Yme1l1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Yme1l1
Entrez Gene ID	114217
Full Name	YME1-like 1 ATPase
Synonyms	FtsH1
General protein information	Preferred Names YME1-like 1 ATPase Names ATP-dependent zinc metalloprotease YME1L1 ATP-dependent metalloprotease FtsH1 homolog ATP-dependent metalloprotease YME1L1 YME1-like protein 1 meg-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	human homolog has similarity to mitochondrial AAA proteases, especially to yeast Yme1p; may be a candidate gene for a form of hereditary spastic paraplegia or neurodegenerative disorder [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053682.2	NP_446134.2	ATP-dependent zinc metalloprotease YME1L1
XM_006254369.3	XP_006254431.1	ATP-dependent zinc metalloprotease YME1L1 isoform X1

Pathways from BioSystems

Homologies

	LOC714687	XP_002805640.1
Canis lupus familiaris (dog)	YME1L1	XP_535172.3
Rattus norvegicus (Norway rat)	Yme1l1	NP_446134.2
Homo sapiens (human)	YME1L1	NP_647473.1
Danio rerio (zebrafish)	yme1l1b	NP_001082983.1
Caenorhabditis elegans (roundworm)	ymel-1	NP_499298.2
Bos taurus (cattle)	YME1L1	NP_001095320.1
Rattus norvegicus (Norway rat)	LOC100910779	XP_003751780.1
Drosophila melanogaster (fruit fly)	CG3499	NP_001261142.1
Xenopus tropicalis (tropical clawed frog)	yme1l1	XP_002933186.2
Pan troglodytes (chimpanzee)	YME1L1	XP_507710.3
Mus musculus (house mouse)	Yme1l1	NP_038799.1
Gallus gallus (chicken)	YME1L1	NP_001026683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009973	XP_319111.4

Gene Ontology
Bibliography

Related articles in PubMed

Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane.
Shi H, Rampello AJ, Glynn SE
Nature communications713301(2016 Oct)

Homozygous YME1L1 mutation causes mitochondriopathy with optic atrophy and mitochondrial network fragmentation.
Hartmann B, Wai T, Hu H, MacVicar T, Musante L, Fischer-Zirnsak B, Stenzel W, Gr?f R, van den Heuvel L, Ropers HH, Wienker TF, H?bner C, Langer T, Kaindl AM
eLife5(2016 08)

YME1L controls the accumulation of respiratory chain subunits and is required for apoptotic resistance, cristae morphogenesis, and cell proliferation.
Stiburek L, Cesnekova J, Kostkova O, Fornuskova D, Vinsova K, Wenchich L, Houstek J, Zeman J
Molecular biology of the cell23(6)1010-23(2012 Mar)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Identification and characterization of YME1L1, a novel paraplegin-related gene.
Coppola M, Pizzigoni A, Banfi S, Bassi MT, Casari G, Incerti B
Genomics66(1)48-54(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Yme1l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Yme1l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12472	NM_053682.2 Latest version!	Rattus norvegicus YME1-like 1 ATPase (Yme1l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa38119	XM_006254369.3 Latest version!	Rattus norvegicus YME1-like 1 ATPase (Yme1l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Yme1l1 ( NM_053682.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446134.2 Rattus norvegicus YME1-like 1 ATPase (Yme1l1), mRNA.

