGTGGTT TTCTTCAGCA CCGTGATGTC TATGCTCTAT 
TATCTGGGAC TGATGCAGTG GATTATCAGA AAGGTTGGGT GGCTAATGCT TGTCACTATG 
GGGTCATCTC CCATTGAGTC CGTAGTTGCT GCTGGCAACA TATTTGTTGG GCAAACAGAG 
TCTCCACTAC TGGTTCAACC ATATCTACCA CACGTCACCA AGTCAGAACT GCACACCATC 
ATGACAGCTG GTTTTGCCAC GATTGCTGGG AGCGTGTTGG GCGCGTACAT CTCTTTTGGG 
GTGTCATCTA CACACTTGCT CACCGCTTCT GTCATGTCGG CGCCTGCAGC CCTGGCTGTG 
GCCAAACTCT TTTGGCCTGA AACAGAGAAA CCCAAAATAA CCCTCAAGAA TGCCATGAAA 
ATGGAAAATG GTGATTCCCG GAATCTCCTG GAGGCGGCCA CGCAGGGTGC ATCTTCGTCC 
ATCCCCCTGG TGGCAAACAT CGCTGCAAAT CTGATCGCCT TCCTGGCCTT GCTTTCCTTT 
GTGAACTCCG CTCTGTCTTG GTTTGGAAGC ATGTTCGACT ACCCACAGCT GAGTTTCGAG 
CTCATTTGTT CTTACATCTT CATGCCTTTC TCCTTCATGA TGGGAGTCGA CTGGCAAGAC 
AGATTTATGG TCGCCAAACT CATAGGCTAC AAGACGTTCT TCAATGAATT TGTGGCTTAT 
GAACACCTCT CAAAATTCAT CAATTTGAGG AAAGCGGCTG GACCCAAATT TGTGAATGGT 
GTGCAGCAAT ATATGTCAAT TCGCTCGGAG ACCATCGCAA CTTACGCCCT CTGTGGTTTT 
GCCAATTTTG GTTCCCTAGG AATAGTGATC GGTGGACTTA CGTCTATAGC TCCGTCCAGA 
AAGCGAGACA TTGCCTCGGG AGCCATGAGA GCCCTGATTG CTGGGACCAT TGCCTGCTTC 
ATGACAGCCT GCATCGCTGG CATGCTCTCT GACACCCCGG TGGCTATCAA CTGCCACCAC 
GTTCTAGAGA GCAGCAAAGT ACTCAGCAAC ACAACTGAAG TGGCGTCTTG CTGTCAAGGC 
CTGTTCAACA GCACTGTTGC CAGGGGACCA AATGACGTCC TCCCAGGAGG AAACTTCAGC 
CTCTACACTT TGAAGAGCTG CTGCAACCTG CTGAAACCAC CAACGCTGAA CTGTGGTTGG 
ATCCCTAACA TACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc28a3 ( XM_008771422.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769644.1 Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44196
Clone ID Related Accession (Same CDS sequence)	XM_008771422.2
Accession Version	XM_008771422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 28 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGT CAGACCCAGA CCCGGGAAAG AATTCGGAGC CCAGTAAGAG CAAGATGAGC 
CTGGAGCTGA GGCCAACAGC CCCCAGCGAC CAGGGCCGCC TCAACGAAGC CTTCCAGGAT 
GAAGACCTGG AAGAGCAGAA CGCTCCAGGA AACAGCACAG TCAGGAGCAG AGTTGTACAG 
AGTGGGGAGC AAGGACGGGC CAAACAGGAC GACAGGCAGA TCACGATTGA GCAGGAGCCT 
CTAGGACCCA AAGAAGGCAC AGAGGAAGAG AGCGAAGATG AACGTCAGAA AGGGTTCTTG 
GAAAGGAAGT ATGATACGGT TTGTGAGTTT TGTAGAAAGC ACAGAGTCAT TCTCCAGCAC 
ACCATCTGGG CAGTTTTACT GACAGGTTTC CTGGCCCTGG TGATTGCAGC CTGTGCACTA 
AACTTCCACC GAGCCCTTCC CCTTTTTGTG ATCACCTTGG TGACCATCTT CTTCGTCGTC 
TGGGATCGCT TGATGGCCAA ATACGAACAG CGAATCGATG ATGTCCTGTC TCCCGGCAAA 
AGGCTGCTTG AGAGGCATTG GTTCTGGCTG AAGTGGGTGG TCTGGTGCTC ACTGATCTTG 
GCGGTCATTC TCTGGCTGGC TCTGGACACG GCCAGGCTGG GCCAACAGCA GCTGATATCC 
