B3gnt5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3gnt5
Entrez Gene ID	116740
Full Name	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
General protein information	Preferred Names UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 Names lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase BGnT-5 UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5 beta-1,3-Gn-T5 beta-1,3-N-acetylglucosaminyltransferase 5 beta3Gn-T5 lactotriaosylceramide synthase lc(3)Cer synthase lc3 synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_053932.1	NP_446384.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	B3GNT5	NP_001107233.1
Danio rerio (zebrafish)	b3gnt5a	NP_942577.1
Canis lupus familiaris (dog)	B3GNT5	XP_005639817.1
Xenopus tropicalis (tropical clawed frog)	b3gnt5	NP_989240.1
	B3GNT5	XP_001106314.1
Homo sapiens (human)	B3GNT5	NP_114436.1
Pan troglodytes (chimpanzee)	B3GNT5	XP_001134746.2
Bos taurus (cattle)	B3GNT5	NP_001070447.1
Mus musculus (house mouse)	B3gnt5	NP_001152879.1
Rattus norvegicus (Norway rat)	B3gnt5	NP_446384.1

Gene Ontology
Bibliography

Related articles in PubMed

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.
Henion TR, Zhou D, Wolfer DP, Jungalwala FB, Hennet T
The Journal of biological chemistry276(32)30261-9(2001 Aug)

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.
Togayachi A, Akashima T, Ookubo R, Kudo T, Nishihara S, Iwasaki H, Natsume A, Mio H, Inokuchi J, Irimura T, Sasaki K, Narimatsu H
The Journal of biological chemistry276(25)22032-40(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following B3gnt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3gnt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11092	NM_053932.1 Latest version!	Rattus norvegicus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3gnt5 ( NM_053932.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446384.1 Rattus norvegicus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), mRNA.

Price & Availability↑

CloneID	ORa11092
Clone ID Related Accession (Same CDS sequence)	NM_053932.1
Accession Version	NM_053932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034708.1. On or before Dec 17, 2009 this sequence version replaced XM_344040.3, XM_001060426.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DN933543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGTAT TCGTCAGCAG CAGAAGAGTC AAAAGATGGC AGTTTTTTCA CTTATTTGCC 
ATTTGTTTTA TATTAAGCTT TATGGTTTTT TGGGGCCCGA TCAATAATTA CATCATGAGC 
CACATGAAGT CCTACTCCTA CAGATACCTT ATAAATAGCT ATGACTTTGT GAACGATTCC 
CTGTCTCTCA AGCACAGCTC TGTGCAGCCT CACCACCCAT ACTTGATCAA CCACAGAGAG 
AAGTGTCAGG CCCAAGATGT CCTCCTCTTA CTCTTTATAA AGACTGCCCC TGAAAACTAC 
GAGCGACGTT CTGCCATCAG AAAGACGTGG GGCAACGAGA ATTACGTCCA GTCTCAGCTC 
AACGCCAACA TCAAAATTCT GTTCGCGTTA GGAACTCCTC ATCCACTGAA GGGAAAAGAG 
CTGCAAAAAA GACTGATTTG GGAAGATCAA GTGTACCACG ACATAATTCA GCAAGATTTC 
ACTGATTCTT TCCACAATCT TACTTTTAAA TTTCTTCTTC AGTTCGGCTG GGCAAACACC 
TTTTGCCCAC ATGCCAGATT CCTGATGACT GCTGATGATG ACATATTTAT CCACATGCCA 
AATCTCATTG AATACCTTCA AGGGCTGGAG CAGGTTGGAG TTCGAGACTT TTGGATTGGT 
CACGTTCACC GAGGGGGCCC TCCTGTTAGA GACAAAAGTA GCAAGTACTA TGTTCCCTAT 
GAAATGTACA AGTGGCCAGC CTACCCTGAC TATACCGCCG GTGCTGCCTA TGTCGTCTCC 
AACGATGTAG CTGCCAAAAT CTATGAGGCA TCACAGACGC TGAATTCCAG CATGTACATA 
GACGATGTGT TCATGGGCCT CTGCGCCAAT AAAGTGGGGG TCGTGCCACA GGACCATGTA 
TTTTTCTCTG GGGAAGGGAA GATTCCTTAC CATCCCTGCA TCTATGAAAA GATGATAACG 
TCTCATGGAC ACTCACAAGA CCTACAGGAC CTCTGGGTGG AGGCCACAGA TCCTAAAGTG 
AAGGACATTT CGAAAGGGTT TTTTGGTCAG ATCTACTGCA GGTTAATTAA GATAGTTCTT 
CTCTGCAGAC TGACTTACAG GAACTCATAC CCTTGTAGGG CTGCATTTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446384.1
CDS	669..1802
Translation	MRVFVSSRRVKRWQFFHLFAICFILSFMVFWGPINNYIMSHMKSYSYRYLINSYDFVNDSLSLKHSSVQPHHPYLINHREKCQAQDVLLLLFIKTAPENYERRSAIRKTWGNENYVQSQLNANIKILFALGTPHPLKGKELQKRLIWEDQVYHDIIQQDFTDSFHNLTFKFLLQFGWANTFCPHARFLMTADDDIFIHMPNLIEYLQGLEQVGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSNDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGVVPQDHVFFSGEGKIPYHPCIYEKMITSHGHSQDLQDLWVEATDPKVKDISKGFFGQIYCRLIKIVLLCRLTYRNSYPCRAAFA

