VPS54 cDNA ORF clone, Python bivittatus(Burmese python)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS54
Entrez Gene ID	103059199
Full Name	VPS54, GARP complex subunit
Gene Type	protein-coding
Organism	Python bivittatus(Burmese python)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007435755.2	XP_007435817.1	vacuolar protein sorting-associated protein 54
XM_007435755.1	XP_007435817.1	vacuolar protein sorting-associated protein 54

Pathways from BioSystems
Gene Ontology

The following VPS54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPy13424	XM_007435755.2 Latest version!	Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OPy13424	XM_007435755.1	Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS54 ( XM_007435755.2 ) cDNA ORF clone, Python bivittatus(Burmese python) -> XP_007435817.1 Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.

Price & Availability↑

CloneID	OPy13424
Clone ID Related Accession (Same CDS sequence)	XM_007435755.2 , XM_007435755.1
Accession Version	XM_007435755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Python bivittatus(Burmese python)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006535008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 24, 2018 this sequence version replaced XM_007435755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Python bivittatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA GCCACCGGTC TTCACCAGTG CCTCAGTCAA AGAACAATGA TGCTTTCTTT 
AAAAAGGAAA TGGATTCTTC CAAATGCTTT CGACCAGTGC GCACACTACC AGATGTATGT 
CCCAAGGAAC CCACAGGTGA TCCTAATAGC TTATGTGACA GCCCATCTTT AGTTGTTGAT 
CAGCATCGAT GGACTATTTA TCATTCCAAA GTAAATCTCC CAGCAGCACT AAATGATCCT 
CGATTAACAA AAAGGGAATC TGATTTCTTT ACAAAAACAT GGGGACAGGA TTTTGTGGAC 
ATTGAAATCG TGCCTTCGCT CTACCTCCCA CAGATCAACA AGGAACATTT TGTAACATAC 
CAACAGGAAG TTGCTCAGAA AGAGAAGATT CATGAGAAAT GTAAGAGTAT TTGTCCTCCT 
AAAGATACTT TTGACAGGAC ATTGTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAA 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATCCCGT AACATTTAAT 
GCAGTATTAC CTTGGTCTCA TTTTAATACT GCTTCTGGGA AAGGAAACCG TGATGCAGCT 
TCCTCCAAGT TACTTCAAGA AAAGCTGAGC CACTACCTGG ATATTGTGGA AGTAAATATT 
GCTCATCAGA TCTCTCTCCG ATCAGAAGCT TTTTTTCATG CAATGACCTC TCAGCATGAA 
CTGCAAGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATAGGC 
CAAATAGATA AAGTCATGTG CGAAGGATCC CTTAAAGTGT TAAGTTTATC ACTGAGTAGA 
