Efna5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Efna5
Entrez Gene ID	116683
Full Name	ephrin A5
Synonyms	Lerk7
General protein information	Preferred Names ephrin A5 Names ephrin-A5 AL-1 LERK-7 eph-related receptor tyrosine kinase ligand 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q37
Summary	Ephrin-related tryosine-kinase receptor, designated ephrin-A5 based on structure and sequence relationships; implicated in nervous system development, particularly with axon pathfinding and fasciculation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053903.1	NP_446355.1	ephrin-A5 precursor
XM_006245593.3	XP_006245655.1	ephrin-A5 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC608458	XP_850582.2
Pan troglodytes (chimpanzee)	EFNA5	XP_526972.2
Rattus norvegicus (Norway rat)	Efna5	NP_446355.1
	EFNA5	XP_001099753.1
Bos taurus (cattle)	EFNA5	NP_001069900.1
Danio rerio (zebrafish)	efna5b	NP_571101.1
Homo sapiens (human)	EFNA5	NP_001953.1
Mus musculus (house mouse)	Efna5	NP_997537.1
Gallus gallus (chicken)	EFNA5	NP_990515.1
Xenopus tropicalis (tropical clawed frog)	efna5	NP_001016422.1

Gene Ontology
Bibliography

Related articles in PubMed

[Changes in the expression of EphA5/ephrinA5 in the CA3 region of the hippocampus in rats with epilepsy and their role in the pathogenesis of temporal lobe epilepsy].
Liu TT, Xiao B, Li SY, Li GL, Lu XQ, Liu WP
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics19(12)1272-1277(2017 Dec)

Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
Szepietowska B, Horvath TL, Sherwin RS
Diabetes63(3)1140-7(2014 Mar)

EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin RS
Diabetes62(4)1282-8(2013 Apr)

The ephrin signaling pathway regulates morphology and adhesion of mouse granulosa cells in vitro.
Buensuceso AV, Deroo BJ
Biology of reproduction88(1)25(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

changes in ephrinA5/EphA5 interactions and synaptic plasticity within the ventromedial hypothalamus may contribute to the impairment in glucagon secretion and counterregulatory responses caused by recurrent hypoglycemia.
Title: Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.

Changes in ephrinA5/EphA5 interactions within the ventromedial hypothalamus act in concert with islets to restore glucose homeostasis during acute hypoglycemia, and its effect on counterregulation may be mediated by changes in glutamate/glutamine cycling.
Title: EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.

ephrin-A5 and EphA5 signals play a necessary, activity-independent role in the initiation of the early phases of synaptogenesis
Title: Ephrin-A5 and EphA5 interaction induces synaptogenesis during early hippocampal development.

preservation of a nasal(low)-temporal(high) EphA5 gradient in retinal ganglion cell in neurons in superior colliculus in nerve graft
Title: EphA5 and ephrin-A2 expression during optic nerve regeneration: a 'two-edged sword'.

Pax6-dependent boundary defines alignment of migrating olfactory cortex neurons via the repulsive activity of ephrin-A5.
Title: Pax6-dependent boundary defines alignment of migrating olfactory cortex neurons via the repulsive activity of ephrin A5.

The following Efna5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efna5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13097	NM_053903.1 Latest version!	Rattus norvegicus ephrin A5 (Efna5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa32614	XM_006245593.3 Latest version!	Rattus norvegicus ephrin A5 (Efna5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Efna5 ( NM_053903.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446355.1 Rattus norvegicus ephrin A5 (Efna5), mRNA.

