Vcp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vcp
Entrez Gene ID	116643
Full Name	valosin-containing protein
General protein information	Preferred Names valosin-containing protein Names transitional endoplasmic reticulum ATPase 15S Mg(2+)-ATPase p97 subunit TER ATPase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	ATP binding protein involved in vesicle transport and fusion; important for proper functioning of the endoplasmic reticulum [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053864.2	NP_446316.1	transitional endoplasmic reticulum ATPase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VCP	XP_003312111.1
Gallus gallus (chicken)	VCP	NP_001038129.1
Magnaporthe oryzae (rice blast fungus)	MGG_05193	XP_003712735.1
Canis lupus familiaris (dog)	VCP	XP_852626.1
Bos taurus (cattle)	VCP	NP_001029466.1
Mus musculus (house mouse)	Vcp	NP_033529.3
Drosophila melanogaster (fruit fly)	TER94	NP_477369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005630	XP_315644.3
Saccharomyces cerevisiae (baker's yeast)	CDC48	NP_010157.1
Schizosaccharomyces pombe (fission yeast)	cdc48	NP_593287.2
Neurospora crassa	NCU00018	XP_957005.2
Arabidopsis thaliana (thale cress)	AT3G53230	NP_190891.1
Rattus norvegicus (Norway rat)	Vcp	NP_446316.1
Danio rerio (zebrafish)	vcp	NP_958889.1
Kluyveromyces lactis	KLLA0F05676g	XP_455337.1
Arabidopsis thaliana (thale cress)	AT5G03340	NP_568114.1
Oryza sativa (rice)	Os03g0151800	NP_001048987.1
Xenopus tropicalis (tropical clawed frog)	vcp	NP_001005677.1
Caenorhabditis elegans (roundworm)	cdc-48.1	NP_496273.1
Caenorhabditis elegans (roundworm)	cdc-48.2	NP_495705.1
Arabidopsis thaliana (thale cress)	CDC48	NP_187595.1
Homo sapiens (human)	VCP	NP_009057.1
Eremothecium gossypii	AGOS_AFR158W	NP_985705.2

Gene Ontology
Bibliography

Related articles in PubMed

The valosin-containing protein is a novel repressor of cardiomyocyte hypertrophy induced by pressure overload.
Zhou N, Ma B, Stoll S, Hays TT, Qiu H
Aging cell16(5)1168-1179(2017 Oct)

Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3?Ubiquitin Ligase Hyd/UBR5.
Flack JE, Mieszczanek J, Novcic N, Bienz M
Molecular cell67(2)181-193.e5(2017 Jul)

VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H
The EMBO journal36(2)135-150(2017 01)

Valosin-containing protein is a key mediator between autophagic cell death and apoptosis in adult hippocampal neural stem cells following insulin withdrawal.
Yeo BK, Hong CJ, Chung KM, Woo H, Kim K, Jung S, Kim EK, Yu SW
Molecular brain931(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VCP may play an essential role in the initiation of autophagy.
Title: Valosin-containing protein is a key mediator between autophagic cell death and apoptosis in adult hippocampal neural stem cells following insulin withdrawal.

VCP and Jab1 provide a novel signaling pathway for testicular ischemia and may play an important role in ischemia-reperfusion injury induced cell death in rat testis.
Title: Role of p97/Valosin-containing protein (VCP) and Jab1/CSN5 in testicular ischaemia-reperfusion injury.

conclude that the Akt substrate, VCP, mediates the increased expression of iNOS downstream from Hsp22 through an NF-kappaB-dependent mechanism
Title: The valosin-containing protein promotes cardiac survival through the inducible isoform of nitric oxide synthase.

These results suggest that an altered expression pattern of p97/VCP and ubiquitin in the developing rat retina may associate with age-related retinal degeneration.
Title: Expression of p97/VCP and ubiquitin during postnatal development of the degenerating rat retina.

Immunoprecipitation from rat brain shows that neurofibromin forms a complex with VCP and p47. The C-terminal D1D2 region is required.
Title: Valosin-containing protein and neurofibromin interact to regulate dendritic spine density.

The following Vcp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vcp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13227	NM_053864.2 Latest version!	Rattus norvegicus valosin-containing protein (Vcp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vcp ( NM_053864.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446316.1 Rattus norvegicus valosin-containing protein (Vcp), mRNA.