AATAACTGTA TAAAAGCATA TAATAAGCTG AGGTTGATGG CAACTGTACA CCAAACACAA 
CCTACAGTAC AGCTATTGTT GTCCACTTCT GAATTTGTTG GAGCACTAGA TTTAATTGCA 
ACAACACAGG AAGTGCTACA GCAGGAACTT CAGGGCATAC ACAGTTTCCG GCATCTTGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA GTTCTCTTCT 
TATTCTCACA ATGATTTAAG TAGACCTCTG GAGGAAGAAT GTCAAATTCT TGAAGAGGAA 
AGGCTGATAT CACTAGTATT TGGACTTCTA AAACAAAGGA AGCTGAATTT TTTTGAAATA 
TATGGAGATG AAATTATTGT CACAGCAAAA AATATAATAA AACAGTGTGT TGTTAACATA 
GTATCACAAA TAGAAGAAAT TGACACAGAT GTTGTTGTAA AACTTGCAGA TCAAATGAGG 
ATGTTGAATT TTCCACAGTG GTTTGAACTT CTGAAAAATA TTTTTTCTAA TTTTATTGTC 
TTTCTCAAGA GAATTAAGGG AACACTAAAT GTCATCAGAA GCGTGGTTCT CTTGGTTCTT 
GACAAGAACC AAAGGAGCAA GGAAGTGGGC GATGTTTCTG TGCAGACGAA TTCTGCAAAG 
GAGAGTACCA TAGACACAGA AGTGGCATAT CTGACTCATG AAGGCATGTT CATAAGTGAT 
GCCTTCAGTG AAGGAGACCT TCCATCCACA GTAGCTGATT CTGCCTCTCA GAGAAACCTT 
TCCCCAGACA GTGAGCCCAG CAGTAGTGAT TCTGTATCTG AAGCTGAATG TACCACTGAT 
TCCTCATCTA GCAAGGAACA GACTTCGTCC AGTGTAACAC TGGGTGGAAT TGAGATTGTG 
ATTGGTGAAG ATATAAAATT GTCAGAACTG GAACTGGGCA GATTAGCAAT TAACATCCAG 
GAGCTACTTT ACAGTGCCTC AGATATTTGC CATGATCGCT CTGTCAAGTT TCTCATGGCT 
AGAGCAAAAG ATGGGTTCCT GGAGAAACTG AATTCTACAG AGTTTGTTGC TCTTTCCCGA 
TTGATGGAAA CTTTCATCTT GGATACAGAA CAGATCTGTG GTAGAAAGAG TATGTCGTTG 
CGTGGTGCAC TTCAGAGCCA AGCTAATAGA TTTGTAAACA GGTTCCATGA GGAAAGAAAG 
ACTAAACTCA GTCTTTTACT AGATAATGAG CGCTGGAAGC AAGCTGACGT TCCTGCAGAA 
TTTCAAGATC TTGTTGATTC CATATCAGAT GGCAAAATTA CTTTACCAGA GAAAAAAGCG 
GGAGCACCTG AAGAAAGAAA GCCCACTGAT TTCCTTATTG TTGATGGACA AAAATATGCT 
GTAGTCGGGA CAGTGTTGCT ATTAATTAGA ATCATCCTTG AATACTGCCA GTGTGTGGAT 
AATATCCCCT CCATAGTGAC TGACATGCTT ACTCGCTTGT CTGATTTACT GAAGTATTTC 
AATTCAAGAA GTTGCCAACT AGTACTTGGA GCAGGTGCTT TACAAGTGGT TGGCTTGAAA 
ACAATAACTA CAAAGAATTT AGCTCTTTCA TCACGCTGCC TACAGCTTAT AGTATACTAC 
ATTCCTGTTA TCAGGGCTCA TTTTGAAGCT CGCCTGCAGC CCAAACAGTT CAGCATGCTT 
AGGCATTTTG ACCACATTAC CAAGGATTAC CATGACCATA TAGCAGAAAT TTCAACTAAA 
CTTGTAGCCA TAATGGATAG CTTGTTTGAC AAATTGCTCT CCAAGTATGA AGTGAAAGCT 
CCCGTACCTT CTGTCTGCTT CAGGAATATT TGTAAGCAGA TGGCAAAGAT GCATGAAGCA 
ATATATGACC TGCTTCCTGA GGAGCAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTTC ACTTAAAGAG ACAGTTGGCC CACTTAAATG TAGTCAATGA TGGAGGACCT 
CAAAATGGAT TGGTCACAGC CGATGTAGCT TTTTACACTG GAAACCTCCA AGCATTGAAA 
GGGCTTCAGA CTCTAGATTT AAACATGGCT GAAATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007435817.1
