Ddit4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddit4
Entrez Gene ID	140942
Full Name	DNA-damage-inducible transcript 4
Synonyms	Rtp801
General protein information	Preferred Names DNA-damage-inducible transcript 4 Names DNA damage-inducible transcript 4 protein HIF-1 responsive RTP801 HIF-1 responsive protein RTP801 REDD-1 protein regulated in development and DNA damage response 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	HIF-1-responsive gene that may protect some types of cells from hypoxia and H(2)O(2)-triggered apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080906.2	NP_543182.1	DNA damage-inducible transcript 4 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDIT4	NP_061931.1
Rattus norvegicus (Norway rat)	Ddit4	NP_543182.1
	DDIT4	XP_002805733.1
Xenopus tropicalis (tropical clawed frog)	ddit4	NP_989175.1
Pan troglodytes (chimpanzee)	DDIT4	XP_003951715.1
Danio rerio (zebrafish)	ddit4	NP_956401.1
Canis lupus familiaris (dog)	DDIT4	XP_546156.2
Bos taurus (cattle)	DDIT4	NP_001069390.1
Mus musculus (house mouse)	Ddit4	NP_083359.1

Gene Ontology
Bibliography

Related articles in PubMed

Time-Course Change of Redd1 Expressions in the Hippocampal CA1 Region Following Chronic Cerebral Hypoperfusion.
Park JA, Lee CH
Cellular and molecular neurobiology37(3)563-569(2017 Apr)

RTP801 Is Involved in Mutant Huntingtin-Induced Cell Death.
Mart?n-Flores N, Roman?-Aumedes J, Ru? L, Canal M, Sanders P, Straccia M, Allen ND, Alberch J, Canals JM, P?rez-Navarro E, Malagelada C
Molecular neurobiology53(5)2857-2868(2016 07)

Up-regulation of miRNA-221 inhibits hypoxia/reoxygenation-induced autophagy through the DDIT4/mTORC1 and Tp53inp1/p62 pathways.
Chen Q, Zhou Y, Richards AM, Wang P
Biochemical and biophysical research communications474(1)168-174(2016 05)

REDD1 attenuates cardiac hypertrophy via enhancing autophagy.
Liu C, Xue R, Wu D, Wu L, Chen C, Tan W, Chen Y, Dong Y
Biochemical and biophysical research communications454(1)215-20(2014 Nov)

Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease.
Roman?-Aumedes J, Canal M, Mart?n-Flores N, Sun X, P?rez-Fern?ndez V, Wewering S, Fern?ndez-Santiago R, Ezquerra M, Pont-Sunyer C, Lafuente A, Alberch J, Luebbert H, Tolosa E, Levy OA, Greene LA, Malagelada C
Cell death & disease5e1364(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Redd1 expressions is markedly changed in the hippocampal CA1 region following chronic cerebral hypoperfusion.
Title: Time-Course Change of Redd1 Expressions in the Hippocampal CA1 Region Following Chronic Cerebral Hypoperfusion.

RTP801 is a novel downstream effector of mutant huntingtin -induced toxicity and that it may be relevant to the human disease.
Title: RTP801 Is Involved in Mutant Huntingtin-Induced Cell Death.

In conclusion, miRNA-221 exerts cytoprotective effects in hypoxia-reoxygenation injury in association with alterations in autophagic cell injury. Mir-221 may constitute is a novel therapeutic target in the treatment of cardiac I/R injury.
Title: Up-regulation of miRNA-221 inhibits hypoxia/reoxygenation-induced autophagy through the DDIT4/mTORC1 and Tp53inp1/p62 pathways.

REDD1 knockdown impaired autophagy in hypertrophied cardiomyocytes.
Title: REDD1 attenuates cardiac hypertrophy via enhancing autophagy.

RTP801 is a novel parkin substrate that may contribute to neurodegeneration caused by loss of parkin expression or activity.
Title: Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease.

The following Ddit4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddit4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13379	NM_080906.2 Latest version!	Rattus norvegicus DNA-damage-inducible transcript 4 (Ddit4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddit4 ( NM_080906.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_543182.1 Rattus norvegicus DNA-damage-inducible transcript 4 (Ddit4), mRNA.

Price & Availability↑

CloneID	ORa13379
Clone ID Related Accession (Same CDS sequence)	NM_080906.2
Accession Version	NM_080906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	DNA damage-inducible transcript 4 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF334162.1. On Feb 21, 2013 this sequence version replaced NM_080906.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF334162.1, BC062021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00632251, SAMN00632252 [ECO:0000350] ##Evidence-Data-END##

