Wbp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wbp4
Entrez Gene ID	114765
Full Name	WW domain binding protein 4
Synonyms	Fbp21
General protein information	Preferred Names WW domain binding protein 4 Names WW domain-binding protein 4 WBP-4 WW domain binding protein 4 (formin binding protein 21) WW domain-containing-binding protein 4 formin-binding protein 21
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15q12
Summary	mouse homolog interacts with several snRNPs and branchpoint binding protein SF1/mBB; may play a role in cross-intron bridging of U1 and U2 snRNPs in spliceosomal complex A [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770840.2	XP_008769062.1	WW domain-binding protein 4 isoform X1
NM_053766.1	NP_446218.1	WW domain-binding protein 4

Pathways from BioSystems

Homologies

	WBP4	XP_001089735.2
Bos taurus (cattle)	WBP4	NP_001068719.1
Drosophila melanogaster (fruit fly)	CG4291	NP_608551.1
Xenopus tropicalis (tropical clawed frog)	wbp4	NP_001039172.1
Homo sapiens (human)	WBP4	NP_009118.1
Pan troglodytes (chimpanzee)	WBP4	XP_509769.2
Rattus norvegicus (Norway rat)	Wbp4	NP_446218.1
Canis lupus familiaris (dog)	WBP4	XP_534136.2
Mus musculus (house mouse)	Wbp4	NP_061235.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010339	XP_311606.4
Gallus gallus (chicken)	WBP4	NP_001025995.1
Danio rerio (zebrafish)	wbp4	NP_001018530.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21).
Huang X, Beullens M, Zhang J, Zhou Y, Nicolaescu E, Lesage B, Hu Q, Wu J, Bollen M, Shi Y
The Journal of biological chemistry284(37)25375-87(2009 Sep)

A systematic analysis of 40 random genes in cultured vascular smooth muscle subtypes reveals a heterogeneity of gene expression and identifies the tight junction gene zonula occludens 2 as a marker of epithelioid "pup" smooth muscle cells and a participant in carotid neointimal formation.
Adams LD, Lemire JM, Schwartz SM
Arteriosclerosis, thrombosis, and vascular biology19(11)2600-8(1999 Nov)

WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif.
Bedford MT, Reed R, Leder P
Proceedings of the National Academy of Sciences of the United States of America95(18)10602-7(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wbp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wbp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10559	NM_053766.1 Latest version!	Rattus norvegicus WW domain binding protein 4 (Wbp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa43959	XM_008770840.2 Latest version!	Rattus norvegicus WW domain binding protein 4 (Wbp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wbp4 ( NM_053766.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446218.1 Rattus norvegicus WW domain binding protein 4 (Wbp4), mRNA.