CDS	44..2977
Translation	MASSHRSSPVPQSKNNDAFFKKEMDSSKCFRPVRTLPDVCPKEPTGDPNSLCDSPSLVVDQHRWTIYHSKVNLPAALNDPRLTKRESDFFTKTWGQDFVDIEIVPSLYLPQINKEHFVTYQQEVAQKEKIHEKCKSICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDPVTFNAVLPWSHFNTASGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIGQIDKVMCEGSLKVLSLSLSRNNCIKAYNKLRLMATVHQTQPTVQLLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSSYSHNDLSRPLEEECQILEEERLISLVFGLLKQRKLNFFEIYGDEIIVTAKNIIKQCVVNIVSQIEEIDTDVVVKLADQMRMLNFPQWFELLKNIFSNFIVFLKRIKGTLNVIRSVVLLVLDKNQRSKEVGDVSVQTNSAKESTIDTEVAYLTHEGMFISDAFSEGDLPSTVADSASQRNLSPDSEPSSSDSVSEAECTTDSSSSKEQTSSSVTLGGIEIVIGEDIKLSELELGRLAINIQELLYSASDICHDRSVKFLMARAKDGFLEKLNSTEFVALSRLMETFILDTEQICGRKSMSLRGALQSQANRFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISDGKITLPEKKAGAPEERKPTDFLIVDGQKYAVVGTVLLLIRIILEYCQCVDNIPSIVTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVYYIPVIRAHFEARLQPKQFSMLRHFDHITKDYHDHIAEISTKLVAIMDSLFDKLLSKYEVKAPVPSVCFRNICKQMAKMHEAIYDLLPEEQTQMLFLRINASYKLHLKRQLAHLNVVNDGGPQNGLVTADVAFYTGNLQALKGLQTLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_007435755.2
Organism	Python bivittatus(Burmese python)
Definition	Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007435755.2

ORF Insert Sequence:

ATGGCCTCAA GCCACCGGTC TTCACCAGTG CCTCAGTCAA AGAACAATGA TGCTTTCTTT 
AAAAAGGAAA TGGATTCTTC CAAATGCTTT CGACCAGTGC GCACACTACC AGATGTATGT 
CCCAAGGAAC CCACAGGTGA TCCTAATAGC TTATGTGACA GCCCATCTTT AGTTGTTGAT 
CAGCATCGAT GGACTATTTA TCATTCCAAA GTAAATCTCC CAGCAGCACT AAATGATCCT 
CGATTAACAA AAAGGGAATC TGATTTCTTT ACAAAAACAT GGGGACAGGA TTTTGTGGAC 
ATTGAAATCG TGCCTTCGCT CTACCTCCCA CAGATCAACA AGGAACATTT TGTAACATAC 
CAACAGGAAG TTGCTCAGAA AGAGAAGATT CATGAGAAAT GTAAGAGTAT TTGTCCTCCT 
AAAGATACTT TTGACAGGAC ATTGTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAA 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATCCCGT AACATTTAAT 
GCAGTATTAC CTTGGTCTCA TTTTAATACT GCTTCTGGGA AAGGAAACCG TGATGCAGCT 
TCCTCCAAGT TACTTCAAGA AAAGCTGAGC CACTACCTGG ATATTGTGGA AGTAAATATT 
GCTCATCAGA TCTCTCTCCG ATCAGAAGCT TTTTTTCATG CAATGACCTC TCAGCATGAA 
CTGCAAGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATAGGC 
CAAATAGATA AAGTCATGTG CGAAGGATCC CTTAAAGTGT TAAGTTTATC ACTGAGTAGA 
AATAACTGTA TAAAAGCATA TAATAAGCTG AGGTTGATGG CAACTGTACA CCAAACACAA 
CCTACAGTAC AGCTATTGTT GTCCACTTCT GAATTTGTTG GAGCACTAGA TTTAATTGCA 
