Gabra4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gabra4
Entrez Gene ID	140675
Full Name	gamma-aminobutyric acid type A receptor alpha4 subunit
General protein information	Preferred Names gamma-aminobutyric acid type A receptor alpha4 subunit Names gamma-aminobutyric acid receptor subunit alpha-4 GABA(A) receptor subunit alpha-4 GABA-A receptor alpha-4 subunit gamma-aminobutyric acid (GABA) A receptor, alpha 4 gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	alpha 4 subunit of the GABA-A receptor which binds gamma-aminobutyric acid; involved in mood and anxiety [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770135.2	XP_008768357.1	gamma-aminobutyric acid receptor subunit alpha-4 isoform X2
XM_017599056.1	XP_017454545.1	gamma-aminobutyric acid receptor subunit alpha-4 isoform X1
NM_080587.3	NP_542154.3	gamma-aminobutyric acid receptor subunit alpha-4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gabra4	NP_542154.3
Danio rerio (zebrafish)	zgc:110204	NP_001017822.1
Homo sapiens (human)	GABRA4	NP_000800.2
Bos taurus (cattle)	GABRA4	NP_776968.1
Pan troglodytes (chimpanzee)	GABRA4	XP_526774.2
Mus musculus (house mouse)	Gabra4	NP_034381.1
Canis lupus familiaris (dog)	GABRA4	XP_005628380.1
	GABRA4	XP_001101231.1
Gallus gallus (chicken)	GABRA4	XP_420724.2

Gene Ontology
Bibliography

Related articles in PubMed

?4??-GABA
Aoki C, Chen YW, Chowdhury TG, Piper W
Journal of neuroscience research(2017 Feb)

Toward Understanding Functional Properties and Subunit Arrangement of ?4?2? ?-Aminobutyric Acid, Type A (GABAA) Receptors.
Wongsamitkul N, Baur R, Sigel E
The Journal of biological chemistry291(35)18474-83(2016 08)

Effect of spontaneous seizures on GABAA receptor ?4 subunit expression in an animal model of temporal lobe epilepsy.
Grabenstatter HL, Cogswell M, Cruz Del Angel Y, Carlsen J, Gonzalez MI, Raol YH, Russek SJ, Brooks-Kayal AR
Epilepsia55(11)1826-33(2014 Nov)

Neonatal finasteride administration alters hippocampal ?4 and ? GABAAR subunits expression and behavioural responses to progesterone in adult rats.
Modol L, Casas C, Navarro X, Llid? A, Vall?e M, Pallar?s M, Darbra S
The international journal of neuropsychopharmacology17(2)259-73(2014 Feb)

The influence of stress at puberty on mood and learning: role of the ?4?? GABAA receptor.
Smith SS
Neuroscience249192-213(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the subunit arrangement of alpha4beta2delta GABAA receptors is not strongly predefined but is mostly satisfying the 2:2:1 subunit stoichiometry for alpha4, beta2, and delta subunits and that several subunit arrangements result in receptors with similar functional properties tuned to physiological conditions.
Title: Toward Understanding Functional Properties and Subunit Arrangement of ?4?2? ?-Aminobutyric Acid, Type A (GABAA) Receptors.

spontaneous, recurrent seizures that define chronic epilepsy may influence changes in GABAA receptor alpha4 expression
Title: Effect of spontaneous seizures on GABAA receptor ?4 subunit expression in an animal model of temporal lobe epilepsy.

The expression of Gabra4 in neonatal hippocampus was chaged in response to alterations in neurosteroid levels.
Title: Neonatal finasteride administration alters hippocampal ?4 and ? GABAAR subunits expression and behavioural responses to progesterone in adult rats.

alpha4betadelta GABA receptors play a role in mood and cognition at puberty in female mice.
Title: The influence of stress at puberty on mood and learning: role of the ?4?? GABAA receptor.