Price & Availability↑

CloneID	ORa10559
Clone ID Related Accession (Same CDS sequence)	NM_053766.1
Accession Version	NM_053766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	WW domain-binding protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089052.1. On Apr 19, 2005 this sequence version replaced XM_341360.2. ##Evidence-Data-START## Transcript exon combination :: BC089052.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGACT ACTGGAAGTC ACAACCGAAG AAATTCTGTG ATTACTGCAA GTGCTGGATA 
GCCGACAATA GGCCTAGTGT TGAATTTCAT GAAAGAGGAA AGAACCACAA GGAAAATGTG 
GCCAGGAAGA TCAGCGAGAT TAAACAGAAA AGCCTGGATA AGGCAAAGGA AGAAGAAAAG 
GCATCCAAGG AGTTTGCTGC CATGGAGGCG GCTGCCCTGA AGGCATACCA AGAGGATCTG 
AAAAGGCTTG GCTTGCAGTC AGACATTTCA GAGCCAACTA TATCACCAGT CACCAACACT 
GTCCAGCCCA CCCCTACAGC AAATCAACAG AAAGAGAAGA AAAAGAAGAA GAAAAAGAAA 
GAGGCTTCTA AGGGTAGATG GGTGGAAGGC GTAACAGCCG ACGGCCACTG TTACTATTAT 
GATCTCGTCA CAGGAGCATC TCAGTGGGAA AAGCCAGAAG GATTTCAAGG GAACTTAAAA 
AAGACAGCAG CGAAGGCCAT TTGGGTAGAA GGTTTAAGTG AAGATGGCTA CACCTATTAC 
TATAATACAG AAACAGGAGA ATCCAAATGG GAAAAGCCTG ATGATTTCAT TCCACATGGT 
GGTGATGTGC TTTCTAGTAA GGACAGTGAA AAGTTACCTG ACACCCTAGA AGACTCCAAA 
TCATCCGATT CTCACAGCGA TTCTGATGAT GAACAGAAGA AAGCGGGAGA GGCCTCTGCA 
GAAACAAAGA AGCTAATAAT CAAGTTTAAG GAAAAAAATA AAAGTACTGA GAAAAGAATT 
GGCCCAGAAA TACAAAAAGA AAAAACTACT CCAAAACAGA ACCCATCAAA TACAAATGAA 
GAAAAACCCA AAACTCCGAA GAAATCGACA AACCCTTATG GGGAATGGCA AGAAATTAAA 
CAAGAGGCGG AGTCTCAAGA AGAGGTCGAT TTGGAACTTC CAAGCACTGA AAATGAATGT 
CTATCAAGTT CAGAAGCTGG TGCTGGGGAA ATCAAAGTGG TATTTAAAGA GAAAACAGTT 
TCTTCTCTGG GAGTTGCAGC AGATGGAGTG GCCCCAGTCT TCAAAAAGAG AAGAATTGAA 
AATGGAAAAT CTCGAAATTT AAGACAGCGA GGTGAGGACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446218.1
CDS	299..1423
Translation	MADYWKSQPKKFCDYCKCWIADNRPSVEFHERGKNHKENVARKISEIKQKSLDKAKEEEKASKEFAAMEAAALKAYQEDLKRLGLQSDISEPTISPVTNTVQPTPTANQQKEKKKKKKKKEASKGRWVEGVTADGHCYYYDLVTGASQWEKPEGFQGNLKKTAAKAIWVEGLSEDGYTYYYNTETGESKWEKPDDFIPHGGDVLSSKDSEKLPDTLEDSKSSDSHSDSDDEQKKAGEASAETKKLIIKFKEKNKSTEKRIGPEIQKEKTTPKQNPSNTNEEKPKTPKKSTNPYGEWQEIKQEAESQEEVDLELPSTENECLSSSEAGAGEIKVVFKEKTVSSLGVAADGVAPVFKKRRIENGKSRNLRQRGEDE

Target ORF information:

RefSeq Version	NM_053766.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WW domain binding protein 4 (Wbp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053766.1

ORF Insert Sequence:

ATGGCCGACT ACTGGAAGTC ACAACCGAAG AAATTCTGTG ATTACTGCAA GTGCTGGATA 
GCCGACAATA GGCCTAGTGT TGAATTTCAT GAAAGAGGAA AGAACCACAA GGAAAATGTG 
GCCAGGAAGA TCAGCGAGAT TAAACAGAAA AGCCTGGATA AGGCAAAGGA AGAAGAAAAG 
GCATCCAAGG AGTTTGCTGC CATGGAGGCG GCTGCCCTGA AGGCATACCA AGAGGATCTG 
AAAAGGCTTG GCTTGCAGTC AGACATTTCA GAGCCAACTA TATCACCAGT CACCAACACT 
GTCCAGCCCA CCCCTACAGC AAATCAACAG AAAGAGAAGA AAAAGAAGAA GAAAAAGAAA 
GAGGCTTCTA AGGGTAGATG GGTGGAAGGC GTAACAGCCG ACGGCCACTG TTACTATTAT 
GATCTCGTCA CAGGAGCATC TCAGTGGGAA AAGCCAGAAG GATTTCAAGG GAACTTAAAA 
AAGACAGCAG CGAAGGCCAT TTGGGTAGAA GGTTTAAGTG AAGATGGCTA CACCTATTAC 
TATAATACAG AAACAGGAGA ATCCAAATGG GAAAAGCCTG ATGATTTCAT TCCACATGGT 
GGTGATGTGC TTTCTAGTAA GGACAGTGAA AAGTTACCTG ACACCCTAGA AGACTCCAAA 
TCATCCGATT CTCACAGCGA TTCTGATGAT GAACAGAAGA AAGCGGGAGA GGCCTCTGCA 
GAAACAAAGA AGCTAATAAT CAAGTTTAAG GAAAAAAATA AAAGTACTGA GAAAAGAATT 
GGCCCAGAAA TACAAAAAGA AAAAACTACT CCAAAACAGA ACCCATCAAA TACAAATGAA 
GAAAAACCCA AAACTCCGAA GAAATCGACA AACCCTTATG GGGAATGGCA AGAAATTAAA 
CAAGAGGCGG AGTCTCAAGA AGAGGTCGAT TTGGAACTTC CAAGCACTGA AAATGAATGT 
CTATCAAGTT CAGAAGCTGG TGCTGGGGAA ATCAAAGTGG TATTTAAAGA GAAAACAGTT 
TCTTCTCTGG GAGTTGCAGC AGATGGAGTG GCCCCAGTCT TCAAAAAGAG AAGAATTGAA 
AATGGAAAAT CTCGAAATTT AAGACAGCGA GGTGAGGACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wbp4 ( XM_008770840.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769062.1 Rattus norvegicus WW domain binding protein 4 (Wbp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43959
Clone ID Related Accession (Same CDS sequence)	XM_008770840.2
Accession Version	XM_008770840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	WW domain-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTAC AGGAGCATAG ACCCACAAGA GTGTTGAATT TCATGAAAGA GGAAAGAACC 
ACAAGGAAAA TGTGGCCAGG AAGATCAGCG AGGTACCCCT GGCGCTTTCT TCTCTTTCCA 
GGCAATGGTG GTCTCTTCTC TCACTCTTGC CTTCACCTGT GTCTTACACA GATTAAACAG 
AAAAGCCTGG ATAAGGCAAA GGAAGAAGAA AAGGCATCCA AGGAGTTTGC TGCCATGGAG 
GCGGCTGCCC TGAAGGCATA CCAAGAGGAT CTGAAAAGGC TTGGCTTGCA GTCAGACATT 
TCAGAGCCAA CTATATCACC AGTCACCAAC ACTGTCCAGC CCACCCCTAC AGCAAATCAA 
CAGAAAGAGA AGAAAAAGAA GAAGAAAAAG AAAGAGGCTT CTAAGGGTAG ATGGGTGGAA 
GGCGTAACAG CCGACGGCCA CTGTTACTAT TATGATCTCG TCACAGGAGC ATCTCAGTGG 
GAAAAGCCAG AAGGATTTCA AGGGAACTTA AAAAAGACAG CAGCGAAGGC CATTTGGGTA 
GAAGGTTTAA GTGAAGATGG CTACACCTAT TACTATAATA CAGAAACAGG AGAATCCAAA 
TGGGAAAAGC CTGATGATTT CATTCCACAT GGTGGTGATG TGCTTTCTAG TAAGGACAGT 
GAAAAGTTAC CTGACACCCT AGAAGACTCC AAATCATCCG ATTCTCACAG CGATTCTGAT 
GATGAACAGA AGAAAGCGGG AGAGGCCTCT GCAGAAACAA AGAAGCTAAT AATCAAGTTT 
AAGGAAAAAA ATAAAAGTAC TGAGAAAAGA ATTGGCCCAG AAATACAAAA AGAAAAAACT 
ACTCCAAAAC AGAACCCATC AAATACAAAT GAAGAAAAAC CCAAAACTCC GAAGAAATCG 
ACAAACCCTT ATGGGGAATG GCAAGAAATT AAACAAGAGG CGGAGTCTCA AGAAGAGGTC 
GATTTGGAAC TTCCAAGCAC TGAAAATGAA TGTCTATCAA GTTCAGAAGC TGGTGCTGGG 
GAAATCAAAG TGGTATTTAA AGAGAAAACA GTTTCTTCTC TGGGAGTTGC AGCAGATGGA 
GTGGCCCCAG TCTTCAAAAA GAGAAGAATT GAAAATGGAA AATCTCGAAA TTTAAGACAG 
CGAGGTGAGG ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769062.1
CDS	779..1936
Translation	MHLQEHRPTRVLNFMKEERTTRKMWPGRSARYPWRFLLFPGNGGLFSHSCLHLCLTQIKQKSLDKAKEEEKASKEFAAMEAAALKAYQEDLKRLGLQSDISEPTISPVTNTVQPTPTANQQKEKKKKKKKKEASKGRWVEGVTADGHCYYYDLVTGASQWEKPEGFQGNLKKTAAKAIWVEGLSEDGYTYYYNTETGESKWEKPDDFIPHGGDVLSSKDSEKLPDTLEDSKSSDSHSDSDDEQKKAGEASAETKKLIIKFKEKNKSTEKRIGPEIQKEKTTPKQNPSNTNEEKPKTPKKSTNPYGEWQEIKQEAESQEEVDLELPSTENECLSSSEAGAGEIKVVFKEKTVSSLGVAADGVAPVFKKRRIENGKSRNLRQRGEDE