ACAACACAGG AAGTGCTACA GCAGGAACTT CAGGGCATAC ACAGTTTCCG GCATCTTGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA GTTCTCTTCT 
TATTCTCACA ATGATTTAAG TAGACCTCTG GAGGAAGAAT GTCAAATTCT TGAAGAGGAA 
AGGCTGATAT CACTAGTATT TGGACTTCTA AAACAAAGGA AGCTGAATTT TTTTGAAATA 
TATGGAGATG AAATTATTGT CACAGCAAAA AATATAATAA AACAGTGTGT TGTTAACATA 
GTATCACAAA TAGAAGAAAT TGACACAGAT GTTGTTGTAA AACTTGCAGA TCAAATGAGG 
ATGTTGAATT TTCCACAGTG GTTTGAACTT CTGAAAAATA TTTTTTCTAA TTTTATTGTC 
TTTCTCAAGA GAATTAAGGG AACACTAAAT GTCATCAGAA GCGTGGTTCT CTTGGTTCTT 
GACAAGAACC AAAGGAGCAA GGAAGTGGGC GATGTTTCTG TGCAGACGAA TTCTGCAAAG 
GAGAGTACCA TAGACACAGA AGTGGCATAT CTGACTCATG AAGGCATGTT CATAAGTGAT 
GCCTTCAGTG AAGGAGACCT TCCATCCACA GTAGCTGATT CTGCCTCTCA GAGAAACCTT 
TCCCCAGACA GTGAGCCCAG CAGTAGTGAT TCTGTATCTG AAGCTGAATG TACCACTGAT 
TCCTCATCTA GCAAGGAACA GACTTCGTCC AGTGTAACAC TGGGTGGAAT TGAGATTGTG 
ATTGGTGAAG ATATAAAATT GTCAGAACTG GAACTGGGCA GATTAGCAAT TAACATCCAG 
GAGCTACTTT ACAGTGCCTC AGATATTTGC CATGATCGCT CTGTCAAGTT TCTCATGGCT 
AGAGCAAAAG ATGGGTTCCT GGAGAAACTG AATTCTACAG AGTTTGTTGC TCTTTCCCGA 
TTGATGGAAA CTTTCATCTT GGATACAGAA CAGATCTGTG GTAGAAAGAG TATGTCGTTG 
CGTGGTGCAC TTCAGAGCCA AGCTAATAGA TTTGTAAACA GGTTCCATGA GGAAAGAAAG 
ACTAAACTCA GTCTTTTACT AGATAATGAG CGCTGGAAGC AAGCTGACGT TCCTGCAGAA 
TTTCAAGATC TTGTTGATTC CATATCAGAT GGCAAAATTA CTTTACCAGA GAAAAAAGCG 
GGAGCACCTG AAGAAAGAAA GCCCACTGAT TTCCTTATTG TTGATGGACA AAAATATGCT 
GTAGTCGGGA CAGTGTTGCT ATTAATTAGA ATCATCCTTG AATACTGCCA GTGTGTGGAT 
AATATCCCCT CCATAGTGAC TGACATGCTT ACTCGCTTGT CTGATTTACT GAAGTATTTC 
AATTCAAGAA GTTGCCAACT AGTACTTGGA GCAGGTGCTT TACAAGTGGT TGGCTTGAAA 
ACAATAACTA CAAAGAATTT AGCTCTTTCA TCACGCTGCC TACAGCTTAT AGTATACTAC 
ATTCCTGTTA TCAGGGCTCA TTTTGAAGCT CGCCTGCAGC CCAAACAGTT CAGCATGCTT 
AGGCATTTTG ACCACATTAC CAAGGATTAC CATGACCATA TAGCAGAAAT TTCAACTAAA 
CTTGTAGCCA TAATGGATAG CTTGTTTGAC AAATTGCTCT CCAAGTATGA AGTGAAAGCT 
CCCGTACCTT CTGTCTGCTT CAGGAATATT TGTAAGCAGA TGGCAAAGAT GCATGAAGCA 
ATATATGACC TGCTTCCTGA GGAGCAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTTC ACTTAAAGAG ACAGTTGGCC CACTTAAATG TAGTCAATGA TGGAGGACCT 
CAAAATGGAT TGGTCACAGC CGATGTAGCT TTTTACACTG GAAACCTCCA AGCATTGAAA 
GGGCTTCAGA CTCTAGATTT AAACATGGCT GAAATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS54 ( XM_007435755.1 ) cDNA ORF clone, Python bivittatus(Burmese python) -> XP_007435817.1 Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.

