C5ar1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	C5ar1
Entrez Gene ID	113959
Full Name	complement C5a receptor 1
Synonyms	C5r1
General protein information	Preferred Names complement C5a receptor 1 Names C5a anaphylatoxin chemotactic receptor 1 C5a-R C5aR complement component 5, receptor 1 complement component 5a receptor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	binds complement component 5 and induces Ca+2 release from intracellular stores [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053619.1	NP_446071.1	C5a anaphylatoxin chemotactic receptor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	C5AR1	NP_001727.1
Mus musculus (house mouse)	C5ar1	NP_001167021.1
Rattus norvegicus (Norway rat)	C5ar1	NP_446071.1
	C5AR1	XP_001110274.2
Pan troglodytes (chimpanzee)	C5AR1	XP_524316.3
Bos taurus (cattle)	C5AR1	NP_001007811.2
Xenopus tropicalis (tropical clawed frog)	c5ar1	XP_002941328.1
Canis lupus familiaris (dog)	C5AR1	NP_001003373.1
Gallus gallus (chicken)	C5AR1	XP_004950957.1

Gene Ontology
Bibliography

Related articles in PubMed

Schisantherin A attenuates ischemia/reperfusion-induced neuronal injury in rats via regulation of TLR4 and C5aR1 signaling pathways.
Shi YW, Zhang XC, Chen C, Tang M, Wang ZW, Liang XM, Ding F, Wang CP
Brain, behavior, and immunity66244-256(2017 Nov)

Expression of C5a and its receptor following spinal cord ischemia reperfusion injury in the rat.
Dong Q, Sun L, Peng L, Yan B, Lv J, Wang G, Gong S
Spinal cord53(8)581-4(2015 Aug)

Novel insights in localization and expression levels of C5aR and C5L2 under native and post-transplant conditions in the kidney.
van Werkhoven MB, Damman J, Daha MR, Krikke C, van Goor H, van Son WJ, Hillebrands JL, van Dijk MC, Seelen MA
Molecular immunology53(3)237-45(2013 Mar)

C5aR and C3aR antagonists each inhibit diet-induced obesity, metabolic dysfunction, and adipocyte and macrophage signaling.
Lim J, Iyer A, Suen JY, Seow V, Reid RC, Brown L, Fairlie DP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(2)822-31(2013 Feb)

Human colonic epithelial cells detect and respond to C5a via apically expressed C5aR through the ERK pathway.
Cao Q, McIsaac SM, Stadnyk AW
American journal of physiology. Cell physiology302(12)C1731-40(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The C5aR expression was upregulated and localized to neurons and microglia in spinal cord ischemia reperfusion injury in the rat.
Title: Expression of C5a and its receptor following spinal cord ischemia reperfusion injury in the rat.

C5aR and C5L2, but not C3aR, are expressed on CD11b-positive leukocytes in islet preparations
Title: Expression of receptors for anaphylatoxins C3a and C5a on rat islet preparations.

C5aR is critically involved in ventricular remodeling by regulating inflammatory responses in the hypertensive heart.
Title: Inhibition of inflammation and fibrosis by a complement C5a receptor antagonist in DOCA-salt hypertensive rats.

present in bone forming osteoblasts and bone resorbing osteoclasts, suggesting role in fracture healing
Title: The anaphylatoxin receptor C5aR is present during fracture healing in rats and mediates osteoblast migration in vitro.

These results demonstrate that CD88 is expressed on presynaptic terminals of mossy fibres within the CA3 region of the hippocampus.
Title: The C5a anaphylatoxin receptor CD88 is expressed in presynaptic terminals of hippocampal mossy fibres.

The following C5ar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C5ar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10101	NM_053619.1 Latest version!	Rattus norvegicus complement C5a receptor 1 (C5ar1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C5ar1 ( NM_053619.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446071.1 Rattus norvegicus complement C5a receptor 1 (C5ar1), mRNA.

