B3gat1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3gat1
Entrez Gene ID	117108
Full Name	beta-1,3-glucuronyltransferase 1
Synonyms	Hnk-1
General protein information	Preferred Names beta-1,3-glucuronyltransferase 1 Names galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase glcAT-P glcUAT-P glucuronosyltransferase P
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	mediates cell-cell and cell-substrate interaction and recognition during the development of the nervous system [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006242734.3	XP_006242796.1	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform X2
XM_006242733.3	XP_006242795.1	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform X1
NM_054003.1	NP_446455.1	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	B3GAT1	NP_001026804.1
Gallus gallus (chicken)	B3GAT1	NP_001264768.1
	B3GAT1	XP_001083698.2
Bos taurus (cattle)	B3GAT1	NP_001014415.1
Mus musculus (house mouse)	B3gat1	NP_084068.1
Rattus norvegicus (Norway rat)	B3gat1	NP_446455.1
Danio rerio (zebrafish)	b3gat1a	NP_001015066.1
Pan troglodytes (chimpanzee)	B3GAT1	NP_001026790.1
Xenopus tropicalis (tropical clawed frog)	LOC100037907	NP_001090809.1
Homo sapiens (human)	B3GAT1	NP_061114.2

Gene Ontology
Bibliography

Related articles in PubMed

Temporo-spatial analysis of Osterix, HNK1 and Sox10 during odontogenesis and maxillaries osteogenesis.
Tomazelli KB, Modolo F, Trentin AG, Garcez RC, Biz MT
Tissue & cell47(5)465-70(2015 Oct)

Glycosyltransferases and glycosaminoglycans in bleomycin and transforming growth factor-?1-induced pulmonary fibrosis.
Venkatesan N, Tsuchiya K, Kolb M, Farkas L, Bourhim M, Ouzzine M, Ludwig MS
American journal of respiratory cell and molecular biology50(3)583-94(2014 Mar)

Hypoxic regulation of ?-1,3-glucuronyltransferase 1 expression in nucleus pulposus cells of the rat intervertebral disc: role of hypoxia-inducible factor proteins.
Gogate SS, Nasser R, Shapiro IM, Risbud MV
Arthritis and rheumatism63(7)1950-60(2011 Jul)

Increased deposition of chondroitin/dermatan sulfate glycosaminoglycan and upregulation of ?1,3-glucuronosyltransferase I in pulmonary fibrosis.
Venkatesan N, Ouzzine M, Kolb M, Netter P, Ludwig MS
American journal of physiology. Lung cellular and molecular physiology300(2)L191-203(2011 Feb)

BMP-2 and TGF-beta stimulate expression of beta1,3-glucuronosyl transferase 1 (GlcAT-1) in nucleus pulposus cells through AP1, TonEBP, and Sp1: role of MAPKs.
Hiyama A, Gogate SS, Gajghate S, Mochida J, Shapiro IM, Risbud MV
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research25(5)1179-90(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

positive expression for Osterix and HNK1 in undifferentiated ectomesenchymal cells in early and late stages
Title: Temporo-spatial analysis of Osterix, HNK1 and Sox10 during odontogenesis and maxillaries osteogenesis.

increased expression of CS-GAGs and GlcAT-I in fibrotic lungs and fibrotic lung fibroblasts
Title: Increased deposition of chondroitin/dermatan sulfate glycosaminoglycan and upregulation of ?1,3-glucuronosyltransferase I in pulmonary fibrosis.

Results of these studies demonstrate that BMP-2 and TGF-beta regulate GlcAT-1 expression in nucleus pulposus cells through a signaling network comprising MAPK, AP1, Sp1, and TonEBP
Title: BMP-2 and TGF-beta stimulate expression of beta1,3-glucuronosyl transferase 1 (GlcAT-1) in nucleus pulposus cells through AP1, TonEBP, and Sp1: role of MAPKs.

TonEBP activation by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral disc
Title: Activation of TonEBP by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral disc.

Trifluralin-induced total hepatic UGT enzymes (2.4-fold) and mRNA expression of selected hepatic UGT isozymes (UGT1A1, 1.4-fold; UGT1A6, 6.4-fold; UGT2B1, 3.7-fold).
Title: Mechanism of trifluralin-induced thyroid tumors in rats.