TTTGGTGGGC TCGTCATGTA TATTGTTCTG CTATTCCTGT TTTCCAAGCA TCCGACCAGA 
GTTTACTGGA GGCCTGTCTT TTGGGGAATT GGACTGCAGT TTCTTCTTGG GCTCTTAATT 
CTAAGGACTA GGCCTGGATT TGTTGCTTTT GATTGGATGG GGAAACAAGT TCAGACTTTT 
CTGGGGTACA CTGACGCTGG AGCCCAGTTT GTCTTTGGAG AAAAGTATAC AGACCACTTC 
TTTGCATTTA AGATCCTTCC CATTGTGGTT TTCTTCAGCA CCGTGATGTC TATGCTCTAT 
TATCTGGGAC TGATGCAGTG GATTATCAGA AAGGTTGGGT GGCTAATGCT TGTCACTATG 
GGGTCATCTC CCATTGAGTC CGTAGTTGCT GCTGGCAACA TATTTGTTGG GCAAACAGAG 
TCTCCACTAC TGGTTCAACC ATATCTACCA CACGTCACCA AGTCAGAACT GCACACCATC 
ATGACAGCTG GTTTTGCCAC GATTGCTGGG AGCGTGTTGG GCGCGTACAT CTCTTTTGGG 
GTGTCATCTA CACACTTGCT CACCGCTTCT GTCATGTCGG CGCCTGCAGC CCTGGCTGTG 
GCCAAACTCT TTTGGCCTGA AACAGAGAAA CCCAAAATAA CCCTCAAGAA TGCCATGAAA 
ATGGAAAATG GTGATTCCCG GAATCTCCTG GAGGCGGCCA CGCAGGGTGC ATCTTCGTCC 
ATCCCCCTGG TGGCAAACAT CGCTGCAAAT CTGATCGCCT TCCTGGCCTT GCTTTCCTTT 
GTGAACTCCG CTCTGTCTTG GTTTGGAAGC ATGTTCGACT ACCCACAGCT GAGTTTCGAG 
CTCATTTGTT CTTACATCTT CATGCCTTTC TCCTTCATGA TGGGAGTCGA CTGGCAAGAC 
AGCTTTATGG TCGCCAAACT CATAGGCTAC AAGACGTTCT TCAATGAATT TGTGGCTTAT 
GAACACCTCT CAAAATTCAT CAATTTGAGG AAAGCGGCTG GACCCAAATT TGTGAATGGT 
GTGCAGCAAT ATATGTCAAT TCGCTCGGAG ACCATCGCAA CTTACGCCCT CTGTGGTTTT 
GCCAATTTCG GTTCCCTAGG AATAGTGATC GGTGGACTTA CGTCCATAGC TCCGTCCAGA 
AAGCGAGACA TTGCCTCGGG AGCCATGAGA GCCCTGATTG CTGGGACCAT TGCCTGCTTC 
ATGACAGCCT GCATCGCTGG CATGCTCTCT GACACCCCGG TGGCTATCAA CTGCCACCAC 
GTTCTAGAGA GCAGCAAAGT ACTCAGCAAC ACAACTGAAG TGGCGTCTTG CTGTCAAGAC 
CTGTTCAACA GCACTGTTGC CAGGGGACCA AATGACGTCC TCCCAGGAGG AAACTTCAGC 
CTCTACACTT TGAAGAGCTG CTGCAACCTG CTGAAACCAC CAACGCTGAA CTGTGGTTGG 
ATCCCTAACA TACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769644.1
CDS	86..2203
Translation	MSRSDPDPGKNSEPSKSKMSLELRPTAPSDQGRLNEAFQDEDLEEQNAPGNSTVRSRVVQSGEQGRAKQDDRQITIEQEPLGPKEGTEEESEDERQKGFLERKYDTVCEFCRKHRVILQHTIWAVLLTGFLALVIAACALNFHRALPLFVITLVTIFFVVWDRLMAKYEQRIDDVLSPGKRLLERHWFWLKWVVWCSLILAVILWLALDTARLGQQQLISFGGLVMYIVLLFLFSKHPTRVYWRPVFWGIGLQFLLGLLILRTRPGFVAFDWMGKQVQTFLGYTDAGAQFVFGEKYTDHFFAFKILPIVVFFSTVMSMLYYLGLMQWIIRKVGWLMLVTMGSSPIESVVAAGNIFVGQTESPLLVQPYLPHVTKSELHTIMTAGFATIAGSVLGAYISFGVSSTHLLTASVMSAPAALAVAKLFWPETEKPKITLKNAMKMENGDSRNLLEAATQGASSSIPLVANIAANLIAFLALLSFVNSALSWFGSMFDYPQLSFELICSYIFMPFSFMMGVDWQDSFMVAKLIGYKTFFNEFVAYEHLSKFINLRKAAGPKFVNGVQQYMSIRSETIATYALCGFANFGSLGIVIGGLTSIAPSRKRDIASGAMRALIAGTIACFMTACIAGMLSDTPVAINCHHVLESSKVLSNTTEVASCCQDLFNSTVARGPNDVLPGGNFSLYTLKSCCNLLKPPTLNCGWIPNIP