Price & Availability↑

CloneID	OPy13424
Clone ID Related Accession (Same CDS sequence)	XM_007435755.2 , XM_007435755.1
Accession Version	XM_007435755.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-10
Organism	Python bivittatus(Burmese python)
Product	vacuolar protein sorting-associated protein 54
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006535008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Python bivittatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA GCCACCGGTC TTCACCAGTG CCTCAGTCAA AGAACAATGA TGCTTTCTTT 
AAAAAGGAAA TGGATTCTTC CAAATGCTTT CGACCAGTGC GCACACTACC AGATGTATGT 
CCCAAGGAAC CCACAGGTGA TCCTAATAGC TTATGTGACA GCCCATCTTT AGTTGTTGAT 
CAGCATCGAT GGACTATTTA TCATTCCAAA GTAAATCTCC CAGCAGCACT AAATGATCCT 
CGATTAACAA AAAGGGAATC TGATTTCTTT ACAAAAACAT GGGGACAGGA TTTTGTGGAC 
ATTGAAATCG TGCCTTCGCT CTACCTCCCA CAGATCAACA AGGAACATTT TGTAACATAC 
CAACAGGAAG TTGCTCAGAA AGAGAAGATT CATGAGAAAT GTAAGAGTAT TTGTCCTCCT 
AAAGATACTT TTGACAGGAC ATTGTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAA 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATCCCGT AACATTTAAT 
GCAGTATTAC CTTGGTCTCA TTTTAATACT GCTTCTGGGA AAGGAAACCG TGATGCAGCT 
TCCTCCAAGT TACTTCAAGA AAAGCTGAGC CACTACCTGG ATATTGTGGA AGTAAATATT 
GCTCATCAGA TCTCTCTCCG ATCAGAAGCT TTTTTTCATG CAATGACCTC TCAGCATGAA 
CTGCAAGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATAGGC 
CAAATAGATA AAGTCATGTG CGAAGGATCC CTTAAAGTGT TAAGTTTATC ACTGAGTAGA 
AATAACTGTA TAAAAGCATA TAATAAGCTG AGGTTGATGG CAACTGTACA CCAAACACAA 
CCTACAGTAC AGCTATTGTT GTCCACTTCT GAATTTGTTG GAGCACTAGA TTTAATTGCA 
ACAACACAGG AAGTGCTACA GCAGGAACTT CAGGGCATAC ACAGTTTCCG GCATCTTGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA GTTCTCTTCT 
TATTCTCACA ATGATTTAAG TAGACCTCTG GAGGAAGAAT GTCAAATTCT TGAAGAGGAA 
AGGCTGATAT CACTAGTATT TGGACTTCTA AAACAAAGGA AGCTGAATTT TTTTGAAATA 
TATGGAGATG AAATTATTGT CACAGCAAAA AATATAATAA AACAGTGTGT TGTTAACATA 
GTATCACAAA TAGAAGAAAT TGACACAGAT GTTGTTGTAA AACTTGCAGA TCAAATGAGG 
ATGTTGAATT TTCCACAGTG GTTTGAACTT CTGAAAAATA TTTTTTCTAA TTTTATTGTC 
TTTCTCAAGA GAATTAAGGG AACACTAAAT GTCATCAGAA GCGTGGTTCT CTTGGTTCTT 