The following B3gat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3gat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11301	NM_054003.1 Latest version!	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa11301	XM_006242733.3 Latest version!	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa30950	XM_006242734.3 Latest version!	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3gat1 ( NM_054003.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446455.1 Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), mRNA.

Price & Availability↑

CloneID	ORa11301
Clone ID Related Accession (Same CDS sequence)	XM_006242733.3 , NM_054003.1
Accession Version	NM_054003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D88035.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D88035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAATG AGGAGCTGTG GGCGCAGCCA GCCTTGGAGA TGCCGAAGAG AAGGGACATC 
CTCGCGATTG TCCTCATTGT GCTTCCCTGG ACACTGCTCA TCACCGTCTG GCACCAGAGC 
AGCCTCGCAC CTCTGCTTGC TGTGCACAAG GATGAGGGAA GTGACCCCCG CCATGAGGCA 
CCACCCGGTG CGGACCCTAG GGAGTACTGC ATGTCCGACC GTGACATCGT GGAGGTGGTG 
CGCACAGAGT ACGTGTACAC GAGGCCGCCA CCGTGGTCCG ACACGCTGCC CACCATCCAT 
GTGGTGACGC CCACCTACAG TAGACCGGTG CAGAAGGCAG AGCTGACGCG AATGGCCAAC 
ACGCTATTGC ATGTGCCCAA CCTTCACTGG CTGGTGGTGG AGGATGCTCC ACGTAGGACG 
CCCCTCACAG CGCGCCTGCT GCGCGACACT GGCCTCAACT ATACACACCT GCACGTAGAG 
ACACCACGCA ACTACAAGCT GCGAGGTGAC GCCCGAGACC CTCGCATCCC ACGTGGCACC 
ATGCAGCGCA ATCTGGCCCT GCGCTGGTTG CGGGAGACCT TCCCACGGAA CTCCACTCAG 
CCGGGTGTAG TGTACTTCGC AGATGACGAC AACACGTACA GTCTGGAGCT CTTTGAAGAG 
ATGCGCAGCA CAAGAAGGGT GTCCGTGTGG CCTGTGGCCT TTGTTGGCGG CCTTCGGTAT 
GAGGCCCCCC GGGTGAATGG GGCAGGGAAA GTGGTTGGCT GGAAGACAGT CTTCGACCCC 
CACCGGCCAT TTGCAATAGA CATGGCTGGA TTTGCTGTCA ACCTCCGGCT CATCTTGCAA 
CGAAGCCAGG CCTACTTTAA GCTACGTGGG GTAAAAGGAG GCTACCAGGA AAGCAGTCTC 
CTTCGAGAAC TTGTCACCCT CAATGATCTA GAGCCCAAGG CAGCAAACTG TACCAAGATC 
CTGGTCTGGC ATACGCGAAC AGAGAAGCCA GTGCTGGTGA ATGAGGGGAA GAAGGGCTTC 
ACTGACCCCT CGGTGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446455.1
CDS	195..1238
Translation	MGNEELWAQPALEMPKRRDILAIVLIVLPWTLLITVWHQSSLAPLLAVHKDEGSDPRHEAPPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSTQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

Target ORF information:

RefSeq Version	NM_054003.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054003.1

ORF Insert Sequence:

ATGGGTAATG AGGAGCTGTG GGCGCAGCCA GCCTTGGAGA TGCCGAAGAG AAGGGACATC 
CTCGCGATTG TCCTCATTGT GCTTCCCTGG ACACTGCTCA TCACCGTCTG GCACCAGAGC 
AGCCTCGCAC CTCTGCTTGC TGTGCACAAG GATGAGGGAA GTGACCCCCG CCATGAGGCA 
CCACCCGGTG CGGACCCTAG GGAGTACTGC ATGTCCGACC GTGACATCGT GGAGGTGGTG 
CGCACAGAGT ACGTGTACAC GAGGCCGCCA CCGTGGTCCG ACACGCTGCC CACCATCCAT 
GTGGTGACGC CCACCTACAG TAGACCGGTG CAGAAGGCAG AGCTGACGCG AATGGCCAAC 
ACGCTATTGC ATGTGCCCAA CCTTCACTGG CTGGTGGTGG AGGATGCTCC ACGTAGGACG 
CCCCTCACAG CGCGCCTGCT GCGCGACACT GGCCTCAACT ATACACACCT GCACGTAGAG 
ACACCACGCA ACTACAAGCT GCGAGGTGAC GCCCGAGACC CTCGCATCCC ACGTGGCACC 
ATGCAGCGCA ATCTGGCCCT GCGCTGGTTG CGGGAGACCT TCCCACGGAA CTCCACTCAG 
CCGGGTGTAG TGTACTTCGC AGATGACGAC AACACGTACA GTCTGGAGCT CTTTGAAGAG 
ATGCGCAGCA CAAGAAGGGT GTCCGTGTGG CCTGTGGCCT TTGTTGGCGG CCTTCGGTAT 
GAGGCCCCCC GGGTGAATGG GGCAGGGAAA GTGGTTGGCT GGAAGACAGT CTTCGACCCC 
CACCGGCCAT TTGCAATAGA CATGGCTGGA TTTGCTGTCA ACCTCCGGCT CATCTTGCAA 
CGAAGCCAGG CCTACTTTAA GCTACGTGGG GTAAAAGGAG GCTACCAGGA AAGCAGTCTC 
CTTCGAGAAC TTGTCACCCT CAATGATCTA GAGCCCAAGG CAGCAAACTG TACCAAGATC 
CTGGTCTGGC ATACGCGAAC AGAGAAGCCA GTGCTGGTGA ATGAGGGGAA GAAGGGCTTC 
ACTGACCCCT CGGTGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gat1 ( XM_006242733.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242795.1 Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11301
Clone ID Related Accession (Same CDS sequence)	XM_006242733.3 , NM_054003.1
Accession Version	XM_006242733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG AGGAGCTGTG GGCGCAGCCA GCCTTGGAGA TGCCGAAGAG AAGGGACATC 
CTCGCGATTG TCCTCATTGT GCTTCCCTGG ACACTGCTCA TCACCGTCTG GCACCAGAGC 
AGCCTCGCAC CTCTGCTTGC TGTGCACAAG GATGAGGGAA GTGACCCCCG CCATGAGGCA 
CCACCCGGTG CGGACCCTAG GGAGTACTGC ATGTCCGACC GTGACATCGT GGAGGTGGTG 
CGCACAGAGT ACGTGTACAC GAGGCCGCCA CCGTGGTCCG ACACGCTGCC CACCATCCAT 
GTGGTGACGC CCACCTACAG TAGACCGGTG CAGAAGGCAG AGCTGACGCG AATGGCCAAC 
ACGCTATTGC ATGTGCCCAA CCTTCACTGG CTGGTGGTGG AGGATGCTCC ACGTAGGACG 
CCCCTCACAG CGCGCCTGCT GCGCGACACT GGCCTCAACT ATACACACCT GCACGTAGAG 
ACACCACGCA ACTACAAGCT GCGAGGTGAC GCCCGAGACC CTCGCATCCC ACGTGGCACC 
ATGCAGCGCA ATCTGGCCCT GCGCTGGTTG CGGGAGACCT TCCCACGGAA CTCCACTCAG 
CCGGGTGTAG TGTACTTCGC AGATGACGAC AACACGTACA GTCTGGAGCT CTTTGAAGAG 
ATGCGCAGCA CAAGAAGGGT GTCCGTGTGG CCTGTGGCCT TTGTTGGCGG CCTTCGGTAT 
GAGGCCCCCC GGGTGAATGG GGCAGGGAAA GTGGTTGGCT GGAAGACAGT CTTCGACCCC 
CACCGGCCAT TTGCAATAGA CATGGCTGGA TTTGCTGTCA ACCTCCGGCT CATCTTGCAA 
CGAAGCCAGG CCTACTTTAA GCTACGTGGG GTAAAAGGAG GCTACCAGGA AAGCAGTCTC 
CTTCGAGAAC TTGTCACCCT CAATGATCTA GAGCCCAAGG CAGCAAACTG TACCAAGATC 
CTGGTCTGGC ATACGCGAAC AGAGAAGCCA GTGCTGGTGA ATGAGGGGAA GAAGGGCTTC 
ACTGACCCCT CGGTGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242795.1
CDS	398..1441
Translation	MGNEELWAQPALEMPKRRDILAIVLIVLPWTLLITVWHQSSLAPLLAVHKDEGSDPRHEAPPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSTQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