Adolescent female rats exhibiting activity-based anorexia express elevated levels of GABAA receptor alpha4 subunit at the plasma membrane of hippocampal CA1 spines
Title: Adolescent female rats exhibiting activity-based anorexia express elevated levels of GABA(A) receptor ?4 and ? subunits at the plasma membrane of hippocampal CA1 spines.

The following Gabra4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gabra4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12784	NM_080587.3 Latest version!	Rattus norvegicus gamma-aminobutyric acid type A receptor subunit alpha 4 (Gabra4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
ORa44177	XM_008770135.2 Latest version!	Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa58209	XM_017599056.1 Latest version!	Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gabra4 ( NM_080587.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_542154.3 Rattus norvegicus gamma-aminobutyric acid type A receptor subunit alpha 4 (Gabra4), mRNA.

Price & Availability↑

CloneID	ORa12784
Clone ID Related Accession (Same CDS sequence)	NM_080587.3
Accession Version	NM_080587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	gamma-aminobutyric acid receptor subunit alpha-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S55933.1. On Apr 28, 2006 this sequence version replaced NM_080587.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S55933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTTTCTG TCCAGAAGGT ACCTGCGATC GTGCTGTGCT CCGGGGTCAG CCTCGCCCTC 
CTGCACGTCC TGTGCCTGGC GACTTGTTTA AACGAATCCC CAGGACAGAA TTCAAAGGAC 
GAGAAATTGT GCCCGGAAAA TTTTACCCGT ATCCTGGACA GTTTGCTGGA TGGTTATGAC 
AACAGACTGC GTCCTGGATT TGGGGGTCCT GTTACAGAGG TGAAAACTGA TATATATGTC 
ACCAGCTTTG GACCCGTTTC TGATGTTGAA ATGGAATACA CAATGGATGT GTTCTTCAGA 
CAGACATGGA TTGACAAAAG ACTGAAATAT GATGGCCCCA TTGAAATCCT GAGGTTGAAC 
AATATGATGG TCACCAAAGT TTGGACCCCT GATACTTTCT TCAGGAATGG AAAGAAATCT 
GTCTCCCATA ACATGACAGC TCCAAATAAA CTTTTTAGAA TTATGAGAAA TGGCACTATT 