Target ORF information:

RefSeq Version	NM_053932.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053932.1

ORF Insert Sequence:

ATGAGAGTAT TCGTCAGCAG CAGAAGAGTC AAAAGATGGC AGTTTTTTCA CTTATTTGCC 
ATTTGTTTTA TATTAAGCTT TATGGTTTTT TGGGGCCCGA TCAATAATTA CATCATGAGC 
CACATGAAGT CCTACTCCTA CAGATACCTT ATAAATAGCT ATGACTTTGT GAACGATTCC 
CTGTCTCTCA AGCACAGCTC TGTGCAGCCT CACCACCCAT ACTTGATCAA CCACAGAGAG 
AAGTGTCAGG CCCAAGATGT CCTCCTCTTA CTCTTTATAA AGACTGCCCC TGAAAACTAC 
GAGCGACGTT CTGCCATCAG AAAGACGTGG GGCAACGAGA ATTACGTCCA GTCTCAGCTC 
AACGCCAACA TCAAAATTCT GTTCGCGTTA GGAACTCCTC ATCCACTGAA GGGAAAAGAG 
CTGCAAAAAA GACTGATTTG GGAAGATCAA GTGTACCACG ACATAATTCA GCAAGATTTC 
ACTGATTCTT TCCACAATCT TACTTTTAAA TTTCTTCTTC AGTTCGGCTG GGCAAACACC 
TTTTGCCCAC ATGCCAGATT CCTGATGACT GCTGATGATG ACATATTTAT CCACATGCCA 
AATCTCATTG AATACCTTCA AGGGCTGGAG CAGGTTGGAG TTCGAGACTT TTGGATTGGT 
CACGTTCACC GAGGGGGCCC TCCTGTTAGA GACAAAAGTA GCAAGTACTA TGTTCCCTAT 
GAAATGTACA AGTGGCCAGC CTACCCTGAC TATACCGCCG GTGCTGCCTA TGTCGTCTCC 
AACGATGTAG CTGCCAAAAT CTATGAGGCA TCACAGACGC TGAATTCCAG CATGTACATA 
GACGATGTGT TCATGGGCCT CTGCGCCAAT AAAGTGGGGG TCGTGCCACA GGACCATGTA 
TTTTTCTCTG GGGAAGGGAA GATTCCTTAC CATCCCTGCA TCTATGAAAA GATGATAACG 
TCTCATGGAC ACTCACAAGA CCTACAGGAC CTCTGGGTGG AGGCCACAGA TCCTAAAGTG 
AAGGACATTT CGAAAGGGTT TTTTGGTCAG ATCTACTGCA GGTTAATTAA GATAGTTCTT 
CTCTGCAGAC TGACTTACAG GAACTCATAC CCTTGTAGGG CTGCATTTGC TTAA