Price & Availability↑

CloneID	ORa13097
Clone ID Related Accession (Same CDS sequence)	NM_053903.1
Accession Version	NM_053903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	ephrin-A5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U69279.1. On Apr 19, 2005 this sequence version replaced XM_579582.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U69279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTTGCACG TGGAGATGTT GACGCTGCTC TTTCTGGTGC TCTGGATGTG TGTGTTCAGC 
CAGGACCCGG GCTCCAAAGT CGTCGCCGAC CGCTACGCCG TCTACTGGAA CAGCAGCAAC 
CCCAGATTCC AGAGGGGTGA CTACCACATC GATGTCTGTA TCAATGACTA CCTGGATGTT 
TTCTGCCCTC ACTATGAGGA CTCTGTACCA GAGGATAAGA CTGAGCGCTA TGTCCTGTAC 
ATGGTGAATT TTGATGGCTA CAGTGCCTGC GACCACACAT CCAAAGGGTT CAAGAGATGG 
GAATGTAACC GGCCTCACTC TCCAAACGGA CCGCTGAAGT TCTCGGAGAA ATTCCAGCTC 
TTCACTCCCT TTTCTTTAGG ATTTGAATTC AGGCCAGGCC GAGAGTATTT CTACATCTCC 
TCTGCAATCC CAGACAATGG AAGAAGATCC TGCCTAAAGC TCAAAGTCTT TGTGAGGCCA 
ACAAACAGCT GTATGAAAAC TATAGGTGTT CGTGATCGTG TTTTCGATGT TAACGACAAA 
GTAGAAAATT CATTAGAACC AGCAGATGAC ACCGTACATG AGTCAGCCGA GCCATCCCGC 
GGAGAGAACG CGGCACAAAC ACCAAGGATA CCCAGCCGCC TTTTGGCAAT CCTACTGTTC 
CTCCTGGCGA TGCTTTTGAC ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446355.1
CDS	39..725
Translation	MLHVEMLTLLFLVLWMCVFSQDPGSKVVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVRDRVFDVNDKVENSLEPADDTVHESAEPSRGENAAQTPRIPSRLLAILLFLLAMLLTL

Target ORF information:

RefSeq Version	NM_053903.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ephrin A5 (Efna5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053903.1

ORF Insert Sequence:

ATGTTGCACG TGGAGATGTT GACGCTGCTC TTTCTGGTGC TCTGGATGTG TGTGTTCAGC 
CAGGACCCGG GCTCCAAAGT CGTCGCCGAC CGCTACGCCG TCTACTGGAA CAGCAGCAAC 
CCCAGATTCC AGAGGGGTGA CTACCACATC GATGTCTGTA TCAATGACTA CCTGGATGTT 
TTCTGCCCTC ACTATGAGGA CTCTGTACCA GAGGATAAGA CTGAGCGCTA TGTCCTGTAC 
ATGGTGAATT TTGATGGCTA CAGTGCCTGC GACCACACAT CCAAAGGGTT CAAGAGATGG 
GAATGTAACC GGCCTCACTC TCCAAACGGA CCGCTGAAGT TCTCGGAGAA ATTCCAGCTC 
TTCACTCCCT TTTCTTTAGG ATTTGAATTC AGGCCAGGCC GAGAGTATTT CTACATCTCC 
TCTGCAATCC CAGACAATGG AAGAAGATCC TGCCTAAAGC TCAAAGTCTT TGTGAGGCCA 
ACAAACAGCT GTATGAAAAC TATAGGTGTT CGTGATCGTG TTTTCGATGT TAACGACAAA 
GTAGAAAATT CATTAGAACC AGCAGATGAC ACCGTACATG AGTCAGCCGA GCCATCCCGC 
GGAGAGAACG CGGCACAAAC ACCAAGGATA CCCAGCCGCC TTTTGGCAAT CCTACTGTTC 
CTCCTGGCGA TGCTTTTGAC ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efna5 ( XM_006245593.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245655.1 Rattus norvegicus ephrin A5 (Efna5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32614
Clone ID Related Accession (Same CDS sequence)	XM_006245593.3
Accession Version	XM_006245593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ephrin-A5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCACG TGGAGATGTT GACGCTGCTC TTTCTGGTGC TCTGGATGTG TGTGTTCAGC 
CAGGACCCGG GCTCCAAAGT CGTCGCCGAC CGCTACGCCG TCTACTGGAA CAGCAGCAAC 
CCCAGATTCC AGAGGGGTGA CTACCACATC GATGTCTGTA TCAATGACTA CCTGGATGTT 
TTCTGCCCTC ACTATGAGGA CTCTGTACCA GAGGATAAGA CTGAGCGCTA TGTCCTGTAC 
ATGGTGAATT TTGATGGCTA CAGTGCCTGC GACCACACAT CCAAAGGGTT CAAGAGATGG 
GAATGTAACC GGCCTCACTC TCCAAACGGA CCGCTGAAGT TCTCGGAGAA ATTCCAGCTC 
TTCACTCCCT TTTCTTTAGG ATTTGAATTC AGGCCAGGCC GAGAGTATTT CTACATCTCC 
TCTGCAATCC CAGACAATGG AAGAAGATCC TGCCTAAAGC TCAAAGTCTT TGTGAGGCCA 
ACAAATGACA CCGTACATGA GTCAGCCGAG CCATCCCGCG GAGAGAACGC GGCACAAACA 
CCAAGGATAC CCAGCCGCCT TTTGGCAATC CTACTGTTCC TCCTGGCGAT GCTTTTGACA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245655.1
CDS	230..835
Translation	MLHVEMLTLLFLVLWMCVFSQDPGSKVVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNDTVHESAEPSRGENAAQTPRIPSRLLAILLFLLAMLLTL