Price & Availability↑

CloneID	ORa13227
Clone ID Related Accession (Same CDS sequence)	NM_053864.2
Accession Version	NM_053864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Rattus norvegicus(Norway rat)
Product	transitional endoplasmic reticulum ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060518.1. On Dec 20, 2003 this sequence version replaced NM_053864.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060518.1, U11760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCTG GAGCCGATTC AAAAGGTGAT GATTTATCAA CAGCCATTCT CAAACAGAAG 
AACCGTCCCA ATCGGTTAAT TGTTGATGAA GCCATCAATG AAGATAACAG TGTGGTGTCC 
TTGTCCCAGC CCAAGATGGA TGAACTACAG TTGTTCAGAG GTGACACGGT GTTGCTAAAA 
GGAAAGAAGA GAAGGGAAGC TGTATGCATT GTTCTTTCTG ATGACACGTG TTCTGATGAG 
AAGATTCGAA TGAATAGAGT TGTTCGGAAT AACCTCCGAG TTCGCCTAGG AGATGTCATC 
AGCATCCAGC CATGCCCTGA TGTAAAGTAT GGCAAACGTA TCCATGTGCT ACCCATTGAT 
GACACAGTGG AAGGCATCAC TGGCAATCTT TTTGAGGTAT ACCTTAAGCC GTACTTCCTG 
GAAGCATATC GGCCCATCCG TAAAGGAGAT ATTTTCCTTG TCCGGGGTGG GATGCGTGCT 
GTGGAGTTCA AAGTAGTAGA GACAGATCCC AGCCCTTACT GTATTGTTGC TCCAGACACA 
GTGATCCACT GTGAGGGGGA GCCAATCAAG CGAGAGGATG AGGAGGAGTC CTTGAATGAA 
GTAGGCTATG ATGACATCGG TGGTTGCAGG AAGCAGCTAG CTCAGATAAA GGAGATGGTG 
GAGCTGCCAC TGAGACATCC TGCACTCTTT AAGGCAATTG GTGTGAAGCC TCCTCGGGGA 
ATCTTGCTAT ATGGACCTCC TGGGACAGGG AAAACCTTGA TTGCCCGAGC TGTGGCAAAT 
GAAACTGGAG CCTTCTTCTT TCTGATCAAT GGTCCTGAAA TCATGAGCAA ATTGGCTGGT 
GAGTCTGAGA GCAACCTTCG TAAAGCCTTT GAGGAAGCTG AAAAGAATGC TCCTGCCATC 
ATCTTCATCG ACGAGCTTGA TGCCATTGCA CCCAAAAGAG AGAAAACTCA CGGGGAAGTG 
GAGCGTCGCA TCGTGTCTCA GTTGTTGACC CTTATGGATG GCCTAAAGCA GAGAGCACAT 
GTGATAGTTA TGGCAGCAAC CAATAGACCC AACAGCATTG ACCCAGCCCT ACGGCGATTT 
GGTCGCTTTG ACAGAGAGGT AGATATTGGA ATCCCTGATG CTACAGGACG TTTGGAAATT 
CTTCAGATCC ATACCAAGAA CATGAAACTG GCAGATGATG TGGACTTGGA ACAGGTAGCC 
AATGAGACTC ATGGTCATGT GGGTGCTGAC TTGGCAGCCC TGTGTTCAGA GGCTGCTCTA 
CAGGCCATCC GGAAAAAAAT GGACCTCATT GACCTAGAAG ATGAGACCAT TGACGCTGAG 
GTCATGAATT CCCTGGCAGT TACTATGGAT GACTTCCGGT GGGCCTTAAG TCAAAGCAAC 
CCATCAGCAC TTCGGGAAAC TGTGGTAGAA GTGCCACAAG TAACCTGGGA AGACATTGGA 
GGCCTGGAGG ATGTCAAACG GGAGCTTCAG GAGTTGGTTC AGTATCCTGT GGAGCATCCA 
GACAAATTCC TCAAATTTGG CATGACTCCT TCCAAAGGTG TTCTTTTCTA TGGACCGCCT 
GGCTGTGGGA AAACCTTACT GGCCAAAGCC ATTGCTAATG AATGCCAGGC TAACTTCATC 
TCCATCAAGG GTCCTGAGCT GCTTACCATG TGGTTTGGGG AATCTGAGGC CAATGTCAGG 
GAAATATTTG ACAAGGCACG ACAAGCTGCC CCCTGTGTAC TCTTCTTTGA TGAGTTAGAT 
TCAATTGCCA AGGCTCGTGG TGGTAATATT GGAGATGGTG GTGGAGCTGC AGACCGAGTC 
ATCAATCAGA TCCTGACAGA AATGGATGGC ATGTCCACAA AAAAGAATGT GTTTATCATT 
GGAGCTACCA ACCGGCCTGA CATCATTGAT CCTGCTATCC TAAGACCTGG CCGTCTTGAT 
CAGCTCATTT ATATCCCACT TCCTGATGAG AAGTCCCGTG TTGCCATCCT AAAAGCCAAT 
CTGCGAAAAT CCCCAGTTGC CAAGGATGTG GATTTGGAGT TCTTGGCTAA GATGACTAAT 
GGCTTTTCTG GAGCTGACCT GACAGAAATT TGCCAACGTG CTTGTAAACT AGCCATTCGT 
GAATCTATCG AGAGTGAGAT TAGGCGAGAA CGAGAGAGGC AGACAAATCC ATCAGCTATG 
GAAGTAGAAG AGGATGATCC AGTGCCTGAG ATCCGCAGAG ATCACTTTGA GGAAGCCATG 
CGTTTTGCCC GACGTTCTGT CAGTGATAAT GACATTCGGA AGTATGAAAT GTTTGCCCAG 
ACACTGCAGC AAAGTCGAGG TTTTGGCAGC TTCAGATTCC CTTCAGGGAA CCAGGGTGGA 
GCTGGCCCAA GTCAGGGCAG TGGAGGTGGC ACAGGTGGCA ATGTGTACAC AGAAGACAAT 
GACGATGACC TCTATGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446316.1
CDS	212..2632
Translation	MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGNVYTEDNDDDLYG

Target ORF information:

RefSeq Version	NM_053864.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus valosin-containing protein (Vcp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053864.2

ORF Insert Sequence:

ATGGCCTCTG GAGCCGATTC AAAAGGTGAT GATTTATCAA CAGCCATTCT CAAACAGAAG 
AACCGTCCCA ATCGGTTAAT TGTTGATGAA GCCATCAATG AAGATAACAG TGTGGTGTCC 
TTGTCCCAGC CCAAGATGGA TGAACTACAG TTGTTCAGAG GTGACACGGT GTTGCTAAAA 
GGAAAGAAGA GAAGGGAAGC TGTATGCATT GTTCTTTCTG ATGACACGTG TTCTGATGAG 
AAGATTCGAA TGAATAGAGT TGTTCGGAAT AACCTCCGAG TTCGCCTAGG AGATGTCATC 
AGCATCCAGC CATGCCCTGA TGTAAAGTAT GGCAAACGTA TCCATGTGCT ACCCATTGAT 
GACACAGTGG AAGGCATCAC TGGCAATCTT TTTGAGGTAT ACCTTAAGCC GTACTTCCTG 
GAAGCATATC GGCCCATCCG TAAAGGAGAT ATTTTCCTTG TCCGGGGTGG GATGCGTGCT 
GTGGAGTTCA AAGTAGTAGA GACAGATCCC AGCCCTTACT GTATTGTTGC TCCAGACACA 
GTGATCCACT GTGAGGGGGA GCCAATCAAG CGAGAGGATG AGGAGGAGTC CTTGAATGAA 
GTAGGCTATG ATGACATCGG TGGTTGCAGG AAGCAGCTAG CTCAGATAAA GGAGATGGTG 
GAGCTGCCAC TGAGACATCC TGCACTCTTT AAGGCAATTG GTGTGAAGCC TCCTCGGGGA 
ATCTTGCTAT ATGGACCTCC TGGGACAGGG AAAACCTTGA TTGCCCGAGC TGTGGCAAAT 
GAAACTGGAG CCTTCTTCTT TCTGATCAAT GGTCCTGAAA TCATGAGCAA ATTGGCTGGT 
GAGTCTGAGA GCAACCTTCG TAAAGCCTTT GAGGAAGCTG AAAAGAATGC TCCTGCCATC 
ATCTTCATCG ACGAGCTTGA TGCCATTGCA CCCAAAAGAG AGAAAACTCA CGGGGAAGTG 
GAGCGTCGCA TCGTGTCTCA GTTGTTGACC CTTATGGATG GCCTAAAGCA GAGAGCACAT 
GTGATAGTTA TGGCAGCAAC CAATAGACCC AACAGCATTG ACCCAGCCCT ACGGCGATTT 
GGTCGCTTTG ACAGAGAGGT AGATATTGGA ATCCCTGATG CTACAGGACG TTTGGAAATT 
CTTCAGATCC ATACCAAGAA CATGAAACTG GCAGATGATG TGGACTTGGA ACAGGTAGCC 
AATGAGACTC ATGGTCATGT GGGTGCTGAC TTGGCAGCCC TGTGTTCAGA GGCTGCTCTA 
CAGGCCATCC GGAAAAAAAT GGACCTCATT GACCTAGAAG ATGAGACCAT TGACGCTGAG 
GTCATGAATT CCCTGGCAGT TACTATGGAT GACTTCCGGT GGGCCTTAAG TCAAAGCAAC 
CCATCAGCAC TTCGGGAAAC TGTGGTAGAA GTGCCACAAG TAACCTGGGA AGACATTGGA 
GGCCTGGAGG ATGTCAAACG GGAGCTTCAG GAGTTGGTTC AGTATCCTGT GGAGCATCCA 