Target ORF information:

RefSeq Version	XM_008771422.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 3 (Slc28a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771422.2

ORF Insert Sequence:

ATGTCCAGGT CAGACCCAGA CCCGGGAAAG AATTCGGAGC CCAGTAAGAG CAAGATGAGC 
CTGGAGCTGA GGCCAACAGC CCCCAGCGAC CAGGGCCGCC TCAACGAAGC CTTCCAGGAT 
GAAGACCTGG AAGAGCAGAA CGCTCCAGGA AACAGCACAG TCAGGAGCAG AGTTGTACAG 
AGTGGGGAGC AAGGACGGGC CAAACAGGAC GACAGGCAGA TCACGATTGA GCAGGAGCCT 
CTAGGACCCA AAGAAGGCAC AGAGGAAGAG AGCGAAGATG AACGTCAGAA AGGGTTCTTG 
GAAAGGAAGT ATGATACGGT TTGTGAGTTT TGTAGAAAGC ACAGAGTCAT TCTCCAGCAC 
ACCATCTGGG CAGTTTTACT GACAGGTTTC CTGGCCCTGG TGATTGCAGC CTGTGCACTA 
AACTTCCACC GAGCCCTTCC CCTTTTTGTG ATCACCTTGG TGACCATCTT CTTCGTCGTC 
TGGGATCGCT TGATGGCCAA ATACGAACAG CGAATCGATG ATGTCCTGTC TCCCGGCAAA 
AGGCTGCTTG AGAGGCATTG GTTCTGGCTG AAGTGGGTGG TCTGGTGCTC ACTGATCTTG 
GCGGTCATTC TCTGGCTGGC TCTGGACACG GCCAGGCTGG GCCAACAGCA GCTGATATCC 
TTTGGTGGGC TCGTCATGTA TATTGTTCTG CTATTCCTGT TTTCCAAGCA TCCGACCAGA 
GTTTACTGGA GGCCTGTCTT TTGGGGAATT GGACTGCAGT TTCTTCTTGG GCTCTTAATT 
CTAAGGACTA GGCCTGGATT TGTTGCTTTT GATTGGATGG GGAAACAAGT TCAGACTTTT 
CTGGGGTACA CTGACGCTGG AGCCCAGTTT GTCTTTGGAG AAAAGTATAC AGACCACTTC 
TTTGCATTTA AGATCCTTCC CATTGTGGTT TTCTTCAGCA CCGTGATGTC TATGCTCTAT 
TATCTGGGAC TGATGCAGTG GATTATCAGA AAGGTTGGGT GGCTAATGCT TGTCACTATG 
GGGTCATCTC CCATTGAGTC CGTAGTTGCT GCTGGCAACA TATTTGTTGG GCAAACAGAG 
TCTCCACTAC TGGTTCAACC ATATCTACCA CACGTCACCA AGTCAGAACT GCACACCATC 
ATGACAGCTG GTTTTGCCAC GATTGCTGGG AGCGTGTTGG GCGCGTACAT CTCTTTTGGG 
GTGTCATCTA CACACTTGCT CACCGCTTCT GTCATGTCGG CGCCTGCAGC CCTGGCTGTG 
GCCAAACTCT TTTGGCCTGA AACAGAGAAA CCCAAAATAA CCCTCAAGAA TGCCATGAAA 
ATGGAAAATG GTGATTCCCG GAATCTCCTG GAGGCGGCCA CGCAGGGTGC ATCTTCGTCC 
ATCCCCCTGG TGGCAAACAT CGCTGCAAAT CTGATCGCCT TCCTGGCCTT GCTTTCCTTT 
GTGAACTCCG CTCTGTCTTG GTTTGGAAGC ATGTTCGACT ACCCACAGCT GAGTTTCGAG 