Price & Availability↑

CloneID	ORa12472
Clone ID Related Accession (Same CDS sequence)	NM_053682.2
Accession Version	NM_053682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-09
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent zinc metalloprotease YME1L1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081751.1. On Sep 16, 2004 this sequence version replaced NM_053682.1. ##Evidence-Data-START## Transcript exon combination :: BC081751.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTCTCCC TGTCGAGCAC TGTGCAGCCT CAGGTTACAG TTCCTCTCAG TCATCTCATC 
AATGCCTTCC ATTCACCAAA AAATATATCC GTTTCCGTCA ATACATCTGC TTCTCCAAAA 
CAGCATCGAG ATACAGTTGC TGAGCATGAA GCTCCCAGTA GTGAGCCTGT GCTTAATTTA 
AGGGACCTTG GATTATCTGA ATTGAAAATT GGACAGACTG ATAAACTGGT AGAAAATTTA 
CTTCCTGGAT TTTATAAAGA CAAAAGAGTT TCTTCCTGTT GGCATACATC TCATATCTCG 
GCACAGTCCT TTTTTGAAAA TAAATATGGT CACTTAGATA TGTTCAGTAC ATTACGTTCC 
TCTAGCTTGT ACAGGCAACA TCCAAAAACT CTTCAGAGCA TTTGTTCAGA TCTTCAGAAT 
TTTCCAGTTT TCATACAGTC TCGTGGTTTC AAAACTTTGA AGTCAAGGAC ACGGCGTTTG 
CAATCTACTT CTGAAAGATT AGCAGAAGCA CAGAATATAG CACCATCATT TGTGAAGGGG 
TTTCTTTTGC GGGACAGAGG AACAGATCTT GAGAGTTTGG ACAAACTTAT GAAAACTAAA 
AACATACCTG AAGTTCACCA AGATGCATTT AAAACTGGTT TTGCAGAGGG TTTTCTCAAA 
GCTCAAGCTC TTACACAGAA GACCAATGAT TCCTTAAGGC GAACTCGTCT GATTCTCTTT 
GTTTTGCTCC TGTTTGGCAT TTATGGACTC TTAAAAAATC CATTTTTATC TGTGCGCTTC 
CGGACAACTA CAGGACTTGA TTCTGCAGTA GACCCTGTCC AGATGAAAAA TGTCACTTTT 
GAACATGTTA AAGGGGTGGA GGAGGCCAAA CAAGAATTAC AGGAAGTGGT TGAATTCTTG 
AAAAACCCAC AGAAGTTTAC TGTGCTTGGA GGTAAACTTC CCAAAGGAAT TCTCTTAGTG 
GGGCCACCAG GAACAGGGAA GACCCTTCTT GCCCGAGCTG TGGCAGGAGA AGCTGATGTT 
CCTTTTTATT ATGCTTCTGG ATCAGAATTC GATGAGATGT TTGTGGGTGT AGGAGCCAGC 
AGGATCAGAA ATCTTTTTAG AGAAGCAAAA GCAAATGCTC CTTGTGTTAT ATTCATTGAT 
GAATTGGATT CTGTTGGTGG GAAGAGAATT GAATCTCCAA TGCATCCATA TTCAAGGCAG 
ACTATCAATC AACTTCTTGC CGAAATGGAT GGTTTCAAAC CTAATGAAGG AGTAATCATT 
ATAGGTGCCA CAAATTTCCC AGAGGCATTA GATAATGCCT TAATACGTCC TGGTCGTTTT 
GATATGCAAG TTACAGTTCC AAGGCCAGAT GTGAAGGGTC GTACTGAAAT TTTGAAATGG 
TATCTTAATA AAATAAAGTT TGATAAATCT GTTGATCCAG AAATCATAGC TCGAGGAACT 
GTTGGGTTTT CTGGAGCAGA GTTGGAGAAT CTTGTGAACC AAGCTGCATT AAAGGCAGCA 
GTTGATGGAA AAGAAATGGT TACCATGAAG GAACTAGAGT TTTCCAAGGA TAAAATTCTA 
ATGGGACCAG AAAGAAGAAG TGTGGAAATT GATAACAAAA ACAAAACTAT AACAGCCTAT 
CATGAATCTG GTCATGCTAT TATTGCATAT TACACAAAGG ATGCAATGCC AATCAATAAA 
GCTACAATCA TGCCACGAGG GCCCACACTT GGACATGTAT CACTGTTGCC TGAGAATGAC 
AGATGGAATG AAACTAGAGC CCAGCTGCTT GCACAGATGG ATGTTAGTAT GGGAGGAAGA 
GTGGCAGAGG AACTCATATT TGGAACCGAT CATATTACAA CTGGTGCTTC TAGTGATTTT 
GATAATGCAA CAAAAATTGC AAAGAGGATG GTTACCAAAT TTGGAATGAG TGAAAAGCTT 
GGAGTTATGA CCTACAGTGA TACAGGAAAG CTAAGTCCTG AAACTCAATC GGCCATTGAA 
CAAGAAATAA GAATCCTTCT ACGGGAATCA TATGAACGAG CAAAACATAT CCTGAAAACA 
CATGCGAAAG AACATAAGAA CCTGGCCGAG GCGTTGCTGA CCTATGAGAC TTTGGATGCC 
AAAGAGATTC AAATTGTTCT TGAGGGGAAG AAATTAGAAG TGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446134.2
CDS	167..2314
Translation	MFSLSSTVQPQVTVPLSHLINAFHSPKNISVSVNTSASPKQHRDTVAEHEAPSSEPVLNLRDLGLSELKIGQTDKLVENLLPGFYKDKRVSSCWHTSHISAQSFFENKYGHLDMFSTLRSSSLYRQHPKTLQSICSDLQNFPVFIQSRGFKTLKSRTRRLQSTSERLAEAQNIAPSFVKGFLLRDRGTDLESLDKLMKTKNIPEVHQDAFKTGFAEGFLKAQALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTFEHVKGVEEAKQELQEVVEFLKNPQKFTVLGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDKSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRESYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR

Target ORF information:

RefSeq Version	NM_053682.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus YME1-like 1 ATPase (Yme1l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053682.2

ORF Insert Sequence:

ATGTTCTCCC TGTCGAGCAC TGTGCAGCCT CAGGTTACAG TTCCTCTCAG TCATCTCATC 
AATGCCTTCC ATTCACCAAA AAATATATCC GTTTCCGTCA ATACATCTGC TTCTCCAAAA 
CAGCATCGAG ATACAGTTGC TGAGCATGAA GCTCCCAGTA GTGAGCCTGT GCTTAATTTA 
AGGGACCTTG GATTATCTGA ATTGAAAATT GGACAGACTG ATAAACTGGT AGAAAATTTA 
CTTCCTGGAT TTTATAAAGA CAAAAGAGTT TCTTCCTGTT GGCATACATC TCATATCTCG 
GCACAGTCCT TTTTTGAAAA TAAATATGGT CACTTAGATA TGTTCAGTAC ATTACGTTCC 
TCTAGCTTGT ACAGGCAACA TCCAAAAACT CTTCAGAGCA TTTGTTCAGA TCTTCAGAAT 
TTTCCAGTTT TCATACAGTC TCGTGGTTTC AAAACTTTGA AGTCAAGGAC ACGGCGTTTG 
CAATCTACTT CTGAAAGATT AGCAGAAGCA CAGAATATAG CACCATCATT TGTGAAGGGG 
TTTCTTTTGC GGGACAGAGG AACAGATCTT GAGAGTTTGG ACAAACTTAT GAAAACTAAA 
AACATACCTG AAGTTCACCA AGATGCATTT AAAACTGGTT TTGCAGAGGG TTTTCTCAAA 
GCTCAAGCTC TTACACAGAA GACCAATGAT TCCTTAAGGC GAACTCGTCT GATTCTCTTT 
GTTTTGCTCC TGTTTGGCAT TTATGGACTC TTAAAAAATC CATTTTTATC TGTGCGCTTC 
CGGACAACTA CAGGACTTGA TTCTGCAGTA GACCCTGTCC AGATGAAAAA TGTCACTTTT 
GAACATGTTA AAGGGGTGGA GGAGGCCAAA CAAGAATTAC AGGAAGTGGT TGAATTCTTG 
AAAAACCCAC AGAAGTTTAC TGTGCTTGGA GGTAAACTTC CCAAAGGAAT TCTCTTAGTG 
GGGCCACCAG GAACAGGGAA GACCCTTCTT GCCCGAGCTG TGGCAGGAGA AGCTGATGTT 
CCTTTTTATT ATGCTTCTGG ATCAGAATTC GATGAGATGT TTGTGGGTGT AGGAGCCAGC 
AGGATCAGAA ATCTTTTTAG AGAAGCAAAA GCAAATGCTC CTTGTGTTAT ATTCATTGAT 
GAATTGGATT CTGTTGGTGG GAAGAGAATT GAATCTCCAA TGCATCCATA TTCAAGGCAG 
ACTATCAATC AACTTCTTGC CGAAATGGAT GGTTTCAAAC CTAATGAAGG AGTAATCATT 
ATAGGTGCCA CAAATTTCCC AGAGGCATTA GATAATGCCT TAATACGTCC TGGTCGTTTT 
GATATGCAAG TTACAGTTCC AAGGCCAGAT GTGAAGGGTC GTACTGAAAT TTTGAAATGG 
TATCTTAATA AAATAAAGTT TGATAAATCT GTTGATCCAG AAATCATAGC TCGAGGAACT 
GTTGGGTTTT CTGGAGCAGA GTTGGAGAAT CTTGTGAACC AAGCTGCATT AAAGGCAGCA 
GTTGATGGAA AAGAAATGGT TACCATGAAG GAACTAGAGT TTTCCAAGGA TAAAATTCTA 
ATGGGACCAG AAAGAAGAAG TGTGGAAATT GATAACAAAA ACAAAACTAT AACAGCCTAT 