TTATACACAA TGAGACTCAC CATAAGTGCG GAGTGTCCCA TGAGACTGGT GGATTTTCCT 
ATGGACGGTC ATGCCTGCCC TTTGAAATTT GGGAGTTATG CATATCCCAA GAGTGAGATG 
ATCTACACCT GGACCAAAGG CCCTGAGAAG TCAGTGGAGG TACCAAAGGA GTCCTCGAGC 
TTAGTTCAGT ATGATCTAAT TGGCCAGACT GTATCCAGTG AGACTATCAA ATCTATTACA 
GGTGAATACA TTGTTATGAC CGTGTACTTT CACCTCAGAC GGAAGATGGG CTATTTTATG 
ATTCAGACAT ATATCCCGTG CATCATGACA GTGATCCTTT CTCAAGTTTC CTTCTGGATC 
AATAAGGAGT CTGTTCCAGC CAGAACTGTA TTTGGAATAA CCACAGTCCT CACGATGACC 
ACCCTAAGCA TCAGTGCTCG GCATTCTTTG CCCAAAGTGT CCTATGCGAC TGCCATGGAT 
TGGTTCATAG CTGTCTGTTT TGCTTTTGTA TTTTCGGCTC TTATTGAGTT TGCTGCTGTC 
AACTATTTCA CCAACATTCA AATGCAAAAA GCCAAAAAGA AGATATCAAA ACCTCCTCCA 
GAAGTTCCAG CTGCCCCAGT ACTGAAGGAA AAACATACAG AAACATCTCT TCAGAATACA 
CATGCTAATT TGAACATGAG GAAAAGAACA AATGCATTAG TCCACTCAGA ATCAGATGTC 
AACAGCAGAA CTGAGGTGGG GAACCATTCC AGCAAGACCA CCGCTGCCCA GGAGTCTTCT 
GAAACCACTC CTAAGGCCCA CTTGGCTTCC AGTCCAAATC CATTCAGCAG GGCAAATGCA 
GCTGAGACTA TCTCTGCAGC AGCAAGAGGT CTTTCGTCTG CCGCATCGCC CTCTCCTCAC 
GGGACACTGC AGCCAGCTCC TTTGCGGTCG GCGTCTGCTC GCCCGGCATT TGGAGCTAGA 
CTTGGGCGCA TTAAGACAAC AGTTAATACG ACAGGGGTGC CTGGGAATGT GTCAGCCACA 
CCTCCTCCCT CTGCTCCACC ACCTTCTGGA TCTGGCACAA GTAAAATAGA CAAATATGCT 
CGTATTCTCT TTCCAGTCAC ATTTGGGGCA TTTAACATGG TCTACTGGGT GGTTTATTTA 
TCTAAGGACA CCATGGAGAA ATCAGAAAGT CTAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542154.3
CDS	92..1750
Translation	MVSVQKVPAIVLCSGVSLALLHVLCLATCLNESPGQNSKDEKLCPENFTRILDSLLDGYDNRLRPGFGGPVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWIDKRLKYDGPIEILRLNNMMVTKVWTPDTFFRNGKKSVSHNMTAPNKLFRIMRNGTILYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPEKSVEVPKESSSLVQYDLIGQTVSSETIKSITGEYIVMTVYFHLRRKMGYFMIQTYIPCIMTVILSQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNIQMQKAKKKISKPPPEVPAAPVLKEKHTETSLQNTHANLNMRKRTNALVHSESDVNSRTEVGNHSSKTTAAQESSETTPKAHLASSPNPFSRANAAETISAAARGLSSAASPSPHGTLQPAPLRSASARPAFGARLGRIKTTVNTTGVPGNVSATPPPSAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM

Target ORF information:

RefSeq Version	NM_080587.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-aminobutyric acid type A receptor subunit alpha 4 (Gabra4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080587.3

ORF Insert Sequence:

ATGGTTTCTG TCCAGAAGGT ACCTGCGATC GTGCTGTGCT CCGGGGTCAG CCTCGCCCTC 
CTGCACGTCC TGTGCCTGGC GACTTGTTTA AACGAATCCC CAGGACAGAA TTCAAAGGAC 
GAGAAATTGT GCCCGGAAAA TTTTACCCGT ATCCTGGACA GTTTGCTGGA TGGTTATGAC 
AACAGACTGC GTCCTGGATT TGGGGGTCCT GTTACAGAGG TGAAAACTGA TATATATGTC 
ACCAGCTTTG GACCCGTTTC TGATGTTGAA ATGGAATACA CAATGGATGT GTTCTTCAGA 
CAGACATGGA TTGACAAAAG ACTGAAATAT GATGGCCCCA TTGAAATCCT GAGGTTGAAC 
AATATGATGG TCACCAAAGT TTGGACCCCT GATACTTTCT TCAGGAATGG AAAGAAATCT 
GTCTCCCATA ACATGACAGC TCCAAATAAA CTTTTTAGAA TTATGAGAAA TGGCACTATT 
TTATACACAA TGAGACTCAC CATAAGTGCG GAGTGTCCCA TGAGACTGGT GGATTTTCCT 
ATGGACGGTC ATGCCTGCCC TTTGAAATTT GGGAGTTATG CATATCCCAA GAGTGAGATG 
ATCTACACCT GGACCAAAGG CCCTGAGAAG TCAGTGGAGG TACCAAAGGA GTCCTCGAGC 
TTAGTTCAGT ATGATCTAAT TGGCCAGACT GTATCCAGTG AGACTATCAA ATCTATTACA 
GGTGAATACA TTGTTATGAC CGTGTACTTT CACCTCAGAC GGAAGATGGG CTATTTTATG 
ATTCAGACAT ATATCCCGTG CATCATGACA GTGATCCTTT CTCAAGTTTC CTTCTGGATC 
AATAAGGAGT CTGTTCCAGC CAGAACTGTA TTTGGAATAA CCACAGTCCT CACGATGACC 
ACCCTAAGCA TCAGTGCTCG GCATTCTTTG CCCAAAGTGT CCTATGCGAC TGCCATGGAT 
TGGTTCATAG CTGTCTGTTT TGCTTTTGTA TTTTCGGCTC TTATTGAGTT TGCTGCTGTC 
AACTATTTCA CCAACATTCA AATGCAAAAA GCCAAAAAGA AGATATCAAA ACCTCCTCCA 
GAAGTTCCAG CTGCCCCAGT ACTGAAGGAA AAACATACAG AAACATCTCT TCAGAATACA 
CATGCTAATT TGAACATGAG GAAAAGAACA AATGCATTAG TCCACTCAGA ATCAGATGTC 
AACAGCAGAA CTGAGGTGGG GAACCATTCC AGCAAGACCA CCGCTGCCCA GGAGTCTTCT 
GAAACCACTC CTAAGGCCCA CTTGGCTTCC AGTCCAAATC CATTCAGCAG GGCAAATGCA 
GCTGAGACTA TCTCTGCAGC AGCAAGAGGT CTTTCGTCTG CCGCATCGCC CTCTCCTCAC 
GGGACACTGC AGCCAGCTCC TTTGCGGTCG GCGTCTGCTC GCCCGGCATT TGGAGCTAGA 
CTTGGGCGCA TTAAGACAAC AGTTAATACG ACAGGGGTGC CTGGGAATGT GTCAGCCACA 
CCTCCTCCCT CTGCTCCACC ACCTTCTGGA TCTGGCACAA GTAAAATAGA CAAATATGCT 
CGTATTCTCT TTCCAGTCAC ATTTGGGGCA TTTAACATGG TCTACTGGGT GGTTTATTTA 
TCTAAGGACA CCATGGAGAA ATCAGAAAGT CTAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gabra4 ( XM_008770135.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768357.1 Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44177