Target ORF information:

RefSeq Version	XM_008770840.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WW domain binding protein 4 (Wbp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770840.2

ORF Insert Sequence:

ATGCACTTAC AGGAGCATAG ACCCACAAGA GTGTTGAATT TCATGAAAGA GGAAAGAACC 
ACAAGGAAAA TGTGGCCAGG AAGATCAGCG AGGTACCCCT GGCGCTTTCT TCTCTTTCCA 
GGCAATGGTG GTCTCTTCTC TCACTCTTGC CTTCACCTGT GTCTTACACA GATTAAACAG 
AAAAGCCTGG ATAAGGCAAA GGAAGAAGAA AAGGCATCCA AGGAGTTTGC TGCCATGGAG 
GCGGCTGCCC TGAAGGCATA CCAAGAGGAT CTGAAAAGGC TTGGCTTGCA GTCAGACATT 
TCAGAGCCAA CTATATCACC AGTCACCAAC ACTGTCCAGC CCACCCCTAC AGCAAATCAA 
CAGAAAGAGA AGAAAAAGAA GAAGAAAAAG AAAGAGGCTT CTAAGGGTAG ATGGGTGGAA 
GGCGTAACAG CCGACGGCCA CTGTTACTAT TATGATCTCG TCACAGGAGC ATCTCAGTGG 
GAAAAGCCAG AAGGATTTCA AGGGAACTTA AAAAAGACAG CAGCGAAGGC CATTTGGGTA 
GAAGGTTTAA GTGAAGATGG CTACACCTAT TACTATAATA CAGAAACAGG AGAATCCAAA 
TGGGAAAAGC CTGATGATTT CATTCCACAT GGTGGTGATG TGCTTTCTAG TAAGGACAGT 
GAAAAGTTAC CTGACACCCT AGAAGACTCC AAATCATCCG ATTCTCACAG CGATTCTGAT 
GATGAACAGA AGAAAGCGGG AGAGGCCTCT GCAGAAACAA AGAAGCTAAT AATCAAGTTT 
AAGGAAAAAA ATAAAAGTAC TGAGAAAAGA ATTGGCCCAG AAATACAAAA AGAAAAAACT 
ACTCCAAAAC AGAACCCATC AAATACAAAT GAAGAAAAAC CCAAAACTCC GAAGAAATCG 
ACAAACCCTT ATGGGGAATG GCAAGAAATT AAACAAGAGG CGGAGTCTCA AGAAGAGGTC 
GATTTGGAAC TTCCAAGCAC TGAAAATGAA TGTCTATCAA GTTCAGAAGC TGGTGCTGGG 
GAAATCAAAG TGGTATTTAA AGAGAAAACA GTTTCTTCTC TGGGAGTTGC AGCAGATGGA 
GTGGCCCCAG TCTTCAAAAA GAGAAGAATT GAAAATGGAA AATCTCGAAA TTTAAGACAG 
CGAGGTGAGG ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21).
The Journal of biological chemistry284(37)25375-87(2009 Sep)
Huang X,Beullens M,Zhang J,Zhou Y,Nicolaescu E,Lesage B,Hu Q,Wu J,Bollen M,Shi Y

A systematic analysis of 40 random genes in cultured vascular smooth muscle subtypes reveals a heterogeneity of gene expression and identifies the tight junction gene zonula occludens 2 as a marker of epithelioid "pup" smooth muscle cells and a participant in carotid neointimal formation.
Arteriosclerosis, thrombosis, and vascular biology19(11)2600-8(1999 Nov)
Adams LD,Lemire JM,Schwartz SM

WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif.
Proceedings of the National Academy of Sciences of the United States of America95(18)10602-7(1998 Sep)
Bedford MT,Reed R,Leder P