GACAAATTCC TCAAATTTGG CATGACTCCT TCCAAAGGTG TTCTTTTCTA TGGACCGCCT 
GGCTGTGGGA AAACCTTACT GGCCAAAGCC ATTGCTAATG AATGCCAGGC TAACTTCATC 
TCCATCAAGG GTCCTGAGCT GCTTACCATG TGGTTTGGGG AATCTGAGGC CAATGTCAGG 
GAAATATTTG ACAAGGCACG ACAAGCTGCC CCCTGTGTAC TCTTCTTTGA TGAGTTAGAT 
TCAATTGCCA AGGCTCGTGG TGGTAATATT GGAGATGGTG GTGGAGCTGC AGACCGAGTC 
ATCAATCAGA TCCTGACAGA AATGGATGGC ATGTCCACAA AAAAGAATGT GTTTATCATT 
GGAGCTACCA ACCGGCCTGA CATCATTGAT CCTGCTATCC TAAGACCTGG CCGTCTTGAT 
CAGCTCATTT ATATCCCACT TCCTGATGAG AAGTCCCGTG TTGCCATCCT AAAAGCCAAT 
CTGCGAAAAT CCCCAGTTGC CAAGGATGTG GATTTGGAGT TCTTGGCTAA GATGACTAAT 
GGCTTTTCTG GAGCTGACCT GACAGAAATT TGCCAACGTG CTTGTAAACT AGCCATTCGT 
GAATCTATCG AGAGTGAGAT TAGGCGAGAA CGAGAGAGGC AGACAAATCC ATCAGCTATG 
GAAGTAGAAG AGGATGATCC AGTGCCTGAG ATCCGCAGAG ATCACTTTGA GGAAGCCATG 
CGTTTTGCCC GACGTTCTGT CAGTGATAAT GACATTCGGA AGTATGAAAT GTTTGCCCAG 
ACACTGCAGC AAAGTCGAGG TTTTGGCAGC TTCAGATTCC CTTCAGGGAA CCAGGGTGGA 
GCTGGCCCAA GTCAGGGCAG TGGAGGTGGC ACAGGTGGCA ATGTGTACAC AGAAGACAAT 
GACGATGACC TCTATGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The valosin-containing protein is a novel repressor of cardiomyocyte hypertrophy induced by pressure overload.
Aging cell16(5)1168-1179(2017 Oct)
Zhou N,Ma B,Stoll S,Hays TT,Qiu H

Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3?Ubiquitin Ligase Hyd/UBR5.
Molecular cell67(2)181-193.e5(2017 Jul)
Flack JE,Mieszczanek J,Novcic N,Bienz M

VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
The EMBO journal36(2)135-150(2017 01)
Papadopoulos C,Kirchner P,Bug M,Grum D,Koerver L,Schulze N,Poehler R,Dressler A,Fengler S,Arhzaouy K,Lux V,Ehrmann M,Weihl CC,Meyer H

Valosin-containing protein is a key mediator between autophagic cell death and apoptosis in adult hippocampal neural stem cells following insulin withdrawal.
Molecular brain931(2016 Mar)
Yeo BK,Hong CJ,Chung KM,Woo H,Kim K,Jung S,Kim EK,Yu SW