CATGAATCTG GTCATGCTAT TATTGCATAT TACACAAAGG ATGCAATGCC AATCAATAAA 
GCTACAATCA TGCCACGAGG GCCCACACTT GGACATGTAT CACTGTTGCC TGAGAATGAC 
AGATGGAATG AAACTAGAGC CCAGCTGCTT GCACAGATGG ATGTTAGTAT GGGAGGAAGA 
GTGGCAGAGG AACTCATATT TGGAACCGAT CATATTACAA CTGGTGCTTC TAGTGATTTT 
GATAATGCAA CAAAAATTGC AAAGAGGATG GTTACCAAAT TTGGAATGAG TGAAAAGCTT 
GGAGTTATGA CCTACAGTGA TACAGGAAAG CTAAGTCCTG AAACTCAATC GGCCATTGAA 
CAAGAAATAA GAATCCTTCT ACGGGAATCA TATGAACGAG CAAAACATAT CCTGAAAACA 
CATGCGAAAG AACATAAGAA CCTGGCCGAG GCGTTGCTGA CCTATGAGAC TTTGGATGCC 
AAAGAGATTC AAATTGTTCT TGAGGGGAAG AAATTAGAAG TGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Yme1l1 ( XM_006254369.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254431.1 Rattus norvegicus YME1-like 1 ATPase (Yme1l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38119
Clone ID Related Accession (Same CDS sequence)	XM_006254369.3
Accession Version	XM_006254369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent zinc metalloprotease YME1L1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGAA CCTGGGTTAC AGTTCCTCTC AGTCATCTCA TCAATGCCTT CCATTCACCA 
AAAAATATAT CCGTTTCCGT CAATACATCT GCTTCTCCAA AACAGCATCG AGATACAGTT 
GCTGAGCATG AAGCTCCCAG TAGTGAGCCT GTGCTTAATT TAAGGGACCT TGGATTATCT 
GAATTGAAAA TTGGACAGAT TGATAAACTG GTAGAAAATT TACTTCCTGG ATTTTATAAA 
GACAAAAGAG TTTCTTCCTG TTGGCATACA TCTCATATCT CGGCACAGTC CTTTTTTGAA 
AATAAATATG GTCACTTAGA TATGTTCAGT ACATTACGTT CCTCTAGCTT GTACAGGCAA 
CATCCAAAAA CTCTTCAGAG CATTTGTTCA GATCTTCAGA ATTTTCCAGT TTTCATACAG 
TCTCGTGGTT TCAAAACTTT GAAGTCAAGG ACACGGCGTT TGCAATCTAC TTCTGAAAGA 
TTAGCAGAAG CACAGAATAT AGCACCATCA TTTGTGAAGG GGTTTCTTTT GCGGGACAGA 
GGAACAGATC TTGAGAGTTT GGACAAACTT ATGAAAACTA AAAACATACC TGAAGCTCAC 
CAAGATGCAT TTAAAACTGG TTTTGCAGAG GGTTTTCTCA AAGCTCAAGC TCTTACACAG 
AAGACCAATG ATTCCTTAAG GCGAACTCGT CTGATTCTCT TTGTTTTGCT CCTGTTTGGC 
ATTTATGGAC TCTTAAAAAA TCCATTTTTA TCTGTGCGCT TCCGGACAAC TACAGGACTT 
GATTCTGCAG TAGACCCTGT CCAGATGAAA AATGTCACTT TTGAACATGT TAAAGGGGTG 