GACAAGAACC AAAGGAGCAA GGAAGTGGGC GATGTTTCTG TGCAGACGAA TTCTGCAAAG 
GAGAGTACCA TAGACACAGA AGTGGCATAT CTGACTCATG AAGGCATGTT CATAAGTGAT 
GCCTTCAGTG AAGGAGACCT TCCATCCACA GTAGCTGATT CTGCCTCTCA GAGAAACCTT 
TCCCCAGACA GTGAGCCCAG CAGTAGTGAT TCTGTATCTG AAGCTGAATG TACCACTGAT 
TCCTCATCTA GCAAGGAACA GACTTCGTCC AGTGTAACAC TGGGTGGAAT TGAGATTGTG 
ATTGGTGAAG ATATAAAATT GTCAGAACTG GAACTGGGCA GATTAGCAAT TAACATCCAG 
GAGCTACTTT ACAGTGCCTC AGATATTTGC CATGATCGCT CTGTCAAGTT TCTCATGGCT 
AGAGCAAAAG ATGGGTTCCT GGAGAAACTG AATTCTACAG AGTTTGTTGC TCTTTCCCGA 
TTGATGGAAA CTTTCATCTT GGATACAGAA CAGATCTGTG GTAGAAAGAG TATGTCGTTG 
CGTGGTGCAC TTCAGAGCCA AGCTAATAGA TTTGTAAACA GGTTCCATGA GGAAAGAAAG 
ACTAAACTCA GTCTTTTACT AGATAATGAG CGCTGGAAGC AAGCTGACGT TCCTGCAGAA 
TTTCAAGATC TTGTTGATTC CATATCAGAT GGCAAAATTA CTTTACCAGA GAAAAAAGCG 
GGAGCACCTG AAGAAAGAAA GCCCACTGAT TTCCTTATTG TTGATGGACA AAAATATGCT 
GTAGTCGGGA CAGTGTTGCT ATTAATTAGA ATCATCCTTG AATACTGCCA GTGTGTGGAT 
AATATCCCCT CCATAGTGAC TGACATGCTT ACTCGCTTGT CTGATTTACT GAAGTATTTC 
AATTCAAGAA GTTGCCAACT AGTACTTGGA GCAGGTGCTT TACAAGTGGT TGGCTTGAAA 
ACAATAACTA CAAAGAATTT AGCTCTTTCA TCACGCTGCC TACAGCTTAT AGTATACTAC 
ATTCCTGTTA TCAGGGCTCA TTTTGAAGCT CGCCTGCAGC CCAAACAGTT CAGCATGCTT 
AGGCATTTTG ACCACATTAC CAAGGATTAC CATGACCATA TAGCAGAAAT TTCAACTAAA 
CTTGTAGCCA TAATGGATAG CTTGTTTGAC AAATTGCTCT CCAAGTATGA AGTGAAAGCT 
CCCGTACCTT CTGTCTGCTT CAGGAATATT TGTAAGCAGA TGGCAAAGAT GCATGAAGCA 
ATATATGACC TGCTTCCTGA GGAGCAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTTC ACTTAAAGAG ACAGTTGGCC CACTTAAATG TAGTCAATGA TGGAGGACCT 
CAAAATGGAT TGGTCACAGC CGATGTAGCT TTTTACACTG GAAACCTCCA AGCATTGAAA 
GGGCTTCAGA CTCTAGATTT AAACATGGCT GAAATCTGGG AACAGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007435817.1
CDS	17..2950
Translation	MASSHRSSPVPQSKNNDAFFKKEMDSSKCFRPVRTLPDVCPKEPTGDPNSLCDSPSLVVDQHRWTIYHSKVNLPAALNDPRLTKRESDFFTKTWGQDFVDIEIVPSLYLPQINKEHFVTYQQEVAQKEKIHEKCKSICPPKDTFDRTLLHTHDKSRTDLEQVPKIFMKPDFALDDPVTFNAVLPWSHFNTASGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIGQIDKVMCEGSLKVLSLSLSRNNCIKAYNKLRLMATVHQTQPTVQLLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSSYSHNDLSRPLEEECQILEEERLISLVFGLLKQRKLNFFEIYGDEIIVTAKNIIKQCVVNIVSQIEEIDTDVVVKLADQMRMLNFPQWFELLKNIFSNFIVFLKRIKGTLNVIRSVVLLVLDKNQRSKEVGDVSVQTNSAKESTIDTEVAYLTHEGMFISDAFSEGDLPSTVADSASQRNLSPDSEPSSSDSVSEAECTTDSSSSKEQTSSSVTLGGIEIVIGEDIKLSELELGRLAINIQELLYSASDICHDRSVKFLMARAKDGFLEKLNSTEFVALSRLMETFILDTEQICGRKSMSLRGALQSQANRFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSISDGKITLPEKKAGAPEERKPTDFLIVDGQKYAVVGTVLLLIRIILEYCQCVDNIPSIVTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVYYIPVIRAHFEARLQPKQFSMLRHFDHITKDYHDHIAEISTKLVAIMDSLFDKLLSKYEVKAPVPSVCFRNICKQMAKMHEAIYDLLPEEQTQMLFLRINASYKLHLKRQLAHLNVVNDGGPQNGLVTADVAFYTGNLQALKGLQTLDLNMAEIWEQKR

Target ORF information:

RefSeq Version	XM_007435755.1
Organism	Python bivittatus(Burmese python)
Definition	Python bivittatus VPS54, GARP complex subunit (VPS54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007435755.1

ORF Insert Sequence:

ATGGCCTCAA GCCACCGGTC TTCACCAGTG CCTCAGTCAA AGAACAATGA TGCTTTCTTT 
AAAAAGGAAA TGGATTCTTC CAAATGCTTT CGACCAGTGC GCACACTACC AGATGTATGT 
CCCAAGGAAC CCACAGGTGA TCCTAATAGC TTATGTGACA GCCCATCTTT AGTTGTTGAT 
CAGCATCGAT GGACTATTTA TCATTCCAAA GTAAATCTCC CAGCAGCACT AAATGATCCT 
CGATTAACAA AAAGGGAATC TGATTTCTTT ACAAAAACAT GGGGACAGGA TTTTGTGGAC 
ATTGAAATCG TGCCTTCGCT CTACCTCCCA CAGATCAACA AGGAACATTT TGTAACATAC 
CAACAGGAAG TTGCTCAGAA AGAGAAGATT CATGAGAAAT GTAAGAGTAT TTGTCCTCCT 
AAAGATACTT TTGACAGGAC ATTGTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAA 
CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATCCCGT AACATTTAAT 
GCAGTATTAC CTTGGTCTCA TTTTAATACT GCTTCTGGGA AAGGAAACCG TGATGCAGCT 
TCCTCCAAGT TACTTCAAGA AAAGCTGAGC CACTACCTGG ATATTGTGGA AGTAAATATT 
GCTCATCAGA TCTCTCTCCG ATCAGAAGCT TTTTTTCATG CAATGACCTC TCAGCATGAA 
CTGCAAGACT ACCTCAAGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATAGGC 
CAAATAGATA AAGTCATGTG CGAAGGATCC CTTAAAGTGT TAAGTTTATC ACTGAGTAGA 
AATAACTGTA TAAAAGCATA TAATAAGCTG AGGTTGATGG CAACTGTACA CCAAACACAA 
CCTACAGTAC AGCTATTGTT GTCCACTTCT GAATTTGTTG GAGCACTAGA TTTAATTGCA 
ACAACACAGG AAGTGCTACA GCAGGAACTT CAGGGCATAC ACAGTTTCCG GCATCTTGGA 
TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA GTTCTCTTCT 