Clone ID Related Accession (Same CDS sequence)	XM_008770135.2
Accession Version	XM_008770135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gamma-aminobutyric acid receptor subunit alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATACA CAATGGATGT GTTCTTCAGA CAGACATGGA TTGACAAAAG ACTGAAATAT 
GATGGCCCCA TTGAAATCCT GAGGTTGAAC AATATGATGG TCACCAAAGT TTGGACCCCT 
GATACTTTCT TCAGGAATGG AAAGAAATCT GTCTCCCATA ACATGACAGC TCCAAATAAA 
CTTTTTAGAA TTATGAGAAA TGGCACTATT TTATACACAA TGAGACTCAC CATAAGTGCG 
GAGTGTCCCA TGAGACTGGT GGATTTTCCT ATGGACGGTC ATGCCTGCCC TTTGAAATTT 
GGGAGTTATG CATATCCCAA GAGTGAGATG ATCTACACCT GGACCAAAGG CCCTGAGAAG 
TCAGTGGAGG TACCAAAGGA GTCCTCGAGC TTAGTTCAGT ATGATCTAAT TGGCCAGACT 
GTATCCAGTG AGACTATCAA ATCTATTACA GGTGAATACA TTGTTATGAC CGTGTACTTT 
CACCTCAGAC GGAAGATGGG CTATTTTATG ATTCAGACAT ATATCCCGTG CATCATGACA 
GTGATCCTTT CTCAAGTTTC CTTCTGGATC AATAAGGAGT CTGTTCCAGC CAGAACTGTA 
TTTGGAATAA CCACAGTCCT CACGATGACC ACCCTAAGCA TCAGTGCTCG GCATTCTTTG 
CCCAAAGTGT CCTATGCGAC TGCCATGGAT TGGTTCATAG CTGTCTGTTT TGCTTTTGTA 
TTTTCGGCTC TTATTGAGTT TGCTGCTGTC AACTATTTCA CCAACATTCA AATGCAAAAA 
GCCAAAAAGA AGATATCAAA ACCTCCTCCA GAAGTTCCAG CTGCCCCAGT ACTGAAGGAA 
AAACATACAG AAACATCTCT TCAGAATACA CATGCTAATT TGAACATGAG GAAAAGAACA 
AATGCATTAG TCCACTCAGA ATCAGATGTC AACAGCAGAA CTGAGGTGGG GAACCATTCC 
AGCAAGACCA CCGCTGCCCA GGAGTCTTCT GAAACCACTC CTAAGGCCCA CTTGGCTTCC 
AGTCCAAATC CATTCAGCAG GGCAAATGCA GCTGAGACTA TCTCTGCAGC AGCAAGAGGT 
CTTTCGTCTG CCGCATCGCC CTCTCCTCAC GGGACACTGC AGCCAGCTCC TTTGCGGTCG 
GCGTCTGCTC GCCCGGCATT TGGAGCTAGA CTTGGGCGCA TTAAGACAAC AGTTAATACG 
ACAGGGGTGC CTGGGAATGT GTCAGCCACA CCTCCTCCCT CTGCTCCACC ACCTTCTGGA 
TCTGGCACAA GTAAAATAGA CAAATATGCT CGTATTCTCT TTCCAGTCAC ATTTGGGGCA 
TTTAACATGG TCTACTGGGT GGTTTATTTA TCTAAGGACA CCATGGAGAA ATCAGAAAGT 
CTAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768357.1
CDS	2676..4064
Translation	MEYTMDVFFRQTWIDKRLKYDGPIEILRLNNMMVTKVWTPDTFFRNGKKSVSHNMTAPNKLFRIMRNGTILYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPEKSVEVPKESSSLVQYDLIGQTVSSETIKSITGEYIVMTVYFHLRRKMGYFMIQTYIPCIMTVILSQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNIQMQKAKKKISKPPPEVPAAPVLKEKHTETSLQNTHANLNMRKRTNALVHSESDVNSRTEVGNHSSKTTAAQESSETTPKAHLASSPNPFSRANAAETISAAARGLSSAASPSPHGTLQPAPLRSASARPAFGARLGRIKTTVNTTGVPGNVSATPPPSAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM

Target ORF information:

RefSeq Version	XM_008770135.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770135.2

ORF Insert Sequence:

ATGGAATACA CAATGGATGT GTTCTTCAGA CAGACATGGA TTGACAAAAG ACTGAAATAT 
GATGGCCCCA TTGAAATCCT GAGGTTGAAC AATATGATGG TCACCAAAGT TTGGACCCCT 
GATACTTTCT TCAGGAATGG AAAGAAATCT GTCTCCCATA ACATGACAGC TCCAAATAAA 
CTTTTTAGAA TTATGAGAAA TGGCACTATT TTATACACAA TGAGACTCAC CATAAGTGCG 
GAGTGTCCCA TGAGACTGGT GGATTTTCCT ATGGACGGTC ATGCCTGCCC TTTGAAATTT 
GGGAGTTATG CATATCCCAA GAGTGAGATG ATCTACACCT GGACCAAAGG CCCTGAGAAG 
TCAGTGGAGG TACCAAAGGA GTCCTCGAGC TTAGTTCAGT ATGATCTAAT TGGCCAGACT 
GTATCCAGTG AGACTATCAA ATCTATTACA GGTGAATACA TTGTTATGAC CGTGTACTTT 
CACCTCAGAC GGAAGATGGG CTATTTTATG ATTCAGACAT ATATCCCGTG CATCATGACA 
GTGATCCTTT CTCAAGTTTC CTTCTGGATC AATAAGGAGT CTGTTCCAGC CAGAACTGTA 
TTTGGAATAA CCACAGTCCT CACGATGACC ACCCTAAGCA TCAGTGCTCG GCATTCTTTG 
CCCAAAGTGT CCTATGCGAC TGCCATGGAT TGGTTCATAG CTGTCTGTTT TGCTTTTGTA 
TTTTCGGCTC TTATTGAGTT TGCTGCTGTC AACTATTTCA CCAACATTCA AATGCAAAAA 
GCCAAAAAGA AGATATCAAA ACCTCCTCCA GAAGTTCCAG CTGCCCCAGT ACTGAAGGAA 
AAACATACAG AAACATCTCT TCAGAATACA CATGCTAATT TGAACATGAG GAAAAGAACA 
AATGCATTAG TCCACTCAGA ATCAGATGTC AACAGCAGAA CTGAGGTGGG GAACCATTCC 
AGCAAGACCA CCGCTGCCCA GGAGTCTTCT GAAACCACTC CTAAGGCCCA CTTGGCTTCC 
AGTCCAAATC CATTCAGCAG GGCAAATGCA GCTGAGACTA TCTCTGCAGC AGCAAGAGGT 
CTTTCGTCTG CCGCATCGCC CTCTCCTCAC GGGACACTGC AGCCAGCTCC TTTGCGGTCG 
GCGTCTGCTC GCCCGGCATT TGGAGCTAGA CTTGGGCGCA TTAAGACAAC AGTTAATACG 
ACAGGGGTGC CTGGGAATGT GTCAGCCACA CCTCCTCCCT CTGCTCCACC ACCTTCTGGA 
TCTGGCACAA GTAAAATAGA CAAATATGCT CGTATTCTCT TTCCAGTCAC ATTTGGGGCA 
TTTAACATGG TCTACTGGGT GGTTTATTTA TCTAAGGACA CCATGGAGAA ATCAGAAAGT 
CTAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gabra4 ( XM_017599056.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454545.1 Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58209
Clone ID Related Accession (Same CDS sequence)	XM_017599056.1
Accession Version	XM_017599056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gamma-aminobutyric acid receptor subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG TCCAGAAGGT ACCTGCGATC GTGCTGTGCT CCGGGGTCAG CCTCGCCCTC 
CTGCACGTCC TGTGCCTGGC GACTTGTTTA AACGAATCCC CAGGACAGAA TTCAAAGGAC 
GAGAAATTGT GCCCGGAAAA TTTTACCCGT ATCCTGGACA GTTTGCTGGA TGGTTATGAC 
AACAGACTGC GTCCTGGATT TGGGGGTACG GATTGTTTCA AAACATGCGA GTGTGCTCTG 