CTCATTTGTT CTTACATCTT CATGCCTTTC TCCTTCATGA TGGGAGTCGA CTGGCAAGAC 
AGCTTTATGG TCGCCAAACT CATAGGCTAC AAGACGTTCT TCAATGAATT TGTGGCTTAT 
GAACACCTCT CAAAATTCAT CAATTTGAGG AAAGCGGCTG GACCCAAATT TGTGAATGGT 
GTGCAGCAAT ATATGTCAAT TCGCTCGGAG ACCATCGCAA CTTACGCCCT CTGTGGTTTT 
GCCAATTTCG GTTCCCTAGG AATAGTGATC GGTGGACTTA CGTCCATAGC TCCGTCCAGA 
AAGCGAGACA TTGCCTCGGG AGCCATGAGA GCCCTGATTG CTGGGACCAT TGCCTGCTTC 
ATGACAGCCT GCATCGCTGG CATGCTCTCT GACACCCCGG TGGCTATCAA CTGCCACCAC 
GTTCTAGAGA GCAGCAAAGT ACTCAGCAAC ACAACTGAAG TGGCGTCTTG CTGTCAAGAC 
CTGTTCAACA GCACTGTTGC CAGGGGACCA AATGACGTCC TCCCAGGAGG AAACTTCAGC 
CTCTACACTT TGAAGAGCTG CTGCAACCTG CTGAAACCAC CAACGCTGAA CTGTGGTTGG 
ATCCCTAACA TACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional crosstalk between the adenosine transporter CNT3 and purinergic receptors in the biliary epithelia.
Journal of hepatology61(6)1337-43(2014 Dec)
Godoy V,Banales JM,Medina JF,Pastor-Anglada M

Functional and molecular characterization of adenosine transport at the rat inner blood-retinal barrier.
Biochimica et biophysica acta1758(1)13-9(2006 Jan)
Nagase K,Tomi M,Tachikawa M,Hosoya K

Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
Kidney international68(2)665-72(2005 Aug)
Rodr?guez-Mulero S,Errasti-Murugarren E,Ballar?n J,Felipe A,Doucet A,Casado FJ,Pastor-Anglada M

The concentrative nucleoside transporter family, SLC28.
Pflugers Archiv : European journal of physiology447(5)728-34(2004 Feb)
Gray JH,Owen RP,Giacomini KM

Molecular identification and characterization of novel human and mouse concentrative Na+-nucleoside cotransporter proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib).
The Journal of biological chemistry276(4)2914-27(2001 Jan)
Ritzel MW,Ng AM,Yao SY,Graham K,Loewen SK,Smith KM,Ritzel RG,Mowles DA,Carpenter P,Chen XZ,Karpinski E,Hyde RJ,Baldwin SA,Cass CE,Young JD