Price & Availability↑

CloneID	ORa10101
Clone ID Related Accession (Same CDS sequence)	NM_053619.1
Accession Version	NM_053619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	C5a anaphylatoxin chemotactic receptor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB003042.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB003042.1, BC078770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCCA TAAGTAACGA CAGCAGTGAA ATCACCTATG ATTACTCTGA TGGAACCCCA 
AATCCTGACA TGCCTGCAGA TGGCGTTTAC ATTCCGAAGA TGGAACCTGG GGACATTGCA 
GCCCTTATTA TCTACTTGGC CGTGTTCCTG GTAGGAGTTA CCGGAAATGC CCTGGTGGTG 
TGGGTGACAG CCTTTGAGGC CAAACGTACT GTCAACGCCA TTTGGTTTCT GAATCTGGCG 
GTGGCCGACC TCCTCTCGTG CTTGGCACTG CCTATCCTGT TTACGTCCAT TGTAAAGCAT 
AACCACTGGC CCTTCGGTGA CCAGGCCTGT ATAGTCCTGC CCTCGCTCAT TCTGCTCAAC 
ATGTACTCCA GCATCCTGCT GCTGGCCACT ATTAGTGCCG ACCGTTTCCT GCTGGTGTTC 
AAACCCATCT GGTGTCAGAA GTTCCGCCGG CCTGGCCTGG CCTGGATGGC TTGCGGAGTA 
ACCTGGGTCT TAGCATTGCT CCTCACCATT CCGTCCTTCG TGTTCCGGCG GATACATAAG 
GACCCCTACT CAGATAGCAT TCTATGTAAT ATTGACTATA GTAAGGGTCC GTTCTTCATA 
GAGAAGGCTA TAGCCATCCT GCGGCTGATG GTGGGTTTCG TGTTGCCTCT GCTCACTCTT 
AACATCTGCT ACACCTTCCT CCTGATCCGG ACCTGGAGTC GCAAGGCCAC GCGCTCCACC 
AAGACGCTCA AAGTGGTGAT GGCGGTGGTC ACATGTTTCT TTGTCTTCTG GCTACCCTAC 
CAGGTAACAG GGGTGATATT AGCTTGGCTG CCCCGGTCCT CATCCACTTT TCAGTCAGTG 
GAGAGGCTGA ACTCCTTGTG CGTTTCCCTG GCCTACATCA ACTGCTGTGT TAACCCTATC 
ATCTACGTCA TGGCTGGCCA GGGTTTCCAT GGGAGACTCC GAAGGTCACT CCCCAGCATC 
ATACGAAACG TGCTCTCTGA GGACTCCTTG GGCAGGGACA GCAAGAGTTT CACTCGGTCC 
ACGATGGATA CCTCCACCCA GAAGAGTCAG GCAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446071.1
CDS	44..1102
Translation	MDPISNDSSEITYDYSDGTPNPDMPADGVYIPKMEPGDIAALIIYLAVFLVGVTGNALVVWVTAFEAKRTVNAIWFLNLAVADLLSCLALPILFTSIVKHNHWPFGDQACIVLPSLILLNMYSSILLLATISADRFLLVFKPIWCQKFRRPGLAWMACGVTWVLALLLTIPSFVFRRIHKDPYSDSILCNIDYSKGPFFIEKAIAILRLMVGFVLPLLTLNICYTFLLIRTWSRKATRSTKTLKVVMAVVTCFFVFWLPYQVTGVILAWLPRSSSTFQSVERLNSLCVSLAYINCCVNPIIYVMAGQGFHGRLRRSLPSIIRNVLSEDSLGRDSKSFTRSTMDTSTQKSQAV

Target ORF information:

RefSeq Version	NM_053619.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complement C5a receptor 1 (C5ar1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053619.1

ORF Insert Sequence:

ATGGACCCCA TAAGTAACGA CAGCAGTGAA ATCACCTATG ATTACTCTGA TGGAACCCCA 
AATCCTGACA TGCCTGCAGA TGGCGTTTAC ATTCCGAAGA TGGAACCTGG GGACATTGCA 
GCCCTTATTA TCTACTTGGC CGTGTTCCTG GTAGGAGTTA CCGGAAATGC CCTGGTGGTG 
TGGGTGACAG CCTTTGAGGC CAAACGTACT GTCAACGCCA TTTGGTTTCT GAATCTGGCG 
GTGGCCGACC TCCTCTCGTG CTTGGCACTG CCTATCCTGT TTACGTCCAT TGTAAAGCAT 
AACCACTGGC CCTTCGGTGA CCAGGCCTGT ATAGTCCTGC CCTCGCTCAT TCTGCTCAAC 
ATGTACTCCA GCATCCTGCT GCTGGCCACT ATTAGTGCCG ACCGTTTCCT GCTGGTGTTC 
AAACCCATCT GGTGTCAGAA GTTCCGCCGG CCTGGCCTGG CCTGGATGGC TTGCGGAGTA 
ACCTGGGTCT TAGCATTGCT CCTCACCATT CCGTCCTTCG TGTTCCGGCG GATACATAAG 
GACCCCTACT CAGATAGCAT TCTATGTAAT ATTGACTATA GTAAGGGTCC GTTCTTCATA 
GAGAAGGCTA TAGCCATCCT GCGGCTGATG GTGGGTTTCG TGTTGCCTCT GCTCACTCTT 
AACATCTGCT ACACCTTCCT CCTGATCCGG ACCTGGAGTC GCAAGGCCAC GCGCTCCACC 
AAGACGCTCA AAGTGGTGAT GGCGGTGGTC ACATGTTTCT TTGTCTTCTG GCTACCCTAC 
CAGGTAACAG GGGTGATATT AGCTTGGCTG CCCCGGTCCT CATCCACTTT TCAGTCAGTG 
GAGAGGCTGA ACTCCTTGTG CGTTTCCCTG GCCTACATCA ACTGCTGTGT TAACCCTATC 
ATCTACGTCA TGGCTGGCCA GGGTTTCCAT GGGAGACTCC GAAGGTCACT CCCCAGCATC 
ATACGAAACG TGCTCTCTGA GGACTCCTTG GGCAGGGACA GCAAGAGTTT CACTCGGTCC 
ACGATGGATA CCTCCACCCA GAAGAGTCAG GCAGTGTAG