Target ORF information:

RefSeq Version	XM_006245593.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ephrin A5 (Efna5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245593.3

ORF Insert Sequence:

ATGTTGCACG TGGAGATGTT GACGCTGCTC TTTCTGGTGC TCTGGATGTG TGTGTTCAGC 
CAGGACCCGG GCTCCAAAGT CGTCGCCGAC CGCTACGCCG TCTACTGGAA CAGCAGCAAC 
CCCAGATTCC AGAGGGGTGA CTACCACATC GATGTCTGTA TCAATGACTA CCTGGATGTT 
TTCTGCCCTC ACTATGAGGA CTCTGTACCA GAGGATAAGA CTGAGCGCTA TGTCCTGTAC 
ATGGTGAATT TTGATGGCTA CAGTGCCTGC GACCACACAT CCAAAGGGTT CAAGAGATGG 
GAATGTAACC GGCCTCACTC TCCAAACGGA CCGCTGAAGT TCTCGGAGAA ATTCCAGCTC 
TTCACTCCCT TTTCTTTAGG ATTTGAATTC AGGCCAGGCC GAGAGTATTT CTACATCTCC 
TCTGCAATCC CAGACAATGG AAGAAGATCC TGCCTAAAGC TCAAAGTCTT TGTGAGGCCA 
ACAAATGACA CCGTACATGA GTCAGCCGAG CCATCCCGCG GAGAGAACGC GGCACAAACA 
CCAAGGATAC CCAGCCGCCT TTTGGCAATC CTACTGTTCC TCCTGGCGAT GCTTTTGACA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Changes in the expression of EphA5/ephrinA5 in the CA3 region of the hippocampus in rats with epilepsy and their role in the pathogenesis of temporal lobe epilepsy].
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics19(12)1272-1277(2017 Dec)
Liu TT,Xiao B,Li SY,Li GL,Lu XQ,Liu WP

Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
Diabetes63(3)1140-7(2014 Mar)
Szepietowska B,Horvath TL,Sherwin RS

EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
Diabetes62(4)1282-8(2013 Apr)
Szepietowska B,Zhu W,Czyzyk J,Eid T,Sherwin RS

The ephrin signaling pathway regulates morphology and adhesion of mouse granulosa cells in vitro.
Biology of reproduction88(1)25(2013 Jan)
Buensuceso AV,Deroo BJ