TATTCTCACA ATGATTTAAG TAGACCTCTG GAGGAAGAAT GTCAAATTCT TGAAGAGGAA 
AGGCTGATAT CACTAGTATT TGGACTTCTA AAACAAAGGA AGCTGAATTT TTTTGAAATA 
TATGGAGATG AAATTATTGT CACAGCAAAA AATATAATAA AACAGTGTGT TGTTAACATA 
GTATCACAAA TAGAAGAAAT TGACACAGAT GTTGTTGTAA AACTTGCAGA TCAAATGAGG 
ATGTTGAATT TTCCACAGTG GTTTGAACTT CTGAAAAATA TTTTTTCTAA TTTTATTGTC 
TTTCTCAAGA GAATTAAGGG AACACTAAAT GTCATCAGAA GCGTGGTTCT CTTGGTTCTT 
GACAAGAACC AAAGGAGCAA GGAAGTGGGC GATGTTTCTG TGCAGACGAA TTCTGCAAAG 
GAGAGTACCA TAGACACAGA AGTGGCATAT CTGACTCATG AAGGCATGTT CATAAGTGAT 
GCCTTCAGTG AAGGAGACCT TCCATCCACA GTAGCTGATT CTGCCTCTCA GAGAAACCTT 
TCCCCAGACA GTGAGCCCAG CAGTAGTGAT TCTGTATCTG AAGCTGAATG TACCACTGAT 
TCCTCATCTA GCAAGGAACA GACTTCGTCC AGTGTAACAC TGGGTGGAAT TGAGATTGTG 
ATTGGTGAAG ATATAAAATT GTCAGAACTG GAACTGGGCA GATTAGCAAT TAACATCCAG 
GAGCTACTTT ACAGTGCCTC AGATATTTGC CATGATCGCT CTGTCAAGTT TCTCATGGCT 
AGAGCAAAAG ATGGGTTCCT GGAGAAACTG AATTCTACAG AGTTTGTTGC TCTTTCCCGA 
TTGATGGAAA CTTTCATCTT GGATACAGAA CAGATCTGTG GTAGAAAGAG TATGTCGTTG 
CGTGGTGCAC TTCAGAGCCA AGCTAATAGA TTTGTAAACA GGTTCCATGA GGAAAGAAAG 
ACTAAACTCA GTCTTTTACT AGATAATGAG CGCTGGAAGC AAGCTGACGT TCCTGCAGAA 
TTTCAAGATC TTGTTGATTC CATATCAGAT GGCAAAATTA CTTTACCAGA GAAAAAAGCG 
GGAGCACCTG AAGAAAGAAA GCCCACTGAT TTCCTTATTG TTGATGGACA AAAATATGCT 
GTAGTCGGGA CAGTGTTGCT ATTAATTAGA ATCATCCTTG AATACTGCCA GTGTGTGGAT 
AATATCCCCT CCATAGTGAC TGACATGCTT ACTCGCTTGT CTGATTTACT GAAGTATTTC 
AATTCAAGAA GTTGCCAACT AGTACTTGGA GCAGGTGCTT TACAAGTGGT TGGCTTGAAA 
ACAATAACTA CAAAGAATTT AGCTCTTTCA TCACGCTGCC TACAGCTTAT AGTATACTAC 
ATTCCTGTTA TCAGGGCTCA TTTTGAAGCT CGCCTGCAGC CCAAACAGTT CAGCATGCTT 
AGGCATTTTG ACCACATTAC CAAGGATTAC CATGACCATA TAGCAGAAAT TTCAACTAAA 
CTTGTAGCCA TAATGGATAG CTTGTTTGAC AAATTGCTCT CCAAGTATGA AGTGAAAGCT 
CCCGTACCTT CTGTCTGCTT CAGGAATATT TGTAAGCAGA TGGCAAAGAT GCATGAAGCA 
ATATATGACC TGCTTCCTGA GGAGCAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT 
TACAAACTTC ACTTAAAGAG ACAGTTGGCC CACTTAAATG TAGTCAATGA TGGAGGACCT 
CAAAATGGAT TGGTCACAGC CGATGTAGCT TTTTACACTG GAAACCTCCA AGCATTGAAA 
GGGCTTCAGA CTCTAGATTT AAACATGGCT GAAATCTGGG AACAGAAGAG GTGA