TCTGTCCGTC TCTGGTCTGC CTGTCTATTT GTGGAAATAT CATTAGGTCC TGTTACAGAG 
GTGAAAACTG ATATATATGT CACCAGCTTT GGACCCGTTT CTGATGTTGA AATGGAATAC 
ACAATGGATG TGTTCTTCAG ACAGACATGG ATTGACAAAA GACTGAAATA TGATGGCCCC 
ATTGAAATCC TGAGGTTGAA CAATATGATG GTCACCAAAG TTTGGACCCC TGATACTTTC 
TTCAGGAATG GAAAGAAATC TGTCTCCCAT AACATGACAG CTCCAAATAA ACTTTTTAGA 
ATTATGAGAA ATGGCACTAT TTTATACACA ATGAGACTCA CCATAAGTGC GGAGTGTCCC 
ATGAGACTGG TGGATTTTCC TATGGACGGT CATGCCTGCC CTTTGAAATT TGGGAGTTAT 
GCATATCCCA AGAGTGAGAT GATCTACACC TGGACCAAAG GCCCTGAGAA GTCAGTGGAG 
GTACCAAAGG AGTCCTCGAG CTTAGTTCAG TATGATCTAA TTGGCCAGAC TGTATCCAGT 
GAGACTATCA AATCTATTAC AGGTGAATAC ATTGTTATGA CCGTGTACTT TCACCTCAGA 
CGGAAGATGG GCTATTTTAT GATTCAGACA TATATCCCGT GCATCATGAC AGTGATCCTT 
TCTCAAGTTT CCTTCTGGAT CAATAAGGAG TCTGTTCCAG CCAGAACTGT ATTTGGAATA 
ACCACAGTCC TCACGATGAC CACCCTAAGC ATCAGTGCTC GGCATTCTTT GCCCAAAGTG 
TCCTATGCGA CTGCCATGGA TTGGTTCATA GCTGTCTGTT TTGCTTTTGT ATTTTCGGCT 
CTTATTGAGT TTGCTGCTGT CAACTATTTC ACCAACATTC AAATGCAAAA AGCCAAAAAG 
AAGATATCAA AACCTCCTCC AGAAGTTCCA GCTGCCCCAG TACTGAAGGA AAAACATACA 
GAAACATCTC TTCAGAATAC ACATGCTAAT TTGAACATGA GGAAAAGAAC AAATGCATTA 
GTCCACTCAG AATCAGATGT CAACAGCAGA ACTGAGGTGG GGAACCATTC CAGCAAGACC 
ACCGCTGCCC AGGAGTCTTC TGAAACCACT CCTAAGGCCC ACTTGGCTTC CAGTCCAAAT 
CCATTCAGCA GGGCAAATGC AGCTGAGACT ATCTCTGCAG CAGCAAGAGG TCTTTCGTCT 
GCCGCATCGC CCTCTCCTCA CGGGACACTG CAGCCAGCTC CTTTGCGGTC GGCGTCTGCT 
CGCCCGGCAT TTGGAGCTAG ACTTGGGCGC ATTAAGACAA CAGTTAATAC GACAGGGGTG 
CCTGGGAATG TGTCAGCCAC ACCTCCTCCC TCTGCTCCAC CACCTTCTGG ATCTGGCACA 
AGTAAAATAG ACAAATATGC TCGTATTCTC TTTCCAGTCA CATTTGGGGC ATTTAACATG 
GTCTACTGGG TGGTTTATTT ATCTAAGGAC ACCATGGAGA AATCAGAAAG TCTAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454545.1
CDS	347..2086
Translation	MVSVQKVPAIVLCSGVSLALLHVLCLATCLNESPGQNSKDEKLCPENFTRILDSLLDGYDNRLRPGFGGTDCFKTCECALSVRLWSACLFVEISLGPVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWIDKRLKYDGPIEILRLNNMMVTKVWTPDTFFRNGKKSVSHNMTAPNKLFRIMRNGTILYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPEKSVEVPKESSSLVQYDLIGQTVSSETIKSITGEYIVMTVYFHLRRKMGYFMIQTYIPCIMTVILSQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNIQMQKAKKKISKPPPEVPAAPVLKEKHTETSLQNTHANLNMRKRTNALVHSESDVNSRTEVGNHSSKTTAAQESSETTPKAHLASSPNPFSRANAAETISAAARGLSSAASPSPHGTLQPAPLRSASARPAFGARLGRIKTTVNTTGVPGNVSATPPPSAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM

Target ORF information:

RefSeq Version	XM_017599056.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-aminobutyric acid type A receptor alpha4 subunit (Gabra4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599056.1

ORF Insert Sequence:

ATGGTTTCTG TCCAGAAGGT ACCTGCGATC GTGCTGTGCT CCGGGGTCAG CCTCGCCCTC 
CTGCACGTCC TGTGCCTGGC GACTTGTTTA AACGAATCCC CAGGACAGAA TTCAAAGGAC 
GAGAAATTGT GCCCGGAAAA TTTTACCCGT ATCCTGGACA GTTTGCTGGA TGGTTATGAC 
AACAGACTGC GTCCTGGATT TGGGGGTACG GATTGTTTCA AAACATGCGA GTGTGCTCTG 
TCTGTCCGTC TCTGGTCTGC CTGTCTATTT GTGGAAATAT CATTAGGTCC TGTTACAGAG 
GTGAAAACTG ATATATATGT CACCAGCTTT GGACCCGTTT CTGATGTTGA AATGGAATAC 
ACAATGGATG TGTTCTTCAG ACAGACATGG ATTGACAAAA GACTGAAATA TGATGGCCCC 
ATTGAAATCC TGAGGTTGAA CAATATGATG GTCACCAAAG TTTGGACCCC TGATACTTTC 
TTCAGGAATG GAAAGAAATC TGTCTCCCAT AACATGACAG CTCCAAATAA ACTTTTTAGA 
ATTATGAGAA ATGGCACTAT TTTATACACA ATGAGACTCA CCATAAGTGC GGAGTGTCCC 
ATGAGACTGG TGGATTTTCC TATGGACGGT CATGCCTGCC CTTTGAAATT TGGGAGTTAT 
GCATATCCCA AGAGTGAGAT GATCTACACC TGGACCAAAG GCCCTGAGAA GTCAGTGGAG 
GTACCAAAGG AGTCCTCGAG CTTAGTTCAG TATGATCTAA TTGGCCAGAC TGTATCCAGT 
GAGACTATCA AATCTATTAC AGGTGAATAC ATTGTTATGA CCGTGTACTT TCACCTCAGA 
CGGAAGATGG GCTATTTTAT GATTCAGACA TATATCCCGT GCATCATGAC AGTGATCCTT 
TCTCAAGTTT CCTTCTGGAT CAATAAGGAG TCTGTTCCAG CCAGAACTGT ATTTGGAATA 
ACCACAGTCC TCACGATGAC CACCCTAAGC ATCAGTGCTC GGCATTCTTT GCCCAAAGTG 
TCCTATGCGA CTGCCATGGA TTGGTTCATA GCTGTCTGTT TTGCTTTTGT ATTTTCGGCT 
CTTATTGAGT TTGCTGCTGT CAACTATTTC ACCAACATTC AAATGCAAAA AGCCAAAAAG 
AAGATATCAA AACCTCCTCC AGAAGTTCCA GCTGCCCCAG TACTGAAGGA AAAACATACA 
GAAACATCTC TTCAGAATAC ACATGCTAAT TTGAACATGA GGAAAAGAAC AAATGCATTA 
GTCCACTCAG AATCAGATGT CAACAGCAGA ACTGAGGTGG GGAACCATTC CAGCAAGACC 
ACCGCTGCCC AGGAGTCTTC TGAAACCACT CCTAAGGCCC ACTTGGCTTC CAGTCCAAAT 
CCATTCAGCA GGGCAAATGC AGCTGAGACT ATCTCTGCAG CAGCAAGAGG TCTTTCGTCT 
GCCGCATCGC CCTCTCCTCA CGGGACACTG CAGCCAGCTC CTTTGCGGTC GGCGTCTGCT 
CGCCCGGCAT TTGGAGCTAG ACTTGGGCGC ATTAAGACAA CAGTTAATAC GACAGGGGTG 
CCTGGGAATG TGTCAGCCAC ACCTCCTCCC TCTGCTCCAC CACCTTCTGG ATCTGGCACA 
AGTAAAATAG ACAAATATGC TCGTATTCTC TTTCCAGTCA CATTTGGGGC ATTTAACATG 
GTCTACTGGG TGGTTTATTT ATCTAAGGAC ACCATGGAGA AATCAGAAAG TCTAATGTAA