Target ORF information:

RefSeq Version	XM_006242733.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242733.3

ORF Insert Sequence:

ATGGGTAATG AGGAGCTGTG GGCGCAGCCA GCCTTGGAGA TGCCGAAGAG AAGGGACATC 
CTCGCGATTG TCCTCATTGT GCTTCCCTGG ACACTGCTCA TCACCGTCTG GCACCAGAGC 
AGCCTCGCAC CTCTGCTTGC TGTGCACAAG GATGAGGGAA GTGACCCCCG CCATGAGGCA 
CCACCCGGTG CGGACCCTAG GGAGTACTGC ATGTCCGACC GTGACATCGT GGAGGTGGTG 
CGCACAGAGT ACGTGTACAC GAGGCCGCCA CCGTGGTCCG ACACGCTGCC CACCATCCAT 
GTGGTGACGC CCACCTACAG TAGACCGGTG CAGAAGGCAG AGCTGACGCG AATGGCCAAC 
ACGCTATTGC ATGTGCCCAA CCTTCACTGG CTGGTGGTGG AGGATGCTCC ACGTAGGACG 
CCCCTCACAG CGCGCCTGCT GCGCGACACT GGCCTCAACT ATACACACCT GCACGTAGAG 
ACACCACGCA ACTACAAGCT GCGAGGTGAC GCCCGAGACC CTCGCATCCC ACGTGGCACC 
ATGCAGCGCA ATCTGGCCCT GCGCTGGTTG CGGGAGACCT TCCCACGGAA CTCCACTCAG 
CCGGGTGTAG TGTACTTCGC AGATGACGAC AACACGTACA GTCTGGAGCT CTTTGAAGAG 
ATGCGCAGCA CAAGAAGGGT GTCCGTGTGG CCTGTGGCCT TTGTTGGCGG CCTTCGGTAT 
GAGGCCCCCC GGGTGAATGG GGCAGGGAAA GTGGTTGGCT GGAAGACAGT CTTCGACCCC 
CACCGGCCAT TTGCAATAGA CATGGCTGGA TTTGCTGTCA ACCTCCGGCT CATCTTGCAA 
CGAAGCCAGG CCTACTTTAA GCTACGTGGG GTAAAAGGAG GCTACCAGGA AAGCAGTCTC 
CTTCGAGAAC TTGTCACCCT CAATGATCTA GAGCCCAAGG CAGCAAACTG TACCAAGATC 
CTGGTCTGGC ATACGCGAAC AGAGAAGCCA GTGCTGGTGA ATGAGGGGAA GAAGGGCTTC 
ACTGACCCCT CGGTGGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gat1 ( XM_006242734.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242796.1 Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30950
Clone ID Related Accession (Same CDS sequence)	XM_006242734.3
Accession Version	XM_006242734.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGA GAAGGGACAT CCTCGCGATT GTCCTCATTG TGCTTCCCTG GACACTGCTC 
ATCACCGTCT GGCACCAGAG CAGCCTCGCA CCTCTGCTTG CTGTGCACAA GGATGAGGGA 
AGTGACCCCC GCCATGAGGC ACCACCCGGT GCGGACCCTA GGGAGTACTG CATGTCCGAC 
CGTGACATCG TGGAGGTGGT GCGCACAGAG TACGTGTACA CGAGGCCGCC ACCGTGGTCC 
GACACGCTGC CCACCATCCA TGTGGTGACG CCCACCTACA GTAGACCGGT GCAGAAGGCA 
GAGCTGACGC GAATGGCCAA CACGCTATTG CATGTGCCCA ACCTTCACTG GCTGGTGGTG 
GAGGATGCTC CACGTAGGAC GCCCCTCACA GCGCGCCTGC TGCGCGACAC TGGCCTCAAC 
TATACACACC TGCACGTAGA GACACCACGC AACTACAAGC TGCGAGGTGA CGCCCGAGAC 
CCTCGCATCC CACGTGGCAC CATGCAGCGC AATCTGGCCC TGCGCTGGTT GCGGGAGACC 
TTCCCACGGA ACTCCACTCA GCCGGGTGTA GTGTACTTCG CAGATGACGA CAACACGTAC 
AGTCTGGAGC TCTTTGAAGA GATGCGCAGC ACAAGAAGGG TGTCCGTGTG GCCTGTGGCC 
TTTGTTGGCG GCCTTCGGTA TGAGGCCCCC CGGGTGAATG GGGCAGGGAA AGTGGTTGGC 
TGGAAGACAG TCTTCGACCC CCACCGGCCA TTTGCAATAG ACATGGCTGG ATTTGCTGTC 
AACCTCCGGC TCATCTTGCA ACGAAGCCAG GCCTACTTTA AGCTACGTGG GGTAAAAGGA 
GGCTACCAGG AAAGCAGTCT CCTTCGAGAA CTTGTCACCC TCAATGATCT AGAGCCCAAG 
GCAGCAAACT GTACCAAGAT CCTGGTCTGG CATACGCGAA CAGAGAAGCC AGTGCTGGTG 
AATGAGGGGA AGAAGGGCTT CACTGACCCC TCGGTGGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242796.1
CDS	287..1291
Translation	MPKRRDILAIVLIVLPWTLLITVWHQSSLAPLLAVHKDEGSDPRHEAPPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSTQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

