Ccl4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccl4
Entrez Gene ID	116637
Full Name	C-C motif chemokine ligand 4
Synonyms	Mip1-b,Scya4
General protein information	Preferred Names C-C motif chemokine ligand 4 Names C-C motif chemokine 4 MIP-1-beta chemokine (C-C motif) ligand 4 macrophage inflammatory protein 1-beta small-inducible cytokine A4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	a cytokine that is up-regulated during the inflammatory response; may be involved in intrapulmonary recruitment of neutrophils [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053858.1	NP_446310.1	C-C motif chemokine 4 precursor
XM_006246966.3	XP_006247028.1	C-C motif chemokine 4 isoform X1

Pathways from BioSystems

Homologies

	LOC100426632	XP_002800713.1
Canis lupus familiaris (dog)	CCL4	NP_001005250.1
Gallus gallus (chicken)	CCL4	NP_990051.1
	LOC100423861	XP_002800704.1
Mus musculus (house mouse)	Ccl4	NP_038680.1
Pan troglodytes (chimpanzee)	CCL4	XP_001173914.1
	CCL4L1	NP_001028045.1
Bos taurus (cattle)	CCL4	NP_001068615.1
Homo sapiens (human)	CCL4	NP_002975.1
	LOC100430627	XP_002800427.1
Rattus norvegicus (Norway rat)	Ccl4	NP_446310.1

Gene Ontology
Bibliography

Related articles in PubMed

Dihydro-CDDO-trifluoroethyl amide suppresses inflammatory responses in macrophages via activation of Nrf2.
Li B, Abdalrahman A, Lai Y, Janicki JS, Ward KW, Meyer CJ, Wang XL, Tang D, Cui T
Biochemical and biophysical research communications444(4)555-61(2014 Feb)

Signaling lymphocyte activation molecule regulates development of colitis in mice.
van Driel B, Liao G, Romero X, O'Keeffe MS, Wang G, Faubion WA, Berger SB, Magelky EM, Manocha M, Azcutia V, Grisham M, Luscinskas FW, Mizoguchi E, de Waal Malefyt R, Reinecker HC, Bhan AK, Wang N, Terhorst C
Gastroenterology143(6)1544-1554.e7(2012 Dec)

Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1? expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation.
Wang J, Li PT, Du H, Hou JC, Li WH, Pan YS, Chen HC
Journal of ethnopharmacology141(1)151-7(2012 May)

Prolonged increase in rat hippocampal chemokine signalling after status epilepticus.
Kan AA, van der Hel WS, Kolk SM, Bos IW, Verlinde SA, van Nieuwenhuizen O, de Graan PN
Journal of neuroimmunology245(1-2)15-22(2012 Apr)

Serum chemokine profiles in visceral leishmaniasis, HIV and HIV/ visceral leishmaniasis co-infected Ethiopian patients.
Sisay Z, Berhe N, Petros B, Tegbaru B, Messele T, Hailu A, Wolday D
Ethiopian medical journal49(3)179-86(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tong Luo Jiu Nao injection inhibits MIP-1beta expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation and inhibitors microglial cell proliferation.
Title: Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1? expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation.

Early and persistent up-regulation of CCL4/CCR5 signaling during epileptogenesis suggests that CCL4 signaling, rather than CCL2 signaling, could have a role in the epileptogenic process.
Title: Prolonged increase in rat hippocampal chemokine signalling after status epilepticus.

this study we present data on long term increase in CCL4 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy.
Title: Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain.

During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of MIP-1 alpha.
Title: Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.

The following Ccl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10875	NM_053858.1 Latest version!	Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33492	XM_006246966.3 Latest version!	Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccl4 ( NM_053858.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446310.1 Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), mRNA.

Price & Availability↑

CloneID	ORa10875
Clone ID Related Accession (Same CDS sequence)	NM_053858.1
Accession Version	NM_053858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	C-C motif chemokine 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U06434.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF121996.1, EF121997.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTCT GCGTGTCTGC CTTCTCTCTC CTCCTGCTTG TGGCCGCCTT CTGCGATTCA 
GTGCTGTCAG CACCAATAGG CTCTGACCCT CCCACTTCCT GCTGCTTCTC TTACACCTCC 
CGGAAGATTC ATCGGAACTT TGTGATGGAT TACTATGAGA CCAGCAGCCT TTGCTCCCAG 
CCAGCTGTGG TATTTCTGAC CAAAAAAGGC AGGCAAATCT GTGCCGACCC CAGCGAGCCC 
TGGGTCAATG AGTATGTGAA TGACTTGGAG TTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446310.1
CDS	1..279
Translation	MKLCVSAFSLLLLVAAFCDSVLSAPIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN

Target ORF information:

RefSeq Version	NM_053858.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053858.1

ORF Insert Sequence:

ATGAAGCTCT GCGTGTCTGC CTTCTCTCTC CTCCTGCTTG TGGCCGCCTT CTGCGATTCA 
GTGCTGTCAG CACCAATAGG CTCTGACCCT CCCACTTCCT GCTGCTTCTC TTACACCTCC 
CGGAAGATTC ATCGGAACTT TGTGATGGAT TACTATGAGA CCAGCAGCCT TTGCTCCCAG 
CCAGCTGTGG TATTTCTGAC CAAAAAAGGC AGGCAAATCT GTGCCGACCC CAGCGAGCCC 
TGGGTCAATG AGTATGTGAA TGACTTGGAG TTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccl4 ( XM_006246966.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247028.1 Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33492
Clone ID Related Accession (Same CDS sequence)	XM_006246966.3
Accession Version	XM_006246966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	C-C motif chemokine 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCT ACGTGTCTGC CTTCTCTCTC CTCCTGCTTG TGGCCGCCTT CTGCGATTCA 
GTGCTGTCAG CACCAATAGG CTCTGACCCT CCCACTTCCT GCTGCTTCTC TTACACCTCC 
CGGAAGATTC ATCGGAACTT TGTGATGGAT TACTATGAGA CCAGCAGCCT TTGCTCCCAG 
CCAGCTGTGG TATTTCTGAC CAAAAAAGGC AGGCAAATCT GTGCCGACCC CAGCGAGCCC 
TGGGTCAATG AGTATGTGAA TGACTTGGAG TTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247028.1
CDS	204..482
Translation	MKLYVSAFSLLLLVAAFCDSVLSAPIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN

Target ORF information:

RefSeq Version	XM_006246966.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus C-C motif chemokine ligand 4 (Ccl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246966.3

ORF Insert Sequence:

ATGAAGCTCT ACGTGTCTGC CTTCTCTCTC CTCCTGCTTG TGGCCGCCTT CTGCGATTCA 
GTGCTGTCAG CACCAATAGG CTCTGACCCT CCCACTTCCT GCTGCTTCTC TTACACCTCC 
CGGAAGATTC ATCGGAACTT TGTGATGGAT TACTATGAGA CCAGCAGCCT TTGCTCCCAG 
CCAGCTGTGG TATTTCTGAC CAAAAAAGGC AGGCAAATCT GTGCCGACCC CAGCGAGCCC 
TGGGTCAATG AGTATGTGAA TGACTTGGAG TTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dihydro-CDDO-trifluoroethyl amide suppresses inflammatory responses in macrophages via activation of Nrf2.
Biochemical and biophysical research communications444(4)555-61(2014 Feb)
Li B,Abdalrahman A,Lai Y,Janicki JS,Ward KW,Meyer CJ,Wang XL,Tang D,Cui T

Signaling lymphocyte activation molecule regulates development of colitis in mice.
Gastroenterology143(6)1544-1554.e7(2012 Dec)
van Driel B,Liao G,Romero X,O'Keeffe MS,Wang G,Faubion WA,Berger SB,Magelky EM,Manocha M,Azcutia V,Grisham M,Luscinskas FW,Mizoguchi E,de Waal Malefyt R,Reinecker HC,Bhan AK,Wang N,Terhorst C

Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1? expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation.
Journal of ethnopharmacology141(1)151-7(2012 May)
Wang J,Li PT,Du H,Hou JC,Li WH,Pan YS,Chen HC

Prolonged increase in rat hippocampal chemokine signalling after status epilepticus.
Journal of neuroimmunology245(1-2)15-22(2012 Apr)
Kan AA,van der Hel WS,Kolk SM,Bos IW,Verlinde SA,van Nieuwenhuizen O,de Graan PN

Serum chemokine profiles in visceral leishmaniasis, HIV and HIV/ visceral leishmaniasis co-infected Ethiopian patients.
Ethiopian medical journal49(3)179-86(2011 Jul)
Sisay Z,Berhe N,Petros B,Tegbaru B,Messele T,Hailu A,Wolday D