GAGGAGGCCA AACAAGAATT ACAGGAAGTG GTTGAATTCT TGAAAAACCC ACAGAAGTTT 
ACTGTGCTTG GAGGTAAACT TCCCAAAGGA ATTCTCTTAG TGGGGCCACC AGGAACAGGG 
AAGACCCTTC TTGCCCGAGC TGTGGCAGGA GAAGCTGATG TTCCTTTTTA TTATGCTTCT 
GGATCAGAAT TCGATGAGAT GTTTGTGGGT GTAGGAGCCA GCAGGATCAG AAATCTTTTT 
AGAGAAGCAA AAGCAAATGC TCCTTGTGTT ATATTCATTG ATGAATTGGA TTCTGTTGGT 
GGGAAGAGAA TTGAATCTCC AATGCATCCA TATTCAAGGC AGACTATCAA TCAACTTCTT 
GCCGAAATGG ATGGTTTCAA ACCTAATGAA GGAGTAATCA TTATAGGTGC CACAAATTTC 
CCAGAGGCAT TAGATAATGC CTTAATACGT CCTGGTCGTT TTGATATGCA AGTTACAGTT 
CCAAGGCCAG ATGTGAAGGG TCGTACTGAA ATTTTGAAAT GGTATCTTAA TAAAATAAAG 
TTTGATAAAT CTGTTGATCC AGAAATCATA GCTCGAGGAA CTGTTGGGTT TTCTGGAGCA 
GAGTTGGAGA ATCTTGTGAA CCAAGCTGCA TTAAAGGCAG CAGTTGATGG AAAAGAAATG 
GTTACCATGA AGGAACTAGA GTTTTCCAAG GATAAAATTC TAATGGGACC AGAAAGAAGA 
AGTGTGGAAA TTGATAACAA AAACAAAACT ATAACAGCCT ATCATGAATC TGGTCATGCT 
ATTATTGCAT ATTACACAAA GGATGCAATG CCAATCAATA AAGCTACAAT CATGCCACGA 
GGGCCCACAC TTGGACATGT ATCACTGTTG CCTGAGAATG ACAGATGGAA TGAAACTAGA 
GCCCAGCTGC TTGCACAGAT GGATGTTAGT ATGGGAGGAA GAGTGGCAGA GGAACTCATA 
TTTGGAACCG ATCATATTAC AACTGGTGCT TCTAGTGATT TTGATAATGC AACAAAAATT 
GCAAAGAGGA TGGTTACCAA ATTTGGAATG AGTGAAAAGC TTGGAGTTAT GACCTACAGT 
GATACAGGAA AGCTAAGTCC TGAAACTCAA TCGGCCATTG AACAAGAAAT AAGAATCCTT 
CTACGGGAAT CATATGAACG AGCAAAACAT ATCCTGAAAA CACATGCGAA AGAACATAAG 
AACCTGGCCG AGGCGTTGCT GACCTATGAG ACTTTGGATG CCAAAGAGAT TCAAATTGTT 
CTTGAGGGGA AGAAATTAGA AGTGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254431.1
CDS	172..2301
Translation	MWRTWVTVPLSHLINAFHSPKNISVSVNTSASPKQHRDTVAEHEAPSSEPVLNLRDLGLSELKIGQIDKLVENLLPGFYKDKRVSSCWHTSHISAQSFFENKYGHLDMFSTLRSSSLYRQHPKTLQSICSDLQNFPVFIQSRGFKTLKSRTRRLQSTSERLAEAQNIAPSFVKGFLLRDRGTDLESLDKLMKTKNIPEAHQDAFKTGFAEGFLKAQALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTFEHVKGVEEAKQELQEVVEFLKNPQKFTVLGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDKSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRESYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR

Target ORF information:

RefSeq Version	XM_006254369.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus YME1-like 1 ATPase (Yme1l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254369.3

ORF Insert Sequence:

ATGTGGAGAA CCTGGGTTAC AGTTCCTCTC AGTCATCTCA TCAATGCCTT CCATTCACCA 
AAAAATATAT CCGTTTCCGT CAATACATCT GCTTCTCCAA AACAGCATCG AGATACAGTT 
GCTGAGCATG AAGCTCCCAG TAGTGAGCCT GTGCTTAATT TAAGGGACCT TGGATTATCT 
GAATTGAAAA TTGGACAGAT TGATAAACTG GTAGAAAATT TACTTCCTGG ATTTTATAAA 
GACAAAAGAG TTTCTTCCTG TTGGCATACA TCTCATATCT CGGCACAGTC CTTTTTTGAA 
AATAAATATG GTCACTTAGA TATGTTCAGT ACATTACGTT CCTCTAGCTT GTACAGGCAA 
CATCCAAAAA CTCTTCAGAG CATTTGTTCA GATCTTCAGA ATTTTCCAGT TTTCATACAG 
TCTCGTGGTT TCAAAACTTT GAAGTCAAGG ACACGGCGTT TGCAATCTAC TTCTGAAAGA 
TTAGCAGAAG CACAGAATAT AGCACCATCA TTTGTGAAGG GGTTTCTTTT GCGGGACAGA 
GGAACAGATC TTGAGAGTTT GGACAAACTT ATGAAAACTA AAAACATACC TGAAGCTCAC 
CAAGATGCAT TTAAAACTGG TTTTGCAGAG GGTTTTCTCA AAGCTCAAGC TCTTACACAG 
AAGACCAATG ATTCCTTAAG GCGAACTCGT CTGATTCTCT TTGTTTTGCT CCTGTTTGGC 
ATTTATGGAC TCTTAAAAAA TCCATTTTTA TCTGTGCGCT TCCGGACAAC TACAGGACTT 
GATTCTGCAG TAGACCCTGT CCAGATGAAA AATGTCACTT TTGAACATGT TAAAGGGGTG 
GAGGAGGCCA AACAAGAATT ACAGGAAGTG GTTGAATTCT TGAAAAACCC ACAGAAGTTT 
ACTGTGCTTG GAGGTAAACT TCCCAAAGGA ATTCTCTTAG TGGGGCCACC AGGAACAGGG 
AAGACCCTTC TTGCCCGAGC TGTGGCAGGA GAAGCTGATG TTCCTTTTTA TTATGCTTCT 
GGATCAGAAT TCGATGAGAT GTTTGTGGGT GTAGGAGCCA GCAGGATCAG AAATCTTTTT 
AGAGAAGCAA AAGCAAATGC TCCTTGTGTT ATATTCATTG ATGAATTGGA TTCTGTTGGT 
GGGAAGAGAA TTGAATCTCC AATGCATCCA TATTCAAGGC AGACTATCAA TCAACTTCTT 
GCCGAAATGG ATGGTTTCAA ACCTAATGAA GGAGTAATCA TTATAGGTGC CACAAATTTC 
CCAGAGGCAT TAGATAATGC CTTAATACGT CCTGGTCGTT TTGATATGCA AGTTACAGTT 
CCAAGGCCAG ATGTGAAGGG TCGTACTGAA ATTTTGAAAT GGTATCTTAA TAAAATAAAG 
TTTGATAAAT CTGTTGATCC AGAAATCATA GCTCGAGGAA CTGTTGGGTT TTCTGGAGCA 
GAGTTGGAGA ATCTTGTGAA CCAAGCTGCA TTAAAGGCAG CAGTTGATGG AAAAGAAATG 
GTTACCATGA AGGAACTAGA GTTTTCCAAG GATAAAATTC TAATGGGACC AGAAAGAAGA 
AGTGTGGAAA TTGATAACAA AAACAAAACT ATAACAGCCT ATCATGAATC TGGTCATGCT 
ATTATTGCAT ATTACACAAA GGATGCAATG CCAATCAATA AAGCTACAAT CATGCCACGA 
GGGCCCACAC TTGGACATGT ATCACTGTTG CCTGAGAATG ACAGATGGAA TGAAACTAGA 
GCCCAGCTGC TTGCACAGAT GGATGTTAGT ATGGGAGGAA GAGTGGCAGA GGAACTCATA 
TTTGGAACCG ATCATATTAC AACTGGTGCT TCTAGTGATT TTGATAATGC AACAAAAATT 
GCAAAGAGGA TGGTTACCAA ATTTGGAATG AGTGAAAAGC TTGGAGTTAT GACCTACAGT 
GATACAGGAA AGCTAAGTCC TGAAACTCAA TCGGCCATTG AACAAGAAAT AAGAATCCTT 
CTACGGGAAT CATATGAACG AGCAAAACAT ATCCTGAAAA CACATGCGAA AGAACATAAG 
AACCTGGCCG AGGCGTTGCT GACCTATGAG ACTTTGGATG CCAAAGAGAT TCAAATTGTT 
CTTGAGGGGA AGAAATTAGA AGTGAGATGA