Target ORF information:

RefSeq Version	XM_006242734.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,3-glucuronyltransferase 1 (B3gat1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242734.3

ORF Insert Sequence:

ATGCCGAAGA GAAGGGACAT CCTCGCGATT GTCCTCATTG TGCTTCCCTG GACACTGCTC 
ATCACCGTCT GGCACCAGAG CAGCCTCGCA CCTCTGCTTG CTGTGCACAA GGATGAGGGA 
AGTGACCCCC GCCATGAGGC ACCACCCGGT GCGGACCCTA GGGAGTACTG CATGTCCGAC 
CGTGACATCG TGGAGGTGGT GCGCACAGAG TACGTGTACA CGAGGCCGCC ACCGTGGTCC 
GACACGCTGC CCACCATCCA TGTGGTGACG CCCACCTACA GTAGACCGGT GCAGAAGGCA 
GAGCTGACGC GAATGGCCAA CACGCTATTG CATGTGCCCA ACCTTCACTG GCTGGTGGTG 
GAGGATGCTC CACGTAGGAC GCCCCTCACA GCGCGCCTGC TGCGCGACAC TGGCCTCAAC 
TATACACACC TGCACGTAGA GACACCACGC AACTACAAGC TGCGAGGTGA CGCCCGAGAC 
CCTCGCATCC CACGTGGCAC CATGCAGCGC AATCTGGCCC TGCGCTGGTT GCGGGAGACC 
TTCCCACGGA ACTCCACTCA GCCGGGTGTA GTGTACTTCG CAGATGACGA CAACACGTAC 
AGTCTGGAGC TCTTTGAAGA GATGCGCAGC ACAAGAAGGG TGTCCGTGTG GCCTGTGGCC 
TTTGTTGGCG GCCTTCGGTA TGAGGCCCCC CGGGTGAATG GGGCAGGGAA AGTGGTTGGC 
TGGAAGACAG TCTTCGACCC CCACCGGCCA TTTGCAATAG ACATGGCTGG ATTTGCTGTC 
AACCTCCGGC TCATCTTGCA ACGAAGCCAG GCCTACTTTA AGCTACGTGG GGTAAAAGGA 
GGCTACCAGG AAAGCAGTCT CCTTCGAGAA CTTGTCACCC TCAATGATCT AGAGCCCAAG 
GCAGCAAACT GTACCAAGAT CCTGGTCTGG CATACGCGAA CAGAGAAGCC AGTGCTGGTG 
AATGAGGGGA AGAAGGGCTT CACTGACCCC TCGGTGGAGA TCTGA