ATGCCTAGCC TTTGGGATCG TTTCTCGTCC TCCTCTTCCT CTTCGTCCTC GTCCCGAACT 
CCGGCCGCTG ATCGGCCGCC GCGCTCCGCC TGGGGGTCTG CGGCCAGAGA AGAGGGCCTT 
GACCGCTGCG CGAGCCTGGA GAGCTCGGAC TGCGAGTCCC TGGACAGCAG CAACAGTGGC 
TTTGGGCCGG AGGAAGACTC CTCATACCTG GATGGGGTGT CTCTGCCTGA CTTTGAGCTG 
CTCAGTGACC CCGAGGATGA GCACCTGTGT GCCAACCTGA TGCAGCTGCT GCAGGAGAGC 
CTGTCCCAGG CGCGATTGGG CTCGCGGCGC CCTGCGCGCC TGCTGATGCC GAGCCAGCTG 
TTGAGCCAGG TGGGCAAGGA ACTCCTGCGC CTGGCGTACA GCGAGCCGTG CGGCCTGCGG 
GGGGCACTGC TGGACGTCTG TGTGGAGCAA GGCAAGAGCT GCCATAGTGT GGCTCAGCTG 
GCTCTGGACC CCAGTCTAGT GCCCACCTTT CAGTTGACCC TGGTGCTGCG TCTGGACTCT 
CGCCTCTGGC CCAAGATCCA GGGCCTGTTG AGTTCTGCCA ACTCTTCCTT GGTCCCTGGT 
TACAGCCAGT CCCTGACGCT GAGCACCGGC TTCAGAGTCA TCAAAAAGAA ACTCTACAGC 
TCCGAGCAGC TGCTCATTGA AGAGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543182.1
CDS	183..872
Translation	MPSLWDRFSSSSSSSSSSRTPAADRPPRSAWGSAAREEGLDRCASLESSDCESLDSSNSGFGPEEDSSYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLSQARLGSRRPARLLMPSQLLSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVAQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLLSSANSSLVPGYSQSLTLSTGFRVIKKKLYSSEQLLIEEC

Target ORF information:

RefSeq Version	NM_080906.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA-damage-inducible transcript 4 (Ddit4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080906.2

ORF Insert Sequence:

ATGCCTAGCC TTTGGGATCG TTTCTCGTCC TCCTCTTCCT CTTCGTCCTC GTCCCGAACT 
CCGGCCGCTG ATCGGCCGCC GCGCTCCGCC TGGGGGTCTG CGGCCAGAGA AGAGGGCCTT 
GACCGCTGCG CGAGCCTGGA GAGCTCGGAC TGCGAGTCCC TGGACAGCAG CAACAGTGGC 
TTTGGGCCGG AGGAAGACTC CTCATACCTG GATGGGGTGT CTCTGCCTGA CTTTGAGCTG 
CTCAGTGACC CCGAGGATGA GCACCTGTGT GCCAACCTGA TGCAGCTGCT GCAGGAGAGC 
CTGTCCCAGG CGCGATTGGG CTCGCGGCGC CCTGCGCGCC TGCTGATGCC GAGCCAGCTG 
TTGAGCCAGG TGGGCAAGGA ACTCCTGCGC CTGGCGTACA GCGAGCCGTG CGGCCTGCGG 
GGGGCACTGC TGGACGTCTG TGTGGAGCAA GGCAAGAGCT GCCATAGTGT GGCTCAGCTG 
GCTCTGGACC CCAGTCTAGT GCCCACCTTT CAGTTGACCC TGGTGCTGCG TCTGGACTCT 
CGCCTCTGGC CCAAGATCCA GGGCCTGTTG AGTTCTGCCA ACTCTTCCTT GGTCCCTGGT 
TACAGCCAGT CCCTGACGCT GAGCACCGGC TTCAGAGTCA TCAAAAAGAA ACTCTACAGC 
TCCGAGCAGC TGCTCATTGA AGAGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Time-Course Change of Redd1 Expressions in the Hippocampal CA1 Region Following Chronic Cerebral Hypoperfusion.
Cellular and molecular neurobiology37(3)563-569(2017 Apr)
Park JA,Lee CH

RTP801 Is Involved in Mutant Huntingtin-Induced Cell Death.
Molecular neurobiology53(5)2857-2868(2016 07)
Mart?n-Flores N,Roman?-Aumedes J,Ru? L,Canal M,Sanders P,Straccia M,Allen ND,Alberch J,Canals JM,P?rez-Navarro E,Malagelada C

Up-regulation of miRNA-221 inhibits hypoxia/reoxygenation-induced autophagy through the DDIT4/mTORC1 and Tp53inp1/p62 pathways.
Biochemical and biophysical research communications474(1)168-174(2016 05)
Chen Q,Zhou Y,Richards AM,Wang P

REDD1 attenuates cardiac hypertrophy via enhancing autophagy.
Biochemical and biophysical research communications454(1)215-20(2014 Nov)
Liu C,Xue R,Wu D,Wu L,Chen C,Tan W,Chen Y,Dong Y

Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease.
Cell death & disease5e1364(2014 Aug)
Roman?-Aumedes J,Canal M,Mart?n-Flores N,Sun X,P?rez-Fern?ndez V,Wewering S,Fern?ndez-Santiago R,Ezquerra M,Pont-Sunyer C,Lafuente A,Alberch J,Luebbert H,Tolosa E,Levy OA,Greene LA,Malagelada C

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Ddit4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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